Biology Commons^™

Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott

Faculty Works

The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …

The Potential Role Of Phenotypic Plasticity In The Ability Of Hydrocotyle Bonariensis To Occupy Two Different Habitats, Harold Ralph Parsons Iii

Honors College Theses

Phenotypic plasticity is the ability of an organism to change morphology and/or physiology in response to changes in the environment. Hydrocotyle bonariensis is a coastal perennial herb found in both coastal sand dunes and inland coastal plain habitats in Georgia. The purpose of this study was to determine if there were differences in leaf morphology for populations of H. bonariensis in coastal sand dune and inland coastal plain habitats. Leaf morphology and microenvironmental variables were compared between H. bonariensis populations at Tybee Island (TI), GA, representing the coastal sand dune habitat, and Georgia Southern University Armstrong Campus (GSU) in Savannah, …

Factors Influencing Tree Growth In A Bottomland Hardwood Along The Green River In Kentucky, Caleb Miller

Masters Theses & Specialist Projects

In bottomland hardwood forests, large fluctuations in moisture availability can lead to stress from both flooded and drought conditions. These forests can have a complex species composition with different water use and drought strategies. The bottomlands in which these species co-occur can have a complex spatial distribution of alluvial sediments due to the meandering and migration of river or stream channels, which may impact the hydrology, nutrient dynamics, and productivity. At Western Kentucky University's Green River Preserve, approximately 9 ha of bottomland hardwood forest were planted in 2006. Although these hardwoods have received equal amounts of sunlight, rainfall, and wind, …

Investigation Into The Responses Of Physcomitrella Patens To Herbivory And The Influence Of Light On The Production Of Defense Compounds, Matthew Morris

Undergraduate Honors Thesis Collection

Plant defense mechanisms are well studied in many agriculturally relevant crops such as corn and tomatoes. Though less studied, the more ancestral nonvascular plants may be able to provide insights into the origin and working of modern plant defense systems. In this study, the bryophyte Physcomitrella patens was researched and the role of the jasmonic acid pathway in response to herbivory was investigated. Additionally, the impact of light intensity on the efficacy of this pathway was to be determined. After exposing the moss to various chemical elicitors and mechanical wounding, the activity of proteins produced in the JA pathway was …

Agricultural Practices And Nitrogen Cycling Microbes: The Impact Of Sustainable Farming Practices On Nutrient Movement In Indianapolis Urban Farms, Margaret Davis

Undergraduate Honors Thesis Collection

As population movement to urban centers and decreased availability of fresh foods becomes more common, the prevalence of food deserts is becoming far greater. Urban farming can potentially help address these issues by bringing healthy and fresh food sources directly to these areas that lack access to quality food. Urban farming is reliant on the implementation of sustainable practices, like the use of cover crops, to increase the amount of nutrients that are accessible to plants from the soil. Nitrogen is a vital nutrient but cannot be readily produced by plants, so it must be obtained by either an external …

Examining The Invasion Of Bush Honeysuckle Through A Phylogenetic Analysis, Phoebe Bruffett

Biological Sciences Undergraduate Honors Theses

The mechanisms underlying the continual spread of invasive plants within their respective non-native ranges is a major focal point to invasion research. Many theories have been proposed to understand these invasions, each with different implications for the predicted range for invasive plants. Lonicera maackii provides an exceptional opportunity to examine the effectiveness of these theories to explain the trends of invasive plants. Lonicera maackii is native to eastern Asia, but has invaded much of the eastern United States, presenting a severe threat to the health of forest and other natural areas. Recent analysis of the climatic envelopes uncovered a significant …

Through The Leaves: Understanding Population Genetic Structure Of Clematis Morefieldii, Keith Andrew Greenway

Honors Capstone Projects and Theses

No abstract provided.

Epidemiology And Impacts Of A Leaf Spot Disease In Veratrum Viride (Melanthiaceae), Leeah R. Sutton, Foster Levy

Undergraduate Honors Theses

Fungal phytopathogens can cause disease epidemics in crops, weeds, and
populations of native plants. To investigate the impact of a foliar phytopathogen on the native herbaceous species, Veratrum viride, a demographic and disease assessment was carried out on two high elevation grassy bald populations on Roan Mountain, Tennessee. A leaf spot disease impacted all plants in both populations, causing widespread premature senescence of leaves and stems. Disease severity increased over the course of the growing season. Based on host disease symptoms and fungal conidia morphology, Pseudocercosporella sublineolata was shown to be the causal pathogen. A study of herbarium specimens …

Winners And Losers In Dryland Reforestation: Species Survival, Growth, And Recruitment Along A 33-Year Planting Chronosequence, Clifton Albrecht, Zarek Contreras, Kimberly Wahl-Villarreal, Mitch Sternberg, Bradley O. Christoffersen

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Reforestation in the Lower Rio Grande Valley (LRGV) of Texas began in the 1960s and to date over 6,475 ha of land has been reforested. However, there has been minimal assessment to understand differential species success, compositional trends, and the aboveground C sequestration potential of these developing forests. We coupled quantitative planting information of >50 native woody tree and shrub species with surveys of 5,223 stems of 4,606 individuals in a chronosequence of restored forests ranging in age from 1 to 33 years to estimate species-specific mortality rates, biomass accumulation and recruitment, as well as compositional trends in the herbaceous …

Towards Conserving Crop Wild Relatives Along The Texas–Mexico Border: The Case Of Manihot Walkerae, Gisel Garza, Crystian Sadel Venegas Barrera, Jon Dale, José Guadalupe Martínez-Ávalos, Teresa Patricia Feria-Arroyo

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Walker’s Manihot, Manihot walkerae, is an endangered species endemic to south Texas and northeastern Mexico and is a Crop Wild Relative (CWR) of the international and economically important crop cassava (M. esculenta). Manihot walkerae is globally endangered (IUCN’s Redlist, Texas list, USA); however, it is not recognized on the Mexican list of endangered species (NOM-059-SEMARNAT). We assessed the status of M. walkerae in Mexico and re-evaluated its global status. According to our analysis, M. walkerae should be considered an endangered species based on the IUCN’s assessment method and a threatened species in Mexico based on the Mexican criteria. Our findings …

Microbial Diversity And Community Structure In Sediments Associated With The Seagrass (Thallassia Testudinum) In Apalachicola Bay, Florida, Rahma Ahmed, Thomas Mcelroy, Troy Mutchler

Symposium of Student Scholars

Seagrass is an angiosperm which provides many ecosystem services in coastal areas, such as providing food, shelter and nurseries for many species, and decreasing the impact of waves on shorelines. A global assessment reported that 29% of known seagrass meadows are in a state of decline due to the effects of human activity. Seagrass is commonly found in shallow marine waters where they form meadows containing a microbiome that plays an important role in providing nutrients for seagrass growth, though little is known about the microorganisms within the seagrass meadow sediments. Our project collected sediments from seagrass meadows and adjacent …

The Effect Of Clay On The Inhibitory Effect Of Mustard Seed Meal On Velvetleaf Seedling Growth, Heeyun Oh

Honors Theses

Mustard seed meal (MSM) has been studied as a biofumigant in suppressing weed growth in an organic-friendly way. Previous studies have shown that MSM in the presence of elevated sand content was highly effective in suppressing the germination of velvetleaf seeds, and that as clay/silt in sand content increased, MSM effectiveness decreased in suppressing seed germination. Based on these results, it was postulated that clay may have effects on MSM by inhibiting this biofumigant from suppressing weed growth. Two different types of pure clay--kaolin and montmorillonite--were selected with their increasing concentrations. Low concentrations of clay do not seem to influence …

The Plant And The Pathogen: Elucidating The Relationship Between Root-Knot Nematodes, Ph Levels, And Arabidopsis Thaliana Development, Rachel Garnett

Student Scholar Showcase

Root-knot nematodes (RKNs) within the Meloidogyne genus are considered one of the largest threats to plant health and subsequent crop yield and profit (Forghani and Hajihassani 2020; Bernard et al. 2017). As a pest that presents global consequences, its mitigation through sustainable interventions may confer results for the treatment of similar plant pathogens. A member of the Brassicaeae family, Arabidopsis thaliana, was chosen for this study to determine plant responses to RKN-standard pH media. pH was varied between control and experimental groups, and phenotypic variables of primary root length and plant height were observed and analyzed. Wild-type Arabidopsis thaliana …

Leaf Phenology Of Callery Pear: Frost Damage, Margaret E. Maloney, Abby Hay, Eric B. Borth, Ryan W. Mcewan

Five Rivers MetroParks Collaboration Data Archive

This dataset is one of three affiliated with the paper "Leaf phenology and freeze tolerance of the invasive tree Pyrus calleryana Roseaceae) and potential native competitors."

The other two datasets are:

• Leaf Phenology of Callery Pear: Spring
• Leaf Phenology of Callery Pear: Fall

Leaf Phenology Of Callery Pear: Spring, Margaret E. Maloney, Abby Hay, Eric B. Borth, Ryan W. Mcewan

Five Rivers MetroParks Collaboration Data Archive

This dataset is one of three affiliated with the paper "Leaf phenology and freeze tolerance of the invasive tree Pyrus calleryana Roseaceae) and potential native competitors."

The other two datasets are:

• Leaf Phenology of Callery Pear: Fall
• Leaf Phenology of Callery Pear: Frost Damage

Cryptic Hybridization In The Temperate Bamboos: Is Pleioblastus Simonii A Species Of Hybrid Origin?, Morgan Brown

Theses

Japanese river bamboo (Pleioblastus simonii, ‘medake,’‘kawadake’) is an ecologically important species of temperate bamboo native to Japan. This species is widely known and historically important in Japanese rural farm life. Based on morphological data, Japanese river bamboo is classified in Pleioblastus section Medakea (Poaceae: Bambusoideae) along with five other Japanese species, which are collectively considered to represent a phylogenetically distinct lineage. However, recent studies suggest that Japanese river bamboo may have arisen as a result of previously undetected hybridization (i.e., cryptic hybridization), while also calling into question the diversity of section Medakea. The role of hybridization in natural plant populations …

Life Line Spring 2022, Otterbein Biology And Earth Science Department

Life Line - The Biology Department Newsletter

In this Issue:

A CURE for the Typical STEM Classroom; Attracting Cardinals; Svitana Provides Training to IHS; The Cardinal Science Scholar Program; Mornings with Manatees; Viewing the Department from a New Perspective; Science Lecture Series Features Dr. Tyrone Hayes; Internship with Beinhower Brothers Drilling Company; New Student Chapter: The Botanical Society of America; STEM Art Show 2021; Got Bones?; The Robert and Ann Harting Endowed Award; Grossology Returns; Class Flocks to National Aviary

Leaf Phenology Of Callery Pear: Fall, Margaret E. Maloney, Abby Hay, Eric B. Borth, Ryan W. Mcewan

Five Rivers MetroParks Collaboration Data Archive

This dataset is one of three affiliated with the paper "Leaf phenology and freeze tolerance of the invasive tree Pyrus calleryana Roseaceae) and potential native competitors."

The other two datasets are:

• Leaf Phenology of Callery Pear: Spring
• Leaf Phenology of Callery Pear: Frost Damage

Screening Commercial Entomopathogenic Fungi For The Management Of Diaphorina Citri Populations In The Lower Rio Grande Valley, Texas, Usa, Justin Wendel, Jonathan Cisneros, Stefan Jaronski, Christopher Vitek, Matthew Ciomperlik, Daniel Flores

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Ten strains of entomopathogenic ascomycete fungi, sourced from commercial formulations of blastopore or conidiospore formulations, were tested in 14 different formulations in a primary acquisition/direct spray bioassay against adult Asian citrus psyllid (Diaphorina citri Kuwayama (Hemiptera: Liviidae)). The Cordyceps (Isaria) javanica Apopka 97-C (conidia) strain was used as the standard. A statistical ranking system was established in which top performing pathogenic strains were selected for further screening and eventual field trials. Modified Potter-type spray towers were utilized to deliver a range of doses of viable spores to adult D. citri in an aqueous spray consistent with …

A Comparison Of Natural, Living, And Hardened Shorelines Ability To Prevent Coastal Erosion And Maintain A Healthy Ecosystem, Gabrielle Spellmann

Master's Theses

It is important to find a suitable method to protect the U.S. Gulf Coast shoreline, since its’ low elevation and the Loop current make it vulnerable to sea level rise. I focused on two manmade methods, hardened, and living shorelines, of coastal protection for when the natural marsh suffers excess erosion rates. Living shorelines are a suite of shoreline conservation and restoration techniques that usually involve some sort of hardened structure that dampens wave energy so that the native vegetation behind it can take root and stabilize the shoreline. This study looked at six different sites, all containing a natural, …

Source Of Off-Type Frequencies In Winter Wheat Varieties, Michaela Benes

Honors Program: Senior Projects (Public)

The enzyme polyphenol oxidase (PPO) is undesirable in most wheat products due to the discoloration it causes. Purifying wheat varieties through selective breeding is the best way at getting rid of PPO in a variety. The variety NW15443 was planted in both a field in McCook, NE, and in a greenhouse in Lincoln, NE. Individual heads were snapped from the field and the greenhouse seed was harvested in bulk. After completing a PPO test of the seed, it was determined that the percentages of off-type frequencies from the field seed and the greenhouse seed were 11% and 3.6%, respectively. Using …

Salicylic Acid And N-Hydroxypipecolic Acid At The Fulcrum Of The Plant Immunity-Growth Equilibrium, Alyssa Shields, Vanessa Shivnauth, Christian Danve M. Castroverde

Biology Faculty Publications

Salicylic acid (SA) and N-hydroxypipecolic acid (NHP) are two central plant immune signals involved in both resistance at local sites of pathogen infection (basal resistance) and at distal uninfected sites after primary infection (systemic acquired resistance). Major discoveries and advances have led to deeper understanding of their biosynthesis and signaling during plant defense responses. In addition to their well-defined roles in immunity, recent research is emerging on their direct mechanistic impacts on plant growth and development. In this review, we will first provide an overview of how SA and NHP regulate local and systemic immune responses in plants. We …

Comparison Of Tardigrade Extraction Methods, Abigail Andros

Library/Life Sciences Undergraduate Poster Competition 2022

It is a time-consuming process to pick tardigrades for an experiment. The standard method is to add water to the sample, then look through it and pick out tardigrades with a micropipette or small wire loop (Tsujimoto et al. 2015).

Overcoming Detrimental Potato Fungi With The Secondary Metabolites Of Naturally Occurring Streptomyces In A Greenhouse, Hansan Jones, Jacob Anderson, Erik Kemp, Brad Geary, Matthew Madsen, Ryan Stewart

Library/Life Sciences Undergraduate Poster Competition 2022

• Fungal disease Silver scurf can cause 13% water loss and give poor aesthetic appeal, both of which reduce farmers profits (1,2).
• Pythium leak can result in up to 50% total loss of potatoes in storage (3).
• Streptomyces is the right candidate to mitigate or eliminate these losses because of its ability to survive in soil (4) and its history as a proven biocontrol for other plant pathogens (5).

Data From Callery Pear Prairie Survey, Michaela J. Woods, Grace Dietsch, Ryan W. Mcewan

Five Rivers MetroParks Collaboration Data Archive

This dataset was curated following the examination of two prairies invaded by Callery pear, and two that were not invaded. We assessed soil nutrients and chemistry, plant cover, and proximity to different land uses to see if they impacted Callery pear abundance, presence/absence or cover.

Taxonomic And Phylogenetic Diversity Of Pitcher Plant Bogs In Georgia's Coastal Plain, Melanie Flood

Theses and Dissertations

Diversity studies via floristic survey and taxonomic metrics have proven essential for discerning community composition and biodiversity, however, phylogenetic analyses are necessary to reveal underlying evolutionary change and potential community assembly patterns. Pitcher plant bogs are highly diverse, highly understudied habitats that contain numerous listed and at-risk plant and animal species of Georgia.

In this study I investigated the taxonomic and phylogenetic diversity of pitcher plant bogs by asking the following questions:

1) What is the taxonomic diversity of pitcher plant bogs,

2) What is the dispersion of phylogenetic diversity within bog habitats, and

3) Does taxonomic and phylogenetic diversity …

Phylogenomic Discordance Suggests Polytomies Along The Backbone Of The Large Genus Solanum, Edeline Gagnon, Rebeccca Hilgenhof, Andrés Orejuela, Angela J. Mcdonnell, Gaurav Sablok, Xavier Aubriot, Leandro Giacomin, Yuri Gouvêa, Thamyris Bragionis, João Renato Stehmann, Lynn Bohs, Steven Dodsworth, Christopher T. Martine, Péter Poczai, Sandra Knapp, Tiina Särkinen

Faculty Journal Articles

Premise of the study

Evolutionary studies require solid phylogenetic frameworks, but increased volumes of phylogenomic data have revealed incongruent topologies among gene trees in many organisms both between and within genomes. Some of these incongruences indicate polytomies that may remain impossible to resolve. Here we investigate the degree of gene-tree discordance in Solanum, one of the largest flowering plant genera that includes the cultivated potato, tomato, and eggplant, as well as 24 minor crop plants.

Methods

A densely sampled species-level phylogeny of Solanum is built using unpublished and publicly available Sanger sequences comprising 60% of all accepted species (742 spp.) …

Assessment Of Two Novel Host-Derived Beauveria Bassiana (Hypocreales: Cordycipitaceae) Isolates Against The Citrus Pest, Diaphorina Citri (Hemiptera: Liviidae), Jonathan Cisneros, Justin Wendel, Stefan Jaronski, Christopher Vitek, Matthew Ciomperlik, Daniel Flores

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), vectors ‘Candidatus Liberibacter spp.’, the causative agent of Citrus Greening Disease (CGD) or Huanglongbing (HLB). Managing populations of psyllids in the Lower Rio Grande Valley (LRGV), TX, United States is imperative given a continuous increase in HLB-positive trees. A component of integrated pest management (IPM) program is the use of strains of entomopathogenic fungi for the biological control of D. citri. In an attempt to find endemic strains of entomopathogenic fungi that grow favorably under LRGV environmental conditions and naturally infect D. citri, psyllids were collected from …

Review Of The Invasive Forage Grass, Guinea Grass (Megathyrsus Maximus): Ecology And Potential Impacts In Arid And Semi-Arid Regions, Pushpa Soti, Vanessa Thomas

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Several introduced African grasses are known to present recurring patterns of invasiveness and cause a severe impact on the diversity and functioning of ecosystems worldwide. Megathyrsus maximus (Guinea grass), a forage grass species native to South Africa, is reported to be highly invasive and pose a serious threat to native biodiversity in the introduced range. Despite the severe ecological threats posed by M. maximus worldwide, there is a dearth of information on the ecological and agroecological impact of M. maximus when growing in unintended areas. In this review, we present general information on M. maximus, its distribution and ecological …

Plant Canopy May Promote Seed Dispersal By Wind, Xuanping Qin, Wei Liang, Zhimin Liu, Minghu Liu, Carol C. Baskin, Jerry M. Baskin, Zhiming Xin, Zhigang Wang, Quanlai Zhou

Biology Faculty Publications

Seed dispersal has received much research attention. The plant canopy can intercept diaspores, but the effect of the plant canopy (the aboveground portion of a plant consisting of branches and leaves) on dispersal distance has not been explored empirically. To determine the effect of plant canopy on seed dispersal distance, a comparison of diaspores falling through open air and through plant canopy was made in a wind tunnel using three wind speeds and diaspores with various traits. Compared with diaspores falling through open air, the dispersal distance of diaspores falling through plant canopy was decreased or increased, depending on wind …