Biology Commons^™

Protection Against Experimental Autoimmune Myocarditis Is Mediated By Interleukin-10-Producing T Cells That Are Controlled By Dendritic Cells, Ya Li, Janet S. Heuser, Stanley D. Kosanke, Mark E. Hemric, Madeleine W. Cunningham

Faculty Publications and Presentations

Experimental autoimmune myocarditis (EAM) can be induced in the Lewis rat by cardiac myosin or its cryptic S2-16 peptide epitope (amino acids1052 to 1076). To investigate cellular mechanisms and the role of antigen-presenting cells in regulation of myocarditis, we induced protection against EAM in Lewis rats by administration of S2-16 peptide in incomplete Freund’s adjuvant (IFA). Protection to EAM was associated with activation of S2-16-reactive splenocytes secreting high levels of interleukin (IL)-10 and reduced levels of interferon- and IL-2. Adoptive transfer of S2-16:IFA-induced splenocytes producing IL-10 suppressed myocarditis induction in syngeneic recipients, suggesting their regulatory cell nature. However, exposure of …

Evaluation Of Glycine Max Mrna Clusters, Ronald L. Frank, Fikret Erçal

Biological Sciences Faculty Research & Creative Works

Background: Clustering the ESTs from a large dataset representing a single species is a convenient starting point for a number of investigations into gene discovery, genome evolution, expression patterns, and alternatively spliced transcripts. Several methods have been developed to accomplish this, the most widely available being UniGene, a public domain collection of gene-oriented clusters for over 45 different species created and maintained by NCBI. The goal is for each cluster to represent a unique gene, but currently it is not known how closely the overall results represent that reality. UniGene's build procedure begins with initial mRNA clusters before joining ESTs. …

Fisheries Occasional Publication No.21 - Allocation Of The Western Rock Lobster Resource Between User Groups, Department Of Fisheries Western Australia

Fisheries Occasional Publications

In this submission to IFAAC, the Department will outline what it believes are the most practical and cost effective ways of managing a system of allocation amongst the commercial, customary and recreational sectors. This recommendation recognises that cost efficiencies and outcomes will be greatly affected by the type of allocation model that is chosen. Consequently the Department recommends that the allocation system should be based on a single allocation to each sector that is available over the entire fishery.

The Department does not recommend having any finer scale zoning of the allocations, or the creation of exclusive recreational fishing areas. …

Alternative Assay Techniques For Protein Kinase A, Justin Brink

Honors Capstones

Capstone submitted as a graduation requirement for the BSU Honors Program.

The 19th Century Excavation Of Kent's Cavern, England, Donald A. Mcfarlane, Joyce Lundberg

WM Keck Science Faculty Papers

Between 1858 and 1880, William Pengelly developed revolutionary new techniques for the archeological and paleontological excavation of cave deposits. His work at Brixham Cave and Kent’s Cavern, England, yielded tens of thousands of specimens from the mid-Pleistocene to the Holocene, settled the intellectual debate over the co-existence of humans and extinct mammals, and accumulated an unparalleled resource for continued study. Although the Brixham Cave work was thoroughly summarized in print, Pengelly never published the plans of his much more thorough and extensive excavations at Kent’s Cavern. Here we present a reconstructed plan of the Pengelly excavations that we hope will …

Two Purified Domains Of Telomerase Reverse Transcriptase Reconstitute Sequence-Specific Interactions With Rna, C. M. O'Connor, Cary K. Lai, Kathleen Collins

Biology Faculty Publications

Telomerase reverse transcriptase (TERT) and telomerase RNA (TER) function together to create a uniquely specialized polymerase. Here we have described for the first time domains of bacterially expressed Tetrahymena TERT that interacted directly with TER in the absence of assembly chaperones. We used quantitative binding assays to define TER sequence requirements for recognition by the high affinity RNA binding domain and an independent N-terminal RNA interaction domain. The TERT RNA binding domain and N-terminal RNA interaction domain had distinct, nonoverlapping requirements for TER sequence and structure that together accounted for all of the sites of TER contact inferred for full-length …

Preservation Of Eumelanin Hair Pigmentation In Proopiomelanocortin-Deficient Mice On A Nonagouti (A/A) Genetic Background, Andrzej Slominski, Przemyslaw M. Plonka, Alexander Pisarchik, James L. Smart, Virgine Tolle, Jacobo Wortsman, Malcolm J. Low

Faculty Publications - Department of Biological & Molecular Science

No abstract provided.

Demonstration Of Toxicity To Fish And To Mammalian Cells By Pfiesteria Species: Comparison Of Assay Methods And Strains, Joann M. Burkholder, Andrew S. Gordon, Peter D. Moeller, J. Mac Law, Kathryn J. Coyne, Alan J. Lewitus, John S. Ramsdell, Harold G. Marshall, Nora J. Deamer, S. Craig Cary, Jason W. Kempton, Steven L. Morton, Parke A. Rublee

Biological Sciences Faculty Publications

Toxicity and its detection in the dinoflagellate fish predators Pfiesteria piscicida and Pfiesteria shumwayae depend on the strain and the use of reliable assays. Two assays, standardized fish bioassays (SFBs) with juvenile fish and fish microassays (FMAs) with larval fish, were compared for their utility to detect toxic Pfiesteria. The comparison included strains with confirmed toxicity, negative controls (noninducible Pfiesteria strains and a related nontoxic cryptoperidiniopsoid dinoflagellate), and P. shumwayae strain CCMP2089, which previously had been reported as nontoxic. SFBs, standardized by using toxic Pfiesteria (coupled with tests confirming Pfiesteria toxin) and conditions conducive to toxicity expression, reliably detected …

Rich Mesic Forests: Edaphic And Physiographic Drivers Of Community Variation In Western Massachusetts, J. Bellemare, G. Motzkin, D. R. Foster

Biological Sciences: Faculty Publications

Rich Mesic Forest, a Northeastern variant of the species-rich Mixed Mesophytic Forest association of eastern North America, is an Acer saccharum-dominated forest type typically associated with calcareous bedrock and nutrient-rich, mull soils. Rich Mesic Forest (RMF) is a priority for conservation in the Northeast due to its limited areal extent, high plant species richness, and numerous rare taxa, yet the community characteristics and environmental correlates of this forest type are incompletely understood. This study undertook a quantitative classification of RMF of the northeastern edge of the Berkshire Plateau in western Massachusetts. Cluster analysis of data from ten sites identified two …

Punishment And Rehabilitation Views Of Social Work Majors And Non-Social Work Students: An Exploratory Study, Eric G. Lambert, Sudershan Pasupuleti, Reva I. Allen

Journal of the South Carolina Academy of Science

No abstract provided.

The Subsystems Approach To Genome Annotation And Its Use In The Project To Annotate 1000 Genomes, Ross Overbeek, Tadhg Begley, Ralph M. Butler, Jomuna V. Choudhuri, Han-Yu Chuang, Matthew Cohoon, Valérie De Crécy-Lagard, Naryttza Diaz, Terry Disz, Robert Edwards, Michael Fonstein, Ed D. Frank, Svetlana Gerdes, Elizabeth M. Glass, Alexander Goesmann, Andrew Hanson, Dirk Iwata-Reuyl, Roy Jensen, Neema Jamshidi, Lutz Krause, Michael Kubal, Niels Larsen, Burkhard Linke, Alice C. Mchardy, Folker Meyer, Heiko Neuweger, Gary Olsen, Robert Olson, Andrei Osterman, Vasiliy Portnoy, Gordon D. Pusch, Dmitry A. Rodionov, Christian Rückert, Jason Steiner, Rick Stevens, Ines Thiele, Olga Vassieva, Yuzhen Ye, Olga Zagnitko, Veronika Vonstein

Chemistry Faculty Publications and Presentations

The release of the 1000^th complete microbial genome will occur in the next two to three years. In anticipation of this milestone, the Fellowship for Interpretation of Genomes (FIG) launched the Project to Annotate 1000 Genomes. The project is built around the principle that the key to improved accuracy in high through put annotation technology is to have experts annotate single subsystems over the complete collection of genomes, rather than having an annotation expert attempt to annotate all of the genes in a single genome. Using the subsystems approach, all of the genes implementing the subsystem are analyzed by …

Knowing When To Draw The Line: Designing More Informative Ecological Experiments, Kathryn L. Cottingham, Jay T. Lennon, Bryan L. Brown

Dartmouth Scholarship

Linear regression and analysis of variance (ANOVA) are two of the most widely used statistical techniques in ecology. Regression quantitatively describes the relationship between a response variable and one or more continuous independent variables, while ANOVA determines whether a response variable differs among discrete values of the independent variable(s). Designing experiments with discrete factors is straightforward because ANOVA is the only option, but what is the best way to design experiments involving continuous factors? Should ecologists prefer experiments with few treatments and many replicates analyzed with ANOVA, or experiments with many treatments and few replicates per treatment analyzed with regression? …

Design And Cloning Of A Hammerhead Ribozyme Targeted Tohiv-1p7fmrna, Katherine L. Anderson, William H. Jackson

Journal of the South Carolina Academy of Science

No abstract provided.

The Architecture Of A Proteomic Network In The Yeast, Emad Ramadan, Christopher Osgood, Alex Pothern

Biological Sciences Faculty Publications

We describe an approach to clustering the yeast protein-protein inter-action network in order to identify functional modules, groups of proteins forming multi-protein complexes accomplishing various functions in the cell. We have developed a clustering method that accounts for the small-world nature of the network. The algorithm makes use of the concept of k-cores in a graph, and employs recursive spectral clustering to compute the functional modules. The computed clusters are annotated using their protein memberships into known multi-protein complexes in the yeast. We also dissect the protein interaction network into a global subnetwork of hub proteins (connected to several clusters), …

Comparison Of Phytoplankton And Autotrophic Picoplankton Populations Over A 24-Hour Period From A Pond's Surface And Subsurface Waters, Harold G. Marshall, Lubomira Burchardt, Elżbieta Wilk-Woźniak

Biological Sciences Faculty Publications

Phytoplankton abundance and composition relationships within the surface layer changed over short time periods (4-6 hours) in comparison with more constant associations at 2 cm below the surface. Both strata had a diverse algal flora (>50 taxa), but no distinct neuston assemblage characterized the surface layer algal composition over the 24-hour study. The similarity between the two strata indicated the floral composition of the surface layer came from the water column algae below the surface. Chlorophytes, diatoms, cyanobacteria, and cryptophytes represented the most abundant algal categories for both strata with a total mean phytoplankton abundance of 3,566 cells ml …

Zooplankton Communities Of Selected Stations Of Lake Victoria, Rk Waya, Ja Mwambungu

Tanzania Journal of Science

Species composition and diversity of zooplankton communities of Lake Victoria (Tanzanian waters) were studied. In the shallow waters zooplankton were collected between March and April, and between August and December 2000. In deeper waters zooplankton were collected in August 2001. Overall, 20 genera and 29 species of zooplankton were observed in the shallow waters whereas 12 genera and 20 species of zooplankton were encountered in the deeper waters. The highest abundance was recorded in the deeper waters (2,481,633-2). In the shallow waters the cyclopoid copepods dominated the zooplankton community, the common species being Thermocyclops emini and Thermocyclops neglectus. In the …

The Diversity Of Benthic Mollusks Of Lake Victoria And Lake Burigi, Ja Mwambungu

Tanzania Journal of Science

Molluscan diversity, abundance and distribution in sediments of Lake Victoria and its satellite lake, Lake Burigi, were investigated. The survey was carried out in January and February 2002 for Lake Victoria and in March and April 2002 for Lake Burigi. Ten genera were recorded from four zones of Lake Victoria while only three genera were recorded from Lake Burigi. In Lake Victoria the most diverse zone was Mara zone. The mollusca communities comprised of Bellamya, Gabbiella, Melanoides, Bulinus, Biomphalaria and Cleopatra (Gastropoda) and Corbicula, Eupera (Byssanodonta), Caelatura and Sphaerium (Bivalvia). Lake Burigi comprised of Melanoides, Bulinus and Biomphalaria. Melanoides tuberculata …

Phytoplankton And Nutrients Studies In Magu Bay, Speke Gulf, Lake Victoria, (Tanzania) Following The 2001 Principal Rain Season, Jf Machiwa, C Lugomela, Ann Muzuka

Tanzania Journal of Science

Phytoplankton abundance and species composition in relation to some physico-chemical parameters were studied in Magu Bay, Lake Victoria, in May 2001. Investigations on the influence of Simiyu River on the biological and physical characteristics of the Bay were carried out. Surface and bottom currents flowed in the northeast direction close to the river mouth but were completely reversed after about 1.5 km from the river mouth. Most suspended particles brought in by the river were deposited within 0.5 km after entering the lake. Nutrient concentrations were generally high towards the eastern part of the bay as compared to the rest …

Species Composition And Biomass Estimates Of Zooplankton In Some Water Bodies Within Lake Victoria Basin, Rk Waya, Ai Chande

Tanzania Journal of Science

Zooplankton communities were identified and their abundance and biomass estimated in some selected satellite lakes and Mara River in Lake Victoria basin, during wet and dry seasons in 2002. Samples were collected from eight water bodies. Three stations were sampled from each water body using a plankton net (40 µm mesh size) hauled vertically through the water column. The zooplankton community comprised mainly crustacea and rotifers. The results show that the number of species collected during the wet and dry seasons was 31 species and 28 species, respectively. The Shannon wiener diversity indices were not significantly different (Mann-Whitney U Test …

Spatial Distribution Of Parasites Along The Gut Of The Catfish Clarias Gariepinus (Burchell, 1822) (Clariidae) From The Mwanza Gulf, Lake Victoria, G Nkwengulila, C Mwita

Tanzania Journal of Science

The diversity, abundance and spatial distribution of parasites in the alimentary tract of Clarias gariepinus from the Mwanza Gulf were investigated. Ten parasite species were recovered; the Trematoda, Cestoda and Nematoda were each represented by three species while Dolops ranarum was the only crustacean recovered from the posterior part of the buccal cavity. Parasites diversity was highest in the small intestine and the stomach. Although the gut community of C. gariepinus studied was rich in intensity and individual numbers of helminth species, helminth infection in most sections of the gut comprised of monospecific infection and thus cohabiting interspecific interaction was …

Diurnal Feeding Patterns And Food Habits Of Lates Niloticus In The Speke Gulf, Lake Victoria, Ha Mhitu, Ai Chande

Tanzania Journal of Science

Twenty four hour sampling sessions were conducted to investigate the feeding patterns and food habits of Lates niloticus in the Speke Gulf, Lake Victoria during January/February, July/August and December 2002. Feeding activity was more pronounced during daytime mostly in morning hours than during the night. The most dominant food item was haplochromines. There was a significant difference in the occurrence of food items in the gut of L. niloticus with time. However, there was no significant difference in the occurrence of food items in the habitat at different times. The study concludes with a recommendation for fishermen to set their …

Fish Species Composition, Size Structure And Distribution In Non-Trawlable Areas Of Lake Victoria (Tanzania) With Emphasis On Mwanza Gulf And Mori Bay, Jdr Bayona, B Msuku, S Mzighani

Tanzania Journal of Science

Fish diversity studies in littoral non-trawlable areas of Lake Victoria (Tanzania) were undertaken during six systematic surveys (November 2000 to December 2002). Information on fish species composition, size structure as well as spatial and temporal distribution was generated from gill-netting, beach-seining and electric fishing. Lates niloticus, indicated a declining trend in composition from 78.9% in March 2001 to 5.1% in July 2002. The species, however, increased to 40.6 % in catch composition as of December 2002, exhibiting somewhat inverse trends in distribution with Oreochromis niloticus and haplochromines in the lake fisheries. The surveys identified over 50 species for which 42 …

Total Mercury Concentration In Common Fish Species Of Lake Victoria, Tanzania, J F Machiwa

Tanzania Journal of Science

Total mercury (THg) concentration was analysed in muscles of common fish species of Lake Victoria in the eastern and southern parts of the lake using cold vapour Atomic Absorption Spectrophotometric technique. Mercury concentration in all fish species was generally lower than the WHO maximum allowable concentration (500 ng/g ww) in edible fish parts. The maximum concentration of THg in Lates niloticus samples was 396 ng/g ww. Samples of Oreochromis niloticus had THg concentration ranging between <0.01 and 419 ng/g ww. Clarias gariepinus had THg concentration ranging between 1.2 and 239.1 ng/g ww. All less common fish species, such as, Labeo victirianus, Synodontis victoriae, Oreochromis leucostictus, Schilbe intermedius, Brycinus jacksonii, B. sadleri, Tilapia rendalii, Tilapia zilii and Haplochromines had THg concentration below the WHO maximum allowable limit (500 ng/g ww). The study showed that Hg from small-scale gold mining activities in Lake Victoria basin is not transported to the lake by fluvial discharge. Other possible pathways of Hg input to Lake Victoria are discussed.

Studies On The Fisheries And Biology Of Oreochromis Urolepsis (Pisces: Cichilidae) In The Mtera Resorvoir (Tanzania), F M M Chale

Tanzania Journal of Science

The fisheries and some aspects of the biology of Oreochromis urolepis of the Mtera Reservoir (Tanzania) were examined in August 2002. It was observed that there was no proper management of the reservoir's fisheries. Beach seine fishing dominated the fishery. Nearly all the O. urolepis caught by this type of gear were juveniles. The number of fish caught by the beach seines was very high, some times up to 4,000 fish per haul. The beach seines also destroy the spawning sites for the tilapias. Mean total lengths of O. urolepis caught in gillnets of legal mesh sizes (ranging from 88 …

Parasites Of Clarias Gariepinus (Burchell, 1822) (Pisces: Clariidae) From The Mwanza Gulf, Lake Victoria, C Mwita, G Nkwengulila

Tanzania Journal of Science

Seventeen species of parasites were recovered from 1071 Clarias gariepinus examined from the Mwanza Gulf of Lake Victoria. The parasite fauna comprised of four ectoparasites, a Monogenea, Hirudinea, crustacean and a Digenea; and fourteen endoparasites, five nematodes, five trematodes and three cestodes. Twelve parasite species were adults and five were larval forms. Ten were identified to species, six to genus and one to family level. Many species recorded are common to C. gariepinus, a few, e.g. Tylodelphys species is a first record in fish of Africa, thus represents a new host record. Spinitectus petterae is reported for the first time …

Use Of Cre Recombinase And Sb Transposase To Engineer Transgenic Zebrafish, Jie Dong

All-Inclusive List of Electronic Theses and Dissertations

Current transgenesis has become easier for certain species due to the recent development of exogenous non site-specific recombination systems such as Sleeping Beauty (SB) transposon, and site-specific recombination systems such as Cre-loxP. Such recombination systems overcame two major problems caused by conventional transgenesis, position effect and copy number effect. SB is a reconstructed fish transposon that has shown to efficiently insert transgenes into heterologous mammalian chromosomes. The Cre-loxP system is frequently used to delete a given sequence from the genome or to target the integration of foreign DNA. The use of both systems in combination, however, has not yet been …

Correction [To Maurakis And Grimes Article, V. 54, #3&4]

Virginia Journal of Science

This page is a correction to an article by Eugene G. Maurakis and David V. Grimes: Predicting Fish Species Diversity in Lotic Freshwaters of Greece, published in Virginia Journal of Science Volume 54, numbers 3 and 4. Amended table 2a owed to a printing corruption.

Subterranean Loss And Gain Of Water In Mountain Lake, Virginia: A Hydrologic Model, Martin Jansons, Bruce C. Parker, Jacob E. Waller

Virginia Journal of Science

Mountain Lake, Virginia is a small, unique, oligotrophic, subalpine ecosystem in the southern Appalachians. Previous studies have disclosed that this lake has manifested periodic prolonged low water levels during the several thousand years of its existence. The most recent low water level occurred during the drought years of 1999-2002. Measurements of lake level, precipitation, and other meteorological data including calculated evapotranspiration in the lake basin from 2/19/02 to 8/31/03 have enabled estimation of net subterranean water losses presumably through cracks between Clinch sandstone boulders and/or the recently discovered deep hole at the northwest end of Mountain Lake. These net losses …

Visualizing Early Frog Development With Motion-Sensitive 3-D Optical Coherence Microscopy, Richard C. Haskell, Mary E. Williams, Daniel C. Petersen, Barbara M. Hoeling, Andrew J. Schile, J. D. Pennington, M. G. Seetin, J. M. Castelaz, Scott E. Fraser, Cyrus Papan, Hongwu Ren, Johannes F. De Boer, Zhongping Chen

All HMC Faculty Publications and Research

A motion-sensitive en-face-scanning 3-D optical coherence microscope (OCM) has been designed and constructed to study critical events in the early development of plants and animals. We describe the OCM instrument and present time-lapse movies of frog gastrulation, an early developmental event in which three distinct tissue layers are established that later give rise to all major organ systems. OCM images constructed with fringe-amplitude data show the mesendoderm migrating up along the blastocoel roof, thus forming the inner two tissue layers. Motion-sigma data, measuring the random motion of scatterers, is used to construct complementary images that indicate the presence of Brownian …

Immunogold Labeling To Enhance Contrast In Optical Coherence Microscopy Of Tissue Engineered Corneal Constructs, Chris B. Raub, Elizabeth J. Orwin, Richard C. Haskell

All HMC Faculty Publications and Research

Our lab has used an optical coherence microscope (OCM) to assess both the structure of tissue-engineered corneal constructs and their transparency. Currently, we are not able to resolve cells versus collagen matrix material in the images produced. We would like to distinguish cells in order to determine if they are viable while growing in culture and also if they are significantly contributing to the light scattering in the tissue. In order to do this, we are currently investigating the use of immunogold labeling. Gold nanoparticles are high scatterers and can create contrast in images. We have conjugated gold nanoparticles to …