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Biochemistry Commons

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1965

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Articles 1 - 11 of 11

Full-Text Articles in Biochemistry

The Role Of Thiamine Pyrophosphate In Regulating The Level Of Gamma-Aminobutyric Acid In The Central Nervous System, Bryant L. Adams Aug 1965

The Role Of Thiamine Pyrophosphate In Regulating The Level Of Gamma-Aminobutyric Acid In The Central Nervous System, Bryant L. Adams

Theses and Dissertations

The level of gamma-aminobutyric acid in the brains of white rats suffering from thiamine deficiency symptoms resulting from pyrithiamine or oxythiamine antagonism of thiamine or from thiamine deprivation was measured. The level of this amino acid was 17.5% lower in the brains of pyrithiamine-treated rats and 20.0% lower in the brains of thiamine-deprived rats than in the control rats. Daily injections of ten times the required amounts of vitamin B6 in rats treated with pyrithiamine or oxythiamine or by thiamine deprivation had no effect on the level of brain gamma-aminobutyric acid or on the psiological behavior of these rats. The …


Studies Of Rat Brain Thiamine Pyrophosphokinase, Lavell Rolfson Johnson Aug 1965

Studies Of Rat Brain Thiamine Pyrophosphokinase, Lavell Rolfson Johnson

Theses and Dissertations

The enzyme ATP:thiamine pyrophosphotransferase (thiamine pyrophosphokinaseJ was purified from acetone powders which were prepared from rat brains up to the chromatography on alumina C_γ step by a modified procedure that Mano (Y. Mano, J. Biochem., 42, 283 (1960)] developed for the purification of the rat liver thiamine pyrophosphokinase. The enzyme was purified 12 fold over that in the original extract of the acetone powder. A spectrophotometric assay for the brain thiamine pyrophosphokinase was developed utilizing brewer's yeast apotransketolase. The transketolase assay was able to detect less than 10^-11 moles of thiamine diphosphate, which was synthesized by the kinase, in the …


Water Chemistry Survey Of Boulder Basin, Lake Mead, P. R. Tramutt, Bureau Of Reclamation Jun 1965

Water Chemistry Survey Of Boulder Basin, Lake Mead, P. R. Tramutt, Bureau Of Reclamation

Publications (WR)

The survey results indicate that the impoundment of water behind Hoover Dam has not adversely affected the dissolved oxygen (DO) content and that water quality and DO content were uniform regardless of depth. The study made in April-May 1964 will provide water quality data of Lake Mead prior to releases from Lake Powell as a basis for evaluating Lake Powell's effect on water quality and limnology of Lake Mead. The performance of a DO analyzer was tested and found unsatisfactory at depths below 150 ft. Parameters tested by standard chemical analyses of water samples in the Denver Laboratory and by …


A Study Of 5-Fluorouracil: Its Effects And Mode Of Action In Drosophila Melanogaster, John B. Jenkins Jr. May 1965

A Study Of 5-Fluorouracil: Its Effects And Mode Of Action In Drosophila Melanogaster, John B. Jenkins Jr.

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Investigations involving the fluorinated pyrimidine, 5-fluorouracil, and its effects on viruses, bacteria, plants and mammals, have brought to light the following facts: FU inhibits the growth of bacteria, plants and mammalian cells and it decreases the ability of certain viruses to produce progeny. In other words, FU is an antimetabolite that inhibits growth and/or reproduction. Two mechanisms have emerged from these studies to define the mode of action of FU. The first mechanism involves the incorporation of the compound into the RNA of the organism thus altering the base sequence and creating a non functional or damaging nucleic acid. The …


Interrelationships Of Certain Metabolic Responses To Threonine Deficiency And To Various Dietary Carbohydrates In The White Rat, Elizabeth Bright Jan 1965

Interrelationships Of Certain Metabolic Responses To Threonine Deficiency And To Various Dietary Carbohydrates In The White Rat, Elizabeth Bright

Journal of the Arkansas Academy of Science

No abstract provided.


Effects Of Saturated And Unsaturated Long Chain Fatty Acids On Various Components Of Lipid And Carbohydrate Metabolism, Joyce T. Burgess Jan 1965

Effects Of Saturated And Unsaturated Long Chain Fatty Acids On Various Components Of Lipid And Carbohydrate Metabolism, Joyce T. Burgess

Journal of the Arkansas Academy of Science

No abstract provided.


Effect Of Dietary Galactose Vs. Glucose With Corn Oil And With Hydrogenated Coconut Oil On Various Metabolic Functions In Rats, Mabel R. Coleman, Catherine Carroll Jan 1965

Effect Of Dietary Galactose Vs. Glucose With Corn Oil And With Hydrogenated Coconut Oil On Various Metabolic Functions In Rats, Mabel R. Coleman, Catherine Carroll

Journal of the Arkansas Academy of Science

No abstract provided.


A Quantitative Assay For Vanillylmandelic Acid (Vma) By Gas-Liquid Chromatography / Sherwin Wilk From The Department Of Medicine, Th Emount Sinai Hospital, New York, New York And The Department Of Chemistry, Fordham University, New York, N.Y. Stanley E. Gitlow And Milton Mendlowitz From The Department Of Medicine, The Mount Sinai Hospital, New York, New York Morton J. Franklin And Herman E. Carr From The Department Of Psychiatry, Boston University, School Of Medicine, Boston, Massachusetts And Donald D. Clarke From The Department Of Chemistry, Fordham University, New York, New York, Sherwin Wilk, Stanley E. Gitlow, Milton Mendlowitz, Donald Dudley Clarke Phd Jan 1965

A Quantitative Assay For Vanillylmandelic Acid (Vma) By Gas-Liquid Chromatography / Sherwin Wilk From The Department Of Medicine, Th Emount Sinai Hospital, New York, New York And The Department Of Chemistry, Fordham University, New York, N.Y. Stanley E. Gitlow And Milton Mendlowitz From The Department Of Medicine, The Mount Sinai Hospital, New York, New York Morton J. Franklin And Herman E. Carr From The Department Of Psychiatry, Boston University, School Of Medicine, Boston, Massachusetts And Donald D. Clarke From The Department Of Chemistry, Fordham University, New York, New York, Sherwin Wilk, Stanley E. Gitlow, Milton Mendlowitz, Donald Dudley Clarke Phd

Chemistry Faculty Publications

Until the discovery of the major product of catecholamine n1etabolism, vanillylmandelic acid (VMA), by Annstrong in 1957 (1), biochemical studies of epinephrine and norepinephrine metabolism were dependent upon determination of the small quantities of these materials excreted unchanged in the urine. The methods designed to measure VMA have proved adequate to detect the gross abnormalities in metabolism usually associated with catecholamine-producing tumors (2-7), but more exact procedures are required for quantitative metabolic studies. Present spectrophotometric and electrophoretic methods are nonspecific in that they yield values for VMA that are higher than those obtained by chromatographic and isotope dilution techniques (5). …


The Distribution Of Hydrolytic Enzymes And Ribonucleic Acid In Subcellular Fractions From Eggs And Adult Tissue Of Arbacia Punctulata, Charlotte Jackson Jan 1965

The Distribution Of Hydrolytic Enzymes And Ribonucleic Acid In Subcellular Fractions From Eggs And Adult Tissue Of Arbacia Punctulata, Charlotte Jackson

Dissertations, Theses, and Masters Projects

No abstract provided.


Effect Of Environmental Salinity On The Amino Acids Of Balanus Improvisus Darwin, Joan L. Faunce Jan 1965

Effect Of Environmental Salinity On The Amino Acids Of Balanus Improvisus Darwin, Joan L. Faunce

Dissertations, Theses, and Masters Projects

No abstract provided.


The Effects Of Environmental Salinity On Free Amino Acids Of Crassostrea Virginica, Maurice Patrick Lynch Jan 1965

The Effects Of Environmental Salinity On Free Amino Acids Of Crassostrea Virginica, Maurice Patrick Lynch

Dissertations, Theses, and Masters Projects

No abstract provided.