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Articles 991 - 1020 of 2751

Full-Text Articles in Biology

Molecular Structure Vibrational & Electronic Properties Of Some Isatin Derivatives, Fatma K. Fkandermili Prof, Fatma M. Aldibashi S, Hakan S. Sayiner Prof Mar 2021

Molecular Structure Vibrational & Electronic Properties Of Some Isatin Derivatives, Fatma K. Fkandermili Prof, Fatma M. Aldibashi S, Hakan S. Sayiner Prof

Karbala International Journal of Modern Science

Abstract

In this study, Natural Bonding Orbitals (NBOs) were applied to isatin, 5‑fluoroisatin, 5‑chloroisatin, 5‑methylisatin and 5‑methoxyisatin using the Lee-Yang-Parr correlation functional B3LYP with 6‑311++G(2d,2p) basis set. Natural bonding analysis was performed to consider the transfer interactions of intra-molecular charge, pre-hybridization and electron density within the isatin, 5‑fluoroisatin, 5‑chloroisatin, 5‑methylisatin and 5‑methoxyisatin. In natural bonding orbital analysis, the wave functions of the electrons were explicated in sets of occupied Lewis type terms, (bonds or lone pairs) and sets of unoccupied non‑Lewis (anti‑bond and Rydberg) localized natural bonding orbitals. The electron density between these orbitals was correlated to stabilize the interaction …


Editorial Board Mar 2021

Editorial Board

Karbala International Journal of Modern Science

No abstract provided.


Shorebird Response To Human-Induced Changes At Three Pinellas County Beaches, Rebecca J. Ruthberg-Campagna Mar 2021

Shorebird Response To Human-Induced Changes At Three Pinellas County Beaches, Rebecca J. Ruthberg-Campagna

USF Tampa Graduate Theses and Dissertations

Worldwide, shorebird habitat is being destroyed and degraded by development and sea level rise. Shorebirds depend on availability of pristine, undisturbed coastal habitats for resting and feeding during migration as well as for reproduction. Migratory shorebirds using the East Atlantic Flyway visit the Gulf of Mexico Beaches of Pinellas County, Florida as a stopover site during Fall and Spring migration. In addition to hosting migratory species, Pinellas County beaches are home to several year-round resident species that breed during Summer. Pinellas County is the most densely populated county in Florida, and its Gulf Coast is heavily developed with commercial and …


Neglected Nematodes: The Neurolenin Solution, Kevin M. Shea, Steven Williams Mar 2021

Neglected Nematodes: The Neurolenin Solution, Kevin M. Shea, Steven Williams

Chemistry: Faculty Publications

Over 1 billion people worldwide are affected by Neglected Topical Diseases (NTDs). This group of pathogens encapsulates viruses, bacteria and parasites, and disproportionately affects the world’s poorest and most vulnerable communities in Africa, Asia and Latin America. Lymphatic Filariasis (LF) is an NTD caused by a parasitic nematode infection. The larvae of these filarial nematode worms are transmitted via mosquito bites and disrupt the functioning of the infected individual’s lymphatic system. LF can be caused by three types of filarial worms, Wuchereria bancrofti, Brugia malayi and Brugia timori, which are transmitted by the Culex, Anopheles and Aedes mosquito families. Symptomatic …


College Of Natural Sciences Newsletter, March 2021, College Of Natural Sciences Mar 2021

College Of Natural Sciences Newsletter, March 2021, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 2. Issue 3

Contents:

Page 1 Dean's Message
Page 2-3 Awards and Recognition
Page 4 Student Club Spotlight: MICROBIOLOGY CLUB
Page 5 Research Spotlight: Dr. Chakravarty
Page 6 Open PRAIRIE Data; Grants Awarded in CNS; Research Collquia
Page 7 A Busy Month in Review; Media Coverage of the College


The Reemergence Of Eradicated Disease Due To Ecological Impact Of Climate Change, Claudia Kolakowski Feb 2021

The Reemergence Of Eradicated Disease Due To Ecological Impact Of Climate Change, Claudia Kolakowski

Theses and Dissertations

Global warming is radically changing aspects of the Earth. As scientists continue to research the effects, the ramifications of melting permafrost is coming to light. We build off of a previously existing Anthrax model in the hopes to include climate change as a factor in Anthrax spread. Chapter II develops a simplified version of an Anthrax model. Parameters for the model are found by using previous research and eigenvalues are analyzed in order to find thresholds and equilibria. Chapter III consider the general solutions of the model through eigenvalues and eigenvectors. This model is then extended to include a parameter …


The Influence Of Juvenile Dinosaurs On Community Structure And Diversity, Katlin Schroeder, S. Kathleen Lyons, Felisa A. Smith Feb 2021

The Influence Of Juvenile Dinosaurs On Community Structure And Diversity, Katlin Schroeder, S. Kathleen Lyons, Felisa A. Smith

School of Biological Sciences: Faculty Publications

Despite dominating biodiversity in the Mesozoic, dinosaurs were not speciose. Oviparity constrained even gigantic dinosaurs to less than 15 kg at birth; growth through multiple morphologies led to the consumption of different resources at each stage. Such disparity between neonates and adults could have influenced the structure and diversity of dinosaur communities. Here, we quantified this effect for 43 communities across 136 million years and seven continents. We found that megatheropods (more than 1000 kg) such as tyrannosaurs had specific effects on dinosaur community structure. Although herbivores spanned the body size range, communities with megatheropods lacked carnivores weighing 100 to …


The C. Neoformans Cell Wall: A Scaffold For Virulence, Christine Chrissian Feb 2021

The C. Neoformans Cell Wall: A Scaffold For Virulence, Christine Chrissian

Dissertations, Theses, and Capstone Projects

Cryptococcus neoformans is a globally distributed opportunistic fungal pathogen and the causative agent of life threatening cryptococcal meningoencephalitis in immunocompromised individuals, resulting in ~180,000 deaths each year worldwide. A primary virulence-associated trait of this organism is the production of melanin. Melanins are a class of diverse pigments produced via the oxidation and polymerization of aromatic ring compounds that have a characteristically complex, heterogenous, and amorphous structure. They are synthesized by representatives of all biological kingdoms and share a multitude of remarkable properties such as the ability to absorb ultraviolet (UV) light and protect against ionizing radiation. Melanin production in fungi …


Niche Partitioning Of Low-Light Adapted Prochlorococcus Subecotypes Across Oceanographic Gradients Of The North Pacific Subtropical Front, Anne W. Thompson, Kathleen Kouba, Nathan A. Ahlgren Jan 2021

Niche Partitioning Of Low-Light Adapted Prochlorococcus Subecotypes Across Oceanographic Gradients Of The North Pacific Subtropical Front, Anne W. Thompson, Kathleen Kouba, Nathan A. Ahlgren

Biology Faculty Publications and Presentations

The cyanobacterium Prochlorococcus is the most abundant photosynthetic cell on Earth and contributes to global ocean carbon cycling and food webs. Prochlorococcus is known for its extensive diversity that falls into two groups of ecotypes, the low‐light (LL) and high‐light (HL) adapted ecotypes. Previous work has shown niche partitioning of the very abundant HL adapted ecotypes and subecotypes across oceanographic gradients including temperature, nutrients, and day length. However, niche partitioning of subecotypes within the LL adapted ecotypes has not been studied as well because they are less abundant and less accessible than surface, HL populations. Through high‐throughput, cyanobacterial‐specific, sequencing of …


Thermal Acclimation Of Tropical Coral Reef Fishes To Global Heat Waves, Jacob L. Johansen, Lauren E. Nadler, Adam Habary, Alyssa J. Bowden, Jodie Rummer Jan 2021

Thermal Acclimation Of Tropical Coral Reef Fishes To Global Heat Waves, Jacob L. Johansen, Lauren E. Nadler, Adam Habary, Alyssa J. Bowden, Jodie Rummer

Marine & Environmental Sciences Faculty Articles

As climate-driven heat waves become more frequent and intense, there is increasing urgency to understand how thermally sensitive species are responding. Acute heating events lasting days to months may elicit acclimation responses to improve performance and survival. However, the coordination of acclimation responses remains largely unknown for most stenothermal species. We documented the chronology of 18 metabolic and cardiorespiratory changes that occur in the gills, blood, spleen, and muscles when tropical coral reef fishes are thermally stressed (+3.0°C above ambient). Using representative coral reef fishes (Caesio cuning and Cheilodipterus quinquelineatus) separated by >100 million years of evolution and …


The Effect Of Barometric Pressure, Temperature, And Precipitation On Preterm Labor In Expecting Women In South Carolina, Allison K. Fletcher Jan 2021

The Effect Of Barometric Pressure, Temperature, And Precipitation On Preterm Labor In Expecting Women In South Carolina, Allison K. Fletcher

Journal of the South Carolina Academy of Science

Each year, approximately 9.9% of infants in the United States are born prematurely at less than 37 weeks of gestation with unidentified causes. From 2014 to 2016, the total preterm birth rate rose 3%, and late preterm birth rates rose almost 4%. One source of preterm labor that has been examined in recent years is its potential correlation with meteorological phenomena, including barometric pressure, temperature, and precipitation. In September of 2019, birth data recording 322 deliveries from two South Carolina hospitals was collected along with weather data on the given dates. The changes in temperature, barometric pressure, and precipitation for …


The Effect Of Alcalase Concentration On The Proteins From The Shells Of Litopenaeus Setiferus (White Shrimp), Liam T. Quan Jan 2021

The Effect Of Alcalase Concentration On The Proteins From The Shells Of Litopenaeus Setiferus (White Shrimp), Liam T. Quan

Journal of the South Carolina Academy of Science

Chitin is a naturally abundant polymer that also happens to be biodegradable. Chitin can be used in a variety of different products such as biodegradable plastics, papers, medical products, foods, and medical treatments. To extract chitin, shells must be demineralized and deproteinized. The goal of this experiment was to examine the effect of the protease Alcalase in the deproteinization of litopenaeus setiferus shells. The hypothesis was that if the concentration of Alcalase increased, then the absorbance of proteins in the spectrophotometer reading would increase. The null hypothesis was that if the concentration increased there would be no change in absorption. …


Mussel Musings On The Powell River, Aggy Vanderpool Jan 2021

Mussel Musings On The Powell River, Aggy Vanderpool

Cumberland Mountain Naturalist

No abstract provided.


Regulation Of Kv11.1 Isoform Expression By Polyadenylate Binding Protein Nuclear 1, Matthew R. Stump, Rachel T. Nguyen, Rachel H. Drgastin, Delaney Search, Quiming Gong, Zhengfeng Zhou Jan 2021

Regulation Of Kv11.1 Isoform Expression By Polyadenylate Binding Protein Nuclear 1, Matthew R. Stump, Rachel T. Nguyen, Rachel H. Drgastin, Delaney Search, Quiming Gong, Zhengfeng Zhou

Faculty Publications - Department of Biological & Molecular Science

The Kv11.1 voltage-gated potassium channel, encoded by the KCNH2 gene, conducts the rapidly activating delayed rectifier current in the heart. KCNH2 pre-mRNA undergoes alternative polyadenylation to generate two C-terminal Kv11.1 isoforms in the heart. Utilization of a poly(A) signal in exon 15 produces the full-length, functional Kv11.1a isoform, while intron 9 polyadenylation generates the C-terminally truncated, nonfunctional Kv11.1a-USO isoform. The relative expression of Kv11.1a and Kv11.1a-USO isoforms plays an important role in the regulation of Kv11.1 channel function. In this study, we tested the hypothesis that the RNA polyadenylate binding protein nuclear 1 (PABPN1) interacts with a unique 22 nt …


A Record Of Vapour Pressure Deficit Preserved In Wood And Soil Across Biomes, Adrian Broz, Gregory J. Retallack, Toby M. Maxwell, Lucas C.R. Silva Jan 2021

A Record Of Vapour Pressure Deficit Preserved In Wood And Soil Across Biomes, Adrian Broz, Gregory J. Retallack, Toby M. Maxwell, Lucas C.R. Silva

Biology Faculty Publications and Presentations

The drying power of air, or vapour pressure deficit (VPD), is an important measurement of potential plant stress and productivity. Estimates of VPD values of the past are integral for understanding the link between rising modern atmospheric carbon dioxide (pCO2) and global water balance. A geological record of VPD is needed for paleoclimate studies of past greenhouse spikes which attempt to constrain future climate, but at present there are few quantitative atmospheric moisture proxies that can be applied to fossil material. Here we show that VPD leaves a permanent record in the slope (S) of least-squares …


The Effects Of Endocrine Disrupting Chemicals On Plants, Christian Mathew Pridemore Jan 2021

The Effects Of Endocrine Disrupting Chemicals On Plants, Christian Mathew Pridemore

Theses, Dissertations and Capstones

Endocrine disrupting chemicals (EDCs) are a diverse group of chemicals which can have a detrimental effect on human health, affecting any physiological body system sensitive to hormonal changes. Human exposure to EDCs is inevitable due to their prevalence in modern living; EDCs are integral compounds found in everyday items with which humans interact and ingest. These, along with other indirect avenues leading to EDC exposure, pose a threat to both human health and to the health of wildlife and agricultural environments. Studies on the effects of EDCs on plants can be substantially informative and necessary for progress towards solving the …


Understanding Patterns And Functional Impacts Of An Invasive Tree And Its Biological Control In A Riparian System, Annie L. Henry Jan 2021

Understanding Patterns And Functional Impacts Of An Invasive Tree And Its Biological Control In A Riparian System, Annie L. Henry

Electronic Theses and Dissertations

Invasive species have become an inextricable part of the landscape, particularly in riparian plant communities, and removal is often a key component of restoration programs. Biological control (biocontrol) is a method of removal that is often both efficient and effective. However, the impact of biocontrol on target species and indirect effects from invasive species removal can be hard to predict. While monitoring the impact of invasive species removal usually involves some species-based assessment such as changes in diversity, historically dominant species or native species, these strategies do not typically provide insight into the mechanisms underlying plant community response to removal. …


Synthetic Pathways For Potential Platinum 1,10-Phenanthroline Compounds, Jacob Young Jan 2021

Synthetic Pathways For Potential Platinum 1,10-Phenanthroline Compounds, Jacob Young

Mahurin Honors College Capstone Experience/Thesis Projects

There are extremely important uses for platinum when it comes to the treatment of cancers and dementias. One potential therapeutic combines platinum with a structure known as phenanthroline. This platinum phenanthroline structure is normally extremely water-insoluble, rendering it difficult to study and use in biological systems. The Williams’ Biochemistry Laboratory endeavored to find a simpler synthetic pathway for a water-soluble phenanthroline product. While unsuccessful in identifying a product as of yet, many synthetic pathways have been ruled out, and more hypothetical pathways are being formulated for testing.

Among the strategies analyzed are varying solvent, reagents, apparatus, methodology, and temperature in …


Acoustic Communication In The Atlantic Mudskipper, Periopthalmus Barbarus, Seth Hoffman Jan 2021

Acoustic Communication In The Atlantic Mudskipper, Periopthalmus Barbarus, Seth Hoffman

Mahurin Honors College Capstone Experience/Thesis Projects

Mudskippers are intertidal fishes that can survive both in and out of the water. They are territorial and exhibit behaviors to attract mates and defend against competitors. Recently, it has been shown that vibrations are produced and transmitted through the mud during such displays in one species, Periophthalmodon septemradiatus. To see if similar vibrations are produced in other species of mudskippers, I recorded the behavioral interactions of pairs of the mudskipper Periophthalmus barbarus via digital video and acoustic signals via accelerometer. Comparative analysis of fish mass, sex, contest length, and contest outcome coupled with acoustic characteristics within dyadic pairings …


Antibiotic Resistant Gene Concentrations In Bacteria In Groundwater From Agricultural Waste, Koji Barnaby Jan 2021

Antibiotic Resistant Gene Concentrations In Bacteria In Groundwater From Agricultural Waste, Koji Barnaby

Mahurin Honors College Capstone Experience/Thesis Projects

The overuse of antibiotics has led to an increase in antibiotic resistant bacteria since some antibiotics are sent into the wastewater system by waste produced by both humans and agriculture, making exposure to these bacteria more likely. The evolution of these bacteria in groundwater is of particular concern, as groundwater is used as a source of drinking water, and infections by these bacteria would be more difficult to treat. There are no policies in place to monitor or regulate antibiotic resistance bacteria in groundwater, leaving the threat to public health unknown. The study area of Crumps Cave in Smiths Grove, …


A Review And Comparison Of U.S. State Wildlife Action Plans For Stonefly (Insecta, Plecoptera) Species Of Greatest Conservation Need, Kathryn Greene Jan 2021

A Review And Comparison Of U.S. State Wildlife Action Plans For Stonefly (Insecta, Plecoptera) Species Of Greatest Conservation Need, Kathryn Greene

Mahurin Honors College Capstone Experience/Thesis Projects

State Wildlife Action Plans (SWAPs) were created in order to identify Species of Greatest Conservation Need (SGCN) and develop plans to prevent their populations from further decline and the possibility of being listed as threatened or endangered. Which SGCNs are included are decided based on characteristics that determine their vulnerability. As of early 2021, approximately 720stoneflies (Insecta, Plecoptera) species are found in the U.S., but only 143 are listed as SGCN. Only 29 states have stoneflies included on their SGCN lists, but 37 states use EPT (Ephemeroptera + Plecoptera + Trichoptera) metrics when assessing water quality issues in running water …


Characterization Of Microbial Populations In Landfill Leachate, Umut M. Bicim, Hanna Fulford, Lori A. Hoagland, Alejandro R. Sanchez, Amisha D. Shah, Inez Hua Jan 2021

Characterization Of Microbial Populations In Landfill Leachate, Umut M. Bicim, Hanna Fulford, Lori A. Hoagland, Alejandro R. Sanchez, Amisha D. Shah, Inez Hua

Discovery Undergraduate Interdisciplinary Research Internship

In the United States, municipal solid waste (MSW) landfills remain a potential mining source of recoverable materials, including but not limited to critical, precious, and rare earth metals found in electronic waste. This is possible due to collectible leachate that filters through MSW landfills, carrying metals, nutrients of value, and microbes—some of which may hold key metal bioleaching properties—within. The purpose of this study is to begin analyzing leachate from MSW landfills in the American Midwest to understand the composition of microbial communities within these landfills. Landfill leachate samples sourced in northern Indiana, representing the landfill process during unique times …


Effects Of Salt Runoff On Aquatic Macroinvertebrate Colonies, Kendra Fink Jan 2021

Effects Of Salt Runoff On Aquatic Macroinvertebrate Colonies, Kendra Fink

Journal of Earth and Life Science

Aquatic macroinvertebrates bridge the gap in part between primary and secondary consumers, making them ecologically important for most aquatic systems. They are used as biotic indicators for water quality due to their sensitivity to pollutants and may be affected, specifically, by chloride ion concentrations in the water. Water quality can be evaluated by looking at species compositions and their respective tolerance values. To determine long-term effects of road salt on macroinvertebrate colonies, a total of 338 identified macroinvertebrates of 10 families (two generalized to class) at 9 sites were collected from before, after, and between three bridges on Turtle River …


The Effects Of Saline Soil On Microbiome And The Isolation Of Root-Associated Microbes To Relieve Salinity Stress, Duncan Jakubowski Jan 2021

The Effects Of Saline Soil On Microbiome And The Isolation Of Root-Associated Microbes To Relieve Salinity Stress, Duncan Jakubowski

Electronic Theses and Dissertations

Increasing levels of salinity in once-viable lands for crop production is a serious and growing problem in the Northern Great Plains. The objectives of this study were to determine the effects of saline soil on the microbial composition of plant roots and bulk soil, to measure metabolic changes in plant roots from saline soil, to determine the viability of root-associated microbes as inoculants to increase stress tolerance in plants, as well as determine the impact of saline soil on nitrogen cycling genes linked to greenhouse gas production. This study hypothesizes that high soil salinity levels have a significant impact on …


Breeding And Feeding Habitat Selection By An Island Endemic Bird May Increase Its Vulnerability To Climate Change, Paul Radley, Eddie Van Etten, David Blake, Robert Davis Jan 2021

Breeding And Feeding Habitat Selection By An Island Endemic Bird May Increase Its Vulnerability To Climate Change, Paul Radley, Eddie Van Etten, David Blake, Robert Davis

Research Datasets

Characterising patterns of habitat use is an important first step for effective conservation planning. Species restricted to low-lying islands are at greatest risk from climate change-related sea level rise, and requirements for breeding and foraging habitat may determine their risk from tidal inundation. The endangered Micronesian Scrubfowl (Megapodius laperouse senex) is a model species for understanding these impacts. This species faces the cumulative challenges of tourist visitation, invasive species, and rising sea levels, yet little is understood about its habitat use in the Rock Islands Southern Lagoon Conservation Area (RISL) of Palau. We studied the habitat requirements of …


Use Of Lymesim 2.0 To Assess The Potential For Single And Integrated Management Methods To Control Blacklegged Ticks (Ixodes Scapularis; Acari: Ixodidae) And Transmission Of Lyme Disease Spirochetes, Shravani Chitineni, Elizabeth R. Gleim, Holly D. Gaff Jan 2021

Use Of Lymesim 2.0 To Assess The Potential For Single And Integrated Management Methods To Control Blacklegged Ticks (Ixodes Scapularis; Acari: Ixodidae) And Transmission Of Lyme Disease Spirochetes, Shravani Chitineni, Elizabeth R. Gleim, Holly D. Gaff

Undergraduate Honors Theses

Annual Lyme disease cases continue to rise in the U.S. making it the most reported vector-borne illness in the country. The pathogen (Borrelia burgdorferi) and primary vector (Ixodes scapularis; blacklegged tick) dynamics of Lyme disease are complicated by the multitude of vertebrate hosts and varying environmental factors, making models an ideal tool for exploring disease dynamics in a time- and cost-effective way. In the current study, LYMESIM 2.0, a mechanistic model, was used to explore the effectiveness of three commonly used tick control methods: habitat-targeted acaricide (spraying), rodent-targeted acaricide (bait boxes), and white-tailed deer targeted acaricide (4-poster …


Residual Spatial Autocorrelation In Macroecological And Biogeographical Modeling: A Review, Guetchine Gaspard Jan 2021

Residual Spatial Autocorrelation In Macroecological And Biogeographical Modeling: A Review, Guetchine Gaspard

Theses and Dissertations--Geography

Macroecological and biogeographical modelers have predicted the distribution of species across space relying on the relationship between biotic processes and environmental variables. Such a method employs data associated, for instance, with species abundance or presence/absence, climate, geomorphology, and soils. Statistical analyses found in previous studies have highlighted the importance of accounting for the effects of spatial autocorrelation (SAC), which indicates a level of dependence between pairs of nearby observations. A consensus has existed that residual spatial autocorrelation (rSAC) can substantially impact modeling processes and inferences. However, more emphasis should be put on identifying the sources of rSAC and the degree …


College Of Natural Sciences 2021 Year-End Publication, College Of Natural Sciences Jan 2021

College Of Natural Sciences 2021 Year-End Publication, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Page 2 Welcome, Table of Contents
Page 3 Our Departments: Highlights From 2021
Page 4 Overview of the College
Page 5 Overview of Bold & Blue
Pages 6, 7 Announcing the First Endowed Professorship in the College of Natural Sciences
Page 8 Spooky Science, Astronomy Outreach, SDSU Chemistry Research Features on Journal Cover
Page 9 Students Win Business Plan Competition With Aims to Improve Shelf Life of Produce, Summer Researchers
Page 10 NIH Grant Helps Unravel Rare Inflammatory Genetic Disorders, International Excellence in South Dakota
Page 11 The Next Frontier of Science Education: Virtual Reality, SDSU Researchers Uncover how Cancer …


An Investigation Into Historical And Contemporary Breeding Occurrence Of The Ferruginous Hawk In Kansas, Erica Clark Jan 2021

An Investigation Into Historical And Contemporary Breeding Occurrence Of The Ferruginous Hawk In Kansas, Erica Clark

Master's Theses or Doctor of Nursing Practice

The Ferruginous Hawk (Buteo regalis) is the largest Buteo species occurring in grasslands, nesting along bluffs, buttes and isolated trees. In Kansas, the Ferruginous Hawk is listed as a Species of Greatest Conservation Need, Tier II. A previous study on Ferruginous Hawks nesting in Kansas during the years of 1979 to 1987, with sporadic visits from the 1990s to 2000, revealed that the most productive nesting territories were inaccessible to predators, placed on rocky ledges and the surrounding landscape was over 50% rangeland. I revisited 82 of the 111 historic nest territories in the summers of 2019 and …


Development Of Environmental Metabarcoding Analysis For Use In Ecological Studies Of Aquatic Fungal And Oomycete Communities Using Nanopore Sequencing, Douglas Bennett Jan 2021

Development Of Environmental Metabarcoding Analysis For Use In Ecological Studies Of Aquatic Fungal And Oomycete Communities Using Nanopore Sequencing, Douglas Bennett

All Master's Theses

One of several factors contributing to amphibian decline are organisms called water molds (oomycetes), which parasitize and kill amphibian and fish eggs, larvae, and occasionally adults. Amphibian decline is one of the most difficult issues facing ecologists and conservationists to date; half of amphibian species are in severe decline and one third of amphibian species are facing extinction, threatening the stability of ecosystems globally. Multiple oomycete species are known to cause the disease saprolegniasis and contribute to amphibian decline; however, the full range of species involved is not presently known. This study evaluated the Oxford Nanopore minION DNA sequencer (R9.2 …