Biology Commons^™

Integrated Pest Management In The Academic Small Greenhouse Setting: A Case Study Using Solanum Spp. (Solanaceae)., Daniel S. Hayes, Ingrid E. Jordon-Thaden, Jason T. Cantley, Angela J. Mcdonnell, Christopher T. Martine

Faculty Journal Articles

Premise

Botanical faculty and staff at academic institutions are often tasked with establishing and/or caring for plant collections held in small greenhouse facilities. Once plants are in place, an especially acute challenge is managing plant pest/pathogen populations. Integrated pest management (IPM) approaches are an excellent option, but few examples exist in the literature of successful programs that have been developed in academic small greenhouse settings.

Methods and Results

Over several years, we developed an IPM program for two small research greenhouses on the campus of a primarily undergraduate institution where hundreds of plants have been grown for studies in the …

Species Composition, Diversity And Distribution Of Vascular Epiphytes In The Kihansi Gorge Forest, Tanzania, Hashim Mangosongo, Martin Griffiths

Tanzania Journal of Science

This study aimed at assessing the species composition, diversity and distribution of vascular epiphytes in the Kihansi Gorge Forest. Epiphytes sampling was conducted in twenty sampling plots established in the strip of riverine forest within the Kihansi Gorge. All vascular epiphytes growing on trees (phorophytes) rooted in the plots were sampled using ground-based technique as the method of access to the tree canopies.  A total of 476 epiphyte individuals belonging to 20 species and 10 families were recorded in the study area. Among the twenty species recorded, Drynaria laurentii was the most abundant and most widely distributed in the study …

Morphological And Molecular Identification Of Pratylenchus Goodeyi Associated With Banana In Tanzania, Doreen Mgonja, Gladness Temu, Magreth Mziray, Beatrice Kashando, Ambilikile Mwenisongole, Minza Masunga, Sylvester Lyantagaye4, Nessie Luambano

Tanzania Journal of Science

Banana (Musa sp. [L]) is an important staple food and cash crop for about 30 percent of the total population in Tanzania. Root lesion nematodes, particularly Pratylenchus goodeyi, are among the major pests affecting banana production in Tanzania. Morphological, morphometric and molecular approaches are required for accurate identification of nematodes. In this study, 80 P. goodeyi isolates from major banana growing areas in Tanzania were identified. Sequence analysis of part of the small subunit (SSU), large subunit (LSU) and internal transcribed spacers (ITS) regions of ribosomal DNA (rDNA) confirmed the identity of P. goodeyi. DNA sequences were analysed using Geneious …

Seed And Seedling Data From Sugarcreek Metropark Restoration Experiment, Michaela J. Woods, Meredith Cobb, Ryan W. Mcewan

Five Rivers MetroParks Collaboration Data Archive

This dataset encompasses information following seed germination and seedling growth of three tree species: Quercus rubra, Juglans cinerea, and Carya laciniosa. Seed sizes were recorded prior to incubation in sand, vermiculite, or without media. Seeds were then germinated with time to germination recorded in this dataset. After germination, seedlings were planted in Sugarcreek Metropark in either fall 2011 or spring 2012. One-half of seedlings were planted in tree tubes and a half without. Seedling height and diameter was recorded in June 2014 and March 2019, and death of seedlings was noted.

Pollinator Networks In Established Urban Prairies Compared To Rural Remnant Prairies, Amanda Lynn Coleman

Graduate Theses/Dissertations

Prairies support over 800 species of plants, insects, birds, fish, and mammals, even though only 1% of remnant prairies remain in the United States. Importantly, urban prairie “gardens/plots” are gaining popularity for their ecological services. However, it is not known to what extent these small urban prairies can sustain the plant-pollinator interactions that are vital to both the insects and the plants. The goal of my research was to examine plant/pollinator interactions in three urban prairies in southwest Missouri and compare them to rural prairies because rural prairies were predicted to have stronger plant/pollinator networks. Rural units were: Woods Prairie, …

Seed Predation By Owyhee Harvester Ants And The Potential Of Seed Introductions In Recovery Efforts For Slickspot Peppergrass, Jennifer A. Brown

Boise State University Theses and Dissertations

Slickspot peppergrass (Lepidium papilliferum) is a rare plant endemic to the sagebrush-steppe habitat in southwestern Idaho. Within sagebrush-steppe, the plant is restricted to microsites known as “slick spots”– shallow depressions of soil characterized by distinct clay layers and surface water retention that is higher than that of surrounding areas. Having one of the highest extirpation rates among rare plant taxa in Idaho, and considering its unique habitat requirements, limited range, and declining numbers, land managers and conservationists have voiced concern regarding the species’ long-term viability. While range-wide declines in slickspot peppergrass have been attributed largely to the loss …

Sequence Analysis Of The Ren1 Genomic Region From The Grapevine ‘Kishmish Vatkana’, Dani Joseph

Graduate Theses/Dissertations

The REN1 region of the grapevine ‘Kishmish Vatkana’ was mapped as the locus that confers resistance to the economically important disease, grape powdery mildew. The purpose of this work was to extend the nucleotide sequence information of this region. By sequencing a heretofore unknown bacterial artificial chromosome clone, the sequence information of this region was extended by 46,890 nucleotides. Sequencing was performed using the third-generation sequencing method, named Oxford Nanopore Technology (ONT). In order to improve the accuracy of the sequence data, a modified ONT library preparation method was developed. ONT sequencing of a library prepared with the modified protocol …

Photosynthetic Response Of Pentagramma Triangularis To Summer Desiccation, Brandon R. Grinovich, Stephen D. Davis

Featured Research

Ferns are thought to have been some of the first vascular plants to develop, perhaps on the order of 390 million years ago. Since then, ferns have speciated and developed innovative methods of survival especially in relation to water stress. Research conducted in 2016 by Helen Holmlund showed that eight California fern species fell into four primary classes of survival strategies, one of those being resurrection. Pentagramma triangularis is one of the resurrection-type ferns and is endemic to the western coast of the United States. As P. triangularis desiccates during the summer it enacts a physiological response by which it …

Effects Of Invasive Species On Post-Fire Ceanothus Spinosus Seedling Establishment, Nina Duchild, Frank W. Ewers, Stephen D. Davis

Featured Research

This study compared seedling establishment of the native chaparral species Ceanothus spinosus in two different burn sites at Pepperdine University following the Woolsey Fire of November 2018. One site was a low-density fuel site, and experienced a “cool burn,” and the other site was a high-density fuel site and experienced a “hot burn.” In the low-density fuel site, fire type-conversion had occurred, resulting in a dominance of exotic species. The high-density fuel site was populated by many native fire-following species and chaparral shrubs, exemplifying pristine post-fire conditions. Initially, I hypothesized that seedling success in the cool burn site would be …

Seedling And Resprout Survival Of Three Chaparral Species Following Woolsey Wildfire, Karagan L. Smith, Stephen D. Davis

Featured Research

Fire is a common natural disaster that effects Southern California. Combined with recent chronic drought, there has been an increase in the damage and frequency of these fires in recent years. Three chaparral species are common to the Pepperdine campus: Malosma laurina, Ceanothus spinosus, and Ceanothus megacarpus. The survival of these native species is essential because they are indicators for the changes that are occurring in our local ecosystem and provide further implications of how our ecosystem responds to natural disaster. Seedling recruitment and resprouting are mechanisms these chaparral species use to reestablish postfire. Postfire data have been recorded …

The Impact Of The Woolsey Fire On The Fungus Botryosphaeria Dothidea, Juan Arguijo

Featured Research

Southern California suffered a six-year drought in 2012 through 2018 during which the laurel sumac, Malosma laurina, experienced water stress and the fungus Botryosphaeria dothidea began to kill this plant, causing a massive dieback of this keystone chaparral species in the Santa Monica Mountains. Botryosphaeria is an opportunistic endophytic fungus that invades the xylem of woody type plants and waits till the plant starts to experience stress to proliferate. In November of 2018, the Woolsey fire burned up to 96,000 acres of vegetation. I ran tests at two sites on the Pepperdine University campus, the Baxter Ridge Site which …

Post-Fire Soil Microbiome Recovery And Respiration In A Chaparral Ecosystem, Mari R. Irving, Stephen D. Davis

Featured Research

This study characterizes post-fire recovery of microbial and fungal populations adjacent to three keystone chaparral plant species affected by varying burn intensities via systematic measurement of soil respiration. The CO₂ released from soil surrounding Ceanothus spinosus, Heteromeles arbutifolia and Malasma laurina affected by mild, severe or no heat during the Woolsey Fire of November 2018 were monitored and used as an indicator of microbial, fungal, and root activity. We hypothesized that soil which experienced less intense heat would exhibit higher rates of respiration based on preliminary data taken in March 2019. However, the opposite was observed from May through …

Getting To The Root Of Selenium Hyperaccumulation—Localization And Speciation Of Root Selenium And Its Effects On Nematodes, Christine N. Prins, Laura J. Hantzis, Jose R. Valdez Barillas, Jennifer J. Cappa, Sirine C. Fakra, Cecilia Milano De Tomasel, Diana H. Wall, Elizabeth A. H. Pilon-Smits

Biology Faculty Publications (Archived)

Elemental hyperaccumulation protects plants from many aboveground herbivores. Little is known about effects of hyperaccumulation on belowground herbivores or their ecological interactions. To examine effects of plant selenium (Se) hyperaccumulation on nematode root herbivory, we investigated spatial distribution and speciation of Se in hyperaccumulator roots using X-ray microprobe analysis, and effects of root Se concentration on root-associated nematode communities. Perennial hyperaccumulators Stanleya pinnata and Astragalus bisulcatus, collected from a natural seleniferous grassland contained 100–1500 mg Se kg−1 root dry weight (DW). Selenium was concentrated in the cortex and epidermis of hyperaccumulator roots, with lower levels in the stele. The …

Effects Of Reduced Ph On Health Biomarkers Of The Seagrass Cymodocea Nodosa, Regan A. Mcenaney

DePaul Discoveries

Ocean acidification is a growing problem that may affect many marine organisms in the future. Within 100 years the pH of the ocean is predicted to decrease to 7.8, from the current ocean pH of around 8.1. Using phenolic acid levels as a stress indicator as well as respiration and chlorophyll content as a measure of health, the effect of lowering pH was tested on the seagrass, Cymodocea nodosa, in a controlled environment. Plant samples, water, and soil were taken from the Bay of Cádiz, Spain, and placed in aquaria in a temperature-controlled room. One control group was left …

On The Composistion Of Cymodocea Nodosa Root Exudate Under Artificial Blue, Green And Natural Light Conditions, Armand Martin, Ben Manriquez, Christian Pompa, Aaron Saper, Kyle A. Grice, Jason Bystriansky

DePaul Discoveries

Seagrasses are identified as a sentinel species: a good indicator of overall marine ecosystem health and function. At the rhizome, they are known to interact with marine bacteria by exchanging energy in the form of glucose and free amino acids secreted through root exudate in exchange for microbe-fixated nitrogen that can be utilized for plant growth. To analyze potential outcomes of possible future changes in light availability, an experiment was designed to collect and analyze the root exudate of Cymodocea nodosa under three light conditions (standard fluorescent light, blue LED, and green LED light). After 72 hours of treatment, the …

Genome Of The Tropical Plant Marchantia Inflexa: Implications For Sex Chromosome Evolution And Dehydration Tolerance, Rose A. Marks, Jeramiah J. Smith, Quentin Cronk, Christopher J. Grassa, D. Nicholas Mcletchie

Biology Faculty Publications

We present a draft genome assembly for the tropical liverwort, Marchantia inflexa, which adds to a growing body of genomic resources for bryophytes and provides an important perspective on the evolution and diversification of land plants. We specifically address questions related to sex chromosome evolution, sexual dimorphisms, and the genomic underpinnings of dehydration tolerance. This assembly leveraged the recently published genome of related liverwort, M. polymorpha, to improve scaffolding and annotation, aid in the identification of sex-linked sequences, and quantify patterns of sequence differentiation within Marchantia. We find that genes on sex chromosomes are under greater …

Solanum Plastisexum, An Enigmatic New Bush Tomato From The Australian Monsoon Tropics Exhibiting Breeding System Fluidity., Angela J. Mcdonnell, Heather B. Wetreich, Jason T. Cantley, Peter Jobson, Christopher T. Martine

Faculty Journal Articles

A bush tomato that has evaded classification by solanologists for decades has been identified and is described as a new species belonging to the Australian “Solanum dioicum group” of the Ord Victoria Plain biogeographic region in the monsoon tropics of the Northern Territory. Although now recognised to be andromonoecious, S. plastisexum Martine & McDonnell, sp. nov. exhibits multiple reproductive phenotypes, with solitary perfect flowers, a few staminate flowers or with cymes composed of a basal hermaphrodite and an extended rachis of several to many staminate flowers. When in fruit, the distal rachis may abcise and drop. A member of …

Measuring Flowering Phenology And Its Consequences: A Systematic Review, Samantha M. Mangum

USF Tampa Graduate Theses and Dissertations

Despite growing interest in flowering phenology among ecologists, as reflected by an increase in the number of papers, there is little information on how studies typically measure and describe a plant’s flowering phenology. The focus of this study was the literature on flowering phenology and the approaches researchers have taken to quantify flowering phenology. Initially, a comprehensive description of the breath of literature on flowering phenology was produced. From there, I described the current research on flowering phenology: the year that the studies were published, the locations of the studies, and the particular biomes where the studies were performed. The …

An Evaluation Of The Medicinal Plants Of Andros Island, Bahamas, Vivian Pedrozo

Theses and Dissertations

The earliest civilizations have been using plants as their foundation for healing and medicinal traditions for thousands of years. The use of plant medicinals, more commonly known as “bush medicine” on Andros Island, has been rapidly decreasing due an increased presence of western medicine and increased population pressures from urbanization that are threatening local biodiversity hotspots. Ethnobotany is not only important from a conservation standpoint but is also used to preserve traditional knowledge of herbal medicinals and applications, the people of Andros continue to rely on bush medicine, mainly in the form of teas to help manage health related problems. …

Isoprene Emission In Polytrichaceae Mosses, Timea Deakova

Dissertations and Theses

Our first aim was to identify and quantify Biological Volatile Organic Compound (BVOC) emissions, specifically emissions of isoprene, from the moss Polytrichum juniperinum during its earliest stage of life. Isoprene emission from mosses could be a significant component of the total global budget of BVOC emissions. Data concerning the spatial and temporal variability of these emissions are lacking due to poor characterization of the physical and biological factors controlling isoprene synthesis in both vascular and non-vascular plants. We found that P. juniperinum in its early life stage (protonema) can emit isoprene at detectable levels at day five after spore germination. …

Mapping A New Disease Resistance Locus In An F1 Progeny Derived From Two Grape Wild Relatives, Gaurab Bhattarai

Graduate Theses/Dissertations

Linkage maps and quantitative trait loci (QTL) analysis have become essential tools for the positional cloning of agronomically important genes and for marker-assisted breeding. In this study, two North American grape species, Vitis rupestris and Vitis riparia, and their 294 F₁ progeny were used to construct parental linkage maps and to perform QTL analysis for downy mildew resistance. Single nucleotide polymorphism (SNP) discovery was accomplished using genotyping-by-sequencing (GBS) and resulted in 348,888 SNPs. Of these, 11,063 informative SNP markers (3.17% of the original SNP dataset) were derived after filtering for various quality parameters and missing data. A two-way …

Warming Up: Climate Change Related Shifts Of Mycorrhizal Fungal Communities In High Latitude Ecosystems, Megan Rae Devan

Biology ETDs

This dissertation examines how climate change affects mycorrhizal fungal communities in boreal and arctic ecosystems. In chapter one, I revealed that increases in fire severity and related increases in deciduous tree dominance result in greater Ascomycota relative abundance (RA) and subsequent declines in Basidiomycota RA. In chapter two I analyzed the effects of post-fire mycorrhizal fungal communites on host growth. There were trends at the fungal genus level that were largely reflected at the guild level across all hosts; however, there were some fungal genera that had the opposite effect on different host species. In chapter three, I found host …

A Gradient Of Pollination Specialization In Three Species Of Bolivian Centropogon, Laura Lagomarsino, Nathan Muchhala

Biology Department Faculty Works

PremiseClosely related plant species with overlapping ranges often experience competition for pollination services. Such competition can select for divergence in floral traits that attract pollinators or determine pollen placement. While most species in Centropogon(Campanulaceae: Lobelioideae) have flowers that suggest adaptation to bat or hummingbird pollination, actual pollinators are rarely documented, and a few species have a mix of traits from both pollination syndromes. We studied the pollination biology of a “mixed‐syndrome” species and its co‐occurring congeners to examine the relationship between floral traits and visitation patterns for Centropogon.Methods Fieldwork at two sites in Bolivian cloud forests involved filming floral visitors, …

Fusarium Euwallacea: A Serious Threat To The Native And Ornamental Trees And Shrubs In Southern California, Greg Tyler, Yixing Zheng, Michael Kulinich, Hagop Atamian

Student Scholar Symposium Abstracts and Posters

Fusarium Euwallacea is a fungus that has established symbiotic relationship with the beetle Euwallacea aff. fornicata. The beetle bores through the tree bark and into the sapwood making long tunnels inside the trees. The beetle carries the F. Euwallacea in a specialized structure on its body called mandibular mycangia and cultivates the fungus in the tunnels on which the beetle feeds to grow and reproduce. The growth of the fungus obstructs water and mineral transport in the plant xylem tissue, resulting in dieback, wilt and mortality of the host tree. Fungi are known to secrete proteins called effectors in …

Tree To Grass Water Use Ratios; Assessing Turfgrass’ High Water Use In The Urban Landscape, Tamara Wynne

UNLV Theses, Dissertations, Professional Papers, and Capstones

Water demand in the southwestern United States continues to rise. The population of the Las Vegas Valley doubled from 2000-2010 and now more than two million people call it home. The residential sector uses 60% of all water consumed in the valley. Outdoor urban landscape irrigation is responsible for 70% of all residential use. These landscapes are dominated by trees and turf grass. Although the water use of turf grass species is well studied, there are few published results about the water use of landscape trees in the desert southwest USA. To obtain a more complete picture of the tradeoffs …

Species Richness Patterns And Plant Size Of Vascular Epiphytes Along An Elevation Gradient In The Tropical Montane Forest Of Volcán Maderas, Nicaragua., Hazel Krupzkaya Berrios Alvarez

Student Theses and Dissertations

Aim To quantify distribution patterns of vascular epiphytes and determine how climate gradients drive species turnover patterns of tropical montane cloud forest systems. Location Volcán Maderas, Isla de Ometepe, Nicaragua. Methods Surveys were conducted in five distinct forest types as follows: Dry Forest, Humid Forest, Wet Forest, Cloud Forest, and Elfin Forest. Results A hump-shaped distribution pattern was detected for all vascular epiphytes at approx.1000 m in elevation (Cloud Forest). Overall species turnover was high for vascular epiphytes along the elevation gradient and was mainly driven by water availability. In addition, changes in size of vascular epiphytes were detected. Main …

Trans-Cinnamic Acid-Induced Leaf Expansion Involves An Auxin-Independent Component, Jasmina Kurepa, Jan A. Smalle

Plant and Soil Sciences Faculty Publications

The phenylpropanoid pathway, the source of a large array of compounds with diverse functions, starts with the synthesis of trans-cinnamic acid (t-CA) that is converted by cinnamate-4-hydroxylase (C4H) into p-coumaric acid. We have recently shown that in Arabidopsis, exogenous t-CA promotes leaf growth by increasing cell expansion and that this response requires auxin signaling. We have also shown that cell expansion is increased in C4H loss-of-function mutants. Here we provide further evidence that leaf growth is enhanced by either t-CA or a t-CA derivative that accumulates upstream of C4H. We also show that …

Phylogeny Of The Australian Solanum Dioicum Group Using Seven Nuclear Genes: Testing Symon’S Fruit And Seed Dispersal Hypotheses., Christopher T. Martine, Ingrid E. Jordon-Thaden, Angela J. Mcdonnell, Jason T. Cantley, Daniel S. Hayes, Morgan D. Roche, Emma S. Frawley, Ian S. Gilman, David C. Tank

Faculty Journal Articles

The dioecious and andromonoecious Solanum taxa (the “S. dioicum group”) of the Australian Monsoon Tropics have been the subject of phylogenetic and taxonomic study for decades, yet much of their basic biology is still unknown. This is especially true for plant-animal interactions, including the influence of fruit form and calyx morphology on seed dispersal. We combine field/greenhouse observations and specimen-based study with phylogenetic analysis of seven nuclear regions obtained via a microfluidic PCR-based enrichment strategy and high-throughput sequencing, and present the first species-tree hypothesis for the S. dioicum group. Our results suggest that epizoochorous trample burr seed …

Post-Fire Competition Between Native Ceanothus Spinosus And Exotic Grasses, Katarina Carter, Nina Duchild

Featured Research

In our experiment, we investigated the post-fire competition between native Ceanothus spinosus and exotic grasses in low and high-density fuel sites. We hypothesized that C. spinosus seedlings in the high-density fuel site would be larger and more numerous than seedlings in the low-density fuel site, due to differences in competition from exotics. Our results showed that there was no significant difference in seedling height of C. spinosus in the high-density fuel site (5.33 ± 0.55 cm) and in the low-density fuel site (4.31 ± 0.51 cm). There was also no significant difference in the average number of seedlings in the …

Physiological Performance Of Ceanothus Spinosus In Hot Burn And Cool Burn Sites, Rishi Gattu, Christian Hill, Chris Kulpaca

Featured Research

After the Woolsey Fire, many fire adapted plants will resprout. However, increased fire frequency has negatively impacted the reproduction of many native species, while enhancing the survivorship of many invasive species. We compared the physiological performance of Ceanothus spinosus resprouters in areas that burned hotter compared to areas that burned cooler. We hypothesized that resprouters in hotter burn sites would have better physiological performance than resprouters in cooler burn sites. The resprouters in hotter burn sites would have better performance because of decreased competition from invasive species. We measured physiological performance using a Li-Cor LI-6400, specifically photosynthesis, transpiration, and stomatal …