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2009

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Articles 811 - 835 of 835

Full-Text Articles in Animal Sciences

Recent Occurrence Of An American Black Bear In Nebraska, Justin D. Hoffman, Sam Wilson, Hugh H. Genoways Jan 2009

Recent Occurrence Of An American Black Bear In Nebraska, Justin D. Hoffman, Sam Wilson, Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

We report on a young male, cinnamon-phase American black bear (Ursus americanus) that was collected in northwestern Nebraska on May 12, 2008. This specimen represents the first black bear taken in Nebraska since 1907. Cranial characteristics and mass of the individual indicated a 15–16-month old bear. This bear may have dispersed from the Laramie Mountains in southeastern Wyoming along the North Platte River into western Nebraska. This record adds to the growing number of cases where large carnivores are reinvading previously occupied territory in the Great Plains. These reinvasions potentially have broad ecological implications to local prey populations …


Great Curassow And Crested Guan Near Ciudad Victoria, Tamaulipas, Mexico, Timothy Brush Jan 2009

Great Curassow And Crested Guan Near Ciudad Victoria, Tamaulipas, Mexico, Timothy Brush

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Great Curassow (Crax rubra) and Crested Guan (Penelope purpurascens) were rediscovered in the Ciudad Victoria area of southwestern Tamaulipas, Mexico for the first time since 1909. Birds were found in low elevation canyons containing permanent running water, with riparian forest dominated by Montezuma bald-cypress (Taxodium mucronatum) and steep scrub-covered slopes. These records represent the northernmost known points of the ranges of both species. Additional field work will be needed to determine whether these small populations are isolated from the nearest known populations in the El Cielo/Gómez Farías area of southern Tamaulipas.


Identification And Characterization Of The Glucagon-Like Peptide-2 Hormonal System In Ruminants, Christina C. Taylor Edwards Jan 2009

Identification And Characterization Of The Glucagon-Like Peptide-2 Hormonal System In Ruminants, Christina C. Taylor Edwards

University of Kentucky Doctoral Dissertations

The hormone glucagon-like peptide-2 (GLP-2) is important in the regulation of intestinal growth and blood flow in nonruminant animals. However, no research reports the existence of GLP-2 in ruminants. Therefore, this dissertation examined the existence of GLP-2 and its receptor, their response to physiological stimuli, and its ability to induce gastrointestinal growth and intestinal blood flow in ruminants.

Experiments 1 and 2 established the gastrointestinal distribution of mRNA for proglucagon (the GLP-2 precursor) and the GLP-2 receptor. Furthermore, these experiments determined the effects of changing dietary energy intake on plasma GLP-2 concentrations and proglucagon and GLP-2 receptor mRNA expression. Experiment …


Evaluation Of Dietary Alterations That Have Potential To Affect Feed Intake And Feed Preference In Swine, James Seth Monegue Jan 2009

Evaluation Of Dietary Alterations That Have Potential To Affect Feed Intake And Feed Preference In Swine, James Seth Monegue

University of Kentucky Master's Theses

Feed intake is a key factor affecting pig performance; thus, the objective of these studies was to assess a variety of factors that could potentially affect intake in pigs in different production stages. Studies were conducted to determine the effects of flavor and diet complexity, Appetein™ (an alternative protein source), and graded levels of salt on swine feed intake and feed preference. Two newly developed flavors were used in nursery pig diets. The use of the two flavors did not increase feed intake (P > 0.05). Nursery pigs actually showed a preference for the control diet. Complex diet formulation does increase …


Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n Jan 2009

Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to identify the presence and prevalence of bovine tuberculosis in the eastern Anatolian border province of Van and its villages and to collect the preliminary data required for programs for the prevention and control of this zoonotic disease. The tuberculin test was performed on 210 cattle. Nasal swab and milk samples were obtained from the animals tested. Three cattle that had a positive tuberculin test also tested positive for the DNA target, a 580-bp fragment of IS6110 specific for members of the Mycobacterium tuberculosis complex. This fragment was also recovered from milk taken from …


Renal Handling Of Ciprofloxacin In Domestic Ruminant Species, Ijaz Javed, Zahid Iqbal, Zia-Ur Rahman, Muhammad Zargham Khan, Faqir Muhammad, Faqir H. Khan, Javed I. Sultan Jan 2009

Renal Handling Of Ciprofloxacin In Domestic Ruminant Species, Ijaz Javed, Zahid Iqbal, Zia-Ur Rahman, Muhammad Zargham Khan, Faqir Muhammad, Faqir H. Khan, Javed I. Sultan

Turkish Journal of Veterinary & Animal Sciences

Investigations regarding renal clearance and urinary excretion of ciprofloxacin were carried out in buffaloes, cows, sheep, and goats. The drug was administered intramuscularly at a dose of 5 mg/kg body weight. The plasma and urine concentrations of the drug were determined with HPLC. Renal clearance of endogenous creatinine was used as an index of glomerular filtration rate (GFR). In buffaloes, it was 0.72 ± 0.15 (mean ± SE), in cows 0.36 ± 0.09, in sheep 1.33 ± 0.35, and in goats 1.87 ± 0.38 mL/min/kg. The renal clearance of ciprofloxacin in buffaloes was 0.47 ± 0.08, in cows 0.29 ± …


Isolation And Biotyping Of Brucella Melitensis From Aborted Sheep And Goat Fetuses, Esra Büyükcangaz, Ayşi̇n Şen, Serpi̇l Kahya Jan 2009

Isolation And Biotyping Of Brucella Melitensis From Aborted Sheep And Goat Fetuses, Esra Büyükcangaz, Ayşi̇n Şen, Serpi̇l Kahya

Turkish Journal of Veterinary & Animal Sciences

The possible role of Brucella spp. in 65 abortion cases, 55 from sheep and 10 from goats, occurring in the birth seasons of 2004 and 2005 in northwestern Turkey was investigated. Colony morphology, agglutination by acriflavin, H2S production, CO2 requirement, dye sensitivity in thionin, basic fuchsin, growth characteristics in streptomycin, lysis with Tbilisi phage, and agglutination with monospecific A- and M antisera were examined for identification and biotyping. The isolates from 21 of 55 sheep and 1 of 10 goat abortion cases were identified as Brucella melitensis. Biotyping revealed that 14, 6, and 1 of 22 Brucella spp. were B. …


Effect Of Phosalone On Haematological Indices In The Tilapia, Oreochromis Mossambicus, H. Abdul Jaffar Ali, V. Jaya Rani Jan 2009

Effect Of Phosalone On Haematological Indices In The Tilapia, Oreochromis Mossambicus, H. Abdul Jaffar Ali, V. Jaya Rani

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to assess the effect of phosalone on the tilapia, Oreochromis mossambicus. The effect was assessed on the basis of the results of acute toxicity tests and comparison of results of haematological examination of a control and an experimental group exposed to phosalone. The 96 h LC_{50} is the basic value in the acute toxicity test and it was 0.26 mg/L for phosalone against the test fish. Haematological profile of the healthy fish was carried out with 2 sublethal concentrations, viz 0.0325 mg/L (1/8) and 0.065 mg/L (1/4) of phosalone. The experiment was performed semi-statically …


Structural Aberration In Ha Associated With Adaptation Of Human Influenza A H3n2 Virus In Embryonated Chicken Eggs, Viroj Wiwanitkit Jan 2009

Structural Aberration In Ha Associated With Adaptation Of Human Influenza A H3n2 Virus In Embryonated Chicken Eggs, Viroj Wiwanitkit

Turkish Journal of Veterinary & Animal Sciences

Molecular changes associated with adaptation of human influenza A virus in embryonated chicken eggs are reported: 4 amino acid substitutions occurred in HA (G186V, S219F, V226I, V309I). These substitutions allowed binding to SAalpha2,3Gal- and SAalpha2,6Gal-containing receptors, conferred SAalpha2,3Gal specificity, and preserved antigenicity. Here, the author reports the result of protein secondary structure predictions of the adaption from its primary sequence using the NNPREDICT server. Comparing the classical HA of H3N2, the adaptation has additional helices and strands and these identified structural changes should be the explanation for the reported binding and antigenicity changes.


Physiological And Biochemical Responses In German Fawn Kids Subjected To Cooling Treatments Under Mediterranean Climate Conditions, Sezen Ocak, Nazan Darcan, Soner Çankaya, Tamer C. İnal Jan 2009

Physiological And Biochemical Responses In German Fawn Kids Subjected To Cooling Treatments Under Mediterranean Climate Conditions, Sezen Ocak, Nazan Darcan, Soner Çankaya, Tamer C. İnal

Turkish Journal of Veterinary & Animal Sciences

This study was conducted to determine the effects of different physical modifications (sprinkler and fan) related to heat stress during summer on relevant blood biochemical measures and physiological responses, such as rectal temperature (RT), respiration rate (RR), heart rate (HR), testis temperature (TT), and head temperature (HT), in kids. In total, 32 male German Fawn × Hair kids at 6 months of age were divided into 4 groups of 8. Treatments were as follows: group A: (no sprinkler, no fan) (control); group B: sprinkler only (at 1000-1100 and 1400-1500), no fan; group C: fan (1.5 m/h between 1000-1800), no sprinkler; …


Efficacy Of 2-Phenoxyethanol As An Anaesthetic For The Musky Octopus, Eledone Moschata (Lamarck 1799), (Cephalopoda: Octopodidae), Hali̇l Şen, Tansel T. Tanrikul Jan 2009

Efficacy Of 2-Phenoxyethanol As An Anaesthetic For The Musky Octopus, Eledone Moschata (Lamarck 1799), (Cephalopoda: Octopodidae), Hali̇l Şen, Tansel T. Tanrikul

Turkish Journal of Veterinary & Animal Sciences

The efficacy of 2-phenoxyethanol (2-PhOH) as an anaesthetic for the musky octopus, Eledone moschata (Lamarck, 1799), was examined. Different doses (1.0, 1.2, 1.4, 1.6, and 1.8 mL L^{-1}) of 2-PhOH were prepared in 20 L transparent plastic containers containing 10 L of continually aerated seawater (pH 8.2, O2 8 mg L^{-1}, salinity 37.2 per thousand at 15.3 °C). Six individuals were used in each experiment. After quantifying the anaesthetic time for each animal, which was defined as the loss of sucking intensity (Stage A3), the specimens were transferred to a plastic recovery tank (430 L of aerated seawater), defined as …


A Case Report Of Ulcerative Lymphangitis (A Mini Review Of Causes And Current Therapies), Fereydon Rezazadeh Zavoshti, Amir Babak Sioofy-Khojine, Hassan Ali Mahpeikar Jan 2009

A Case Report Of Ulcerative Lymphangitis (A Mini Review Of Causes And Current Therapies), Fereydon Rezazadeh Zavoshti, Amir Babak Sioofy-Khojine, Hassan Ali Mahpeikar

Turkish Journal of Veterinary & Animal Sciences

Ulcerative lymphangitis, or pigeon fever, is a bacterial infection of the lymphatic vessels of the skin in horses, cattle, sheep, and goats. Corynebacterium pseudotuberculosis is the classical cause of the disease. However, other pathogens such as Staphylococcus aureus have been isolated from cases of ulcerative lymphangitis. The aim of this report is to review the causes of pyoderma and skin abscesses in horses with specific attention to Staphylococcus aureus. A 10-year-old Thoroughbred stallion was examined in the suburbs of Tabriz (northwest of Iran) in February 2007. Skin abscesses were present around the hock joints on the hind limbs, on the …


The Effect Of Seasonality On The Health And Growth Of A Newly Recorded Myxobolus Species Infecting Cultured Sharp Snout Seabream (Diplodus Puntazzo C.), Eleni Golomazou, Fotini Athanassopoulou, Eudokia Karagouni, Lambros Kokkokiris Jan 2009

The Effect Of Seasonality On The Health And Growth Of A Newly Recorded Myxobolus Species Infecting Cultured Sharp Snout Seabream (Diplodus Puntazzo C.), Eleni Golomazou, Fotini Athanassopoulou, Eudokia Karagouni, Lambros Kokkokiris

Turkish Journal of Veterinary & Animal Sciences

The prevalence, intensity, and pathology of a Myxobolus sp. affecting the kidneys of cultured Diplodus puntazzo C. during the period 2003-2005 are described. The study included data collected from the largest farm in southern Greece where a high rate of mortality due to myxosporean infection occurred. Sharp snout seabream reared in sea cages in southern Greece underwent microbiological, parasitological, and histological examinations, while measurement and identification of parasites was performed immediately after sampling. Samplings were made daily from winter 2003 to autumn 2005. Prevalence was estimated for each season (autumn, winter, spring, and summer) each year (2003, 2004, and 2005). …


Investigation Of Mohair Production, Clean Yield, And Fibre Characteristics In Coloured Mohair Goat And F_1 Cross-Bred Kids Of Angora Goat X Coloured Mohair Goat, Fuat Odabaşioğlu, Mürsel Küçük, Orhan Yilmaz Jan 2009

Investigation Of Mohair Production, Clean Yield, And Fibre Characteristics In Coloured Mohair Goat And F_1 Cross-Bred Kids Of Angora Goat X Coloured Mohair Goat, Fuat Odabaşioğlu, Mürsel Küçük, Orhan Yilmaz

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to compare some fleece and fibre characteristics of kids of Coloured mohair goat with F_1 cross-bred kids of Angora x Coloured mohair goat to reveal the effects of this cross-breeding on mohair production, clean yield, and fibre quality. This study was carried out using goats of the Coloured mohair goat breed at the Experimental Farm of the Faculty of Veterinary Medicine of Yüzüncü Yıl University. Four Angora goats were used as male material. In 2001, greasy fleece weight, clean mohair yield, fibre diameter, fibre length, fibre elasticity, breaking strength, kemp fibre content, and medullated …


Comparison Of The Results Of Serum Total Protein Concentration Measured By 3 Methods: Preliminary Results, Teodora M. Georgieva, Dimitrinka S. Zapryanova, Evgeniya V. Dishlyanova, Stoyan I. Tanev, Ivan P. Georgiev, Maria I. Andonova, Ivan N. Kanelov, Lazarin V. Lazarov, Petya I. Koleva Jan 2009

Comparison Of The Results Of Serum Total Protein Concentration Measured By 3 Methods: Preliminary Results, Teodora M. Georgieva, Dimitrinka S. Zapryanova, Evgeniya V. Dishlyanova, Stoyan I. Tanev, Ivan P. Georgiev, Maria I. Andonova, Ivan N. Kanelov, Lazarin V. Lazarov, Petya I. Koleva

Turkish Journal of Veterinary & Animal Sciences

The present study provides the results from a comparative study of the 3 commonly used methods for total protein (TP) measurement. The experiments were carried out with 6 dogs (4-7 year-old, weighing 12.8 ± 1.4 kg). Five blood samples were obtained by saphena venepuncture from all dogs, during the time course of the experimentally induced infection with Staphylococcus intermedius, administered subcutaneously at a dose rate of 5 ml of 1.10^9 CFU/ml within 14 days. TP concentration was measured by 2 macro protein techniques - biuret method (commonly used) and method of Lowry, and a modified version of biuret method (micro …


The Ostrich (Struthio Camelus) Egg – A Safety Seat In The Time Vehicle, Ross G. Cooper, Marek Lukaszewicz, Jaroslaw O. Horbanczuk Jan 2009

The Ostrich (Struthio Camelus) Egg – A Safety Seat In The Time Vehicle, Ross G. Cooper, Marek Lukaszewicz, Jaroslaw O. Horbanczuk

Turkish Journal of Veterinary & Animal Sciences

The role of the egg is 2-fold - protection of the contents and conditioning the incubation. We used a variety of search engines to collate literature pertinent to the ostrich egg from PubMed, Medline, and Agricola. An ostrich egg weighs ca. 1.5 kg, and the mean length and width are 156 and 129 mm, respectively. The vertical and horizontal circumferences reach a maximum of 45 and 40 cm, respectively. Average internal volume is ca. 1350 ml. The mean weight of albumen in a 1500 g ostrich egg reaches 900 g, with a 317 g yolk and a 296 g voided …


Submucosal Uroliths In The Vesica Urinaria Of A Male Dalmatian Dog, İrem Gül Sancak, Azi̇z Arda Sancak, Fatma Eser Özgenci̇l Jan 2009

Submucosal Uroliths In The Vesica Urinaria Of A Male Dalmatian Dog, İrem Gül Sancak, Azi̇z Arda Sancak, Fatma Eser Özgenci̇l

Turkish Journal of Veterinary & Animal Sciences

A 1-year-old male Dalmatian dog with urinary obstruction was examined on an emergency basis. The macroscopic urine examination, direct radiography, positive contrast cystography, and ultrasonography revealed urolithiasis. Histology of the vesica urinaria showed urolith particles deeply embedded in a membrane on the mucosa. This is not commonly seen in dogs with urolithiasis.


Industrial Copper Intoxication Of Iranian Fat-Tailed Sheep In Kerman Province, Iran, Ali Asghar Mozaffari, Amin Derakhshanfar, Jamileh Salar Amoli Jan 2009

Industrial Copper Intoxication Of Iranian Fat-Tailed Sheep In Kerman Province, Iran, Ali Asghar Mozaffari, Amin Derakhshanfar, Jamileh Salar Amoli

Turkish Journal of Veterinary & Animal Sciences

An outbreak of industrial chronic copper poisoning in Iranian fat-tailed sheep in a region of southeast Iran is described. At least 10,000 out of 75,000 sheep (13.3%) originating from 50 flocks died over a period of 3 years in the Khatoon Abad region in Kerman province, Iran. The diagnosis was based on epidemiological, clinical, hematological, serum biochemical, urinalysis, necropsy and histopathological evaluations. Then findings were confirmed by toxicological analysis of liver and kidney samples. The details of epidemiological, clinical, laboratory and pathologic findings are described in the manuscript. These findings were indicative of chronic copper poisoning in affected sheep. Toxicological …


Immunohistochemical Studies On S100 Reactivity In Chicks Experimentally Infected With Eimeria Tenella I: The Localization Of S100 Protein And Its Subunits \Alpha And \Beta In Stages Of Eimeria Tenella, Lati̇fe Beyaz, Ayhan Atasever, Feyzullah Beyaz, Anil İça Jan 2009

Immunohistochemical Studies On S100 Reactivity In Chicks Experimentally Infected With Eimeria Tenella I: The Localization Of S100 Protein And Its Subunits \Alpha And \Beta In Stages Of Eimeria Tenella, Lati̇fe Beyaz, Ayhan Atasever, Feyzullah Beyaz, Anil İça

Turkish Journal of Veterinary & Animal Sciences

The expression of the calcium-binding protein S100 and its subunits \alpha and \beta in the intracellular protozoon Eimeria tenella was investigated. For this purpose, anti-S100 whole molecule polyclonal antibody and anti-S100 \alpha and anti-S100 \beta monoclonal antibodies were used on paraffin sections collected from the cecum, ileum, colon, rectum, and bursa of Fabricius of chicks experimentally infected with E. tenella. One hundred seventy-five 2- to 3-day-old specific pathogen-free chicks were used. The chicks were divided into experiment and control groups, to include 94 and 81 chicks, respectively. All the chicks in the experimental group received 10,000 spored oocytes in 0.5 …


Effect Of Leptin Gene Polymorphisms On Milk Production Traits Of Jersey Cows, Hanna Kulig, Marek Kmiec, Inga Kowalewska Luczak, Gabriela Andziak Jan 2009

Effect Of Leptin Gene Polymorphisms On Milk Production Traits Of Jersey Cows, Hanna Kulig, Marek Kmiec, Inga Kowalewska Luczak, Gabriela Andziak

Turkish Journal of Veterinary & Animal Sciences

A total of 181 Jersey cows were used to investigate how leptin gene polymorphisms affect milk production traits such as milk yield, fat and protein yield, and fat and protein content. Two single-nucleotide polymorphisms (SNPs) were genotyped, including the A59V in exon 3 and Sau3AI in intron 2. The genotype, allele, and haplotype frequencies were estimated. Statistical analysis revealed that the A59V polymorphism significantly affected the milk, protein, and fat yield (P Keywords: Leptin, SNP, Jersey cows, milk production traits


Esophageal Diverticulum In A Cat, Mehmet Kazim Börkü, Kerem Ural, Murat Ki̇bar, Yunusemre Özkanlar, Murat Güzel, Cenker Çağri Cingi Jan 2009

Esophageal Diverticulum In A Cat, Mehmet Kazim Börkü, Kerem Ural, Murat Ki̇bar, Yunusemre Özkanlar, Murat Güzel, Cenker Çağri Cingi

Turkish Journal of Veterinary & Animal Sciences

A 7-year-old spayed female domestic shorthair cat was presented because of post-prandial regurgitation, gasping shortly after eating, weight loss, dyspnea, and tachypnea. Six months prior to the onset of these signs the cat had received nutritional support via gastrostomy and then nasogastric tubes. A diagnosis of esophageal diverticulum was made on the basis of clinical features, contrast esophagram, and endoscopy, and was considered a complication of tube feeding. Medical therapy, including parenteral fluids, ranitidine, metoclopramide, and cisapride, and a soft, bland low-fat diet resulted in improvement in the clinical signs within 1 week. The cat was discharged and recovery was …


Dairy Farming In Kars District, Turkey: Iii. Neonatal Calf Health, Hi̇dayet Meti̇n Erdoğan, Ahmet Ünver, Mehmet Çi̇ti̇l, Vehbi̇ Güneş, Mükremi̇n Özkan Arslan, Mehmet Tuzcu, Hali̇l İbrahi̇m Gökce Jan 2009

Dairy Farming In Kars District, Turkey: Iii. Neonatal Calf Health, Hi̇dayet Meti̇n Erdoğan, Ahmet Ünver, Mehmet Çi̇ti̇l, Vehbi̇ Güneş, Mükremi̇n Özkan Arslan, Mehmet Tuzcu, Hali̇l İbrahi̇m Gökce

Turkish Journal of Veterinary & Animal Sciences

The study was designed to determine the health status of neonatal calves on dairy farms in Kars district. It involved retrospective questioning (2001 calving season) and prospective follow up (2002 calving season). The localities and farms were selected from a list using a 2-stage stratified random sampling method. Farms were weekly visited by authors during the 2002 calving season for clinical problems. A total of 624 calves, born on 45 farms from 7 different localities, were followed up in the 2002 calving season. On the same farms data were collected regarding 582 neonatal calves in the 2001 calving season by …


The Effect Of Zinc On L-Leucine Transport In Lobster Intestine, Anna Lynn Mullins Jan 2009

The Effect Of Zinc On L-Leucine Transport In Lobster Intestine, Anna Lynn Mullins

UNF Graduate Theses and Dissertations

The current study is a characterization of L-leucine absorption across the American lobster intestine in order to determine the likelihood of a bis-complex formation with zinc ([Leu]-Zn-[Leu]) as a possible substrate for PEPT1. This study required isolated lobster intestines to be mounted in a perfusion chamber in order to quantify 3H-L-leucine transport. It was hypothesized that an apical dipeptide transporter, PEPT1, was responsible for uptake of L-leucine via formation of a bis-complex with zinc ([Leu]-Zn-[Leu]) in the form of molecular mimicry, where the bis-complex mimics the normal dipeptide substrate of the proposed carrier system. It was found that L-leucine transport …


Showing Utah 4-H Dressage, Rebecca Lewis, Patricia Evans Jan 2009

Showing Utah 4-H Dressage, Rebecca Lewis, Patricia Evans

All Current Publications

This publication gives information on Dressage which teaches understanding between horse and rider. Dressage develops the horse's physical and mental condition.


The Guinea Pig Ileum Lacks The Direct, High-Potency, M2-Muscarinic, Contractile Mechanism Characteristic Of The Mouse Ileum, Michael T. Griffin, Minoru Matsui, Rennolds S. Ostrom, Frederick J. Ehlert Jan 2009

The Guinea Pig Ileum Lacks The Direct, High-Potency, M2-Muscarinic, Contractile Mechanism Characteristic Of The Mouse Ileum, Michael T. Griffin, Minoru Matsui, Rennolds S. Ostrom, Frederick J. Ehlert

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

We explored whether the M2 muscarinic receptor in the guinea pig ileum elicits a highly potent, direct-contractile response, like that from the M3 muscarinic receptor knockout mouse. First, we characterized the irreversible receptor-blocking activity of 4-DAMP mustard in ileum from muscarinic receptor knockout mice to verify its M3 selectivity. Then, we used 4-DAMP mustard to inactivate M3 responses in the guinea pig ileum to attempt to reveal direct, M2 receptor-mediated contractions. The muscarinic agonist, oxotremorine-M, elicited potent contractions in ileum from wild-type, M2 receptor knockout, and M3 receptor knockout mice characterized by negative log EC50 (pEC 50 ) values ± …