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Articles 571 - 600 of 1681
Full-Text Articles in Biology
Amplification Of Gc-Rich Adamts-2 And Urg4/Urgcp Promoter Regions With Optimized Combination Of Pcr Enhancers, Meltem Alper, Esra Tokay, Feray Köçkar
Amplification Of Gc-Rich Adamts-2 And Urg4/Urgcp Promoter Regions With Optimized Combination Of Pcr Enhancers, Meltem Alper, Esra Tokay, Feray Köçkar
Turkish Journal of Biology
PCR is an effective and widely used method for the amplification of target DNA fragments in vitro. The templates that have high guanine-cytosine (GC) content, complex secondary structures, or short tandem repeats are difficult to amplify by the conventional PCR methods. Human genome regulatory regions such as promoters are rich in terms of GC base compositions. To amplify these regions some special modifications or additives are required. Using different PCR strategies such as hot-start/touchdown PCR or the utilization of various additives into the reaction mixture such as organic molecules can improve PCR yield. In the present study we optimized the …
Cloning And Functional Characterization Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Daiwen Chen, Bing Yu, Junqiu Luo, Zhiqing Huang
Cloning And Functional Characterization Of Porcine Sox6, Wanxue Wen, Xiaoling Chen, Daiwen Chen, Bing Yu, Junqiu Luo, Zhiqing Huang
Turkish Journal of Biology
Sox6, a fast myofiber-enriched repressor of slow muscle gene expression, plays an important role in the control of skeletal muscle fiber-type transition. Mammalian Sox6 has been isolated and characterized from mice, rats, and humans. In this study, the porcine Sox6 (pSox6) cDNA was cloned by degenerate PCR. The entire open reading frame (ORF) of pSox6 is 2406 bp. The predicted protein is composed of 801 amino acids and contains the HMG box, leucine-zipper motif, and glutamine-rich Q-box. The amino acid similarities between the Sox6 from pig and other mammals range from 90% to 95%. Real-time quantitative PCR analysis indicated that …
Identification And Characterisation Of Mlo Genes In Pea (Pisum Sativum L.)Vis-À-Vis Validation Of Mlo Gene-Specific Markers, Chinmayee Mohapatra, Ramesh Chand, Vinay Kumar Singh, Anil Kumar Singh, Chanda Kushwaha
Identification And Characterisation Of Mlo Genes In Pea (Pisum Sativum L.)Vis-À-Vis Validation Of Mlo Gene-Specific Markers, Chinmayee Mohapatra, Ramesh Chand, Vinay Kumar Singh, Anil Kumar Singh, Chanda Kushwaha
Turkish Journal of Biology
Pea is infected by a number of pathogens, and among them powdery mildew (PM) caused by Erysiphe pisi is an important fungal disease. This work was undertaken in silico to characterise the identified PM resistant genes of pea and design specific primers to be used in marker-assisted selection (MAS) of resistant and susceptible pea genotypes. Mlo gene sequences of A. thaliana and O. sativa were retrieved from different databases and used for a homology search of P. sativum EST databases. Each identified gene was used for a similarity search and phylogenetic classification with different crops. Four gene-specific primers were designed …
Production Of Recombinant Human Dipeptidyl Peptidase Iv From Sf9cells In Microbial Fermenters, Özlem Ayteki̇n, Sai̇me İsmet Gürhan, Kayoko Ohura, Teruko Imai, Gaye Öngen
Production Of Recombinant Human Dipeptidyl Peptidase Iv From Sf9cells In Microbial Fermenters, Özlem Ayteki̇n, Sai̇me İsmet Gürhan, Kayoko Ohura, Teruko Imai, Gaye Öngen
Turkish Journal of Biology
The human dipeptidyl peptidase IV (hDPPIV/CD26) is expressed as an immune response in some cancer cells as well as intestine and incretin metabolism, and deficiency of the enzyme leads to metabolic disorders. In the present study, recombinant hDPPIV/CD26 genes were expressed in baculovirus-insect cell systems in a 5-L stirred-tank fermenter. Because of the shear sensitivity of the insect cell line, production from insect cells should be performed in new-generation type bioreactors, which are commonly more expensive than microbial fermenters. To optimize the process, hydrodynamic parameters and oxygen consumption of Sf9 cells at 1.5 L and 3 L were monitored, and …
Histopathological And Biochemical Changes In The Liver, Kidney, And Bloodof Amphibians Intoxicated With Cadmium, Marcela Fátima Medina, Maria Elina González, Sandra Maria Roxana Klyver, Iris Maria Aybar Odstrcil
Histopathological And Biochemical Changes In The Liver, Kidney, And Bloodof Amphibians Intoxicated With Cadmium, Marcela Fátima Medina, Maria Elina González, Sandra Maria Roxana Klyver, Iris Maria Aybar Odstrcil
Turkish Journal of Biology
The worldwide decline in amphibian populations has made heavy metal pollution a subject of interest. The aim of this work was to analyze the effect of sublethal doses of cadmium (Cd) on the liver, kidney, and blood of Rhinella arenarum specimens. Serum markers indicative of hepatic injury (gamma glutamyltransferase, alkaline phosphatase, pseudo cholinesterase, and total cholesterol) and markers of renal dysfunction (blood urea nitrogen, serum creatinine, calcium, and glucose) showed variation in animals treated with a 0.5 mg/kg dose compared to controls. Histopathological images revealed alterations in the liver (hepatocyte ballooning and hyperplasia of Kupffer cells) and kidney (renal tubular …
Phylogenetic Relationships Between Oxytropis Dc. And Astragalus L.Species Native To An Old World Diversity Center Inferred From Nuclear Ribosomal Its And Plastid Matk Gene Sequences, Ayten Tekpinar, Seher Karaman Erkul, Zeki̇ Aytaç, Zeki̇ Kaya
Phylogenetic Relationships Between Oxytropis Dc. And Astragalus L.Species Native To An Old World Diversity Center Inferred From Nuclear Ribosomal Its And Plastid Matk Gene Sequences, Ayten Tekpinar, Seher Karaman Erkul, Zeki̇ Aytaç, Zeki̇ Kaya
Turkish Journal of Biology
Oxytropis and Astragalus represent one of the largest angiosperm genera complexes. Although phylogenetic studies of this complex exist, the evolutionary relationships among Astragalus and Oxytropis species sharing similar habitats in the Old World have not been studied in detail. The phylogenetic relationships among 13 Oxytropis and 56 Astragalus species native to Turkey were inferred from nucleotide sequence variations in the nuclear ribosomal internal transcribed spacer (ITS) and chloroplast maturase-like protein (matK) gene regions. In addition to our samples, 36 Oxytropis ITS and 6 Oxytropis matK sequences were retrieved from GenBank and included in the analysis. Phylogenies derived from a maximum …
Identification Of Small Molecule Binding Pocket For Inhibition Of Crimean?Congo Hemorrhagic Fever Virus Otu Protease, Fati̇h Kocabaş, Enes Kemal Ergi̇n
Identification Of Small Molecule Binding Pocket For Inhibition Of Crimean?Congo Hemorrhagic Fever Virus Otu Protease, Fati̇h Kocabaş, Enes Kemal Ergi̇n
Turkish Journal of Biology
Crimean-Congo hemorrhagic fever virus (CCHFV) is a deadly tick-borne virus with high mortality rates. Current antivirals lack specificity, making them susceptible to off-target effects and cytotoxicity. There is an utmost need for the identification of active compounds for anti-CCHFV therapies. Inhibition of CCHFV ovarian tumor (OTU) protease by small molecules is an exciting potential antiviral therapy. In this study, computational approaches based on residue homology, the binding coordinates of ligands, and correlation analysis with in vitro data identified the pocket of Y89-W99 as the inhibition site of CCHFV OTU protease. In silico screening of more than 600,000 compounds against this …
Effects Of Amniotic Fluid Mesenchymal Stem Cells In Carboxymethyl Cellulosegel On Healing Of Spontaneous Pressure Sores: Clinical Outcome In Sevenhospitalized Neonatal Foals, Eleonora Iacono, Aliai Lanci, Barbara Merlo, Francesca Ricci, Alessandro Pirrone, Carlotta Antonelli, Jole Mariella, Carolina Castagnetti
Effects Of Amniotic Fluid Mesenchymal Stem Cells In Carboxymethyl Cellulosegel On Healing Of Spontaneous Pressure Sores: Clinical Outcome In Sevenhospitalized Neonatal Foals, Eleonora Iacono, Aliai Lanci, Barbara Merlo, Francesca Ricci, Alessandro Pirrone, Carlotta Antonelli, Jole Mariella, Carolina Castagnetti
Turkish Journal of Biology
In the present study, an innovative therapy for spontaneously arisen pressure sores in seven hospitalized neonatal foals is described, using mesenchymal stem cells obtained from equine amniotic fluid. Amniotic fluid mesenchymal stem cells (AFMSCs) were isolated from fluid samples recovered at delivery. Heterologous cells, at passage three of in vitro culture, were applied to sores twice a week for four consecutive times in a carboxymethylcellulose (CMC) gel. As a control, a commercial ointment was used. The results showed that the mean sore regression rate with AFMSCs in CMC gel was statistically higher than the mean value recorded in the control …
Direct Shoot Organogenesis And Agrobacterium Tumefaciens Mediated Transformation Of Solanum Trilobatum L., Jayabalan Shilpha, Manoharan Jayashre, Muthiah Joe Virgin Largia, Manikandan Ramesh
Direct Shoot Organogenesis And Agrobacterium Tumefaciens Mediated Transformation Of Solanum Trilobatum L., Jayabalan Shilpha, Manoharan Jayashre, Muthiah Joe Virgin Largia, Manikandan Ramesh
Turkish Journal of Biology
Solanum trilobatum L. İs one of the most valued medicinal plants of Indian Ayurveda and Siddha medicine. An efficient protocol for direct shoot organogenesis and Agrobacterium tumefaciens mediated transformation using in vitro derived leaf explants of S. Trilobatum was established for the first time. High frequency direct shoot regeneration (89%) was achieved in MS medium containing 2 mg/L 6-benzylaminopurine and 0.25 mg/L indole-3-acetic acid. The Agrobacterium strain EHA 105 harboring the binary vector pCAMBIA 1301, which contained hygromycin phosphotransferase (hpt) as a selectable marker gene and β-glucuronidase (gus) as a reporter gene, was used for the transformation. The factors that …
Biochemical Characterization Of Wild-Type And Mutant (Q9f And S21y/V22d)Iron Oxidases Isolated From Acidithiobacillus Ferrooxidans M1, Di̇lşat Ni̇gar Çolak, Hali̇l İbrahi̇m Güler, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz
Biochemical Characterization Of Wild-Type And Mutant (Q9f And S21y/V22d)Iron Oxidases Isolated From Acidithiobacillus Ferrooxidans M1, Di̇lşat Ni̇gar Çolak, Hali̇l İbrahi̇m Güler, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz
Turkish Journal of Biology
Iron oxidase, a member of the high potential iron-sulfur protein (HiPIP) family within the iron-sulfur cluster, was thought to be involved in the iron respiratory electron transport chain in Acidithiobacillus ferrooxidans. A. ferrooxidans M1 strain was isolated from Murgul copper mine. The iro gene of this bacterium encoding iron oxidase was cloned, and the complete nucleotide sequence was disclosed. The gene was cloned and overexpressed successfully. The highly conserved amino acid residues within the iron oxidase enzyme sequence were determined, and their Q9F and S21Y/V22D recombinants were created through site-directed mutagenesis. Wild-type and recombinant iron oxidase enzymes were purified and …
The Aggregatibacter Actinomycetemcomitans Heat Shock Protein Groel Interacts Directly With Human Peripheral Blood T Cells, Ayten Nalbant, Tahsi̇n Saygili
The Aggregatibacter Actinomycetemcomitans Heat Shock Protein Groel Interacts Directly With Human Peripheral Blood T Cells, Ayten Nalbant, Tahsi̇n Saygili
Turkish Journal of Biology
Heat shock family protein GroEL of Aggregatibacter actinomycetemcomitans (Aa) has antigenic properties. We previously demonstrated that A. actinomycetemcomitans GroEL-like protein affects human CD4 T cells by converting them into IL-10 and IFNg double cytokine producing Tbet+ Th1 cells. The objective of this study was to investigate whether or not AaGroEL communicates with T cells directly. To do this, sorted cells from peripheral blood mononuclear cells were stimulated with AaGroEL for 48 h. Flow cytometry was used to measure soluble and intracellular cytokine expression in the cell cultures and detect TLR2 expression on the surface of T cells. Expression of six …
Identification And Sequence Analysis Of Alkaloid Biosynthesisgenes In Papaver Section Oxytona, Yağiz Alagöz, Tuğba Gürkök, İskender Parmaksiz, Turgay Ünver
Identification And Sequence Analysis Of Alkaloid Biosynthesisgenes In Papaver Section Oxytona, Yağiz Alagöz, Tuğba Gürkök, İskender Parmaksiz, Turgay Ünver
Turkish Journal of Biology
The benzylisoquinoline alkaloids (BIA) are secondary metabolites that are produced by many of the Papaver species including Papaver bracteatum, Papaver pseudo-orientale, and Papaver orientale, three representative members of the section Oxytona Bernh. The sequences and expression levels of the genes positioned on the BIA biosynthesis pathway were previously defined in opium poppy (Papaver somniferum), one of the mostly studied species of the same genus. Nevertheless, the majority of predicted BIA-related gene sequences in the section Oxytona have not been specified. Here we are presenting new cDNA sequences that belong to the Oxytona species. In this study, partial sequences of norcoclaurine-6-O-methyltransferase …
Chondroitin Sulfate-Based Biomaterials For Tissue Engineering, Hyuck Joon Kwon, Youngbae Han
Chondroitin Sulfate-Based Biomaterials For Tissue Engineering, Hyuck Joon Kwon, Youngbae Han
Turkish Journal of Biology
Chondroitin sulfate (CS) is a sulfated glycosaminoglycan (GAG) that is usually found attached to proteins as part of a proteoglycan. The use of CS-based biomaterials in the field of tissue engineering applications has been intensively growing over the past decades because CS is a biopolymer with the major advantages of being biodegradable, biocompatible, easily available, and highly versatile. In vitro and in vivo studies have shown that CS-based biomaterials upregulate cartilage-specific gene expression in chondrocytes and mesenchymal stem cells and also support osteogenic differentiation by increasing the effectiveness of bone anabolic growth factors. Chondroitin sulfate proteoglycan plays a key role …
Antisenescence Activity Of G9a Inhibitor Bix01294 On Human Bone Marrow Mesenchymal Stromal Cells, Min-Ji Ahn, Sin-Gu Jeong, Goang Won Cho
Antisenescence Activity Of G9a Inhibitor Bix01294 On Human Bone Marrow Mesenchymal Stromal Cells, Min-Ji Ahn, Sin-Gu Jeong, Goang Won Cho
Turkish Journal of Biology
Alterations in epigenomic patterns are associated with diverse physiological functions and pathological mechanisms causing aging-related diseases, such as degenerative disorders and cancers. We investigated the senescent effects of BIX01294, a G9a (histone methyltransferase) inhibitor, in human bone marrow mesenchymal stromal cells (hBM-MSCs). We determined the optimal dose and time of BIX01294 treatment in hBM-MSCs to be 1 μM and 12 h, respectively. Under these conditions, the expression of the antisenescent genes TERT, bFGF, VEGF, and Oct-4 increased, whereas the expression of the senescent factors p16, p21, and p53 decreased. The number of β-galactosidase-positive cells decreased significantly, and the rates of …
Rejuvenation Of Mature Lentisk By Micrografting And Evaluation Of Genetic Stability, Ahmet Onay, Engi̇n Ti̇lkat, Veysel Süzerer, Özge Karakaş Meti̇n, Yelda Özden Çi̇ftçi̇, Fati̇h Mehmet Kilinç, İbrahi̇m Koç, Muhammet Şaki̇roğlu, Hakan Yildirim, Ahu Altinkut Uncuoğlu, Nazan Çalar, Ömer Faruk Akdemi̇r
Rejuvenation Of Mature Lentisk By Micrografting And Evaluation Of Genetic Stability, Ahmet Onay, Engi̇n Ti̇lkat, Veysel Süzerer, Özge Karakaş Meti̇n, Yelda Özden Çi̇ftçi̇, Fati̇h Mehmet Kilinç, İbrahi̇m Koç, Muhammet Şaki̇roğlu, Hakan Yildirim, Ahu Altinkut Uncuoğlu, Nazan Çalar, Ömer Faruk Akdemi̇r
Turkish Journal of Biology
An in vitro propagation method was established for both male and female genotypes of lentisk using actively growing shoot tips derived from forcefully lignified shoots. The effects of growth regulators on in vitro morphogenesis were investigated. Since rooting of the regenerated shoots for both genotypes was not achieved, an in vitro micrografting method was developed for the production of plantlets. Moreover, genetic stability of 3-, 6-, and 24-times subcultured clones of both genotypes was assessed and compared with the mother plants using fluorescent-based amplified fragment length polymorphism (AFLP) analysis. The set of main plants and the different subcultured clones were …
Cloning, Expression, And In Silico Analysis Of A Novel Annexin Gene Ftanx1from Tartary Buckwheat (Fagopyrum Tataricum (L.) Gaertn.), Lingli Hou, Xueni Dong, Mengqi Ding, Xuemei Zhu, Jirong Shao
Cloning, Expression, And In Silico Analysis Of A Novel Annexin Gene Ftanx1from Tartary Buckwheat (Fagopyrum Tataricum (L.) Gaertn.), Lingli Hou, Xueni Dong, Mengqi Ding, Xuemei Zhu, Jirong Shao
Turkish Journal of Biology
Annexins are generally thought to play an essential role in the regulation of plant growth, development, and stress responses. Here we describe an annexin gene from Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.), designated as FtANX1. The full-length coding sequence of FtANX1 is 942 bp in size, encoding a polypeptide of 313 amino acids with a theoretical molecular mass of 36.0 kDa and pI 5.94. In silico analysis of the sequence revealed that FtANX1 contains four repeats, with a type-II Ca2+-binding site in repeat I. Expression pattern analysis provided evidence that FtANX1 was predominantly expressed in immature achenes, while its level …
Apoptotic Induction Via Membrane/Dna Damage And Metabolic Inactivation By Synthetic Food Colorants In Allium Cepa Root Meristem, Prajitha Vazhangat, John Ernest Thoppil
Apoptotic Induction Via Membrane/Dna Damage And Metabolic Inactivation By Synthetic Food Colorants In Allium Cepa Root Meristem, Prajitha Vazhangat, John Ernest Thoppil
Turkish Journal of Biology
The present study investigates the cytotoxic and genotoxic effects of two frequently employed synthetic food colorants, lemon yellow and orange red, using an Allium cepa assay. Uptake of food colorants by root cells at different concentrations and varied exposures significantly altered the cytogenetic system, membrane integrity, mitochondrial function, root growth, and cell division. The food colorants induced significant DNA damage, micronucleus formation, and other severe chromosomal aberrations at genetic level and inhibited cell division as well as root growth. Severe DNA damage, membrane damage, and metabolic inactivation observed in the result are indicative of apoptotic cell death. These results confirm …
Efficient Seed-Specifically Regulated Autoexcision Of Marker Gene (Nptii) With Inducible Expression Of Interest Gene In Transgenic Nicotiana Tabacum, Shiva Hamzeh, Mostafa Motallebi, Mohammad Reza Zamani
Efficient Seed-Specifically Regulated Autoexcision Of Marker Gene (Nptii) With Inducible Expression Of Interest Gene In Transgenic Nicotiana Tabacum, Shiva Hamzeh, Mostafa Motallebi, Mohammad Reza Zamani
Turkish Journal of Biology
In this study, we developed the seed-specifically regulated autoexcision system based on Cre/loxP site-specific recombination for coelimination of nptII and cre genes from transgenic plants. To accomplish this, a seed-specific gene promoter, BcNA1, from Brassica napus was used to drive conditional expression of recombinase. NptII and recombinase cassettes were placed between two directly repeated loxP sites. The loxP-flanked DNA was located between the SP-DDEE synthetic pathogen inducible promoter and pr??omoterless β-glucuronidase (Gus) gene, as a model gene of interest. Upon seed-specific expression of cre recombinase, the excision event would eliminate loxP-flanked DNA and bring the gus reporter gene under the …
Mucus Of Achatina Fulica Stimulates Mineralization And Inflammatoryresponse In Dental Pulp Cells, Fahsai Kantawong, Pichaporn Thaweenan, Sutinee Mungkala, Sawinee Tamang, Ruthairat Manaphan, Phenphichar Wanachantararak, Teerasak E-Kobon, Pramote Chumnanpuen
Mucus Of Achatina Fulica Stimulates Mineralization And Inflammatoryresponse In Dental Pulp Cells, Fahsai Kantawong, Pichaporn Thaweenan, Sutinee Mungkala, Sawinee Tamang, Ruthairat Manaphan, Phenphichar Wanachantararak, Teerasak E-Kobon, Pramote Chumnanpuen
Turkish Journal of Biology
Dental pulp tissue contains stem cells that can be isolated and used for regenerative medicine in tooth restoration or autologous transplantation. The aim of this study was to observe the mineralization and gene expression in dental pulp cells (DPCs) following treatment with snail mucus. Snail mucus was collected from adult Achatina fulica and processed as a dry powder by the freeze-drying technique. The mucus powder was dissolved in a culture medium at a concentration of 15 μg/mL. DPCs were prepared by the outgrowth technique and cultured in a 6-well plate at a density of 5 × 104 cells per well. …
Enhancement Of Chondrogenic Differentiation Potential Of Equine Adiposetissue-Derived Mesenchymal Stem Cells Using Tgf-Ss3 And Bmp-6, Milad Shademan, Abbas Parham, Hesam Dehghani
Enhancement Of Chondrogenic Differentiation Potential Of Equine Adiposetissue-Derived Mesenchymal Stem Cells Using Tgf-Ss3 And Bmp-6, Milad Shademan, Abbas Parham, Hesam Dehghani
Turkish Journal of Biology
This study was designed to evaluate the in vitro chondrogenic differentiation potential of equine adipose tissue-derived mesenchymal stem cells (AT-MSCs) in response to different culture conditions. Fat tissue cells after collection were cultured in optimized conditions until passage 3 (P3). P3 cells were cultured as micropellets under different chondrogenic conditions for 21 days in 5 groups: basic medium as a control, basic chondrogenic medium (BCM), BCM supplemented with transforming growth factor beta 3 (TGF-β3; 10 ng/mL), BCM supplemented with bone morphogenetic protein 6 (BMP-6; 10 ng/mL), and BCM supplemented with both TGF-β3 and BMP-6. The growth rate of pellets was …
Construction Of An Oxygen Detection-Based Optic Laccase Biosensor For Polyphenolic Compound Detection, Kadi̇r Bi̇li̇r, Marie-Theres Weil, Julia Lochead, Fatma Neşe Kök, Tobias Werner
Construction Of An Oxygen Detection-Based Optic Laccase Biosensor For Polyphenolic Compound Detection, Kadi̇r Bi̇li̇r, Marie-Theres Weil, Julia Lochead, Fatma Neşe Kök, Tobias Werner
Turkish Journal of Biology
A fiber optic biosensor was constructed from Pleurotus ostreatus laccase for the detection of polyphenolic compounds. Laccase was immobilized on the surface of the commercially available fiber optic oxygen sensor spots by using 3-aminopropylsilanetriol, glutaraldehyde, and amino-modified carboxycellulose. A diffusion layer containing tetramethyl orthosilicate (TMOS), trimethoxymethylsilane (Tri-MOS), and polyvinyl alcohol (PVA) was added to the immobilized laccase layer. The consumption of oxygen as a result of laccase activity was monitored using a fiber optical measuring setup with catechol as a model substrate. The optimal enzyme amount was determined as 1.5 mg per 50 µL of enzyme layer mixture, and with …
Higher Expression Level Of Bat3 Is Associated With Silencing Of Themidn Gene In Primary Mouse Cardiomyocytes, Bi̇lge Şadan Selçuk, Ayşe Evri̇m Bayrak, Ni̇han Ünaltuna
Higher Expression Level Of Bat3 Is Associated With Silencing Of Themidn Gene In Primary Mouse Cardiomyocytes, Bi̇lge Şadan Selçuk, Ayşe Evri̇m Bayrak, Ni̇han Ünaltuna
Turkish Journal of Biology
Midnolin (Midn) is a developmentally regulated nucleolar protein that was originally identified in the mesencephalon of developing mouse embryos. Additionally, Midn expression was detected in various adult tissues and was associated with glucokinase activity in pancreatic beta cells and with the regulation of folliculogenesis in ovaries. In this study, our aim was to analyze the expression and function of the Midn gene in cardiomyocyte cells. We constructed a primary neonatal mouse cardiomyocyte cell culture and analyzed the expression of possible Midn-associated genes after short interfering RNA (siRNA)-mediated gene silencing for Midn. After 24 h of silencing, we observed that the …
Genome-Wide Analysis Of Response To Low Sulfur (Lsu) Genes In Grass Species And Expression Profiling Of Model Grass Species Brachypodium Distachyon Under S Deficiency, Hüseyi̇n Tombuloğlu, Abdugaffor Ablazov, Ertuğrul Fi̇li̇z
Genome-Wide Analysis Of Response To Low Sulfur (Lsu) Genes In Grass Species And Expression Profiling Of Model Grass Species Brachypodium Distachyon Under S Deficiency, Hüseyi̇n Tombuloğlu, Abdugaffor Ablazov, Ertuğrul Fi̇li̇z
Turkish Journal of Biology
Sulfur (S) affects the plant life cycle and crop yield and has nutritional importance for human and animal diet. Its deficiency is one of the major problems in agriculture. However, the plant-specific LSU (response to Low Sulfur) gene family has not been extensively analyzed in major plant species such as grasses. In this study, we have performed in silico genome-wide analysis of LSU genes in 6 grass species, including Brachypodium distachyon, Sorghum bicolor, Oryza sativa, Zea mays, Triticum aestivum, and Panicum virgatum. All identified LSU genes contained one exon encoding proteins of acidic character with cytoplasmic localization. In silico analysis …
Cardiac Patch Design: Compatibility Of Nanofiber Materials Prepared Byelectrospinning Method With Stem Cells, Betül Çelebi̇ Saltik, Mustafa Özgür Öteyaka
Cardiac Patch Design: Compatibility Of Nanofiber Materials Prepared Byelectrospinning Method With Stem Cells, Betül Çelebi̇ Saltik, Mustafa Özgür Öteyaka
Turkish Journal of Biology
In this article, in order to create a cardiac patch, a biocompatible polyurethane (PU) nanofiber polymer was developed with the electrospinning method. Bone marrow mesenchymal stem cells (BM MSCs) were isolated from whole human bone marrow. Isolated BM MSC homogeneity was determined by flow cytometry using specific markers. BM MSC-PU interactions were studied with a WST-1 kit on the 3rd and 7th days. Cardiomyocyte differentiation was performed with 5-azacytidine. Cell survival and proliferation of cardiomyocyte-like cells that were cultured on nerve growth factor (NGF)-embedded PU were evaluated with the WST-1 kit on the 3rd and 7th days. NGF's effect on …
Cloning Of Two Basic Helix-Loop-Helix Transcription Factor Genes From Tartary Buckwheat (Fagopyrum Tataricum) And Their Expression Under Abiotic Stress, Xiao-Peng Luo, Hai-Xia Zhao, Jun Xue, Cheng-Lei Li, Hui Chen, Sang-Un Park, Qi Wu
Cloning Of Two Basic Helix-Loop-Helix Transcription Factor Genes From Tartary Buckwheat (Fagopyrum Tataricum) And Their Expression Under Abiotic Stress, Xiao-Peng Luo, Hai-Xia Zhao, Jun Xue, Cheng-Lei Li, Hui Chen, Sang-Un Park, Qi Wu
Turkish Journal of Biology
Basic helix-loop-helix (bHLH) transcription factors are one of the most important transcription factors involved in abiotic stress tolerance in Tartary buckwheat. In this study, we successfully isolated two bHLH transcription factor genes, designated as FtbHLH1 and FtbHLH2, from Tartary buckwheat. Their encoding proteins were found to have a motif to interact with R2R3-MYB at the N-terminus and a typical bHLH motif at the C-terminus. Yeast one-hybrid assay proved that the two bHLH proteins exhibited obvious transcriptional activation activities. The abiotic stresses indicated that FtbHLH1 expression would be mainly affected by drought and UV-B, whereas FtbHLH2 expression would present a sensitive …
Enhanced Salt Tolerance Of Transgenic Tobacco Expressing A Wheat Salt Tolerance Gene, Musa Kavas, Mehmet Cengi̇z Baloğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Enhanced Salt Tolerance Of Transgenic Tobacco Expressing A Wheat Salt Tolerance Gene, Musa Kavas, Mehmet Cengi̇z Baloğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Soil salinity is one of the most important limiting factors of agricultural productivity in the world. The Triticum aestivum salt tolerance-related gene (TaSTRG) possesses a functional response to salt and drought stress conditions. A variety of stress factors, such as salt, drought, abscisic acid, and cold, may induce the expression of TaSTRG in wheat. In this study, the TaSTRG gene was transferred to tobacco via Agrobacterium-mediated transformation. Overexpression of TaSTRG in transgenic tobacco plants indicated higher salt tolerance and mediated more vigorous growth than in wild-type plants. Under salt stress conditions, the transgenic tobacco plants had higher germination and survival …
Role Of Glucose Repression In The Oxidative Stress Response Ofschizosaccharomyces Pombe: Analysis Of Transcript Levels Of Fbp1, Hxk2, Sod1and Ctt1 Genes In Sty1, Atf1 And Pap1 Knock-Out Mutants, Bedi̇a Palabiyik, Farinaz Jafari Ghods, Semi̇an Karaer Uzuner
Role Of Glucose Repression In The Oxidative Stress Response Ofschizosaccharomyces Pombe: Analysis Of Transcript Levels Of Fbp1, Hxk2, Sod1and Ctt1 Genes In Sty1, Atf1 And Pap1 Knock-Out Mutants, Bedi̇a Palabiyik, Farinaz Jafari Ghods, Semi̇an Karaer Uzuner
Turkish Journal of Biology
Previously we reported that the glucose sensing/signaling pathway affected the oxidative stress response of Schizosaccharomyces pombe, based on studies of glucose-repression and oxidative-stress-resistant mutants including ird5. sty1, encoding a protein kinase, and aft1 and pap1, encoding transcription factors, are important components of the oxidative stress response in S. Pombe. To analyze the relationship between the glucose sensing/signaling pathway and the oxidative stress response, we generated sty1, aft1, and pap1 knock-out mutants in ird5 and wild-type backgrounds. We evaluated the survival rates of the ird5 double mutants (5A, 5P, and 5S) and wild-type single mutants (9A, 9P, and 9S) under mild …
Stimulation With A 130-Mt Magnetic Field Improves Growth And Biochemical Parameters In Lupin (Lupinus Angustifolius L.), Magdalena Mroczek Zdyrska, Krzysztof Kornarzynski, Stanislaw Pietruszewski, Mariusz Gagos
Stimulation With A 130-Mt Magnetic Field Improves Growth And Biochemical Parameters In Lupin (Lupinus Angustifolius L.), Magdalena Mroczek Zdyrska, Krzysztof Kornarzynski, Stanislaw Pietruszewski, Mariusz Gagos
Turkish Journal of Biology
The influence of magnetic field stimulation (MFS) on plants is a subject of intense research. The influence of MFS on plants varies depending on its intensity, time of exposure, and form of application. Weak MFS has beneficial effects on physiological and biochemical processes in plant tissues. Lupins (Lupinus spp.) are economically and agriculturally important plants used mainly in livestock feeding or in human consumption. The effects of a stationary magnetic field (130 mT) on the mitotic activity and selected biochemical parameters of lupin (Lupinus angustifolius L.) were evaluated. Nonexposed plants were used as the control. It was noted that the …
Molecular Cytogenetic Identification Of A Novel Hexaploid Wheat-Thinopyrum Intermedium Partial Amphiploid With High Protein Content, Mariyana Georgieva, Klaudia Kruppa, Nedyalka Tyankova, Marta Molnar Lang
Molecular Cytogenetic Identification Of A Novel Hexaploid Wheat-Thinopyrum Intermedium Partial Amphiploid With High Protein Content, Mariyana Georgieva, Klaudia Kruppa, Nedyalka Tyankova, Marta Molnar Lang
Turkish Journal of Biology
Genomic in situ hybridization and fluorescence in situ hybridization were used to define the cytogenetic constitution of the intergeneric introgression line 29(1-57) obtained from the wheat × Thinopyrum intermedium (wheatgrass) cross. This line is a high-protein line resistant to leaf rust, yellow rust, and powdery mildew under field conditions and has 42 chromosomes. GISH analysis using a total genomic DNA probe from Thinopyrum intermedium resulted in the identification of 14 chromosomes of wheatgrass origin. The sequential GISH patterns obtained using St-genomic and J-genomic DNA as probes and ABD-genomic DNA from wheat as a blocker led to a better determination of …
Induction And Development Of Anther-Derived Gametic Embryos In Cucumber (Cucumis Sativus L.) By Optimizing The Macronutrient And Agar Concentrations In Culture Medium, Mohammad Reza Abdollahi, Samereh Najafi, Hasan Sarikhani, Sayyed Saeed Moosavi
Induction And Development Of Anther-Derived Gametic Embryos In Cucumber (Cucumis Sativus L.) By Optimizing The Macronutrient And Agar Concentrations In Culture Medium, Mohammad Reza Abdollahi, Samereh Najafi, Hasan Sarikhani, Sayyed Saeed Moosavi
Turkish Journal of Biology
Anther culture response of a cucumber cultivar (Beta Alpha) and three Iranian landraces of cucumber (Basmenj, Isfahani, and Korki) on MS culture media with an altered macronutrient strength (half, full, 1.5-fold, and double-strength) or various concentrations of agar (0, 3.5, 7, and 14 g L-1) was evaluated in two separate experiments. The effect of different concentrations of agar on the development of callus-derived embryos was also assessed. The androgenic potentials of various cucumbers were significantly (P < 0.01) influenced by the different macronutrient strengths and agar concentrations, although anther culture responses could vary according to the cucumber type. A doubled strength of macronutrients in culture medium resulted in the highest percentage of callogenesis (100%) in Isfahani cucumber, while use of full strength of macronutrients produced the highest percentage of embryogenic calli (83.33%) in the mentioned landrace compared to other tested forms. The maximum number of gametic embryos (1.26 and 1.23 embryos/anther) differentiated from Isfahani anther cultures on full- and half-strength MS medium, respectively. In the second experiment, the highest percentages of callogenesis were obtained in MS culture media solidified with 14 g L-1 and 7 g L-1 agar for Basmenj, with 3.5 g L-1 agar or liquid medium for Isfahani, with 7 g L-1 agar for Beta Alpha, and with 14 g L-1 agar for Korki. Culture media solidified with 7 g L-1 agar produced the highest percentage of embryogenic calli compared to other treatments. Anther culture of Korki cucumber on culture medium with 14 g L-1 agar produced the maximum number of embryos per anther compared to other cucumbers. Improved production of mature somatic embryos (torpedo and cotyledonary embryos) was achieved with higher agar concentrations (7 and 14 g L-1) for the cucumber cultivar and all landraces.