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Articles 331 - 360 of 1681
Full-Text Articles in Biology
Contribution Of Hepatitis B Virus X Protein-Induced Aberrant Microrna Expression To Hepatocellular Carcinoma Pathogenesis, Zhiyuan Wei, Xiaohe Shen, Bing Ni, Gaoxing Luo, Yi Tian, Yi Sun
Contribution Of Hepatitis B Virus X Protein-Induced Aberrant Microrna Expression To Hepatocellular Carcinoma Pathogenesis, Zhiyuan Wei, Xiaohe Shen, Bing Ni, Gaoxing Luo, Yi Tian, Yi Sun
Turkish Journal of Biology
The hepatitis B virus-encoded X (HBX) protein plays important roles in Hepatocellular carcinoma (HCC). Previous studies have demonstrated that HBX can induce alterations in the expression of numerous microRNAs (miRNAs) involved in the carcinogenesis of various tumors. However, the global profile of liver miRNA changes induced by HBX has not been characterized. In this study, we conducted a miRNA microarray analysis to investigate the influence of HBX on the expression of total miRNAs in liver in relation to HCC. Comparative analysis of the data from human normal liver cells (L02) and human HCC cells (HepG2), with or without HBX, identified …
Identification Of Bioactive Compounds From Fraxinus Angustifolia Extracts With Anti-Nadh Oxidase Activity Of Bovine Milk Xanthine Oxidoreductase, Nadjia Ahmane, Dina Atmani-Kilani, Nassima Chaher, Karima Ayouni, Meriem Rahmani-Berboucha, Gregory Da Costa, Nadjet Debbache-Benaida, Tristan Richard, Djebbar Atmani
Identification Of Bioactive Compounds From Fraxinus Angustifolia Extracts With Anti-Nadh Oxidase Activity Of Bovine Milk Xanthine Oxidoreductase, Nadjia Ahmane, Dina Atmani-Kilani, Nassima Chaher, Karima Ayouni, Meriem Rahmani-Berboucha, Gregory Da Costa, Nadjet Debbache-Benaida, Tristan Richard, Djebbar Atmani
Turkish Journal of Biology
Fraxinus angustifolia leaves and bark are used in traditional medicine against various inflammatory-related pathologies incumbent to reactive oxygen species (ROS) generation by the NADH oxidase activity of enzymes such as xanthine oxidoreductase (XOR). This study was designed to investigate the in vitro and in vivo inhibitory activities of this enzyme by Fraxinus angustifolia extracts. The leaf organic phase of ethyl acetate (LFA) and its bark aqueous counterpart (BFA) showed the strongest anti-NADH oxidase activity in vitro (IC50 = 38.51 and 42.04 μg mL-1, respectively). They consequently suppressed superoxide generation both enzymatically (53% and 19%, respectively) and nonenzymatically (34% and 19%, …
Functional Characteristics Of Lactobacilli From Traditional Bulgarian Fermented Milk Products, Veronica Nemska, Petya Logar, Tanya Rasheva, Zdravka Sholeva, Nelly Georgieva, Svetla Danova
Functional Characteristics Of Lactobacilli From Traditional Bulgarian Fermented Milk Products, Veronica Nemska, Petya Logar, Tanya Rasheva, Zdravka Sholeva, Nelly Georgieva, Svetla Danova
Turkish Journal of Biology
After oral administration, probiotic lactobacilli meet a number of protection systems in the human body, such as exposure to gastric, pancreatic, and small intestinal juices. Overcoming these detrimental barriers allows living bacteria to adhere to the intestinal epithelium and permanently colonize the gastrointestinal tract (GIT), providing health benefits to the host. Based on this, the transit tolerance of 25 candidate probiotic lactobacilli from katak, yoghurt, and white-brined and yellow cheese to simulated bile and small intestinal juices of variable pH was investigated. To establish their resistance, in vitro model systems based on modified MRS media and a longer duration of …
Key Actors In Cancer Therapy: Epigenetic Modifiers, Remzi̇ Okan Akar, Seli̇n Selvi̇, Engi̇n Ulukaya, Nazlihan Aztopal
Key Actors In Cancer Therapy: Epigenetic Modifiers, Remzi̇ Okan Akar, Seli̇n Selvi̇, Engi̇n Ulukaya, Nazlihan Aztopal
Turkish Journal of Biology
Epigenetic reprogramming plays a crucial role in the tumorigenicity and maintenance of tumor-specific gene expression that especially occurs through DNA methylation and/or histone modifications. It has well-defined mechanisms. It is known that alterations in the DNA methylation pattern and/or the loss of specific histone acetylation/methylation markers are related to several hallmarks of cancer, such as drug resistance, stemness, epithelial-mesenchymal transition, and metastasis. It has also recently been highlighted that epigenetic alterations are critical for the regulation of the stemlike properties of cancer cells (tumor-initiating cells; cancer stem cells). Cancer stem cells are thought to be responsible for the recurrence of …
Characterization Of Mesenchymal Stem Cells In Mucolipidosis Type Ii (I-Cell Disease), Sevi̇l Köse, Fati̇ma Aerts Kaya, Bülent Bariş Kuşkonmaz, Fahri̇ye Duygu Çeti̇nkaya
Characterization Of Mesenchymal Stem Cells In Mucolipidosis Type Ii (I-Cell Disease), Sevi̇l Köse, Fati̇ma Aerts Kaya, Bülent Bariş Kuşkonmaz, Fahri̇ye Duygu Çeti̇nkaya
Turkish Journal of Biology
Mucolipidosis type II (ML-II, I-cell disease) is a fatal inherited lysosomal storage disease caused by a deficiency of the enzyme N-acetylglucosamine-1-phosphotransferase. A characteristic skeletal phenotype is one of the many clinical manifestations of ML-II. Since the mechanisms underlying these skeletal defects in ML-II are not completely understood, we hypothesized that a defect in osteogenic differentiation of ML-II bone marrow mesenchymal stem cells (BM-MSCs) might be responsible for this skeletal phenotype. Here, we assessed and characterized the cellular phenotype of BM-MSCs from a ML-II patient before (BBMT) and after BM transplantation (ABMT), and we compared the results with BM-MSCs from a …
Squalene Attenuates The Oxidative Stress And Activates Akt/Mtor Pathway Against Cisplatin-Induced Kidney Damage In Mice, Ayşe Arzu Şakul, Mehmet Ozansoy, Bi̇rsen Eli̇bol, Şule Ayla, Mehmet Yalçin Günal, Yasemi̇n Yozgat, Selma Hüveyda Başağa, Kazi̇m Şahi̇n, Rümeyza Kazancioğlu, Ülkan Kiliç
Squalene Attenuates The Oxidative Stress And Activates Akt/Mtor Pathway Against Cisplatin-Induced Kidney Damage In Mice, Ayşe Arzu Şakul, Mehmet Ozansoy, Bi̇rsen Eli̇bol, Şule Ayla, Mehmet Yalçin Günal, Yasemi̇n Yozgat, Selma Hüveyda Başağa, Kazi̇m Şahi̇n, Rümeyza Kazancioğlu, Ülkan Kiliç
Turkish Journal of Biology
The clinical use of cisplatin, which is a first-line anticancer agent, is highly restricted due to its adverse effects on kidneys that lead to nephrotoxicity. Therefore, some potential reno-protective substances have been used in combination with cisplatin to cope with nephrotoxicity. Due to its high antitumor activity and oxygen-carrying capacity, we investigated the molecular effects of squalene against cisplatin-induced oxidative stress and kidney damage in mice. Single dose of cisplatin (7 mg/kg) was given to male Balb/c mice. Squalene (100 mg/kg/day) was administered orogastrically to mice for 10 days. Following sacrification, molecular alterations were investigated as analysis of the levels …
Examining The Involvement Of Slx5 In The Apoptotic Response To Chronic Activation Of The Spindle Assembly Checkpoint, Pinar Buket Atalay, Eli̇f Ergi̇n Çavuşoğlu, Öykü Aşci, Duygu Aygüneş
Examining The Involvement Of Slx5 In The Apoptotic Response To Chronic Activation Of The Spindle Assembly Checkpoint, Pinar Buket Atalay, Eli̇f Ergi̇n Çavuşoğlu, Öykü Aşci, Duygu Aygüneş
Turkish Journal of Biology
Microtubule-targeting agents represent one of the most successful groups of anticancer drugs used in cancer therapy today. These drugs induce a prolonged mitotic arrest through chronic spindle assembly checkpoint (SAC) activation. Apoptosis, an outcome of the prolonged mitotic arrest, is the main mechanism by which these anticancer drugs kill cancer cells. However, not much is known about the mechanism that directs chronic SAC activation to apoptosis among other possible outcomes. The aim of this study is to investigate whether Slx5, a sumo-targeted ubiquitin E3 ligase, is involved in directing chronic SAC activation to apoptosis. We show that chronic SAC activation …
Precipitation And Characterization Of Caco3 Of Bacillus Amyloliquefaciens U17 Strain Producing Urease And Carbonic Anhydrase, Merve Tepe, Şevki̇ Arslan, Tamer Koralay, Nazi̇me Mercan
Precipitation And Characterization Of Caco3 Of Bacillus Amyloliquefaciens U17 Strain Producing Urease And Carbonic Anhydrase, Merve Tepe, Şevki̇ Arslan, Tamer Koralay, Nazi̇me Mercan
Turkish Journal of Biology
In the present study, the properties of calcium carbonate mineralization and urease and carbonic anhydrase activities of Bacillus amyloliquefaciens U17 isolated from calcareous soil of Denizli (Turkey) were analyzed. CaCO3 was produced in all growth phases. Strain U17 showed 0.615 ± 0.092 μmol/min/mg urease enzyme activity in calcium mineralization medium and 1.315 ± 0.021 μmol/min/mg urease enzyme activity in Luria-Bertani medium supplemented with urea, whereas it showed 36.03 ± 5.48 nmol/min/ mg carbonic anhydrase enzyme activity in CaCO3 precipitation medium and 28.82 ± 3.31 nmol/min/mg carbonic anhydrase enzyme activity in Luria-Bertani medium supplemented with urea. The urease B protein expression …
Effects Of B2o3 (Boron Trioxide) On Colon Cancer Cells: Our First-Step Experience And In Vitro Results, Özgür Albuz, Di̇lek Dülger, Beste Çağdaş Tunali, Feray Aydin, Seli̇m Yalçin, Mustafa Türk
Effects Of B2o3 (Boron Trioxide) On Colon Cancer Cells: Our First-Step Experience And In Vitro Results, Özgür Albuz, Di̇lek Dülger, Beste Çağdaş Tunali, Feray Aydin, Seli̇m Yalçin, Mustafa Türk
Turkish Journal of Biology
Boron oxide (B2O3) is derived from dehydration of boric acid and is a colorless, semitransparent, crystalline compound that is moderately soluble in water. On the other hand, boron oxide is chemically hygroscopic. This gives the molecule the ability to soak up water and adhere to tissues. Boron oxide can be used locally after tumor debulking in inoperable tumors and especially when the tumor-free margin distance cannot be provided. For all these reasons we aimed to evaluate the in vitro test results of B2O3 in terms of cytotoxicity, genotoxicity, apoptosis, and necrotic effects on L929 fibroblast cells and DLD-1 colorectal adenocarcinoma …
Keratin 14 Is A Novel Interaction Partner Of Keratinocyte Differentiation Regulator:Receptor-Interacting Protein Kinase 4, Ceren Sümer, Asi̇ye Büşra Boz Er, Tuba Di̇nçer
Keratin 14 Is A Novel Interaction Partner Of Keratinocyte Differentiation Regulator:Receptor-Interacting Protein Kinase 4, Ceren Sümer, Asi̇ye Büşra Boz Er, Tuba Di̇nçer
Turkish Journal of Biology
The epidermis, the outer layer of the skin, is formed by stratified keratinocyte layers. The self-renewal of the epidermis is provided by sustained proliferation and differentiation of the keratinocyte stem cells localized to the basal layer of the epidermis. Receptorinteracting protein kinase 4 (RIPK4) is an important regulator of keratinocyte differentiation, mutations of which are associated with congenital ectodermal malformations. In an attempt to identify the molecular basis of RIPK4's function, we applied yeast two-hybrid screen (Y2H) and found basal layer-specific keratin filament component keratin 14 (KRT14) as a novel RIPK4-interacting partner. During keratinocyte differentiation, layer-specific keratin composition is tightly …
A Magnetic Affinity Approach To Identify Plant Gaba-Binding Proteins, Jie Zou, Jingzhe Guo, Shisheng Li
A Magnetic Affinity Approach To Identify Plant Gaba-Binding Proteins, Jie Zou, Jingzhe Guo, Shisheng Li
Turkish Journal of Biology
In plants, GABA plays a critical role in sexual plant reproduction; however, GABA receptors and the associated detailed signaling mechanisms remain to be elucidated. Our experiments show that the proposed technique is reliable and convenient for probing GABA-binding proteins and could be applicable in similar projects by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl). New probes produced by covalently immobilizing the free carboxylic group of GABA on magnetic beads (SiMAG-Carboxyl) can obtain useful information on GABA receptors in plants.
Fibrous Bone Tissue Engineering Scaffolds Prepared By Wet Spinning Of Plga, Nergi̇s Abay Akar, Görke Gürel Peközer, Gamze Köse
Fibrous Bone Tissue Engineering Scaffolds Prepared By Wet Spinning Of Plga, Nergi̇s Abay Akar, Görke Gürel Peközer, Gamze Köse
Turkish Journal of Biology
Having a self-healing capacity, bone is very well known to regenerate itself without leaving a scar. However, critical size defects due to trauma, tumor, disease, or infection involve bone graft surgeries in which complication rate is relatively at high levels. Bone tissue engineering appears as an alternative for grafting. Fibrous scaffolds are useful in tissue engineering applications since they have a high surface-to-volume ratio, and adjustable, highly interconnected porosity to enhance cell adhesion, survival, migration, and proliferation. They can be produced in a wide variety of fiber sizes and organizations. Wet spinning is a convenient way to produce fibrous scaffolds …
A Novel 1,4-Naphthoquinone-Derived Compound Induces Apoptotic Cell Deathin Breast Cancer Cells, Di̇dem Karakaş Zeybek, Remzi̇ Okan Akar, Zeli̇ha Gökmen, Nahi̇de Gülşah Deni̇z, Engi̇n Ulukaya
A Novel 1,4-Naphthoquinone-Derived Compound Induces Apoptotic Cell Deathin Breast Cancer Cells, Di̇dem Karakaş Zeybek, Remzi̇ Okan Akar, Zeli̇ha Gökmen, Nahi̇de Gülşah Deni̇z, Engi̇n Ulukaya
Turkish Journal of Biology
Breast cancer is the most-diagnosed cancer type among women. The triple-negative subtype is an especially aggressive type of breast cancer. Although chemotherapy is almost the only option for the treatment of triple-negative breast cancer (TNBC), currently used chemotherapeutics are not effective enough, considering the poor survival rate of patients. Therefore, novel compounds need to be developed to improve survival rates. It has been known that quinonic compounds, which are found in nature, have antibacterial, antifungal, and antitumorigenic properties. Naphthoquinones are members of the quinone family and are widely used in research due to their promising properties. In this study, we …
Characterizing Microsatellite Polymorphisms Using Assembly-Based Andmapping-Based Tools, Gülfem Demi̇r, Can Alkan
Characterizing Microsatellite Polymorphisms Using Assembly-Based Andmapping-Based Tools, Gülfem Demi̇r, Can Alkan
Turkish Journal of Biology
Microsatellite polymorphism has always been a challenge for genome assembly and sequence alignment due to sequencing errors, short read lengths, and high incidence of polymerase slippage in microsatellite regions. Despite the information they carry being very valuable, microsatellite variations have not gained enough attention to be a routine step in genome sequence analysis pipelines. After the completion of the 1000 Genomes Project, which aimed to establish the most detailed genetic variation catalog for humans, the consortium released only two microsatellite prediction sets generated by two tools. Many other large research efforts have failed to shed light on microsatellite variations. We …
Microrna Prediction Based On 3d Graphical Representation Of Rnasecondary Structures, Müşerref Duygu Saçar Demi̇rci̇
Microrna Prediction Based On 3d Graphical Representation Of Rnasecondary Structures, Müşerref Duygu Saçar Demi̇rci̇
Turkish Journal of Biology
MicroRNAs (miRNAs) are posttranscriptional regulators of gene expression. While a miRNA can target hundreds of messenger RNA (mRNAs), an mRNA can be targeted by different miRNAs, not to mention that a single miRNA might have various binding sites in an mRNA sequence. Therefore, it is quite involved to investigate miRNAs experimentally. Thus, machine learning (ML) is frequently used to overcome such challenges. The key parts of a ML analysis largely depend on the quality of input data and the capacity of the features describing the data. Previously, more than 1000 features were suggested for miRNAs. Here, it is shown that …
Knockdown Of Set Domain, Bifurcated 1 Suppresses Head And Neck Cancer Cell Viability And Wound-Healing Ability In Vitro, Si̇bel Özdaş
Knockdown Of Set Domain, Bifurcated 1 Suppresses Head And Neck Cancer Cell Viability And Wound-Healing Ability In Vitro, Si̇bel Özdaş
Turkish Journal of Biology
Head and neck cancer (HNC) is the sixth most common cancer worldwide and therefore presents a global public health problem. There are no standard algorithms for the diagnosis and follow-up of the disease, and no effective current treatment approaches exist. Therefore, the discovery of new biomolecules and the design of new strategies to aid in early diagnosis is necessary, along with establishing prognostic factors of HNC. In several cancer studies, the upregulation of SET Domain, Bifurcated 1 (SETDB1) has been reported to be tumor-inducing and to indicate a cancer-invasive prognosis, leading to the modulation of genes associated with different signaling …
Prenatal Exposure Of Diclofenac Sodium Alters The Behavioral Development Of Young Wistar Rats, Bi̇rsen Eli̇bol, Begüm Oğur, Havva Doğru
Prenatal Exposure Of Diclofenac Sodium Alters The Behavioral Development Of Young Wistar Rats, Bi̇rsen Eli̇bol, Begüm Oğur, Havva Doğru
Turkish Journal of Biology
Diclofenac sodium (DS), a potent inhibitor of cyclooxygenase, reduces the release of arachidonic acid and formation of prostaglandins. Being a nonsteroid drug that shows antiinflammatory action, the possible side effects of fetal DS administration gain importance in public and medical applications. Herein, the effects of DS administration (1 mg/kg) during gestational days 5-20 were investigated on the performance of Wistar rat pups in a variety of behavioral tasks. Four-week-old pups were subjected to sensory motor tests, a plus maze, an open field, the Morris water maze, and a radial arm maze. Fetal DS disrupted some sensory motor performances, such as …
A New Helicoverpa Armigera Nucleopolyhedrovirus Isolate From Heliothis Peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) In Turkey, Gözde Büşra Eroğlu, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
A New Helicoverpa Armigera Nucleopolyhedrovirus Isolate From Heliothis Peltigera (Denis & Schiffermuller) (Lepidoptera: Noctuidae) In Turkey, Gözde Büşra Eroğlu, Remzi̇ye Nalçacioğlu, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
This study reports a new Helicoverpa armigera nucleopolyhedrovirus (NPV) isolated from Heliothis peltigera (Denis & Schiffermuller), collected in the vicinity of Adana, Turkey. Infection was confirmed by tissue polymerase chain reaction and sequence analysis. Results showed that dead H. peltigera larvae contain Helicoverpa armigera nucleopolyhedrovirus. Thus, the isolate was named as HearNPV-TR. Microscopy studies indicated that occlusion bodies were 0.73 to 1.66 μm in diameter. The nucleocapsids are approximately 184 × 41 nm in size. The genome of HearNPV-TR was digested with KpnI and XhoI enzymes and calculated as 130.5 kb. Phylogenetic analysis showed that HearNPV-TR has close relation with …
Cytotoxic And Genotoxic Action Of Tagetes Patula Flower Methanol Extract And Patuletin Using The Allium Test, Mudassar Azhar, Ahsana Dar Farooq, Sayedul Haque, Samina Bano, Lubna Zaheer, Shaheen Faizi
Cytotoxic And Genotoxic Action Of Tagetes Patula Flower Methanol Extract And Patuletin Using The Allium Test, Mudassar Azhar, Ahsana Dar Farooq, Sayedul Haque, Samina Bano, Lubna Zaheer, Shaheen Faizi
Turkish Journal of Biology
Tagetes patula is used to treat cancer patients in alternative healthcare systems. However, its cytotoxic and genotoxic effects have not been reported. Therefore, the methanol extract of T. patula flower, the ethyl acetate fraction, and the pure compound patuletin were evaluated using the Allium test. The methanol extract and fraction contained ~3% and ~36% patuletin, respectively, with ~98% purity. The methanol extract caused inhibition of Allium root growth displaying an IC50 value of ~500 μg/mL, while the fraction and patuletin were more potent by ~2 and ~5 times, respectively. The Allium root tips demonstrated a decline in prophase, metaphase, anaphase, …
The Nlrp3 Inflammasome: A New Player In Neurological Diseases, Eli̇f Eren, Nesri̇n Özören
The Nlrp3 Inflammasome: A New Player In Neurological Diseases, Eli̇f Eren, Nesri̇n Özören
Turkish Journal of Biology
Inflammasomes are supramolecular protein complexes implicated in the detection of pathogens or danger-associated molecules and are responsible for mounting the first line of innate immune response to counteract these signals and restore tissue homeostasis. Among different inflammasomes identified so far, NLRP3 is of main interest since mutations in Nlrp3 gene are associated with autoinflammatory diseases such as Muckle-Wells syndrome, neonatal onset multisystem inflammatory disease, and familial cold urticaria/autoinflammatory syndrome. On the other hand, whereas other inflammasomes are mainly detectors of specific molecular motifs, NLRP3 is acting as a general sensor of cellular perturbations including potassium efflux, lysosomal damage, and ROS …
In Vitro Study On Chondrogenic Differentiation Of Human Adipose-Derived Stem Cells On Treated Bovine Pericardium, My Thi Ngoc Nguyen, Vu Nguyen Doan, Ha Le Bao Tran
In Vitro Study On Chondrogenic Differentiation Of Human Adipose-Derived Stem Cells On Treated Bovine Pericardium, My Thi Ngoc Nguyen, Vu Nguyen Doan, Ha Le Bao Tran
Turkish Journal of Biology
Bovine pericardium has been proposed as an available material for tissue engineering and bioprosthetic reconstruction. In this study, bovine pericardium was fabricated into a scaffold for culturing and chondrogenic differentiation of human adipose-derived stem cells (hADSCs). Bovine pericardium was treated in 10 mM Tris-HCl and 0.15% SDS, followed by crosslinking in 0.1% glutaraldehyde. Treated bovine pericardium (tBP) was characterized as a slight yellowish thin membrane with enhanced tensile strength and strain property. The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP as a cell-friendly scaffold for hADSCs due to low cytotoxicity …
In Silico Approach To The Analysis Of Snps In The Human Apaf1 Gene, Tuğba Kaman, Ömer Faruk Karasakal, Ebru Özkan Oktay, Korkut Ulucan, Muhsi̇n Konuk
In Silico Approach To The Analysis Of Snps In The Human Apaf1 Gene, Tuğba Kaman, Ömer Faruk Karasakal, Ebru Özkan Oktay, Korkut Ulucan, Muhsi̇n Konuk
Turkish Journal of Biology
The apoptotic protease activating factor 1 (APAF1) gene encodes a cytoplasmic protein that initiates apoptosis and is a crucial factor in the mitochondria-dependent death pathway. APAF1 is implicated in many pathways such as apoptosis, neurodegenerative diseases, and cancer. The purpose of this study was to predict deleterious/damaging SNPs in the APAF1 gene via in silico analysis. To this end, APAF1 missense SNPs were obtained from the NCBI dbSNP database. In silico analysis of the missense SNPs was carried out by using publicly available online software tools. The stabilization and three-dimensional modeling of mutant proteins were also determined by using the …
Crosstalk Between Autophagy And Apoptosis Induced By Camphor Inschizosaccharomyces Pombe, Hizlan Hincal Ağuş, Sedanur Yilmaz, Cansin Ogeday Şengöz
Crosstalk Between Autophagy And Apoptosis Induced By Camphor Inschizosaccharomyces Pombe, Hizlan Hincal Ağuş, Sedanur Yilmaz, Cansin Ogeday Şengöz
Turkish Journal of Biology
Camphor is widely used in pharmacy, the food industry, and cosmetics. In this study, we evaluate inhibitory and cytotoxic effects of camphor in the fission yeast (Schizosaccharomyces pombe), which presents a unicellular model in mechanistic toxicology and cell biology. Low-dose camphor exposure (0.4 mg/mL) activated autophagy, which was shown by GFP-Atg8 dots and transcriptional upregulation of Atg6 (Beclin-1 ortholog). Autophagy was also confirmed by using autophagy-deficient cells, which showed reduction in GFP-Atg8 dot formation. However, high-dose camphor exposure (0.8 mg/mL) caused dramatic cell death ratios, demonstrated by spot and colony-forming assays, even in autophagy-deficient cells. To unravel the underlying mechanism, …
Identification Of Watermelon Heat Shock Protein Members And Tissue-Specific Gene Expression Analysis Under Combined Drought And Heat Stresses, Yasemi̇n Çeli̇k Altunoğlu, Merve Keleş, Tevfi̇k Hasan Can, Mehmet Cengi̇z Baloğlu
Identification Of Watermelon Heat Shock Protein Members And Tissue-Specific Gene Expression Analysis Under Combined Drought And Heat Stresses, Yasemi̇n Çeli̇k Altunoğlu, Merve Keleş, Tevfi̇k Hasan Can, Mehmet Cengi̇z Baloğlu
Turkish Journal of Biology
Heat shock protein (Hsp) gene family members in the watermelon genome were identified and characterized by bioinformatics analysis. In addition, expression profiles of genes under combined drought and heat stress conditions were experimentally analyzed. In the watermelon genome, 39 genes belonging to the sHsp family, 101 genes belonging to the Hsp40 family, 23 genes belonging to the Hsp60 family, 12 genes belonging to the Hsp70 family, 6 genes belonging to the Hsp90 family, and 102 genes belonging to the Hsp100 family were found. It was also observed that the proteins in the same cluster in the phylogenetic trees had similar …
Comparison Of A Ceramidase Inhibitor (Ceranib-2) With C2 Ceramide And Cisplatin On Cytotoxicity And Apoptosis Of Glioma Cells, Gökhan Kuş, Mete Özkurt, Ayşe Pinar Öztopcu Vatan, Ni̇lüfer Erkasap, Ruhi̇ Uyar, Selda Kabadere
Comparison Of A Ceramidase Inhibitor (Ceranib-2) With C2 Ceramide And Cisplatin On Cytotoxicity And Apoptosis Of Glioma Cells, Gökhan Kuş, Mete Özkurt, Ayşe Pinar Öztopcu Vatan, Ni̇lüfer Erkasap, Ruhi̇ Uyar, Selda Kabadere
Turkish Journal of Biology
Inhibiting ceramidase activity in cancer cells has been identified as a promising target for cancer therapy in recent studies. Thus, we examined the possible role of ceranib-2, a novel ceramidase inhibitor, on growth and apoptotic mechanisms of the human normal glia cell line (HNA), human glioma cell lines (T-98G and U-87MG), and a rat glioma cell line (C6). We also compared the results with the effects of C2 ceramide and cisplatin. We determined the in vitro survival rate with MTT assay, apoptosis with flow cytometry, gene expressions with qRT-PCR, and statistical significance by one-way analysis of variance together with Tukey's …
Biogenic Production Of Silver Nanoparticles By Enterobacter Cloacae Ism26, Khaled Elbaghdady, Einas El-Shatoury, Omnia Abdallah, Mostafa Khalil
Biogenic Production Of Silver Nanoparticles By Enterobacter Cloacae Ism26, Khaled Elbaghdady, Einas El-Shatoury, Omnia Abdallah, Mostafa Khalil
Turkish Journal of Biology
A bacterial isolate capable of tolerating 30 mM silver nitrate (AgNO3) was recovered from soil contaminated with industrial waste. The isolate was identified by 16S rRNA as
Comparative Study Of Mesenchymal Stem Cells From Rat Bone Marrow And Adipose Tissue, Qing He, Zhaoyang Ye, Yan Zhou, Wen-Song Tan
Comparative Study Of Mesenchymal Stem Cells From Rat Bone Marrow And Adipose Tissue, Qing He, Zhaoyang Ye, Yan Zhou, Wen-Song Tan
Turkish Journal of Biology
Several therapeutic products based on mesenchymal stem cells (MSCs) have been translated into clinical applications. MSCs should undergo in vitro culture before a sufficient quantity can be achieved. Hence, both expansion kinetics and the biological characteristics of derived cells from primary culture are pertinent to their applications. In the present study, MSCs were isolated from rat bone marrow and adipose tissue (designated as bMSCs and aMSCs, respectively) and cells were comparatively analyzed regarding cell morphology, proliferation, colony formation, differentiation potential, and immunophenotype following the long-term subculture. No apparent differences could be noticed concerning the morphology between bMSCs and aMSCs. The …
Cloning And Soluble Expression Of Mature ${\Alpha}$-Luffin From Luffa Cylindrica In E. Coli Using Sumo Fusion Protein, Shaghayegh Namvar, Farzaneh Barkhordari, Mozhgan Raigani, Hoda Jahandar, Leila Nematollahi, Fatemeh Davami
Cloning And Soluble Expression Of Mature ${\Alpha}$-Luffin From Luffa Cylindrica In E. Coli Using Sumo Fusion Protein, Shaghayegh Namvar, Farzaneh Barkhordari, Mozhgan Raigani, Hoda Jahandar, Leila Nematollahi, Fatemeh Davami
Turkish Journal of Biology
${\alpha}$-Luffin, found in Luffa cylindrica seeds, is a type I ribosome inactivating proteins. Cytotoxic effects make it an appropriate candidate for the construction of immunotoxins and conjugates. Because of limited natural resources, recombinant technology is the best approach to achieve large-scale production of plant-based proteins. In the present study, ${\alpha}$-luffin protein was expressed in E. coli and the effects of different temperature conditions, SUMO fusion tag, and cultivation strategies on total expression and solubility were investigated. Protein expression was evaluated at different intervals (0, 4, 6, 8, 24 h) postinduction. Our results showed that EnBase had higher efficiency than LB, …
Combined Extract Of Moringa Oleifera And Centella Asiatica Modulates Oxidative Stress And Senescence In Hydrogen Peroxide-Induced Human Dermal Fibroblasts, Elly Ezlinda Abdul Hisam, Mohd Salleh Rofiee, Aina Mardhia Khalid, Ahmad Firdaus Jalaluddin, Mohd Izwan Muhammad Yusof, Muhd Hanis Idris, Salfarina Ramli, Richard Johari James, Wee Jack Yoeng, Teh Lay Kek, Mohd Zaki Salleh
Combined Extract Of Moringa Oleifera And Centella Asiatica Modulates Oxidative Stress And Senescence In Hydrogen Peroxide-Induced Human Dermal Fibroblasts, Elly Ezlinda Abdul Hisam, Mohd Salleh Rofiee, Aina Mardhia Khalid, Ahmad Firdaus Jalaluddin, Mohd Izwan Muhammad Yusof, Muhd Hanis Idris, Salfarina Ramli, Richard Johari James, Wee Jack Yoeng, Teh Lay Kek, Mohd Zaki Salleh
Turkish Journal of Biology
Moringa oleifera Lam. and Centella asiatica (L.) Urb. leaves have been previously reported to exhibit antioxidant activity. The objective of the present study is to determine the in vitro antioxidant activity of the combined extracts of M. oleifera and C. asiatica (TGT-PRIMAAGE) and its effect on hydrogen peroxide (H$_{2}$O$_{2}$)-induced oxidative stress in human dermal fibroblasts. TGT-PRIMAAGE acted on the mechanism of hydrogen transfer as it showed scavenging activity in the DPPH assay. This is due to the presence of phenolics and flavonoids in TGT-PRIMAAGE. TGT-PRIMAAGE effectively reduced cellular generation of reactive oxygen species induced by H$_{2}$O$_{2}$. The activities of superoxide …
Crm1 Knockdown By Specific Small Interfering Rna Reduces Cell Proliferation And Induces Apoptosis In Head And Neck Cancer Cell Lines, Si̇bel Özdaş, Tali̇h Özdaş
Crm1 Knockdown By Specific Small Interfering Rna Reduces Cell Proliferation And Induces Apoptosis In Head And Neck Cancer Cell Lines, Si̇bel Özdaş, Tali̇h Özdaş
Turkish Journal of Biology
Head and neck squamous cell carcinoma (HNSCC) is the most common and most aggressive type of head and neck cancer. Current approaches for the treatment of HNSCC are not sufficient to increase the patient survival or to reduce the high recurrence rate. Consequently, there is a need to explore the molecular characteristics of this cancer in order to discover potential therapeutic target molecules. The overexpression of chromosome region maintenance 1 (Crm1), responsible for the transport of different classes of macromolecules from the nuclear membrane to the cytoplasm, in various cancer cells has made it an attractive target molecule in cancer …