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Articles 391 - 392 of 392
Full-Text Articles in Biochemistry
Examining The Effects Of Netropsin On The Mobility Of Dna A-Tracts Using Capillary Electrophoresis, Jillian Miller
Examining The Effects Of Netropsin On The Mobility Of Dna A-Tracts Using Capillary Electrophoresis, Jillian Miller
Honors Program Theses
A-tracts are sequences of repeated adenine bases that cause a natural curvature of DNA. The biological function of A-tracts is not fully understood. Research into how A-tract sequences affect mobility in an electric field contributes more knowledge in this area. For this research project, the electrophoretic mobility of multiple double-stranded DNA (dsDNA) fragments of the same length, with varying shapes due to the amount and orientation of A-tracts along the helix axis, was studied using capillary electrophoresis under varying conditions with the small molecule netropsin. Previous semesters of research showed a change in mobility due to shape of three fragments …
Identification Of Fungi And Their Secondary Metabolites From Wind Cave, Kyle Biscoglia
Identification Of Fungi And Their Secondary Metabolites From Wind Cave, Kyle Biscoglia
Honors Program Theses
Eighteen fungal samples were isolated from samples of water collected in Wind Cave, South Dakota. Ten of the samples underwent chemical extraction, a growth inhibition test, and were analyzed using proton nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectroscopy. Structural analysis and identification of one secondary metabolite that demonstrated antimicrobial growth inhibition was performed. All eighteen of the fungal samples were able to be identified to the genus level using the ITS region of their DNA, however confident identification of the species level was more difficult to attain just looking at one portion of the sequence.