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Full-Text Articles in Biophysics
Mössbauer Spectroscopic Studies Of Hemoglobin And Its Isolated Subunits, G. R. Hoy, D. C. Cook, R. L. Berger, F. K. Friedman
Mössbauer Spectroscopic Studies Of Hemoglobin And Its Isolated Subunits, G. R. Hoy, D. C. Cook, R. L. Berger, F. K. Friedman
Physics Faculty Publications
Samples of 90% enriched 57Fe hemoglobin and its isolated subunits have been prepared. Mössbauer spectroscopic measurements have been made on three such samples. Sample one contained contributions of oxyhemoglobin, deoxyhemoglobin, and carbonmonoxyhemoglobin. This sample was studied from a temperature of 90 K down to 230 mK. Measurements were also made at 4.2 K using a small applied magnetic field of 1.0 T. In general, the measured quadrupole splittings and isomer shifts for each component agreed with previous measurements on single component samples in the literature, and thus demonstrated that chemically enriched hemoglobin has not been altered. The second and …
Concanavalin A-Nonbinding Enzymes Of Crotalus Scutulatus Scutulatus Venom, C. K. Childs, Michael W. Hinson, Dewey H. Sifford, Bob D. Johnson
Concanavalin A-Nonbinding Enzymes Of Crotalus Scutulatus Scutulatus Venom, C. K. Childs, Michael W. Hinson, Dewey H. Sifford, Bob D. Johnson
Journal of the Arkansas Academy of Science
Crotalus scutulatus scutulatus crude venom was separated into two fractions by Concanavalin A Sepharose 4B affinity chromatography. The Concanavalin A-nonbinding fraction (F-l) exhibited phosphomonoesterase (orthophosphoric monoester phosphohydrolase EC 3.1 .3.2), phosphodiesterase, 5 '-nucleotidase (5 '-ribonucleotide phosphohydrolase EC 3.1.3.5), phospholipase A (phosphatidate 2-acylhydrolase EC 3.1.1.4), hyaluronidase (hyaluronate glycanohydrolase EC 3.2.1.d), N-benzoyl-Larginine ethyl esterase, p-toluenesulfonyl-L-arginine methyl esterase, L-amino acid oxidase (L-amino acid: O2 oxidoreductase [deaminating] EC 1.4.3.2), and caseinolytic activities. Thrombin-like and NAD nucleosidase (5 '-ribonudeotide phosphohydrolase EC 3.1.3.5) activities were not observed. DEAE Sephadex A-50 ion exchange chromatography by two stage elution of F-l yielded several fractions having proteinase activities. Proteinase …
Plasma Corticosterone Levels In Cholesterol-Fed Cockerels Before And After A Twenty Minute Run, Stanley N. David, Clarene L. David
Plasma Corticosterone Levels In Cholesterol-Fed Cockerels Before And After A Twenty Minute Run, Stanley N. David, Clarene L. David
Journal of the Arkansas Academy of Science
Cockerels were exercised to observe the influence of physical activity on plasma corticosterone concentrations. The birds were maintained on a commercial mash or on an atherogenic diet. Plasmas were collected on the first day, fifteen days after the first collection and immediately after a 20 minute run on that fifteenth day. The plasma corticosterone levels as determined by radioimmunoassay showed extreme variations within collections. Hence, the data analysis indicated no significant changes of the Compound β in the blood of the cockerels due to diet, or exercise or the combination of both. The causes for the wide variation of plasma …