Animal Sciences Commons^™

Evaluation Of Protective Efficacy Of Viral Vector Based Swine Influenza Vaccine And A Method For B Cell Culture For Monoclonal Antibody Generation, Sushmita Kumari

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Influenza A virus of the swine (IAV-S) is an economically important swine pathogen that has the potential to spread to humans, thus posing an ongoing public health concern due to its zoonotic potential. Hemagglutinin (HA), the most abundant viral envelope protein, is known to be the key protective antigen. Anti-HA antibodies alone, have been shown to prevent IAV infection. In this study we evaluated the feasibility of a recombinant tri segmented Pichinde virus (PICV) as a viral vector to deliver IAV-S hemagglutinin antigen in pigs. Four groups of weaned pigs (T01-04) were immunized twice with PBS, rPICV-GFP as a vector …

Aquatic Diversity In A Changing Tropical Andean Glacierized Catchment: Macroinvertebrates Reveal Possible Important Consequences To The Chimborazo Region As Glaciers Recede And The Climate Continues To Change, Tanner Thomas

Independent Study Project (ISP) Collection

Tropical Andean glaciers are retreating rapidly, and their disappearance will have drastic consequences for the people and ecosystems that depend on them. While they have begun to receive the attention they deserve in scientific literature, much is still not known. Majority of these investigations have taken place on the Volcán Antisana, while other important glacierized systems, like the Volcán Chimborazo have received little attention. This investigation aimed to a) evaluate the possible effects of glacier loss and climate change on aquatic ecosystems by comparing glacial meltwater, non-glacial and mixed streams, and b) to evaluate the effects of pasture intensity on …

Good Cop/Bad Cop: A Comparative Analysis Of Affiliative And Aggressive Behaviors By Adult Female And Male Olive Baboons With Infants At Randilen Wildlife Management Area, Tanzania, Jesse Elop

Independent Study Project (ISP) Collection

This study investigated the infant-directed behaviors of adult male and female olive baboons in Randilen Wildlife Management Area in April of 2022. The objectives were 1) To determine the difference between olive baboon males and females in frequency and duration of listed behaviors towards infants; 2) To classify the interactions of adult male and adult female olive baboons with infants as either primarily affiliative or primarily aggressive; 3) To determine the frequency of selected behaviors at different reproductive phases (lactation, estrus, neutral, indeterminant) amongst female olive baboons towards infants. A single baboon troop in Randilen Wildlife Management Area was observed …

Sacred Scavengers: Vulture Conservation In Nepal, Hans Nedde

Independent Study Project (ISP) Collection

In the 1990s, a veterinary drug used to treat pain and disease in cattle nearly brought the nine vulture species of Nepal to extinction. In a span of 15 years, over 97% of vultures in Nepal perished. For the past 20 years, governments, organizations, and communities have been working together to save these vital scavengers from vanishing. From the lowlands to the Himalayas, vultures have been interacting with the environment and humans for millennia. This study explores the role that vultures play both ecologically and culturally in Nepal. It investigates vultures as a crucial ecological influence and how human action …

Improving The Longevity Of Dairy Cattle: An Important Initiative For The Swiss Dairy Industry, Frances Mcmillan

Independent Study Project (ISP) Collection

Swiss dairy cattle rarely live a productive life of longer than 5 years- sometimes even less, and therefore fail to reach their maximum lactation potential. They are often prematurely culled due to udder diseases, fertility problems, lameness, or the farmer’s need to make room for a calving heifer. However, farmers rarely record the real reasons for removing a cow from the herd, due to a sense of pressure and obligation to produce and a lack of universal standards regarding longevity to assist in this decision. This is a monumental problem, from both an environmental and animal welfare point of view, …

Comparing Amphibian Species Diversity And Abundance In Natural Forest And Cacao Agroforest At Finca La Magnita, Changuinola, Bocas Del Toro, Panamá, Lake Barrett

Independent Study Project (ISP) Collection

Amphibians are currently undergoing rapid and drastic biodiversity loss worldwide, largely due to the disease Chytridiomycosis. Because of this, efforts to conserve amphibian biodiversity are urgent and have been given increasing importance. However, studies investigating the effectiveness of agroforestry systems, a commonly used agricultural method in which trees and other elements of forests are retained on land used for agriculture, for amphibian conservation are uncommon. As a result, the capacity of agroforests to serve as a tool for amphibian conservation is unclear. To determine if agroforests can serve as a habitat for amphibians and a tool for their conservation, this …

Ecological Studies Of Wolves On Isle Royale Annual Report 2021-2022, Sarah R. Hoy, Rolf O. Peterson, John A. Vucetich

Ecological Studies of Wolves on Isle Royale

Annual Report 2021-2022

Estimating Fish Diet In Lake Turkana, Kenya, Julia G. Sharapi

Student Publications

Lake Turkana, located in northern Kenya on the Ethiopian border, is the world’s largest permanent desert lake. Over 90% of its flow originates from the Omo River in Ethiopia, which causes the lake to have seasonal rises and falls in water level. The Omo River has been subject to the construction of five dams (the Gilgel Gibe dams) and several irrigation projects. These developments will impact the amount of water entering the lake, with implications for lake chemistry and productivity, littoral habitat availability, and the breeding biology of the lake’s fishes. Though ongoing upstream changes are likely to have serious …

Leveraging Land Easements For Grassland Bird Habitat Conservation, Amy N. Marigliano, Hayden E. Dubniczki, Sarah W. Westrick

Student Publications

In addressing the decline of North American grassland bird populations, it is important to consider the various interdisciplinary approaches that can be employed in their conservation. OECMs, or “other effective area-based conservation measures” encompass a wide array of strategies which can be leveraged to conserve natural landscapes and species. Land easements implemented by the Land Conservancy of Adams County (LCAC) are an example of one such strategy. The LCAC seeks primarily to preserve the rural character of Adams County but has more recently turned their focus toward environmental conservation. In partnering with the Land Conservancy, this case study aimed to …

The Impact Of Plant Cell Wall Lignin On Energy Utilization In Lactating Jersey Cows, Jason D. Stypinski

Department of Animal Science: Dissertations, Theses, and Student Research

Fiber from forages comprises a significant proportion of dairy cattle rations and by extension, it contributes largely to the energy concentration of the diet. While the proportion of fiber in the diet is important, the composition of fiber is probably more important as the different constituents of this fraction vary in their contributions to the nutritive value of the feed. Lignin has been described as an important factor limiting the digestion of NDF, reducing intake, and compromising milk production. Although lignin’s effects on these responses have been well characterized, the literature lacks data on the use of indirect calorimetry to …

Annotating Gene Expression And Regulatory Elements In Tissues From Healthy Thoroughbred Horses And Identifying Candidate Mutations Associated With Perosomus Elumbis In An Angus Calf, Alexa Mckenna Barber

Department of Animal Science: Dissertations, Theses, and Student Research

Genome annotation has a direct impact on the success of genomic studies. Transcriptome analyses and chromatin immunoprecipitation and sequencing (ChIP-seq) have been used to functionally annotate genomes. These methods can identify protein-coding genes, non-coding transcripts, and cis-regulatory elements across the genome. The primary objective of the first study was to functionally annotate the equine genome through the assessment of nine tissues: adipose, brain, heart, lamina, liver, lung, skeletal, muscle, testis, and ovary. In the first project, 150 bp, paired-end RNA sequencing (RNA-seq) libraries were generated in stallion tissues and compared to previously generated mare RNA-seq libraries to quantify variation in …

Effects Of Temperature And Plant And Animal Diets On Metabolic Rate In The Juvenile Red Eared Slider (Trachemys Scripta Elegans), Taylor Macdonald, Sarah Bouchard

Undergraduate Honors Thesis Projects

Specific dynamic action (SDA) is the energy expended during ingestion, digestion, absorption, and assimilation of a meal, and is influenced by meal (type, size, composition, and temperature) and environmental temperature. Understanding the effect of meal type and environmental temperature on SDA in turtles is important in describing how T. s. elegans may acclimate with changing environmental temperatures. In this study, we conducted feeding trials in which we fed juvenile T. s. elegans duckweed and mealworm diets at 25°C and 30°C. We measured the rate of oxygen consumption as a proxy for metabolic rate after feeding for four 30 minute consecutive …

Fragile Foal Syndrome: Its Past, Present, And Future, Alena Martin, Alena M. Martin

Honors College Theses

Though Fragile Foal Syndrome (FFS) has been around for some time, the public interest in FFS is recent. The hype spread rapidly from a public media announcement that a stallion at a prominent North American stud farm was a carrier (Brooks, 2021). As with most genetic mutations, FFS likely arose due to the increased inbreeding that results from human selection (Orlando & Librado, 2019). Fragile Foal Syndrome affects the enzyme responsible for developing the connective tissues. Two recent case studies highlighted the devastating effect of FFS on every part of the equine body (Metzger, et al., 2020; Grillos, 2021). So …

Genomic Analysis Of Metabolic Differences Found In Clostridium Perfringens That Cause Necrotic Enteritis In Poultry, Connor M. Aylor

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Clostridium perfringens is a common member of gut microbiota in healthy animals, but can also be an important pathogen in human and veterinary medicine. It produces several protein toxins that contribute to both histotoxic and enteric diseases in animals. Necrotic enteritis in poultry has been associated with the NetB toxin of C. perfringens; however, this toxin alone is insufficient to cause disease in infected chickens. While considerable research has focused on the presence of toxins and virulence factors, little has been done to assess the function of metabolic factors on the ability of the bacteria to cause disease. …

Sex-Specific Effects Of Hatching Order On Nestling Baseline Corticosterone In A Wild Songbird, Madison A. Rittinger, Rachel M. Bowden, Logan A. Sauers, Ryan T. Paitz, Christine J. Poppe, Charles F. Thompson, Scott K. Sakaluk

Faculty Publications – Biological Sciences

Variation in nestling growth and survival is often influenced by hatching order, with first-hatched offspring having an advantage over later-hatched younger siblings. In house wrens (Troglodytes aedon), this effect of hatching order is especially evident in asynchronously hatched broods and can lead to sex-specific differences in the size and condition of nestlings. Females appear to allocate the sex of their offspring across the laying order to capitalize on these differences. We hypothesized that levels of circulating corticosterone, the primary metabolic hormone in birds, mediates these sex-specific effects in nestlings. We predicted that: i) baseline levels of corticosterone in nestlings should …

Environment-Driven Shifts In Inter-Individual Variation And Phenotypic Integration Within Subnetworks Of The Mussel Transcriptome And Proteome, Richelle L. Tanner, Lani U. Gleason, W. Wesley Dowd

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

The environment can alter the magnitude of phenotypic variation among individuals, potentially influencing evolutionary trajectories. However, environmental influences on variation are complex and remain understudied. Populations in heterogeneous environments might exhibit more variation, the amount of variation could differ between benign and stressful conditions, and/or variation might manifest in different ways among stages of the gene-to-protein expression cascade or among physiological functions. Here, we explore these three issues by quantifying patterns of inter-individual variation in both transcript and protein expression levels among California mussels, Mytilus californianus Conrad. Mussels were exposed to five ecologically relevant treatments that varied in the mean …

3d Library From Body Size From Unconventional Specimens: A 3d Geometric Morphometrics Approach To Fishes From Ancestral Pueblo Contexts, Jonathan Dombrosky, Thomas F. Turner, Alexandra Harris, Emily Lena Jones

Anthropology Faculty & Staff Publications

Animal body size estimation from zooarchaeological specimens often relies on specific, one-dimensional (i.e., conventional) measures from skeletal elements. Here, we introduce an animal body size estimation technique for archaeological fishes that relies on 3D reference scans and the calculation of centroid size, a standard 3D geometric morphometric proxy measure for organism size. Centroid size-based estimations on whole caudal vertebrae are strongly correlated with a widely accepted measure (i.e., centrum width), but the scalability and flexibility of the centroid size-based approach allows for use on a wide variety of fragmented remains. We use zooarchaeological fish remains (subfamily Ictiobinae) from late pre-Hispanic …

European Starling Use Of Nest Boxes Relative To Human Disturbance, Bradley Blackwell, Bruce N. Buckingham, Morgan B. Pfeiffer

United States Department of Agriculture Wildlife Services: Staff Publications

gars and to engine and flight surfaces of moored aircraft. We questioned whether consistent removal of nest material would negatively affect use of a nest site, measured by a reduction in material accumulation. We conducted our study on a 2,200-ha site in Erie County, Ohio, USA (41° 22’ N, 82° 41’ W), from April 15 through June 2, 2020. We used 120 wooden nest boxes on utility poles, protected by an aluminum predator guard below the box. Our treatments included (1) twice weekly, repeated nest material removal (RMR; n = 40 nest boxes); (2) complete nest removal, but only after …

Understanding Continent-Wide Variation In Vulture Ranging Behavior To Assess Feasibility Of Vulture Safe Zones In Africa: Challenges And Possibilities, Adam Kane, Ara Monadjem, H. K.Ortwin Aschenborn, Keith Bildstein, André Botha, Claire Bracebridge, Evan R. Buechley, Ralph Buij, John P. Davies, Maria Diekmann, Colleen T. Downs, Nina Farwig, Toby Galligan, Gregory Kaltenecker, Chris Kelly, Ryno Kemp, Holger Kolberg, Monique L. Mackenzie, John Mendelsohn, Msafiri Mgumba, Ran Nathan, Aaron Nicholas, Darcy Ogada, Morgan Pfeiffer, W. Louis Phipps, Matteuns D. Pretorius, Sascha Rösner, Dana G. Schabo, Gabriel Lita Shatumbu, Orr Spiegel, Lindy J. Thompson, Jan A. Venter, Munir Virani, Kerri Wolter, Corinne J. Kendall

United States Department of Agriculture Wildlife Services: Staff Publications

Protected areas are intended as tools in reducing threats to wildlife and preserving habitat for their long-term population persistence. Studies on ranging behavior provide insight into the utility of protected areas. Vultures are one of the fastest declining groups of birds globally and are popular subjects for telemetry studies, but continent-wide studies are lacking. To address how vultures use space and identify the areas and location of possible vulture safe zones, we assess home range size and their overlap with protected areas by species, age, breeding status, season, and region using a large continent-wide telemetry datasets that includes 163 individuals …

Economic Consequences Of The Wolf Comeback In The Western United States, Dana Hoag, Stewart W. Breck, Kevin Crooks, Becky Niemiec

United States Department of Agriculture Wildlife Services: Staff Publications

Gray wolves were eradicated from most of the United States in the 1940’s but have made a comeback in parts of their historic range over the last two decades. First reintroduced into the Greater Yellowstone Ecosystem and central Idaho in the mid-1990’s, wolves have subsequently dispersed into at least 7 western states. Coloradoans became the latest state to take interest in bolstering wolf populations, as residents passed a ballot initiative in November 2020 to reintroduce a self-sustaining population of gray wolves by the end of 2023. Conflicts between people in rural areas that might incur costs (such as livestock loss) …

Ileal Microbial Composition In Genetically Distinct Chicken Lines Reared Under Normal Or High Ambient Temperatures, Nima K. Emami, Lori L. Schreier, Elizabeth Greene, Travis Tabler, Sara K. Orlowski, Nicholas B. Anthony, Monika Proszkowiec‑Weglarz, Sami Dridi

Poultry Science Faculty Publications and Presentations

Background: Heat stress (HS) has negative effects on poultry productivity, health and welfare resulting in economic losses. Broiler chickens are particularly susceptible to HS due to their high metabolic rate and rapid growth. The com‑ mensal intestinal bacterial populations have an important physiological role in the host and could ameliorate the negative effect of HS on the host. Thus, the aim of this study was to compare changes in the ileal (IL) microbiota in four different broiler lines during HS.

Results: Day‑old broiler chicks from Giant Jungle Fowl (JF), Athens Canadian Random Bred (ACRB), 1995 Random Bred (L1995), and Modern …

Quantitative Morphometric, Physiological, And Metabolic Characteristics Of Chickens And Mallards For Physiologically Based Kinetic Model Development, Colin G. Scanes, Johannes Witt, Stephan Schaller, Vanessa Baier, Audrey J. Bone, Thomas G. Preuss, David Heckmann

Poultry Science Faculty Publications and Presentations

Physiologically based kinetic (PBK) models are a promising tool for xenobiotic environmental risk assessment that could reduce animal testing by predicting in vivo exposure. PBK models for birds could further our understanding of species-specific sensitivities to xenobiotics, but would require species-specific parameterization. To this end, we summarize multiple major morphometric and physiological characteristics in chickens, particularly laying hens (Gallus gallus) and mallards (Anas platyrhynchos) in a meta-analysis of published data. Where such data did not exist, data are substituted from domesticated ducks (Anas platyrhynchos) and, in their absence, from chickens. The distribution of water …

Quantitative Comparison Of Avian And Mammalian Physiologies For Parameterization Of Physiologically Based Kinetic Models, Colin G. Scanes, Johannes Witt, Markus Ebeling, Stephan Schaller, Vanessa Baier, Audrey J. Bone, Thomas G. Preuss, David Heckmann

Poultry Science Faculty Publications and Presentations

Physiologically based kinetic (PBK) models facilitate chemical risk assessment by predicting in vivo exposure while reducing the need for animal testing. PBK models for mammals have seen significant progress, which has yet to be achieved for avian systems. Here, we quantitatively compare physiological, metabolic and anatomical characteristics between birds and mammals, with the aim of facilitating bird PBK model development. For some characteristics, there is considerable complementarity between avian and mammalian species with identical values for the following: blood hemoglobin and hemoglobin concentrations per unit erythrocyte volume together with relative weights of the liver, heart, and lungs. There are also …

Focused Review: The Role Of Drug Combinations For The Control Of Coccidiosis In Commercially Reared Chickens, H. David Chapman, Thilakar Rathinam

Poultry Science Faculty Publications and Presentations

A survey of drug combinations employed by the poultry industry indicates that they have played an important role in the control of coccidiosis in chickens. The mode of action of their component drugs is described. Advantages that accrue from their use may include a reduction in potential toxicity, a broadening of their spectrum of activity against different species of Eimeria, activity against different stages of the life cycle, and improved efficacy due to synergism between component drugs. Integration of management procedures involving rotation of drug combinations with vaccination is desirable because this has been shown to result in a restoration …

Avian Neuropeptide Y: Beyond Feed Intake Regulation, Elizabeth S. Greene, Nedra Abdelli, Jalila S. Dridi, Sami Dridi

Poultry Science Faculty Publications and Presentations

Neuropeptide Y (NPY) is one of the most abundant and ubiquitously expressed neuropeptides in both the central and peripheral nervous systems, and its regulatory effects on feed intake and appetite- have been extensively studied in a wide variety of animals, including mammalian and non-mammalian species. Indeed, NPY has been shown to be involved in the regulation of feed intake and energy homeostasis by exerting stimulatory effects on appetite and feeding behavior in several species including chickens, rabbits, rats and mouse. More recent studies have shown that this neuropeptide and its receptors are expressed in various peripheral tissues, including the thyroid, …

Monitoring The Immune Status Of Calves At The Agricultural Education Center, Jared Wallen

Honors Theses

Each year at the Andrews University Agriculture Education Center calves born to the resident cows and calves that are purchased are raised by the students in the animal science program. In each of the previous two years that calves have been raised, morbidities and mortalities have been observed. In order to prevent any calves from dying and reduce the rate of illness this research project was designed to monitor the calves’ immune status. By doing weekly blood draws, the total serum protein levels were determined and used as a standard for their health. Body temperature, food and water intake, and …

Evaluation Relativity Variation And Some Minerals Concentration During Lactation Stage Of Syrian Mountains Goat Milk Under Grazing System, تقدير التغيرات النسبية ومستوى تراكيز بعض المعادن خلال مرحلة إنتاج حليب الماعز الجبلي السوري تحت نظام الرعاية السرحي, Osama Diab, Samuel Mossa, Shehadeh Qaskos

Arab Journal of Arid Environments المجلة العربية للبيئات الجافة

The aim of this study was to investigate the effect of lactation stage on chemical composition and some minerals concentration of Syrian mountains Goat milk using grazing system in Al-Quneitera area (Syria) in year 2013. Twenty mountains Syrian goats were used to evaluate milk production and composition (fat, protein, lactose, and non-fat solids) and some minerals concentration (Ca, P, Na, K, Cu, Mg, Mn, Fe, Zn, cl) during lactation. Using SPSS program, data were analyzed by ANOVA with repeated measures. Daily milk yield was 1473.1 ± 297.42 g/head, while the total milk was 342.02 ± 135.6 Kg/head. The results of …

Estimation Of Growth Curve Prediction Equation Parameters For Weights Of Shami Camel Before Mature/ Syria, تقدير معالم دالة تنبؤ منحنى النمو لأوزان الإبل الشامية قبل النضج في سورية, Shadi Faris, Khaled Alnajjar, Ali Dib, Abdulla Nouh

Arab Journal of Arid Environments المجلة العربية للبيئات الجافة

The aim of this study was to estimate the growth curve prediction equation for weights from birth to four years old of Shami camel.

This study was carried out on 358 records of Shami camel, during the period from 1996 to 2012 at Dier Alhajr station for camel research, animal wealth research administration, General Commission Scientific Agriculture Research, rural Damascus (Syria).

The results showed high values of coefficient determinates (0.97-0.98), while standard errors values ranged between (27.26-34.13), and residual values ranged between (0.32-107.72), for each linear, quadratic and cubic equations. The values were fittest at linear regression equation for males, …

A Statistical Method For Quantifying Animal Interaction; Combining Time Geography And Simulation, Zachary J. Smith

USF Tampa Graduate Theses and Dissertations

Dynamic interaction (DI) describes the synchronous interaction of one or more individual animals over time. Current methods to quantify DI from tracking data are lacking. They typically require synchronized tracking data which limits their applicability to most studies. They also often do not have built-in tests of significance to evaluate interaction, and if so, rely on random walk models that lack biological realism. This research aimed to develop and evaluate a new way of measuring animal interaction that overcomes these limitations. The goal was to create a statistical method for quantifying and evaluating interaction that is relevant across computational, behavioural, …

High-Frequency Accelerometer Recording Of Key Predatory Behaviors In Vipers: Validation And Case Study With Timber Rattlesnakes (Crotalus Horridus), Morgan L. Thompson, Richard H. Adams, Anna Tipton, Dominic L. Desantis

Graduate Research Showcase

High-frequency accelerometer recording of key predatory behaviors in vipers: validation and case study with Timber Rattlesnakes (Crotalus horridus)

Morgan Thompson, Richard H. Adams, Anna F. Tipton, and Dominic L. DeSantis

Tri-axial accelerometers (ACTs) are becoming increasingly common in studies of animal behavior wherein direct observation of subjects in nature is constrained or impossible. ACTs are small (< 1 g) piezo-electric (spring-like) sensors that measure three-dimensional acceleration (upward, downward, and side-to-side) derived from subject motion. When leveraged with advanced machine learning techniques, these data can enable precise automated classification of a wide range of movement-mediated behaviors. Until recently, ACTs were largely reserved for larger-bodied organisms or those most amenable to the temporary external attachment of devices. Ongoing ACT miniaturization has now expanded the breadth of organisms amenable to these methods. This project aims to expand on a recently developed framework for ACT monitoring in wild-ranging snakes, a group that has been mostly overlooked in biologging applications. We are currently conducting extensive captive validation trials for robust model training and testing to enable classification of predatory behaviors, including striking and ingestion of prey items, in Timber Rattlesnakes (Crotalus horridus). Following captive validation, we will translate this method to the field with a population of C. horridus in the lower Piedmont of middle Georgia to evaluate the efficacy of externally attached ACTs for remote and continuous monitoring of …