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Articles 811 - 840 of 1681
Full-Text Articles in Biology
Identification Of Hla-A*0201-Restricted Ctl Epitopes From The Receptor-Binding Domain Of Mers-Cov Spike Protein Using A Combinatorial In Silico Approach, Naghmeh Poorinmohammad, Hassan Mohabatkar
Identification Of Hla-A*0201-Restricted Ctl Epitopes From The Receptor-Binding Domain Of Mers-Cov Spike Protein Using A Combinatorial In Silico Approach, Naghmeh Poorinmohammad, Hassan Mohabatkar
Turkish Journal of Biology
A novel SARS-like illness called Middle East respiratory syndrome coronavirus (MERS-CoV), caused by an emerging coronavirus, has been a recent cause for concern due to its fatality and pandemic potential. Developing a peptide-based vaccine could be helpful in fighting against the virus. Since the experimental procedure is time-consuming and expensive, computational analysis can play an important role in accelerating the process. Therefore, the aim of this study was to computationally identify cytotoxic T-lymphocyte epitopes presented by the human leukocyte antigen (HLA)-A*0201, as this is the most frequent HLA class I allele among Middle Eastern populations. The receptor-binding domain of the …
Bacterial Toxin Colicin N-T Domain Structure Changes To Ordered State Upon Binding C-Terminal Domain Of Tola, Yakup Ulusu, Sema Bi̇lgi̇n Şentürk, Fatma Gedi̇kli̇, Jeremy H. Lakey, İsa Gökçe
Bacterial Toxin Colicin N-T Domain Structure Changes To Ordered State Upon Binding C-Terminal Domain Of Tola, Yakup Ulusu, Sema Bi̇lgi̇n Şentürk, Fatma Gedi̇kli̇, Jeremy H. Lakey, İsa Gökçe
Turkish Journal of Biology
Colicin N is a bacterial toxin that kills Escherichia coli and related cells. Its mode of action is of interest in protein import and toxicology. Colicin N translocates across the E. coli outer membrane and periplasm by interacting with several receptors. The translocation process involves the interaction of the colicin N with the outer membrane porin OmpF and subsequently with the integral membrane protein TolA. The N-terminal domain of colicin N is involved in the import process. TolA consists of 3 domains. The N-terminal domain of colicin N interacts with the C-terminal domain of TolA at later stages of the …
In Vitro Regeneration And Conservation Of The Lentisk (Pistacia Lentiscus L.), İbrahi̇m Koç, Ahmet Onay, Yelda Özden Çi̇ftçi̇
In Vitro Regeneration And Conservation Of The Lentisk (Pistacia Lentiscus L.), İbrahi̇m Koç, Ahmet Onay, Yelda Özden Çi̇ftçi̇
Turkish Journal of Biology
Pistacia lentiscus L. (lentisk or mastic tree) is an economically important member of the genus Pistacia due to its valuable mastic resin. Shoot tips and nodal segments were used as explant sources from in vitro-germinated seeds of lentisk. Shoot tips were found to be suitable for multiple shoot formation. Thereafter, the influences of different growth regulators [N6-benzyladenine (BA), gibberellic acid (GA_3), naphthalene acetic acid (NAA), or jasmonic acid (JA)] together with various elicitors [silver nitrate (AgNO_3) or phloroglucinol (PG)] were assessed to develop efficient micropropagation protocol. Our results showed that the combination of all tested concentrations of GA_3 with 1.0 …
Cloning And Expression Analysis Of 1-Deoxy-D-Xylulose-5-Phosphate Synthase Gene From The Medicinal Plant Conyza Blinii H.Lév., Rong Sun, Shan Liu, Jing-Lei Gao, Zi-Zhong Tang, Hui Chen, Cheng-Lei Li, Qi Wu
Cloning And Expression Analysis Of 1-Deoxy-D-Xylulose-5-Phosphate Synthase Gene From The Medicinal Plant Conyza Blinii H.Lév., Rong Sun, Shan Liu, Jing-Lei Gao, Zi-Zhong Tang, Hui Chen, Cheng-Lei Li, Qi Wu
Turkish Journal of Biology
Conyza blinii H.Lév. is a traditional Chinese medicinal plant that is distributed mainly in southwestern Sichuan and northern Yunnan. Its characteristic product is blinin, which has, among other properties, antigastric ulcer activity, and can serve as a quality-control standard for such medicine. The problem is that C. blinii only produces low yields of blinin. As a diterpene, blinin is likely formed by the methylerythritol phosphate pathway. While 1-deoxy-D-xylulose-5-phosphate synthase (DXS) is the first rate-limiting enzyme in diterpenoid biosynthesis, it is a switch in the pathway. The DXS gene was successfully cloned and characterized from C. blinii by homologous cloning and …
Phylogenetic Analysis Of Peste Des Petits Ruminants Virus From Outbreaks In Turkey During 2008-2012, Leyla Güler, Murat Şevi̇k, Mustafa Hasöksüz
Phylogenetic Analysis Of Peste Des Petits Ruminants Virus From Outbreaks In Turkey During 2008-2012, Leyla Güler, Murat Şevi̇k, Mustafa Hasöksüz
Turkish Journal of Biology
Peste des petits ruminants (PPR) is an important viral disease of sheep and goats and is endemic in all regions of Turkey. In this study, PPR virus infection was investigated by RT-PCR assay based on the fusion (F) gene in PPR-suspected sheep and goat samples. PPR virus RNA was detected in 65 small ruminants (51 sheep, 14 goats) from independent outbreaks during 2008-2012 in provinces in the central and Mediterranean regions and the central-west part of the Aegean region in Turkey. The virus was detected in an aborted sheep fetus sample by RT-PCR, and diagnosis was also confirmed by virus …
Computational Regulatory Model For Detoxification Of Ammonia From Urea Cycle In Liver, Rashith Muhammad Mubarak Ali, Poornima Devi Gurusamy, Selvakumar Ramachandran
Computational Regulatory Model For Detoxification Of Ammonia From Urea Cycle In Liver, Rashith Muhammad Mubarak Ali, Poornima Devi Gurusamy, Selvakumar Ramachandran
Turkish Journal of Biology
A nondeterministic finite automaton was designed to monitor enzymatic regulation and detoxification of excess ammonia in the urea cycle and its disorders. The designed machine is used for the diagnosis of deficiency and for regulating the expression of any of the enzymes involved with acceptance and rejection states in the urea cycle. The urea cycle is the metabolism of excess nitrogen produced by the breakdown of protein and other nitrogen-containing molecules in liver. Disorder in the urea cycle may lead to the accumulation of toxic ammonia in the blood, which leads to hyperammonemia. The elevation of plasma ammonia concentration may …
Biodegradation Of The Synthetic Pyrethroid Insecticide Α-Cypermethrin By Stenotrophomonas Maltophilia Og2, Özlem Gür, Murat Özdal, Ömer Faruk Algur
Biodegradation Of The Synthetic Pyrethroid Insecticide Α-Cypermethrin By Stenotrophomonas Maltophilia Og2, Özlem Gür, Murat Özdal, Ömer Faruk Algur
Turkish Journal of Biology
A novel gram-negative bacterium, OG2, was isolated from the body microflora of cockroaches (Blatta orientalis). Based on morphological, biochemical, and 16S ribosomal DNA sequence analysis, the isolated strain OG2 was identified as Stenotrophomonas maltophilia. This bacterial strain was screened for its alpha-cypermethrin-degrading potential with minimal salt medium (MSM). The alpha-cypermethrin degradation and utilization ability of the isolated organism was verified. Bacterial growth was measured by optical density in the presence of the pesticide at different concentrations (50-200 mg/L). S. maltophilia OG2 utilized alpha-cypermethrin as the sole carbon source for growth, and alpha-cypermethrin degradation increased when MSM was supplemented with glucose. …
Nitrate And Iron Nutrition Effects On Some Nitrate Assimilation Enzymes And Metabolites In Spirulina Platensis, Merve Esen, Razi̇ye Öztürk Ürek
Nitrate And Iron Nutrition Effects On Some Nitrate Assimilation Enzymes And Metabolites In Spirulina Platensis, Merve Esen, Razi̇ye Öztürk Ürek
Turkish Journal of Biology
Effects of different sodium nitrate and iron concentrations on the production of some metabolites and pigments and the activities of some nitrate assimilation enzymes [nitrate reductase (NR), nitrite reductase (NiR), glutamine synthetase (GS), and glutamate synthase (GOGAT)] were investigated in the growth medium of Spirulina platensis (Gamont) Geitler. For this purpose, S. platensis was cultivated on Zarrouk's medium containing different sodium nitrate concentrations (10-180 mM). A 100 mM sodium nitrate concentration stimulated growth of this organism as well as the production of pigments and other metabolites and enzyme activities. In the presence of 100 mM sodium nitrate, different concentrations of …
Triumph Or Tragedy: Progress In Cancer, Ayşe Şebnem Erenler, Hi̇kmet Geçki̇l
Triumph Or Tragedy: Progress In Cancer, Ayşe Şebnem Erenler, Hi̇kmet Geçki̇l
Turkish Journal of Biology
Cancer is probably the number one research area among all human endeavors, receiving the largest portion of science funding in most countries. This is because cancer remains one of the oldest conundrums among all human maladies. Although we now have a greater understanding of the biological and molecular basis of cancer, its diagnosis and therapy still pose great challenges. In this review, our aim is not to establish a comprehensive understanding of cancer, which is essentially impossible, but to outline, in a more provocative way, why cancer research in the pursuit of a cure did not live up to its …
Aging And Cancer: Molecular Facts And Awareness For Turkey, Gülgün Gündüz, Kayahan Fişkin
Aging And Cancer: Molecular Facts And Awareness For Turkey, Gülgün Gündüz, Kayahan Fişkin
Turkish Journal of Biology
Two important subjects concerning extension of the human life-span are biological aging processes and their relationship to diseases, and especially to the formation of cancer. It is clear that an aging population poses some challenges for developing countries. We set out to show the demographic structure of Turkey and to discuss the feasibility of decreasing the prevalence of cancer. We overviewed the literature on the relationship between biological aging and cancer formation, the environmental causes of cancer, and age-related characteristics of cancer in the elderly. In addition, some statistical data on the changing demography of Turkey were reviewed. It is …
Autophagy And Cancer, Hacer Ezgi̇ Karakaş, Devri̇m Gözüaçik
Autophagy And Cancer, Hacer Ezgi̇ Karakaş, Devri̇m Gözüaçik
Turkish Journal of Biology
Autophagy is an evolutionary conserved intracellular degradation and stress response mechanism that is mainly responsible for the breakdown and recycling of cytoplasmic materials, including long-lived proteins, protein aggregates, and damaged organelles. In this way, autophagy provides the cell with building blocks and allows the maintenance of homeostasis under stress conditions such as growth factor deficiency, nutrient deprivation, hypoxia, and toxins. Consequently, abnormalities of autophagy contribute to a number of pathologies ranging from neurodegenerative diseases to cancer. Autophagy was reported to have a dual role in cancer. Depending on cancer stage, autophagy seems to act as tumor suppressor or as a …
Invadopodia: Proteolytic Feet Of Cancer Cells, Gi̇zem Bati, Devri̇m Pesen Okvur
Invadopodia: Proteolytic Feet Of Cancer Cells, Gi̇zem Bati, Devri̇m Pesen Okvur
Turkish Journal of Biology
The leading cause of death in cancer patients is metastasis. Invasion is an integral part of metastasis and is carried out by proteolytic structures called invadopodia at the cellular level. In this introductory review, we start by evaluating the definition of invadopodia. While presenting the upstream signaling events involved, we integrate current models on invadopodia. In addition, we discuss the significance of invadopodia in 2D and 3D and in vivo. We finally point out technical challenges and conclude with open questions in the field.
It Takes 2 Antioxidants To Tango: The Interaction Between Manganese Superoxide Dismutase And Glutathione Peroxidase-1, Dede N. Ekoue, Alan M. Diamond
It Takes 2 Antioxidants To Tango: The Interaction Between Manganese Superoxide Dismutase And Glutathione Peroxidase-1, Dede N. Ekoue, Alan M. Diamond
Turkish Journal of Biology
Reactive oxygen species (ROS) are generated during mitochondrial oxidative metabolism. Accumulation of ROS without an effective antioxidant response can lead to oxidative stress, resulting in macromolecular damage that is implicated in the etiology of various diseases including cancer. ROS detoxification is regulated by various antioxidant proteins, specifically manganese superoxide dismutase (MnSOD), which catalyzes the conversion of superoxide into H_{2}O_{2}, and the subsequent conversion of H_{2}O_{2} into water is catalyzed by glutathione peroxidase 1 (GPx-1). In vitro and in vivo evidence supports a conflicting role of MnSOD in tumor biology and indicates that an interaction between MnSOD and GPx-1 can modulate …
Glycosylation Changes Leading To The Increase In Size On The Common Core Of N-Glycans, Required Enzymes, And Related Cancer-Associated Proteins, Sabi̇re Karaçali, Savaş İzzetoğlu, Remzi̇ye Deveci̇
Glycosylation Changes Leading To The Increase In Size On The Common Core Of N-Glycans, Required Enzymes, And Related Cancer-Associated Proteins, Sabi̇re Karaçali, Savaş İzzetoğlu, Remzi̇ye Deveci̇
Turkish Journal of Biology
Glycan parts of glycoconjugates on the surfaces of cells regulate many kinds of interactions between the cells and their immediate environments. Alterations in glycosylation on the cancer-associated glycoproteins are responsible for changes in their molecular interactions and biological functions. Glycosylation changes occur in the core and/or at the nonreducing end of the oligosaccharide chains of N-glycans. In this review, we focus on the branching of the common core structure of N-glycans, the responsible enzyme, and the extensions of some of the branches causing size increases on the surface of tumor cells. Abnormal branching, elongation of the branches, and increasing size …
Failure Of Immunological Cells To Eradicate Tumor And Cancer Cells: An Overview, Rohit Sharma, Daizee Talukdar, Parth Malik, Tapan K. Mukherjee
Failure Of Immunological Cells To Eradicate Tumor And Cancer Cells: An Overview, Rohit Sharma, Daizee Talukdar, Parth Malik, Tapan K. Mukherjee
Turkish Journal of Biology
Inflammation can be broadly understood as a successive immune response of an organism's immune system towards nonnative or foreign antigens. This is a protective mechanism of the immune system, mediated by diverse immunological cells, to ensure homeostasis of an individual. Once activated, these immunological cells release a number of cytokines, chemokines, reactive oxygen species, reactive nitrogen species, histamines, prostaglandins, and other materials leading to inflammation. Tumor cells express altered proteins due to mutations of their genes, DNA modifications such as histone modification, DNA methylation, or other mechanisms of altered protein expression. The body's immunological cells actively recognize these altered proteins, …
The Role Of Abc Transporters In Anticancer Drug Transport, Serhan Karvar
The Role Of Abc Transporters In Anticancer Drug Transport, Serhan Karvar
Turkish Journal of Biology
The main clinical problem in cancer treatment is the development of multidrug resistance. Tumors can be intrinsically drug-resistant or develop resistance to chemotherapy. Multidrug resistance can develop by diverse mechanisms including decreased rate of drug uptake, increased drug efflux, alterations in drug metabolism, mutation of drug targets, activation of DNA repair mechanisms, and evasion of apoptosis. The use of modern genomic, proteomic, bioinformatic, and systems biology approaches has resulted in a substantial increase in our ability to identify molecular mechanisms that are involved in multidrug resistance in cancer and to find drugs that may block or reverse the development of …
Multidrug Resistance In Chronic Myeloid Leukemia, Mi̇ray Ünlü, Yağmur Ki̇raz, Fatma Necmi̇ye Kaci, Mehmet Ali̇ Özcan, Yusuf Baran
Multidrug Resistance In Chronic Myeloid Leukemia, Mi̇ray Ünlü, Yağmur Ki̇raz, Fatma Necmi̇ye Kaci, Mehmet Ali̇ Özcan, Yusuf Baran
Turkish Journal of Biology
Chronic myeloid leukemia (CML) is characterized by the accumulation of Philadelphia chromosome-positive (Ph+) myeloid cells. Ph+ cells occur via a reciprocal translocation between the long arms of chromosomes 9 and 22 resulting in constitutively active Bcr-abl fusion protein. Tyrosine kinase inhibitors (TKIs) are used against the kinase activity of Bcr-abl fusion protein for the effective treatment of CML. However, the development of drug resistance, directed by different genetic mechanisms, is the major problem of clinical applications of TKIs. These mechanisms include mutations in the TKI binding site of Bcr-abl, overexpression of Bcr-abl, overexpression of ATP binding cassette transporters, aberrant ceramide …
Noncoding Rnas And Cancer, Hesna Begüm Akman Tuncer, Ayşe Eli̇f Erson Bensan
Noncoding Rnas And Cancer, Hesna Begüm Akman Tuncer, Ayşe Eli̇f Erson Bensan
Turkish Journal of Biology
New and diverse groups of noncoding RNAs are beginning to be discovered. These noncoding RNAs are grouped based on their sizes, genomic positions, or distinctive functions ranging from the regulation of chromatin structure and gene expression to genome stability maintenance. Among noncoding RNAs, microRNAs are the best studied and understood small RNAs with direct and indirect roles in normal and in cancer cells. Given the high rate of transcription from noncoding parts of the genome, other noncoding elements are also of great interest. It is also of interest to understand that there may be interesting cases of crosstalk and/or coregulation …
Cancer Stem Cells: Emerging Actors In Both Basic And Clinical Cancer Research, Di̇dem Karakaş, Buse Cevatemre, Engi̇n Ulukaya
Cancer Stem Cells: Emerging Actors In Both Basic And Clinical Cancer Research, Di̇dem Karakaş, Buse Cevatemre, Engi̇n Ulukaya
Turkish Journal of Biology
Cancer stem cells (CSCs) are a small subset of cancer cells within a tumor that are responsible for tumorigenesis and contribute to drug resistance. The CSC displays an anchorage-independent survival, active DNA-repair capacity, and relative quiescence and is capable of self-renewing and maintaining tumor growth and heterogeneity. At the molecular level, there are several signaling pathways (e.g., Wnt/Beta-catenin, Notch, and Hedgehog) to control CSC properties and alteration of these pathways has been recognized as an essential step for CSC transformation. Emerging evidence suggests that CSCs are clinically relevant. These cells are resistant to conventional chemotherapy and radiation treatment. Therefore, CSCs …
Evaluation Of Muc1, Ck20, And Htert Expression In Peripheral Blood Of Gastrointestinal Cancer Patients In Search Of Diagnostic Criteria, Si̇bel Küçükyildirim Çeli̇k, Beri̇l Erdem, Emel Sağlar, Zülfi̇kar Polat, Hati̇ce Mergen
Evaluation Of Muc1, Ck20, And Htert Expression In Peripheral Blood Of Gastrointestinal Cancer Patients In Search Of Diagnostic Criteria, Si̇bel Küçükyildirim Çeli̇k, Beri̇l Erdem, Emel Sağlar, Zülfi̇kar Polat, Hati̇ce Mergen
Turkish Journal of Biology
The goal of this study was to identify the optimal marker or marker combinations for detection of gastrointestinal malignancies using reverse-transcriptase polymerase chain (RT-PCR) reaction. To detect the presence of tumors, we analyzed mucin 1 (MUC1), cytokeratin 19 (CK19), cytokeratin 20 (CK20), and human telomerase reverse transcriptase (hTERT) mRNA in the peripheral blood of 31 patients with gastrointestinal (esophagus, stomach, and colorectal) cancer and 30 healthy individuals. In RT-PCR analysis of the peripheral blood, 77.4% (24/31), 61.29% (19/31), and 45.16% (14/31) of cancer patients were positive for MUC1, CK20, and hTERT mRNA, respectively. According to our results, any one of …
The Effect Of Survivin Gene Promoter Polymorphism On Breast Cancer, Mehmet Deni̇z Altiparmak, Celal İsmai̇l Bi̇lgi̇ç, Nüzhet Cenap Dener, Esra Gündüz, Si̇bel Yeni̇dünya, Muradi̇ye Acar, Meral Şen, Mehmet Gündüz
The Effect Of Survivin Gene Promoter Polymorphism On Breast Cancer, Mehmet Deni̇z Altiparmak, Celal İsmai̇l Bi̇lgi̇ç, Nüzhet Cenap Dener, Esra Gündüz, Si̇bel Yeni̇dünya, Muradi̇ye Acar, Meral Şen, Mehmet Gündüz
Turkish Journal of Biology
Breast cancer is the most common malignant tumor in women and accounts for about 25% of all cancer diagnoses. Survivin is a member of the apoptosis inhibitor protein family of antiapoptotic proteins. In our study, we investigated one of those, the survivin gene promoter 31G/C polymorphism. Included in this study were 111 breast cancer patients who were operated on in our hospital and 101 healthy female subjects. Blood samples from the healthy subjects and paraffin-embedded tissue samples from the patients were used for DNA extraction and subsequent genetic analysis. PCR-RFLP was used for genotype analysis. We established the clinicopathologic characteristics …
Downregulation Of C-Myc Mediated Odc Expression After Purvalanol Treatment Is Under Control Of Upstream Mapk Signaling Axis In Mcf-7 Breast Cancer Cells, Pinar Obakan, Gi̇zem Alkurt, Betsi̇ Köse, Ajda Çoker Gürkan, Eli̇f Damla Arisan, Deni̇z Coşkun, Zeynep Narçi̇n Ünsal
Downregulation Of C-Myc Mediated Odc Expression After Purvalanol Treatment Is Under Control Of Upstream Mapk Signaling Axis In Mcf-7 Breast Cancer Cells, Pinar Obakan, Gi̇zem Alkurt, Betsi̇ Köse, Ajda Çoker Gürkan, Eli̇f Damla Arisan, Deni̇z Coşkun, Zeynep Narçi̇n Ünsal
Turkish Journal of Biology
Roscovitine and purvalanol are specific cyclin-dependent kinase (CDK) inhibitors, which induce apoptosis by triggering cell cycle arrest in various cancer cells such as colon, prostate, and breast cancer cells. Although the apoptotic action of roscovitine was clarified at the molecular level, the exact mechanism of purvalanol-induced apoptosis is still under investigation. The mitogen-activated protein kinase (MAPK) signaling cascade is activated by different inducers related to growth, proliferation, differentiation processes, or environmental stress factors. Recent reports showed that modulation of MAPKs might lead to regulation of c-Myc, which is a transcription factor for the polyamine (PA) biosynthesis enzyme, ornithine decarboxylase (ODC). …
Identification Of New Genes Required For The Maintenance Of Chromosome Integrity In Drosophila Melanogaster, Francesca Ceprani, Franco Spirito, Roberto Piergentili
Identification Of New Genes Required For The Maintenance Of Chromosome Integrity In Drosophila Melanogaster, Francesca Ceprani, Franco Spirito, Roberto Piergentili
Turkish Journal of Biology
Although genome-wide RNA interference (RNAi) screens for mitotic genes are not new in the literature, most of them lack the cytological characterization of the cell karyotype as for chromosome integrity. Here, the effects of RNAi on chromosome structure in S2 cultured cells of Drosophila melanogaster were analyzed. The cytological phenotype of 1132 genes selected by coexpression with known mitotic genes was scored. Cytological and statistical analysis of the treated cells allowed the identifying of 81 loci whose inactivation brings a level of chromosome breakage significantly higher than in the control. Many of the genes characterized in the present work had …
Analysis Of “Bimbam”, A Novel Glucocorticoid-Induced Bh3-Only Transcript In Cell Lines And Children With Acute Lymphoblastic Leukemia, Muhammad Mansha, Muhammad Wasim, Ali Raza Awan, Asma Abdul Latif
Analysis Of “Bimbam”, A Novel Glucocorticoid-Induced Bh3-Only Transcript In Cell Lines And Children With Acute Lymphoblastic Leukemia, Muhammad Mansha, Muhammad Wasim, Ali Raza Awan, Asma Abdul Latif
Turkish Journal of Biology
Glucocorticoids (GCs) induce apoptosis and cell cycle arrest in lymphoid cells and are fundamental components of therapeutic regimens of acute lymphoblastic leukemia (ALL). We previously identified a panel of GC-induced candidate genes in children with ALL and in other biological systems using whole genome expression profiling. Of those, a novel transcript from the BCL2L11/Bim locus, named \"Bimbam\", was functionally analyzed in the current study. The postulated Bimbam mRNA consists of the 5' portion of Bim including the BH3-containing exon 8 and the 3' portion of Bam, a small 2-exon gene embedded in the BCL2L11/Bim locus. The expression and GC induction …
Cytotoxic And Apoptotic Effects Of Ceranib-2 Offering Potential For A New Antineoplastic Agent In The Treatment Of Cancer Cells, Djanan Vejselova, Hati̇ce Mehtap Kutlu, Gökhan Kuş, Selda Kabadere, Ruhi̇ Uyar
Cytotoxic And Apoptotic Effects Of Ceranib-2 Offering Potential For A New Antineoplastic Agent In The Treatment Of Cancer Cells, Djanan Vejselova, Hati̇ce Mehtap Kutlu, Gökhan Kuş, Selda Kabadere, Ruhi̇ Uyar
Turkish Journal of Biology
Fibrocarcinomas are malignant tumours that originate in mesenchymal cells. The tumours typically form in the presence of connective tissue and are of key consideration when assessing research priorities for future healthcare management. Ceramidase inhibitors, such as ceranib-2, have demonstrated capacity to interfere with cellular DNA functionality, initiating apoptosis in many cancer cell lines. The enzyme ceramidase can regulate cellular levels of sphingosine and sphingosine-1-phosphate by controlling hydrolysis of ceramide. The present study investigates the antigrowth effects of ceranib-2 on mouse embryonic fibroblast cells (NIH/3T3 normal cell line) and rat embryonic fibroblast cells (5RP7 cancer cell line). Research was conducted using …
Induction Of Apoptosis In The Cervical Cancer Cell Line Hela By A Novel Metabolite Extracted From The Fungus Aspergillus Japonicus Saito, Apoorva Prabhu, Prerana Venkat, Bharath Gajaraj, Varalakshmi Kilingar Nadumane
Induction Of Apoptosis In The Cervical Cancer Cell Line Hela By A Novel Metabolite Extracted From The Fungus Aspergillus Japonicus Saito, Apoorva Prabhu, Prerana Venkat, Bharath Gajaraj, Varalakshmi Kilingar Nadumane
Turkish Journal of Biology
Natural bioactive compounds have been found superior to chemical compounds for use in pharmaceutical applications. Such natural compounds include biologically active compounds present as a part of fungal metabolism that originate through different biochemical pathways and contain different structural groups like benzopyranones, phenols, toxins, and steroids. One such novel compound is a secondary metabolite extracted from the filamentous fungus Aspergillus japonicus Saito. The compound exhibited anticancer properties and was found to be harmless to normal human lymphocytes. The metabolites were separated by thin layer chromatography and screened for their cytotoxic potential through 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay in the cervical cancer …
Anticancer Activities And Mechanism Of Action Of 2 Novel Metal Complexes, C_{16}H_{34}N_{8}O_{5}Ag_{2}Cd And C_{11}H_{16}N_{7}O_{2}Ag_{3}Ni, Ali̇ Aydin, Nesri̇n Korkmaz, Şaban Teki̇n, Ahmet Karadağ
Anticancer Activities And Mechanism Of Action Of 2 Novel Metal Complexes, C_{16}H_{34}N_{8}O_{5}Ag_{2}Cd And C_{11}H_{16}N_{7}O_{2}Ag_{3}Ni, Ali̇ Aydin, Nesri̇n Korkmaz, Şaban Teki̇n, Ahmet Karadağ
Turkish Journal of Biology
The discovery of anticancer activity in cisplatin triggered the development of novel drugs containing metals such as platinum or ruthenium. Extremely diverse structural chemistry and the interaction of metal complexes with biomolecules resulted in the exploration of novel metal complexes with drug potential. In the present study, the anticancer and cytotoxic activities and the mechanisms of action were investigated for C_{16}H_{34}N_{8}O_{5}Ag_{2}Cd (AN1) and C_{11}H_{16}N_{7}O_{2}Ag_{3}Ni (AN7), 2 newly synthesized dicyanidoargentate(I) complexes. The anticancer and cytotoxic activities of AN1 and AN7 on several cancer cell lines were tested by cell proliferation and cytotoxic activity assays, respectively. The apoptotic and replication inhibitory potentials …
Plzf Overexpression In T-All Cell Line Cem-C7h2-2c8 Downregulates Glucocorticoid Receptor (Gr) And Its Target Genes, Muhammad Wasim, Muhammad Mansha, Muhammad Tayyab, Ali Raza Awan, Sehrish Firyal, Christine Mantinger, Tahir Yaqub
Plzf Overexpression In T-All Cell Line Cem-C7h2-2c8 Downregulates Glucocorticoid Receptor (Gr) And Its Target Genes, Muhammad Wasim, Muhammad Mansha, Muhammad Tayyab, Ali Raza Awan, Sehrish Firyal, Christine Mantinger, Tahir Yaqub
Turkish Journal of Biology
Glucocorticoids (GCs) induce cell cycle arrest and apoptosis in lymphoid cells and constitute a central component in the treatment of lymphoid malignancies. The molecular basis of this clinically important phenomenon remains, however, poorly understood. Using whole genome expression profiling we have previously identified glucocorticoid response genes in children with acute lymphoblastic leukemia (ALL). The promyelocytic leukemia zinc finger (PLZF) appeared as one of the most promising candidate genes, which has been implicated in the pathogenesis of several leukemia types. We have already established that transgenic PLZF reduced the sensitivity to GC-induced apoptosis in the CEM-C7H2-2C8 leukemic cell line and knockdown …
Photocatalytic Antimicrobial Effect Of Tio_2 Anatase Thin-Film-Coated Orthodontic Arch Wires On 3 Oral Pathogens, Fi̇gen Özyildiz, Ataç Uzel, Ayşe Serpi̇l Hazar, Mustafa Güden, Sultan Ölmez, Işil Aras, İsmai̇l Karaboz
Photocatalytic Antimicrobial Effect Of Tio_2 Anatase Thin-Film-Coated Orthodontic Arch Wires On 3 Oral Pathogens, Fi̇gen Özyildiz, Ataç Uzel, Ayşe Serpi̇l Hazar, Mustafa Güden, Sultan Ölmez, Işil Aras, İsmai̇l Karaboz
Turkish Journal of Biology
The aim of this study was to introduce antimicrobial activity to stainless steel orthodontic arch wires by coating them with TiO_2 in anatase form. Stainless steel (0.016 × 0.022 inch), D-rect (0.016 × 0.022 inch), and multistranded hammered retainer wires (0.014 × 0.018 inch) were coated with TiO_2 anatase by the sol-gel dip coating method. The wires were assessed for their photocatalytic antimicrobial activity against Streptococcus mutans, Candida albicans, and Enterococcus faecalis. After illumination under UVA (315-400 nm) at 1.0 mW/cm^2 for 1 h, the reduction efficiencies of the anatase-coated arch wires were calculated by using colony-forming unit counts. All …
Activity Of Antioxidant Enzymes During Induction Of Morphogenesis Of Fritillaria Meleagris In Bulb Scale Culture, Marija Petric, Sladana Jevremovic, Milana Trifunovic, Vojin Tadic, Snezana Milosevic, Angelina Subotic
Activity Of Antioxidant Enzymes During Induction Of Morphogenesis Of Fritillaria Meleagris In Bulb Scale Culture, Marija Petric, Sladana Jevremovic, Milana Trifunovic, Vojin Tadic, Snezana Milosevic, Angelina Subotic
Turkish Journal of Biology
We investigated the activity of 3 antioxidant enzymes: superoxide dismutase, catalase, and peroxidase during morphogenesis of Fritillaria meleagris L. in vitro in bulb scale culture. Bulb cultures of F. meleagris were established on nutrition medium supplemented with 1.0 mg L^{-1} thidiazuron. Bulbs were grown at standard (24 °C) and low temperatures (4 °C) or at an increased sucrose concentration (4.5%) during the first 6 weeks (pretreatments). All bulbs were then grown for 4 weeks at standard temperature on 2, 4-dichlorophenoxyacetic acid and kinetin or thidiazuron (at concentrations of 1 mg L^{-1} each) in order to examine the influence of the …