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Articles 361 - 390 of 4315
Full-Text Articles in Aquaculture and Fisheries
Diet, Growth, And Survival Of Juvenile Coho Salmon (Oncorhynchus Kisutch) In Off-Channel Restoration Projects In Humboldt Bay Tributaries, Katherine Rose Stonecypher
Diet, Growth, And Survival Of Juvenile Coho Salmon (Oncorhynchus Kisutch) In Off-Channel Restoration Projects In Humboldt Bay Tributaries, Katherine Rose Stonecypher
Cal Poly Humboldt theses and projects
Anthropogenic degradation of off-channel floodplain habitats has contributed to widespread declines in Pacific salmonid populations. These habitats are critical to the survival and growth of juvenile Coho Salmon during their first year of life. Restoring connectivity between mainstem and floodplain habitats could provide juvenile salmonids access to a mosaic of heterogenous foraging opportunities, with important consequences for salmonid growth and survival. I analyzed the diet, growth, and survival of juvenile Coho Salmon rearing in three restored off-channel ponds and adjacent mainstem habitats in Humboldt Bay tributaries to evaluate project performance. Juvenile Coho Salmon diet composition was significantly different among sites …
Understanding Angler Responses To Fish Stocking Programs Across South Dakota, Riley L. Mounsdon
Understanding Angler Responses To Fish Stocking Programs Across South Dakota, Riley L. Mounsdon
Electronic Theses and Dissertations
Fisheries management is a complex process that often requires consideration of many factors to be implemented effectively. Stocking is one of the most often used fisheries management techniques for addressing a myriad of issues in the fisheries field. Stocking is also one of the largest expenses for many fisheries management agencies. Due to license sale declines, management actions including stocking, are being evaluated by many agencies to determine if there are ways to improve efficiency and effectiveness to reduce costs and enhance angler participation. For my second chapter, I evaluated the current fish stocking decision-making process within South Dakota Game, …
Effects Of Energy Drink Additives On Crayfish Metabolism, Katherine Paltz
Effects Of Energy Drink Additives On Crayfish Metabolism, Katherine Paltz
Williams Honors College, Honors Research Projects
The effects of caffeine and additives commonly found in energy drinks were tested on crayfish to record changes, if any, in their metabolism. Three experimental groups were studied: 1. Caffeine; 2. Caffeine + Glucuronolactone; 3.) Caffeine + Glucuronolactone + Taurine. Caffeine is a common stimulant used to improve mental awareness, headaches, memory, and athletic performance. Glucuronolactone is connected with cardiovascular issues and aggressive behavior. Taurine is said to improve mood and focus. The experiment was performed at two different caffeine levels: 600mg and 1200mg for an initial 1 hour of exposure and 10 hours after exposure used as a pseudo-baseline. …
Crayfish Tube-Feeding Methodology, Phoenix Johnson
Crayfish Tube-Feeding Methodology, Phoenix Johnson
Williams Honors College, Honors Research Projects
The purpose of this paper was to use a tube feeding method on crayfish to expose them to substances through ingestion rather than the gill. This was done to mimic human ingestion of substance to aid in crayfish research involving substances that are normally ingestion. This was done by using a pipette to inject grains of sand into the mouth of the crayfish. To determine if the method worked and successfully went into the stomach the grains of sand were recovered and counted under a microscope after certain increments of time post injection.
Investigating The Relationships Between Biotic And Abiotic Factors Affecting Migratory Behavior In Westslope Cutthroat Trout Within The Priest River System, Kyle M. Keenan
EWU Masters Thesis Collection
Numerous salmonid species demonstrate diverse life histories, encompassing both resident and migratory behaviors. Migratory life histories play a pivotal role in maintaining metapopulations for various reasons, facilitating genetic exchange between populations and yielding larger-sized females with increased fecundity relative to residents. This study took place in northern Idaho, within the lower Priest River system, which is highly susceptible to degrading habitat. Previous research has discovered this system as a significant contributor to migratory Westslope Cutthroat Trout (Oncorhynchus lewisi, WCT) in the Lake Pend Oreille and Priest River systems. Expanding on these results, we compared four Priest River tributaries that produced …
The Effects Of Warmer Temperatures On The Physiology Of Invasive Sea Lamprey And Imperiled Populations Of Lake Sturgeon In The Laurentian Great Lakes, Milica Koledin
Theses and Dissertations (Comprehensive)
Invasive sea lamprey (Petromyzon marinus) contributed to massive declines in fisheries in the Laurentian Great Lakes due to their parasitism of large-bodied salmonid fishes in the 20th century. Chemical control measures, which involve the application of 3-trifluoromethyl-4-nitrophenol (TFM) into rivers containing larval sea lamprey, were successfully implemented approximately 60 years ago to control populations. Recently, however, it was discovered that TFM tolerance increases in sea lamprey with a rise in temperature for reasons which are incompletely understood. Nor is it understood how sea lamprey will respond to warmer temperatures due to climate change, which could affect their …
Complex Dynamics Of Coral Gene Expression Responses To Low Ph Across Species, Veronica Z. Radice, Ana Martinez, Adina Paytan, Donald C. Potts, Daniel J. Barshis
Complex Dynamics Of Coral Gene Expression Responses To Low Ph Across Species, Veronica Z. Radice, Ana Martinez, Adina Paytan, Donald C. Potts, Daniel J. Barshis
Biological Sciences Faculty Publications
Coral capacity to tolerate low pH affects coral community composition and, ultimately, reef ecosystem function. Low pH submarine discharges (‘Ojo’; Yucatán, México) represent a natural laboratory to study plasticity and acclimatization to low pH in relation to ocean acidification. A previous >2‐year coral transplant experiment to ambient and low pH common garden sites revealed differential survivorship across species and sites, providing a framework to compare mechanistic responses to differential pH exposures. Here, we examined gene expression responses of transplants of three species of reef‐building corals (Porites astreoides, Porites porites and Siderastrea siderea) and their algal endosymbiont communities …
Baby's First Jets: A Kinematic And Hydrodynamic Analysis Of Turning In Cuttlefish Hatchlings, Alissa M. Ganley, Paul S. Krueger, Ian K. Bartol
Baby's First Jets: A Kinematic And Hydrodynamic Analysis Of Turning In Cuttlefish Hatchlings, Alissa M. Ganley, Paul S. Krueger, Ian K. Bartol
Biological Sciences Faculty Publications
Turning is an important aspect of life underwater, playing integral roles in predator avoidance, prey capture, and communication. While turning abilities have been explored in a diversity of adult nekton, little is currently known about turning in early ontogeny, especially for cephalopods. In this study, we investigated the turning abilities of hatchling common cuttlefish (Sepia officinalis, n = 49) and dwarf cuttlefish (Sepia bandensis, n = 30), using both kinematic and wake-based analyses. Using body tracking software and particle image velocimetry (PIV), we found that S. officinalis turned faster than S. bandensis, but both species …
Abalones At Risk: A Global Red List Assessment Of Haliotis In A Changing Climate, Howard Peters, Gina M. Ralph, Laura Rogers-Bennett
Abalones At Risk: A Global Red List Assessment Of Haliotis In A Changing Climate, Howard Peters, Gina M. Ralph, Laura Rogers-Bennett
Biological Sciences Faculty Publications
There is increasing awareness that marine invertebrates such as abalones are at risk from the combined stressors of fishing and climate change. Abalones are an important marine fishery resource and of cultural importance to Indigenous and non-Indigenous people. A highly priced marine delicacy, they are inherently vulnerable: individuals are slow-growing and long-lived and successful reproduction requires dense assemblages. However, their global conservation status is poorly understood. Using IUCN Red List methodology, we assessed the extinction risk to all 54 species of abalone (genus Haliotis). Of the 21 fished commercially for human consumption either now and/or in the past, 15 …
Population Demographics Of Channel Catfish In The Lower Wabash River, Valerie J. Thompson
Population Demographics Of Channel Catfish In The Lower Wabash River, Valerie J. Thompson
Masters Theses
Inland fisheries are experiencing increasing demands due to anthropogenic stressors, especially within large river systems that support both recreational and commercial fisheries. Riverine species are sensitive to habitat degradation, species introductions, and exploitation, so continued monitoring and management of these communities are vital to maintain abundance and diversity within these systems. The North American catfish family, Ictaluridae, supports both recreational and commercial fisheries throughout the United States. Channel Catfish Ictalurus punctatus are heavily studied and managed compared to other sport catfishes in this family because of their economic and ecological significance. One important Channel Catfish fishery exists in the lower …
Acute And Chronic Impacts Of A Biofilm, Activated Sludge, And Ultraviolet Radiation Treatment On The Fathead Minnow, Pimephales Promelas, Julia Fitch
Masters Theses
Wastewater treatment plants (WWTPs) are a point source of pollution that have potentially damaging effects on downstream fish populations, including reduced growth and survival and reproductive complications. To examine these possible effects, fathead minnows were exposed to effluent of three wastewater treatment processes at the Charleston Wastewater Treatment Plant located in Charleston, Illinois. These three treatments included two secondary treatments, a biofilm treatment and an activated sludge treatment, and one tertiary U.V. treatment. Two trials took place during this project: an acute, two-week exposure trial, and a chronic 119 day exposure trial. Upon completion of each trial, fish were assessed …
Improving Stream Connectivity In South Dakota, Colton Rainier Curtis
Improving Stream Connectivity In South Dakota, Colton Rainier Curtis
Electronic Theses and Dissertations
Stream connectivity is crucial for fish movement and genetic diversity in fragmented landscapes. Road-crossings often act as barriers, disrupting hydrology and isolating fish populations. The Southeast Aquatic Resources Partnership (SARP) has developed rapid road-crossing assessment protocols and tools to prioritize restoration projects. However, managers lack tools for planning, directing, and initiating roadcrossing assessments. Managers also require modeling approaches to effectively identify and monitor road-crossings across dynamic stream networks. Presented here is a case study offering a roadmap for effective implementation of collaborative road-crossing assessments and prioritization of remediation projects, alongside an exploration of predictive modeling approaches to identify problematic crossings …
Studying Redtail And Silver Surfperch In Sandy Beach Surf Zones With Mpas In Humboldt County, California, Usa, Noah G. Angell
Studying Redtail And Silver Surfperch In Sandy Beach Surf Zones With Mpas In Humboldt County, California, Usa, Noah G. Angell
Cal Poly Humboldt theses and projects
The sandy beach surf zone (SBSZ) represents an ecologically, economically, and culturally important marine habitat around the world. Despite its significance, the SBSZ has been historically underrepresented in marine and coastal ecology research. This in turn has caused many inshore marine species that prefer this habitat to be largely ignored. Two particular species, the Redtail surfperch Amphistichus rhodoterus and the Silver surfperch Hyperprosopon ellipticum, have not been well studied in the PNW region . There are merely three studies conducted on the Redtail surfperch in the Pacific Northwest region and the Silver surfperch has received even less research attention with …
Evaluating The Utility Of Tracers To Characterize Environmental Dna Transport And Inform Detection Of Fishes In Small Streams, Gavin B. Bandy
Evaluating The Utility Of Tracers To Characterize Environmental Dna Transport And Inform Detection Of Fishes In Small Streams, Gavin B. Bandy
Cal Poly Humboldt theses and projects
This study was motivated by the need to develop a noninvasive and highly sensitive monitoring tool for determining local occupancy of an endangered aquatic species To make inference into the occupancy of fishes within small stream sites, we developed a method to determine whether environmental DNA (eDNA) concentrations from a target species were elevated relative to a tracer, formulated to mimic eDNA and introduced at a single location. We examined patterns in the observed distribution of the tracer to account for the effects of site-specific transport processes and sampling on distributions of naturally occurring eDNA at small spatial scales (400 …
Methods For Optimizing Detection And Evaluating Habitat Use Of North American River Otters In South Dakota, Jessica Speiser
Methods For Optimizing Detection And Evaluating Habitat Use Of North American River Otters In South Dakota, Jessica Speiser
Electronic Theses and Dissertations
North American river otters (Lontra canadensis) play a critical role as an apex predator in riverine ecosystems. A challenging species to survey using traditional methods, they remain understudied in non-forested regions, such as the Northern Great Plains, where habitat degradation and landscape fragmentation may reduce habitat quality and availability and disrupt landscape connectivity. This thesis aims to improve detectability and investigates the habitat use of river otters in South Dakota to better understand habitat needs of otters and improve their continued recovery and management. My first chapter provides a brief overview of river otters and their ecological roles in freshwater …
The Impacts Of Anthropogenic Activity And Climate Change On The Formation Of Harmful Algal Blooms (Habs) And Its Ecological Consequence, Zhangxi Hu, Aifeng Li, Zhun Li, Margaret R. Mulholland
The Impacts Of Anthropogenic Activity And Climate Change On The Formation Of Harmful Algal Blooms (Habs) And Its Ecological Consequence, Zhangxi Hu, Aifeng Li, Zhun Li, Margaret R. Mulholland
OES Faculty Publications
No abstract provided.
Diel Vertical Migration Rates Of The Dinoflagellate Species Margalefidinium Polykrikoides In A Lower Chesapeake Bay Tributary, S. Clayton, Jacqueline B. Chrabot, Michael Echevarria, Leah Gibala-Smith, Kathryn Mogatas, Peter Bernhardt, Margaret R. Mulholland
Diel Vertical Migration Rates Of The Dinoflagellate Species Margalefidinium Polykrikoides In A Lower Chesapeake Bay Tributary, S. Clayton, Jacqueline B. Chrabot, Michael Echevarria, Leah Gibala-Smith, Kathryn Mogatas, Peter Bernhardt, Margaret R. Mulholland
OES Faculty Publications
Margalefidinium polykrikoides is a mixotrophic dinoflagellate harmful algal bloom (HAB) species that blooms annually in the lower Chesapeake Bay. M. polykrikoides undertakes a diel vertical migration (DVM) which may give it a competitive advantage over purely phototrophic organisms who cannot access deeper nutrient pools and allow it to form large toxic blooms. Laboratory-based estimates of M. polykrikoides’ DVM rates suggest that it is one of the fastest known dinoflagellate swimmers and understanding this behavior is likely important for modeling and predicting M. polykrikoides blooms. However, to date, no field-derived estimates of M. polykrikoides’ DVM rates have been made in the …
Cultivation Of A Commercially Important Macroalga (Ulva Sp) For Polyculture Research In Coastal Georgia, Kyle Mundy
Cultivation Of A Commercially Important Macroalga (Ulva Sp) For Polyculture Research In Coastal Georgia, Kyle Mundy
College of Graduate Studies: Theses & Dissertations
The Georgia coast has shown great potential for large-scale oyster aquaculture, and the rate at which the sector has grown demands implementation of novel techniques to ensure sustainability and success in the face of climate change. One practice – integrated multi-trophic aquaculture (IMTA) or polyculture – is known to have positive effects on the health and quality of farmed organisms and the surrounding environment through low pH/alkalinity amelioration, while also being a unique and viable way for a farming operation to diversify its products. To date, there has been no such attempt at investigating or demonstrating macroalgae culture in Georgia. …
Walleye (Sander Vitreus) Do Not Exhibit An Aggregative Predatory Response To Larval White Sturgeon (Acipenser Transmontanus) In The Upper Columbia River In Lake Roosevelt, Washington, Seth A. Barr
EWU Masters Thesis Collection
Seasonal prey abundance can drive predatory fishes to aggregate in areas of high prey density. This project aimed to determine whether Walleye (Sander vitreus) exhibit an aggregative predation response to larval White Sturgeon (Acipenser transmontanus) drifts in the Lake Roosevelt system which is a reservoir of the Columbia River in Washington State. Populations of sturgeon are threatened worldwide, and are in need of conservation research. Walleye are a popular nonnative piscivorous game fish that were accidentally or illegally introduced to Lake Roosevelt in the 1950’s. Walleye in this study were obtained by gillnetting during White Sturgeon pre-spawn, peak spawn, and …
Can Edna Be Used To Locate Shawnee Hills Cavefish Forbesichthys Papilliferus?, Megan Brandt, Timothy Spier Phd, Gary Zeruth Phd
Can Edna Be Used To Locate Shawnee Hills Cavefish Forbesichthys Papilliferus?, Megan Brandt, Timothy Spier Phd, Gary Zeruth Phd
Murray State Theses and Dissertations
Traditional stream fish sampling methods, such as seining and electrofishing, are time consuming, expensive, and limited to short stretches of stream. However, the analysis of environmental DNA (eDNA) from water samples promises to be a quick, inexpensive method for detecting fish. The streams of the Land Between the Lakes Recreational Area (LBL) are unique, undisturbed streams which contain many interesting fish species for which little information exists. One such rare species is the Shawnee Hills Cavefish Forbesichthys papilliferus, which is known to exist in a few LBL streams. However, the complete distribution of the Shawnee Hills Cavefish in LBL …
Economic Valuation Model Of Vanname Shrimp Culture And Mangrove Rehabilitation, Elrin Meivian Mongi Mongi, Mahawan Karuniasa, Mufti Petala Patria
Economic Valuation Model Of Vanname Shrimp Culture And Mangrove Rehabilitation, Elrin Meivian Mongi Mongi, Mahawan Karuniasa, Mufti Petala Patria
Journal of Environmental Science and Sustainable Development
The current climate change is caused by anthropogenic activities involving energy and industry usage that affect the temperature and climate of the Earth. The Paris Agreement on climate change is a commitment by some countries to act to address climate change by developing sustainably and emitting low levels of carbon dioxide (CO2). Changes in the oceans include temperature increases, acidification, and changes in currents, leading to decreased productivity in fishing and aquaculture. Integrated mangrove shrimp farming, known as silvofisheries, has been recognized as environmentally friendly cultivation. The implementation of silvofishery is a form of mangrove ecosystem management that integrates ecology …
Community Structure Of Sea Urchin As An Indicator Of The Ecological Balance Of Coastal Areas, Abdul Wahab Radjab, Intan Rabiyanti, Fiqih Muharby
Community Structure Of Sea Urchin As An Indicator Of The Ecological Balance Of Coastal Areas, Abdul Wahab Radjab, Intan Rabiyanti, Fiqih Muharby
Journal of Environmental Science and Sustainable Development
Sea urchins, soft-shelled invertebrates (Echinoderms), crucially contribute to cleaning the ocean bottom. However, sea urchins in tropical waters like Indonesia have not yet been fully disclosed. This study was conducted at Hutumuri and Rutong waters, Ambon City. This study assessed physical-chemical parameters, sea urchin species composition, classification, and community structure. Employing the Line Transect method with 5x5 m quadrants and 50m transect spacing, data analysis included density, relative density, frequency of presence, diversity, uniformity, and dominance indices. Results indicated normal temperature and salinity at both stations, supporting sea urchin life, with rocky and sandy substrates deemed suitable. A total of …
Feeding Overlap Investigations Between Hypophthalmichthys Molitrix And Cyprinus Carpio From Punjab, Pakistan, Afzala Munawar, Muhammad Imran, Muhammad Ameen, Abdul Majid Khan, Ayesha Sania, Muhammad Shahbaz
Feeding Overlap Investigations Between Hypophthalmichthys Molitrix And Cyprinus Carpio From Punjab, Pakistan, Afzala Munawar, Muhammad Imran, Muhammad Ameen, Abdul Majid Khan, Ayesha Sania, Muhammad Shahbaz
Journal of Bioresource Management
Freshwater ichthyofauna is facing intensive stress as a result of urbanization and growing industries in Pakistan. The alien fish species are considered one of the major challenges due to their deleterious effects. Feeding habit analysis is a basic tool to evaluate the ecological stress on fish species residing in different habitats. The present study was designed to disclose the feeding overlap between Cyprinus carpio (Common Carp) and Hypophthalmichthys molitrix (Silver Carp) from Head Marala (river Chenab) and Mangla Dam (river Jhelum). For this purpose, 20 samples i.e. 10 from each site of each fish species out of total 40, were …
A Large-Scale Experiment Finds No Consistent Evidence Of Change In Mortality Or Commercial Productivity In Silverlip Pearl Oysters (Pinctada Maxima) Exposed To A Seismic Source Survey, Miles J G Parsons, Diego R. Barneche, Conrad W. Speed, Robert D. Mccauley, Ryan D. Day, Cecile Dang, Rebecca Fisher, Hosna Gholipour-Kanani, Stephen J. Newman
A Large-Scale Experiment Finds No Consistent Evidence Of Change In Mortality Or Commercial Productivity In Silverlip Pearl Oysters (Pinctada Maxima) Exposed To A Seismic Source Survey, Miles J G Parsons, Diego R. Barneche, Conrad W. Speed, Robert D. Mccauley, Ryan D. Day, Cecile Dang, Rebecca Fisher, Hosna Gholipour-Kanani, Stephen J. Newman
Fisheries Research Articles
High-intensity, impulsive sounds are used to locate oil and gas reserves during seismic exploration of the seafloor. The impacts of this noise pollution on the health and mortality of marine invertebrates are not well known, including the silverlip pearl oyster (Pinctada maxima), which comprises one of the world's last remaining significant wildstock pearl oyster fisheries, in northwestern Australia. We exposed ≈11,000 P. maxima to a four-day experimental seismic survey, plus one vessel-control day. After exposure, survival rates were monitored throughout a full two-year production cycle, and the number and quality of pearls produced at harvest were assessed. Oysters from two …
Living Among Wildlife: Elevating Human-Wildlife Interactions And Coexistence, Bridget Rebecca Murphy
Living Among Wildlife: Elevating Human-Wildlife Interactions And Coexistence, Bridget Rebecca Murphy
Graduate Student Portfolios, Professional Papers, and Capstone Projects
After a semester of learning, both in class and in nature, my writing honed in further on this human-nature divide. To me, I see humans as part of nature – as we are mammals, animals, part of the food chain, biological beings no higher than others on our planet. We have simply constructed this false narrative around us within our societies, minds and media that embeds this division between us and nature, between us and wildlife. Humans have been managing, stewarding, living off and within landscapes for thousands of years. As time and technology evolved, a lot of people began …
A Year Of Wins For Farmed Animals, Lewis Bollard
A Year Of Wins For Farmed Animals, Lewis Bollard
General – Farm Animal Issues
Although the European Union shelved its ambitious farmed animal welfare reforms, there were other “wins” for farmed animals in 2023. There were 130 new corporate pledges to eliminate cages for laying hens and the worst abuses of broiler chickens. So far, over 1,000 corporate pledges have been fully implemented, and 39% of American hens, 60% of European hens, and 80% of British hens are cage-free, up from 6%, 41%, and 48%, respectively, a decade ago. The US Supreme Court upheld California’s Proposition 12, which bans the sale of eggs, pork, and veal from caged animals and their offspring in California. …
A Year Of Wins For Farmed Animals, Lewis Bollard
A Year Of Wins For Farmed Animals, Lewis Bollard
Nexus – UNEP – Animal Welfare, Environment, Sustainable Development
Although the European Union shelved its ambitious farmed animal welfare reforms, there were other “wins” for farmed animals in 2023. There were 130 new corporate pledges to eliminate cages for laying hens and the worst abuses of broiler chickens. So far, over 1,000 corporate pledges have been fully implemented, and 39% of American hens, 60% of European hens, and 80% of British hens are cage-free, up from 6%, 41%, and 48%, respectively, a decade ago. The US Supreme Court upheld California’s Proposition 12, which bans the sale of eggs, pork, and veal from caged animals and their offspring in California. …
Status Survey Of Eight Rare Aquatic Macroinvertebrates In East Texas, Alexandra Randall
Status Survey Of Eight Rare Aquatic Macroinvertebrates In East Texas, Alexandra Randall
Biology Theses
Freshwater is essential to human existence. The health of each freshwater source is connected to the survival of pollution intolerant species of aquatic macroinvertebrates in the orders Ephemeroptera, Plecoptera, and Trichoptera (EPT taxa). A higher presence of EPT taxa equates to better water quality because their water bound larvae assist in maintaining clean freshwater environments and will not survive in highly polluted water. Human disruption to natural environments is causing increases in polluted freshwater, leading to decreases in the presence of EPT taxa. Texas Parks and Wildlife Department documentation shows major concern for the populations of eight EPT species of …
Catfish Neurocranium 3-D Scan, Pamela Gonzalez, Jarod Johnson, David Kerstetter
Catfish Neurocranium 3-D Scan, Pamela Gonzalez, Jarod Johnson, David Kerstetter
All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series
No abstract provided.
Hypothalamic Manipulation Of The Anuran Hpg Axis: Alternative Hormones And A Non-Invasive Administration Route For Amphibian Assisted Reproductive Technologies (Art), Erin Michelle Saylor
Hypothalamic Manipulation Of The Anuran Hpg Axis: Alternative Hormones And A Non-Invasive Administration Route For Amphibian Assisted Reproductive Technologies (Art), Erin Michelle Saylor
Theses and Dissertations
Amidst the amphibian extinction crisis, in situ and ex situ amphibian species conservation initiatives utilize assisted reproductive technologies for optimal genetic management of captive and wild populations. Development of effective, simple, low-cost methods for obtaining gametes for artificial fertilization, sperm biobanking, or natural breeding alleviates obstacles for institutions or programs to apply reproductive technologies. Objectives herein include investigating the efficacy of alternative hormones for inducing spermiation, an alternative hormone administration route for inducing ovulation, exploring the physiological effects of hormone therapy, and utilizing sperm from deceased animals for biobanking.