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Articles 301 - 330 of 1681
Full-Text Articles in Biology
The Methanolic Extract Of Garcinia Atroviridis (Mega) Reduces Body Weight And Food Intake, And Improves Lipid Profiles By Altering The Lipid Metabolism: A Rat Model, Wai Feng Lim, Suriati Mohd Nasir, Lay Kek Teh, Richard Johari James, Mohd Hafidz Mohd Izhar, Mohd Zaki Salleh
The Methanolic Extract Of Garcinia Atroviridis (Mega) Reduces Body Weight And Food Intake, And Improves Lipid Profiles By Altering The Lipid Metabolism: A Rat Model, Wai Feng Lim, Suriati Mohd Nasir, Lay Kek Teh, Richard Johari James, Mohd Hafidz Mohd Izhar, Mohd Zaki Salleh
Turkish Journal of Biology
Garcinia species are widely used for their slimming effects via increased fat burning and suppression of satiety. However, scientific evidence for the biological effects of Garcinia atroviridis (GA) is lacking. We investigated the phytochemical composition, safety profiles, and antioxidant and antiobesity effects of methanolic extracts of Garcinia atroviridis (MeGa) in obese female rats. Repeated dose toxicity studies were conducted according to the OECD guidelines. Upon sacrifice, haematological, biochemical, lipid profile, and serum-based metabolomics analyses were performed to evaluate metabolic expression changes and their related pathways. MeGa contains several phytochemical groups and GA fruit acids. MeGa was found to be nontoxic …
An Insight Into The Epitope-Based Peptide Vaccine Design Strategy And Studies Against Covid-19, Murat Topuzoğullari, Tayfun Acar, Peli̇n Peli̇t Arayici, Burcu Uçar, Erennur Uğurel, Emrah Şefi̇k Abamor, Tüli̇n Arasoğlu, Di̇lek Balik, Serap Derman
An Insight Into The Epitope-Based Peptide Vaccine Design Strategy And Studies Against Covid-19, Murat Topuzoğullari, Tayfun Acar, Peli̇n Peli̇t Arayici, Burcu Uçar, Erennur Uğurel, Emrah Şefi̇k Abamor, Tüli̇n Arasoğlu, Di̇lek Balik, Serap Derman
Turkish Journal of Biology
SARS-CoV-2 is a new member of the coronavirus family and caused the pandemic of coronavirus disease 2019 (COVID-19) in 2020. It is crucial to design and produce an effective vaccine for the prevention of rapid transmission and possible deaths wcaused by the disease. Although intensive work and research are being carried out all over the world to develop a vaccine, an effective and approved formulation that can prevent the infection and limit the outbreak has not been announced yet. Among all types of vaccines, epitope-based peptide vaccines outshine with their low-cost production, easy modification in the structure, and safety. In …
Astragalus Membranaceus And Punica Granatum Alleviate Infertility And Kidney Dysfunction Induced By Aging In Male Rats, Ameera Alshinnawy, Wael Elsayed, Alshaimaa Taha, Ahmed Sayed, Ahmed Salem
Astragalus Membranaceus And Punica Granatum Alleviate Infertility And Kidney Dysfunction Induced By Aging In Male Rats, Ameera Alshinnawy, Wael Elsayed, Alshaimaa Taha, Ahmed Sayed, Ahmed Salem
Turkish Journal of Biology
By aging, male fertility and kidney function decline. Therefore, the investigation of health span-extending agents becomes more urgent to overcome aging-induced infertility and kidney dysfunction. The current research was undertaken to investigate the antiaging efficacy of Astragalus membranaceus telomerase activator-65 (Ta-65) and pomegranate supplements. Forty male Wistar rats were divided into young rats, aged rats, aged rats treated with Ta-65 (500mg/kg/day), and aged rats treated with pomegranate (250mg/kg/day). Testosterone, FSH, LH, and kidney functions were measured in serum. Sperm analysis as well as testicular histological examination was performed. Aging caused an imbalance in male sex hormones resulting in sperm abnormality …
Two Boron-Containing Compounds Affect The Cellular Viability Of Sh-Sy5y Cells In An In Vitro Amyloid-Beta Toxicity Model, Mehmet Ozansoy, Mehmet Özgen Altintaş, Muzaffer Beyza Ozansoy, Necmeddi̇n Günay, Ertuğrul Kiliç, Ülkan Kiliç
Two Boron-Containing Compounds Affect The Cellular Viability Of Sh-Sy5y Cells In An In Vitro Amyloid-Beta Toxicity Model, Mehmet Ozansoy, Mehmet Özgen Altintaş, Muzaffer Beyza Ozansoy, Necmeddi̇n Günay, Ertuğrul Kiliç, Ülkan Kiliç
Turkish Journal of Biology
Boron is a naturally occurring trace element found in organic and inorganic complexes. Boron-containing compounds are required for living organisms for diverse metabolic functions, including nitrogen fixation in microorganisms, cell wall stability in plants, and bone and carbohydrate metabolism in animals. The number of studies about the effect of boron in biological model systems is very limited; so far, there has been no study on the correlation between boron and amyloid-beta toxicity. Here, we investigated the possible effects of 2 boron-containing compounds-sodium borate decahydrate and boric acid-against amyloid-beta toxicity. In our in vitro amyloid-beta toxicity model, we showed that these …
Genetic Alterations In B Cell Lymphoma Subtypes As Potential Biomarkers For Noninvasive Diagnosis, Prognosis, Therapy, And Disease Monitoring, Esra Esmeray, Can Küçük
Genetic Alterations In B Cell Lymphoma Subtypes As Potential Biomarkers For Noninvasive Diagnosis, Prognosis, Therapy, And Disease Monitoring, Esra Esmeray, Can Küçük
Turkish Journal of Biology
Neoplastic transformation of germinal center B (GCB) cells may give rise to a variety of different B cell lymphoma subtypes, most of which show substantial heterogeneity in terms of genetic alterations and clinical features. The mutations observed in cancer-related genes in GCB cells are related to abnormalities in the immunogenetic mechanisms associated with germinal center reaction. Recent studies have rapidly identified genomic alterations in B cell lymphomas that may be useful for better subclassification, noninvasive diagnosis, and prediction of response to therapy. The WHO recognizes different lymphoma subsets classified within 2 major categories of B cell lymphoma: Hodgkin's lymphoma (HL) …
Doxorubicin Inhibits Mir-140 Expression And Upregulates Pd-L1 Expression In Hct116 Cells, Opposite To Its Effects On Mda-Mb-231 Cells, Noel Mopouguini Naba, Nazi̇fe Tolay, Mehmet Batu Erman, Ayça Sayi Yazgan
Doxorubicin Inhibits Mir-140 Expression And Upregulates Pd-L1 Expression In Hct116 Cells, Opposite To Its Effects On Mda-Mb-231 Cells, Noel Mopouguini Naba, Nazi̇fe Tolay, Mehmet Batu Erman, Ayça Sayi Yazgan
Turkish Journal of Biology
One of the most challenging problems in colorectal cancer (CRC) is resistance to chemotherapy drugs such as doxorubicin (DOX). The programmed death ligand-1 (PD-L1) is related to chemoresistance and is overexpressed in several human cancer cell types. Here, we investigated the changes in the expression of PD-L1 in DOX-treated CRC and breast cancer (BRC) cells. Also, to address PD-L1 regulation, we assessed expression levels of miR-140 and miR-34a, two microRNAs that can target the 3' UTR region of the gene encoding PD-L1. HCT116 CRC and MDA-MB-231 BRC cells were treated with various doses of DOX in culture and PD-L1 expression …
The Effect Of R547, A Cyclin-Dependent Kinase Inhibitor, On Hepatocellular Carcinoma Cell Death, Betül Hacioğlu, Gökhan Kuş, Hati̇ce Mehtap Kutlu, Selda Kabadere
The Effect Of R547, A Cyclin-Dependent Kinase Inhibitor, On Hepatocellular Carcinoma Cell Death, Betül Hacioğlu, Gökhan Kuş, Hati̇ce Mehtap Kutlu, Selda Kabadere
Turkish Journal of Biology
Hepatocellular carcinoma (HCC) is the third main cause of cancer-related death. Cyclin-dependent kinases (CDKs) and their cyclin partners regulate the cell cycle. Since inhibition of CDKs gives some guiding ideas for cancer studies, we aimed to determine the possible effects of R547, a cyclin kinase 1-2-4 inhibitor, on proliferation and apoptotic mechanisms of Hep G2 cells (human) and H-4- II-E cells derived from rat HCC. We determined in vitro survival rates with MTT assay, apoptosis with flow cytometry, morphological changes with confocal microscopy, and ultrastructural changes by transmission electron microscopy. Cisplatin was used as a positive control. After 24 h …
Oncogenic And Tumor Suppressor Function Of Meis And Associated Factors, Bi̇rkan Gi̇rgi̇n, Medi̇ne Karadağ Alpaslan, Fati̇h Kocabaş
Oncogenic And Tumor Suppressor Function Of Meis And Associated Factors, Bi̇rkan Gi̇rgi̇n, Medi̇ne Karadağ Alpaslan, Fati̇h Kocabaş
Turkish Journal of Biology
MEIS proteins are historically associated with tumorigenesis, metastasis, and invasion in cancer. MEIS and associated PBXHOX proteins may act as tumor suppressors or oncogenes in different cellular settings. Their expressions tend to be misregulated in various cancers. Bioinformatic analyses have suggested their upregulation in leukemia/lymphoma, thymoma, pancreas, glioma, and glioblastoma, and downregulation in cervical, uterine, rectum, and colon cancers. However, every cancer type includes, at least, a subtype with high MEIS expression. In addition, studies have highlighted that MEIS proteins and associated factors may function as diagnostic or therapeutic biomarkers for various diseases. Herein, MEIS proteins and associated factors in …
Expression Pattern Of Olfactory Receptor Genes In Human Cumulus Cells As An Indicator For Competent Oocyte Selection, Neda Daei-Farshbaf, Reza Aflatoonian, Fatemeh-Sadat Amjadi, Sara Taleahmad, Mahnaz Ashrafi, Mehrdad Bakhtiyari
Expression Pattern Of Olfactory Receptor Genes In Human Cumulus Cells As An Indicator For Competent Oocyte Selection, Neda Daei-Farshbaf, Reza Aflatoonian, Fatemeh-Sadat Amjadi, Sara Taleahmad, Mahnaz Ashrafi, Mehrdad Bakhtiyari
Turkish Journal of Biology
Odorant or olfactory receptors are mainly localized in the olfactory epithelium for the perception of different odors. Interestingly, many ectopic olfactory receptors with low expression levels have recently been found in nonolfactory tissues to involve in local functions. Therefore, we investigated the probable role of the olfactory signaling pathway in the surrounding microenvironment of oocyte. This study included 22 women in intracytoplasmic sperm injection cycle. The expression of olfactory target molecules in cumulus cells surrounding the growing and mature oocytes was evaluated by Western blotting and real-time polymerase chain reaction. Additionally, integrated bioinformatics analyses were carried out and 6 ectopic …
Ptdins(4,5)P2 And Ptdins(3,4,5)P3 Dynamics During Focal Adhesions Assembly And Disassembly In A Cancer Cell Line, Dhurgham Alfahad, Salem Alharethi, Bandar Alharbi, Khatab Mawlood, Philip Dash
Ptdins(4,5)P2 And Ptdins(3,4,5)P3 Dynamics During Focal Adhesions Assembly And Disassembly In A Cancer Cell Line, Dhurgham Alfahad, Salem Alharethi, Bandar Alharbi, Khatab Mawlood, Philip Dash
Turkish Journal of Biology
Focal adhesions (FAs) are large assemblies of proteins that mediate intracellular signals between the cytoskeleton and the extracellular matrix (ECM). The turnover of FA proteins plays a critical regulatory role in cancer cell migration. Plasma membrane lipids locally generated or broken down by different inositide kinases and phosphatase enzymes to activate and recruit proteins to specific regions in the plasma membrane. Presently, little attention has been given to the use of phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and Phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) fluorescent biosensors in order to determine the spatiotemporal organisation of PtdIns(4,5)P2 and PtdIns(3,4,5)P3 within and around or during assembly and disassembly of …
Expression Of Soluble, Active, Fluorescently Tagged Hephaestin In Cos And Cho Cell Lines, Eli̇f Si̇bel Aslan, Kenneth N. White, Basharut A. Syed, Kaila S. Srai, Robert W. Evans
Expression Of Soluble, Active, Fluorescently Tagged Hephaestin In Cos And Cho Cell Lines, Eli̇f Si̇bel Aslan, Kenneth N. White, Basharut A. Syed, Kaila S. Srai, Robert W. Evans
Turkish Journal of Biology
Hephaestin (Hp) is a trans-membrane protein, which plays a critical role in intestinal iron absorption. Hp was originally identified as the gene responsible for the phenotype of sex-linked anaemia in the sla mouse. The mutation in the sla protein causes accumulation of dietary iron in duodenal cells, causing severe microcytic hypochromic anaemia. Although mucosal uptake of dietary iron is normal, export from the duodenum is inhibited. Hp is homologous to ceruloplasmin (Cp), a member of the family of multi copper ferroxidases (MCFs) and possesses ferroxidase activity that facilitates iron release from the duodenum and load onto the serum iron transport …
Sex Effect On The Correlation Of Immunoglobulin G Glycosylation With Rheumatoid Arthritis Disease Activity, Altan Ercan
Sex Effect On The Correlation Of Immunoglobulin G Glycosylation With Rheumatoid Arthritis Disease Activity, Altan Ercan
Turkish Journal of Biology
Rheumatoid arthritis (RA) is a chronic autoimmune disease which affects females more than males with a presence of autoantibodies. Immunoglobulin G (IgG) produced by adaptive arm has 2 functional domains, Fc and Fab. The Fc domain binds Fc gamma receptors and C1q proteins of the innate arm. Therefore, the IgG Fc domain serves as a bridge between the innate and adaptive arms and is regulated by an evolutionarily conserved N-glycosylation with variable structures. These glycans are classified as agalactosylated G0, monogalactosylated G1, and digalactosylated G2, which are further modified by core-fucosylation (F) and bisecting N-acetylglucosamine (B) moieties such as G0F …
The Inhibitory Effect Of Melatonin On Osteoclastogenesis Of Raw 264.7 Cells In Low Concentrations Of Rankl And Mcsf, Hala Jarrar, Damla Çeti̇n Altindal, Menemşe Gümüşdereli̇oğlu
The Inhibitory Effect Of Melatonin On Osteoclastogenesis Of Raw 264.7 Cells In Low Concentrations Of Rankl And Mcsf, Hala Jarrar, Damla Çeti̇n Altindal, Menemşe Gümüşdereli̇oğlu
Turkish Journal of Biology
RAW 264.7 cells are one of the most recommended cell lines for investigating the activity and differentiation of osteoclasts. These cells differentiate into osteoclasts in the presence of two critical components: receptor activator of nuclear factor kappa B ligand (RANKL) and macrophage colony stimulating factor (MCSF). Melatonin (MEL) hormone has recently become one of the small molecules used in the field of bone regeneration and bone disease treatment, as it has the ability to inhibit the differentiation of osteoclasts directly by suppression of the NF-κB signaling pathway. The main aim of the current study is to determine sufficient RANKL/MCSF concentrations …
Genome-Wide Identification Of Chiari Malformation Type I Associated Candidate Genes And Chromosomal Variations, Ti̇muçi̇n Avşar, Şeyma Çaliş, Baran Yilmaz, Gülden Demi̇rci̇ Otluoğlu, Can Holyavki̇n, Türker Kiliç
Genome-Wide Identification Of Chiari Malformation Type I Associated Candidate Genes And Chromosomal Variations, Ti̇muçi̇n Avşar, Şeyma Çaliş, Baran Yilmaz, Gülden Demi̇rci̇ Otluoğlu, Can Holyavki̇n, Türker Kiliç
Turkish Journal of Biology
Chiari malformation type I (CMI) is a brain malformation that is characterized by herniation of the cerebellum into the spinal canal. Chiari malformation type I is highly heterogeneous; therefore, an accurate explanation of the pathogenesis of the disease is often not possible. Although some studies showed the role of genetics in CMI, the involvement of genetic variations in CMI pathogenesis has not been thoroughly elucidated. Therefore, in the current study we aim to reveal CMI-associated genomic variations in familial cases. Four CMI patients and 7 unaffected healthy members of two distinct families were analyzed. A microarray analysis of the affected …
Protein Engineering Approaches For Antibody Fragments: Directed Evolution And Rational Design Approaches, Merve Arslan, Di̇lara Karadağ, Si̇bel Kalyoncu
Protein Engineering Approaches For Antibody Fragments: Directed Evolution And Rational Design Approaches, Merve Arslan, Di̇lara Karadağ, Si̇bel Kalyoncu
Turkish Journal of Biology
The number of therapeutic antibodies in preclinical, clinical, or approved phases has been increasing exponentially, mostly due to their known successes. Development of antibody engineering methods has substantially hastened the development of therapeutic antibodies. A variety of protein engineering techniques can be applied to antibodies to improve their affinity and/or biophysical properties such as solubility and stability. Antibody fragments (where all or some parts of constant regions are eliminated while the essential antigen binding region is preserved) are more suitable for protein engineering techniques because there are many in vitro screening technologies available for antibody fragments but not full-length antibodies. …
Prediction And Expression Analysis Of G Protein-Coupled Receptors In The Laboratory Stick Insect, Carausius Morosus, Burçi̇n Duan Şahbaz, Necla Bi̇rgül İyi̇son
Prediction And Expression Analysis Of G Protein-Coupled Receptors In The Laboratory Stick Insect, Carausius Morosus, Burçi̇n Duan Şahbaz, Necla Bi̇rgül İyi̇son
Turkish Journal of Biology
G protein-coupled receptors (GPCRs) are 7-transmembrane proteins that transduce various extracellular signals into intracellular pathways. They are the major target of neuropeptides, which regulate the development, feeding behavior, mating behavior, circadian rhythm, and many other physiological functions of insects. In the present study, we performed RNA sequencing and de novo transcriptome assembly to uncover the GPCRs expressed in the stick insect Carausius morosus. The transcript assemblies were predicted for the presence of 7-transmembrane GPCR domains. As a result, 430 putative GPCR transcripts were obtained and 43 of these revealed full-length sequences with highly significant similarity to known GPCR sequences in …
Establishment Of Human Trabecular Meshwork Cell Cultures Usingnontransplantable Corneoscleral Rims, Kosala Dinuka Waduthanthri, Carlo Montemagno, Si̇bel Çeti̇nel
Establishment Of Human Trabecular Meshwork Cell Cultures Usingnontransplantable Corneoscleral Rims, Kosala Dinuka Waduthanthri, Carlo Montemagno, Si̇bel Çeti̇nel
Turkish Journal of Biology
Human trabecular meshwork (hTM) cell isolation in academic settings utilizes the motile nature of these cells, allowing them to migrate away from the explant and proliferate on distal regions of the culture substrate. Corneoscleral rims used for transplantation are a potential source of explants for the establishment of hTM cell cultures. However, cell isolation and the initiation of primary cell cultures from ocular tissues stored in Optisol-GS medium for an extended period of time (>6 days) has proven difficult, since Optisol-GS remarkably reduces cell viability and cellularity. Therefore, explants obtained from ocular tissues stored in Optisol-GS do not often …
Systematic Analysis Of The Frequently Amplified 2p15-P16.1 Locus Reveals Papolg As A Potential Proto-Oncogene In Follicular And Transformed Follicular Lymphoma, Deni̇z Kurşun, Can Küçük
Systematic Analysis Of The Frequently Amplified 2p15-P16.1 Locus Reveals Papolg As A Potential Proto-Oncogene In Follicular And Transformed Follicular Lymphoma, Deni̇z Kurşun, Can Küçük
Turkish Journal of Biology
Transformed follicular lymphoma (tFL) originates from histological transformation of follicular lymphoma (FL), which is the most common indolent non-Hodgkin lymphoma. High-resolution genomic copy-number analysis previously identified frequent amplification of the 2p15-p16.1 locus in FL and tFL cases. The genes (i.e. BCL11A, PAPOLG, PUS10, and USP34) in this amplified locus have not been systematically investigated to date in terms of their role in FL pathogenesis or transformation to tFL. Here we investigated the relationship between amplification and expression of genes in 2p15-p16.1 as well as their expression after histological transformation. NCBI GEO SNP array and gene expression profile (GEP) data of …
Overexpression Of A Fusion Defensin Gene From Radish And Fenugreek Improves Resistance Against Leaf Spot Diseases Caused By Cercospora Arachidicola And Phaeoisariopsis Personata In Peanut, Madhu Bala, Thankappan Radhakrishnan, Abhay Kumar, Gyan Prakash Mishra, Jentilal Ramjibhai Dobraia, Pulugurtha Bharadwaja Kirti
Overexpression Of A Fusion Defensin Gene From Radish And Fenugreek Improves Resistance Against Leaf Spot Diseases Caused By Cercospora Arachidicola And Phaeoisariopsis Personata In Peanut, Madhu Bala, Thankappan Radhakrishnan, Abhay Kumar, Gyan Prakash Mishra, Jentilal Ramjibhai Dobraia, Pulugurtha Bharadwaja Kirti
Turkish Journal of Biology
No abstract provided.
E2f6 Is Essential For Cell Viability In Breast Cancer Cells During Replication Stress, Inam Lafta
E2f6 Is Essential For Cell Viability In Breast Cancer Cells During Replication Stress, Inam Lafta
Turkish Journal of Biology
E2F6 is a member of the E2F family of transcription factors involved in regulation of a wide variety of genes through both activation and repression. E2F6 has been reported as overexpressed in breast cancers but whether or not this is important for tumor development is unclear. We first checked E2F6 expression in tumor cDNAs and the protein level in a range of breast cancer cell lines. RNA interference-mediated depletion was then used to assess the importance of E2F6 expression in cell lines with regard to cell cycle profile using fluorescence-activated cell sorting and a cell survival assay using (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide …
Proteomic And Physiological Responses In Mangrove Kandelia Candel Roots Under Short-Term High-Salinity Stress, Jianhong Xing, Dezhuo Pan, Lingxia Wang, Fanglin Tan, Wei Chen
Proteomic And Physiological Responses In Mangrove Kandelia Candel Roots Under Short-Term High-Salinity Stress, Jianhong Xing, Dezhuo Pan, Lingxia Wang, Fanglin Tan, Wei Chen
Turkish Journal of Biology
Kandelia candel is one of the mangrove species that are most resistant to environmental stress. As a typical nonsalt-secreting mangrove plant, K. candel is an ideal biological material to analyze the molecular mechanism of salt tolerance in woody plants. In this study, changes in protein abundance and expression profile in K. candel roots under high-salinity stress of 600 mmol L-1 NaCl were analyzed using isobaric tags for relative and absolute quantification (iTRAQ) assay. Moreover, the physiological parameters associated with metabolic pathways in which the differentially abundant proteins (DAPs) are involved were determined. A total of 5577 proteins were identified by …
Identification Of Streptomyces Strains Isolated From Humulus Lupulus Rhizosphere And Determination Of Plant Growth Promotion Potential Of Selected Strains, Fadi̇me Özdemi̇r Koçak
Identification Of Streptomyces Strains Isolated From Humulus Lupulus Rhizosphere And Determination Of Plant Growth Promotion Potential Of Selected Strains, Fadi̇me Özdemi̇r Koçak
Turkish Journal of Biology
In the present study it was aimed to obtain novel strains of actinomycetes that have the ability to promote plant growth. For this, seven soil samples from the rhizosphere of Humulus lupulus (Pazaryeri, Bilecik) were used and potential isolates were obtained. 16S rRNA genes of 30 isolates were amplified by PCR and sequenced. Eighteen isolates were found to be closely related to Streptomycetes spp. and they were tested for their proteolytic activity, cellulase activity, phosphate solubility, IAA production, biofilm formation, and growth in nitrogen-limited medium. Two isolates, HCI 12 and HCI 36, were selected according to the results of these …
Response Of Breast Cancer Cells To Ifnî±-2b In 2d And 3d Cell Cultures, Tetiana Herheliuk, Olena Perepelytsina, Andrij Ugnivenko, Lyudmila Ostapchenko, Mychailo Sydorenko
Response Of Breast Cancer Cells To Ifnî±-2b In 2d And 3d Cell Cultures, Tetiana Herheliuk, Olena Perepelytsina, Andrij Ugnivenko, Lyudmila Ostapchenko, Mychailo Sydorenko
Turkish Journal of Biology
The effect of IFNα-2b on the migration, proliferation, and expression of epithelial and mesenchymal markers of MCF-7 tumor adenocarcinoma cells in 2D and 3D cell cultures was examined. A significant cytostatic effect of IFNα-2b on the tumor population was detected. It was found that changes in the expression of epithelial (CKs and EpCAM) and mesenchymal markers were caused by changing the growth type of the tumor population. IFNα-2b inhibited migration of tumor cells to the suspension fraction and promoted an increase in expression of CK and EpCAM in 2D and 3D cell cultures, but only in the 3D culture was …
Transcriptome Analysis Reveals Differentially Expressed Genes Between Human Primary Bone Marrow Mesenchymal Stem Cells And Human Primary Dermal Fibroblasts, Eki̇m Z. Taşkiran, Beren Karaosmanoğlu
Transcriptome Analysis Reveals Differentially Expressed Genes Between Human Primary Bone Marrow Mesenchymal Stem Cells And Human Primary Dermal Fibroblasts, Eki̇m Z. Taşkiran, Beren Karaosmanoğlu
Turkish Journal of Biology
Stromal cells have been widely used in biomedical research and disease modeling studies in vitro. The most commonly used types of stromal cells are mesenchymal stem cells and fibroblasts. Their cellular phenotypes and differentiation capabilities are quite similar and there are no specific distinction criteria. In order to identify transcriptomic differences between these 2 cell types, we performed next-generation RNA sequencing. Using the global gene expression profile and pathway analysis, we showed that human primary bone marrow mesenchymal stem cells and human primary dermal fibroblasts have different molecular signatures. We also identified critical transcription factors that are differentially expressed between …
Genome And Metabolome Mining Of Marine Obligate Salinispora Strains To Discover New Natural Products, Süleyman Özakin, Ebru İnce Bostanci
Genome And Metabolome Mining Of Marine Obligate Salinispora Strains To Discover New Natural Products, Süleyman Özakin, Ebru İnce Bostanci
Turkish Journal of Biology
Marine microorganisms are receiving more attention as a promising potential source of new natural products. In the present study, we performed genomic and metabolomic analyses to explore the metabolic potential of the obligate marine actinomycete genus Salinispora. The genomes of thirty Salinispora strains were prospected in search of biosynthetic gene clusters including polyketide synthase (PKS), nonribosomal peptide synthetase (NPRS), terpene, indole, lantibiotics, and siderophores. We determined considerable diversity of natural product biosynthetic gene clusters in their genome. There were a total of 1428 putative gene clusters involved in the biosynthesis of various bioactive natural products. Furthermore, 1509 ketosynthase (KS) and …
Comparison Of Indigo Carmine Decolorization By Pseudomonas Aeruginosa And Crude Laccase Enzyme From Funalia Trogii, Fi̇li̇z Boran, Emre Bi̇rhanli, Özfer Yeşi̇lada, Eli̇f Özbey
Comparison Of Indigo Carmine Decolorization By Pseudomonas Aeruginosa And Crude Laccase Enzyme From Funalia Trogii, Fi̇li̇z Boran, Emre Bi̇rhanli, Özfer Yeşi̇lada, Eli̇f Özbey
Turkish Journal of Biology
The effects of incubation time, temperature, initial pH, and dye concentration on the indigo carmine decolorization activity of Pseudomonas aeruginosa ATCC 10145 and some factors on the decolorization potential of crude laccase enzyme obtained from Funalia trogii ATCC 200800 were comparatively investigated. This bacterium showed effective decolorization activity at all agitation and temperature values. Indigo carmine was greatly decolorized by P. aeruginosa at all pH values except pH 10. A decrease in decolorization activity occurred with increasing dye concentration, but this bacterium effectively decolorized the dye within 24 h. The decolorization process was through microbial metabolism, not biosorption. No decolorization …
Morphophysiological And Biochemical Response Of Mungbean [Vigna Radiata (L.) Wilczek] Varieties At Different Developmental Stages Under Drought Stress, Pooja Bangar, Ashok Chaudhury, Bhavana Tiwari, Sanjay Kumar, Ratna Kumari, Kangila Venkataramana Bhat
Morphophysiological And Biochemical Response Of Mungbean [Vigna Radiata (L.) Wilczek] Varieties At Different Developmental Stages Under Drought Stress, Pooja Bangar, Ashok Chaudhury, Bhavana Tiwari, Sanjay Kumar, Ratna Kumari, Kangila Venkataramana Bhat
Turkish Journal of Biology
The present study was conducted to assess the morphophysiological and biochemical responses during different developmental stages in mungbean varieties subjected to drought stress, and to screen the varieties for drought tolerance. A field experiment was performed according to a completely randomized design on 25 mungbean varieties with 3 replicates per variety. Stress treatment was applied at 3 levels: control (no stress), vegetative stage (25 days after sowing), and reproductive stage (35 days after sowing). According to combined analysis of variance, there were significant effects from drought stress on relative water content (RWC), membrane stability index (MSI), protein and proline content …
Development Of Antimicrobial Gelatin Films With Boron Derivatives, Sanem Argin, Merve Güleri̇m, Fi̇kretti̇n Şahi̇n
Development Of Antimicrobial Gelatin Films With Boron Derivatives, Sanem Argin, Merve Güleri̇m, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Food packaging technology has been advancing to provide safe and high quality food products and to minimize food waste. Moreover, there is a dire need to replace plastic materials in order to reduce environmental pollution. The aim of this study was to prepare biodegradable antimicrobial packaging films from gelatin. Boric acid, disodium octaborate tetrahydrate, and sodium pentaborate were incorporated as the antimicrobial agents. Films containing boric acid and its salts showed antibacterial effect against Staphylococcus aureus and Pseudomonas aeruginosa, as well as antifungal and anticandidal effects against Aspergillus niger and Candida albicans. The mechanical strength of the films was mostly …
Effects Of Notch Signalling On The Expression Of Sema3c, Hmga2, Cxcl14, Cxcr7, And Ccl20 In Breast Cancer, Cansu Küçükköse, Özden Yalçin Özuysal
Effects Of Notch Signalling On The Expression Of Sema3c, Hmga2, Cxcl14, Cxcr7, And Ccl20 In Breast Cancer, Cansu Küçükköse, Özden Yalçin Özuysal
Turkish Journal of Biology
Metastasis is the main reason for death in breast cancer. Understanding the molecular players in metastasis is crucial for diagnostic and therapeutic purposes. Notch signalling plays an oncogenic role in breast tumorigenesis and is involved in metastasis. Downstream mediators of Notch signalling in prometastatic processes are not yet fully discovered. Here we aimed to investigate whether Notch signalling regulates the expression of SEMA3C, HMGA2, CXCL14, CXCR7, and CCL20, which are involved in prometastatic processes, in breast cell lines. To this end, expression of the selected genes was analysed following Notch activation by overexpression of the Notch1 intracellular domain in the …
Complete Genome Sequence Analysis Of A Lytic Shigella Flexneri Vb_Sfls-Isf001 Bacteriophage, Khashayar Shahin, Majid Bouzari, Ran Wang
Complete Genome Sequence Analysis Of A Lytic Shigella Flexneri Vb_Sfls-Isf001 Bacteriophage, Khashayar Shahin, Majid Bouzari, Ran Wang
Turkish Journal of Biology
Shigellosis is one of the most important acute enteric infections caused by different species of Shigella, such as Shigella flexneri. Despite the use of antibiotic therapy to reduce disease duration, this approach is becoming less effective due to the emergence of antibiotic resistance among Shigella spp. Bacteriophages have been introduced as an alternative for controlling shigellosis. However, the bacteriophages must be without any lysogenic or virulence factors, toxin coding, or antibiotic-resistant genes. In this study, the whole genome sequence of vB_SflS-ISF001, a virulent Siphoviridae bacteriophage specific for Shigella flexneri, was obtained, and a comparative genomic analysis was carried out to …