Dairy Science Commons^™

Altered Gene Response To Aflatoxin B1 In The Spleens Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

Susceptibility and/or resistance to aflatoxin B₁ (AFB₁) is a threshold trait governed principally by glutathione S transferase (GST)-mediated detoxification. In poultry, domesticated turkeys are highly sensitive to AFB₁, most likely due to dysfunction in hepatic GSTs. In contrast, wild turkeys are comparatively resistant to aflatoxicosis due to the presence of functional hepatic GSTAs and other possible physiological and immunological interactions. The underlying genetic basis for the disparate GST function in turkeys is unknown as are the broader molecular interactions that control the systemic response. This study quantifies the effects of dietary AFB₁ on gene …

Efficacy Of A Ml336 Derivative Against Venezuelan And Eastern Equine Encephalitis Viruses, Colleen B. Jonsson, Xufeng Cao, Jasper Lee, Jon D. Gabbard, Yong-Kyu Chu, Elizabeth A. Fitzpatrick, Justin G. Julander, Dong-Hoon Chung, Jennifer Stabenow, Jennifer E. Golden

Animal, Dairy, and Veterinary Science Faculty Publications

Currently, there are no licensed human vaccines or antivirals for treatment of or prevention from infection with encephalitic alphaviruses. Because epidemics are sporadic and unpredictable, and endemic disease is common but rarely diagnosed, it is difficult to identify all populations requiring vaccination; thus, an effective post-exposure treatment method is needed to interrupt ongoing outbreaks. To address this public health need, we have continued development of ML336 to deliver a molecule with prophylactic and therapeutic potential that could be relevant for use in natural epidemics or deliberate release scenario for Venezuelan equine encephalitis virus (VEEV). We report findings from in vitro …

Quantification Of Tylosin Antibiotics In Cattle Waste, Appala Keerthi

Masters Theses & Specialist Projects

Antibiotics are used as prophylactic agents to promote growth and for treating infections in animals. However, the irrational use of antibiotics in livestock management is a significant cause of the development of antibioticresistant genes in the environment. Each year 2 million people suffer from the infections caused by bacteria which are resistant to antibiotics and 23,000 of these people are estimated to die because of antibiotic resistance. New drugs are continually coming into the market but are at the risk of developing resistance. Thus, there is a need for the development of analytical methods which can be used to monitor …

Islands In A Sea Of Aaruul: Globalization And Mongolian Cheesemakers, Pearse Anderson

Independent Study Project (ISP) Collection

Despite being a sparsely-populated country with less than 2% of its land arable, Mongolia has developed a rich and complex food culture, notably for its meat and dairy products, which could soon be at an historic high thanks to the tens of millions of animals on Mongolian pastureland. Many Mongolians and non-Mongolians view the countryside as a sea of milk that is currently being underutilized for economic exploitation. Various projects, whether funded and organized by international NGOs, the Mongolian government, or private companies, have tried to use Mongolian’s dairy resources to fill Mongolian demand, with more recent private ventures also …

Evaluating The Impact Of Feed Supplementation On Productive And Reproductive Efficiency In Smallholder Dairy Cattle In Arusha, Tanzania, Hayley Hall

Independent Study Project (ISP) Collection

This study was performed to determine if supplementing concentrates and/or minerals significantly affect productive and reproductive efficiency in smallholder dairy cattle in Arusha, Tanzania. The goal was to estimate the necessity and effectiveness of supplementing a forage diet on production and reproduction. Data was collected through interviews of small scale dairy cattle owners in nine regions within and around Arusha. Productive efficiency was measured by milk yield per cow per day. Reproductive efficiency was evaluated by the amount of time to heat resumption after calving and reproductive disease status. This was studied because determining the factors that improve the efficiency …

Mapping Of Dynamic Transcriptome Changes Associated With Silica-Triggered Autoimmune Pathogenesis In The Lupus-Prone Nzbwf1 Mouse, Melissa A. Bates, Abby D. Benninghoff, Kristen N. Gilley, Andrij Holian, Jack R. Harkema, James J. Pestka

Animal, Dairy, and Veterinary Science Faculty Publications

Crystalline silica (cSiO₂) is a widely recognized environmental trigger of autoimmune disease. In the lupus-prone female NZBWF1 mouse, airway exposure to cSiO₂ triggers pulmonary ectopic lymphoid neogenesis, systemic autoantibody elevation, and glomerulonephritis. Here we tested the hypothesis that upregulation of adaptive immune function genes in the lung precedes cSiO₂-triggering of autoimmune disease in this model. The study include three groups of mice, as follows: (1) necropsied 1 d after a single intranasal instillation of 1 mg cSiO₂ or vehicle, (2) necropsied 1 d after four weekly single instillations of 1 mg cSiO₂ or …

Fermentation Of Prebiotics In Whole Food Powders By Probiotic Bacteria Strains, Michaela Brubaker, Sumira Phatak

Research on Capitol Hill

Colorectal cancer (CRC) is the second leading cause of cancer death in the United States. Modification of the gut microbiome offers potential for CRC prevention.

Generation Of H7n9-Specific Human Polyclonal Antibodies From A Transchromosomic Goat (Caprine) System, Hua Wu, Zhiqiang Fan, Michelle Brandsrud, Qinggang Meng, Molly Bobbitt, Misha Regouski, Rusty Stott, Alexis Sweat, Jackelyn Crabtree, Robert J. Hogan, Ralph A. Tripp, Zhongde Wang, Irina A. Polejaeva, Eddie J. Sullivan

Animal, Dairy, and Veterinary Science Faculty Publications

To address the unmet needs for human polyclonal antibodies both as therapeutics and diagnostic reagents, building upon our previously established transchromosomic (Tc) cattle platform, we report herein the development of a Tc goat system expressing human polyclonal antibodies in their sera. In the Tc goat system, a human artificial chromosome (HAC) comprising the entire human immunoglobulin (Ig) gene repertoire in the germline configuration was introduced into the genetic makeup of the domestic goat. We achieved this by transferring the HAC into goat fetal fibroblast cells followed by somatic cell nuclear transfer for Tc goat production. Gene and protein expression analyses …

Human Polyclonal Antibodies Produced From Transchromosomal Bovine Provides Prophylactic And Therapeutic Protections Against Zika Virus Infection In Stat2 Ko Syrian Hamsters, Venkatraman Siddharthan, Jinxin Miao, Arnaud J. Van Wettere, Rong Li, Hua Wu, Eddie Sullivan, Jinan Jiao, Jay W. Hooper, David Safronetz, John D. Morrey, Justin G. Julander, Zhongde Wang

Animal, Dairy, and Veterinary Science Faculty Publications

Zika virus (ZIKV) infection can cause severe congenital diseases, such as microcephaly, ocular defects and arthrogryposis in fetuses, and Guillain–Barré syndrome in adults. Efficacious therapeutic treatments for infected patients, as well as prophylactic treatments to prevent new infections are needed for combating ZIKV infection. Here, we report that ZIKV-specific human polyclonal antibodies (SAB-155), elicited in transchromosomal bovine (TcB), provide significant protection from infection by ZIKV in STAT2 knockout (KO) golden Syrian hamsters both prophylactically and therapeutically. These antibodies also prevent testicular lesions in this hamster model. Our data indicate that antibody-mediated immunotherapy is effective in treating ZIKV infection. Because suitable …

Differential Transcriptome Responses To Aflatoxin B1 In The Cecal Tonsil Of Susceptible And Resistant Turkeys, Kent M. Reed, Kristelle M. Mendoza, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

The nearly-ubiquitous food and feed-borne mycotoxin aflatoxin B₁ (AFB₁) is carcinogenic and mutagenic, posing a food safety threat to humans and animals. One of the most susceptible animal species known and thus a good model for characterizing toxicological pathways, is the domesticated turkey (DT), a condition likely due, at least in part, to deficient hepatic AFB₁-detoxifying alpha-class glutathione S-transferases (GSTAs). Conversely, wild turkeys (Eastern wild, EW) are relatively resistant to the hepatotoxic, hepatocarcinogenic and immunosuppressive effects of AFB₁ owing to functional gene expression and presence of functional hepatic GSTAs. This study was designed to …

Jackrabbit Dairy And Food Review, Vikram V. Mistry

The Jackrabbit Dairy & Food Review

This is the Winter 2017- 2018 Jackrabbit Dairy and Food Review. It contains the following articles and information: Message from the Department Head, 2017 Graduates, David A. Thompson Endowment, New Faculty and Visitors, information on the Dairy Club and Food Science Club, Judging Activities, Student Accomplishments, Professional Activities of Faculty and Staff, Distinguished Alumni, Dairy Facilities, a list of Scholarships and Dairy and Food Science Students, information on Departmental Outreach and Research Highlights.

Perceptions Of On-Farm Emergency Slaughter For Dairy Cows In British Columbia, Katherine E. Koralesky, David Fraser

Slaughter and Slaughtering Practices Collection

Some jurisdictions permit on-farm emergency slaughter (OFES) as one end-of-life option for dairy cows and other animals that cannot be transported humanely but are deemed fit for human consumption. Anecdotal reports suggest that OFES is controversial among dairy industry professionals, but to date their perceptions of OFES have not been studied systematically. Twentyfive individual interviews and 3 focus groups with 40 dairy producers, veterinarians, and other professionals in British Columbia, Canada, revealed positive and negative perceptions of OFES influenced by (1) individual values, (2) the perceived operational legitimacy of OFES, and (3) concern over social responsibility and public perception of …

Using An Implantable Microchip For Measuring Body Temperature In Dairy Calves, Megan Woodrum, Melissa Cantor, Joao H. C. Costa

Posters-at-the-Capitol Presentations

Body temperature is frequently used as a method for determining if illness is present with fever detection. Taking rectal temperature or alternatively tympanic temperature can be time consuming and requires restraint of calves. Alternatives, such as implantable microchips that can be passively read using a radio frequency identification (RFID) scanner may allow for easier monitoring of body temperature.

This study aim was to validate an implantable microchip and to determine the best implant site.

Modelling And Simulation Of Renewable Technologies For Economic And Environmental Analysis And Optimization Of Dairy Farms, Michael Christopher Breen

Theses

The aim of this thesis was to develop a dairy optimisation platform to optimise investments in dairy farm technology and changes to on-farm management practices and electricity tariffs, in order to maximise profitability and minimise environmental impacts. Increased dairy herd sizes and milk production levels in Ireland as well as changing regulations concerning emissions, renewable penetration and energy efficiency have necessitated a means of financial decision support for farmers. Therefore this body of work focused on the creation of a comprehensive method for optimising dairy farm technology selection, management practices and electricity tariffs. The measures which could be implemented on …

Hydrodynamic Cavitation As In-Line Process To Control Common Dairy Sporeformers, Pratibha Chaudhary

Electronic Theses and Dissertations

Sporeforming bacteria and their endospores have the ability to survive thermal treatments and proliferate in the milk and dairy products to cause spoilages. We applied the effect of hydrodynamic cavitation in controlling these bacteria and their spores at pilot scale level. Our aim was to investigate a technology that would improve the microbial quality of milk and dairy products, and is capable of being scaled up to be adapted at industrial level. Cavitation damages the cell walls of the bacteria and their endospores (releasing dipicolinic acid, responsible for endospore resistance to extreme conditions), thereby making them susceptible to thermal treatments …

Evaluation Of Feeding Alternative Feedstuffs Including Hydroponic Barley Sprouts And Carinata Meal To Dairy Cattle, Rhea D. Lawrence

Electronic Theses and Dissertations

The purpose of this dissertation research was to examine alternative feed ingredients not typically found in dairy cattle diets. In total, four studies were conducted to evaluate feedstuffs such as hydroponic barley sprouts and carinata meal and how they affect cattle performance. To determine how feeding hydroponic barley sprouts would affect growing dairy heifer and lactating cow performance two feeding studies were conducted. In the first study, inclusion of 14 % (DM basis) hydroponic barley sprouts (HYD) was evaluated in an ad libitum total mixed ration (TMR) compared to a control (CON) diet on dairy heifer performance during a 12 …

Evaluation Of Effects Of Methods Used For Forage Analysis And Dietary Inclusion Of Buffer On Fiber Digestibility And Rumen Fermentation In Diets High In Distillers Grains, Lydia Kehinde Olagunju

Electronic Theses and Dissertations

Forage quality is an important factor affecting intake and utilization of forage, thus making it imperative to evaluate the methods used to determine the nutritive values of forage in order to predict animal performance. Additionally, fibrous feeds for ruminants are less subject to competitive demand. The plant cell wall is the largest hindrance to complete digestion of feeds, particularly forages and by-products and to the utilization of the nutrients and energy they contain, necessitating effective strategies for increasing the rate and efficiency of utilization of forage fiber and the energy therein. It is critically important to increase fiber digestion for …

Evaluation Of Flax Oil Supplementation On Performance Of Growing Dairy Heifer Calves And Lactating Cows, Chelsea Rose Schossow

Electronic Theses and Dissertations

Utilization of flax oil is new to the livestock industry, and limited research has been done to see the benefits of feeding the highly unsaturated oil to both dairy calves and lactating cows. Two studies were conducted to evaluate the potential benefits of flax oil as a lipid supplement. The first study compared the supplementation of two plant based oils to growing dairy calves. Thirty-six female Holstein calves in individual hutches were used in a 12-wk randomized complete block design study. Treatments were: 1) control (CON) with no oil, 2) 80 g/d of flax oil (FLAX), and 3) and 80 …

Assessing Listeria Risk During Different Stages Of Ice Cream Manufacturing And Storage, Neha Singh

Electronic Theses and Dissertations

Listeriosis is a life-threatening infection caused by foods contaminated with Listeria monocytogenes. Some of the major ice cream recalls in recent years reaffirm the ability of this food-borne pathogen to survive in diverse dairy processing environments and cause cross contamination. Inspection reports revealed certain lapses in implementing adequate hygienic practices for Listeria persistence in the processing environment, leading to cross contamination of ice cream. The higher levels of cross contamination of raw ice cream mix might result in random heat-injured cells when exposed to minimum heat treatment (69°C for 30 min). Evidence about the presence of injured cells in ice …

Novel Approaches To Evaluate And Enhance Neonatal Calf Gastrointestinal Health And Development, Fernanda Trindade Da Rosa

Electronic Theses and Dissertations

Physiological adaptations of the gastrointestinal tract (GIT) epithelial cells as well as methods to manipulate early life GIT microbiota colonization during the neonatal stage, is of great importance to the dairy industry. The first objective of this research was to optimize a method based on evaluation of bovine transcripts in fecal RNA via RTqPCR using L-selectin (SELL) as a marker for polymorphonuclear leukocytes (PMNL), keratin 8 (KRT8) and fatty acid binding protein 2 (FABP2) for GIT enterocytes, and tight junctions in fecal RNA and GIT sections in dairy calves. To test the reliability of the fecal RNA method, fecal and …

Effects Of Yeast-Derived Microbial Protein On Transition Dairy Cow Health And Performance, Gustavo Mazon Correa Alves

Theses and Dissertations--Animal and Food Sciences

The transition period for dairy cows is defined as the three weeks pre and postpartum. During the transition period, dairy cows experience a myriad of metabolic, managerial, and nutritional requirement changes. These changes lead to stress and increased susceptibility to diseases which can negatively affect lactational performance in the short and long term. However, dietary amino acid availability can have a dramatic impact on the health and performance of dairy cows around parturition. Thus, the objective of the thesis was to evaluate the effects of supplementing yeast-derived microbial protein, as an alternative protein source for dairy cows during the transition …

Evaluating Costs Associated With Management Decisions Of Replacement Dairy Heifers And Their Impact On The Total Rearing Investment, Anna Catherine Hawkins

Theses and Dissertations--Animal and Food Sciences

Replacement heifer rearing is critical for the future of the dairy operation, especially to improve genetic merit and maintain herd size. A replacement heifer from the day she is born to the day she calves herself is generally a 2-year investment without potential income. A myriad of options exists on how to manage, fed, and ultimately raise replacement heifers. This study quantifies the costs associated with replacement heifer management decisions from birth to calving related to housing, labor, feed and health. The heifer rearing period can be broken into pre and post weaning sections to allow for more understanding the …

Growth Performance, Metabolic And Rumen Profile, Nutrient Utilization, And Health Of Calves Fed Condensed Whey Solubles With Starter Pellets, Michaela Joy Della

Electronic Theses and Dissertations

A study was conducted for this thesis to evaluate the effectiveness of a new product’s ability to improve calf health and growth performance. In addition to the results from the study, this thesis also includes a literature review of immune and rumen maturation in calves and a discussion of ways to protect and encourage those fundamental progressions. For the main research project 48 dairy calves were used in a randomized complete block design study to evaluate the growth performance, rumen fermentation and metabolic profiles, nutrient utilization, and health scores when calves were supplemented Condensed Whey Solubles (CWS) with their starter …

Using Isoconversional Methods To Study The Effect Of Antioxidants On The Oxidation Kinetics Of Milk Fat, Khalid Ahmed Alsaleem

Electronic Theses and Dissertations

Milk fat is a versatile ingredient because of its nutritional value, functionality, and flavor. During processing and storage, milk fat may undergo oxidation resulting in many undesirable changes such as unpleasant flavor and aroma, and formation of toxic compounds. A common practice to prevent the oxidation of milk fat is by the addition of antioxidants. It is worth to mention that little is known on the effect of antioxidants on the oxidation kinetics. In this work, the effect of selected antioxidants on the oxidation kinetics of anhydrous milk fat (AMF) under non-isothermal conditions was investigated. AMF with an addition of …

Automated Body Condition Scoring: Progression Across Lactation And Its Association With Disease And Reproduction In Dairy Cattle, Carissa Marie Truman

Theses and Dissertations--Animal and Food Sciences

Body condition scoring is a technique used to noninvasively assess fat reserves. It provides an objective estimate to describe the current and past nutritional status of the dairy cow and has been associated with increased disease risk and breeding success. Traditionally body condition scores are taken manually by visual appraisal on a 1 to 5 scale, in one-quarter increments. However, recent studies have shown the potential of automating the body condition scoring of cows using images. The first objective was to estimate the likelihood of disease development and breeding success, using odds ratios, associated with body condition score scored automatically …

Validation Of An Automated Behavior Monitoring Collar, And Evaluation Of Heat Stress On Lactating Dairy Cow Behavior With Access To A Free Choice Soaker, Lori Nicole Grinter

Theses and Dissertations--Animal and Food Sciences

Precision dairy technologies (PDT) are becoming more accessible and are therefore becoming more common on commercial dairy farms and in dairy research. Prior to any use of PDT, one should understand the precision, accuracy and bias of the device by a validations studies before interpreting the behavior measurements. Thus, the objective of the first section of my thesis is to validate ruminating, feeding and resting measurements of a behavior monitoring collar used in the second section. Precision dairy technology is used in heat stress studies to compare behavior of cows exposed to different heat stress treatments or abatement strategies. Heat …

The Effect Of Bovine Galectin-1, A Conceptus Secretory Protein, On The Endometrial Transcriptome, Lindsay Faye Grose

Graduate Theses, Dissertations, and Problem Reports (ETD)

Early embryonic loss in cattle is detrimental to reproduction thus, the profitability of both the beef and dairy industries. Therefore, an important aspect of study is to find techniques that beef or dairy producers could use to decrease early embryonic loss Galectin-1, the protein that was investigated in this research is important in modulating the maternal immune system in rodent and human early pregnancies by promoting fetomaternal immune tolerance. The role of galectin-1 within the reproductive tissues has not been thoroughly investigated in cattle. Galectin-1 is located in the caruncular and intercaruncular regions of the bovine endometrium, specifically the maternal …