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Articles 31 - 60 of 101
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
The Relationship Between Antibody Redox Structure And Affinity In Rainbow Trout, Jianmin Ye
The Relationship Between Antibody Redox Structure And Affinity In Rainbow Trout, Jianmin Ye
Dissertations, Theses, and Masters Projects
Teleost immunoglobulin M (IgM), an 800 kDa tetramer, possesses considerable structural diversity due to the non-uniform disulfide polymerization of its halfmeric or monomeric subunits. However, to date, no plausible functional role for this diversity has been demonstrated or proposed. This research was, therefore, designed to investigate the possible functional role(s) for this diversity using the trout model. The possible relationship between this structural diversity and affinity was specifically addressed. The relationship between high levels of disulfide polymerization and high affinity was demonstrated by selective immunoadsorption and analysis of antibodies isolated during the process of affinity maturation. A pivotal determinative role …
Analysis Of Sequence Variation At Two Helicobacter Pylori Genetic Loci Potentially Involved In Virulence, George Warren Liechti
Analysis Of Sequence Variation At Two Helicobacter Pylori Genetic Loci Potentially Involved In Virulence, George Warren Liechti
Dissertations, Theses, and Masters Projects
No abstract provided.
Stable Isotope Dynamics In Summer Flounder Tissues, With Application To Dietary Assessments In Chesapeake Bay, Andre Buchheister
Stable Isotope Dynamics In Summer Flounder Tissues, With Application To Dietary Assessments In Chesapeake Bay, Andre Buchheister
Dissertations, Theses, and Masters Projects
Stable isotope techniques were applied to summer flounder, Paralichthys dentatus, in Chesapeake Bay to elucidate the relative importance of different prey groups on the growth and productivity of this species. Prior to field application, a laboratory diet-shift study was conducted to evaluate methodological assumptions and obtain necessary isotopic parameters. Specifically, the goals of the laboratory study were to 1) determine isotopic turnover rates and fractionations of δ13C and δ15N in liver, whole blood, and white muscle and 2) estimate the relative importance of growth and metabolic processes on isotopic turnover. Groups of captive juvenile summer flounder (130-255mm total length) were …
A Molecular Phylogeny Of The Genus Bonamia Based On Sequence Data Of The Ribosomal Rna (Rrna) Gene Complex, Delonna M. White
A Molecular Phylogeny Of The Genus Bonamia Based On Sequence Data Of The Ribosomal Rna (Rrna) Gene Complex, Delonna M. White
Dissertations, Theses, and Masters Projects
No abstract provided.
Markov Chain Models Of Calcium Puffs And Sparks, Jeffrey R. Groff
Markov Chain Models Of Calcium Puffs And Sparks, Jeffrey R. Groff
Dissertations, Theses, and Masters Projects
Localized cytosolic Ca2+ elevations known as puffs and sparks are important regulators of cellular function that arise due to the cooperative activity of Ca2+-regulated inositol 1,4,5-trisphosphate receptors (IP3Rs) or ryanodine receptors (RyRs) co-localized at Ca2+ release sites on the surface of the endoplasmic reticulum or sarcoplasmic reticulum. Theoretical studies have demonstrated that the cooperative gating of a cluster of Ca2+-regulated Ca 2+ channels modeled as a continuous-time discrete-state Markov chain may result in dynamics reminiscent of Ca2+ puffs and sparks. In such simulations, individual Ca2+-release channels are coupled via a mathematical representation of the local [Ca2+] and exhibit "stochastic Ca2+ …
Markov Chain Models Of Instantaneously Coupled Intracellular Calcium Channels, Hilary Deremigio
Markov Chain Models Of Instantaneously Coupled Intracellular Calcium Channels, Hilary Deremigio
Dissertations, Theses, and Masters Projects
Localized calcium elevations known as calcium puffs or sparks are cellular signals arising from cooperative activity of clusters of inositol 1,4,5-trisphosphate receptors (IP3Rs) or ryanodine receptors (RyRs) located at calcium release sites on the endoplasmic or sarcoplasmic reticulum membrane. When Markov chain models of these intracellular calcium-regulated calcium channels are coupled via a mathematical representation of the calcium microdomain, simulated calcium release sites may exhibit the phenomenon of "stochastic calcium excitability" where the IP3Rs or RyRs open and close in a concerted fashion. Although the biophysical theory relating the kinetics of single channels to the collective phenomena of puffs and …
A Molecular Study Of The Mitochondrial Genome And Invasions Of The Veined Rapa Whelk, Rapana Venosa, Emily A. Chandler
A Molecular Study Of The Mitochondrial Genome And Invasions Of The Veined Rapa Whelk, Rapana Venosa, Emily A. Chandler
Dissertations, Theses, and Masters Projects
No abstract provided.
Effects Of Molecular Motion On Deuteron Magic Angle Spinning Nmr Spectra, Yuanyuan Huang
Effects Of Molecular Motion On Deuteron Magic Angle Spinning Nmr Spectra, Yuanyuan Huang
Dissertations, Theses, and Masters Projects
Solid state deuteron NMR experiments, especially magic angle spinning (MAS) and off-magic angle spinning (OMAS), are developed to explore dynamical systems. A theoretical discussion of interactions relevant for spin-1 nuclei is presented. Practical aspects of MAS/OMAS experiments are described an detail. The dominant quadrupolar coupling interaction in deuteron NMR has been simulated and the effects of multiple-frame molecular motions on MAS/OMAS spectra are taken into account in this calculation. Effects of chemical shift anisotropy are also simulated, and shown to be small under conditions of rapid sample spinning.;Two numerical methods, direct integration and an efficient simulation routine based on Floquet …
A Molecular Technique For Specific Identification Of Western Atlantic Ocean Scombrids And An Analysis Of A Larval Scombrid Assemblage Off The Kona Coast Of Hawaii, Melissa A. Paine
A Molecular Technique For Specific Identification Of Western Atlantic Ocean Scombrids And An Analysis Of A Larval Scombrid Assemblage Off The Kona Coast Of Hawaii, Melissa A. Paine
Dissertations, Theses, and Masters Projects
No abstract provided.
Effects Of Overexpressing Alternative Isoform Pax-5e In Vivo, Suzanne Elizabeth Cole
Effects Of Overexpressing Alternative Isoform Pax-5e In Vivo, Suzanne Elizabeth Cole
Dissertations, Theses, and Masters Projects
No abstract provided.
Genetic Variation Within And Among The Suminoe Oyster (Crassostrea Ariakensis) Populations And Stocks As Assessed By Molecular Markers, Qian Zhang
Dissertations, Theses, and Masters Projects
No abstract provided.
Evolution And Analysis Of A Catalytically Effective Model Enzyme: The Importance Of Active Site Orientation And Tuned Conformational Fluctuations, G. S. Blair Williams
Evolution And Analysis Of A Catalytically Effective Model Enzyme: The Importance Of Active Site Orientation And Tuned Conformational Fluctuations, G. S. Blair Williams
Dissertations, Theses, and Masters Projects
No abstract provided.
Characterization Of The Xgat-1 Gene In Xenopus Laevis, Lisa Layne August
Characterization Of The Xgat-1 Gene In Xenopus Laevis, Lisa Layne August
Dissertations, Theses, and Masters Projects
No abstract provided.
A Mycobacterium-Inducible Nramp In Striped Bass Morone Saxatilis, Erin Jeffrey Burge
A Mycobacterium-Inducible Nramp In Striped Bass Morone Saxatilis, Erin Jeffrey Burge
Dissertations, Theses, and Masters Projects
In mammals, the natural resistance-associated macrophage protein 1 gene, Nramp1, plays a major role in resistance to mycobacterial infections. Chesapeake Bay (USA) striped bass, Morone saxatilis, are currently experiencing an epizootic of mycobacteriosis that threatens the health of this ecologically and economically important species. This dissertation characterizes an Nramp gene in this species (MsNramp ) and provide evidence for induction following Mycobacterium exposure. The striped bass MsNramp gene and 554 amino acid sequence contain all the signal features of the Nramp family, including a topology of 12 transmembrane domains (TM), the transport protein specific 'binding-protein-dependent transport system inner membrane component …
Synalpheus Shrimp From Carrie Bow Cay, Belize. Systematics, Phylogenetics And Biological Observations (Crustacea: Decapoda: ; Alpheidae), Ruben Rios Gonzalez
Synalpheus Shrimp From Carrie Bow Cay, Belize. Systematics, Phylogenetics And Biological Observations (Crustacea: Decapoda: ; Alpheidae), Ruben Rios Gonzalez
Dissertations, Theses, and Masters Projects
Snapping shrimp in the family Alpheidae are remarkably abundant in coral reef ecosystems worldwide. The second most speciose genus, Synalpheus , includes species usually small and reclusive, and their general morphology is notably uniform, yet the number of species is staggeringly high, as is the intraspecific variability. These features have contributed to a poorly resolved taxonomic status, as evidenced both by recurring lumping and splitting of species and by lack of proper definition of several common species. I examined western Atlantic species of Synalpheus in the large and regionally dominant "Gambarelloides group of species", an informal but widely used guild …
Cloning And Functional Characterization Of Hypoxia-Inducible Factor 1alpha Upstream Regions In Xenopus Laevis, Conor W. Sipe
Cloning And Functional Characterization Of Hypoxia-Inducible Factor 1alpha Upstream Regions In Xenopus Laevis, Conor W. Sipe
Dissertations, Theses, and Masters Projects
No abstract provided.
Genetic Stock Structure Of The Sailfish, Istiophorus Platypterus, Based On Nuclear And Mitochondrial Dna, Jan Renee Mcdowell
Genetic Stock Structure Of The Sailfish, Istiophorus Platypterus, Based On Nuclear And Mitochondrial Dna, Jan Renee Mcdowell
Dissertations, Theses, and Masters Projects
The results of life history studies involving the sailfish, Istiophorus platypterus, from the Atlantic, Pacific, and Indian oceans were reviewed. Results of studies agree on several aspects of sailfish biology; sailfish exhibit opportunistic feeding behavior, follow the 28??C isotherm, spawn in summer mature at approximately age 3, and are the least migratory of the billfishes. However, results are in conflict concerning sex ratio and whether sailfish are single or multiple spawners. Although many researchers have reported that sailfish are sexually dimorphic, their fragile otoliths make them difficult to age, aging studies have not been validated, and sexual dimorphism has not …
Chromosome Set Instability In 1--2 Year Old Triploid Crassostrea Ariakensis In Multiple Environments, Mingfang Zhou
Chromosome Set Instability In 1--2 Year Old Triploid Crassostrea Ariakensis In Multiple Environments, Mingfang Zhou
Dissertations, Theses, and Masters Projects
No abstract provided.
A Dynamical Perspective On Enzymatic Catalysis, Shiying Shang
A Dynamical Perspective On Enzymatic Catalysis, Shiying Shang
Dissertations, Theses, and Masters Projects
No abstract provided.
Molecular And Morphological Evolution Of The Amphipod Radiation Of Lake Baikal, Kenneth S. Macdonald Iii
Molecular And Morphological Evolution Of The Amphipod Radiation Of Lake Baikal, Kenneth S. Macdonald Iii
Dissertations, Theses, and Masters Projects
The amphipods of Lake Baikal, Russia, are a taxonomically and morphologically diverse group, and have been cited as a classic example of adaptive radiation. An unfortunate consequence of this diversity has been an instability of Baikal amphipod classification, with frequent revisions spurred by differing opinions about the phylogenetic utility of different characters. Classifications have never been based on a rigorous phylogenetic analysis of morphological data and only recently has the phylogeny of Lake Baikal's amphipods been examined using molecular tools. Unfortunately, most of these studies have led to a limited expansion of our understanding of Baikal amphipod systematics. Phylogenetic relationships …
Molecular Characterization Of Cultured Perkinsus Marinus Isolates, Gwynne D. Brown
Molecular Characterization Of Cultured Perkinsus Marinus Isolates, Gwynne D. Brown
Dissertations, Theses, and Masters Projects
Perkinsus marinus is the causative agent of the oyster disease Dermo in the eastern oyster, Crassostrea virginica. In vitro propagation of the parasite has led to the establishment of multiple isolates by several investigators. Little work, however, has been done to characterize different isolates. In this study multiple isolates were examined for genetic and biochemical diversity. at two loci, the ITS region and the ATAN region, there was as much intra-isolate genetic variation among DNA sequences of some isolates as there was inter-isolate variation. Variation was also observed at a third loci, a subtilisin-like serine protease gene. This is the …
Adrenal Expression Of Cyp17 And Hsd3b1 Mrna In Peromyscus Maniculatus Bairdii, Brian Thomas Lovitt
Adrenal Expression Of Cyp17 And Hsd3b1 Mrna In Peromyscus Maniculatus Bairdii, Brian Thomas Lovitt
Dissertations, Theses, and Masters Projects
No abstract provided.
Function And Regulation Of Alternative Isoforms Of The Transcription Factor Pax-5 During B Lymphocyte Differentiation, Marina A. Lowen
Function And Regulation Of Alternative Isoforms Of The Transcription Factor Pax-5 During B Lymphocyte Differentiation, Marina A. Lowen
Dissertations, Theses, and Masters Projects
No abstract provided.
The Specific Immune Response In Rainbow Trout: Somatic Hypermutation And Vh Gene Utilization, Teresa D. Lewis
The Specific Immune Response In Rainbow Trout: Somatic Hypermutation And Vh Gene Utilization, Teresa D. Lewis
Dissertations, Theses, and Masters Projects
The study of antibody responses in prominent aquaculture species such as the rainbow trout, Oncorhynchus mykiss, can facilitate vaccine development and contribute to producing useful paradigms of adaptive immunity in lower vertebrates. Thus, it is essential to identify genes responsible for antibody responses. In the mouse model, hybridoma technology allows for the association of monoclonal antibodies possessing various affinities for antigen with specific VH sequences, gene family utilization, and other molecular events (i.e. somatic hypermutation) that occur during the specific immune response. The absence of a comparable hybridoma technology in piscine systems has limited similar studies of fish immunogenetics to …
Environmental Stress In Hard Coral: Evaluating Lipid As An Indicator Of Sublethal Stress On Short Time Scales, David Harold Niebuhr
Environmental Stress In Hard Coral: Evaluating Lipid As An Indicator Of Sublethal Stress On Short Time Scales, David Harold Niebuhr
Dissertations, Theses, and Masters Projects
Lipid quality was evaluated in Montastrea spp. under sediment- and heat-stressed conditions to evaluate lipid ratio as an indicator of sub-lethal stress on short time scales. The ratio of storage lipid (wax ester + triacylglyceride) to structural lipid (sterol esters + phospholipid) decreased significantly (0.25 to 0.14, p < 0.01) after experimental sedimentation. FAME analysis of colonies exposed to experimental sedimentation showed a reduction of the algal, 18:3(n-6) and 18:4(n-3), polyunsaturated fatty acid (PUFA) in the polar lipid fraction. This loss of PUFA suggests a loss of algal membrane in sediment-stressed colonies. Lipid quality was similarly measured in M. faveolata over a 10-day period. Mean (n = 20) ratio of storage to structural lipid in M. faveolata dropped from 2.43 to a level of 0.98 immediately following a natural sedimentation event before recovering to levels of 1.4 and 2.9 on post-storm days 2 and 4, respectively. Colonies of M. annularis subject to heat-stress (35??C) exhibited no significant change in storage lipid ratio, while levels of Free Fatty Acids increased significantly from 0.012 (n = 22) to 0.156 mg lipid/g dry tissue (n = 22)(p < 0.05). FAME analysis of tissue lipids extracted from the heat-stressed colonies showed changes in the polar fraction, with significant decreases in the 18:3(n-6), 18:3(n-3), 18:4(n-3), 20:4(n-6) and 20:5(n-3) (p < 0.05) PUFA and subsequent significant increases in the saturated fatty acids, 16:0 and 18:0 (p < 0.05). These changes in lipid quantity and quality indicate possible oxidation and preferential digestion of zooxanthellar membranes. Stress experiments were repeated in M. annularis using VacutainerRTM blood collection tubes to collect micro-tissue samples without destroying skeleton of the sample colonies. A significant decrease in storage: structural lipid ratio after sedimentation was also detected using the micro-tissue technique. This study indicates that the relative abundance of lipid subclass components can indicate sub-lethal environmental stress, on short time scales, in M. annularis and M. faveolata. Furthermore, micro-tissue collection techniques permit repeated monitoring coral colonies to assess the manifestation of stress from first detection of impact at the cellular level to changes in community to changes in community structure detectable over longer time scales.
32p-Postlabeling Analysis Of Aromatic Dna Adducts In Hemopoietic Tissues And Blood Of The Mummichog Fundulus Heteroclitus, Wendy L. Rose
32p-Postlabeling Analysis Of Aromatic Dna Adducts In Hemopoietic Tissues And Blood Of The Mummichog Fundulus Heteroclitus, Wendy L. Rose
Dissertations, Theses, and Masters Projects
No abstract provided.
Functional And Molecular Analysis Of Antibody Affinity Maturation In Rainbow Trout (Oncorhynchus Mykiss), Haili Zhang
Functional And Molecular Analysis Of Antibody Affinity Maturation In Rainbow Trout (Oncorhynchus Mykiss), Haili Zhang
Dissertations, Theses, and Masters Projects
No abstract provided.
Indo-Pacific Population Structure Of The Black Marlin, Makaira Indica, Inferred From Molecular Markers, Brett Falterman
Indo-Pacific Population Structure Of The Black Marlin, Makaira Indica, Inferred From Molecular Markers, Brett Falterman
Dissertations, Theses, and Masters Projects
No abstract provided.
Molecular Phylogeny Of Lake Baikal Amphipods, Kenneth S. Macdonald
Molecular Phylogeny Of Lake Baikal Amphipods, Kenneth S. Macdonald
Dissertations, Theses, and Masters Projects
No abstract provided.
The Phylogeny Of Coleoid Cephalopods Inferred From Molecular Evolutionary Analyses Of The Cytochrome C Oxidase I, Muscle Actin, And Cytoplasmic Actin Genes, David Bruno Carlini
The Phylogeny Of Coleoid Cephalopods Inferred From Molecular Evolutionary Analyses Of The Cytochrome C Oxidase I, Muscle Actin, And Cytoplasmic Actin Genes, David Bruno Carlini
Dissertations, Theses, and Masters Projects
Although the fossil record of early cephalopods is rich and demonstrates the dominance of the group in Paleozoic times, the mainly soft-bodied coleoids (Cephalopoda: Coleoidea) are poorly represented. Therefore, little is known of the evolutionary history of coleoids through paleontology and current classifications of the subclass are based primarily on the morphology of extant representatives. A molecular phylogenetic analysis of the Coleoidea was therefore warranted. Phylogenetic relationships within the Coleoidea were constructed using molecular sequence data from one mitochondrial and two nuclear genes: cytochrome c oxidase I (COI) and two unlinked actin genes (Actin I and Actin II, respectively). A …