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Weaving An Interdisciplinary Microbiome Career Using Threads From Different Ecosystems, Sarah Hosler Aug 2022

Weaving An Interdisciplinary Microbiome Career Using Threads From Different Ecosystems, Sarah Hosler

Electronic Theses and Dissertations

Animals have trillions of microorganisms living in or on many body sites, these communities of microorganisms are called microbiomes. Microbiomes are typically host-specific, and a lot of information about the host can be determined from investigating them. Microbiome research has many real-world applications, and this thesis utilizes the One Health perspective, which acknowledges the connection of humans, animals, and environments, and emphasizes the need for collaborative, interdisciplinary research. The first interdisciplinary project is an investigation into the bacteria in wild and cultured Atlantic deep-sea scallop, Placopecten magellanicus larvae. Adults in hatcheries can be induced to spawn, but the last two …


The Incidence Of Staphylococcus Aureus Mastitis In Maine Dairy Cattle, Morgan Belvin May 2022

The Incidence Of Staphylococcus Aureus Mastitis In Maine Dairy Cattle, Morgan Belvin

Honors College

This project investigated the factors surrounding the incidence of Staphylococcus aureus (SA) mastitis in Maine dairy cattle. This type of mastitis is driven by an antibiotic resistant pathogen that is very contagious among cattle, though farmers are often unaware of how common this type of mastitis is and that it could be present in their herds. My hypothesis was that specific farm factors, like milkers performing other tasks during milking, as well as the act of pre-dipping versus not pre-dipping, would explain the increase in SA mastitis. A thirty-seven-question IRB-approved survey was subsequently distributed to farmers in the University of …


Testing The Effect Of Grapefruit Seed Extract (Gfse) On The Mastitsis-Causing Alga, Prototheca, Kendra Janelle Macdonald May 2013

Testing The Effect Of Grapefruit Seed Extract (Gfse) On The Mastitsis-Causing Alga, Prototheca, Kendra Janelle Macdonald

Honors College

Mastitis is an inflammation of the mammary gland that costs the United States dairy industry between $1.7 and $2 billion annually [9]. Approximately 97% of mastitis infections are caused by bacteria, which can be treated with antibiotics [2]. However, mastitis infections can be caused by the alga, Prototheca. Specifically, it is P. zopfii (genotype 2) and P. blaschkeae that have been found in cows with mastitis [7]. There are currently no approved treatments for protothecal mastitis. The following experiments a tested the effects of grapefruit seed extract (GFSE) on P. zopfii (genotype 2) using spectrophotometry and plating techniques. GFSE was …