Biology Commons^™

Ice Storm Damage To Virginia Coastal Plain Forests During The Christmas 1998 Ice Storm, Peter Elstner, Stewart Ware

Virginia Journal of Science

On December 23-25, 1998, a major ice storm struck southeastern Virginia. The storm-deposited glaze ice felled trees and limbs, causing a power outage and highway blockage. Between February and April, 1999, we recorded occurrence, severity, and type of damage to trees over 2.5 cm dbh in nine mostly gently sloping plots in Matoaka Woods at the College of William and Mary. Frequency and severity of damage varied with species and with size of trees. Canopy damage occurred in 75% of large Fagus grandifolia trees, but in only 6% of small Sassafras albidum stems. As a group, small (2.5 to 15 …

Sustainable Tourism Plan For The Houtman Abrolhos Islands, Fisheries Western Australia

Fisheries Management Papers

The Abrolhos coral reefs are the southernmost living reefs in the Indian Ocean. They have a unique assemblage of tropical species of fish, reef corals and other invertebrates, which live in close association with temperate species, particularly of algae, and species endemic to Western Australia. The Abrolhos is the centre of the fishery for the commercially valuable western rock lobster (Panulirus cygnus). Terrestrial flora and fauna of the Abrolhos are relict and provide important reference areas on ecological interactions. Virtually all the islands of the Abrolhos archipelago have sea bird nesting and breeding areas, and populations of some species of …

A New Species Of Extinct Oryzomyine Rodent From The Quaternary Of Curaçao, Netherlands Antilles, Donald A. Mcfarlane, Adolphe O. Debrot

WM Keck Science Faculty Papers

An extinct new species of Oryzomyine rodent, known since 1936 from the cave of Grot van Hato on the island of Curaçao, is described from abundant new specimens collected from owl-pellet deposits at three localities on the island.

Effects Of Collection, Transport, And Redeployment Methods On Natural Mortality Of Rangia Cuneata (Mactridae) Used In Biomonitoring Studies, Eugene G. Maurakis, David V. Grimes, William C. Gretes

Virginia Journal of Science

Sporadic, high mortality in test populations of wedge clams (Rangia cuneata) has limited the potential for using this otherwise desirable test organism in biomonitoring studies. To determine whether high mortality was due to ontogenic or experimental variables, a two-phased study was conducted. In phase I, mortality of collected and re-deployed wedge clams, subjected to varying transport conditions, was determined at 7, 14, 21 and 60 days re-deployment. The use of three transport times (1, 2, 3 hr.), two vehicle conditions (open, closed) and three transport treatments (open, closed, iced containers) yielded 18 test groups. Individual test group mortalities …

Intrinsically Bent Dna In The Promoter Regions Of The Yeast Gal1–10 And Gal80 Genes, Ralph C. Bash, Jeff Vargason, P. Shing Ho, D. Lohr

Faculty Publications - Department of Biological & Molecular Science

Circular permutation analysis has detected fairly strong sites of intrinsic DNA bending on the promoter regions of the yeast GAL1–10 and GAL80 genes. These bends lie in functionally suggestive locations. On the promoter of the GAL1–10 structural genes, strong bends bracket nucleosome B, which lies between the UASG and the GAL1 TATA. These intrinsic bends could help position nucleosome B. Nucleosome B plus two other promoter nucleosomes protect the TATA and start site elements in the inactive state of expression but are completely disrupted (removed) when GAL1–10 expression is induced. The strongest intrinsic bend (;70°) lies at the downstream edge …

Hydrologic Events And Water Quality In The Pigeon River, Ottawa County, Michigan, Neil W. Macdonald, Richard R. Rediske, Jonathan E. Van Denend

Peer Reviewed Publications

The Pigeon River drains a 16,765-ha agricultural watershed in western Ottawa County, Michigan and discharges into south-central Lake Michigan. Extensive areas of wetlands in the upper watershed were drained in the 1920s, causing significantly altered hydrology characteristics by flashy discharges during storms and periods of snowmelt. We studied stream chemistry and hydrology for a four-year period between September, 1996, and October, 2000, to determine water quality status, to estimate annual nutrient exports, and to evaluate the effects of different seasonal flow types. Results of our study confirmed that the upper reaches of the Pigeon River experience chronically degraded water quality, …

Classification And Identification Of Pfiesteria And Pfiesteria-Like Species, Karen Steidinger, Jan Landsberg, R. William Richardson, Earnest Truby, Barbara Blakesley, Paula Scott, Patricia Tester, Torstein Tengs, Patrice Mason, Stever Morton, David Seaborn, Wayne Litaker, Kimberly Reece, David Oldach, Leonard Haas, Gerardo Vasta

Biological Sciences Faculty Publications

Dinoflagellates can be classified both botanically and zoologically; however, they are typically put in the botanical division Pyrrhophyta. As a group they appear most related to the protistan ciliates and apicomplexans at the ultrastructure level. Within the Pyrrhophyta are both unarmored and armored forms of the dominant, motile flagellated stage. Unarmored dinoflagellates do not have thecal or wall plates arranged in specific series, whereas armored species have plates that vary in thickness but are specific in number and arrangement. In armored dinoflagellates, the plate pattern and tabulation is a diagnostic character at the family, subfamily, and even genus levels. In …

Computer Aided Learning Environment For Biology, Pek Hin Foong

Student Works (2000-2009)

Traditional learning on biology requires of memory rather than understanding. As better educational technology emerges, we are able to utilise the computer as an important tool in learning. Many educational and pedagogical issues, particularly in the field or Artificial intelligence are looked at to help consider a good design for a computer learning tool. Existing computer aided learning materials are also studied to pinpoint their better features and incorporate them into the learning tool. A learning tool is proposed for effective learning in relating facts, rules, text and graphics. This system will have a Study Section containing notes that incorporates …

Yield-Per-Recruit Analysis For Black Drum, Pogonias Cromis, Along The East Coast Of The United States And Management Strategies For Chesapeake Bay, Cynthia M. Jones, Brian K. Wells

OES Faculty Publications

Black drum, Pogonias cromis along the U.S. East Coast is subject to commercial and recreational harvest. However, prior to this study no modeling had been undertaken to examine the potential for overfishing in the Chesapeake Bay region. We present evidence from yield-per-recruit models that growth overfishing of black drum is unlikely under current fishing practices in this region. Particular attention was given to fishing practices in the Chesapeake Bay region where old, large fish predominate in the commercial and recreational catches (mean age=26 years: mean total length=108.4 cm; mean weight 22.1 kg). Yield-per-recruit model results showed that growth overfishing was …

The Aquaculture Of Non-Endemic Species In Western Australia Silver Perch (Bidyanus Bidyanus), Tina Thorne, H. G. Brayford

Fisheries Management Papers

The development of commercial aquaculture of a non-endemic species such as silver perch raises a number of important issues relating to translocation. These include the potential of the introduced species to: impact on genetic diversity; introduce disease; and impact on - the natural environment; and - the biodiversity of native species. This Management Paper has been drafted in liaison with the Department of Environmental Protection in accordance with the principles of rish assessment, taking into account the above issues. It is considered that the implementation of this policy will protect the natural environment and native fish species. It will also …

High Efficiency Separation Of Microbial Aggregates Using Capillary Electrophoresis, J. M. Schneiderheinze, Daniel W. Armstrong, G. Schulte, David J. Westenberg

Chemistry Faculty Research & Creative Works

Recent advances in the technique of capillary electrophoresis have demonstrated fast, highly efficient separation of mixtures of intact microbes. This paper describes the application of this technique for the separation of microbial aggregates of Micrococcus luteus, Saccharomyces cerevisiae, or Alcaligenes faecalis. The aggregates of these microbes were resolved into several highly efficient peaks with analysis times under 10 min and efficiencies approaching 1 000 000 plates m-1 in some cases. A reproducible relationship was found between the electrophoretic mobility and the aggregation number or size of the cluster under a given set of experimental conditions. Often, cellular aggregation was reversible …

Fisheries Research Report No. 119 - Final Report, Frdc Project 95/037: The Biology And Stock Assessment Of The Tropical Sardine, Sardinella Lemuru, Off The Mid-West Coast Of Western Australia, Daniel J. Gaughan, Ronald W. Mitchell

Fisheries Research Reports

Exploratory fishing for the tropical sardine, Sardinella lemuru, offshore of Geraldton on the midwest coast of Western Australia in the early 1990s, led to the establishment of a developmental purse seine fishery in this region. This fishery showed potential for substantial expansion, but as there was no information on S. lemuru in WA there was a need to undertake research during the developmental period. The biology and fishery for S. lemuru in WA were therefore investigated over a three year period between July 1995 and June 1998 with the aim of providing stock assessment advice.

The biology of S. lemuru …

Fisheries Research Report No. 121 - Synopsis Of The Biology And Exploitation Of The Blue Swimmer Crab, Portunus Pelagicus Linnaeus, In Western Australia, Mervi I. Kangas

Fisheries Research Reports

This report summarises the existing studies which have provided information on the biology, population characteristics and exploitation of the blue swimmer crab, Portunus pelagicus, in Western Australia. Gaps in the existing knowledge are also highlighted to assist in targeting new research initiatives. Portunus pelagicus matures at about one year and in temperate regions females mate only once a year. This is because mature crabs moult only once a year. It appears from studies to date that Western Australian crabs are one genetic population. This is being further investigated. Migration of the blue swimmer crab occurs between estuaries and the ocean …

The Age Of The Woolly Rhino From Dream Cave, Derbyshire, Uk, Donald A. Mcfarlane, Joyce Lundberg, Derek C. Ford

WM Keck Science Faculty Papers

The Dream Cave woolly rhinoceros, Coelodonta antiquitatis, is a "classic" specimen of a "cold-stage" fossil fauna from central England. The find was illustrated and described by Dean William Buckland in his seminal tome Reliquiae Diluvianae (1823) during the first half of the 19th century, and made a significant contribution to the development of Buckland's views on the origin of extinct and extirpated fossil vertebrates. The report presents the first, albeit indirect, radiometric dates on the specimen, and argues that the animal fell into the cave just before 37,000 years BP, during the middle of Marine Isotope Stage 3 Interstadial (41 …

Characterization Of Chimeric Lipopolysaccharides From Escherichia Coli Strain Jm109 Transformed With Lipooligosaccharide Synthesis Genes (Lsg) From Haemophilus Influenzae, Nancy J. Phillips, T. J. Miller, J. J. Engstrom, William Melaugh, R. Mclaughlin, M A. Apicella, B W. Gibson

Chemistry Faculty Publications

Previously, we reported the expression of chimeric lipopolysaccharides (LPS) in Escherichia coli strain JM109 (a K-12 strain) transformed with plasmids containing Haemophilus influenzae lipooligosaccharide synthesis genes (lsg) (Abu Kwaik, Y., McLaughlin, R. E., Apicella, M. A., and Spinola, S. M. (1991) Mol. Microbiol. 5, 2475–2480). In this current study, we have analyzed the O-deacylated LPS and free oligosaccharides from three transformants (designated pGEMLOS-4, pGEMLOS- 5, and pGEMLOS-7) by matrix-assisted laser desorption ionization, electrospray ionization, and tandem mass spectrometry techniques, along with composition and linkage analyses. These data show that the chimeric LPS consist of the complete E. coli LPS core …

Reflections Of A Naturalist: Papers Honoring Professor Eugene D. Fleharty, Jerry R. Choate

Fort Hays Studies Series

Science and academia tend to be dominated by persons who are utterly brilliant, or who are proficient at self-promotion, or who are willing to sacrifice their personal lives and work twice as hard as their peers, or who exhibit some combination of these attributes. Occasionally, however, someone comes along who is just "ordinary" compared with academic overachievers but whose accomplishments far exceed reasonable expectations. Eugene D. Fleharty is such a person. Although he is extraordinarily bright, few would describe him as utterly brilliant. He has never been inclined to "toot his own horn" or otherwise promote himself. And, although he …

New Specimens Of Late Quaternary Extinct Mammals From Caves In Sanchez Ramirez Province, Dominican Republic, Donald A. Mcfarlane, Abel Vale, Keith Christenson, Joyce Lundberg, Gabriel Atilles, Stein Erik Lauritzen

WM Keck Science Faculty Papers

During the late Quaternary, the island of Hispaniola supported one of the most diverse mammalian faunas in the West Indies. Much of this diversity was lost to extinction in the past 100,000 years, but the timing of these events is poorly known. Here we report the paleontological findings of a multidisciplinary investigation of caves in the central Dominican Republic. These findings include new 'last occurrence' dates for the rodents Isolobodon portoricensis and Brotomys cf. voratus that take these genera to the dawn of the historic era; a first record of a last-interglacial sloth, and the first report of the upper …

Cytotoxic Mab From Rheumatic Carditis Recognizes Heart Valves And Laminin, Jeffrey E. Galvin, Mark E. Hemric, Kent Ward, Madeleine W. Cunningham

Faculty Publications and Presentations

Anti-streptococcal antibodies cross-reactive with N-acetyl-bD-glucosamine (GlcNAc) and myosin are present in the sera of patients with rheumatic fever (RF). However, their role in tissue injury is not clear. In this study, we show that anti-GlcNAc/anti-myosin mAb 3.B6 from a rheumatic carditis patient was cytotoxic for human endothelial cell lines and reacted with human valvular endothelium and underlying basement membrane. Reactivity of mAb 3.B6 with the valve was inhibited by human cardiac myosin > laminin > GlcNAc. The mAb 3.B6 epitopes were localized in fragments of human cardiac myosin, including heavy meromyosin (HMM), the S1 subfragment, and two light meromyosin (LMM) peptides containing …

A Determination Of Parentage, Mating System, And Genetic Diversity In A Captive Population Of The Straw-Colored Fruit Bat (Eidolon Helvum), Jan Zinck

Dissertations and Theses

Small populations tend to lose genetic variability. The magnitude of this loss is influenced by the number of founding individuals, the genetic diversity of the founders, and the species mating system. Genetic variability is the basis of adaptive evolution, and the loss of genetic variability may have harmful effects on development, growth, and survival. Therefore, a primary management goal for small, captive populations is the retention of genetic variability. Of considerable importance to conservation biology is the determination of parentage, from which mating, genetic, and demographic information can be derived. Microsatellites provide a robust molecular evolutionary tool for the study …

Theories Of The Origin And Early Evolution Of Life, David A. Dewitt

Faculty Publications and Presentations

After the theory of spontaneous generation was discredited, only religious explanations were offered to explain the origin of life. Alexander Oparin (1894-1980), an atheist, suggested that natural chemical reactions produced biological molecules that came together to form the first living thing. Later, Stanley Miller tested this hypothesis and produced chemical "building blocks" but not life itself. In spite of much progress, there is still no clear consensus on how life originated on Earth. Some scientists are even looking to outer space for the origin of life.

Late Quaternary Fossil Mammals And Last Occurrence Dates From Caves At Barahona, Puerto Rico, Donald A. Mcfarlane

WM Keck Science Faculty Papers

Puerto Rico supported at least five genera of endemic terrestrial mammals in the late Quaternary, all of which are extinct. Whether these animals died out in the late Pleistocene, the mid-Holocene, or in post-Columbian time has not been established. This paper is the first attempt at radiometrically dating the 'last occurrences' of these taxa, together with the first unambiguous descriptions of localities reported by previous workers. Last occurrence dates for Nesophontes, Elasmodontomys and Heteropsomys are shown to be mid-Holocene and overlap with Amerindian occupation of the island. Acratocnus is known only from the late Pleistocene. No Puerto Rican taxon has …

Black-Tailed Deer Habitat Changes In A Portion Of The Mount St. Helens Blast Zone, Ronald W. Davis

All-Inclusive List of Electronic Theses and Dissertations

This study utilized Landsat TM imagery to evaluate black-tailed deer habitat changes in a portion of the Mount Saint Helens blast zone in western Washington. The purpose of this study was to compare the post eruption vegetation changes with population trends for black tailed deer. The 55,000-acre study area comprises a Washington Department of Game Management unit (GMU 524) located to the northnorthwest of Mount St. Helens. Approximately 80% ofthe mostly forested study area was affected by lateral blast from the 1980 eruption. The area is predominantly private land managed for timber production but a small portion falls within the …

Three-Dimensional Reconstructions Of Tadpole Chondrocrania From Historical Sections, Gary Radice, Mary K. Boggiano, Mark Desantis, Peter Larson, Joseph Oppong, Matthew Smetanick, Todd Stevens, James Tripp, Rebecca Weber, Michael Kerckhove, Rafael De Sá

Virginia Journal of Science

Reconstructing three dimensional structures (3DR) from histological sections has always been difficult but is becoming more accessible with the assistance of digital imaging. We sought to assemble a low cost system using readily available hardware and software to generate 3DR for a study of tadpole chondrocrania. We found that a combination of RGB can1era, stereomicro­scope, and Apple Macintosh PowerPC computers running NIH Image, Object Image, Rotater, and SURFdriver software provided acceptable reconstruc­tions. These are limited in quality primarily by the distortions arising from histological protocols rather than hardware or software.

Avian Communities Of Large Reclaimed Mine Grasslands In Southwestern Indiana, Travis L. Devault

All-Inclusive List of Electronic Theses and Dissertations

Many bird species native to the tallgrnss prairie region oCthc central U.S. have declined drastically since European settlement due lo the destruction and alteration of grassland habitat. In this study, the breeding bird communities or 21 large reclaimed surface coal mine grasslands in a seven-county area or southwestern Indiana were examined with respect to species and guild composition, density, variation among sites, and grassland size. In addition, the vegetation characteristics of nine large mine grasslands were described and related to the presence and abundance or eight grasslund bird species common to the study areas. The immense size of mine grasslands …

The Translocation Of Barramundi. A Discussion Paper., Makaira Pty Ltd

Fisheries Management Papers

There is recognisable economic benefit to the translocation of barramundi, but there is also a need to ensure the translocation will not adversely impact upon the genetic diversity, introduce disease or impact on the natural environment and biodiversity. This discussion paper has been prepared to provide information to assist in the assessment of the possible impact of translocation of barramundi into and within Western Australia, for the purposed of recreational stock enhancement, aquaculture development and domestic stocking. In contemplating the translocation any aquatic species, signigicant economic and social benefits must be balanced with biological and environmental risks; that is, the …

Characterization And Identification Of A Sludge-Associated Bacterial Isolate, Abiodun O. Adibi, Derrell Mcpherson, Minna Laine

Virginia Journal of Science

During a metal speciation study, an unusually high retention of lead ·was observed when lead solution was percolated through a column packed with sludge compost obtained from Hampton Roads Sanitation department. A bacterium was isolated from the sludge compost and identified as Bacillus sphaericus using electron microscopy, whole cell fatty acid analysis (Midi System) and Biolog GP Microplate. The isolate grows in broth and agar media containing up to 800 µM lead. Lead accumulation study using atomic absorption spectrophotometer indicates that the isolate adsorbs lead. Lead adsorption is pH dependent. The isolate contains a plasmid of approximately 40 -50 kbp …

Method For Identification Of Origins Of Replication And Genes Regulated By Dnaa In Bacteria, Olga G. Troyanskaya

Honors Theses

The study is focused on developing computer programs to identify origin of DNA replication based on analysis of total bacterial genomes, scoring regions for number of DnaA binding sites, AT content, DNA adenine methylase boxes, and integration host factors binding sites. The programs were tested on cyanobacterium Synechocystis, and several potential origins were identified. However, no one definite region could be located. Currently, software is being developed to analyze common motifs around the origins of all bacteria with known origins. Genes whose transcription could be regulated by DnaA were identified by searching for DnaA boxes preceding promoter regions.

Cyclopropane Fatty Acid Expression In Plants, Katherine M. Schmid

Scholarship and Professional Work - LAS

Pants [sic] are transformed with a bacterial cyclopropane fatty acid synthase gene to produce lipids containing cyclopropane fatty acids. Using this technology dihydrosterculate is produced in oilseed crops such as rape.

Reproduction And Health Of A Beaver (Castor Canadensis) Population In Prince William County, Virginia, Timothy R. Brophy, Carl H. Ernst

Faculty Publications and Presentations

Reproduction and health were studied in a beaver (Castor canadensis) population from Prince William County, Virginia during 1998. Copulating beavers were observed in Quantico Creek at Prince William Forest Park on 22 January. Seven females from Quantico Marine Base were trapped between January and May, sacrificed, and dissected. Those reproductively active weighed over 39 pounds (17.7 kg) and were trapped before 1 March. Mean litter size based on counts of corpora lutea was 4.80 young (3-7); however, litter size based on the number of embryos present was only 2.75 (1-3), comparable to most others reported in the literature. Five of …

Field Sampling And Necropsy Examination Of Fish, A. S. Kane, A. Baya, R. Reimschuessel, K. M. St. Pé, C. A. Poukish, C. P. Driscoll

Virginia Journal of Science

This paper presents an overview of observational and fish sampling tech­niques for investigating fish lesions, morbidity and mortality. These sam­pling techniques and investigations are much like detective work and require attention to detail, common sense, technical proficiency and experience. To solve the mystery of a fish kill, the investigator must use available evidence and clues to piece together a series of events that often have long since passed. The cause of these field events may be chemical, biological or physical; more often, it is some combination of these. An initial categorization approach may be used to reduce the great number …