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Full-Text Articles in Biology

Artificial Intelligence For Para Rubber Identification Combining Five Machine Learning Methods, Chairote Yaiprasert Ph.D. Dec 2021

Artificial Intelligence For Para Rubber Identification Combining Five Machine Learning Methods, Chairote Yaiprasert Ph.D.

Karbala International Journal of Modern Science

This study aims to identify Para rubber species using a combination of five machine learning techniques to classify leaf images. The learning process is defined using a dataset for each classification method. Approximately 1,472 leaf images are prepared consisting of various sizes, shapes, quality provided for the model. The classification indicators are defined with the help of an algorithm to identify at least three of the top five potential classification outcomes. The algorithm accurately predicts 100% of the five classification methods. Methods can provide precise and rapid classification of large quantities, without the need for image preprocessing prior to classification.


Synergistic Extraction Of Silver From Nitric Acid Medium With Dithizone In The Presence Of A Secondary Amine- Amberlite La-2 Using 110mag-Radioisotope, Madhusudan Mandal Ph.D. Dec 2021

Synergistic Extraction Of Silver From Nitric Acid Medium With Dithizone In The Presence Of A Secondary Amine- Amberlite La-2 Using 110mag-Radioisotope, Madhusudan Mandal Ph.D.

Karbala International Journal of Modern Science

Synergistic extraction of silver has been investigated in the presence of two different kinds of extractants, one of which is a chelating ligand, dithizone and the other is a long-chain secondary amine amberlite LA-2 at pH 3.0 using a radiotracer technique. The apparent formation constant, overall equilibrium constant and adduct formation constants were calculated from distribution coefficients. Interestingly, it was observed that the adduct formation constant is too high when the ligand concentration is increased by keeping the amine concentration fixed at 0.044 M compared to that obtained when the ligand concentration is kept fixed at 8.74×10-4 M.


Editorial Board Dec 2021

Editorial Board

Karbala International Journal of Modern Science

No abstract provided.


(R1468) Global Analysis Of An Seirs Model For Covid-19 Capturing Saturated Incidence With Treatment Response, David A. Oluyori, Helen O. Adebayo, Ángel G.C. Pérez Dec 2021

(R1468) Global Analysis Of An Seirs Model For Covid-19 Capturing Saturated Incidence With Treatment Response, David A. Oluyori, Helen O. Adebayo, Ángel G.C. Pérez

Applications and Applied Mathematics: An International Journal (AAM)

In this work, a new SEIRS model with saturated incidence rate and piecewise linear treatment response is proposed to describe the dynamics of COVID-19. It is assumed that the treatment response is proportional to the number of infected people as long as the incidence cases are within the capacity of the healthcare system, after which the value becomes constant, when the number of confirmed cases exceeds the carrying capacity of the available medical facilities. Thus, the basic reproduction number of the model is obtained. It is proved that the disease-free equilibrium is globally asymptotically stable when the basic reproduction number …


(R1488) Transformation Of Glucokinase Under Variable Rate Constants And Thermal Conditions: A Mathematical Model, Mukhtar Ahmad Khanday, Roohi Bhat Dec 2021

(R1488) Transformation Of Glucokinase Under Variable Rate Constants And Thermal Conditions: A Mathematical Model, Mukhtar Ahmad Khanday, Roohi Bhat

Applications and Applied Mathematics: An International Journal (AAM)

The glucokinase (GK) in cells plays a pivotal role in the regulation of carbohydrate metabolism and acts as a sensor of glucose. It helps us to control glucose levels during fast and food intake conditions through triggering shifts in metabolism or cell functions. Various forms of hypoglycaemia and hyperglycaemia occur due to the transformations of the gene of the Glucokinase. The mathematical modelling of enzyme dynamics is an emerging research area to serve its role in biological investigations. Thus, it is imperative to establish a mathematical model to understand the kinetics of native and denatured forms of enzyme-GK under thermal …


Predicting Lifespan Of Drosophila Melanogaster: A Novel Application Of Convolutional Neural Networks And Zero-Inflated Autoregressive Conditional Poisson Model, Yi Zhang, V. A. Samaranayake, Gayla R. Olbricht, Matthew S. Thimgan Dec 2021

Predicting Lifespan Of Drosophila Melanogaster: A Novel Application Of Convolutional Neural Networks And Zero-Inflated Autoregressive Conditional Poisson Model, Yi Zhang, V. A. Samaranayake, Gayla R. Olbricht, Matthew S. Thimgan

Mathematics and Statistics Faculty Research & Creative Works

A model to classify the lifespan of Drosophila, the fruit fly, into short- and long-lived categories based on a sleep characteristic, extracted from activity data, is developed using a two-stage process. Stage 1 models the per-minute activity counts of each fly using a zero-inflated autoregressive conditional Poisson model. These probabilities are allowed to vary hourly, reflecting the circadian and other cycles present in a fly's sleep architecture. A 5-day moving window is used to model data allowing the model parameters to vary over the course of the fly's life. The resulting probabilities capture information about changes in sleep patterns with …


(R1493) Discussion On Stability And Hopf-Bifurcation Of An Infected Prey Under Refuge And Predator, Moulipriya Sarkar, Tapasi Das Dec 2021

(R1493) Discussion On Stability And Hopf-Bifurcation Of An Infected Prey Under Refuge And Predator, Moulipriya Sarkar, Tapasi Das

Applications and Applied Mathematics: An International Journal (AAM)

The paper deals with the case of non-selective predation in a partially infected prey-predator system, where both the susceptible prey and predator follow the law of logistic growth and some preys avoid predation by hiding. The disease-free preys get infected in due course of time by a certain rate. However, the carrying capacity of the predator population is considered proportional to the sum-total of the susceptible and infected prey. The positivity and boundedness of the solutions of the system are studied and the existence of the equilibrium points and stability of the system are analyzed at these points. The effect …


(R1523) Abundant Natural Resources, Ethnic Diversity And Inclusive Growth In Sub-Saharan Africa: A Mathematical Approach, Juliet I. Adenuga, Kazeem B. Ajide, Anthonia T. Odeleye, Abayomi A. Ayoade Dec 2021

(R1523) Abundant Natural Resources, Ethnic Diversity And Inclusive Growth In Sub-Saharan Africa: A Mathematical Approach, Juliet I. Adenuga, Kazeem B. Ajide, Anthonia T. Odeleye, Abayomi A. Ayoade

Applications and Applied Mathematics: An International Journal (AAM)

The sub-Saharan African region is blessed with abundant natural resources and diverse ethnic groups, yet the region is dominated by the largest number of poor people worldwide due to inequitable distribution of national income. Existing statistics forecast decay in the quality of lives over the years compared to the continent of Asia that shares similar history with the region. In this paper, a-five dimensional first-order nonlinear ordinary differential equations was formulated to give insight into various factors that shaped dynamics of inclusive growth in sub-Saharan Africa. The validity test was performed based on ample mathematical theorems and the model was …


Implications Of Condition Shifts On Shallow Lakes, Alicia Skolte Dec 2021

Implications Of Condition Shifts On Shallow Lakes, Alicia Skolte

Biology Graduate Theses

Shallow lakes have undergone condition shifts throughout time by natural causes, management actions, or a combination of the two. Because shallow lakes are especially vulnerable to shifts towards turbid conditions, management actions on shallow lakes have focused on water level/retainment, biomanipulations, the management/removal of non-native species, and land-use changes. This review compiles a summary on ecosystem shifts including a discussion on the frequency of shifts historically and the importance and causes of those shifts, trophic cascades, and the influence of introduced species in shallow lakes. This review will then examine case-studies of management-linked shifts and explain current shift theory. Next, …


Feeding Patterns And Diet Overlap Of Muskellunge And Co-Occurring Piscivores In Minnesota Lakes, Kamden Glade Dec 2021

Feeding Patterns And Diet Overlap Of Muskellunge And Co-Occurring Piscivores In Minnesota Lakes, Kamden Glade

Biology Graduate Theses

Muskellunge Esox masquinongy are the largest member of the family Esocidae found in Minnesota and are managed for trophy angling opportunities with large minimum size requirements, limited harvest, and stocking to support existing populations or expand angling opportunities. While Muskellunge impacts at the community level appear minimal based on available literature, relatively little is known about Muskellunge diets, eat the le particularly in Minnesota. In this study, we used gastric lavage to examine gut contents of Muskellunge, Northern Pike Esox lucius, Walleye Sander vitreus, and Largemouth Bass Micropterus salmoides. Diets were quantified using an index of relative importance (IRI) and …


A Global Agenda For Advancing Freshwater Biodiversity Research, Alain Maasri, Sonja C. Jähnig, Hendrik Freitag, 93 Co-Authors Dec 2021

A Global Agenda For Advancing Freshwater Biodiversity Research, Alain Maasri, Sonja C. Jähnig, Hendrik Freitag, 93 Co-Authors

Biology Faculty Publications

Global freshwater biodiversity is declining dramatically, and meeting the challenges of this crisis requires bold goals and the mobilisation of substantial resources. While the reasons are varied, investments in both research and conservation of freshwater biodiversity lag far behind those in the terrestrial and marine realms. Inspired by a global consultation, we identify 15 pressing priority needs, grouped into five research areas, in an effort to support informed stewardship of freshwater biodiversity. The proposed agenda aims to advance freshwater biodiversity research globally as a critical step in improving coordinated actions towards its sustainable management and conservation.


College Of Natural Sciences Newsletter, December 2021, College Of Natural Sciences Dec 2021

College Of Natural Sciences Newsletter, December 2021, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 2, Issue 12

Page 1 Dean's Message
Page 2 Awards & Recognition
Page 2 Media Coverage of CNS
Page 3 Media Coverage Cont.
Page 4 Science as Art Competition
Page 5 Open PRAIRIE Data
Page 5 Grants Awarded in CNS


(R1412) Stability And Bifurcation Of A Cholera Epidemic Model With Saturated Recovery Rate, Huda Abdul-Satar, Raid K. Naji Dec 2021

(R1412) Stability And Bifurcation Of A Cholera Epidemic Model With Saturated Recovery Rate, Huda Abdul-Satar, Raid K. Naji

Applications and Applied Mathematics: An International Journal (AAM)

In this paper, a Cholera epidemic model is proposed and studied analytically as well as numerically. It is assumed that the disease is transmitted by contact with Vibrio cholerae and infected person according to dose-response function. However, the saturated treatment function is used to describe the recovery process. Moreover, the vaccine against the disease is assumed to be utterly ineffective. The existence, uniqueness and boundedness of the solution of the proposed model are discussed. All possible equilibrium points and the basic reproduction number are determined. The local stability and persistence conditions are established. Lyapunov method and the second additive compound …


Efficacy Of Sediment Contaminant Remediation Of The Benthos In A Segment Of The Southern Branch Of The Elizabeth River, Colton Martin Dec 2021

Efficacy Of Sediment Contaminant Remediation Of The Benthos In A Segment Of The Southern Branch Of The Elizabeth River, Colton Martin

Biological Sciences Theses & Dissertations

The bottom sediment of the Southern Branch of the Elizabeth River, a tributary of Chesapeake Bay, was historically contaminated with hydrocarbons from industrial sources especially wood treatment facilities. The Elizabeth River Project selected a section of the bottom off Money Point in the Southern Branch for a sediment contaminant remediation effort. Prior to initiation of remediation efforts, a survey occurred in summer 2010 to characterize the ecological condition of the benthic communities off Money Point compared to benthic communities of a benthic region across the channel and northwest of Money Point near Blows Creek. That study characterized the benthos of …


Consolidation Of Mobile Sands With Ameliorants And Phytomelioration, Sharibay Toremuratov, Bakhtiyar Ismailov Nov 2021

Consolidation Of Mobile Sands With Ameliorants And Phytomelioration, Sharibay Toremuratov, Bakhtiyar Ismailov

Karakalpak Scientific Journal

The results of even a few experiments show the possibility of obtaining a tangible effect when jointly carrying out chemical reclamation and phytomelioration. Reliable habitation of plants is a guarantee of further long-term stopping of the movement of sand and improvement of the ecological situation.

Thus, the sand-fixing crust obtained by this method simultaneously performs several functions: it stops the movement of sand, reduces dust removal, creates favorable conditions for the growth and development of vegetation, contributes to the emergence of soil structures, the accumulation of humus, etc.


Shifts In The Acute Stress Response In Red-Eared Sliders (Trachemys Scripta) During The Activity Season, Jennifer Lauren Terry Nov 2021

Shifts In The Acute Stress Response In Red-Eared Sliders (Trachemys Scripta) During The Activity Season, Jennifer Lauren Terry

Student Theses and Dissertations

Tradeoffs may occur among biological processes if individuals are energetically challenged. Glucocorticoids (GC) promote essential daily processes and energy reprioritization during stressful or energy-demanding periods and provide insight to an individual’s energetic state. I captured 123 wild adult red-eared sliders (Trachemys scripta) outside of Cherry Valley, Arkansas in the Arkansas Delta between May and October 2019 to assess energy tradeoffs between innate immunity and GC secretion. I collected blood samples at 0–3, 30, and 60 min and used plasma in bacterial killing assays and radioimmunoassays to assess innate immunity and corticosterone concentrations, respectively. Tradeoffs were not evident in this population …


Breeding And Feeding Habitat Selection By An Island Endemic Bird May Increase Its Vulnerability To Climate Change, Paul M. Radley, Eddie J. B. Van Etten, David Blake, Robert A. Davis Nov 2021

Breeding And Feeding Habitat Selection By An Island Endemic Bird May Increase Its Vulnerability To Climate Change, Paul M. Radley, Eddie J. B. Van Etten, David Blake, Robert A. Davis

Research outputs 2014 to 2021

Characterizing patterns of habitat use is an important first step for effective conservation planning. Species restricted to low-lying islands are at greatest risk from climate change-related sea level rise, and requirements for breeding and foraging habitat may determine their risk from tidal inundation. The endangered Micronesian Scrubfowl (Megapodius laperouse senex) is a model species for understanding these impacts. This species faces the cumulative challenges of tourist visitation, invasive species, and rising sea levels, yet little is understood about its habitat use in the Rock Islands Southern Lagoon Conservation Area (RISL) of Palau. We studied the habitat requirements of this mound-nesting …


Image-Based Microbiome Profiling Differentiates Gut Microbial Metabolic States, Sarwesh Rauniyar Nov 2021

Image-Based Microbiome Profiling Differentiates Gut Microbial Metabolic States, Sarwesh Rauniyar

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.


Poliomyelitis Virus Vs Smallpox: An Ordinary Differential Equation Model Comparison, Kate Mcgough Nov 2021

Poliomyelitis Virus Vs Smallpox: An Ordinary Differential Equation Model Comparison, Kate Mcgough

Annual Symposium on Biomathematics and Ecology Education and Research

No abstract provided.


On The Relationship Between Pain Variability And Relief In Randomized Clinical Trials, Siddharth Tiwari '22 Nov 2021

On The Relationship Between Pain Variability And Relief In Randomized Clinical Trials, Siddharth Tiwari '22

Student Publications & Research

Previous research suggests greater baseline variability is associated with greater pain relief in those who receive a placebo. However, studies that evidence this association do not control for confounding effects (natural history and regression-to-the-mean); for this reason, we analyzed data from two randomized clinical trials (Placebo I and Placebo II, N = 134) while adjusting for confounding effects via a no-treatment group. Results agree between the two placebo groups: both placebo groups showed a negligible correlation between baseline variability and adjusted response (r sp (CI 95% ) = 0.13 (−0.09, 0.37) and 0.01 (−0.15, 0.20) for Placebo I and II, …


On The Relationship Between Pain Variability And Relief In Randomized Clinical Trials, Siddharth Tiwari '22 Nov 2021

On The Relationship Between Pain Variability And Relief In Randomized Clinical Trials, Siddharth Tiwari '22

Student Publications & Research

Previous research suggests greater baseline variability is associated with greater pain relief in those who receive a placebo. However, studies that evidence this association do not control for confounding effects (natural history and regression-to-the-mean); for this reason, we analyzed data from two randomized clinical trials (Placebo I and Placebo II, N = 134) while adjusting for confounding effects via a no-treatment group. Results agree between the two placebo groups: both placebo groups showed a negligible correlation between baseline variability and adjusted response (r sp (CI 95% ) = 0.13 (−0.09, 0.37) and 0.01 (−0.15, 0.20) for Placebo I and II, …


College Of Natural Sciences Scholarship Brunch Program, November 6th 2021, College Of Natural Sciences Nov 2021

College Of Natural Sciences Scholarship Brunch Program, November 6th 2021, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

This is the program for the first annual College of Natural Sciences Scholarship Brunch, held on November 6th, 2021 at McCrory Gardens.


College Of Natural Sciences Newsletter, November 2021, College Of Natural Sciences Nov 2021

College Of Natural Sciences Newsletter, November 2021, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 2, Issue 11

Page 1 Dean's Message
Page 2 Awards & Recognition
Page 3 Media Coverage of CNS
Page 4 2021 Day of Scholars
Page 5 Astronomy Outreach
Page 6 SDSU Faculty & Students Host American Society for Microbiology Annual Conference
Page 7 Scholarship Brunch
Page 8 Open PRAIRIE Data, SI Final Exam Review Sessions
Page 9 Aamlid Family Anatomy Lab Photos


Studying Neurotoxic Effects Of Three Synthetic Insecticides And Two Herbal Infusions On The Nervous System Of The Fruit Fly Drosophila Melanogaster, Sara Khamis Obaid Hamarain Al Dhaheri Nov 2021

Studying Neurotoxic Effects Of Three Synthetic Insecticides And Two Herbal Infusions On The Nervous System Of The Fruit Fly Drosophila Melanogaster, Sara Khamis Obaid Hamarain Al Dhaheri

Theses

Pesticides applied on plants and animals can reach the human diet once they enter food chains. Insecticides are neurotoxic agents; they have the potential of causing damage to the nervous system in people. The damage could induce or promote neurodegenerative diseases such as the Parkinson’s and Alzheimer's. It is well-documented that the exposure to the insecticide chlorpyrifos (CPF) stimulate the Parkinson disease in humans and experimental animals. Thus, this research project will study effect of three synthetic insecticides pirimicarb, tefluthrin, and CPF on the nervous system of Drosophila melanogaster as a model organism. In addition, the project will evaluate the …


Overfishing Drives Over One-Third Of All Sharks And Rays Toward A Global Extinction Crisis, Nicholas K. Dulvy, Nathan Pacoureau, Cassandra L. Rigby, Riley A. Pollom, Rima W. Jabado, David A. Ebert, Brittany Finucci, Caroline M. Pollock, Jessica Cheok, Danielle H. Derrick, Katelyn B. Herman, C. Samantha Sherman, Wade J. Vanderwright, Julia M. Lawson, Rachel H.L. Walls, John K. Carlson, Patricia Charvet, Kinattumkara K. Bineesh, Daniel Fernando, Gina M. Ralph, Jay H. Matsushiba, Craig Hilton-Taylor, Sonja V. Fordham, Colin A. Simpfendorfer Nov 2021

Overfishing Drives Over One-Third Of All Sharks And Rays Toward A Global Extinction Crisis, Nicholas K. Dulvy, Nathan Pacoureau, Cassandra L. Rigby, Riley A. Pollom, Rima W. Jabado, David A. Ebert, Brittany Finucci, Caroline M. Pollock, Jessica Cheok, Danielle H. Derrick, Katelyn B. Herman, C. Samantha Sherman, Wade J. Vanderwright, Julia M. Lawson, Rachel H.L. Walls, John K. Carlson, Patricia Charvet, Kinattumkara K. Bineesh, Daniel Fernando, Gina M. Ralph, Jay H. Matsushiba, Craig Hilton-Taylor, Sonja V. Fordham, Colin A. Simpfendorfer

Biological Sciences Faculty Publications

The scale and drivers of marine biodiversity loss are being revealed by the International Union for Conservation of Nature (IUCN) Red List assessment process. We present the first global reassessment of 1,199 species in Class Chondrichthyes-sharks, rays, and chimeras. The first global assessment (in 2014) concluded that one-quarter (24%) of species were threatened. Now, 391 (32.6%) species are threatened with extinction. When this percentage of threat is applied to Data Deficient species, more than one-third (37.5%) of chondrichthyans are estimated to be threatened, with much of this change resulting from new information. Three species are Critically Endangered (Possibly Extinct), representing …


Simplifying The Development Of Portable, Scalable, And Reproducible Workflows, Stephen Piccolo, Zachary E. Ence, Elizabeth C. Anderson, Jeffrey T. Chang, Andrea H. Bild Oct 2021

Simplifying The Development Of Portable, Scalable, And Reproducible Workflows, Stephen Piccolo, Zachary E. Ence, Elizabeth C. Anderson, Jeffrey T. Chang, Andrea H. Bild

Faculty Publications

Command-line software plays a critical role in biology research. However, processes for installing and executing software differ widely. The Common Workflow Language (CWL) is a community standard that addresses this problem. Using CWL, tool developers can formally describe a tool’s inputs, outputs, and other execution details. CWL documents can include instructions for executing tools inside software containers. Accordingly, CWL tools are portable—they can be executed on diverse computers—including personal workstations, high-performance clusters, or the cloud. CWL also supports workflows, which describe dependencies among tools and using outputs from one tool as inputs to others. To date, CWL has been used …


The Coastal Monitor: Vol. 7 No. 3, John Tanacredi Ph.D. Oct 2021

The Coastal Monitor: Vol. 7 No. 3, John Tanacredi Ph.D.

The Coastal Monitor

Where has all the pollution gone? No hazardous toxic algal blooms? Incredible summer estuarine water quality! No fish kills. Banner years in recreational fisheries for Stripers and Blues and incredible numbers of Humpback whales! No going to restaurants, so greater at home use of water and septic systems! Since March 2020, drier years than most, thus reducing runoff with fertilizers and organic pesticides, resulting in reduced water contamination. So maybe, just maybe, this COVID- 19 period which has altered our collective lives forever, has also resulted in a grand experiment on what has been hiding in plain sight? That is, …


Assessing The Dispersal And Recruitment Of Stocked Walleye Fry In A Northern Minnesota Chain Of Lakes, Joseph W. Amundson Oct 2021

Assessing The Dispersal And Recruitment Of Stocked Walleye Fry In A Northern Minnesota Chain Of Lakes, Joseph W. Amundson

Biology Graduate Theses

As part of Minnesota's Walleye Sander vitreus egg take practice, 10% of eggs taken for hatchery purposes are stocked back into the donor lake. For Andrusia, part of the Cass Lake Chain of Lakes (Chain), this practice can result in elevated fry densities (mean: 17,000 fry/littoral hectare). However, if fry are able to disperse and use all available littoral hectares throughout the Chain, fry densities would be intermediate to typical stocking densities for Minnesota lakes (1,200 - 2,400 fry/littoral hectare). In 2016-2018, 3-3.5 million fry were mass-marked by immersion in oxytetracycline (OTC) prior to stocking into Andrusia to allow differentiation …


College Of Natural Sciences Newsletter, October 2021, College Of Natural Sciences Oct 2021

College Of Natural Sciences Newsletter, October 2021, College Of Natural Sciences

College of Natural Sciences Newsletters and Reports

Volume 2, Issue 10

Page 1 Dean's Message
Page 2 Awards and Recognition; Introducing Natural Sciences Student Ambassadors
Page 3 Media Coverage of CNS
Page 4 Spooky Science Outreach
Page 5 The BIG Event Outreach
Page 6 Geography Club Road Trip
Page 7 Aamlid Family Anatomy Lab
Page 8 Hobo Day Recap
Page 10 Open PRAIRIE Data; Grants Awarded in CNS


The Red Palm Weevil In The Uae: Morphological Diversity And Rnai-Mediated Gene Silencing Of Two Cuticle-Related Genes, Safa Hashem Mohammed Musaed Oct 2021

The Red Palm Weevil In The Uae: Morphological Diversity And Rnai-Mediated Gene Silencing Of Two Cuticle-Related Genes, Safa Hashem Mohammed Musaed

Theses

Red Palm Weevil (RPW), Rhynchophorus ferrugineus (Coleoptera: Curculionidae), threatens palm trees worldwide. A better understanding of this insect can help with designing an adequate management strategy. This study aimed to a better understand the morphological diversity of RPW and examined RNAi-mediated gene silencing of two cuticle-related genes, vestigial (vg) gene, and Laccase (Lac2) gene by injecting the last larval stage with double-stranded RNA (dsRNA). For the morphological diversity study, adults of RPW were collected and classified by their prothoracic spots. Additional morphological characters were measured such as Pronotum Length (PL), Pronotum Width (PW), Elytra Length (EL), Elytra Width (EW), and …