Dairy Science Commons^™

A Comparison Of Pyronin Y-Methyl Green Stain And Methylene Blue Stain For Somatic Cell Count In Sheep Milk, Emily Mirek, Stacey O’Donnell

Senior Honors Projects

Somatic cell count is a key method used to evaluate the quality of milk in today’s growing dairy sheep industry. Somatic cells are body cells, primarily the white blood cells, found in a milk sample. If an infection such as mastitis is present, the number of somatic cells in the milk increases (Gonzalo, et al. 1992). Producers routinely perform somatic cell counts on cow and goat milk. For dairy cattle in the United States, the somatic cell count can not exceed 750,000 cells per milliliter, for commercial milk. For dairy goats the accepted limit is 1,000,000 cells per mL of …

The Cattle Industry's Guidelines For The Care And Handling Of Cattle

Nebraska Beef Quality Assurance Program

Cattlemen have long recognized the need to properly care for livestock. Sound animal husbandry practices, based on decades of practical experience and research, are known to impact the wellbeing of cattle, individual animal health and herd productivity. Cattle are produced in very diverse environments and geographic locations in the United States. There is not one specific set of production practices that can be recommended for all cattle producers. Personal experience, training and professional judgment can serve as a valuable resource for providing proper animal care.

Selecting Linear-Score Distributions For Modeling Milk-Culture Results, H. G. Allore, David J. Wilson, H. N. Erb, P. A. Oltenacu

Animal, Dairy, and Veterinary Science Faculty Publications

The data for this cross-sectional retrospective study are from surveys of 65 dairy-cattle herds in central New York, USA sampled between February, 1993 and March, 1995. The objective was to identify probability distributions of logarithmically transformed somatic-cell counts (linear score) for use in a simulation model of mastitis and milk quality. Probability density functions were estimated using maximum-likelihood estimators for the linear score of individual-cow composite milk samples culture negative and culture positive for the pathogens Streptococcus agalactiae, Streptococcus non-agalactiae, Staphylococcus aureus, and coagulase-negative staphylococci for the complete dataset and by bulk-tank somatic-cell count group (< 500 000, ≥ 500 000 SCC/ml). Based on the rankings of three goodness-of-fit tests (Anderson-Darling, Kolmogorov-Smirnov and x^{2 …}

Acyclic Phosphonomethylether Nucleosideinhibitors Of Respiratory Viruses, Dale L. Barnard, N. Bischofberger, C. U. Kim, J. H. Huffman, R. W. Sidwell, J. P. Dougherty, W. Lew, M. A. Williams, W. Yang

Animal, Dairy, and Veterinary Science Faculty Publications

A series of acyclic phosphonomethylether nucleosides were synthesized and then evaluated for inhibitory activity against respiratory viruses of clinical significance using CPE inhibition, neutral red uptake and virus yield reduction assays. Of the 20 compounds synthesized, none significantly inhibited influenza A or B viruses or respiratory syncytial virus strains A2, Long or 18537; the selective indices (SI) were less than 10. A new compound, GS-2128 (2R, 5R-9-[2,5-dihydro-5-(phosphonomethoxy)-2-furanyl]adenine; D4API), selectively inhibited adenovirus 5 (SI>10) as did GS-0577 (9-(3-hydroxy-2-phosphonylmethoxypropyl)-adenine; HPMPA) and GS-0504 [(S)-1-[3-hydroxy-2-(phosphonylmethoxypropyl)]-cytosine; HPMPC]. The 50% effective concentrations (EC50) ranged from 8-100 μg mL-1 and 50% cell inhibitory concentrations (CC50) from …

Bovine Mastitis Pathogens In New York Andpennsylvania: Prevalence And Effects On Somatic Cell Count And Milk Production, David J. Wilson, R. N. Gonzalez, H. H. Das

Animal, Dairy, and Veterinary Science Faculty Publications

Milk samples were collected from 108,312 dairy cows during 1601 farm visits made between January 1991 and June 1995. The herd visits were made by personnel from the Central Laboratory of the Quality Milk Promotion Services at Cornell University (Ithaca, NY) to farms located in central New York and northern Pennsylvania. Dairy Herd Improvement Association records were available for 32,978 cows in 327 herds. Intramammary infections, as defined by positive milk cultures, were present in 48.5% of all cows and in 36.3% of cows in herds enrolled in the Dairy Herd Improvement Association. Over 75% of the intramammary infections were …

Comparative Study Of Elongated Chromosomes In Sheep And Goats And A Proposed Standard, Stephen H. Mensher

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A cell synchronization technique was used that consistently yielded numerous elongated chromosomes, which are necessary for producing high-resolution G-banding. The prometaphase stage of contraction was determined to have the greatest utility for detailed comparative analysis and, therefore, was used in the preparation of the proposed standard high-resolution karyotypes and idiogram.

A proposed standard high-resolution G-band karyotype of the domestic sheep and goat is presented, along with a high-resolution G-band idiogram of the goat. The karyotypes presented contain considerably more bands than contemporary karyotypes and provide much greater resolution for linear differentiation. The X chromosome of the sheep is acrocentric, rather …

Herd Improvement Service Forges Ahead, M P. Bond

Journal of the Department of Agriculture, Western Australia, Series 4

Since its establishment three years ago, the Herd Improvement Service has steadily expanded the range of services provided to Western Australian livestock producers. The genetic advancement and improved hers management offered by the service give farmers the ability to use modern technology in the most cost-effective way. There is an increasing realisation amongst farmers that to survive and remain competitive, it is worthwhile to invest in such technology.

The Herd Improvement Service is a statutory corporation which was formed to improve the productivity of the State's livestock enterprises. Most of the organisation's activities have been directed to servicing the dairy …

The Incidence Of Cryptosporidial Infections In Nebraska Dairy Calves, Douglas Lee Varner

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Within the last decade a new etiologic agent has been identified as a possible cause of diarrhea in humans and domestic animals—that being the protozoan parasite Cryptosporidium. The purpose of this study was to determine the incidence and distribution of Cryptosporidium in the state of Nebraska, to determine if Cryptosporidium occurs more frequently in association with other enteropathogens and to determine about the relation of scouring in the calves to infection with Cryptosporidium. A total of 620 fecal samples from 334 dairy calves from 71 herds in Nebraska were examined for cyptosporidial oocysts using the Sheather’s sugar flotation …

Detailed Description And Nomenclature Of High Resolution G-Banded Horse Chromosomes And Cytogenetic Analysis Of Hereditary Multiple Exostosis In Horses, Alma Maciulis

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

A reliable technique for obtaining late prophase, prometaphase and greater numbers of metaphase chromosomes is presented. The increased number of mitoses and high resolution chromosomes were obtained using a modification of the typical three-day blood culture technique in which the cell cycle was temporarily blocked at the G₁-s border with methotrexate. A high resolution G-band idiogram is presented using a system similar to the standardization of human chromosomes. The use of this technique and system of nomenclature will facilitate routine analyses of horse chromosomes and provide a basis for the identification and recording of chromosome aberrations in this …

Controlling Annual Ryegrass Toxicity, C H. Trotman

Journal of the Department of Agriculture, Western Australia, Series 4

Eight years of intensive work has changed annual ryegrass toxicity from an unknown killer to a manageable problem.

Cancer Eye Of Cattle, M R. Gardiner, J. L. Anderson, D. E. Robertson

Journal of the Department of Agriculture, Western Australia, Series 4

Cancer of the eye and eyelids is mainly a disease of cattle, although it occasionally occurs in most species of animals and in man. It is the most common cancer in cattle, especially in certain breeds.

Prevent Worms In Cattle, G C De Chaneet

Journal of the Department of Agriculture, Western Australia, Series 4

PARASITIC gastro enteritis, caused by roundworms, is a common cause of economic loss in calves, yearlings and occasionally cows in south-western Australia. Losses could be prevented if stock owners realised the full effects of these parasites, and understood the causes of worm infestation.

Effects Of Mastitis On Milk Yield And Composition, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

EVERY DAIRY farmer knows how much he spends on the treatment of cows affected with mastitis, but the actual loss in production is more difficult to estimate.

As part of the current mastitis research programme the Department of Agriculture's Animal and Dairying Divisions surveyed 37 dairy herds with a believed high incidence of mastitis.

Calf Diarrhea (Scours): Reproduced With A Virus From A Field Outbreak, C. A. Mebus, N. R. Underdahl, M. B. Rhodes, M. J. Twiehaus

Nebraska Agricultural Experiment Station: Historical Research Bulletins

Neonatal calf diarrhea typical of that seen in field cases was produced in colostrum-deprived calves by inoculating either feces or bacteria-free filtrates via the duodenum, orally, or by a spray (aerosol). Three calves, kept free of E. coli, developed severe diarrhea following inoculation with a bacteria-free filtrate. Intense fluorescence in the epithelium of sections of small intestine from two experimental calves was observed following staining with ftuorescein-labeled gamma globulin produced with viral antigen prepared from the feces of two experimental diarrhetic E. coli-free calves. Fluorescing cells were also present in the feces of experimental and field cases of neonatal …

Black-Leg In Cattle, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

BLACK-LEG is still a serious disease of cattle in the South West coastal region, causing deaths in young cattle.

The disease is characterised by hot swollen muscles, acute lameness and rapid death.

Cattle affected are usually 6 to 12 months old, in fat condition, and grazing on lush pastures.

Can Mastitis Be Eliminated?, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

Can mastitis be eliminated from a dairy herd? The results of a 12-month continuous study of four commercial herds show that it could be done.

Bovine Tuberculosis Control In Western Australia : Past, Present And Future, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

TWENTY YEARS AGO, tuberculosis was a widespread and serious disease in the dairy cattle of Western Australia.

Besides having a marked depressing effect on the economy of the dairying industry, bovine tuberculosis was a constant hazard to humans coming in contact with it.

Mastitis In Western Australian Dairy Cattle, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

IN 1964/65, as a first step towards a State-wide mastitis control programme, the Department of Agriculture undertook a survey of mastitis in West Australian dairy herds.

The survey was designed to obtain a clear picture of the incidence and severity of the disease and is the most comprehensive mastitis survey carried out in Australia.

Feeding Urea To Dairy Cows, R A. Bettenay

Journal of the Department of Agriculture, Western Australia, Series 4

IT has been known for some years that the ruminant animal, through the agency of microflora in the rumen, has the ability to use urea as a source of nitrogen, and to convert some of this to protein.

Coccidiosis Of Calves, W A. Halhead

Journal of the Department of Agriculture, Western Australia, Series 4

Coccidiosis is one of the commonest causes of calf loss and debility on South-West dairy farms.

Treatment and control are simple once the nature of the disease is understood.

Animal Husbandry Research At Bramley, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

A progress report of animal husbandry investigations at the Bramley Animal Husbandry Research Station, Margaret River.

BRAMLEY RESEARCH STATION is four miles north of Margaret River.

The average annual rainfall of 45 inches falls mainly in the winter, providing a seven-month growing season which starts in March or April.

The June-July period is excessively wet, causing extensive waterlogging of the soils.

Cow's Hoofs Need To Be Watched, Department Of Agriculture, Western Australia

Journal of the Department of Agriculture, Western Australia, Series 4

THE need for thorough trimming of the hoofs of sheep for the control of foot rot is recognized, but few realize the need for trimming the hoofs of cattle.

Mastitis : Prevention And Control. 3. Implementing The Mastitis Control Programme, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

FARMERS who have followed the first two articles in this series should by now have established the recommended milking routine and be familiar with it.

Calfhood Diseases. 2. Respiratory And Nutritional Deficiency Diseases, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

MOST of the common organisms found in the environment of calf sheds and yards may at times be responsible for respiratory infections, to which the young animal is very susceptible.

Remarkable Success Of Bovine T.B. Eradication Campaign, J Shilkin

Journal of the Department of Agriculture, Western Australia, Series 4

Less than 20 years ago Perth dairy herds had the highest T.B. incidence of any Australian capital city and half of the Kalgoorlie cattle had the disease.

Why this was so and how the eradication campaign has reduced the incidence of less than 0.3 per cent, in 128,000 cattle is discussed in this article.

Calfhood Diseases. 1. Gastro-Intestinal Diseases, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

CALFHOOD disease may be divided for convenience into three main categories:

• Those affecting the gastro-intestinal tract, including diseases of an infectious nature and those arising as a result of infestation by worms (parasitism).

• Those affecting the respiratory system, including lungworms.

• Those resulting from nutritional deficiencies, especially vitamins and minerals.

Mastitis : Its Prevention And Control. 1. The Udder In Health And Disease, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

This is the first of a series of four articles on mastitis which will appear in consecutive issues of the Journal of Agriculture.

This is an introductory article describing the cow's udder in health and disease.

Mastitis : Prevention And Control. 2. Principles Of Mastitis Control, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

MASTITIS is a major cause of financial loss in over half our dairy herds.

Losses result from treatment costs, milk discarded, permanent loss of milk production, culling, lowering of solids-not-fat content and loss of butter-fat production.

Mastitis CAN be controlled. This article outlines the basic principles of mastitis control in the milking shed.

Mastitis : Prevention And Control. 4. The Mastitis Control Programme Continued, F C. Wilkinson

Journal of the Department of Agriculture, Western Australia, Series 4

PREVIOUS articles in this series have described mastitis and the principles of mastitis control, and suggested how a control programme may be established.

This article outlines the routine measures which should be maintained for continuation of the programme.

Internal Parasites Of Cattle, J Craig, R. Butler, M. E. Nairn

Journal of the Department of Agriculture, Western Australia, Series 4

Worm parasites can have a most serious effect on cattle, even causing deaths.

This article describes the main parasites of cattle, treatment with modern anthelmintic drugs and the best means of preventing and controlling worm outbreaks.