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Beta-Hydroxybutyrate Alters The Mrna Cytokine Profile From Mouse Macrophages Challenged With Streptococcus Uberis, T. H. Swartz, L. K. Mamedova, B. J. Bradford
Beta-Hydroxybutyrate Alters The Mrna Cytokine Profile From Mouse Macrophages Challenged With Streptococcus Uberis, T. H. Swartz, L. K. Mamedova, B. J. Bradford
Kansas Agricultural Experiment Station Research Reports
The objective of this study was to determine if β-hydroxybutyrate (BHB) altered inflammatory responses in macrophages challenged with a common mastitis pathogen,Streptococcus uberis. Mouse macrophages (RAW 264.7 line) were cultured either in the presence or absence of BHB for 24 h, and then challenged or not withS. uberis. Relative transcript abundance of cell membrane receptors (TLR2 and GPR109a), cytokines (IL-1β, IL-10, TNFα, and TGFβ), and chemokines (CXCL2 and CCL5) were determined using quantitative real-time polymerase chain reaction (qPCR) and normalized against the geometric mean of HPRT and B2M.Streptococcus uberisactivated the macrophages, noted by greater …