Animal Sciences Commons^™

The Effects Of Hmtba (2-Hydroxy-4-Methylthio-Butanoic Acid) Supplementation On Ruminal Microbial Crude Protein Synthesis And Community Structure In Dairy Cattle, Chad James Robert Jenkins

Department of Animal Science: Dissertations, Theses, and Student Research

Metabolizable protein (MP) is protein that reaches the small intestine and is available for absorption and utilization by the cow. Dairy rations may be limited in the supply of MP essential to meeting the demands of milk synthesis, however as much as half of the MP flowing to the small intestine may be attributed to microbial origins and is referred to as microbial CP (MCP). Experiment 1 utilized a technique in which DNA was used as a microbial marker to estimate the concentration of bacterial CP (BCP) in the solid and liquid portions of rumen digesta. Rumen digesta was sampled …

The Fat Of The Matter: How Dietary Fatty Acids Can Affect Exercise Performance, Barbara J. Pierce, Scott R. Mcwilliams

Biology Faculty Publications

Fatty-acid composition of fat stores affects exercise performance in a variety of vertebrates although few such studies focus on flying vertebrates such as migratory birds, which are exceptional exercisers. We first discuss the natural variation in quality of fat available in natural foods eaten by migratory birds and their behavioral preferences for specific fatty acids in these foods. We then outline three proposed hypotheses for how dietary fatty acids can affect exercise performance, and some of the evidence to date that pertains to these hypotheses with special emphasis on the exercise performance of migratory birds. In theory, selectively feeding on …

News Of The Department Of Animal Science, Department Of Animal Science

Animal Science Newsletters

No abstract provided.

Documentation Of The Black-Footed Ferret, Mustela Nigripes On The Standing Rock Indian Reservation, Mobridge, South Dakota, Michael P. Gutzmer, Jeffrey C. Kelly

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Since 1991, 19 specific black-footed ferret reintroduction projects have been conducted across 8 States, Canada, and Mexico. All five of the first ferret reintroductions (from 1991 to 1996) continue to be occupied by ferrets. The photographs of October 31 and November 1 are the first unequivocal documentation of black-footed ferrets on the Standing Rock Indian Reservation. Ferrets observed on Standing Rock may be dispersals from the nearby Cheyenne River Sioux Indian Reservation to the south. The nearest re-introduction site for ferrets is approximately 20 miles away, in Whitehorse, South Dakota, and the observation of ferrets on Standing Rock could document …

Landscape Genetics Of The Endangered California Tiger Salamander (Ambystoma Californiense) In The Los Vaqueros Watershed, Ryan Neal Vincent

Mahurin Honors College Capstone Experience/Thesis Projects

Genetic diversity is essential for maintaining healthy biological systems, and dispersal-mediated gene flow increases genetic diversity by introducing new alleles to a gene pool. Populations that frequently experience gene flow will show characteristic similarities in allele frequencies. Measuring genetic diversity among many populations across a landscape has proven to be a powerful approach for assessing the ways that habitat discontinuities may affect patterns of gene flow, and in turn, influence allele frequencies and population dynamics. This study examines the landscape-level population genetic structure of the California tiger salamander, Ambystoma californiense, within the Los Vaqueros Watershed of Contra Costa County, …

Volatile Organic Compound Flux From Manure Of Cattle Fed Diets Differing In Grain Processing Method And Co-Product Inclusion, Kristin Hales, David B. Parker, N. Andy Cole

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Odor emissions from livestock production have become increasingly important in the past decade. Odors derived from animal feeding operations are caused by odorous VOC emitted from the mixture of feces and urine, as well as feed and silage which may be experiencing microbial fermentation. Distillers grains are a by-product of corn grain fermentation used to produce fuel ethanol, and this industry has grown rapidly throughout the U.S. in past years. Therefore, the use of wet distillers grains with solubles (WDGS) in feedlot cattle diets has also increased. The objective of this research was to determine specific VOC emissions from feces …

The Status Of Fishes In The Missouri River, Nebraska: Lake Sturgeon (Acipenser Fulvescens), Kirk D. Steffensen, Sam Stukel, Dane A. Shuman

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Lake Sturgeon (Acipenser fulvescens) occurrences in the Missouri River along Nebraska’s eastern border are historically sporadic and rare. Presently, the wild Lake Sturgeon population in this river reach may be extirpated. A Recovery Program initiated by the Missouri Department of Conservation (MDC) has stocked almost 150,000 hatchery-reared Lake Sturgeon into the lower Missouri River at several sites in Missouri. As a result, the number of Lake Sturgeon collected has increased. Since monitoring began in 2003, no Lake Sturgeon have been collected above Gavins Point Dam while 40 fish were collected downstream of Gavins Point Dam. The majority of …

The Figure Of Bitu-Man, Paul Royster

University of Nebraska-Lincoln Libraries: Faculty Publications

The ominously imposing figure in the above illustration is “the Syncrude bird deterrent device, locally known as ‘Bitu-man’.” He stands (or stood—the original image was from the late 1970s) in the tailings pond of the Syncrude Canada Ltd. oil sands mining operation along the Athabasca River near Fort McMurray in northeastern Alberta. That operation surface mines (i.e., strip mines) bitumen-impregnated sand, processes it with hot water and steam, and discharges the liquid effluent or “tailings” into a pond covering roughly 3000 hectares (11 square miles). (Google Map coordinates: 56.9°N, -111.3° W). Residue bitumen collects on the surface and poses a …

Response Surface Optimization Of Microwave-Assisted Sulfated Polysaccharide Extraction From Porphyra Dentate, Yeu-Pyng Lin, Shao-Chi Wu, Jean-Yu Hwang

Journal of Marine Science and Technology–Taiwan

An experiment was performed by adjusting the pH value (4-10) of a 10%-to-90% ethanol solution (v/v) and using it as an extraction solvent and, subsequently, using microwaveassisted extraction (MAE) to extract Porphyra denate solutions. The sulfated polysaccharides (µg/mL) of these extraction solutions were evaluated. The optimal treatments of P. denate extracts obtained using MAE were studied using response surface methodology. The study was performed using a three-level, four-factor design and aimed to determine the optimal combinations of the ethanol concentration (10%-90%, v/v, X1), pH value of an ethanol solution (4-10, X2), microwave power level (200-400 W, X3), and heating time …

Development Of A Methodology To Measure The Effect Of Ergot Alkaloids On Forestomach Motility Using Real-Time Wireless Telemetry, Amanda M. Egert, James L. Klotz, Kyle R. Mcleod, David L. Harmon

Animal and Food Sciences Faculty Publications

The objectives of these experiments were to characterize rumen motility patterns of cattle fed once daily using a real-time wireless telemetry system, determine when to measure rumen motility with this system, and determine the effect of ruminal dosing of ergot alkaloids on rumen motility. Ruminally cannulated Holstein steers (n = 8) were fed a basal diet of alfalfa cubes once daily. Rumen motility was measured by monitoring real-time pressure changes within the rumen using wireless telemetry and pressure transducers. Experiment 1 consisted of three 24-h rumen pressure collections beginning immediately after feeding. Data were recorded, stored, and analyzed using …

Efficient Gene Targeting In Golden Syrian Hamsters By The Crispr/Cas9 System, Zhiqiang Fan, Wei Li, Sang R. Lee, Qinggang Meng, Tom D. Bunch, Kenneth L. White, Zhongde Wang, Il-Keun Kong

Animal, Dairy, and Veterinary Science Faculty Publications

The golden Syrian hamster is the model of choice or the only rodent model for studying many human diseases. However, the lack of gene targeting tools in hamsters severely limits their use in biomedical research. Here, we report the first successful application of the CRISPR/Cas9 system to efficiently conduct gene targeting in hamsters. We designed five synthetic single-guide RNAs (sgRNAs)—three for targeting the coding sequences for different functional domains of the hamster STAT2 protein, one for KCNQ1, and one for PPP1R12C—and demonstrated that the CRISPR/Cas9 system is highly efficient in introducing site-specific mutations in hamster somatic cells. We …

Fish Cognition, Redouan Bshary, Culum Brown

Social Cognition Collection

No abstract provided.

Historical Vegetation Of Three Salmon-Bearing Watersheds In The Interior Columbia River Basin, Tyanna Smith

PSU McNair Scholars Online Journal

Land use practices can be a contributing factor to environmental degradation and have been the focus of many ecological studies. One aspect that is less addressed is land use history and the effects that past practices, such as logging and grazing, can have on the current landscape. This paper describes research and the synthesis of material on the environmental history and watershed characteristics for three watersheds located within spawning and rearing areas for Chinook salmon in the Grande Ronde River Basin in Northeast Oregon: upper Grande Ronde River, Catherine Creek, and Minam River. The Grande Ronde Basin is critical spawning …

Breeding Time In A Migratory Songbird Is Predicted By Drought Severity And Group Size, Charles R. Brown, Mary Bomberger Brown

School of Natural Resources: Faculty Publications

Global climate change is altering the breeding phenology of many organisms, and one reported consequence of warmer average temperatures is earlier breeding times in migratory songbirds of north temperate latitudes. Less studied are the potential interactions between earlier breeding and social behavior in colonial species. We investigated how breeding time, as measured by colony initiation dates across the entire summer, in Cliff Swallows (Petrochelidon pyrrhonota) of southwestern Nebraska, USA, changed over a 30-year period and could be predicted by climatic variables, year, and colony size. Mean colony initiation date became earlier over the study, with variation best predicted …

Economic Contribution Of Agriculture And Food To Arkansas' Gross Domestic Product 1997-2012, Leah English, Jennie Popp, Wayne Miller

AAES Research Reports and Research Bulletins

Agricultural production, processing, and retail industries are major contributors to the Arkansas economy in terms of GDP. Agriculture contributes to the economy through direct agricultural production, value-added processing, and agricultural retail activities, and it also plays an important role through its interactions with other sectors. The use of non-agricultural goods and services as inputs into the agricultural sector promotes diversified growth in Arkansas’ economy; thus agriculture remains a vital part of Arkansas’ economy. This report: 1) compares the relative size of the Agriculture and Food Sector in Arkansas with those of neighboring states, the Southeastern region of the United States, …

Fisheries Research Report No. 258 - Assessment Of Blue Swimmer Crab Recruitment And Breeding Stock Levels In The Peel-Harvey Estuary And Status Of The Mandurah To Bunbury Developing Crab Fishery, D Johnston, A Chandrapavan, B Wise, N Caputi

Fisheries Research Reports

Two projects were funded to investigate iconic blue swimmer crab stocks in the Peel-Harvey Estuary and Mandurah to Bunbury Developing Crab Fishery. One was a four year project (2007 – 2011) to undertake the following: i) determine recruitment and spawning stock levels of the crab population in the Peel-Harvey Estuary and whether the status of stocks has changed considerably in the past decade, ii) establish a commercial monitoring program to assess the length frequency and sex ratio of crabs captured by commercial fishers, and iii) develop a commercial monitoring program in the Mandurah to Bunbury Developing Crab Fishery and South …

Growing South Dakota (Fall 2014), College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

[Page] 2 Abundant Opportunities: Bright Ag & Bio Career Outlook Fuels Enhancements
[Page] 4 Future Focus: New Institute Supports Efforts In Science, Technology, Engineering, Math
[Page] 7 Grant Funding Updates
[Page] 8 Profiles in Leadership: Engaging Students Utilizing Problem-Based Learning
[Page] 9 Profiles in Leadership: The Value Of Experience-Based Learning
[Page] 10 Campus News
[Page] 12 Steps To Ensure Student Success: Support Fostered In Living-Learning Communities
[Page] 14 New Student Advising Model Gets High Marks
[Page] 15 Student To Student: Ag Bio Ambassadors Relish Role As Front Line Recruiters
[Page] 16 Remembering Dr. Robert Pengra: SDSU Alums Establish Tribute Scholarship …

Editorial Overview: Environmental Physiology: Insect Environmental Physiology, Brent J. Sinclair

Biology Publications

No abstract provided.

Adipogenic And Myogenic Stem Cells In Brown Fat: A Study Of Progenitors And Regenerative Capacity, Xin Yang

Open Access Theses

Brown adipose tissue (BAT) utilizes stored lipids to generate heat, therefore reducing body fat content and favoring a leaner body composition. Recent identification of metabolically active BAT in adult humans has sparked broad interests in understanding the developmental origin and postnatal homeostasis of BAT. However, the stem cell population that gives rise to BAT during development and maintains BAT mass at postnatal stage has not been characterized. In addition, whether adult BAT has the capacity to regenerate after injury or to proliferate in response to cold is flargely unknown. Furthermore, although BAT and skeletal muscle are known to share a …

Altered Theca And Cumulus Oocyte Complex Gene Expression, Follicular Arrest And Reduced Fertility In Cows With Dominant Follicle Follicular Fluid Androgen Excess, Adam F. Summers, William E. Pohlmeier, Kevin M. Sargent, Brizett D. Cole, Rebecca J. Vinton, Scott G. Kurz, Renee M. Mcfee, Robert A. Cushman, Andrea S. Cupp, Jennifer R. Wood

Department of Animal Science: Faculty Publications

Aspiration of bovine follicles 12–36 hours after induced corpus luteum lysis serendipitously identified two populations of cows, one with High androstenedione (A4; >40 ng/ml; mean = 102) and another with Low A4 (/ml; mean = 9) in follicular fluid. We hypothesized that the steroid excess in follicular fluid of dominant follicles in High A4 cows would result in reduced fertility through altered follicle development and oocyte maternal RNA abundance. To test this hypothesis, estrous cycles of cows were synchronized and ovariectomy was performed 36 hours later. HPLC MS/MS analysis of follicular fluid showed increased dehydroepiandrosterone (6-fold), A4 (158-fold) and testosterone …

Dangerous Mating Systems: Signal Complexity, Signal Content And Neural Capacity In Spiders, Marie E. Herberstein, Anne E. Wignall, Eileen Hebets, Jutta M. Schneider

Eileen Hebets Publications

Spiders are highly efficient predators in possession of exquisite sensory capacities for ambushing prey, combined with machinery for launching rapid and determined attacks. As a consequence, any sexually motivated approach carries a risk of ending up as prey rather than as a mate. Sexual selection has shaped courtship to effectively communicate the presence, identity, motivation and/or quality of potential mates, which help ameliorate these risks. Spiders communicate this information via several sensory channels, including mechanical (e.g. vibrational), visual and/or chemical, with examples of multimodal signaling beginning to emerge in the literature. The diverse environments that spiders inhabit have further shaped …

A Comparison Of Techniques Measuring Stress In Birds, Deborah A. O'Dell, Michael A. Carlo, Abigail Kimmitt, Ellen Bikowski, Katherine R. Morris, Andrew Dolby

Virginia Journal of Science

Free-living birds are subjected to both external and internal stresses which can affect their health, activity, and reproductive success. To study stress in free living birds, they must be captured in nets and handled by the researcher to take blood samples for commonly used measures of stress, an activity which itself can induce stress and confound results. This study compares the effects of handling time on three different measures of stress: levels of the stress hormone corticosterone (CORT), levels of Heat Shock Protein 60 (HSP 60) and the ratio of heterophils to lymphocytes (H/L ratio) in tufted titmouse (Baeolophus bicolor …

Kernel-Based Whole-Genome Prediction Of Complex Traits: A Review, Gota Morota, Daniel Penagaricano

Department of Animal Science: Faculty Publications

Prediction of genetic values has been a focus of applied quantitative genetics since the beginning of the 20th century, with renewed interest following the advent of the era of whole genome-enabled prediction. Opportunities offered by the emergence of high-dimensional genomic data fueled by post-Sanger sequencing technologies, especially molecular markers, have driven researchers to extend Ronald Fisher and Sewall Wright’s models to confront new challenges. Inparticular, kernel methods are gaining consideration as a regression method of choice for genome-enabled prediction. Complex traits are presumably influenced by many genomic regions working in concert with others (clearly so when considering pathways), thus generating …

The Role Of Adiponectin And The Adipocyte In Energy Metabolism And Inflammation, Sheila Kay Jacobi

Open Access Dissertations

A series of experiments were conducted to characterize the autocrine role of adiponectin in modulating fatty acid metabolism and inflammation in the pig. In the first study, we cloned and sequenced the porcine adiponectin open reading frame and evaluated the regulation of adiponectin, in vitro and in vivo. The porcine sequence shares approximately 88, 86, 85 and 83% homology with the dog, human, cow and mouse adiponectin, respectively, and 79–83% similarity with dog, human, cow and mouse proteins at the amino acid level, based on the translated porcine sequence and GenBank submissions for the other species. Analysis of serum from …

Multimodal Sensory Reliance In The Nocturnal Homing Of The Amblypygid Phrynus Pseudoparvulus (Class Arachnida, Order Amblypygi)?, Eileen A. Hebets, Alfonso Aceves-Aparicio, Samuel Aguilar-Argüello, Verner P. Bingman, Ignacio Escalante, Eben J. Gering, David R. Nelson, Jennifer Rivera, José Ángel Sánchez-Ruiz, Laura Segura-Hernández, Virginia Settepani, Daniel D. Wiegmann, Jay A. Stafstrom

Eileen Hebets Publications

Like many other nocturnal arthropods, the amblypygid Phrynus pseudoparvulus is capable of homing. The environment through which these predators navigate is a dense and heterogeneous tropical forest understory and the mechanism(s) underlying their putatively complex navigational abilities are presently unknown. This study explores the sensory inputs that might facilitate nocturnal navigation in the amblypygid P. pseudoparvulus. Specifically, we use sensory system manipulations in conjunction with field displacements to examine the potential involvement of multimodal—olfactory and visual—stimuli in P. pseudoparvulus’ homing behavior. In a first experiment, we deprived individuals of their olfactory capacity and displaced them to the opposite side of …

Ad Hoc Smoothing Parameter Performance In Kernel Estimates Of Gps-Derived Home Ranges, Krysten L. Schuler, Greg M. Schroeder, Jonathan A. Jenks, John G. Kie

Natural Resource Management Faculty Publications

Accuracy of home-range estimates in animals is influenced by a variety of factors, such as method of analysis and number of locations, but animal space use is less often considered and frequently over-generalized through simulations. Our objective was to assess effect of an ad hoc (h_ad hoc)smoothing parameter in kernel analysis from two species that were predicted to have different patterns of utilization distributions across a range of sample sizes. We evaluated variation in home-range estimates with location data collected from GPS collars on two species: mule deer Odocoileus hemionus and coyotes Canis latrans. We calculated home ranges using …

Regulatory Capture, The Chesapeake Bay, And Hampton Roads: What's On Your Plate, Ronnie David Gannon

Institute for the Humanities Theses

The Chesapeake Bay and its 64,000 square mile watershed are both severely impacted from excessive amounts of nutrient pollution, which contributes to a growing presence of dead zones in the Bay. What causes nutrient pollution? What are dead zones? In addition, many of the Bay's commercially valuable species of marine life have been/ are overexploited to the point of collapse / verge of collapse. Despite all of these facts, management agencies continue to weakly enforce regulations. Why?

Not to mention, much of the Bay's marine life is unsustainably caught in the contaminated waters of Hampton Roads. How, and for what? …

Predictors Patterns And Processes Of Extinction Risk In Porgies And Seabreams (Family: Sparidae), Mia Theresa B. Conteros

Biological Sciences Theses & Dissertations

A variety of human-induced changes are having profound impacts on the marine environment, and no area on the planet remains unaffected by the detrimental effects of our activities. These stressors can potentially lead to synergistic effects, causing accelerated biodiversity loss and diminished ecosystem functioning. Identification and understanding of the factors that drive species to heightened risk of extinction are important goals in conservation.

The Sparidae are commercially important and ecologically complex marine fishes; global extinction risk assessments using the IUCN Red List of Threatened Species methodology show that 9% (13 species) have increased vulnerability to population declines from intense fishing …

The Effects Of Heat Stress On Immunity In Laying Hens And Dairy Cattle, Rebecca Ann Strong

Open Access Theses

With the increase in global climate change and the population growth driving the high demand for additional food production, heat stress (HS) is a major concern in the livestock industry across all species. Animals experience HS when exposed to high environmental temperatures outside their thermal neutral zone. The level of the effects can vary due to the length and intensity of HS to which the animal is exposed to. In experiment one, laying hens with access to cooled perches during HS had a lower heterophil to lymphocyte ratio compared to the control hens after 4 h of acute heat stress, …

Prediction Of Parturition And Dystocia In Holstein-Friesian Cattle, And Cesarean Section In Dystocic Beef Cattle, Wen Han Mark Hiew

Open Access Dissertations

Dystocia is a major problem in the dairy industry as it causes livestock and economic loss. It is more frequently seen in primiparous cattle compared to their multiparous counterparts due to their smaller stature and the slow maturation of pelvic dimensions. In some instances, human intervention of the parturition process is imperative to avoid pain, injury, and mortality of the neonate and the dam. The ability to accurately predict dystocia and the time of parturition will ensure that timely assistance can be given to animals that are of high risk of dystocia.^ The present thesis contains four studies that explore …