Animal Sciences Commons^™

Comparing Pseudo-Environmental And Horizontal Plus Pseudo-Environmental Transmission Of A Probiotic Janithinobacterium Lividum Between Green Frog Tadpoles, Stephen Simonetti

Senior Honors Projects, 2010-2019

The gram-negative bacteria, Janthinobacterium lividum (J.liv) is able to kill the pathogenic fungus known as Batrachochytrium dendrobatidis (Bd), which is known to be causing massive amphibian decline worldwide. The purpose of this research was to identify the ability of the probiotic bacteria, Janthinobacterium lividum (J.liv), to be horizontally transmitted from individual to individual and/or pseudo-environmentally transmitted from individual to environment and back to individual between Lithobates clamitans (green frog) tadpoles. Based on previous findings I hypothesized that pseudo-environmental and horizontal transmission will occur in tadpoles. This would be important for helping stop the decline of amphibians because it would provide …

Distributional Expansion Of The Hispid Cotton Rat (Sigmodon Hispidus) Into Dawson County, Nebraska, Jennifer D. Frisch, Keith Geluso, Joseph T. Springer

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

In the last century, the Hispid Cotton Rat (Sigmodon hispidus) has moved northward in central parts of the United States, reaching Nebraska in the late 1950s. Recent surveys demonstrate Hispid Cotton Rats inhabit counties across southern parts of Nebraska south of the Platte River. Herein, we report on captures of S. hispidus from a new county (Dawson County), its seasonal occurrence at our study site, and the first known record of the Hispid Cotton Rat north of channels of the Platte River. Our captures represent one of the northern-most records in Nebraska.

Molecular Tools To Support Metabolic And Immune Function Research In The Guinea Fowl (Numida Meleagris), Carl E. Darris, James E. Tyus Ii, Gary Kelley, Alexander J. Ropelewski, Hugh B. Nicholas Jr., Xiaofei Wang, Samuel N. Nahashon

Agricultural and Environmental Sciences Faculty Research

Background

Guinea fowl (Numidia meleagris) production as an alternative source of meat and poultry has shown potential for economic viability. However, there has been little progress in characterizing the transcriptome of the guinea fowl. In this study RNA-sequencing and de novo transcriptome assembly of several Guinea fowl tissues (pancreas, hypothalamus, liver, bone marrow and bursa) which play key roles in regulating feed intake, satiety, and immune function was performed using Illumina’s Hi-Seq 2000.

Results

74 million sequences were generated and assembled into 96,492 contigs using the Trinity software suite. Over 39,000 of these transcripts were found to have …

Trypanosoma Cruzi Prevalence In The Domestic Canine Population In Central And Eastern Kentucky, Katelyn Cox

Mahurin Honors College Capstone Experience/Thesis Projects

Domestic canines are regarded as natural sentinelsfor the transmission of vector-borne pathogens since infection in an owner’s dog suggests the presence of the vector in or around the household. In collaboration with the University of Kentucky’s Veterinary Diagnostic Lab in Lexington, Kentucky, we have investigated the prevalence of Trypanosoma cruzi (the causative agent of Chagas disease) in canines from central and eastern Kentucky via a serological test, polymerase chain reaction (PCR), and DNA sequencing. In addition, Canine SNAP 4Dx plus tests (Idexx Laboratories, Inc.) were used to determine the prevalence of four other vector-borne pathogens: Ehrlichia canis/ewingii, Borrelia burgdorferi, Anaplasma …

Using Capture-Mark-Recapture Techniques To Estimate Detection Probabilities & Fidelity Of Expression For The Critically Endangered James Spinymussel (Pleurobema Collina)., Alaina C. Esposito

Masters Theses, 2010-2019

The critically endangered James Spinymussel (Pleurobema collina) is a species of freshwater mussel endemic to Virginia’s James and Dan River basins. In the last 20 years, P. collina has experienced a substantial decline in numbers and currently occupies approximately 10% of its original habitat; however, little information is known about this species to assist in conservation. A 230-meter reach of transitional habitat in Swift Run was selected for repeat observations to estimate detection probabilities using a Capture-Mark-Recapture framework. In June 2014, visual scouting began to locate and tag P. collina (including other mussels in the community) with PIT …

Competition And Habitat Quality Influence Age And Sex Distribution In Wintering Rusty Blackbirds, Claudia Mettke-Hofmann, Paul B. Hamel, Gerhard Hoffman, Theodore J. Zenzal Jr., Anne Pellegrini, Jennifer Malpass, Megan Garfinkel, Nathan Schiff, Russell Greenberg

Faculty Publications

Bird habitat quality is often inferred from species abundance measures during the breeding and non-breeding season and used for conservation management decisions. However, during the non-breeding season age and sex classes often occupy different habitats which suggest a need for more habitat-specific data. Rusty Blackbird (Euphagus carolinus) is a forested wetland specialist wintering in bottomland hardwood forests in the south-eastern U. S. and belongs to the most steeply declining songbirds in the U.S. Little information is available to support priority birds such as the Rusty Blackbird wintering in this threatened habitat. We assessed age and sex distribution and …

Effects Of The Developmental Environment On Oxidative Damage And Antioxidants In Red-Eared Slider Turtle ( Trachemys Scripta Elegans ) Hatchlings, Lisa Allison Treidel

Theses and Dissertations

Developmental environments influence many individual phenotypes. However, currently we have a limited understanding of how the developmental environment influences oxidative stress resistance phenotypes. Oxidative stress is defined as a physiological state during which the production of harmful free radicals exceeds the protective capabilities of antioxidants. Variations among adults in susceptibility to oxidative stress can have important consequences for life history strategies and fitness related traits. Our work was aimed at providing insight in to the role that the developmental environment plays on oxidative stress phenotypes in the model oviparous reptile the red-eared slider turtle (Trachemys scripta elegans). Here, we conducted …

Section Abstracts: Agriculture, Forestry And Aquaculture

Virginia Journal of Science

Abstracts of the Agriculture, Forestry and Aquaculture Section for the 93rd Annual Virginia Academy of Science Meeting, May 2015, at James Madison University

Variation In Avian Vocalizations During The Non-Breeding Season In Response To Traffic Noise, Amy I. Oden, Mary Bomberger Brown, Mark E. Burbach, James R. Brandle, John E. Quinn

School of Natural Resources: Faculty Publications

Low-frequency traffic noise that leads to acoustic masking of vocalizations may cause birds to alter the frequencies or other components of their vocalizations in order to be heard by conspecifics and others. Altering parts of a vocalization may result in poorer vocal performance or the message contained in the vocalization being received incorrectly. During the winters of 2011–2012 and 2012–2013, we recorded and measured the “chick-a-dee” call of Black-capped Chickadees (Poecile atricapillus) and the “po-ta-to-chip” call of American Goldfinches (Spinus tristis) to determine whether components of the calls produced in areas of high traffic noise and …

Habituation To Auditory Stimuli By Captive African Elephants (Loxodonta Africana), Sarah Elizabeth Goodyear

Masters Theses & Specialist Projects

Elephants are cognitive species that exhibit many types of learning. Associative, social, and insight learning have been investigated with elephants, but one of the simplest forms, habituation, has not. As an individual learns that a stimulus is neither harmful nor beneficial, it will decrease its response to the stimulus through the process of habituation. Elephants possess a well-developed sensory system and may habituate to stimuli that could be used for enrichment and/or management. The aim of this study was to examine the habituation process of elephants in response to repeated presentations of two auditory stimuli –buzzing by a disturbed beehive …

Kentucky Warbler (Vol. 91, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Identification Of Species In Ground Meat Products Sold On The U.S. Commercial Market Using Dna-Based Methods, Dawn Kane

Food Science (MS) Theses

Mislabeling of ground meat products is a form of food fraud that can lead to economic deception and interfere with dietary restrictions related to allergens or religious beliefs. In various parts of the world, including Ireland, Mexico and Turkey, high levels of meat mislabeling have been reported between 2000-2015. However, there is currently a lack of information regarding this practice in the United States. Therefore, the objective of this study was to test a variety of ground meat products sold on the U.S. commercial market for the presence of potential mislabeling. Forty-eight ground meat samples were purchased from online and …

Arkansas Broiler Enterprise Assessment Tool… Ar B.E.A.T, Christopher R. Sims

Poultry Science Undergraduate Honors Theses

Poultry operations require numerous variable inputs for the operation to run. Due to this it is extremely difficult for poultry growers to estimate the financial capacity of the operation. The poultry industry had evolved over the years from back yard non-commercial hobby farms to vertically integrated contract farms. These changes have made it extremely important that poultry growers fully understand the revenue, expenses, and income associated with the operation. Under the contract system, it is difficult for poultry growers to have a full understanding of and expectation for their projected expenses and revenues from the operation due to variability in …

Saccharomyces Boulardii As An Enteric Health Promoter In Broiler Chickens, Caroline Stephanie Philpot

Poultry Science Undergraduate Honors Theses

Saccharomyces boulardii is a yeast that has been shown to have a probiotic effect on the gut health of humans, ruminants, and swine by helping to control intestinal homeostasis, preventing pathogens from colonizing, promoting beneficial enzyme production, and by other mechanisms. This trial is designed to test whether the addition of S. boulardii to the diet improves the enteric health of broilers. Forty-eight test pens each containing fifteen birds, a water line, and a hanging feed pan were set up in a commercial broiler house from day eighteen to day thirty-six of the flock. The pens were evenly divided into …

Cellular Responses In Rous Sarcoma In Arkansas Rous Sarcoma Regressor And Progressor Chickens, Alyce Kallie Sullivan

Poultry Science Undergraduate Honors Theses

Immunology Rous sarcoma cell-mediated immunity

Avs Corner, May 2015, Casey Wheeler, Emily Cook, Clair Profilet, Karen Taylor, Tina Rowland, Johanna Johnson

AVS Corner

No abstract provided.

Fisheries Management Paper No 274 - Finfish Resources Of The Peel-Harvey Estuary - Harvest Strategy 2015-2020 (Version 1.0), Department Of Fisheries, Western Australia

Fisheries Management Papers

WEST COAST ESTUARINE MANAGED FISHERY (AREA 2)

Harvest strategies for aquatic resources managed by the Western Australian Department of Fisheries (the Department) are formal documents prepared to support the decision-making processes required to ensure the management of these resources are consistent with the principles of Ecologically Sustainable Development (ESD). The objectives of ESD are reflected in the objects of the Fish Resources Management Act 1994 (FRMA), Section 3, and the draft Aquatic Resources Management Bill 2013 (ARMB), Clause 9, which will replace the FRMA once enacted.

The publication of these strategies is intended to make the decision-making considerations and processes …

Multi-Level Adjustments In Singing Behavior By A Migratory Songbird In Response To Urbanization, Jacob Job

Dissertations

The spread of high-amplitude anthropogenic noise across natural landscapes is a serious issue for vocally communicating animals. Noise disrupts communication, which has implications for individual fitness and population persistence. In response, animals adjust their vocal signals with increasing noise. As the increase in noise has occurred on a relatively short time scale, evolutionary responses are likely not the primary cause of adjustments. Instead, short-term behavioral adjustments may drive population differences; however, such adjustments are currently not well understood. Moreover, noise is only one characteristic of urbanized landscapes and whether other characteristics, such as highly reverberant surfaces interact with noise to …

Dna Methylation Analysis Of Lin28a & Hand1 In Electrostimulated Genetically Unmodified Porcine Fibroblast Cells Grown In Vitro, Sara E. Calicchia

Undergraduate Honors Capstone Projects

Pluripotent (undifferentiated) cells are important for their applications in regenerative medicine. However, gene-based nuclear reprogramming of adult somatic cells is a slow and inefficient process, and poses some risk to recipient patients. Therefore, a major goal within the field of stem cell biology is to find a way to accomplish adult somatic cell de-differentiation using small chemical modulators of gene expression or other non-genomic mechanisms. Pilot studies in our laboratory have suggested that cells exposed to silver ions generated by the passage of a weak electrical current through a silver wire show morphological and gene expression changes reminiscent of de-differentiation. …

The Influence Of Invasive Plants On The Small Mammal Community In A Cold Desert, Trinity N. Smith

Undergraduate Honors Capstone Projects

Exotic invasive species can alter ecosystem health. Cheatgrass (Bromus tectorum), Russian thistle (Salsola kali) and tall tumble mustard (Sisymbrium altissimum) are widely distributed invasive plants occurring throughout desert and shrub-steppe communities in the western United States. Due to the relative ease of capture, small mammal community metrics are often used to quantify overall ecosystem health. Studies examining small mammal communities are numerous but few have specifically examined the effects of invasive plants at the community level in arid ecosystems. In this study I examined community level small mammal responses to changes in microhabitat features, …

Effects Of Beef Finishing Diets And Muscle Type On Meat Quality, Fatty Acids And Volatile Compounds, Arkopriya Chail

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Consumer evaluation, proximate data, Warner-Bratzler shear force (WBSF), fatty acid (FA) composition and volatile compounds were analyzed from the ribeye steaks (LT) finished on conventional feedlot (FL) and forages, including a perennial legume, birdsfoot trefoil (BFT; Lotus corniculatus), and a grass, meadow brome (Bromus riparius Rehmann, Grass). Representative retail forage (USDA Certified Organic Grass-fed, COGF) and conventional beef (USDA Top Choice, TC) were investigated (n = 6) for LT. Additionally, the effects of diet on round (GM) and chuck (TB) muscles FL, BFT and Grass were explored. Forage-finished beef was less liked in most of the affected …

Development Of Genetic Goat And Hamster Models Of Atrial Fibrillation And Long Qt Syndrome; And Genetic Hamster Models Of Middle East Respiratory Syndrome, Dane A. Rasmussen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Atrial fibrillation (AF) and long QT syndrome (LQTS) are potentially lethal heart rhythm disorders that can be caused by mutations in the potassium channel gene KCNQ1. Middle East Respiratory Syndrome (MERS) is a viral infection with the potential to replicate the devastating effects of the SARS outbreak in 2003. All three of these diseases are in need of genetic animal models.

To address these needs, my thesis project focused on the development of genetic goat and hamster models of AF and LQTS, and genetic hamster models of MERS. Because of the goat’s similar organ size/physiology and the hamster’s similar lipid …

Growth Performance, Ruminal Fermentation Characteristics, And Economic Returns Of Growing Beef Steers Fed Brown Midrib, Corn, Silage-Based Diet, Christopher Scott Saunders

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Sustainable beef production is extremely important to the beef cattle industry. Sustainability influences the environment, overall profits, and public concerns. One
factor that influences sustainability is the composition of cattle feed. This study compared conventional corn silage (CCS), which is most commonly used in beef steer feed, to brown midrib corn silage (BMR). Steers fed the two different diets were compared to determine differences in the areas of growth/animal performance, ruminal fermentation (digestion), and economic returns. The study included 24 beef steers randomly assigned to one of two treatment groups. Treatment groups were a total mixed feed ration that included …

Improvement Of Nutrient Utilization Efficiency, Ruminal Fermentation And Lactational Performance Of Dairy Cows By Feeding Birdsfoot Trefoil, Rachael G. Christensen

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Condensed tannins (CT) are compounds that have shown potential to reduce the environmental impact of dairy farming waste products. In two live animal studies and a continuous culture study, it was hypothesized that feeding birdsfoot trefoil (Lotus corniculatus, BFT), a CT-containing legume, would improve nutrient utilization, milk, and component yield of dairy cows compared with feeding alfalfa hay or grass pasture in two studies, while feeding BFT forage would decrease methane production and improve rumen fermentation in addition to concentrate supplementation was the hypothesis of the third study. Reduction in milk urea nitrogen (MUN) and …

Effect Of A Trace Mineral Injection On Beef Cattle Performance, Carmen J. Brasche

Department of Animal Science: Dissertations, Theses, and Student Research

Adequate trace mineral status is important in beef cow/calf and replacement heifer systems because trace minerals play vital roles in reproduction, immunity and growth. Many experiments have investigated the use of a trace mineral injection containing Cu, Mn, Se and Zn (TMI) on growth and immunity of beef cattle. However, very few have investigated TMI use on reproductive performance. Therefore, five experiments, a two year cow/calf experiment, and four replacement heifer experiments were designed to investigate the use of a TMI on reproductive performance of cows and heifers, calf growth and trace mineral status of cows, heifers, and calves. The …

Corn Plant Maturity Effect On Yield And Nutritional Quality: Corn Silage Inoculation On Performance Of Cattle Fed Silage With Or Without Live Yeast Added, Cassandra A. Row

Department of Animal Science: Dissertations, Theses, and Student Research

Corn plots were serially harvested over two years to evaluate nutrient, digestibility, and yield change as plants matured from half-milk line through black layer. In yr 1 (2013), two corn plots were used, one short season (102 d), and one normal season (111 d). Year two used two Pioneer hybrids (111 d; 112 d). Silage yield and silage DM increased quadratically as maturity increased (P ≤ 0.01). Percent NDF increased quadratically (P = 0.01), and NDF-digestibility decreased linearly (P < 0.01) as maturity increased. This study suggests there is a delicate balance between plant maturity, nutrient content, and silage yield. A finishing study using 320 yearling steers (initial BW = 417 ± 22.7 kg) evaluated the effect of using a silage inoculant or not on performance and carcass characteristics. Treatments were designed as a 2×2×2 factorial arrangement with factors being no inoculant (CON) or use of inoculant (Buchnerii spp.; B500) at silage harvest, silage fed at 15 or 40% of diet DM, and presence (LEV) or absence (noLEV) of Levucell SC yeast product fed at 14.2 g/hd/d. Performance data were analyzed with pen as the experimental unit, with 5 pens/treatment. There was a three-way interaction for final live BW, HCW, ADG, and G:F (P < 0.05). Feeding corn silage at 40% inclusion instead of 15% inclusion increased DMI and decreased G:F. At 15% silage inclusion, the B500 LEV treatment had the lowest ADG. At 40% silage inclusion all treatments were similar for ADG (P ≥ 0.06). When including silage at 15%, using B500 inoculant, the addition of LEV did not …

Impact Of Wet Distillers' Grains Plus Solubles And Antioxidants On A Basic Mechanism Of Beef Tenderization, Michael D. Chao

Department of Animal Science: Dissertations, Theses, and Student Research

Feeding high levels of wet distillers grains plus solubles (WDGS) increases polyunsaturated fatty acid (PUFA) levels in beef. Perhaps, WDGS in feedlot diets increases PUFA concentration in the SR membrane, thereby altering membrane integrity, resulting in more rapid calcium leakage and improved tenderness. Feeding antioxidants may mitigate such effects. In the first study, effects of feeding 50 % WDGS on SR membrane composition, free calcium concentration and tenderness were studied. The SR membrane from steers fed WDGS were more tender, had higher free calcium concentration, had more PUFA, more phosphatidylcholine (PC), less phosphatidylethanolamine (PE) and less total phospholipids when compared …

Use Of Endocrine And Immune Responses As Predictors Of Bull Sperm Motility, Lydia Mitchener

Animal Science Undergraduate Honors Theses

Research has shown that immune cells increased from an immune response, and endocrine concentrations directly affect sperm characteristics (Jones and Mann, 1976; Hansson et al., 1989; Grattan et al., 2007). Previous findings show a negative relationship between leukocytes and sperm function (Jones and Mann, 1976) and prolactin and fertility (Grattan et al., 2007). On the other hand, research has shown a positive relationship between insulin-like growth factor (IGF) and sperm characteristics (Hansson et al., 1989). The objective of this study is to identify biomarkers for yearling bull sperm associated with endocrine response and activation of the immune system. Seventeen Brahman-influenced …

The Role Of Rfamide-Related Peptide 3 (Rfrp3) In Regulation Of The Neuroendocrine Reproductive And Growth Axes Of The Boar, Jennifer F. Thorson, Amy T. Desaulniers, Chanho Lee, Brett R. White, J. Joe Ford, Clay A. Lents

Department of Animal Science: Faculty Publications

RFamide-related peptide 3 (RFRP3) has been implicated in regulating reproduction and growth. This regulation appears to be dependent upon sex, species, physiological status, and developmental stage. The objective of the present study was to evaluate the effects of RFRP3 on circulating concentrations of luteinizing hormone (LH) and growth hormone (GH) in mature boars. The hypothesis was RFRP3 would reduce circulating concentrations of LH and increase concentrations of GH. Meishan boars (716.6±2.8 days of age; 125.0±12.4kg BW) were randomly assigned to treatment: saline (n=4) or RFRP3 (8.5mg; n=5). Plasma was collected at 15-min intervals during 3 periods: pre-treatment, treatment, and post-treatment. …

Examination Of Polymorphism In The Serine Proteinase Inhibitor Gene In The Eastern Oyster, Crassostrea Virginica., Aine Lehane

Senior Honors Projects

Oysters are a keystone species; they are filter-feeders and reef-builders. Additionally, oysters are extremely important within the shellfish aquaculture industry; they are the second most widely produced species in world aquaculture production. Eastern oysters, Crassostrea virginica, are produced in aquaculture and are unfortunately susceptible to Dermo disease. Dermo is caused by a protozoan parasite, Perkinsus marinus, and infection is often fatal in oysters due to widespread tissue damage and blockage of blood vessels. Research in oyster disease is particularly needed because disease is one of the largest impediments faced by the aquaculture industry as entire oyster populations can …