Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Physical Sciences and Mathematics (111)
- Chemistry (99)
- Molecular Biology (74)
- Medicine and Health Sciences (62)
- Cell and Developmental Biology (53)
-
- Biotechnology (49)
- Other Biochemistry, Biophysics, and Structural Biology (47)
- Biology (41)
- Genetics and Genomics (35)
- Biophysics (28)
- Structural Biology (26)
- Cell Biology (25)
- Microbiology (22)
- Bioinformatics (21)
- Physiology (21)
- Engineering (20)
- Cancer Biology (19)
- Chemicals and Drugs (15)
- Molecular Genetics (15)
- Pharmacology, Toxicology and Environmental Health (15)
- Diseases (14)
- Neuroscience and Neurobiology (14)
- Ecology and Evolutionary Biology (13)
- Genetics (13)
- Medical Sciences (13)
- Medical Specialties (13)
- Plant Sciences (10)
- Institution
-
- University of Nebraska - Lincoln (39)
- Chulalongkorn University (20)
- University of Kentucky (18)
- Old Dominion University (17)
- Utah State University (15)
-
- City University of New York (CUNY) (11)
- Aga Khan University (10)
- Technological University Dublin (10)
- University of Montana (10)
- West Virginia University (10)
- University at Albany, State University of New York (9)
- Loyola University Chicago (8)
- University of Missouri, St. Louis (8)
- Wayne State University (8)
- Louisiana State University (7)
- University of Louisville (7)
- Western University (7)
- East Tennessee State University (6)
- Northwestern College, Iowa (6)
- Purdue University (6)
- University of Nebraska Medical Center (6)
- Calvin University (5)
- Chapman University (5)
- Cleveland State University (5)
- The Texas Medical Center Library (5)
- University of Arkansas, Fayetteville (5)
- University of Denver (5)
- University of South Carolina (5)
- Virginia Commonwealth University (5)
- Augustana College (4)
- Keyword
-
- Biochemistry (8)
- Chemistry (8)
- Cancer (7)
- Killifish (6)
- RNA (6)
-
- Bioinformatics (5)
- Metabolomics (5)
- Chemistry and Biochemistry (4)
- Lipids (4)
- Mass spectrometry (4)
- Meiothermus ruber (4)
- ABC transporter (3)
- Algae (3)
- Annotation (3)
- Biological Sciences (3)
- Biosensors (3)
- Chemoresistance (3)
- DNA (3)
- E. coli (3)
- Escherichia coli (3)
- FRET (3)
- GENI-ACT (3)
- Genome (3)
- Messenger RNA (3)
- Microalgae (3)
- Nitrogenase (3)
- Oligomerization (3)
- Oxidative Stress (3)
- Prostate cancer (3)
- SAXS (3)
- Publication
-
- Department of Biochemistry: Faculty Publications (34)
- Electronic Theses and Dissertations (23)
- Chemistry and Biochemistry Faculty Publications (21)
- Chulalongkorn University Theses and Dissertations (Chula ETD) (20)
- Articles (10)
-
- Department of Biological & Biomedical Sciences (10)
- Theses and Dissertations (10)
- Dissertations, Theses, and Capstone Projects (9)
- Faculty & Staff Scholarship (9)
- Legacy Theses & Dissertations (2009 - 2024) (9)
- Chemistry: Faculty Publications and Other Works (8)
- Wayne State University Dissertations (8)
- Chemistry Faculty Publications (7)
- Chemistry & Biochemistry Faculty Works (6)
- Killifish Research Review (6)
- Theses & Dissertations (6)
- Dissertations and Theses (Open Access) (5)
- LSU Doctoral Dissertations (5)
- Theses and Dissertations--Chemistry (5)
- Biology, Chemistry, and Environmental Sciences Faculty Articles and Research (4)
- Dissertations (4)
- Faculty Publications (4)
- Graduate Theses and Dissertations (4)
- Loma Linda University Electronic Theses, Dissertations & Projects (4)
- Meiothermus ruber Genome Analysis Project (4)
- Summer Research (4)
- The Summer Undergraduate Research Fellowship (SURF) Symposium (4)
- USF Tampa Graduate Theses and Dissertations (4)
- Williams Honors College, Honors Research Projects (4)
- Biochemistry Publications (3)
- Publication Type
- File Type
Articles 391 - 399 of 399
Full-Text Articles in Biochemistry
Study Of Hiv-1 Reverse Transcriptase And Protease Inhibition By Mushroom Extracts, Chanin Sillapachaiyaporn
Study Of Hiv-1 Reverse Transcriptase And Protease Inhibition By Mushroom Extracts, Chanin Sillapachaiyaporn
Chulalongkorn University Theses and Dissertations (Chula ETD)
Human immunodeficiency virus type-1 (HIV-1) can cause acquired immunodeficiency syndrome (AIDS), a world serious public health problem. HIV-1 protease (HIV-1 PR) and reverse transcriptase (HIV-1 RT) are necessary target enzymes which have been used for antiretroviral drugs development. Herein, Lignosus rhinocerus (LR), Auricularia polytricha (AP) and Astraeus spp. (AH) crude extracts were screened for inhibitory activities on both enzymes. Crude hexane extract of AP (APH) exhibited significant inhibition on both HIV-1 PR and RT activities not only in an in vitro non-cell based but also cell based assays. In HIV-1 infected MOLT-4 cell model, APH significantly reduced capsid protein (p24) …
การพัฒนาแบบจำลองเซลล์ลำไส้ของมนุษย์บนกระดาษที่มีการดัดแปรพื้นผิวและต้นแบบไบโอเซนเซอร์ไร้สายสำหรับตรวจวัดการเจริญของจุลชีพก่อโรค, ปัณณวิชญ์ ถิรบวรกิจพิธาน
การพัฒนาแบบจำลองเซลล์ลำไส้ของมนุษย์บนกระดาษที่มีการดัดแปรพื้นผิวและต้นแบบไบโอเซนเซอร์ไร้สายสำหรับตรวจวัดการเจริญของจุลชีพก่อโรค, ปัณณวิชญ์ ถิรบวรกิจพิธาน
Chulalongkorn University Theses and Dissertations (Chula ETD)
วิทยานิพนธ์นี้เป็นการนำเสนองานวิจัย การพัฒนาวิธีการใหม่เพื่อควบคุมและเฝ้าระวังโรคติดเชื้อ โดยประกอบด้วยเนื้อหาสำคัญสองส่วน ได้แก่ (i) การพัฒนาแบบจำลองเซลล์ลำไส้ของมนุษย์ที่เพาะเลี้ยงบนกระดาษที่มีการดัดแปรพื้นผิว เพื่อใช้ประโยชน์ในการศึกษากลไกการบุกรุกของเชื้อจุลชีพก่อโรคในระบบทางเดินอาหาร และ (ii) การพัฒนาต้นแบบไบโอเซนเซอร์แบบไร้สายเพื่อการตรวจติดตามการเจริญของเชื้อจุลชีพก่อโรค โดยในส่วนแรก เซลล์ลำไส้ของมนุษย์ HT-29 จำนวน 200,000 เซลล์ ถูกเพาะเลี้ยงในบริเวณชอบน้ำของกระดาษกรอง Whatman No.1 โดยกระดาษถูกดัดแปรพื้นผิวด้วยสารแมทริกซ์นอกเซลล์ (Extracellular matrix: ECM) ชนิดต่าง ๆ ได้แก่ Matrigel, Collagen-1 และ Laminin พื้นที่เพาะเลี้ยงเซลล์มีขนาด 12.56 ตารางมิลลิเมตร ภายใต้สภาวะที่เหมาะสม เซลล์ HT-29 ที่ถูกเพาะเลี้ยงบนกระดาษ มีชีวิตรอดได้ถึง 28 วัน และแสดงลักษณะของเซลล์ที่เจริญเต็มที่ โดยมีการแสดงออกของวิลไล (villi) ที่ผิวเซลล์ด้านบน (apical) และมีการแสดงออกของโปรตีน ZO-1 ซึ่งแสดงถึงเซลล์มีการสร้างไทต์จังก์ชันเกิดขึ้น จากการใช้สารเชื่อมพันธะสำหรับเชื่อมพันธะ ECM แต่ละชนิด พบว่าเมื่อใช้สารเจนิพินในการเชื่อมพันธะ เซลล์มีชีวิตรอดทั้งหมด (100 -112.8%) ในขณะที่มีเพียง 1.1 - 67.3 % ของเซลล์รอดชีวิต หากใช้สารกลูตารัลดีไฮด์ในการเชื่อมพันธะ แต่การใช้สารเชื่อมพันธะทั้งสองชนิด ทำให้เซลล์ไม่สามารถแบ่งตัวมีรูปร่างแบบสามมิติได้ เมื่อสังเกตด้วย SEM เซลล์มีลักษณะแบน การดัดแปรพื้นผิวกระดาษด้วยสาร ECM ชนิด Matrigel ส่งผลให้เซลล์มีชีวิตรอดมากที่สุดและสาร ECM ชนิด Laminin ทำให้เซลล์เกิดการแปรสภาพ (cell differentiation) ได้ดีที่สุด งานวิจัยส่วนที่ 2 เป็นการพัฒนาไบโอเซนเซอร์ไร้สายสำหรับติดตามการเจริญของเชื้อจุลชีพก่อโรค ได้แก่ เชื้อ Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus gordonii และ Candida albicans พบว่าเชื้อดังกล่าวสามารถสร้างไบโอฟิล์มบนผิวของขั้วไฟฟ้าคาร์บอนอสัณฐาน (glassy carbon electrode) และสามารถให้ค่าศักย์ไฟฟ้าที่เป็นลบได้ …
The Interaction Between Ceramide-1-Phosphate And Group Iva Cytosolic Phospholipase A2 And Its Role In Wound Healing, Patrick Macknight
The Interaction Between Ceramide-1-Phosphate And Group Iva Cytosolic Phospholipase A2 And Its Role In Wound Healing, Patrick Macknight
Theses and Dissertations
The sphingolipid, ceramide-1-phosphate (C1P), directly binds and activates Group IVA cytosolic phospholipase A2 (cPLA2a) to generate eicosanoids. Due to the role of eicosanoids in wound healing, we choose to use our novel genetic mouse model expressing cPLA2a with an ablated C1P interaction site (KI) to examine the cPLA2a/C1P interaction in wound healing. Wound closure rate was not affected, but wound maturation was dramatically enhanced by loss of the C1P/cPLA2α interaction based on the following findings. Wounds in KI mice displayed: i) increased infiltration of dermal fibroblasts into the wound environment; ii …
Processing Of 3′-Blocked Dna Double-Strand Breaks By Tyrosyl-Dna Phosphodiesterase 1, Artemis And Polynucleotide Kinase/ Phosphatase, Ajinkya S. Kawale
Processing Of 3′-Blocked Dna Double-Strand Breaks By Tyrosyl-Dna Phosphodiesterase 1, Artemis And Polynucleotide Kinase/ Phosphatase, Ajinkya S. Kawale
Theses and Dissertations
DNA double-strand breaks (DSBs) containing unligatable termini are potent cytotoxic lesions leading to growth arrest or cell death. The Artemis nuclease and tyrosyl-DNA phosphodiesterase (TDP1) are each capable of resolving protruding 3′-phosphoglycolate (PG) termini of DNA double-strand breaks (DSBs). Consequently, a knockout of Artemis and a knockout/knockdown of TDP1 rendered cells sensitive to the radiomimetic agent neocarzinostatin (NCS), which induces 3′-PG-terminated DSBs. Unexpectedly, however, a knockdown or knockout of TDP1 in Artemis-null cells did not confer any greater sensitivity than either deficiency alone, indicating a strict epistasis between TDP1 and Artemis. Moreover, a deficiency in Artemis, but not TDP1, resulted …
Cellular Inhibitor Of Apoptosis Protein2 – A Critical Regulator Of Neuroinflammation, Debolina Dipankar Biswas
Cellular Inhibitor Of Apoptosis Protein2 – A Critical Regulator Of Neuroinflammation, Debolina Dipankar Biswas
Theses and Dissertations
Inhibitors of apoptosis (IAPs) modulate cell death and play critical role in signal transduction that promotes inflammation. Recently, Smac mimetics, which are IAP antagonists, have attracted attention as novel cancer therapeutics. Cellular Inhibitor of Apoptosis 2 (cIAP2), a member of IAP family, positively affects both NF-κB and MAPK activation in response to many inflammatory stimuli. We observed that the lack of cIAP2 ablates LPS-induced neuroinflammation. Also, cIAP2-/- macrophages demonstrated diminished antigen presentation potential that could contribute to ablated immunity. In addition to these functions, we have previously reported that cIAP2 also regulates the activation of Interferon Regulatory Factor 1 …
The Distinction Of The Interactions Between The Transmembrane Domains Of Basigin Gene Products And Monocarboxylate Transporters, Joseph D. Fong
The Distinction Of The Interactions Between The Transmembrane Domains Of Basigin Gene Products And Monocarboxylate Transporters, Joseph D. Fong
UNF Graduate Theses and Dissertations
Although it was once thought that neurons solely rely on glucose as a substrate for cellular energy production, it is now known that small monocarboxylate molecules, like pyruvate, lactate, and ketone bodies, are also utilized. Monocarboxylates are transported across plasma membranes via facilitated diffusion using a family of transport proteins known as monocarboxylate transporters (MCTs). Four MCTs (MCT1, MCT2, MCT3, and MCT4) are expressed within neural tissues. Expression of the MCTs has been tied to co-expression of a cell adhesion molecule belonging to the Basigin subset of the immunoglobulin superfamily (IgSF). Basigin gene products are known to interact with MCT1 …
Antibiotic Effects Of Dimethyl Sulfoxide And Synthetic Polyphenols On E. Coli Cells, Camila Cardenas
Antibiotic Effects Of Dimethyl Sulfoxide And Synthetic Polyphenols On E. Coli Cells, Camila Cardenas
Theses and Dissertations
There is increasing interest in polyphenols due to their potential medicinal and antibiotic applications. Naturally occurring polyphenols, such as curcumin, have garnered tremendous attention. However, synthetic polyphenols commonly used in biochemistry experiments have not been sufficiently explored. The purpose of this study is to determine the effect of dimethyl sulfoxide on the cellular uptake of synthetic polyphenols. Three synthetic polyphenols (Phenol Red, Congo Red, and Bromophenol Blue) were tested for their antibiotic properties and compared to the antibiotic activity of curcumin. Our results show that phenol red does not interact with the bacterial membrane even in the presence of 30% …
New Insights Into The "Manna From Heaven" Hypothesis, Clare Laubenthal
New Insights Into The "Manna From Heaven" Hypothesis, Clare Laubenthal
Honors Program Theses
Titan’s atmosphere is unique in that its atmospheric chemistry is thought to be similar to that of the Earth’s atmosphere during the Archean Era, when bacterial life dominated the Earth1. Tholins form as a result of the radiation of organic gases by ultraviolet light. These molecules are considered alike to the prebiotic hazes that formed in the atmosphere of Earth during the Archean Era and which allowed for the eventual development of microbial life2 . This study investigated whether Earth soil bacteria can grow, using laboratory created tholin analogs, or aerosols, as a nutrient source. This study …
Prebiotic Potential Of Aerosols, Emma Rae Shipley
Prebiotic Potential Of Aerosols, Emma Rae Shipley
Honors Program Theses
A triple quadrupole gas chromatograpy mass spectrometer (GC/MS/QQQ) was used to determine prebiotic molecules of interest in plasma and photochemical aerosols. Glycolic acid was further studied using isotopically doped gases. Plasma and photochemical aerosol analogs were shown to have nucleobases and a few amino acids. Each type of aerosol was also the source of glycolic acid, a precursor to glycine. Using 18O doped CO, it was determined that contamination was not a source of oxygen in the samples. Using different combinations of 13C doped gases in 1:1 combinations of CO and CH4, it was determined that …