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Articles 451 - 480 of 1049
Full-Text Articles in Animal Sciences
Polychaetes As Annelid Models To Study Ecoimmunology Of Marine Organisms, Virginie Cuvillier-Hot, Céline Boidin-Wichlacz, Aurélie Tasiemski
Polychaetes As Annelid Models To Study Ecoimmunology Of Marine Organisms, Virginie Cuvillier-Hot, Céline Boidin-Wichlacz, Aurélie Tasiemski
Journal of Marine Science and Technology–Taiwan
Annelids are among the first coelomates and are therefore of special phylogenetic interest. They constitute an important part of the biomass of the seashore, estuaries, fresh water and terrestrial soils. Moreover, they occupy a central position in the trophic networks, as a major food source for fishes, birds and terrestrial fauna. Among Annelids, the large majority of polychaetes is restricted to the marine domain. This report gives an overview of the immune strategies developed by polychaetes to fight pathogens. The potential and interest to use these worms as biomarkers to monitor the influence of environmental perturbation on the immunity of …
Neurogenesis In Cephalopods: "Eco-Evo-Devo" Approach In The Cuttlefish Sepia Officinalis (Mollusca-Cephalopoda), Sandra Navet, Sébastien Baratte, Yann Bassaglia, Aude Andouche, Auxane Buresi, Laure Bonnaud
Neurogenesis In Cephalopods: "Eco-Evo-Devo" Approach In The Cuttlefish Sepia Officinalis (Mollusca-Cephalopoda), Sandra Navet, Sébastien Baratte, Yann Bassaglia, Aude Andouche, Auxane Buresi, Laure Bonnaud
Journal of Marine Science and Technology–Taiwan
Cephalopods are new evolutionary and ecological models. By their phylogenetic position (Lophotrochozoa, Mollusca), they provide a missing master piece in the whole puzzle of neurodevelopment studies. Their derived and specific nervous system but also their convergence with vertebrates offer abundant materials to question the evolution and development of the nervous system of Metazoa (evo-devo studies). In addition, their various adaptions to different modes of life open new fields of investigation of developmental plasticity according to ecological context (eco-evo-devo approach). In this paper, we review the recent works on cephalopod nervous developmental investigations. We show how cephalopods, and especially Sepia officinalis, …
Traditional Vs New Approaches For Assessing Coral Health: A Global Overview And The Paradigm Of French Polynesia, Laetitia Hédouin, Véronique Berteaux-Lecellier
Traditional Vs New Approaches For Assessing Coral Health: A Global Overview And The Paradigm Of French Polynesia, Laetitia Hédouin, Véronique Berteaux-Lecellier
Journal of Marine Science and Technology–Taiwan
Today, there is increasing concern regarding the capacity of Scleractinian corals to sustain the growing number of insults associated with global and local changes (e.g. global warming, ocean acidification, pollution). If corals are to exist, there is an urgent need to use indicators of coral health that provide insight into early sub lethal shifts in corals before the irreversible effects of exposure manifest at the population and community levels. This paper will provide an overview of the most relevant and appropriate indicators of coral health currently used in traditional monitoring programs (traditional approach) or those that could be used in …
Assessing The Potential Bacterial Origin Of The Chemical Diversity In Calcareous Sponges, Elodie Quévrain, Mélanie Roué, Isabelle Domart-Coulon, Marie-Lise Bourguet-Kondracki
Assessing The Potential Bacterial Origin Of The Chemical Diversity In Calcareous Sponges, Elodie Quévrain, Mélanie Roué, Isabelle Domart-Coulon, Marie-Lise Bourguet-Kondracki
Journal of Marine Science and Technology–Taiwan
The chemodiversity and cultivable bacterial diversity of temperate calcareous sponges were investigated in a time series of collection of two sponges, Leuconia johnstoni (Baerida, Calcaronea) collected from the northeastern Atlantic Ocean and Clathrina clathrus (Clathrinida, Calcinea) collected from the northwestern Mediterranean Sea, using combined chemical and microbiological approaches. Bacteria were visualized in tissue sections of these sponges with Gram staining and in situ hybridization. The sponge crude extracts revealed annually persistent biological activities against reference human pathogen strains: L. johnstoni extracts displayed antimicrobial activity against a Gram positive Staphylococcus aureus strain; C. clathrus extracts displayed a broad spectrum of antimicrobial …
Diversity Of Bacterial Communities On Sunken Woods In The Mediterranean Sea, Sandrine Bessette, Sonja K. Fagervold, Chiara Romano, Daniel Martin, Nadine Le Bris, Pierre E Galand
Diversity Of Bacterial Communities On Sunken Woods In The Mediterranean Sea, Sandrine Bessette, Sonja K. Fagervold, Chiara Romano, Daniel Martin, Nadine Le Bris, Pierre E Galand
Journal of Marine Science and Technology–Taiwan
Sunken woods are very rich and diverse ecosystems supporting large macrofaunal diversity and representing a source of carbon and energy for any heterotrophic organism able to consume plant material, and those relying on specialized microbial taxa. However, relatively little is known about the microbial communities that degrade sunken woods and produce reduced compounds that serve as energy sources for chemosynthetic lifestyles. The purpose of this study was to explore the bacterial diversity developing on and within sunken woods in a NW Mediterranean submarine canyon and its adjacent slope by using 16S rRNA genes survey. We described communities from Pine wood …
Crustacean Hyperglycemic Hormone: Structural Variants, Physiological Function, And Cellular Mechanism Of Action, Chi-Ying Lee, Kuo-Wei Tsai, Wei-Shiun Tsai, Jia-Ying Jiang,, Yan-Jhou Chen
Crustacean Hyperglycemic Hormone: Structural Variants, Physiological Function, And Cellular Mechanism Of Action, Chi-Ying Lee, Kuo-Wei Tsai, Wei-Shiun Tsai, Jia-Ying Jiang,, Yan-Jhou Chen
Journal of Marine Science and Technology–Taiwan
Crustacean hyperglycemic hormone (CHH) is a peptide hormone originally identified in the X-organ/sinus gland (XO/ SG) complex of the eyestalks. It belongs to the CHH family which also includes molt-inhibiting hormone (MIH), vitellogenesis-inhibiting hormone (VIH), and mandibular organinhibiting hormone (MOIH), and ion transport peptide (ITP). Multiple molecular variants of CHH are generated by both post-transcriptional and post-translational mechanisms. In addition to the XO/SG complex, CHH gene is widely expressed in many extra-eyestalk tissues. Functionally, available data indicate that CHH is involved with the regulation of carbohydrate metabolism and stress-induced hyperglycemia. Several other physiological processes, including molting, ion and water balance, …
Hydrothermal Vent Effluents Affect Life Stages Of The Copepod Tisbe Sp, Hans-Uwe Dahms, Li-Chun Tseng, Derek Moo-Chul Shim, Jiang-Shiou Hwang
Hydrothermal Vent Effluents Affect Life Stages Of The Copepod Tisbe Sp, Hans-Uwe Dahms, Li-Chun Tseng, Derek Moo-Chul Shim, Jiang-Shiou Hwang
Journal of Marine Science and Technology–Taiwan
We examined the environmental effects of various concentrations of hydrothermal vent (HV) effluents on growth, reproduction, and survivorship of the Tisbe sp. harpacticoid copepod that were retrieved from localities near the vent. Developmental stages (nauplii and copepodids) were exposed to various concentrations of HV effluents in a static renewal culture system. In the survivorship experiments, we tested 3 distinct developmental phases in HV effluent dilutions from 50% to 1%. The HV effluents considerably reduced the survivorship of the naupliar stages at concentrations of >1% (P < 0.05); all nauplii died at concentrations of 25% and 50%. The copepodids were considerably affected at concentrations of >1% in Tisbe sp. (P < 0.05), and no copepodid survived at 50% (P < 0.01). The adult females died at a 50% concentration in Tisbe sp. The developmental duration was not considerably affected in the naupliar or copepodid phases; however, it exhibited a trend of developmental delay. The naupliar development of Tisbe sp. was substantially delayed at a concentration of 10% (P < 0.01), whereas copepodids and adults only exhibited a trend of delayed development with increasing HV concentration. The endpoint mortality exhibited a greater sensitivity to chemical exposure than the endpoint development time. The early developmental stages of Tisbe sp. in both traits were more sensitive to HV effluents than advanced stages. Mortality was a useful toxicological endpoint compared that of developmental duration. We demonstrated that Tisbe sp. may be used in the monitoring of acute and life cycle effects of natural marine pollution caused by HV effluents.
Prey Life History Variation Across A Predation Gradient, Matthew Baker, Dr. Mark Belk
Prey Life History Variation Across A Predation Gradient, Matthew Baker, Dr. Mark Belk
Journal of Undergraduate Research
Research exploring the effects of predation on life history evolution has focused almost exclusively on geographically disjunct populations subjected to discrete differences in mortality. Sampling only the ends of a predation continuum left to guess how prey life histories in the live bearing fish Brachyrhaphis rhabdopora vary by sampling at points along the entire predation gradient.
Ultrastructural Analysis Of Cartilage Matrix Fibrils, Niloufar Tabatabaei
Ultrastructural Analysis Of Cartilage Matrix Fibrils, Niloufar Tabatabaei
Journal of Undergraduate Research
Pulmonary Hypoplasia is a lethal neonatal disorder associated with the reduction of thoracic volume due to skeletal dysplasia.’ The homozygous recessive chondrodysplasia (cho/ cho) in mice serves as our model enabling us to demonstrate how the cartilage matrix is tied together on a molecular level. Atypically wide type II collagen fibrils have been observed in the cartilage of cho/ cho fetuses. This suggests that the mutation has altered the process by which collagen is assembled into fibrils in the extracellular matrix. Recently the cho mutation was mapped to chromosome 3 which is linked to a! (XI) collagen. Based upon this …
The Effects Of Prenatal Phytoestrogen Exposure On Fetal Brain Calbindin Levels, Heather Taylor, Dr. Edwin Lephart
The Effects Of Prenatal Phytoestrogen Exposure On Fetal Brain Calbindin Levels, Heather Taylor, Dr. Edwin Lephart
Journal of Undergraduate Research
Calcium plays a major part in muscle contraction, bone growth, but especially in central nervous system (CNS) development and function. Calcium participates in neuronal growth and degeneration. For example, the CNS depends on calcium during the development of neuronal cytoskeletal structures and synapse formation. When there is an excess of calcium, it disrupts the formation and the essential synapses or connections and they fail to form correctly.
Ultrasonic Study Of Normal Tendons And Ligaments Of The American Quarter Horse, Joseph Thurgood
Ultrasonic Study Of Normal Tendons And Ligaments Of The American Quarter Horse, Joseph Thurgood
Journal of Undergraduate Research
The palmar metacarpal aspect of 27 purebred (n=24) and appendix (n=3) American Quarter Horses were divided into sections and ultrasonically viewed to determine the circumference and cross-sectional area of the four principle tendons and ligaments in this region: superficial digital flexor tendon, deep digital flexor tendon, inferior check ligament, and suspensory ligament. This study was conducted using Brigham Young University Equitation schooling horses between May 1997 and February 1998. The structure measurements were analyzed for relationships to weight, age, sex and size of the horses (n=27). No correlation between weight, age, and sex of the horse and dimensions of any …
Phylogeny Of The Order Siphonaptera Based On Molecular Data, Alison Swindle, Dr. Michael F. Whiting
Phylogeny Of The Order Siphonaptera Based On Molecular Data, Alison Swindle, Dr. Michael F. Whiting
Journal of Undergraduate Research
The insect order Siphonaptera (fleas) has been historically significant for thousands of years. Beginning as early as 430 B.C. outbreaks of the bubonic plague have swept across continents and killed millions of people. Before fleas were linked with the plague, they were a little known order. Karl Linne recognized only two species of fleas in his “Systemae Naturae” in 1735. From 1735 to 1897 the described species count increased slowly from two up to sixty-eight. After 1897, and the recognition that fleas may be carriers of the plague, there was a rapid escalation in the research and interest in the …
Determining The Expression Patterns Of Developmental Genes In Tribolium, Cecily P. Vaughn, Dr. Randy Bennett
Determining The Expression Patterns Of Developmental Genes In Tribolium, Cecily P. Vaughn, Dr. Randy Bennett
Journal of Undergraduate Research
Development in animals is controlled by a set of genes called homeotic genes whose protein products regulate the expression of other genes. Although many homeotic genes have been locates in numerous organisms, there is still much that remains to be learned about the manner in which these genes control development. The aim of my ORCA project was to determine the expression patterns of a few key developmental genes in the red flour beetle (Tribolium castaneum).
A Global Analysis Of The Impacts Of Urbanization On Bird And Plant Diversity Reveals Key Anthropogenic Drivers, Myla F.J. Aronson, Frank A. La Sorte, Charles H. Nilon, Madhusudan Katti, Mark A. Goddard, Christopher A. Lepczyk, Paige S. Warren, Nicholas S.G. Williams, Sarel Cilliers, Bruce Clarkson, Cynnamon Dobbs, Rebecca W. Dolan, Marcus Hedblom, Stefan Klotz, Jip Louwe Kooijmans, Ingolf Kühn, Ian Macgregor-Fors, Mark Mcdonnell, Ulla Mörtberg, Petr Pyšek, Stefan Siebert, Jessica Sushinsky, Peter Werner, Marten Winter
A Global Analysis Of The Impacts Of Urbanization On Bird And Plant Diversity Reveals Key Anthropogenic Drivers, Myla F.J. Aronson, Frank A. La Sorte, Charles H. Nilon, Madhusudan Katti, Mark A. Goddard, Christopher A. Lepczyk, Paige S. Warren, Nicholas S.G. Williams, Sarel Cilliers, Bruce Clarkson, Cynnamon Dobbs, Rebecca W. Dolan, Marcus Hedblom, Stefan Klotz, Jip Louwe Kooijmans, Ingolf Kühn, Ian Macgregor-Fors, Mark Mcdonnell, Ulla Mörtberg, Petr Pyšek, Stefan Siebert, Jessica Sushinsky, Peter Werner, Marten Winter
Scholarship and Professional Work - LAS
Urbanization contributes to the loss of the world's biodiversity and the homogenization of its biota. However, comparative studies of urban biodiversity leading to robust generalities of the status and drivers of biodiversity in cities at the global scale are lacking. Here, we compiled the largest global dataset to date of two diverse taxa in cities: birds (54 cities) and plants (110 cities). We found that the majority of urban bird and plant species are native in the world's cities. Few plants and birds are cosmopolitan, the most common beingColumba livia and Poa annua. The density of bird and …
Chelex, Guanidine, And Phenol/Chloroform: A Comparison Of Extractions For Ancient Dna From Dentin Powder, J. Daniel Rasband
Chelex, Guanidine, And Phenol/Chloroform: A Comparison Of Extractions For Ancient Dna From Dentin Powder, J. Daniel Rasband
Journal of Undergraduate Research
Several different methods have been employed in the extraction and amplification of ancient DNA (aDNA). The work in this thesis Is an effort to compare three popular extraction techniques, chelex, guanidine/ silica slurry, and phenol/chloroform, as applied to extracting DNA from the dentin of ancient teeth. Modern and ancient samples were extracted using the three techniques. The extract was then amplified using the polymerase chain reaction (PCR). The data show that of the three extraction procedures, the most favorable results were obtained using the guanidine/silica slurry extraction technique, producing consistently good amplification. The phenol/chloroform extraction was mildly successful, showing amplification …
Determination Of The Cell Tvpe(S) In The Rat Adrenal Zona Glomerulosa That Release Interleukin-6, Michael F. Erickson
Determination Of The Cell Tvpe(S) In The Rat Adrenal Zona Glomerulosa That Release Interleukin-6, Michael F. Erickson
Journal of Undergraduate Research
Recent studies have shown that the cytokines interleukin-6 (IL-6) and tumor necrosis factor (TNF) are produced by cells found in the zonaglomerulosa of the rat adrenal cortex (I ,2). These cytokines modify adrenal hormone release gland. Specifically, IL-6 stimulates the release of glucocorticoids from the zona fasciculata/reticularis (3) and TNF inhibits the release of aldosterone from the zona glomerulosa (4). It is of interest that during times of traumatic injury or septic shock the serum and tissue levels of these cytokines increase. This increased cytokine level is partly responsible for the lethal effects of septic shock (including severe hypotension) since …
Culture And Isolation Of Embryonic Stem Cells From Porcine Blatocysts, J. Zachary Bruneel, Dr. Jeralyn J. Franson
Culture And Isolation Of Embryonic Stem Cells From Porcine Blatocysts, J. Zachary Bruneel, Dr. Jeralyn J. Franson
Journal of Undergraduate Research
Embryonic stem (ES) cells are obtained through the culture of undifferentiated cells of embryos in early devel-opment. Because embryonic cells will undergo differentiation and development when placed in standard culture conditions, a media which contains differentiation inhibitors must be used in order for successful development of ES cells. ES cells are capable of becoming any cell type in the body when removed from the influence of differentiation inhibitors, making ES cells a potential agent for gene transfer and livestock cloning. ES cells are therefore an invaluable research tool, providing both a better understanding of genetics as well as providing genetically …
The Impact Of A Naturally Fragmented Habitat On The Distribution And Breeding Biology Of House Wrens, Suzanne Reeve, Dr. Brian A. Maurer
The Impact Of A Naturally Fragmented Habitat On The Distribution And Breeding Biology Of House Wrens, Suzanne Reeve, Dr. Brian A. Maurer
Journal of Undergraduate Research
The distribution and life history characteristics of organisms can be significantly affected by structural variation in their habitats. One major type of variation is spatial arrangement and whether a habitat extends continuously or exists in small, fragmented patches. The effects of this variation have been documented in organisms from fruit flies (Jaenike 1978) to Amazonian beetles (Klein 1989) to songbirds (Andren and Angelstam 1988, Gates and Gysel 1978). According to research findings and theoretical prediction, many significant consequences of habitat fragmentation are caused by an increased ratio of habitat edge length to area. Studies indicate that increased edge length creates …
Ciliary Transport Of Particles In Polyplacophoran Gills, Matthew Palmer, Dr. Lee F. Braithwaite
Ciliary Transport Of Particles In Polyplacophoran Gills, Matthew Palmer, Dr. Lee F. Braithwaite
Journal of Undergraduate Research
Throughout the phylum Mollusca, gills are used to accomplish a variety of tasks and are thus highly specialized organs. The chitons, class Polyplacophora, are a primitive group of molluscs whose gills have the unique condition of being aligned on either side of the foot in series of up to 60 gills per side. Despite this unique arrangement, chiton gills have been the subject of relatively few studies. The objective of this project was to elucidate the mechanism by which chitons extrude particles from their gill surfaces. The two approaches employed to this end were, first, observation of live chitons transporting …
The Effects Of Maternal Separation On Lymphocyte Activity In Rats, Patrick R. Olson, Dr. Reuben Ward Rhees
The Effects Of Maternal Separation On Lymphocyte Activity In Rats, Patrick R. Olson, Dr. Reuben Ward Rhees
Journal of Undergraduate Research
We have been investigating how stress affects physiological development. My interest dealt with the effects of stress on the immune system. Lymphocyte proliferation is a common measurement of immune function.
A Conceptual Framework For Clutch-Size Evolution In Songbirds, Thomas E. Martin
A Conceptual Framework For Clutch-Size Evolution In Songbirds, Thomas E. Martin
Wildlife Biology Faculty Publications
Causes of evolved differences in clutch size among songbird species remain debated. I propose a new conceptual framework that integrates aspects of traditional life-history theory while including novel elements to explain evolution of clutch size among songbirds. I review evidence that selection by nest predation on length of time that offspring develop in the nest creates a gradient in offspring characteristics at nest leaving (fledging), including flight mobility, spatial dispersion, and self-feeding rate. I postulate that this gradient has consequences for offspring mortality rates and parental energy expenditure per offspring. These consequences then determine how reproductive effort is partitioned among …
Variable Facial Plumage In Juvenile Cliff Swallows: A Potential Offspring Recognition Cue?, Allison E. Johnson, Steven Freedberg
Variable Facial Plumage In Juvenile Cliff Swallows: A Potential Offspring Recognition Cue?, Allison E. Johnson, Steven Freedberg
School of Biological Sciences: Faculty Publications
Although the ability to recognize related offspring is essential in the evolution of social behavior, the cues that birds use to identify their own offspring are not fully understood. The Cliff Swallow (Petrochelidon pyrrhonota) is a highly social species that nests in large colonies and exhibits a high incidence of both conspecific brood parasitism and extrapair fertilization, behaviors that can potentially lead to parents investing energy and resources in unrelated offspring, which reduces the parents’ genetic fitness. Because parents continue to feed offspring after they leave the nest, parents also risk investing in unrelated offspring by misidentifying their …
Biophysical Mechanism Of Anesthetics, David Busath
Biophysical Mechanism Of Anesthetics, David Busath
Journal of Undergraduate Research
I was awarded a MEG in 2008 (with support continuing through 2009) for the project entitled “Biophysical Mechanism of Anesthetics.” The goals of the project were to measure the impact of volatile general anesthetics (isoflurane, sevoflurane, and desflurane) on:
Culturally Speaking: The Time Is Right For Drinkable Yogurts To Take Off In The United States, Phillip Tong
Culturally Speaking: The Time Is Right For Drinkable Yogurts To Take Off In The United States, Phillip Tong
Dairy Science
No abstract provided.
A Review Of Mastitis Control Practices, Rebecca Gooder
A Review Of Mastitis Control Practices, Rebecca Gooder
Dairy Science
No abstract provided.
Personality Affects Learning And Trade-Offs Between Private And Social Information In Guppies, Poecilia Reticulate, Larissa Trompf, Culum Brown
Personality Affects Learning And Trade-Offs Between Private And Social Information In Guppies, Poecilia Reticulate, Larissa Trompf, Culum Brown
Sentience Collection
The acquisition of information such as the location and quality of food, mates or shelter is a key survival requirement for animals. Individuals can acquire information through personal experience (private information) or through observing and interacting with others (social information). Environmental spatial and temporal heterogeneity can mean that sometimes social information conflicts with private knowledge. We tested how personality affected the importance placed on public versus private information in wild female guppies when these two information sources came into conflict. We found that boldness and sociality affected decisions to use conflicting social and private information. Bolder females used social information …
A Dichotomous Key For The Identification Of Nine Salmonids Of The Inland Northwest Using Six Diagnostic Skull Bones : And Associated Equations To Estimate Total Length And Weight From Bones Ingested By Piscivores Or Found In Archeological Sites, Aaron G. Stroud, Allan T. Scholz, Fisheries Research Center (Cheney, Wash.)
A Dichotomous Key For The Identification Of Nine Salmonids Of The Inland Northwest Using Six Diagnostic Skull Bones : And Associated Equations To Estimate Total Length And Weight From Bones Ingested By Piscivores Or Found In Archeological Sites, Aaron G. Stroud, Allan T. Scholz, Fisheries Research Center (Cheney, Wash.)
Biology Faculty Publications
No abstract provided.
Kentucky Warbler (Vol. 90, No. 1), Kentucky Library Research Collections
Kentucky Warbler (Vol. 90, No. 1), Kentucky Library Research Collections
The Kentucky Warbler
No abstract provided.
The Marine Heat Wave Off Western Australia During The Summer Of 2010/11 – 2 Years On, Nick Caputi, Gary Jackson, Alan Pearce
The Marine Heat Wave Off Western Australia During The Summer Of 2010/11 – 2 Years On, Nick Caputi, Gary Jackson, Alan Pearce
Fisheries Research Reports
This second workshop on the ‘Marine heat wave - 2 years on’ was held on 11 March 2013 about two years after the original event in the summer of 2010/11. The first workshop focused on the oceanographic conditions associated with the event as well as the short-term effects such as fish kill and southerly range extension of a number of tropical fish species. The second workshop concentrated on the oceanographic conditions since the workshop and the longer-term (2 years) effect on fisheries and the marine environment.
Fisheries Research Report No. 244 - Independent Observations Of Catches And Subsurface Mitigation Efficiencies Of Modified Trawl Nets For Endangered, Threatened And Protected Megafauna Bycatch In The Pilbara Fish Trawl Fishery, Corey B. Wakefield, Stuart Blight, Adrian W. Thomson, Clinton Syers, Shane O'Donoghue
Fisheries Research Report No. 244 - Independent Observations Of Catches And Subsurface Mitigation Efficiencies Of Modified Trawl Nets For Endangered, Threatened And Protected Megafauna Bycatch In The Pilbara Fish Trawl Fishery, Corey B. Wakefield, Stuart Blight, Adrian W. Thomson, Clinton Syers, Shane O'Donoghue
Fisheries Research Reports
Mitigation of endangered, threatened and protected (ETP) species is a challenge in many commercial fisheries globally and independent observer programs are often implemented to determine accurate estimates of interaction rates. However, interactions with ETP species may be extremely rare requiring very high and therefore costly levels of observer coverage to provide adequate statistical rigor for such programs.