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Articles 811 - 840 of 2216
Full-Text Articles in Biology
The Effects Of Timing And Long-Term Estrogen Deficiency On Cardiac Fibrosis Following Chronic Sympathetic Stimulation, Samuel Wisecarver
The Effects Of Timing And Long-Term Estrogen Deficiency On Cardiac Fibrosis Following Chronic Sympathetic Stimulation, Samuel Wisecarver
Undergraduate Honors Theses
Evidence in animal studies show that estrogen in women is cardioprotective, but discrepancies remain between clinical and animal studies regarding progression of cardiovascular disease after menopause. Cardiac fibrosis, the build-up of type I and III collagen fibers, is a staple of cardiac remodeling. The goal of this study was to examine the effects of long-term estrogen loss in the heart; this study includes two time points (1-month and 5-month) post-ovariectomy. Mice were randomly selected for 4 treatment groups: SHAM, Ovariectomy (OVX), Isoproterenol (ISO) (a β-adrenergic agonist used to mimic chronic sympathetic stimulation) and OVX+ISO. Mice were infused with either Saline …
Biases And Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens, Merve Dede
Biases And Blind-Spots In Genome-Wide Crispr-Cas9 Knockout Screens, Merve Dede
Dissertations and Theses (Open Access)
Adaptation of the bacterial CRISPR-Cas9 system to mammalian cells revolutionized the field of functional genomics, enabling genome-scale genetic perturbations to study essential genes, whose loss of function results in a severe fitness defect. There are two types of essential genes in a cell. Core essential genes are absolutely required for growth and proliferation in every cell type. On the other hand, context-dependent essential genes become essential in an environmental or genetic context. The concept of context-dependent gene essentiality is particularly important in cancer, since killing cancer cells selectively without harming surrounding healthy tissue remains a major challenge. The toxicity of …
Understanding An Inflammatory Pathway In Diabetic Retinopathy, Reanna Raye Rodriguez
Understanding An Inflammatory Pathway In Diabetic Retinopathy, Reanna Raye Rodriguez
Theses and Dissertations
Diabetic Retinopathy (DR) is the leading cause of blindness in the U.S. However, not much is known of its molecular pathway and how it attributes to increases in inflammatory response in the eye. One avenue we will investigate is the transforming growth factor beta (TGFB) signaling pathway and its effect of vascular endothelial growth factor (VEGF) secretion and cell viability. VEGF is the hallmark that exacerbates DR progression in prolonged diabetes. Some major concern that have arisen are the underlying effects of oxidants and antioxidants in elevating VEGF secretion in diabetes. In attempt to learn more, we evaluated how an …
The Effect Of Egcg-P Containing Formulations On Bacillus Spp., Sabrina A. Lopez
The Effect Of Egcg-P Containing Formulations On Bacillus Spp., Sabrina A. Lopez
Seton Hall University Dissertations and Theses (ETDs)
The Gram-positive Bacillus species is known for their spore-forming abilities. These spores are highly resistant to a multitude of factors such as heat, UV radiation, and chemical solvents, which can be attributed to the location of its DNA, and other components that make up the spore as a whole. Bacillus spp. can be detrimental to the food & beverage industry and the medical field due to their production of enterotoxins, which can manifest as bacterial infections. Antibiotic-resistant bacteria towards current treatments are of major concern, and thus the need for alternative therapeutic agents is urgent. The beneficial health properties of …
Molecular Drivers Of Bladder Cancer Motility, Bryan Wehrenberg
Molecular Drivers Of Bladder Cancer Motility, Bryan Wehrenberg
Dissertations and Theses (Open Access)
Bladder cancer (BC) progression is measured by the degree of tumor cell invasion into the bladder wall and dissemination to distant sites. The study of BC cell motility will both enable development of anti-invasion therapeutics to limit progression of early-stage disease and improve our understanding of the metastatic process which drives patient mortality in BC. BCs display a great deal of intertumoral heterogeneity, and can be divided into basal and luminal subtypes, which are biologically and clinically distinct entities. Here, I examine the invasion phenotypes of BC as a function of both subtype and epithelial to mesenchymal transition (EMT) status. …
Investigating Bmp7 Expression In Glioblastoma Multiforme, Yajaira Janett Macias
Investigating Bmp7 Expression In Glioblastoma Multiforme, Yajaira Janett Macias
Theses and Dissertations
The PI3K/AKT/mTOR pathway regulates important cell processes such as growth, survival, motility, inflammation, proliferation, and apoptosis. In Glioblastoma multiforme (GBM) the PI3K/AKT/mTOR pathway is aberrant as it is almost always active. This results in the deregulation of downstream molecules and ultimately leads to cancer progression and maintenance in GBM tumors. In this study, we used RNA-sequencing to identify differentially expressed genes (DEGs) in U87MG GBM cells treated with NVP-BEZ235, a dual inhibitory drug targeting PI3K and mTOR. A total of 7,803 differentially expressed genes were identified via RNA-seq. GEPIA2 online tool was used to assess differential gene expression …
Effectiveness Of Visual Representations In Undergraduate Human Anatomy And Physiology I & Ii, Mary Agnes Mestayer
Effectiveness Of Visual Representations In Undergraduate Human Anatomy And Physiology I & Ii, Mary Agnes Mestayer
Honors Theses
The objective of this project was to explore intersections between student preferences and student performance on anatomical visual representations in Human Anatomy and Physiology I and II. Visual representations are a critical resource for the formation of relationships between function and structure furthermore; students interpret these representations uniquely based on specific factors (learning objective, prior knowledge, the diagram studied, etc.). Phase I of this project gathered undergraduate responses to ten Likert-style questions on their opinions on diagrams and their use in the A&P classroom. Phase II of this project presented participants with twelve manipulated diagrams sourced from three diagram with …
Perivascular Adipose Tissue In Relation To Diet, Thermogenesis And Cardiovascular Health, Ginger Paquette, Caitlin Stieber, Ashely Soucy, Benjamin Tero, Lucy Liaw
Perivascular Adipose Tissue In Relation To Diet, Thermogenesis And Cardiovascular Health, Ginger Paquette, Caitlin Stieber, Ashely Soucy, Benjamin Tero, Lucy Liaw
Thinking Matters Student Research Symposium
Adipose tissue is a diverse and crucial component to vascular health due to its role in energy storage and heat production. The primary function of white adipose tissue (WAT) is energy storage while the function of mitochondria-rich brown adipose tissue (BAT) is heat production. Perivascular adipose tissue (PVAT), which surrounds blood vessels, contains both WAT and BAT adipocytes. Dietary calorie restriction is associated with increased lifespan with decreased adiposity. Increased prevalence of WAT-like PVAT, due to high-fat diets and obesity, leads to increased metabolic disfunction and cardiovascular-disease. We used a calorie-restriction model in C57BL6/J mice to test the hypothesis that …
Changes In Gene Sequence That Cause Discordances Involving Disease In Monozygotic Twins, Kacie Jacques
Changes In Gene Sequence That Cause Discordances Involving Disease In Monozygotic Twins, Kacie Jacques
Thinking Matters Student Research Symposium
Identical twins or, monozygotic twins, occur when the egg in a mother is fertilized, but one zygote divides into two separate embryos. Monozygotic twins are considered to be genetically identical, but most twins often display discordances, some involving diseases or disorders. Some of these differences are due to environmental factors. For example, one twin may have diabetes due to diet. Recent literature has shown that some differences in monozygotic twins may be due to changes in gene sequence, as opposed to random, environmental or epigenetic factors. Identical twins from pedigrees with familial disease often show discordances. When looking at schizophrenia …
A Novel Application Of Galleria Mellonella Larvae To Study Pathogenesis Of Rickettsia Rickettsii Infection, Jacob M. Jones
A Novel Application Of Galleria Mellonella Larvae To Study Pathogenesis Of Rickettsia Rickettsii Infection, Jacob M. Jones
Honors College Theses
Rickettsia are obligate intracellular tick-borne bacteria that cause acute undifferentiated febrile illness accompanied by headache, malaise, myalgias, and disseminated endothelial infection. Immune responses to Rickettsia and pathogenesis of rickettsioses are not fully understood. Current work with Rickettsia is limited to vertebrate models that are strictly regulated and have low throughput. Since the innate immune system of vertebrates retains striking similarities with the immune response in insects, invertebrates are being used to study host responses to various pathogens. The purpose of this study was to evaluate if larvae of the greater wax moth Galleria mellonella (GML) can be used as an …
How Gata3 Affects Neuronal Survival In The Inner Ear, Hiba Ahmad
How Gata3 Affects Neuronal Survival In The Inner Ear, Hiba Ahmad
Honors Theses
Broadly speaking, Gata3 is a transcription factor involved in neurosensory specification and hair cell differentiation. Previous studies have shown that Gata3 null mutants do not develop neurons, so Gata3 appears to be necessary for neuronal survival. To better understand the role of Gata3 in spiral ganglion neurons of the inner ear, a Neurod1-cre model was used to conditionally knock out Gata3 at E9-E9.5, the timepoint when neuroblasts first begin to delaminate from the otic placode. The mutants used in this study were Nd1-cre: Gata3 f/f mutants. In situ was performed to determine gene expression within the cochlea, and immunohistochemistry was …
Use Of Ultrasounds And Cystotomies In Diagnosing And Resolving Bladder Stones, Kirsten Cline
Use Of Ultrasounds And Cystotomies In Diagnosing And Resolving Bladder Stones, Kirsten Cline
Biology Presentations
The use of ultrasounds are the most safe and effective way to detect bladder stones. Bladder stones are easy to detect with acoustic shadowing, a white bright twinkle effect, and heaviness. Cystotomies are one of the most common procedures to solve bladder stones with one of the lowest mortality and morbidity rates. Kiara, a one year old feline presented with UTI symptoms and was diagnosed with bladder stones. An ultrasound was used and a cystotomy was performed.
Crayfish And Climate Change: How The Growing Acidity Of Freshwater Lakes, Streams, And Ponds Negatively Impact The Nervous System Of Crayfish, Lauren Irmen
Honors Theses
The nervous system of crayfish, a freshwater crustacean, is largely regulated by the flow of calcium (Ca2+) ions in a cell across a concentration gradient that produces electrical impulses and controls movement (Krause et al., 1998). Crayfish obtain Ca2+ from their external aquatic environment. Therefore, rapid changes in the concentration of Ca2+ in freshwater environments may cause negative impacts to the neural system of crayfish and subsequently the freshwater food chain. These impact of altered Ca2+ levels may create hyperactive or hypoactive movement due to over-, under-stimulation, respectfully in the skeletal muscle of the crayfish, …
The Interconnectivity Of Parkinson's Disease And Type Two Diabetes Mellitus, Erica Olfson
The Interconnectivity Of Parkinson's Disease And Type Two Diabetes Mellitus, Erica Olfson
Honors Scholars Collaborative Projects
This thesis is about the biochemical connection between Parkinson's disease and Type II Diabetes Mellitus.
Expression And Purification Of Hpep Inhibitor Peptide, Olivia Plaza
Expression And Purification Of Hpep Inhibitor Peptide, Olivia Plaza
Undergraduate Research Conference
The enzyme human prolyl peptidase, or hPEP, has been linked to many processes within the brain, including breaking down neural peptides. A bovine milk protein, a-s1-casein (see above), has been found to inhibit hPEP in colon cancer cells. A 68 amino acid long section (bolded) of a-s1- casein containing the inhibitor region was cloned. The casein fragment expression was optimized for expression of the peptide. The purpose for optimizing expression is to have much of it on hand to utilize in further studies on its inhibitory properties on hPEP. By using this fragment of casein, one can study the structure …
The Giant Axolotl Genome Uncovers The Evolution, Scaling, And Transcriptional Control Of Complex Gene Loci, Siegfried Schloissnig, Akane Kawaguchi, Sergej Nowoshilow, Francisco Falcon, Leo Otsuki, Pietro Tardivo, Nataliya Timoshevskaya, Melissa C. Keinath, Jeramiah J. Smith, S. Randal Voss, Elly M. Tanaka
The Giant Axolotl Genome Uncovers The Evolution, Scaling, And Transcriptional Control Of Complex Gene Loci, Siegfried Schloissnig, Akane Kawaguchi, Sergej Nowoshilow, Francisco Falcon, Leo Otsuki, Pietro Tardivo, Nataliya Timoshevskaya, Melissa C. Keinath, Jeramiah J. Smith, S. Randal Voss, Elly M. Tanaka
Biology Faculty Publications
Vertebrates harbor recognizably orthologous gene complements but vary 100-fold in genome size. How chromosomal organization scales with genome expansion is unclear, and how acute changes in gene regulation, as during axolotl limb regeneration, occur in the context of a vast genome has remained a riddle. Here, we describe the chromosome-scale assembly of the giant, 32 Gb axolotl genome. Hi-C contact data revealed the scaling properties of interphase and mitotic chromosome organization. Analysis of the assembly yielded understanding of the evolution of large, syntenic multigene clusters, including the Major Histocompatibility Complex (MHC) and the functional regulatory landscape of the Fibroblast Growth …
Ceramic Studio Assistant, Allison Jones, Adam Yungbluth
Ceramic Studio Assistant, Allison Jones, Adam Yungbluth
2021 Celebration of Student Scholarship - Oral Presentations
The research project will focus on learning the various activities involved in running a college level Ceramics Studio. Activities will include clay mixing for Ceramics 1 courses, mixing studio glazes & slips, firing bisque, reduction, soda, & wood kiln, and other activities to improve the Ceramics Studio.
The Art Of Preservation, Sinclaire Dorsey, Melissa Yungbluth
The Art Of Preservation, Sinclaire Dorsey, Melissa Yungbluth
2021 Celebration of Student Scholarship - Oral Presentations
Proper preservation of creative works is imperative to sustaining documentation for future generations’ education. Exhibition designers, such as the students awarded with the undergraduate research fellowship for the Golding-Yang gallery, must be mindful of the environmental factors that impact creative works. For instance, light sensitivity of the materials can be overwhelmed by ultraviolet and infrared radiation produced by the sun. Atmospheric humidity, when not regulated, will destroy irreplaceable artwork that could provide future historians a better understanding of modern culture. Extreme temperatures are devastating to the preservation of artistic works. The regulation of these environmental factors will provide future generations …
Low Stress Handling Techniques In Shelter Animals And The Overall Effects On Physiological Parameters, Ashley Swim, Courtney Smart, Amy Staton
Low Stress Handling Techniques In Shelter Animals And The Overall Effects On Physiological Parameters, Ashley Swim, Courtney Smart, Amy Staton
2021 Celebration of Student Scholarship - Oral Presentations
Animal shelters tend to be a very stressful environment for dogs as there are many new sounds, smells, and animals. This stress is not only bad for their psychological health and can lead to behavioral issues, but it can also have an impact on their physical health. Stress impacts multiple systems of the body, resulting in increased heart rate, blood pressure, respiratory rate, temperature and even blood glucose levels in dogs. The impact stress has on these systems can lead to a compromised immune system and increases the probabilities of the dog becoming ill. However, by using Fear Free methods, …
Adverse Experiences, Depression, And Parenting Sensitivity., Lauren Oldiges, Savannah Gillis, Tiffany Hicks, Shari Kidwell
Adverse Experiences, Depression, And Parenting Sensitivity., Lauren Oldiges, Savannah Gillis, Tiffany Hicks, Shari Kidwell
2021 Celebration of Student Scholarship - Oral Presentations
Parental sensitivity is a key determinant of attachment security and child well-being. Yet many parents in our region face challenges to parenting, such as increased exposure to adverse experiences and depression. Both cumulative exposure to adverse experiences and depression symptoms have been associated with increased risk to parenting (Hays-Grudo & Wilson, 2020). The present study explores connections between parents’ sensitivity/synchrony scores on the Toddler CARE-Index (TCI: Crittenden, 2007), Adverse Childhood Experiences (ACEs) amongst parents, and parental depression. As part of a larger longitudinal study of Eastern Kentucky families, 35 children (mean age 4.5 years) and their parents participated in the …
French Textbook Illustrations, Abigail Hall, Nicholas Anderson, Karen Taylor
French Textbook Illustrations, Abigail Hall, Nicholas Anderson, Karen Taylor
2021 Celebration of Student Scholarship - Oral Presentations
This project has been an update of an introductory French textbook in order to make the work more cohesive illustratively. Each week we were given a drawing task to go with a chapter or vocabulary assignment to replace, revamp or develop new art work of greater value than the preexisting illustrations.
Relation Among Potential Predictors Of Drug Abuse Risk In Rats, Emma Brock
Relation Among Potential Predictors Of Drug Abuse Risk In Rats, Emma Brock
2021 Celebration of Student Scholarship - Oral Presentations
Animal models have been used to investigate behavioral processes and mechanisms that underlie addiction. A variety of measures have been developed for rats that are modest predictors of drug abuse risk. The purpose of this pilot study was to begin to assess whether several established and potential measures of drug abuse vulnerability were correlated. The subjects were eight adult male Wistar rats. The rats were run on procedures that yielded measures of anxiety, sensation seeking, short term responsive to acutely administered drug, and sensitivity to conditioned stimuli. These measures were correlated with a potential new measure of drug abuse vulnerability—withdrawal …
Improving Patient Outcomes With High Nurse To Patient Ratios: A Quality Improvement Study, Alexis Bradshaw, Morgan Minton, Brenna Murphy, Abigail Ross, Hannah Dorton, Carson Jones, Cierra Connelly, Haley Nyderek
Improving Patient Outcomes With High Nurse To Patient Ratios: A Quality Improvement Study, Alexis Bradshaw, Morgan Minton, Brenna Murphy, Abigail Ross, Hannah Dorton, Carson Jones, Cierra Connelly, Haley Nyderek
2021 Celebration of Student Scholarship - Oral Presentations
According to the United States Registered Nurse Workforce Report Card and Shortage Forecast: A Revisit published in the May/June 2018 issue of the American Journal of Medical Quality, a shortage of registered nurses is projected to spread across the country between 2016 and 2030. Surveyed nurses see the shortage in the future as a catalyst for increasing stress on nurses (98%), lowering patient care quality (93%) and causing nurses to leave the profession (93%) (American Association of Colleges of Nursing, 2021). A thorough literature review was conducted, and 40 studies were reviewed. Common themes that emerged include: high nurse to …
The Impact Of Power Yoga Training On Baseball Throwing Velocity, John Bakke
The Impact Of Power Yoga Training On Baseball Throwing Velocity, John Bakke
2021 Celebration of Student Scholarship - Oral Presentations
The purpose of this study was to examine the impact of yoga on baseball pitching velocity, and the factors that affect throwing velocity such as power, flexibility, speed, and mobility. Thirteen volunteer collegiate baseball pitchers were pre-tested on their power, flexibility, speed, mobility, and throwing velocity. The intervention included a 10-week yoga training, with the goal of three sessions per week. Participants were then post-tested under the same conditions. Analysis included dependent t-tests for each of the fitness assessments, with alpha set at .05 a priori. The group was unable to reach the goal of three yoga sessions per week, …
Increasing Student Engagement In Introductory Physics Hybrid Classes, Breanna Epperson
Increasing Student Engagement In Introductory Physics Hybrid Classes, Breanna Epperson
2021 Celebration of Student Scholarship - Oral Presentations
The demand for online instruction is on the rise. In a classroom setting, research shows that student interactions are important for overcoming common misconceptions, but it is challenging to achieve the same level of engagement in an online setting. To try to improve online interactions in a hybrid physics class at Morehead State University, the Fall 2020 online class was required to post a total of three times about a closed, multiple choice, conceptual physics question in each assignment while following certain criteria. In the Spring of 2021, the discussion format was kept the same, but was moved to a …
Genetically Screening For Gain Of Function Mutations In Telomerase Rna, Jose Childers
Genetically Screening For Gain Of Function Mutations In Telomerase Rna, Jose Childers
2021 Celebration of Student Scholarship - Oral Presentations
Telomeres are DNA structures located at the ends of linear chromosomes. They function, in part, to allow DNA ends to be copied before cells divide. Since the standard DNA replication machinery can’t fully copy telomeres, most eukaryotic cells require the vital ribonucleoprotein enzyme telomerase. Telomerase is composed of a TERT (telomerase reverse transcriptase protein) and telomerase RNA. As we age, telomeres shorten since most human cells do not express telomerase. If telomeres grow too short, it triggers a cell-cycle arrest known as senescence, which can ultimately result in cell death. On the other hand, >85% of human cancers show over-expression …
A Portable Analyzer For Rapid And Sensitive Protein Detection By Ac Electrokinetics Capacitive Sensing, Allie Skaggs
A Portable Analyzer For Rapid And Sensitive Protein Detection By Ac Electrokinetics Capacitive Sensing, Allie Skaggs
2021 Celebration of Student Scholarship - Oral Presentations
Capacitive bioparticle detection is a promising method for point-of-care diagnosis. There are a wide range of applications for such a device: diagnostics, pharmacology, and medical science. The end goal of this project is to develop a portable capacitive sensing platform for rapid and sensitive bioparticles detection. When bioparticles bind to receptors located on the surface of the electrode there would be a change in the interfacial capacitance, which indicate the presence of the bioparticle. The ADuCM355 – a precision analog microcontroller with a chemical sensor interface – is being used to perform biased electrochemical impedance spectroscopy (EIS). An AC potential …
Preservation Of Morehead State History, Jacob Bush
Preservation Of Morehead State History, Jacob Bush
2021 Celebration of Student Scholarship - Oral Presentations
This project is being undertaken in an effort to preserve the buildings and history of Morehead State University. It is the goal of this project to make additions to the historic district of MSU by adding qualifying buildings to the National Register of Historic Places. By adding buildings to the Register, they would be recognized on the federal level as historic sites and receive support for their preservation. The Register seeks to protect sites of importance through many ways including the gifting of funding to ensure preservation. The goals of this project will be achieved using historical texts from our …
Analytical Method Development For The Analysis Of Biomass Degradation Products, Calista Dean
Analytical Method Development For The Analysis Of Biomass Degradation Products, Calista Dean
2021 Celebration of Student Scholarship - Oral Presentations
The goal of this project is to study the kinetics of hydrothermal liquefaction of biomass. Biomass contains stored chemical energy that can be converted to renewable liquid and gaseous fuels through various processes, but an in-depth understanding of the kinetics of these processes is important in order for them to be feasible on a large scale. Since biomass degradation products depend on the type of biomass used as well as the reaction conditions, this task can be quite complicated. To minimize complications in our initial studies, we will use D-glucose as the starting material. To better understand the degradation pathway’s …
Comparing Growth Of Mutant Strains Of Acinetobacter Baumannii To Wild Type After Treatment With Mitomycin C, Kaylee Compton, Deborah Cook
Comparing Growth Of Mutant Strains Of Acinetobacter Baumannii To Wild Type After Treatment With Mitomycin C, Kaylee Compton, Deborah Cook
2021 Celebration of Student Scholarship - Oral Presentations
In Acinetocbacter baumannii bacteria, an error-prone DNA damage repair response is induced by the self-cleavage of the UmuDAb protein. Previous experiments indicating that a ddrR mutant becomes growth sensitive after DNA damage suggested that ddrR is also involved in this process. To explore this relationship, eight mutant strains of A. baumannii were made with different combinations of mutations in either ddrR and/or the adjacent gene, A1S_3662. Our experiment compared the growth of wild type cells to strains containing various mutations: DC4 (no gene mutations, just an antibiotic resistance marker, Kanr), DC5 (stop codon mutation in ddrR), and DC6 (both the …