Biology Commons^™

The Impact Of Soil Disturbance On Soil Bacterial Community Composition, Marie A. Rodriguez, Mark Peach, Timothy D. Trott

Research in Biology

Soil bacterial communities are an important part of terrestrial ecosystems due to their roles in biogeochemical cycling processes. Consequently, understanding how soil disturbance affects the soil bacterial diversity is vital to understanding the entire ecosystem. In this study we examined the effects of soil disturbance (by mining) on the soil bacterial community composition from three sites on Bauxite Ridge in Southeast Tennessee compared to three undisturbed sites in a nearby location. The soil bacterial community was analyzed by 16S rDNA amplicon sequencing of total DNA extracted from the soil samples collected from each of the six sites. Characterization of the …

Evidence Of Competitive Release Following Overstory Mortality In A Semi-Arid Piñon-Juniper Woodland, Corrie D. Gonzalez

Biology ETDs

Extreme temperatures and severe drought events have led to widespread tree mortality worldwide. In semi-arid regions of the Southwest United States, these events pose a significant threat to piñon-juniper (PJ) woodlands. We studied the effects of piñon and juniper mortality on the growth and physiology of existing saplings in PJ woodlands by analyzing water status, photosynthetic activity, and tissue chemistry to gain insights into these impacts. Juniper saplings exhibited improved water status and water use efficiency in response to overstory mortality, whereas piñon saplings did not. Additionally, both piñon and juniper saplings exhibited increased photosynthetic rates, increased photosynthetic capacity, and …

Structural Diversity And Stress Regulation Of The Plant Immunity-Associated Calmodulin-Binding Protein 60 (Cbp60) Family Of Transcription Factors In Solanum Lycopersicum (Tomato), Vanessa Shivnauth, Sonya Pretheepkumar, Eric J. R. Marchetta, Christina A. M. Rossi, Keaun Amani, Christian Castroverde

Biology Faculty Publications

Cellular signaling generates calcium (Ca2+) ions, which are ubiquitous secondary messengers decoded by calcium-dependent protein kinases, calcineurins, calreticulin, calmodulins (CAMs), and CAM-binding proteins. Previous studies in the model plant Arabidopsis thaliana have shown the critical roles of the CAM-BINDING PROTEIN 60 (CBP60) protein family in plant growth, stress responses, and immunity. Certain CBP60 factors can regulate plant immune responses, like pattern-triggered immunity, effector-triggered immunity, and synthesis of major plant immune-activating metabolites salicylic acid (SA) and N-hydroxypipecolic acid (NHP). Although homologous CBP60 sequences have been identified in the plant kingdom, their function and regulation in most species remain unclear. In …

Morphological Trait Evolution In Solanum (Solanaceae): Evolutionary Lability Of Key Taxonomic Characters, Rebecca Hilgenhof, Edeline Gagnon, Sandra Knapp, Xavier Aubriot, Eric Tepe, Lynn Bohs, Leandro Giacomin, Yuri Gouvea, Andres Orejuela, Christopher T. Martine, Clara Ines Orozco, Iris E. Peralta, Tina Sarkinen

Faculty Journal Articles

Solanum is one of the world's largest and economically most important plant genera, including 1245 currently accepted species and several major and minor crops (e.g., tomato, potato, brinjal eggplant, scarlet eggplant, Gboma eggplant, lulo, and pepino). Here we provide an overview of the evolution of 25 key morphological traits for the major and minor clades of this giant genus based on stochastic mapping using a well-sampled recently published phylogeny of Solanum. The most evolutionarily labile traits (showing >100 transitions across the genus) relate to plant structure (growth form and sympodial unit structure), herbivore defence (glandular trichomes), pollination (corolla shape …

Soil Moisture Dynamics In Cover Cropping Systems: From Local To Global Scales, Manish Gautam

Theses and Dissertations

Cover cropping systems, which have gained recognition for their ability to enhance soil health and promote sustainability are also associated with potential risks related with declining soil moisture and pose a dilemma for farmers considering the adoption of cover crops in water deficit semi-arid settings worldwide. To address this pressing issue, we conducted a participatory cover cropping trial in the Lower Rio Grande Valley (LRGV) region, encompassing four different farms and supplemented this experiment with a meta-analysis to answer our soil moisture and climate parameters and quantify the impact of cover crops on soil moisture levels. The findings revealed that …

Comparative Analysis Of Soil Properties, Microbial Community Composition, And Yield Responses To Cover Crops In Different Ecoregions Of South Texas, Daphne Zapsas

Theses and Dissertations

Comprehensive understanding of soil properties, their interdependencies, and the effects of management practices is crucial for designing sustainable agricultural strategies. By adopting site-specific and need-based practices that enhance soil health, farmers can optimize crop growth, increase yield, and contribute to long-term environmental sustainability. Cover crops have been recognized for enhancing soil fertility, promoting microbial activity, and increasing organic matter content. However, while their effectiveness is well documented in temperate regions with readily available water, their impact in water limited systems in subtropical regions remains mixed. The aim of this study is to assess soil conditions in four different farms across …

Cadmium And Salinity Stressor Antagonism On Vallisneria Neotropicalis, Christopher P. Mikolaitis

Graduate Theses and Dissertations (2019 - present)

Submerged macrophytes form the foundation of freshwater ecosystems. These organisms are sessile and are very susceptible to shifts in their environment. Heavy metals are of particular concern as they can be sequestered indefinitely in sediments and are readily taken up by rooted vegetation. In the presence of saltwater intrusions, these metals can interact with salt ions potentially changing their availability to submerged vegetation. In this study a freshwater macrophyte, Vallisneria neotropicalis, was used as a test species for interactive effects between Cd, a non-essential heavy metal, and salt stress. The metrics used to establish the individual as well as …

Efficacy Of The Botanical Extracts, Azadirachta Indica (Sapindales: Meliaceae) And Tagetes Minuta (Asterales: Asteraceae) In The Control Of Cabbage Insect Pests In Iringa District, Tanzania, Levocatus M. Rwegoshora, Vendeline E. Tairo, Moses I. Olotu

Tanzania Journal of Science

Although botanical extracts have been widely studied globally, the efficacy of neem and Mexican marigold against cabbage insect pests is scanty. Field experiments were conducted at Kalenga and Mgera in 2022 using a randomized complete block design. Four treatments (neem, Mexican marigold, a mixture of the two extracts and untreated) were replicated three times. In Kalenga, damage levels for treated plots varied significantly from 3.33 to 9.20% and 5.0 to 18.33%, while for untreated plots varied from 37.70 to 45.85% during the rainy and dry seasons, respectively (F(3, 499) = 111.71, p < 0.05). A similar trend was recorded in Mgera, the damage levels varied significantly between treated (4.44–15.83%) and untreated plots (34.40–46.60%) (F(3, 449) = 94.23, p < 0.05). Treated plots had higher marketable cabbage yields (30.6–43.10 t/ha) than untreated plots (4.78–11.20 t/ha), which differed significantly in Kalenga (F (3, 67) = 141.79, p < 0.05) and Mgera (F (3, 67) = 53.36, p < 0.05). These extracts have shown insecticidal properties, can serve as promising candidates for further studies aimed at isolating active compounds for scaling up ecologically friendly strategies of controlling pests and improve the quality of cabbage products.

In-Vitro Antisickling Activity Of Pergularia Daemia, Canna Indica And Petiveria Alliacea Plants Used In The Treatment Of Sickle Cell Anaemia In Edo State, Nigeria, Osaro Iyekowa, Felix E Okieimen, Clifford O Ehisuoria

Tanzania Journal of Science

Sickle cell disease is a genetic blood disorder that affects the shape and transportation of the red blood cells (RBCs) in blood vessels, leading to various clinical complications. Available medicines for treating the disease are insufficiently effective, toxic or expensive. Therefore, there has been a pressing need for effective and inexpensive therapeutic agents from indigenous medicinal plants. Petiveria alliacea, Canna indica and Pergularia daemia, respectively were evaluated for their cationic constituents as a measure for their antisickling activity in sickle cell anaemia disorder. The three medicinal plants were extracted separately with methanol solvent using maceration method for 72 hours. The …

Acarosporaceae Of New Mexico: Eight New Species Of Acarospora And Sarcogyne, Kerry Knudsen, Jana Kocourková, Eva Hodková, Jiří Malíček, Yan Wang

Western North American Naturalist

Eight new species are described from the Chihuahuan Desert in southern New Mexico: Acarospora agos­tiniana, A. divisa, A. fissurata, A. ryanii, Sarcogyne brouardiana, S. coeruleonigricans, S. malpaiensis, and S. nogalensis. Three species, Acarospora coloradiana, A. peltastica, and A. utahensis are not treated as synonyms of A. strigata, a South American endemic. Thirty-four species of Acarosporaceae are reported from the Chihuahuan Desert in southern New Mexico. Fifty-six species of Acarosporaceae are reported from New Mexico. It is estimated that 416 species of described Acarosporales occur worldwide, most in need of revisions. …

A Remote Sensing Approach To Assess The Historical Invasion Of Phragmites Australis In A Brackish Coastal Marsh, Jason S. Hagani, John Y. Takekawa, Steven C. Chappell, Richelle L. Tanner, Adrienne R. Ernst, Karin M. Kettenring

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Introduction: Coastal estuarine wetlands provide important habitats for a variety of endemic flora and fauna but are particularly vulnerable to biological invasions. Regular monitoring of changes in these vulnerable wetlands has become increasingly important for effective management, especially considering threats from climate change effects and human disturbance. Historical analyzes of plant invasions may guide targeted management strategies to eradicate harmful species. Estimating the distribution of invasive species has never been more accessible with the improved availability of high-resolution data and innovations in remote sensing, estimating the distribution of invasive species has never been more accessible.

Methods: We assessed the spread …

Measuring The Physiological Effects Of Methylobacterium Oryzae Foliar Inoculation Onto Salt-Stressed Tomatoes Grown In A Greenhouse Semi-Hydroponic System, Collin Dice

Master of Science in Integrative Biology Theses

Salinity stress has been shown to improve hydroponic produce quality such as that of tomato but at the trade-off of produce yield. In order to improve demand for hydroponic produce, techniques that improve salinity tolerance could offset declines in yield without altering the improved quality of produce. In two separate trials, we tested whether foliar inoculation of the ACC deaminase-producing bacteria Methylobacterium oryzae CBMB20 improved salt tolerance in tomatoes grown in a semi-hydroponic system using an indeterminate variety exposed to 26mM NaCl (Trial 1) and a determinate variety exposed to 17mM NaCl (Trial 2). We also tested how salinity and …

Reintroducing Hemp (Rongony) In The Material Palette Of Madagascar: A Study On The Potential Of Hemp Clay Components And Its Impact On Social And Ecological Communities., Henintsoa Thierry Andrianambinina

Masters Theses

When mentioning the word hemp, especially in the local language of Madagascar, the literal translation does not set it apart from marijuana, as they are both called “rongony” - creating the stigma around hemp as the negative stereotype of marijuana. However, the material has been used by the ancestors of Madagascar, as well as across cultures, in its fibrous form to produce fabrication like textile goods and packaging. During colonization, the prohibition of hemp intensified, and since then, any activity related to either of these plants is prohibited and will end in severe punitive measures. This thesis explores the strengths …

Fluidity And Inconstancy: Australian Bush Tomatoes As An Exemplar Of Non-Normative Sex Expression, Christopher T. Martine

Faculty Journal Articles

Solanum, a genus of ~1500 global species, is one of the more interesting plant groups in which to study reproductive biology and ecology. Overwhelmingly, species in this group express full cosexuality, where individual plants have flowers containing both fully-functioning “male” (staminate) and “female” (carpellate) organs. However, there have been multiple and widespread evolutionary transitions within the genus to non-normative variations on this ancestral condition. Australian bush tomatoes (ca. 40 species) are especially diverse in this regard, with uncommon variation and combinations of unisexuality and cosexuality -- including, most notably, two sexual systems known as dioecy (unisexual male or female …

The Role Of Sinorhizobium Meliloti Type Ivb Pili Protein On Legume Nodule Morphology And Infection, Alexis Chun

Honors Thesis

The symbiotic relationship between legumes and rhizobial soil bacteria leads to the conversion of nitrogen gas to biologically available forms of nitrogen. A critical step in this interaction is bacterial attachment to the host root. A variety of factors are involved in attachment, such as the Type IV pili (Tfp). In pathogens, Tfpb mediates tight adherence to host cells, promoting colonization, however in symbiotic interactions, the role of Tfpb–encoded by pilA genes–is not well understood. To assess the role of Tfpb pili during symbiosis, Melilotus alba plants were inoculated with wild type Sinorhizobium meliloti or ΔpilA deletion mutants; roots were …

Interactive Map Code (R And Rstudio), Peter G. Butterfield

Data Files: White Oaks in Vinton Furnace Experimental Forest

Interactive map code puts data and coordinates into readable table format and creates data visualizations.

In Vitro Screening Of 12 Strains Of Birdsfoot Trefoil (Lotus Corniculatus) Against Haemonchus Contortus, Isabella Caldwell, Elizabeth Kass, Rebecca Brown, Katherine Harper Petersson

Senior Honors Projects

Gastrointestinal nematodes (GIN), primarily Haemonchus contortus, are one of the leading causes of death in sheep and goats. As resistance to commercial dewormers continues to rise, alternative strategies for GIN parasite control are needed. Preliminary studies have demonstrated that secondary plant compounds in birdsfoot trefoil (BFT, Lotus corniculatus) possess antiparasitic activity against GIN. In this study, the antiparasitic effect of twelve strains of BFT on the hatching of H. contortus eggs and motility of larvae was determined. BFT strains were harvested in June of 2020 and hay-dried. H. contortus eggs were recovered from mono-infected Dorset yearlings by running fresh …

Estimating The Functional Consequences Of Intra-Specific Trichome Variation Of Tomato, Solanum Lycopersicum On The Specialist Herbivore, Manduca Sexta, Satinderpal Kaur

Theses and Dissertations

Chapter 1: Plants have a wide array of defenses against herbivores and hence they must confer trade-offs between growth and defense related traits. By using Solanum as a model genus, the major plant defense fitness trade-off hypotheses are reviewed in this chapter along with their assumptions and examples.

Chapter 2: This chapter provides an overview of the enormous intra-specific diversity in trichome morphology in tomato, Solanum lycopersicum. Also, the trichome sub-types are analyzed for the variations in density and dimensions in 10 varieties of tomato by using desktop scanning electron microscope.

Chapter 3: This chapter examines the functional consequences …

Soil Microbial Community Composition Of White Oak Mountain, Tennessee, Matthew Gano, Timothy D. Trott

Research in Biology

Abstract - Soil microbial communities are responsible for nutrient cycling in terrestrial ecosystems and have symbiotic and parasitic relationships with the plant community. However, little is known about the factors that determine the soil microbial community composition. In this study we examined how spring wildflower diversity and geographical factors influence the soil microbial community composition of the second growth oak hickory forests of White Oak Mountain in Southeast Tennessee. The characterization of the soil microbial community was completed with 16S/18S/ITS rDNA amplicon sequencing of total DNA extracted from soil samples that were normalized for each sample plot. Here we characterize …

The Bellarmine Bee Bed: Organizing A Native Plant Garden Using Feedback From The Local Community, Kate Moran

Undergraduate Theses

Animal pollinators are the cornerstone of healthy ecosystems. Their survival is essential for the persistence of entire food chains: from the flowers they cross-pollinate directly, to the animals who depend on those plants for nutrition. The establishment of pollinator gardens—particularly ones that consist of native plants—is an effective way to enhance their biodiversity, abundance, and well-being.

The main goal of this thesis is to construct a pollinator garden that maximizes the benefits for animal pollinators using feedback from local gardeners. A survey was used to gather information about the popularity and preferences of 40 flowering plants, and after analyzing the …

Functional Evaluation Of Two Wetland Sites Within Eastern Michigan University’S Fish Lake Environmental Education Center (Fleec), Brianna M. Rice

2023 Midwest Ecology & Evolution Conference

Wetland ecosystems function as reservoirs of biodiversity for both terrestrial and aquatic organisms, while being among the most vulnerable ecosystems to degradation. The quality of two wetlands at Eastern Michigan University’s Fish Lake Environmental Education Center (FLEEC) was measured based on plant diversity, a northern peat moss bog and one of several vernal pools. The EMU Herbarium documents 122 wetland plant species from 44 families at FLEEC, with 55 species expected within the bog and 67 species expected within vernal pools; 32 species are expected to be shared between sites. The metrics used to assess plant community composition were coefficient …

Decadal Patterns Of Adaptation And Trait Variation Of The Dominant Prairie Grass Across The Midwest Rainfall Gradient And Response To Drought, Jack Sytsma

2023 Midwest Ecology & Evolution Conference

Frequent droughts are predicted to impact grasslands but intraspecific variation in response to drought is not well-known. Big bluestem (Andropogon gerardii) is a grass distributed across the Midwest rainfall gradient (MAP500-1200mm/yr) giving rise to climate-adapted ecotypes. This dominant grass is important for cattle forage and conservation. Reciprocal gardens were established in 2009 in ecological communities (driest to wettest: Colby, Hays, Manhattan, KS to Carbondale IL) and measured for over a decade. Rainfall was reduced using rainout shelters in three sites. Cover and biomass were monitored in plots over time, and height, width, photosynthetic rate, SPAD, and reproductive phenology …

Documenting Plant Biodiversity At The Eastern Michigan University Owned Parsons Center For Arts And Sciences, Emily R. Edwards Mrs.

2023 Midwest Ecology & Evolution Conference

Eastern Michigan University (EMU) owns an 86-acre property in northwest Michigan called the Parsons Center for Arts and Sciences. No botanical research has been done at the Center. A comprehensive plant species list will provide important resources for EMU students, all Parsons users (e.g., artists and community members), and county biologists. This research aims to collect and identify as many plant species at the site as possible and create two online, publicly available databases of the plant list and collection. Such a collection will bring awareness to the Parsons Center and add to its botanical value.

Initial field-work was conducted …

Trait Variation In Bryophytes At Karst Cave Entrances, Chenoa Briggs

2023 Midwest Ecology & Evolution Conference

Cave entrances are critical ecosystems in their role as ecotones between the interior cave environment and the surrounding external environment. Bryophytes are highly sensitive to environmental changes and many species found at caves are specialists to those habitats. This study aims to identify how functional traits of bryophytes are affected by the cave environment. Bryophytes and environmental variables were sampled from six cave entrances. Functional trait data was collected from literature. The data from this study supplies information on the functional traits of bryophytes and can provide insight into the effects of environmental pressures on community assembly.

Where The Wild Mushrooms Are: Species And Secondary Metabolite Diversity In Fungal Species On The Saint Mary’S College Campus, Lex K. Gilman

2023 Midwest Ecology & Evolution Conference

Secondary metabolites in fungi have three main ecological functions: protection against predation, communication with other mushrooms, and assistance in pathogenic interactions. These compounds have also led to the common use of mushrooms as food and in traditional Chinese or other medicinal applications. However, there are currently few good resources for identifying mushroom species in North-Central Indiana. In addition, limited research exists on secondary metabolites in mushrooms. The objective of this research was to create a preliminary database of mushroom species at Saint Mary’s College and conduct preliminary research on the secondary metabolites of these local species. Fungal specimens were identified …

Effects Of Heavy Metal Uptake On Growth, Chlorophyll Content, And Calcium Oxalate Crystals In Lemna Minor (Duckweed), Olivia F. Miller

Undergraduate Honors Thesis Projects

Lemna minor L., duckweed, is a common aquatic plant used for the phytoremediation of wastewater. Heavy metal contamination poses a significant issue, and numerous studies have been conducted on the efficiency of L. minor’s hyperaccumulation ability of these metals. Calcium oxalate crystals are an important aspect of the growth and development of L. minor, but how they are influenced by the uptake of heavy metal has not been extensively studied. This study aims to determine the effects that lead, cadmium, and copper have on the formation of calcium oxalate crystals, vegetative growth, and chlorophyll a and b content on L. …

Life Line Spring 2023, Otterbein Biology And Earth Science Department

Life Line - The Biology Department Newsletter

In this Issue:

Feathered Friends Living on Campus; The Hidden Mussels of the Olentangy River; Early Assurance Program Students Graduate from HCOM; Students Present at American Society for Microbiology Microbe 2022 Meeting; Environmental Science Upgrades Its Remote Monitoring Capabilities; Speed!; UK Students Visit Local Attractions; That's a Lottl Axolotls.

Nutrient Resorption And Stoichiometric Characteristics Of Wuyi Rock Tea Cultivars, Dehuang Zhu, Suhong Peng, Wenzhen Liu, Shengjie Yu, Dafeng Hui

Biology Faculty Research

Nutrient resorption is an important strategy for plants to retain critical nutrients from senesced leaves and plays important roles in nutrient cycling and ecosystem productivity. As a main economic crop and soil and water conservation species, Wuyi Rock tea has been widely planted in Fujian Province, China. However, foliar nutrient resorptions of Wuyi Rock tea cultivars have not been well quantified. In this study, three Wuyi Rock tea cultivars (Wuyi Jingui, Wuyi Rougui, and Wuyi Shuixian) were selected in the Wuyishan National Soil and Water Conservation, Science and Technology Demonstration Park. Resorption efficiencies of nitrogen (NRE), phosphorus (PRE), and potassium …

Changes In Metal-Chelating Metabolites Induced By Drought And A Root Microbiome In Wheat, Anne J. Anderson, Joshua M. Hortin, Astrid R. Jacobson, David W. Britt, Joan E. Mclean

Biological Engineering Faculty Publications

The essential metals Cu, Zn, and Fe are involved in many activities required for normal and stress responses in plants and their microbiomes. This paper focuses on how drought and microbial root colonization influence shoot and rhizosphere metabolites with metal-chelation properties. Wheat seedlings, with and without a pseudomonad microbiome, were grown with normal watering or under water-deficit conditions. At harvest, metal-chelating metabolites (amino acids, low molecular weight organic acids (LMWOAs), phenolic acids, and the wheat siderophore) were assessed in shoots and rhizosphere solutions. Shoots accumulated amino acids with drought, but metabolites changed little due to microbial colonization, whereas the active …

Detection And Capture Of Nitrogen Fixing Bacteria From Soil And Roots Using Plant Specific Lectins, Alana Horton

Natural Sciences and Mathematics | Biological Sciences Master's Theses

Diazotrophs increase plant productivity by providing ammonia directly to plants in the rhizosphere or through association with the roots (Sun et al., 2021). Leveraging diazotrophs can aid in the reduction or application of harmful synthetic nitrogen fertilizers. This thesis aims to use plant root proteins, or lectins, to more rapidly recover viable diazotrophs that are likely to associate with crops. In this study, lectins, are used to recover bacteria from bulk soil by taking advantage of the binding relationship between lectins and bacterial cell surface polysaccharides. When commercially-available lectins were used with a magnetic pulldown to separate the lectin, bacterial …