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Articles 1651 - 1680 of 1681

Full-Text Articles in Biology

Nitrifying Bacterial Activity In South Eastern Black Sea(Coast Of Sürmene), Nüket Si̇vri̇, Hikmet Karaçam, Muzaffer Feyzi̇oğlu Jan 1998

Nitrifying Bacterial Activity In South Eastern Black Sea(Coast Of Sürmene), Nüket Si̇vri̇, Hikmet Karaçam, Muzaffer Feyzi̇oğlu

Turkish Journal of Biology

In this, some physico-chemical properties of seawater and their relationships with nitrification process were studied. Monthly water samples were taken at 9 stations (40^0 11' 19"-40^0 15' 47" E, 40^0 55' 26", 40^0 58' 00" N) along Sürmene coastline of South Eastern Black Sea during the period of December 1992 to August 1994. Temperature, dissolved oxygen, NO_3 - -N, NO_2 - -N and PO_4 -3 -P values were varied between 6.5-25.9^0C, 6.9-9.1 mg/l, 0.378-0.5 mg/l, 0.023-0.047 mg/l, 0.0055-0.0455 mg/l respectively. Most probable number of Nitrosococcus and Nitrobacter were 12-32 cell/100 ml and 12-29 cell/100 ml. There were significant relationships between …


Fine Structure Of A-Type Neurosecretory Cells In Abscisic Acid Treatedlocusta Migratorial. (Orthoptera), Önder Deveci̇ Jan 1998

Fine Structure Of A-Type Neurosecretory Cells In Abscisic Acid Treatedlocusta Migratorial. (Orthoptera), Önder Deveci̇

Turkish Journal of Biology

Fine structure of A-type neurosecretory cells was investigated in the adult males of Locusta migratoria in solitary phase. The cells were surrounded by glial cell processes and contained electron dense granules of 257 nm in an average diameter. Most of the granules were alongated but some were spherical. In control group insects, regular narrow channels of rough surfaced endoplasmic reticulum were evident in the peripheral parts of the cells. Golgi complexes were observed with electron dense material within its vacuoles and vesicles. The number of stored granules were not abundant and large empty areas were observed in the cells. In …


Effects Of Salt And Exogenous Putrescine On Seed Germination And Earlyseedling Growth Of Helianthus Annuusl. Var. Santafe, Fatma Teki̇n, Suna Bozcuk Jan 1998

Effects Of Salt And Exogenous Putrescine On Seed Germination And Earlyseedling Growth Of Helianthus Annuusl. Var. Santafe, Fatma Teki̇n, Suna Bozcuk

Turkish Journal of Biology

Effects of salt (NaCl) and putrescine (Put.) and various combinations of these (salt+Put.) on germination and some early growth parameters of sunflower ( Helianthus annuus L. var. Santafe) plants, grown under the controlled conditions, were investigated. Salt was used in 3 different con-centrations (50, 100, 200mM) and also, Put. was applied in 3 levels (0.01, 1, 2 mM). The salt treat-ment alone inhibited or delayed the seed germination, depending on concentration. Furthermore, some growth parameters (length of radicle, fresh and dry weights) studied in germination period were adversely affected by salt. In the treatment alone of Put., regardless of the …


The Effect Of 9450 Mhz Microwave Radiation On The Chromosomes Invivo, M. Zülküf Akdağ, Cemil Sert, M. Salih Çeli̇k, M. Emin Erdal, M. Aydın Ketani Jan 1998

The Effect Of 9450 Mhz Microwave Radiation On The Chromosomes Invivo, M. Zülküf Akdağ, Cemil Sert, M. Salih Çeli̇k, M. Emin Erdal, M. Aydın Ketani

Turkish Journal of Biology

In this study, we investigated whether microwave radiation affected chromosomal structure of rats or not. Adult male Spraque-Dawley rats were irradiated for 1 h a day to continous micorwave radiation (9450 MHz) at powever density 2.65 mW/cm 2 during 13. days. The control group were exposed to the some procedurus without microwave irradiation. On the following exposure day, rats were sacrificed under cetalar anesthesia, and their femurs were removed. Satandard bone marrow chromosome analysis were caried out, and experimental group was compared with control group. Our results showed that abnormal metaphase count increased and mitotic index decreasad, therefor, after and …


Transcription Of The Autographa Californicanuclear Polyhedrosis Virus P39 Gene In Bombyxmori Cell Culture, Zihni Demi̇rbağ Jan 1998

Transcription Of The Autographa Californicanuclear Polyhedrosis Virus P39 Gene In Bombyxmori Cell Culture, Zihni Demi̇rbağ

Turkish Journal of Biology

Transcription of the Autographa californica nuclear polynedrosis virüs (AcNPV; Family: Baculoviridae) p39 gene (delayed-early gene) has been studied extensively in the productive system of Spodoptera frugiperda cells. This is the first transcriptional study of this gene in the Bombyx mori cell line. In this study, comparative transcription of the AcNPV p39 gene was examined in S. frugiperda and B. mori cell cultures. Northern blot hybridization of totoal RNA samples, isolated from mock-infected (O and 48 hour post infection, h.p.i.) and AcNPV-infected (0, 2, 6, 12, 24, 48 h.p.i.) S. frugiperda and B. mori cell lines, was analyzed. Results indicates that …


Biochemical Characterization Of Elastase Frompseudomonas Aeruginosa Ses 938-1, Semra Kocabiyik, Ezgi Ergi̇n Jan 1998

Biochemical Characterization Of Elastase Frompseudomonas Aeruginosa Ses 938-1, Semra Kocabiyik, Ezgi Ergi̇n

Turkish Journal of Biology

We have investigated some biochemical properties of elastase from a new strain o f Pseudomonas aeruginosa (SES 938-1). Activities were measured at 410 nm using N-succinyl-L-(ala) 3 -p- nitroanilide as substrate. The elastase activity followed Michaelis-Menten kinetics over the substrate range of 0.067-0.540 mM with the apparent K m value of 0.375 mM. Optimum activity was observed at 36 °C and pH 7.5. elastase activity was inhibited by metal chelating agents and high concentrations of Mn 2+ , Zn 2+ and Ni 2+ . The results obtained suggest that elastase from P. aeruginosa SES 938-1 is a neutral metalloproteinase.


Concentrations Of Creatine Kinase And Myocardial Creatine Kinase Inrabbits Constituted Experimental Hyperthyroidism, İsmail Çeli̇k, Mehmet Kara, Eşref Yeği̇n, Halis Köylü Jan 1998

Concentrations Of Creatine Kinase And Myocardial Creatine Kinase Inrabbits Constituted Experimental Hyperthyroidism, İsmail Çeli̇k, Mehmet Kara, Eşref Yeği̇n, Halis Köylü

Turkish Journal of Biology

In this study, 20 male (1500±50 g) white, New Zealand strain rabbits ( Oryctolagus cuniculus huxley) jere used. Of these ten were used for experimental group, and the others were used controls. To get hyperthyroidism in the experiment animals, L-Thyroxin 50 µg/kg of body weight daily for two weeks, was injected to the animals by intravenous. The control group rabbits were injected only 0.9% isotonic salt water which L-Thyroxin dissolved in it. The levels of total ceratine kinase (CK) and cardirac creatine kinase (CK-MB) were recorded as 1079 ± 597 U/L and 879 ± 128 U/L in control group whereas …


The Effects Of Copper Chloride On The Gene Activity Of The Salivarygland Polytene Chromosomes Of Drosophila Melanogaster, Sevgi Levent, Handan Uysal, Zafer Bahçeci̇ Jan 1998

The Effects Of Copper Chloride On The Gene Activity Of The Salivarygland Polytene Chromosomes Of Drosophila Melanogaster, Sevgi Levent, Handan Uysal, Zafer Bahçeci̇

Turkish Journal of Biology

The effects of copper chloride on the salivary gland polytene chromosomes of third instar larvae of Drosophila melanogaster have been investigated. Copper chloride have been applied to the larvae in vivo. It has been shown that different band sections of the salivary gland chromosomes become puffed and many developmental puffs were inactivated. As a result of the copper chloride treatment, band 22A, 23EF, 27E, 28B, 35F on chromosome arm 2L; 47A, 54BD, 55DE, 56DE, 58CF, 59B on chromosome arm 2R; 62A, 62DE, 68BC, 70DE, 73B, 74D on chromosome arm 3L have become puffed. The average size of these puff have …


Effects Of Carbohydrates On Levels Of Total Glycogenand Protein In Adult Female Pimpla Turionellae L., Pınar Özalp, İskender Emre Jan 1998

Effects Of Carbohydrates On Levels Of Total Glycogenand Protein In Adult Female Pimpla Turionellae L., Pınar Özalp, İskender Emre

Turkish Journal of Biology

Effects of 22 carbohydrates belonging to various groups on the levels of total glycogen and protein in the adult female Pimpla turionellae L. were investigated. Sucrose was used as control in all experiments. Among the tested carbohydrates, xylose, ribose, rhamnose, mannose, maltose, cellobiose, melesitose, raffinose, glycogen, dulcitol and mannitol caused significant decreases on total glycogen level, but arabinose, fructose, galactose, glucose, sorbose, lactose, melibiose, trehalose, starch, sorbitol and a-methyl-d-glucoside did not any significant effect. The levels of total protein of the insect was increased by xylose, while it was decreased by glucose. The other carbohydrates tested did not cause any …


The Targeting Mechanisms Of Membrane Proteins, Nihat Di̇lsi̇z Jan 1998

The Targeting Mechanisms Of Membrane Proteins, Nihat Di̇lsi̇z

Turkish Journal of Biology

This review describes what is known about the way of insertion and translocation of membrane proteins and the factors that determine these structures. The translocation of proteins across the cytoplasmic membrane in prokaryotes and that across the endosplasmic reticulum membrane in eukaryotes are similar in that both require the sinyal peptide that is attached to the amino terminus of the proteins. In general, mebrane proteins reach their final target via the secretory (Sec) pathway which are called Sec-dependent proteins. However, it has become clear that a few membrane proteins are not involved in Sec-pathway and these are called Sec-independent proteins.


The Determination Of Biological Activities Of Indole-3-Acetic Acid (Iaa),Gibberellic Acid (Ga_3 ), Abscisic Acid (Aba) And Zeatin Obtained Fromphanerochaete Chrysosporium Me446, Serpil Ünyayar, S. Fatih Topcuoğlu Jan 1998

The Determination Of Biological Activities Of Indole-3-Acetic Acid (Iaa),Gibberellic Acid (Ga_3 ), Abscisic Acid (Aba) And Zeatin Obtained Fromphanerochaete Chrysosporium Me446, Serpil Ünyayar, S. Fatih Topcuoğlu

Turkish Journal of Biology

In this study, the biological activities of indole-3-acetic acid (IAA), gibberellic acid (GA_3 ), abscisic acid (ABA) and zeatin obtained from the culture medium and mycelia of Phanerochaete chrysosporium ME446 were determined. With this aim, oat coleoptile growth test for IAA and ABA and rice germination test for GA_3 and chlorophyl amount test for zeatin were used. At the end of biological tests IAA, ABA and zeatin were observed to have full biological activity, whereas GA 3 has only partial biological activity.


The Changes Of The Localization Of Ascorbic In The Rat Adrenalgland On Microwave Applied, Yusuf Nergi̇z, M. Aydın Ketani, Zülküf Akdag, M. Salih Çeli̇k, Ö. Mukaddes Şahan Jan 1998

The Changes Of The Localization Of Ascorbic In The Rat Adrenalgland On Microwave Applied, Yusuf Nergi̇z, M. Aydın Ketani, Zülküf Akdag, M. Salih Çeli̇k, Ö. Mukaddes Şahan

Turkish Journal of Biology

The purpose of this study was to investigate the effect of microwave on the localization of ascorbic acid in the rat adrenal gland. We studies on 21 male Spraque-Dawley rast (220-250 g), aged 2.5 month. The animals were divided into three different groups. First group was taken on control (n:7). The second group (n:7) 9450 MHz, 2.65 mW/cm 2 was applied for one hour a day for 13 days, while in the third group (n:7) this application continued for 52 days. Rast were sacrified under ether anaesthesia and then their adrenal gland were removed enbloc and fixed in silver nitrate …


Carotenoid Pigments Stabilization In The Fillet Of Rainbow Trout(Oncorhynchus Mykiss) During Frozen Storage, Mahmut Yanar, Mehmet Çeli̇k, Yasemen Yanar, Metin Kumlu Jan 1998

Carotenoid Pigments Stabilization In The Fillet Of Rainbow Trout(Oncorhynchus Mykiss) During Frozen Storage, Mahmut Yanar, Mehmet Çeli̇k, Yasemen Yanar, Metin Kumlu

Turkish Journal of Biology

In this study, changes in the total carotenoid content of the fillet of rainbow trout, pigmented with astaxanthin supplemented diet, stored at -20ºC, in vacuum packaging for 2, 4 and 8 month periods were inverstigated. Total carotenoid concentration of the fillets did not change during the first two month storing period but the loss was 1.58 % and 3.58 for the 4 th and 8 th months, respectively. Yet, these changes were not signigicantly different than the level measured at the beginning of the experiment (P>0.05). It is concluded that carotenoid losses from the flesh do not affect the …


The Effect Of Some Plant Materials On The Grwth And Productivity Ofpleurotus Ostreatus (Jacq. Ex. Fr.) Kum. Var. Salignus (Pers. Ex. Fr.)Konr. Et Maubl., Abdunnasır Yildiz, Ramazan Demi̇r Jan 1998

The Effect Of Some Plant Materials On The Grwth And Productivity Ofpleurotus Ostreatus (Jacq. Ex. Fr.) Kum. Var. Salignus (Pers. Ex. Fr.)Konr. Et Maubl., Abdunnasır Yildiz, Ramazan Demi̇r

Turkish Journal of Biology

In this study, the soybean, sorghum, peanut and wheat straw were used as culture media for Pleurotus ostreatus var. salignus. Mycellium growth period, basidiocarp formation period anda first, second, third and fourth harvesting periods were determined as; 10.0, 24.3, 28.6 38.6, 47.3 and 58.6 days respectively as the shortest periods found with peanut straw; and 22.6, 52.6, 56.6, 68.6, 73.6 and 88.6 days respectively, as the longest periods found with sorghum straw. The fresh mushroom yield obtained with 100 g material (70 % moisture) at first, second, third and fourth harwesting periods and total yield was 8.6, 8.1, 4.8, 3.4 …


Fatty Acid Composition Of Agaricus Bisporus (Lange) Sing., Abdurrahman Aktümsek, Celâleddin Öztürk, Giyasettin Kaşik Jan 1998

Fatty Acid Composition Of Agaricus Bisporus (Lange) Sing., Abdurrahman Aktümsek, Celâleddin Öztürk, Giyasettin Kaşik

Turkish Journal of Biology

Fatty acid compositions of fruit body, stem, lamellae and total of Agaricus bisporus were seperately analysed by GLC. In the all fatty acid compositions of A. bisporus, linoleic acid were predominant. Percentages of linoleic acid were varied between 53.45 - 68.78%. It was showed that the other major fatty acids were palmitic, oleic and stearic acid in the fatty acid compositions.


The Algal Flora Of The Littoral Region In Lake Akşehir, Ayşe Elmaci, Olcay Obali Jan 1998

The Algal Flora Of The Littoral Region In Lake Akşehir, Ayşe Elmaci, Olcay Obali

Turkish Journal of Biology

In this study, composition and seasonal variations of littoral algae in Lake Akşehir were investigated from June 1992- November 1993. In the littoral region, the algal flora were composed of 115 species belonging to Bacillariophyta, Charophyta, Chlorophyta, Cyanophyta, Euglenophyta. Bacillariophyta was predominant in respect to species diversity and density. Cyanophyta and Chlorophyta were in minority. In general, the most of the identified species of the algal flora of Lake Akşehir are considered as the members of eutrophic lakes which are rich in nutritional salts.


The Effects Of Trimethoprim On The Chromosomal Aberrations On The Radiological Techniciances Who Smoke, Etem Akbaş, Turgay Budak Jan 1998

The Effects Of Trimethoprim On The Chromosomal Aberrations On The Radiological Techniciances Who Smoke, Etem Akbaş, Turgay Budak

Turkish Journal of Biology

Trimethoprim, cigerette smoking and radition is known to have a clastogenic effects on chromosomes when applied to oneby one. In this study all of this aggents have been applied together. 8, 35 and 60 \mug/ml doses of trimethoprim applied to the bloods which were taken from five healthy radiology technicians who are cigarette smokers, for the periods of 6, 24 and 48 hours. The same quantity of culture medium was added to the cultures which were separated for control in the same period. For each dose-aplication ;eriod combination, from each individual 50 metaphases were evaluated in relation to the ratio …


Antimicrobial Activity Of The Lichen Cetraria Islandica (L.) Ach., Başaran Dülger, Fahrettin Güci̇n, Ali Aslan Jan 1998

Antimicrobial Activity Of The Lichen Cetraria Islandica (L.) Ach., Başaran Dülger, Fahrettin Güci̇n, Ali Aslan

Turkish Journal of Biology

In this study, extracts of Cetraria islandica (L.) Ach. were prepared with Ethyl acetate, Acetone, Chloroform and Ethanol and antimicrobial activities of these extracts were examined on test microorganisms as follows: Escherichia coli ATCC 11230, Enterobacter aerogenes CCM 2531, Staphylococcus aureus 6538P, Staphylococcus epidermidis, Bacillus subtilis La2114, Bacillus cereus var. mycoides, Bacillus sphaericus, Bacillus thurigiensis, Bacillus megaterium, Mycobacterium smegmatis RUT, Salmonella thyphimurium, Candida utilis La991, Candida albicans CCM314, Aspergillus flavus, Aspergillus oryzae, Aspergillus fumigatus, Trichophyton rubrum, Botrytis cineriae, Fusarium oxysporium, Streptomyces murinus ISP 5091 and Nocardia cornea IFO 14403 by Disc Diffusion Methods. As a result of study, we have …


The Investigation Of Antimicrobial Effect Of Some Plant Extract, Ahmet Ilçim, Metin Diğrak, Eyüp Bağci Jan 1998

The Investigation Of Antimicrobial Effect Of Some Plant Extract, Ahmet Ilçim, Metin Diğrak, Eyüp Bağci

Turkish Journal of Biology

The Parmelia furfuracea (L) Zopf. (Liken), Crocus chrysanthus (Herbert) Herbert (Çigdem), Rumex scutatus L., (Kuzukulagi), Myrtus communis L. subsps. communis Ic: Sibth. and Sm. (Mersin), Asphodelus aestivus L. (Çiris) Eugenia caryophyllata Thunb. (Karanfil) which were collected from Kahramanmaras and Hatay region have been investigated in respect of their antimicrobial and antifungal activities against different test organisms ( Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus, brevis FMC 3, Escherichia coli DM, Klebsiella pneumoniae FMC 5, Enterobacter aerogenes CCM 2531, Pseudomonas aeruginosa DSM 50071, Staphylococcus aureus COWAN 1, Listeria monocytogenes SCOTT A, bacteria and Candida albicans CCM 314 and Saccharomyces …


Effects Of Various Proportions Of Different Additive Substances On Themiselle Growth Of Pleurotus Florida Fovose, On Druationof Formation And Development Of Basidiocarps And On The Yield, Abdunnasır Yildiz Jan 1998

Effects Of Various Proportions Of Different Additive Substances On Themiselle Growth Of Pleurotus Florida Fovose, On Druationof Formation And Development Of Basidiocarps And On The Yield, Abdunnasır Yildiz

Turkish Journal of Biology

Into aliment place that we prapered for vegetative propagation of the miscell, in 1 lt pure water 10 g malt-extract, 0.2 g K 2 HPO 4 , 0.1 g CaCl 2 .2H 2 O 0.05 g MgSO 4 . 7H 2 O, and g (NH 4 )SO 4 as well as 15 g agar, barley broken and wheat flour in 10, 15 and 20 g doses were added as contribution substance (contribution substance, however, weren't added to the control group). In petri boxes, it has been identified that P. florida miscelles grafted for aliment-agar medium covered the medium in 8.6 …


In Vivo Dna Methylation Of Escherichia Colidh5\Alphaand Top10f' Strains By Bacterial Cytosine-5methyltransferase M. Msp1, Öztürk Özdemi̇r, David Hornby Jan 1998

In Vivo Dna Methylation Of Escherichia Colidh5\Alphaand Top10f' Strains By Bacterial Cytosine-5methyltransferase M. Msp1, Öztürk Özdemi̇r, David Hornby

Turkish Journal of Biology

At the chromatin level, methylated CpG dinucleotides are R.Msp1 resistant compared with nonmethylated counterparts. The DNA of two E.coli strains was analyzed following transformation-with bacterial cytosine-5-methyltransferase gene M.Msp1 in mammalian transfection vector pcDNA3. Expression of the M.Msp1 was tested by R.Msp1 digestion. The results suggest that the DNA of both strains was fully methylated at the CCGG sequences, by the active enzyme under the control of T7 and cytomegalovirus (CMV) promoters. Methylated DNA can not be digested and it exhibits higher frag-ment sizes in 1% agarose gel in contrast to the untransformed cell DNAs in vivo.


The Construction Of A Mammalian Transfectionvector For Expression Of Cytosine-5 Specific Dnamethyltransferase Gene M.Msp1 In Cultured Cells, Öztürk Özdemi̇r Jan 1998

The Construction Of A Mammalian Transfectionvector For Expression Of Cytosine-5 Specific Dnamethyltransferase Gene M.Msp1 In Cultured Cells, Öztürk Özdemi̇r

Turkish Journal of Biology

The expression vectors are designed for expression and purification of normal or recombi-nant gene of interest. There is a wide variety of stable and transferable selectable mammalian expres-sion vectors.The vector pCDM8 and its derivatives, pcDNAI/Ampicilline are widely used for cloning and analyzing of genes in higher eukaryotic cells. The vectors pRC/CMV, pcDNA3 and pRC/RSV are designed for high-level expression of recombinant genes in mammalian cells. In the present study, we have been able to transfect and express the monospecific bacterial (Moroxcella spp) cytosine-5 DNA methyltransferase (MTase) M.Msp1 gene in cultured cells within a newly con-structed mammalian transfection vector. We have …


Determination Of Heavy Metal Levels In Some Mossspecies Around Thermic Power Stations, Özlem Tonguç Jan 1998

Determination Of Heavy Metal Levels In Some Mossspecies Around Thermic Power Stations, Özlem Tonguç

Turkish Journal of Biology

In our studry, the chemical analysis related to the heavy metal pollution in some mosses grown near the thermic power stations which built close to Mugla and its environs, was carried out.


Serological Properties Of Pseudomonas Syringaepvphaseolicolaisolates Collected From Eskisehir, Dilek Ertugrul, Kıymet Güven Jan 1998

Serological Properties Of Pseudomonas Syringaepvphaseolicolaisolates Collected From Eskisehir, Dilek Ertugrul, Kıymet Güven

Turkish Journal of Biology

Thirty six bean seed samples collected from bean fields in Eskisehir-Türkiye were examined for detection and isolation of Pseudomonas syringae pv. phaseolicola. A polyclonal antiserum specific to P. s. pv. phaseolicola NCPPB 52 was raised in a rabbit and both biochemical and serological test were used for identification. Heat-stable O antigens of 92 isolates identified as P. S. pv. phaseolicola together with authentic isolates of the bacterium were compared by Ouchterlony immunodiffusion test and all the isolates had a common antigenic structure.


The Effects Of Phenoxybenzamine And Cold Stresson Tyrosine Hydroxylase Enzyme Activity Andexpression Of Tyrosine Hydroxylase Gene In The Ratadrenal Medulla, Muhittin Yürekli Jan 1998

The Effects Of Phenoxybenzamine And Cold Stresson Tyrosine Hydroxylase Enzyme Activity Andexpression Of Tyrosine Hydroxylase Gene In The Ratadrenal Medulla, Muhittin Yürekli

Turkish Journal of Biology

The effects of cold exposure and cold plus phenoxybenzamine treatment on tyrosine hydrox-ylase (TH) activity and TH m RNA levels were investigated in the adrenal medulla of Sprague Dawley (SD) rats. SD rats were exposed to cold (4 °C) for 48 hours. Phenoxybenzamine was injected intra-peritoneally (i.p) at 20 mg/kg, which was prepared in 5% ethanol containing 0.9 % NaCL TH activity was measured by monitoring the formation of 3 H 2 O as a product of L-(3 H)-tyrosine hydroxylation, the formation of L-(3 H)-dopa from L-(3 H)-tyrosine. Total adrenal RNA was isolated and blotted onto a nylon membrane and …


A Comparison Of Germ Tube Production By Candidaalbicans In Three Media, Gülhan Vardar-Ünlü Jan 1998

A Comparison Of Germ Tube Production By Candidaalbicans In Three Media, Gülhan Vardar-Ünlü

Turkish Journal of Biology

The conversion of C. albicans. from commensal to the pathogenic state is associated with its ability to make the dimorphic transition from the yeast to the hyphal (germ tube) form. Three liq-uid media were compared in ability to promote germ tube production by C. albicans. Medium 199 and Glucose-Glycine Medium at 37°C were highly satisfactory and gave 90 % conversion of yeast cells to hyphal-form cells. Sucrose-Gelatine medium gave only 15% conversion. Glucose-Glycine medium appeared to give more rapid germ tube production than 199 Medium.


Determination Of 5'-Nucleotidase Activity Of Higherplants By Using An Improved Method, Yusuf Turan Jan 1998

Determination Of 5'-Nucleotidase Activity Of Higherplants By Using An Improved Method, Yusuf Turan

Turkish Journal of Biology

The presence of relatively high non-specific phosphatase activities are known in various plant tissues. These enzymes also catalyze the removal of inorganic phosphate from nucleoside monophosphates. According to published reports, so far, the experimental investigations, concerning the 5'-nucleotidase activity, have been mostly carried out without consideration of the non-specific phosphatase activities in crude enzymic extracts. However, few researchers have been included some inhibitors of these phosphatases, such as NaMoO 4 or KF, in assay mixtures. But phosphatases are not completely inhibited by these inhibitors and, therefore, interfere the activity of 5'-nucleotidase. Thus, the non-specific phosphatase activities were determined in crude …


Purification And Characterization Of Pediocin F, Abacteriocin Produced By Pediococcus Acidilacticif, Özlem Osmanagaoğlu, Ufuk Gündüz, Yavuz Beyatli, Cumhur Çökmüş Jan 1998

Purification And Characterization Of Pediocin F, Abacteriocin Produced By Pediococcus Acidilacticif, Özlem Osmanagaoğlu, Ufuk Gündüz, Yavuz Beyatli, Cumhur Çökmüş

Turkish Journal of Biology

In this work, Pediococcus acidilactici F isolated from fermented sausage was reported to produce an extracellular antimicrobial peptide, termed Pediocin F, that was protein in nature and effec-tive against many bacteria associated with food spoilage and food related health hazards. Pediocin F is reported here to be sensitive to proteolytic enzymes, resistant to heat and organic solvents, and active over a wide range of pH. We have also reported that the extracellular antimicrobial protein pro-duced by P. acidilactici F was really a small peptide. Coomassie-blue stained SDS-PAGE technique allowed us to estimate the molecular weight of this small peptide to …


A Theoretical Study On The Sex-Pheromones Of Therice Moth, Corcyra Cephalonica Stainton, Lemi Türker Jan 1998

A Theoretical Study On The Sex-Pheromones Of Therice Moth, Corcyra Cephalonica Stainton, Lemi Türker

Turkish Journal of Biology

Molecular orbital calculations (AM1 type) were performed on the geometry optimized structures of various farnesal isomers and methyl esters of farnesoic acids. It has been found that the major component, E, E-farnesal, of the sex-pheromone for the rice moth, Corcyra cephalonica Stainton is chemically the most exothermic and stable of all the farnesal isomers. Then, the resem-blance of molecular shapes was detrmined in order to design more stable sex-attractants.


Larval Growth And Survival Of Penaeus Indicus(Decapoda: Penaeidae) On Live Feeds, Metin Kumlu Jan 1998

Larval Growth And Survival Of Penaeus Indicus(Decapoda: Penaeidae) On Live Feeds, Metin Kumlu

Turkish Journal of Biology

This study was conducted to compare the suitability of three microalgal species (Tetraselmis chuii, Skeletonema costatum and Rhinomonas reticulata), both individually and in combination, as food for Penaeus indicus during larval culture. To determine the best feeding density, each alga was fed to protozoea 1 (PZI) larvae from 10 to 80 cells mL -1 . The best algal-cell density, promoting the highest survival rate, greatest growth and fastest larval development, was obtained at 60-70 cells mL -1 with a combination of T. chuii and S. costatum. Low cell densities (10-20 cells mL -1 ) were not effective. The diatom S. …