Meat Science Commons^™

Microbial Community Structure From Southern High Plains Beef Cattle Feedyard Manure And Relationship With Nitrous Oxide Emissions, Heidi Waldrip, David Parker, Sierra Miller, Lisa M. Durso, Byeng R. Min, Daniel N. Miller, Kenneth Casey, Bryan Woodbury, Mindy J. Spiehs

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Modern molecular techniques enable characterization of the microbial biome in livestock manure, from which there is particular concern over emission of greenhouse gases. This study evaluated how sampling depth, time, temperature, and artificial rainfall affected microbial community structure in feedyard manure, and relationships between the manure biome and known parameters related to nitrous oxide (N₂O) emissions. In three large incubation chambers, maintained at different temperatures that received two applications of artificial rainfall, we evaluated manure microbiome composition and abundance of N₂O-producing enzymes (nirK and nirS) using quantitative polymerase chain reaction (qPCR). These data were …

Inosine 5’- Monophosphate Derived Umami Flavor Intensity Of Beef Determination By Electrochemistry And Chromatography, Kezia Virellia To

Theses and Dissertations

The umami sensation contributes to beef flavor and acceptability. Inosine 5’- monophosphate (IMP) was the most abundant nucleotide in meat known to impart umami taste which thus far had been overlooked in meat flavor studies. The objectives of this study were to determine the umami taste threshold of inosine 5'-monophosphate (IMP), the effects of spiking IMP on the sensory descriptive attributes of various USDA graded beef strip steaks, and to differentiate beef by IMP content using electrochemistry. USDA Prime, Choice, and Select steaks were spiked with 0.3 and 0.6 mM IMP and analyzed chemically and organoleptically. Liquid chromatography-mass spectrometry and …

Evaluating The Shelf Life And Sensory Properties Of Beef Steaks From Cattle Raised On Different Grass Feeding Systems In The Western United States, Toni L. Duarte, Bakytzhan Bolkenov, Sarah C. Klopatek, James W. Oltjen, D. Andy King, Steven D. Shackelford, Tommy L. Wheeler, Xiang Yang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Consumer interest in grass-fed beef has been steadily rising due to consumer perception of its potential benefits. This interest has led to a growing demand for niche market beef, particularly in the western United States. Therefore, the objective of this study was to assess the impact of feeding systems on the change in microbial counts, color, and lipid oxidation of steaks during retail display, and on their sensory attributes. The systems included: conventional grain-fed (CON), 20 months-grass-fed (20GF), 25-months-grass-fed (25GF) and 20-months-grass-fed + 45-day-grain-fed (45GR). The results indicate that steaks in the 20GF group displayed a darker lean and fat …

Genes Involved In Feed Efficiency Identified In A Meta-Analysis Of Rumen Tissue From Two Populations Of Beef Steers, Amanda K. Lindholm-Perry, Allison M. Meyer, Rebecca J. Kern-Lunbery, Hannah C. Cunningham-Hollinger, Taran H. Funk, Brittney N. Keel

Roman L. Hruska U.S. Meat Animal Research Center: Reports

In cattle, the rumen is an important site for the absorption of feed by-products released by bacterial fermentation, and variation in ruminal function plays a role in cattle feed efficiency. Studies evaluating gene expression in the rumen tissue have been performed prior to this. However, validating the expression of genes identified in additional cattle populations has been challenging. The purpose of this study was to perform a meta-analysis of the ruminal transcriptome of two unrelated populations of animals to identify genes that are involved in feed efficiency across populations. RNAseq data from animals with high and low residual feed intake …

Prenatal Transportation Stress Did Not Impact Ovarian Follicle Count For Three Generations Of Female Brahman Offspring, Lacey K. Quail, Ronald D. Randel, Thomas H. Welsh Jr., Robert A. Cushman, Hannah K. Yake, Rui A. D'Orey Branco, Donald A. Neuendorff, Charles R. Long, George A. Perry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

As prenatal transportation stress altered behavior and adrenal glucocorticoid secretion of calves, we hypothesized that prenatal transportation stress would decrease ovarian reserve size and negatively impact female offspring fertility. The impact of prenatal transportation stress on ovarian follicle numbers in female offspring for three generations was studied. Brahman cows were transported for 2 h on day 60 ± 5, 80 ± 5, 100 ± 5, 120 ± 5, and 140 ± 5 of gestation. Ovaries were collected from offspring of transported or non-transported dams at multiple ages. Primordial, primary, secondary, and antral follicles were histologically analyzed. Antral follicle numbers were …

Efficacy Of Antimicrobial Interventions Used In Meat Processing Plants Against Antimicrobial Tolerant Non–Antibiotic-Resistant And Antibiotic-Resistant Salmonella On Fresh Beef, Norasak Kalchayanand, Sapna Chitlapilly Dass, Yangjunna Zhang, Eric L. Oliver, Bing Wang, Tommy L. Wheeler

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Salmonella is a common cause of foodborne illness in the United States, and several strains of Salmonella have been identified as resistant to antibiotics. It is not known whether strains that are antibiotic resistant (ABR) and that have some tolerance to antimicrobial compounds are also able to resist the inactivation effects of antimicrobial interventions used in fresh meat processing. Sixty-eight Salmonella isolates (non-ABR and ABR strains) were treated with half concentrations of lactic acid (LA), peracetic acid (PAA), and cetylpyridinium chloride (CPC), which are used in beef processing plants to screen for tolerant strains. Six strains each from non-ABR and …

Effects Of Administration Of A Growth Promoting Implant During The Suckling Phase Or At Weaning On Growth, Reproduction, And Ovarian Development In Replacement Heifers Grazing Native Range, Shelby L. Rosasco, Emily A. Melchior-Tiffany, Cierrah J. Kassetas, Shad H. Cox, Richard L. Dunlap, Jennifer A. Hernandez Gifford, Eric J. Scholljegerdes, Robert A. Cushman, Adam F. Summers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Management strategies utilized during pre-breeding development of replacement heifers can impact fertility and the ovarian reserve. Angus- Hereford crossbred heifers (n = 233) were utilized over a 3-yr period to determine the effects of administration of a growth promoting implant at either branding or weaning on growth, reproduction, and ovarian development. Heifer calves were randomly assigned to one of three treatments: 1) nonimplanted controls (CON; n = 79), 2) implanted at approximately 2 mo of age (average calf age = 58 d) with Synovex-C (BIMP, n = 82), or 3) implanted at approximately 7 mo of age (average calf …

Interaction Of Replacing Corn Silage With Soyhulls As A Roughage Source With Or Without 3% Added Wheat Straw In The Diet: Impacts On Intake, Digestibility, And Ruminal Fermentation In Steers Fed High-Concentrate Diets, Bryan W. Neville, Wayde J. Pickinpaugh, Lea J. Mittleider, Rebecca L. Moore, Kendall C. Swanson, Joel S. Caton

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Six ruminally cannulated steers [475.0 ± 49.6 kg initial body weight (BW)] were used in a 6 × 3 incomplete Latin square design (six treatments and three periods), to evaluate the impacts replacing of corn silage with pelleted soyhulls as roughage in high-concentrate finishing diets containing 30% modified distillers grains with solubles. Treatments were based on increasing dietary inclusion of soyhulls and consisted of: (1) Control (0), roughage supplied by dietary inclusion of 20% corn silage [dry matter (DM) basis]; (2) 50% replacement of corn silage with soyhulls (50); (3) 100% replacement of corn silage with soyhulls (100), and the …

Development And Validation Of High-Resolution Melting Assays For The Detection Of Potentially Virulent Strains Of Escherichia Coli O103 And O121, Frank J. Velez, Joseph M. Bosilevac, Sabine Delannoy, Patrick Fach, Ravinder Nagpal, Prashant Singh

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Virulent strains of Shiga toxin-producing Escherichia coli (STEC) serogroups O103 and O121 are considered adulterants in beef. Two high-resolution melting (HRM) real-time PCR assays were standardized for the specific detection and discrimination of potentially virulent and avirulent strains of E. coli O103 and O121. The O103 HRM assay offered the possibility to distinguish clearly STEC O103:H2 from STEC O103:H25. The two standardized assays were extensively validated using 215 pure culture strains, laboratory inoculated food samples, and naturally contaminated beef (n = 84) and pork (n = 84) enrichments collected from the red meat surveillance program. Both HRM assays showed 100% …

Impacts Of Added Roughage On Growth Performance, Digestibility, Ruminal Fermentation, And Ruminal Ph Of Feedlot Steers Fed Wheat-Based Feedlot Diets Containing 30% Modified Distillers Grains With Solubles, Wayde J. Pickinpaugh, Bryan W. Neville, Rebecca L. Moore, Joel S. Caton

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Two experiments were conducted to evaluate the inclusion rate roughage in wheat-based diets containing modified distillers grains with solubles (MDGS) on feedlot performance (Feedlot Experiment), as well as digestibility, ruminal pH, and ruminal fermentation characteristics (Digestibility Experiment). The feedlot experiment utilized 72 Angus steers (392 ± 46.3 kg initial body weight) which were randomly assigned to 1 of 12 pens, 3 pens per treatment, to evaluate feedlot performance and carcass characteristics. Dietary treatments were 1) control; 10% roughage, 2) 12% roughage, 3) 14% roughage, and 4) 16% roughage. The digestibility experiment used four ruminally and duodenally cannulated steers (393 ± …

Greater Numbers Of Antral Follicles In The Ovary Are Associated With Increased Concentrations Of Glucose In Uterine Luminal Fluid Of Beef Heifers, Alexandria P. Snider, Matthew S. Crouse, Shelby L. Rosasco, Kaitlin M. Epperson, Emmalee J. Northrop-Albrecht, Jerica J.J. Rich, Chadwick C. Chase Jr., Jeremy R. Miles, George A. Perry, Adam F. Summers, Robert A. Cushman

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Increased antral follicles are associated with greater fertility and a uterine environment that is more supportive of early embryonic development in beef heifers. Glucose is a primary energy source for embryos, and glucose concentrations are elevated in uterine luminal fluid (ULF) of pregnant heifers. We hypothesized that ULF glucose concentrations and endometrial transcript abundance for glucose transporters on d16 after insemination would be greater in heifers with increased numbers of antral follicles. Heifers classified with either increased or diminished antral follicle counts were artificially inseminated following the CO-Synch protocol (d0). On d16 after insemination, reproductive tracts of heifers were collected …

Proteomic Analyses Identify Differences Between Bovine Epididymal And Ejaculated Spermatozoa That Contribute To Longevity, Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie A. Walker, Robert A. Cushman, George A. Perry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Sperm are stored for extended periods of time in the epididymis, but upon ejaculation motility is increased and lifespan is decreased. The objective of this study was to identify differences in proteins between epididymis and ejaculated samples that are associated with longevity. Ejaculated semen was collected from mature Angus bulls (n = 9); bulls were slaughtered and epididymal semen was collected. Epididymal and ejaculated semen were centrifuged to separate sperm and fluid. Fluids were removed and sperm pellets were resuspended in a high ionic solution and vortexed to remove loosely attached proteins. Sperm samples were centrifuged, and the supernatant was …

Analysis Of Livestock Movements And The Benefits And Costs Of Livestock Standstills In Australia As Part Of The Response To Foot-And-Mouth Disease, Ann Seitzinger, Graeme Garner, Richard Bradhurst, Walter Okelo, Michelle Rodan

Animal production and livestock published reports

This project involved analysis of livestock movements and used epidemiological modelling and cost analysis to estimate the benefits and costs of implementing livestock standstills in response to a foot-and-mouth disease (FMD) outbreak in Australia. This was done in four steps: 1) assessing the extent of livestock movement, 2) conducting a cross sectional analysis of the impact (reduction of outbreak size and economic cost) of implementing a national standstill/movement control, 3) use of case specific studies to estimate impact of state/territory level implementation of standstill, and 4) cost analysis and comparison of national standstill with the state/jurisdictional level and risk-based approach …

Sdsu Beef Day 2022 Complete Proceedings, Department Of Animal Science, Sdsu Extension

SDSU Beef Day 2022 Summary Publication

This is the complete proceedings of the South Dakota State University, Department of Animal Science Beef Day 2022. The proceedings contain research presented on the following topics:
Feedlot
Herd Management
Meat Science and Nutrition

Impact Of Corn Silage Moisture And/Or Kernel Processing At Harvest On Finishing Steer Growth Performance, Efficiency Of Dietary Net Energy Utilization And Carcass Traits, Thomas G. Hamilton, Warren Rusche, Julie Walker, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Red Angus steers (n = 192; initial shrunk BW = 983 ± 62.3 lbs) were used in the 112 d finishing experiment at the Southeast Research Farm (SERF) of the South Dakota Agricultural Experiment Station in Beresford. Steers were from a single source and obtained from a local SD auction facility. Steers were received 2 weeks prior to trial initiation. Steers were offered a common diet containing 60% concentrate upon arrival. Steers were transitioned to a 90% concentrate diet over the course of 14 d. Steers were consuming the finishing diet (Table 1) at the initiation of the …

Evaluation Of Dietary Roughage Inclusion In A Single Or Two-Diet System For Backgrounding And Finishing, Thomas G. Hamilton, Warren Rusche, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Pre-conditioned crossbred beef steers (n = 46; initial shrunk [4%] BW = 621 ± 89.1 lbs) were used in a 210-d grow-finish experiment at the Ruminant Nutrition Center (RNC) in Brookings, SD. Steers were fed once daily, and bunks were managed according to a slick bunk management system. Cattle were fed in 25 × 25 ft concrete surface pens (n = 10 pens; 5 pens/treatment) with 25 linear ft of bunk space and heated, concrete, continuous flow waterers. Treatments included: 1) A single-diet program (targeted a 59 Mcal/cwt NEg diet fed for 210-d; 1D) or 2) two-diet program …

Dose Effects Of Encapsulated Butyric Acid And Zinc On Beef Feedlot Steer Growth Performance, Carcass Characteristics, And Dietary Net Energy Utilization, Forest L. Francis, Erin R. Gubbels, Thomas G. Hamilton, Warren Rusche, Doug Lafleur, Jerilyn E. Hergenreder, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Steers (n = 272; shrunk BW = 794 ± 163 pounds) were assigned to one of four dietary treatments in a 143.5 d feedlot finishing trial: 0 g BPZ/ kg diet dry matter (DM) (CON), 1 g BPZ/ kg diet DM (1BPZ), 2 g BPZ/ kg diet DM (2BPZ), or 3 g BPZ/ kg diet DM (3BPZ). Carcass data and liver health outcomes were collected, and feedlot growth performance data and efficiency of dietary net energy utilization were calculated on a carcass-adjusted basis.

Evaluation Of A Direct Fed Microbial And/Or An Enzymatically Hydrolyzed Yeast Product In Diets Containing Monensin Sodium On Feedlot Phase Growth Performance, Efficiency Of Dietary Net Energy Utilization, And Carcass Characteristics In Newly Weaned Beef Steers Fed In Confinement For 258 Days, Erin Gubbels, Forest Francis, Thomas G. Hamilton, Jason Griffin, Warren Rusche, Tom Rehberger, Elliot Block, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Single-sourced, newly weaned steers (n = 256; initial body weight (BW) = 542 ± 3.7 lb) were allotted to 32 pens (n = 8 pens/treatment with 8 steers/pen). Steers were blocked by location in a 2x2 factorial treatment arrangement of DFM (Certillus CP B1801 Dry; Bacillus subtilis, Lactobacillus plantarum; 28 g/steer·d-1) and YCW (Celmanax; 18 g/steer·d-1). Steers were vaccinated and poured at processing and individually weighed on d 1, 14, 42 (end of receiving phase; implanted), 77, 105 (end of growing phase), 133, 161 (implanted), 182, 230 (start ractopamine HCl) and 258. Growth performance and carcass measurements were …

Evaluation Of A Direct Fed Microbial An An Enzymatically Hydrolyzed Yeast Product Fed Alone Or In Combination To Beef Steers Administered Ractopamine Hydrochloride 28 Days Prior To Harvest During Summer Months In The Northern Plains, Erin Gubbels, Forest Francis, Thomas G. Hamilton, Jason Griffin, Warren Rusche, Tom Rehberger, Elliot Block, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Single-sourced, newly weaned steers (n=256; initial BW=542 ± 3.7lb; n=64 steers/treatment; 8 steers/pen) were blocked by location in a 2×2 factorial arrangement of DFM (Certillus CP B1801 Dry; Bacillus subtilis, Lactobacillus plantarum; 28 g/steer·d-1) and YCW (Celmanax; 18 g/steer·d-1). Temperature-humidity index (THI) was calculated as: THI=0.81×ambient temperature+[relative humidity×(ambient temperature-14.40)]+46.40. On d-1 and 2 and d-21 and 22 on RH, respiration rate (RR) and panting scores (PS) were determined before and after AM and PM feedings (0700h, 1100h, 1400h, 1700h). RR (n=3 steers/pen) was calculated from: 600/seconds required for 10 flank movements. PS utilized this scoring system: 0 (not …

Effects Of On-Arrival Application Of A Modified-Live Respiratory And Clostridia Vaccination On Health, Growth Performance, And Antibody Titers Of Newly-Weaned Calves, Erin Gubbels, Santana Hanson, Tommy Norman, Thiago Ribeiro, Thomas G. Hamilton, Forest Francis, Jason Griffin, Warren Rusche, Zachary K. F. Smith

SDSU Beef Day 2022 Summary Publication

Study Description:

Single-sourced, newly weaned steers (n=70; initial body weight (BW)=560±12.9lb) were allotted to 10 pens (n=5 pens/treatment with 7 steers/pen). Steers were blocked by BW in a randomized complete block design of VAC [vaccinated for IBR, BVD 1 and 2, PI3, and BRSV (Bovi-Shield Gold 5, Zoetis, Parsippany, NJ) and clostridial (Ultrabec 7/Somubac, Zoetis) upon arrival] or NOVAC (not vaccinated for IBR, BVD 1 and 2, PI3, and BRSV or clostridial species upon arrival). Steers were individually weighed on d 0 (arrival), 1, 21, and 42 for growth performance measures. Whole blood samples (10 mL) were collected (n=3 steers/pen …

Principles Of Holistic Resource Management: Investing In Self-Education, Pete Bauman

SDSU Beef Day 2022 Summary Publication

Study Description:

First developed as a concept for approaching grazing resources management by Alan Savory in the 1970’s as a means of combating poor grazing practices, the concept of Holistic Management has evolved in the modern era to Holistic Resource Management, or in some cases “Wholistic” Resource Management, suggesting a more inclusive approach to overall planning and assessment beyond developing a sustainable grazing plan.

In simplified terms, a complete Holistic Resource Management plan for an agricultural businesses ultimately embraces a core concept that centers on the original ‘Triple Bottom Line’ accounting philosophy where the three “P’s” of profit, people, and …

Use Of Pregnancy Associated Glycoproteins To Determine Fetal Age Throughout Gestation And Clearance Rate In Postpartum Beef Cattle, Adalaide C. Kline, Julie Walker, Taylor N. Andrews, Saulo Menegatti Zoca, Kaitlin M. Epperson, Lacey K. Quail, Jerica J.J. Rich, Jim R. Rhoades, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Previously identified pregnant females from four different herds and postpartum females from one herd were utilized. Blood samples were collected (n = 1,753; study 1) between d 28 and 285 of gestation and (heifers n = 418 and cows n = 657; study 2) once a week for up to 12 weeks after calving. Serum was tested in duplicate using a commercially available blood pregnancy test, IDEXX Alertys Pregnancy Test. In study 1, procedures were modified to allow PAG concentrations to fall within the detectible range of the assay. Data were analyzed using the MIXED procedure of SAS …

Comparison Of Lateral Flow To Other Pregnancy Determination Methods In Order To Determine Accuracy Of Pregnancy Status In Beef Cattle Pre And Postpartum, Adalaide C. Kline, Julie Walker, Taylor N. Andrews, Saulo Menegatti Zoca, Kaitlin M. Epperson, Lacey K. Quail, Jaclyn N. Ketchum, Jerica J.J. Rich, Jim R. Rhoades, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Blood samples were collected from six different Bos taurus herds between day 27 and 285 of gestation (heifers n = 1,205 and cows n = 1,539). Blood samples to determine PAG clearance interval were collected weekly postpartum for up to 12 weeks (heifers n = 418 and cows n = 657). Serum was tested using the lateral flow test and were read by two technicians who were blind to pregnancy status. Level of agreement between the tests were determined by Pearson’s correlation coefficients and Kappa scores. The MIXED procedure of SAS was used to evaluate the effect of …

Use Of Sperm In Vitro Capacitation And Flow Cytometry To Estimate Bull Fertility, Saulo Menegatti Zoca, Thomas W. Geary, Abigail L. Zezeski, Karl C. Kerns, Joseph C. Dalton, Bo R. Harstine, Matthew D. Utt, Robert A. Cushman, Julie Walker, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Frozen-thawed semen from five bulls previously identified as high (48.1% and 47.7%, bulls A and B, respectively), intermediary (45.5%, bull C) or low (43.1% and 40.7%, bulls D and E, respectively) fertility, based on pregnancy per AI, were evaluated with several laboratory measures. Measures included total motility, sperm plasma membrane integrity (viability), acrosome integrity, reactive oxygen species (ROS), mitochondrial membrane energy potential (mito-potential), zinc signatures (signatures 1 to 4), and CD9 protein populations at pre-wash, post-wash, h 0 (diluted with non-capacitation media), and at 0, 3, 6, and 24 h after dilution with capacitation media and incubation at …

Relationship Of Dag1 And Serpina5 Sperm Proteins With Bull Fertility, Saulo Menegatti Zoca, Julie Walker, Jerica J. J. Rich, Kaitlin M. Epperson, Taylor N. Andrews, Adalaide C. Kline, Jessica Nora Drum, M. Sofia Ortega, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Semen from 22 dairy bulls was used to evaluate the presence, localization, and quantification of DAG1 and SERPINA5 on sperm. Sperm motility parameters and viability was also evaluated for semen from each bull. Semen from 19 out of the 22 dairy bulls was used for in vitro embryo production (two Low-SCR and one High-SCR were not available for in vitro embryo production). Bulls were classified based on their sire conception rates (SCR) values as High-SCR (SCR > 1.0) or Low-SCR fertility (SCR < -4.0). Low fertility bulls were subdivided based on their blastocyst rate (BL) as High-BL (Low-SCR/High-BL BL ≥ 31%) or Low-BL (Low-SCR/Low-BL BL ≤ 26%), and High-SCR bulls were not subdivided. The GLM procedure in SAS was used with bull as a fixed effect to determine if variance was greater between bulls compared to within bull. Correlations were determined among DAG1 and SERPINA5 concentrations, percentage of tail labeled for SERPINA5, SCR, sperm total motility, progressive motility, and viability, and in vitro embryo produced cleavage rate (CL) and BL. The GLIMMIX procedure of SAS was used to evaluate the relationship of bull field fertility (High- and Low-SCR), and field and in vitro fertility (High-SCR, Low-SCR/High-BL, Low-SCR/Low-BL) classifications with sperm total (TMOT) and progressive (PROG) motility, viability, CL, BL, DAG1 and SERPINA5 relative concentration, and proportion of sperm tail labeled for SERPINA5. Both SERPINA5 and DAG1 were localized on the sperm head; however, SERPINA5 was also localized on the sperm tail. There was greater variance in concentration among bulls compared to within bull for both DAG1 (P < 0.01; 69.4 vs 49.1, respectively) and SERPINA5 (P < 0.01; 325.8 vs 285.4, respectively). There was a positive correlation between concentration of DAG1 and SERPINA5 (P = 0.01; r = 0.54). Concentrations of SERPINA5 were also correlated with CL (P = 0.04; r = 0.48), and percentage of sperm tail labeled for SERPINA5 was correlated with viability (P = 0.05; r = 0.44) and tended to be correlated with CL (P = 0.10; r = 0.39). There was no relationship between SCR or BL rate classifications and DAG1 (P ≥ 0.66), SERPINA5 (P ≥ 0.54), or percentage of sperm tail labeled for SERPINA5 (P ≥ 0.48).

Proteomic Analysis Of Epididymal And Ejaculated Sperm And Respective Fluids, Saulo Menegatti Zoca, Emmalee J. Northrop-Albrecht, Julie Walker, Robert A. Cushman, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Ejaculated and epididymal semen was collected from mature Angus bulls (n = 9), and then centrifuged to separate sperm and fluid. Fluids were collected and sperm pellets were resuspended in a high ionic solution and vortexed to remove loosely attached proteins. Sperm samples were centrifuged, and the supernatant was collected. Samples collected for protein analysis were snap frozen in liquid nitrogen and stored at -80 ºC. Protein analysis was performed by liquid chromatography with tandem mass spectrometry (LCMS/MS). Yearling Angus cross bulls (n = 40) were used for sperm cultures. Ejaculated (n = 20) and epididymal (n = …

Effect Of Estrous Synchronization With Natural Service Or Fixed-Timed Artificial Insemination Using Conventional Or Gender-Kkewed Semen In Beef Females On Calving Distribution And Post Weaning Calf Performance, Julie Walker, Jerica J.J. Rich, Warren Rusche, Matthew Diersen, George Perry

SDSU Beef Day 2022 Summary Publication

Study Description:

Within 10 herds, beef females (n = 1,620) were either: 1) not synchronized (NonSyn) and mated to bulls, 2) synchronized (7-d controlled internal drug release (CIDR)) and mated to bulls (SynNS) 3) synchronized (7-d CO-Synch plus CIDR) and artificially inseminated with conventional semen (SynAI), or 4) synchronized (7-d CO-Synch plus CIDR) and artificially inseminated with SEXED semen. Calving distributions (calves born from d 1 to 14, 1 to 21, 22 to 42, and 43 and greater) were determined by actual birthdates and calf gender was determined at birth. Over a two-year period, a subset of calves (n = …

Improving Heifer Development Programs Using Precision Technology And Ddgs, Anna Dagel, Hector Menendez Iii, Jameson R. Brennan

SDSU Beef Day 2022 Summary Publication

Study Description:

Heifers were stratified into two groups based on initial body weight and each group was randomly assigned to one of two treatments: a control group (n=30) and a precision group (n=30). Treatment groups are separated into different pastures to graze dormant native range from November to April. Groups are rotated monthly to reduce individual pasture influence. Heifers in the control group are supplemented as a group and bunk fed 5lb/head/day with dried distiller grain pellets (DDGS). Heifers within the precision group will be supplemented individually using the SmartFeed system (C-Lock Inc. Rapid City, SD) and offered 5lb/head/day DDGS …

Factors Influencing Price Of South Dakota Feeder Calves Fall 2021 And Winter 2022, Adele Harty, Olivia Amundson, Robin Salverson, Kiernan Brandt, Heather Gessner, Julie Walker, Hossein Moradi Rekabdarkolaee

SDSU Beef Day 2022 Summary Publication

Study Description:

Data was collected at Faith, Philip, Hub City (Aberdeen), Mitchell, and Ft. Pierre Livestock Auctions during the weeks of October 4, 11, 18, and November 1, 2021. Data was collected and analyzed on the approximately 116,000 head of calves that were marketed during that time frame. Data collected included: auction barn, date, seller id, sex, number of head per lot, average weight, hide color, uniformity information, vaccination data, creep feed, implants, branded programs, weaned, horns, mud score, price per hundredweight, price per head, and additional comments. Uniformity score and weighted average price per hundredweight was calculated for each …

Comparing The Impact Of Continuous And Virtually Fenced Rotational Grazing On Animal Behavior And Distribution, Logan Vandermark, Jameson Brennan, Krista Ehlert, Hector Menendez Iii

SDSU Beef Day 2022 Summary Publication

Study Description:

Six herds of yearling steers (n=127) will graze native summer pastures from May to August at the Cottonwood Field Station near Philip, South Dakota. Six pastures are divided into two groups: a continuous grazing (CG) treatment with a low, moderate, and high stocking density and a virtually fenced rotational (VFR) grazing system with the same stocking densities. VenceTM virtual fence collars were placed on all animals within the study and collected GPS data at 5-minute intervals. The CG collars are not actively managed. The VFR system is actively managed through the collars and rotated based on the amount …