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Full-Text Articles in Beef Science

Honeybee Colony Response To Probiotic Supplementation And Grazing Practices, Katya M. Busenitz May 2026

Honeybee Colony Response To Probiotic Supplementation And Grazing Practices, Katya M. Busenitz

Department of Entomology: Dissertations, Theses, and Student Research

Honeybee health is a complicated issue. Studies were conducted to scale honeybee (Apis mellifera) health from the microbiome to the landscape. Colony health parameters were measured in response to probiotic administration. Colony capped brood production, colony summer weight gain, and Varroa mite incidence were measured. I found that applications of over-the-counter probiotics did not improve the measured parameters of honeybee health compared to a control group. Results suggest that probiotic application was not beneficial but positive benefits may depend on application method, brand, or strain of probiotics used. I also examined how colony health metrics responded to different …


One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review, Evanson R. Omuse, Honest Machekano, Bonoukpoè M. Sokame, Daniel M. Mutyambai, Thomas Dubois, Sevgan Subramanian, Frank Chidawanyika Jun 2025

One Health Interventions And Challenges Under Rural African Smallholder Farmer Settings: A Scoping Review, Evanson R. Omuse, Honest Machekano, Bonoukpoè M. Sokame, Daniel M. Mutyambai, Thomas Dubois, Sevgan Subramanian, Frank Chidawanyika

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The global human population is rapidly increasing, escalating interactions of people, animals and the environment. This has led to more convoluted health challenges, for which African smallholder farmers bear the brunt. The One Health (OH) approach recognises the interconnectedness of these health challenges and thus follows a transdisciplinary approach involving diverse stakeholders to address them. Although there have recently been global concerted efforts and studies on OH, an information gap exists in Africa, particularly regarding smallholder farmers and their needs. Additionally, evaluation frameworks and outcome metrics for OH initiatives are still unclear. Thus, this study was conducted to critically map …


Do Behavioral Attitudes Of Livestock Farmers In Cameroon Influence The Adoption Of Insect-Based Feed? An Application Of The Theory Of Planned Behavior, Ruth T. Nembu, Boris D. Soh Wenda, Hervé K. Mube, Hervé W. Napi, Shaphan Y. Chia, Sali A. Ndindeng, Rodrigue B. Kamga, Dorothy E. Fon May 2025

Do Behavioral Attitudes Of Livestock Farmers In Cameroon Influence The Adoption Of Insect-Based Feed? An Application Of The Theory Of Planned Behavior, Ruth T. Nembu, Boris D. Soh Wenda, Hervé K. Mube, Hervé W. Napi, Shaphan Y. Chia, Sali A. Ndindeng, Rodrigue B. Kamga, Dorothy E. Fon

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This study investigates the effects of behavioral factors on the adoption of insect-based feed technology among livestock farmers in Cameroon. We use partial least squares structural equation modeling (PLS-SEM) to analyze primary data collected from 131 poultry and fish farmers in the Centre region of Cameroon. Our findings reveal that 18.32 % of the surveyed farmers are users of insect-based feed, whereas 81.68 % are non-users. We also show that livestock farmers’ attitudes directly influence the decision to use insect-based feed. Our findings suggest that perceived behavior control and subjective norms indirectly reinforce the decision to use insect-based feed-through attitudes. …


Stomoxys Flies (Diptera, Muscidae) Are Competent Vectors Of Trypanosoma Evansi, Trypanosoma Vivax, And Other Livestock Hemopathogens, Julia W. Muita, Joel L. Bargul, John Mark O. Makwatta, Ernest M. Ngatia, Simon K. Tawich, Daniel K. Masiga, Merid N. Getahun May 2025

Stomoxys Flies (Diptera, Muscidae) Are Competent Vectors Of Trypanosoma Evansi, Trypanosoma Vivax, And Other Livestock Hemopathogens, Julia W. Muita, Joel L. Bargul, John Mark O. Makwatta, Ernest M. Ngatia, Simon K. Tawich, Daniel K. Masiga, Merid N. Getahun

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Stomoxys flies are widely distributed and economically significant vectors of various livestock pathogens of veterinary importance. However, the role of Stomoxys spp. in pathogen transmission is poorly understood. Therefore, we studied the feeding patterns of these blood feeders collected from specific locations in Kenya to identify various vertebrate hosts they fed on, the livestock hemopathogens they carried, and to elucidate their role in pathogens transmission. Our findings show that field-collected Stomoxys flies carried several pathogens, including Trypanosoma spp., Anaplasma spp., and Theileria spp., which were also detected in the blood of sampled livestock, namely camels and cattle. The findings on …


Defatted Black Soldier Fly Larvae Meal As A Substitute Of Soybean Meal In Dairy Cow Diets, D. J.M. Braamhaar, W. F. Pellikaan, D. List, D. Korir, C. M. Tanga, S. J. Oosting Apr 2025

Defatted Black Soldier Fly Larvae Meal As A Substitute Of Soybean Meal In Dairy Cow Diets, D. J.M. Braamhaar, W. F. Pellikaan, D. List, D. Korir, C. M. Tanga, S. J. Oosting

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The replacement of soybean meal (SBM) with insect meal, e.g. black soldier fly larvae meal (BSFLM), as an alternative protein source in livestock feed is gaining traction globally. To date, no in vivo studies have been conducted to test the impact of BSFLM on the milk production of dairy cows. This study quantified the effects of replacing SBM with defatted black soldier fly larvae meal (DBSFLM) in diets of lactating dairy cows on feed intake, milk production and composition, apparent total-tract digestibility, feed- and nitrogen use efficiency. Twelve lactating Holstein-Friesian cows were used in a replicated 3 × 3 Latin …


Feeding Habits And Malaria Parasite Infection Of Anopheles Mosquitoes In Selected Agroecological Areas Of Northwestern Ethiopia, Aklilu K. Belay, Abebe Asale, Catherine L. Sole, Abdullahi A. Yusuf, Baldwyn Torto, Clifford M. Mutero, David P. Tchouassi Dec 2024

Feeding Habits And Malaria Parasite Infection Of Anopheles Mosquitoes In Selected Agroecological Areas Of Northwestern Ethiopia, Aklilu K. Belay, Abebe Asale, Catherine L. Sole, Abdullahi A. Yusuf, Baldwyn Torto, Clifford M. Mutero, David P. Tchouassi

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Background: Surveillance of the host–anopheline mosquitoes’ interaction is important for assessing malaria transmission risk and guiding vector control. We assume that changes in malaria vector species’ feeding habits, as well as the surrounding environment, have a substantial impact on varied malaria transmission. In this study, we determined the vertebrate host feeding patterns of anopheline mosquitoes to characterize entomologic risk factors for malaria in Jabi Tehnan, Northwestern Ethiopia. Methods: Blood-fed anophelines surveyed during malaria surveillance in Jabi Tehnan district of northwestern Ethiopia were utilized in this study. They were collected using Centers for Disease Control and Prevention (CDC) light traps deployed …


Effect Of Use Of Tsetse Repellant Collar Technology On The Farm Performance And Household Welfare Of Small-Scale Livestock Farmers In Kenya, Beatrice W. Muriithi, Kassie Menale, Gracious M. Diiro, Michael N. Okal, Daniel K. Masiga Jun 2023

Effect Of Use Of Tsetse Repellant Collar Technology On The Farm Performance And Household Welfare Of Small-Scale Livestock Farmers In Kenya, Beatrice W. Muriithi, Kassie Menale, Gracious M. Diiro, Michael N. Okal, Daniel K. Masiga

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Tsetse-transmitted Animal African Trypanosomosis (AAT) is a disease of economic importance to livestock development in Sub-Saharan Africa. The disease is widely managed using trypanocides that are expensive for smallholders and associated with health risks due to drug residues in animal products and drug resistance. Developed recently by the International Centre of Insect Physiology and Ecology (icipe) and partners, the novel Tsetse repellent collar technology (TRCT) provides an effective alternative control measure that targets the tsetse fly vector of AAT. This study assessed the impact of TRCT on household welfare and food security by using ex-post non-experimental data obtained from 632 …


An Update On The Distribution Of Glossina (Tsetse Flies) At The Wildlife-Human-Livestock Interface Of Akagera National Park, Rwanda, Richard S. Gashururu, Samuel M. Githigia, Methode N. Gasana, Richard Habimana, Ndichu Maingi, Giuliano Cecchi, Massimo Paone, Weining Zhao, Daniel K. Masiga, James Gashumba Dec 2021

An Update On The Distribution Of Glossina (Tsetse Flies) At The Wildlife-Human-Livestock Interface Of Akagera National Park, Rwanda, Richard S. Gashururu, Samuel M. Githigia, Methode N. Gasana, Richard Habimana, Ndichu Maingi, Giuliano Cecchi, Massimo Paone, Weining Zhao, Daniel K. Masiga, James Gashumba

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Background: Glossina (tsetse flies) biologically transmit trypanosomes that infect both humans and animals. Knowledge of their distribution patterns is a key element to better understand the transmission dynamics of trypanosomosis. Tsetse distribution in Rwanda has not been well enough documented, and little is known on their current distribution. This study determined the current spatial distribution, abundance, diversity, and seasonal variations of tsetse flies in and around the Akagera National Park. Methods: A longitudinal stratified sampling following the seasons was used. Biconical traps were deployed in 55 sites for 6 consecutive days of each study month from May 2018 to June …


Occurrence, Diversity And Distribution Of Trypanosoma Infections In Cattle Around The Akagera National Park, Rwanda, S. Richard Gashururu, Ndichu Maingi, Samuel M. Githigia, Methode N. Gasana, Peter O. Odhiambo, Dennis O. Getange, Richard Habimana, Giuliano Cecchi, Weining Zhao, James Gashumba, Joel L. Bargul, Daniel K. Masiga Dec 2021

Occurrence, Diversity And Distribution Of Trypanosoma Infections In Cattle Around The Akagera National Park, Rwanda, S. Richard Gashururu, Ndichu Maingi, Samuel M. Githigia, Methode N. Gasana, Peter O. Odhiambo, Dennis O. Getange, Richard Habimana, Giuliano Cecchi, Weining Zhao, James Gashumba, Joel L. Bargul, Daniel K. Masiga

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Background African Trypanosomiases threaten the life of both humans and animals. Trypanosomes are transmitted by tsetse and other biting flies. In Rwanda, the African Animal Trypanosomiasis (AAT) endemic area is mainly around the tsetse-infested Akagera National Park (NP). The study aimed to identify Trypanosoma species circulating in cattle, their genetic diversity and distribution around the Akagera NP. Methodology A cross-sectional study was carried out in four districts, where 1,037 cattle blood samples were collected. The presence of trypanosomes was determined by microscopy, immunolog-ical rapid test VerY Diag and PCR coupled with High-Resolution Melt (HRM) analysis. A parametric test (ANOVA) was …


Molecular Characterization Of Trypanosoma Vivax In Tsetse Flies Confirms The Presence Of The Virulent Tvv4 Genotype In Kenya: Potential Implications For The Control Of Trypanosomiasis In Shimba Hills, Faith I. Ebhodaghe, Armanda D.S. Bastos, Kevin O. Kidambasi, Shewit Kalayou, Daniel K. Masiga, Michael N. Okal Sep 2021

Molecular Characterization Of Trypanosoma Vivax In Tsetse Flies Confirms The Presence Of The Virulent Tvv4 Genotype In Kenya: Potential Implications For The Control Of Trypanosomiasis In Shimba Hills, Faith I. Ebhodaghe, Armanda D.S. Bastos, Kevin O. Kidambasi, Shewit Kalayou, Daniel K. Masiga, Michael N. Okal

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Trypanosoma vivax is a vector-borne protozoan parasite of livestock endemic to Africa and South America. To date, fifteen genotypes of the parasite have been described in vertebrate and insect hosts in East Africa. However, information regarding T. vivax diversity remains limited in many endemic countries in the sub-region, including Kenya. Such information could deepen insight into the local epidemiology of animal trypanosomiasis in Shimba Hills, a wildlife area in southeast Kenya where T. vivax is endemic and infects livestock. We employed two-gene conventional-PCR-sequencing and phylogenetic analysis to characterize T. vivax genotypes in tsetse flies collected between November 2018 and September …


Farmer Perceptions And Willingness To Pay For Novel Livestock Pest Control Technologies: A Case Of Tsetse Repellent Collar In Kwale County In Kenya, Beatrice W. Muriithi, Nancy G. Gathogo, Gracious M. Diiro, Michael M. Kidoido, Michael Nyangánga Okal, Daniel K. Masiga Aug 2021

Farmer Perceptions And Willingness To Pay For Novel Livestock Pest Control Technologies: A Case Of Tsetse Repellent Collar In Kwale County In Kenya, Beatrice W. Muriithi, Nancy G. Gathogo, Gracious M. Diiro, Michael M. Kidoido, Michael Nyangánga Okal, Daniel K. Masiga

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Tsetse-transmitted Animal African Trypanosomosis (AAT) is one of the most important constraints to livestock development in Africa. Use of trypanocides has been the most widespread approach for the management of AAT, despite the associated drug resistance and health con-cerns associated with drug metabolites in animal products. Alternative control measures that target tsetse fly vectors of AAT, though effective, have been hard to sustain in part because these are public goods applied area-wide. The International Centre of Insect Physiology and Ecology (icipe) and partners have developed and implemented a novel tsetse repellent collar (TRC) applied on animals to limit contact of …


A Survey Of Mosquito-Borne And Insect-Specific Viruses In Hospitals And Livestock Markets In Western Kenya, Tatenda Chiuya, Daniel K. Masiga, Laura C. Falzon, Armanda D.S. Bastos, Eric M. Fèvre, Jandouwe Villinger May 2021

A Survey Of Mosquito-Borne And Insect-Specific Viruses In Hospitals And Livestock Markets In Western Kenya, Tatenda Chiuya, Daniel K. Masiga, Laura C. Falzon, Armanda D.S. Bastos, Eric M. Fèvre, Jandouwe Villinger

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Aedes aegypti and Culex pipiens complex mosquitoes are prolific vectors of arboviruses that are a global threat to human and animal health. Increased globalization and ease of travel have facilitated the worldwide dissemination of these mosquitoes and the viruses they transmit. To assess disease risk, we determined the frequency of arboviruses in western Kenyan counties bordering an area of high arboviral activity. In addition to pathogenic viruses, insect-specific flaviviruses (ISFs), some of which are thought to impair the transmission of specific pathogenic arboviruses, were also evaluated. We trapped mosquitoes in the short and long rainy seasons in 2018 and 2019 …


Push-Pull Strategy Combined With Net Houses For Controlling Cowpea Insect Pests And Enhancing Crop Yields, Seydou Diabate, Thibaud Martin, Lucy Kananu Murungi, Komi K.M. Fiaboe, John Wesonga, John M. Kimani, Emilie Deletre Mar 2021

Push-Pull Strategy Combined With Net Houses For Controlling Cowpea Insect Pests And Enhancing Crop Yields, Seydou Diabate, Thibaud Martin, Lucy Kananu Murungi, Komi K.M. Fiaboe, John Wesonga, John M. Kimani, Emilie Deletre

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Net houses can be used in tropical environments to protect crops such as cowpea against large insect pests, thereby avoiding pesticide treatments while sustainably mitigating the effects of climate change. We investigated a push-pull strategy to prevent small insect pest outbreaks in a net house. The push component consisted of two stimulus plants, i.e. Cymbopogon citratus and Tagetes minuta, and the pull stimuli consisted of visual cues from blue and yellow sticky traps. Field experiments were set up in central Kenya and conducted during a rainy and a dry season, involving an open field control treatment, and three management treatments …


Tsetse Blood-Meal Sources, Endosymbionts And Trypanosome-Associations In The Maasai Mara National Reserve, A Wildlife-Human-Livestock Interface, Edward Edmond Makhulu, Jandouwe Villinger, Vincent Owino Adunga, Maamun M. Jeneby, Edwin Murungi Kimathi, Enock Mararo, Joseph Wang’Ang’A Oundo, Ali Abdulahi Musa, Lillian Wambua Jan 2021

Tsetse Blood-Meal Sources, Endosymbionts And Trypanosome-Associations In The Maasai Mara National Reserve, A Wildlife-Human-Livestock Interface, Edward Edmond Makhulu, Jandouwe Villinger, Vincent Owino Adunga, Maamun M. Jeneby, Edwin Murungi Kimathi, Enock Mararo, Joseph Wang’Ang’A Oundo, Ali Abdulahi Musa, Lillian Wambua

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African trypanosomiasis (AT) is a neglected disease of both humans and animals caused by Trypanosoma parasites, which are transmitted by obligate hematophagous tsetse flies (Glossina spp.). Knowledge on tsetse fly vertebrate hosts and the influence of tsetse endo-symbionts on trypanosome presence, especially in wildlife-human-livestock interfaces, is limited. We identified tsetse species, their blood-meal sources, and correlations between endosymbionts and trypanosome presence in tsetse flies from the trypanosome-endemic Maasai Mara National Reserve (MMNR) in Kenya. Among 1167 tsetse flies (1136 Glossina pallidipes, 31 Glossina swynnertoni) collected from 10 sampling sites, 28 (2.4%) were positive by PCR for trypanosome DNA, most (17/28) …


How To Turn Collectors Of Edible Insects Into Mini-Livestock Farmers: Multidimensional Sustainability Challenges To A Thriving Industry, Sarah Nischalke, Ingo Wagler, Chrysantus Tanga, David Allan, Chama Phankaew, Christian Ratompoarison, Andrianantenaina Razafindrakotomamonjy, Elizabeth Kusia Sep 2020

How To Turn Collectors Of Edible Insects Into Mini-Livestock Farmers: Multidimensional Sustainability Challenges To A Thriving Industry, Sarah Nischalke, Ingo Wagler, Chrysantus Tanga, David Allan, Chama Phankaew, Christian Ratompoarison, Andrianantenaina Razafindrakotomamonjy, Elizabeth Kusia

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Edible insects reared under suitable climatic conditions are an efficient and ecological food source. However, most of the insects come from wild collection and not from farms (FAO, 2013). To establish insects as part of a sustainable food environment, collectors, farmers or small entrepreneurs need to be transformed into mini-livestock farmers, but people who eat or collect insects do not necessarily want to rear them. This paper presents four case studies from Kenya, Madagascar, Myanmar and Thailand, which are in different stages of sector development to show pathways for establishing insect rearing, and the associated economic, resource/ecological and social challenges …


Integrated Management Of Aphis Craccivora In Cowpea Using Intercropping And Entomopathogenic Fungi Under Field Conditions, Allan Mweke, Komivi Senyo Akutse, Christian Ulrichs, Komi Kouma Mokpokpo Fiaboe, Nguya Kalemba Maniania, Sunday Ekesi Jun 2020

Integrated Management Of Aphis Craccivora In Cowpea Using Intercropping And Entomopathogenic Fungi Under Field Conditions, Allan Mweke, Komivi Senyo Akutse, Christian Ulrichs, Komi Kouma Mokpokpo Fiaboe, Nguya Kalemba Maniania, Sunday Ekesi

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Cowpea aphid, Aphis craccivora, is a major cowpea pest. Cowpea–cereal intercrop alone does not effectively manage the pest. Use of pesticides in intercrop leads to health and environmental risks. Fungal-based biopesticides offer a better option because they are environment-and consumer-friendly. This study assessed the combined effect of Metarhizium anisopliae ICIPE 62 and cowpea–maize intercrop against A. craccivora under six treatments: (1) untreated cowpea monocrop, (2) untreated cowpea–maize intercrop, (3) cowpea monocrop + ICIPE 62, (4) cowpea–maize intercrop + ICIPE 62, (5) cowpea monocrop + Duduthrin insecticide, and (6) cowpea–maize intercrop + Duduthrin during three seasons (long rainy/cold and dry/short rainy). …


A Historical Review Of Management Options Used Against The Stable Fly (Diptera: Muscidae), David F. Cook Apr 2020

A Historical Review Of Management Options Used Against The Stable Fly (Diptera: Muscidae), David F. Cook

Animal production and livestock research articles

The stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), remains a significant economic pest globally in situations where intensive animal production or horticultural production provide a suitable developmental medium. Stable flies have been recorded as pests of livestock and humans since the late 1800s to early 1900s. Over 100 years of research has seen numerous methodologies used to control this fly, in particular to protect cattle from flies to minimise production losses. Reduced milk production in dairy cows and decreased weight gain in beef cattle account for losses in the US alone of > $2000 million annually. Rural lifestyles and recreation are …


Burial And Compaction Of Sandy Soils To Prevent Emergence Of Adult Stable Fly (Diptera: Muscidae) At The Soil Surface, David F. Cook, Robert A. Deyl, Jeremy B. Lindsey, Mario F. D'Antuono, Don Telfer, Ian R. Mcpharlin Feb 2020

Burial And Compaction Of Sandy Soils To Prevent Emergence Of Adult Stable Fly (Diptera: Muscidae) At The Soil Surface, David F. Cook, Robert A. Deyl, Jeremy B. Lindsey, Mario F. D'Antuono, Don Telfer, Ian R. Mcpharlin

Horticulture Research Articles

Stable fly (Stomoxys calcitrans L.) remain a significant pest affecting livestock and rural communities on the Swan Coastal Plain around Perth, Western Australia. Vegetable crop residues remaining after harvest enable stable fly development. Left untreated they can produce from several hundred to >1,000 stable fly/m2 of post-harvest residues. We studied the effect of burial and compaction of sandy soils on adult emergence of stable fly and house fly (Musca domestica L.) (Diptera: Muscidae). Adults of both fly species can move up through 50 cm of loose, dry sand, however at depths greater than 60 cm, emergence rapidly declines …


Experimental Feeding Studies With Crickets And Locusts On The Use Of Feed Mixtures Composed Of Storable Feed Materials Commonly Used In Livestock Production, P. Straub, C. M. Tanga, I. Osuga, W. Windisch, S. Subramanian Aug 2019

Experimental Feeding Studies With Crickets And Locusts On The Use Of Feed Mixtures Composed Of Storable Feed Materials Commonly Used In Livestock Production, P. Straub, C. M. Tanga, I. Osuga, W. Windisch, S. Subramanian

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Insects such as the Mediterranean field cricket, Gryllus bimaculatus and the Desert locust, Schistocerca gregaria, are emerging as potential sources of human food and feed for livestock. High nutritive value and efficient feed conversion make them attractive for commercial production as novel livestock, but these properties strongly vary with the insects´ diet. Current mass-rearing protocols are based on fresh, non-storable feed materials. This requires constant supply and makes the systems sensitive to fluctuations regarding nutritional quality and safety. Hence there is a need to find storable, readily available feeds. Therefore, experimental diets were composed from the five different feed materials, …


Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender May 2018

Radical Social Ecology As Deep Pragmatism: A Call To The Abolition Of Systemic Dissonance And The Minimization Of Entropic Chaos, Arielle Brender

Student Theses 2015-Present

This paper aims to shed light on the dissonance caused by the superimposition of Dominant Human Systems on Natural Systems. I highlight the synthetic nature of Dominant Human Systems as egoic and linguistic phenomenon manufactured by a mere portion of the human population, which renders them inherently oppressive unto peoples and landscapes whose wisdom were barred from the design process. In pursuing a radical pragmatic approach to mending the simultaneous oppression and destruction of the human being and the earth, I highlight the necessity of minimizing entropic chaos caused by excess energy expenditure, an essential feature of systems that aim …


Haematology Of N’Dama And West African Shorthorn Cattle Herds Under Natural Trypanosoma Vivax Challenge In Ghana, Ebenezer Yaw Ganyo, Johnson N. Boampong, Daniel K. Masiga, Jandouwe Villinger, Paa Kobina Turkson Jan 2018

Haematology Of N’Dama And West African Shorthorn Cattle Herds Under Natural Trypanosoma Vivax Challenge In Ghana, Ebenezer Yaw Ganyo, Johnson N. Boampong, Daniel K. Masiga, Jandouwe Villinger, Paa Kobina Turkson

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Background: Animal trypanosomosis is a major cause of economic loss in livestock production in Africa. A suggested control measure is to use breeds with traits of trypanotolerance. The study examines the effect of natural Trypanosoma vivax challenge on haematological parameters in two trypanotolerant cattle [N’Dama and West African Shorthorn (WASH)] herds. Methods: Trypanosoma vivax-specific primers were used to diagnose T. vivax infection in an N’Dama herd at Cape Coast in southern Ghana and a WASH herd at Chegbani in northern Ghana from May to July 2011 in a cross-sectional study. Levels of haematological parameters comprising packed cell volume (PCV), haemoglobin …


Protecting Cows In Small Holder Farms In East Africa From Tsetse Flies By Mimicking The Odor Profile Of A Non-Host Bovid, Rajinder K. Saini, Benedict O. Orindi, Norber Mbahin, John A. Andoke, Peter N. Muasa, David M. Mbuvi, Caroline M. Muya, John A. Pickett, Christian W. Borgemeister Oct 2017

Protecting Cows In Small Holder Farms In East Africa From Tsetse Flies By Mimicking The Odor Profile Of A Non-Host Bovid, Rajinder K. Saini, Benedict O. Orindi, Norber Mbahin, John A. Andoke, Peter N. Muasa, David M. Mbuvi, Caroline M. Muya, John A. Pickett, Christian W. Borgemeister

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Background: For the first time, differential attraction of pathogen vectors to vertebrate animals is investigated for novel repellents which when applied to preferred host animals turn them into non-hosts thereby providing a new paradigm for innovative vector control. For effectively controlling tsetse flies (Glossina spp.), vectors of African trypanosomosis, causing nagana, repellents more powerful than plant derived, from a non-host animal the waterbuck, Kobus ellipsiprymnus defassa, have recently been identified. Here we investigate these repellents in the field to protect cattle from nagana by making cattle as unattractive as the buck. Methodology/Principal findings: To dispense the waterbuck repellents comprising guaiacol, …


Storage Of Pigeonpea Grain (Cajanus Cajan (L.) Millsp.) In Hermetic Triple-Layer Bags Prevents Losses Caused By Callosobruchus Maculatus (F.) (Coleoptera: Bruchidae), H. D. Affognon, A. W. Njoroge, C. M. Mutungi, J. Manono, D. Baributsa, L. L. Murdock Jul 2016

Storage Of Pigeonpea Grain (Cajanus Cajan (L.) Millsp.) In Hermetic Triple-Layer Bags Prevents Losses Caused By Callosobruchus Maculatus (F.) (Coleoptera: Bruchidae), H. D. Affognon, A. W. Njoroge, C. M. Mutungi, J. Manono, D. Baributsa, L. L. Murdock

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Dry pigeonpea grain is susceptible to infestation from the cowpea bruchid during storage. This study investigated the potential of Purdue Improved Cowpea Storage (PICS™) and polypropylene (PP) bags for reducing insect infestation under ambient laboratory conditions for six months. Some of the bags were artificially infested with cowpea bruchid Callosobruchus maculatus (PICS1, PP1) while others were not (PICS0, PP0). In an additional trial, PP bags containing pigeonpeas were treated with Actellic Super® dust before being artificially infested (PP1Ac). Moisture content, number of live C. maculatus adults, grain damage, and weight loss were determined at monthly intervals. Temperature and relative humidity …


Active Aggregation Among Sexes In Bean Flower Thrips (Megalurothrips Sjostedti) On Cowpea (Vigna Unguiculata), Saliou Niassy, Sunday Ekesi, Nguya K. Maniania, Benedict Orindi, Gerald B. Moritz, Willem J. De Kogel, Sevgan Subramanian Jan 2016

Active Aggregation Among Sexes In Bean Flower Thrips (Megalurothrips Sjostedti) On Cowpea (Vigna Unguiculata), Saliou Niassy, Sunday Ekesi, Nguya K. Maniania, Benedict Orindi, Gerald B. Moritz, Willem J. De Kogel, Sevgan Subramanian

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Male sexual aggregations are a common territorial, mating-related or resource-based, behaviour observed in diverse organisms, including insects such as thrips. The influence of factors such as plant substrate, time of day, and geographic location on aggregation of thrips is uncertain, therefore we monitored the dispersion of male and female bean flower thrips (BFT), Megalurothrips sjostedti (Trybom) (Thysanoptera: Thripidae), on cowpea, Vigna unguiculata (L.) Walp. (Fabaceae), over three cowpea growth stages and across three cowpea-growing areas of Kenya. Our results indicated that for all the crop growth stages, the density of BFTs varied over the time of day, with higher densities …


Mosquito Host Choices On Livestock Amplifiers Of Rift Valley Fever Virus In Kenya, David P. Tchouassi, Robinson O.K. Okiro, Rosemary Sang, Lee W. Cohnstaedt, David Scott Mcvey, Baldwyn Torto Jan 2016

Mosquito Host Choices On Livestock Amplifiers Of Rift Valley Fever Virus In Kenya, David P. Tchouassi, Robinson O.K. Okiro, Rosemary Sang, Lee W. Cohnstaedt, David Scott Mcvey, Baldwyn Torto

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Background: Animal hosts may vary in their attraction and acceptability as components of the host location process for assessing preference, and biting rates of vectors and risk of exposure to pathogens. However, these parameters remain poorly understood for mosquito vectors of the Rift Valley fever (RVF), an arboviral disease, and for a community of mosquitoes. Methods: Using three known livestock amplifiers of RVF virus including sheep, goat and cattle as bait in enclosure traps, we investigated the host-feeding patterns for a community of mosquitoes in Naivasha, an endemic area of Rift Valley fever (RVF), in a longitudinal study for six …


Unraveling Host-Vector-Arbovirus Interactions By Two-Gene High Resolution Melting Mosquito Bloodmeal Analysis In A Kenyan Wildlife-Livestock Interface, David Omondi, Daniel K. Masiga, Yvonne Ukamaka Ajamma, Burtram C. Fielding, Laban Njoroge, Jandouwe Villinger Jul 2015

Unraveling Host-Vector-Arbovirus Interactions By Two-Gene High Resolution Melting Mosquito Bloodmeal Analysis In A Kenyan Wildlife-Livestock Interface, David Omondi, Daniel K. Masiga, Yvonne Ukamaka Ajamma, Burtram C. Fielding, Laban Njoroge, Jandouwe Villinger

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The blood-feeding patterns of mosquitoes are directly linked to the spread of pathogens that they transmit. Efficient identification of arthropod vector bloodmeal hosts can identify the diversity of vertebrate species potentially involved in disease transmission cycles. While molecular bloodmeal analyses rely on sequencing of cytochrome b (cyt b) or cytochrome oxidase 1 gene PCR products, recently developed bloodmeal host identification based on high resolution melting (HRM) analyses of cyt b PCR products is more cost-effective. To resolve the diverse vertebrate hosts that mosquitoes may potentially feed on in sub-Saharan Africa, we utilized HRM profiles of both cyt b and 16S …


Distribution And Diversity Of The Vectors Of Rift Valley Fever Along The Livestock Movement Routes In The Northeastern And Coastal Regions Of Kenya, Samwel O. Arum, Christopher W. Weldon, Benedict Orindi, Tobias Landmann, David P. Tchouassi, Hippolyte D. Affognon, Rosemary Sang May 2015

Distribution And Diversity Of The Vectors Of Rift Valley Fever Along The Livestock Movement Routes In The Northeastern And Coastal Regions Of Kenya, Samwel O. Arum, Christopher W. Weldon, Benedict Orindi, Tobias Landmann, David P. Tchouassi, Hippolyte D. Affognon, Rosemary Sang

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Background: Knowledge of vector ecology is important in understanding the transmission dynamics of vector borne disease. In this study, we determined the distribution and diversity of mosquitoes along the major nomadic livestock movement routes (LMR) in the traditional pastoral ecozone of northeastern Kenya. We focused on the vectors of Rift Valley fever virus (RVFv) with the aim of understanding their ecology and how they can potentially influence the circulation of RVFv. Methods: Mosquito surveys were conducted during the short and long rainy seasons from November 2012 to August 2014 using CO2-baited CDC light traps at seven sites selected for their …


Pathogenicity And Performance Of Two Candidate Isolates Of Metarhizium Anisopliae And Beauveria Bassiana (Hypocreales: Clavicipitaceae) In Four Liquid Culture Media For The Management Of The Legume Pod Borer Maruca Vitrata (Lepidoptera: Crambidae), V. Tumuhaise, S. Ekesi, S. A. Mohamed, P. N. Ndegwa, L. W. Irungu, R. Srinivasan, N. K. Maniania Mar 2015

Pathogenicity And Performance Of Two Candidate Isolates Of Metarhizium Anisopliae And Beauveria Bassiana (Hypocreales: Clavicipitaceae) In Four Liquid Culture Media For The Management Of The Legume Pod Borer Maruca Vitrata (Lepidoptera: Crambidae), V. Tumuhaise, S. Ekesi, S. A. Mohamed, P. N. Ndegwa, L. W. Irungu, R. Srinivasan, N. K. Maniania

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The aim of this study was to identify and develop entomopathogenic fungi as biopesticides for management of Maruca vitrata (Fabricius). Fourteen isolates of Metarhizium anisopliae (Metchnikoff) Sorokin and six of Beauveria bassiana (Bals.-Criv.) Vuill. were screened against first instar larvae of M. vitrata. The two most virulent isolates were selected and further tested for production in four liquid media. Different formulations of one isolate were evaluated against different developmental stages. Metarhizium anisopliae ICIPE 18 and ICIPE 69 caused highest larval mortality of 91 and 81%, with lethal time to 50% mortality (LT50) values of 1.8 and 1.7 days and LC50 …


Whole Genome Phylogenetic Investigation Of A West Nile Virus Strain Isolated From A Tick Sampled From Livestock In North Eastern Kenya, Olivia Wesula Lwande, Marietjie Venter, Joel Lutomiah, George Michuki, Cecilia Rumberia, Francis Gakuya, Vincent Obanda, Caroline Tigoi, Collins Odhiambo, Fredrick Nindo, Samwel Symekher, Rosemary Sang Jan 2014

Whole Genome Phylogenetic Investigation Of A West Nile Virus Strain Isolated From A Tick Sampled From Livestock In North Eastern Kenya, Olivia Wesula Lwande, Marietjie Venter, Joel Lutomiah, George Michuki, Cecilia Rumberia, Francis Gakuya, Vincent Obanda, Caroline Tigoi, Collins Odhiambo, Fredrick Nindo, Samwel Symekher, Rosemary Sang

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Background: West Nile virus (WNV) has a wide geographical distribution and has been associated to cause neurological disease in humans and horses. Mosquitoes are the traditional vectors for WNV; however, the virus has also been isolated from tick species in North Africa and Europe which could be a means of introduction and spread of the virus over long distances through migratory birds. Although WNV has been isolated in mosquitoes in Kenya, paucity of genetic and pathogenicity data exists. We previously reported the isolation of WNV from ticks collected from livestock and wildlife in Ijara District of Kenya, a hotspot for …


Parasitological Prevalence Of Bovine Trypanosomosis In Kubo Division Of Kwale County Of Coastal: Baseline Survey, N. Mbahin, H. Affognon, J. Andoke, M. Tiberius, D. Mbuvi, J. Otieno, P. Muasa, R. K. Saini Apr 2013

Parasitological Prevalence Of Bovine Trypanosomosis In Kubo Division Of Kwale County Of Coastal: Baseline Survey, N. Mbahin, H. Affognon, J. Andoke, M. Tiberius, D. Mbuvi, J. Otieno, P. Muasa, R. K. Saini

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Prior to field trials of large scale tsetse repellent technology validation in July 2011, a cross-sectional survey of bovine trypanosomosis and its vectors was carried out to know the disease level in Kwale County of Coastal Kenya. A total of 584 adult cattle were examined at ten different Locations. The trypanosome prevalence in cattle with regard to age, sex, location and apparent tsetse density was investigated. The overall trypanosome prevalence in cattle in Kubo Division of Kwale County was 33.9%. Two types of infections were recorded in various parts of the Division in the following proportions: T. congolense 61.1%, T. …