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Full-Text Articles in Biochemistry
Towards Elucidation Of A Viral Dna Packaging Motor, Chad T. Schwartz
Towards Elucidation Of A Viral Dna Packaging Motor, Chad T. Schwartz
Theses and Dissertations--Pharmacy
Previously, gp16, the ATPase protein of phi29 DNA packaging motor, was an enigma due to its tendency to form multiple oligomeric states. Recently we employed new methodologies to decipher both its stoichiometry and also the mechanism in which the protein functions to hydrolyze ATP and provide the driving force for DNA packaging. The oligomeric states were determined by biochemical and biophysical approaches. Contrary to many reported intriguing models of viral DNA packaging, it was found that phi29 DNA packaging motor permits the translocation of DNA unidirectionally and driven cooperatively by three rings of defined shape. The mechanism for the generation …