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Articles 31 - 31 of 31
Full-Text Articles in Biochemistry
Exploitation Of The Bacteriophage-Derived Peptidase Chapk, Pierre-Mehdi Hadbi
Exploitation Of The Bacteriophage-Derived Peptidase Chapk, Pierre-Mehdi Hadbi
Theses
This project investigated an anti-staphylococcal bacteriophage-derived peptidoglycan hydrolase enzyme, namely a cysteine-histidine amydohydrolase/peptidase (CHAPk). The study focused firstly on optimizing the production of recombinant CHAPk, which was previously cloned in an E. coli expression system, and sets out the resulting optimal Standard Operating Procedures (SOPs) for production, purification and determination of concentration and activity of the resulting protein stock. A typical yield resulting from the investigation was 300pg of CHAPk from one litre of E.coli culture. The activity of the purified enzyme was typically in the region of 190U nmol ’. Secondly, the project investigated …