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Full-Text Articles in Biochemistry
Development Of Catalytic Rnas As Potential Anti-Hiv-1 Agents, Pairoj Sae Chang
Development Of Catalytic Rnas As Potential Anti-Hiv-1 Agents, Pairoj Sae Chang
Loma Linda University Electronic Theses, Dissertations & Projects
RNA molecules with enzymatic activity (ribozymes) targeted to the genome of HIV-1 were developed and demonstrated to mediate site-specific cleavage of the HIV-1 RNA in vitro and to inhibit viral proliferation intracellularly. Two classes of ribozymes that incorporate a "hammerhead" catalytic center were utilized, eotide elements in the viral sequence to provide half of the The first class used nuclcatalytic center. A complementary strand harboring the remaining active site nucleotides was supplied in trans and directed the site-specific cleavage of the HIV-1 RNA target. A second type of ribozyme incorporated the entire catalytic center, minus the site of cleavage, within …