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2013

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Full-Text Articles in Animal Sciences

Spatial Analysis Of Factors Influencing Long-Term Stress In The Grizzly Bear (Ursus Arctos) Population Of Alberta, Canada, Mathieu Bourbonnais, Trisalyn Nelson, Mark Cattet, Chris T. Darimont, Gordon Stenhouse Dec 2013

Spatial Analysis Of Factors Influencing Long-Term Stress In The Grizzly Bear (Ursus Arctos) Population Of Alberta, Canada, Mathieu Bourbonnais, Trisalyn Nelson, Mark Cattet, Chris T. Darimont, Gordon Stenhouse

Physiology Collection

Non-invasive measures for assessing long-term stress in free ranging mammals are an increasingly important approach for understanding physiological responses to landscape conditions. Using a spatially and temporally expansive dataset of hair cortisol concentrations (HCC) generated from a threatened grizzly bear (Ursus arctos) population in Alberta, Canada, we quantified how variables representing habitat conditions and anthropogenic disturbance impact long-term stress in grizzly bears. We characterized spatial variability in male and female HCC point data using kernel density estimation and quantified variable influence on spatial patterns of male and female HCC stress surfaces using random forests. Separate models were developed for regions …


Beef Cattle Body Temperature During Climatic Stress: A Genome-Wide Association Study, Jeremy T. Howard, Stephen D. Kachman, Warren M. Snelling, E. John Pollak, Daniel C. Ciobanu, Larry A. Kuehn, Matthew L. Spangler Dec 2013

Beef Cattle Body Temperature During Climatic Stress: A Genome-Wide Association Study, Jeremy T. Howard, Stephen D. Kachman, Warren M. Snelling, E. John Pollak, Daniel C. Ciobanu, Larry A. Kuehn, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Cattle are reared in diverse environments and collecting phenotypic body temperature (BT) measurements to characterize BT variation across diverse environments is difficult and expensive. To better understand the genetic basis of BT regulation, a genome-wide association study was conducted utilizing crossbred steers and heifers totaling 239 animals of unknown pedigree and breed fraction. During predicted extreme heat and cold stress events, hourly tympanic and vaginal BT devices were placed in steers and heifers, respect i v e l y. I n d i v i dua l s we r e genotyped with t h e BovineSNP50K_v2 assay and data …


Dog Behavior Co-Varies With Height, Bodyweight And Skull Shape, Paul D. Mcgreevy, Dana Georgevsky, Johanna Carrasco, Michael Valenzuela, Deborah L. Duffy, James A. Serpell Dec 2013

Dog Behavior Co-Varies With Height, Bodyweight And Skull Shape, Paul D. Mcgreevy, Dana Georgevsky, Johanna Carrasco, Michael Valenzuela, Deborah L. Duffy, James A. Serpell

Eidonomy Collection

Dogs offer unique opportunities to study correlations between morphology and behavior because skull shapes and body shape are so diverse among breeds. Several studies have shown relationships between canine cephalic index (CI: the ratio of skull width to skull length) and neural architecture. Data on the CI of adult, show-quality dogs (six males and six females) were sourced in Australia along with existing data on the breeds’ height, bodyweight and related to data on 36 behavioral traits of companion dogs (n = 8,301) of various common breeds (n = 49) collected internationally using the Canine Behavioral Assessment and Research Questionnaire …


Evaluation Of Striped Bass Stocks In Virginia, Monitoring And Tagging Studies, 2010-2014 Progress Report, 1 September 2012 - 31 August 2013, Philip W. Sadler, Matthew W. Smith, John M. Hoenig, Shelley E. Sullivan, Robert E. Harris, Lydia M. Goins Dec 2013

Evaluation Of Striped Bass Stocks In Virginia, Monitoring And Tagging Studies, 2010-2014 Progress Report, 1 September 2012 - 31 August 2013, Philip W. Sadler, Matthew W. Smith, John M. Hoenig, Shelley E. Sullivan, Robert E. Harris, Lydia M. Goins

Reports

This report presents the results of striped bass (Morone saxatilis) tagging and monitoring activities in Virginia during the period 1 September 2012 through 31 August 2013. It includes an assessment of the biological characteristics of striped bass taken from the 2013 spring spawning run, estimates of annual survival and fishing mortality based on annual spring tagging, and the results of the study that documents the prevalence of mycobacterial infections of striped bass in Chesapeake Bay. The information contained in this report is required by the Atlantic States Marine Fisheries Commission and is used to implement a coordinated management plan for …


Observing The Unwatchable Through Acceleration Logging Of Animal Behavior, Danielle D. Brown, Roland Kays, Martin Wikelski, Rory Wilson, A. Peter Klimley Dec 2013

Observing The Unwatchable Through Acceleration Logging Of Animal Behavior, Danielle D. Brown, Roland Kays, Martin Wikelski, Rory Wilson, A. Peter Klimley

Methodology and Animal Models in Research

Behavior is an important mechanism of evolution and it is paid for through energy expenditure. Nevertheless, field biologists can rarely observe animals for more than a fraction of their daily activities and attempts to quantify behavior for modeling ecological processes often exclude cryptic yet important behavioral events. Over the past few years, an explosion of research on remote monitoring of animal behavior using acceleration sensors has smashed the decades-old limits of observational studies. Animal-attached accelerometers measure the change in velocity of the body over time and can quantify fine-scale movements and body postures unlimited by visibility, observer bias, or the …


The Cost Of Affordable Food, Candice L. Cobuzzi Dec 2013

The Cost Of Affordable Food, Candice L. Cobuzzi

SURGE

When someone puts a piece of food in front of me, I don’t just see a piece of food.

Instead, I see an innocent cow being cornered by a forklift and slaughtered, its limp, moist tissue hung on a long conveyer belt with hundreds of others.

I see hundreds of chemically-injected chickens packed into a dark barn with no hope of seeing sunlight in their lifetime.

I see immigrants pulled from their houses like criminals, taken away from the lives they’ve spent years building for themselves and their families, working for the same food company that courted them into the …


Transcriptional Regulation Of The Porcine Gnrh Receptor Gene By Glucocorticoids, Chanho Lee Dec 2013

Transcriptional Regulation Of The Porcine Gnrh Receptor Gene By Glucocorticoids, Chanho Lee

Department of Animal Science: Dissertations, Theses, and Student Research

Binding of GnRH to its receptor (GnRHR) stimulates the synthesis and secretion of the gonadotropins, as well as up-regulation of GnRHR. Thus, the interaction between GnRH and GnRHR represents a central point for regulation of reproduction. Glucocorticoids alter reproduction by reducing GnRH responsiveness of gonadotropes within the anterior pituitary gland, potentially via transcriptional regulation of the GnRHR gene. Investigation into this mechanism, however, revealed that the murine GnRHR gene was stimulated by glucocorticoids. To determine the effect of glucocorticoids on porcine GnRHR gene expression, gonadotrope-derived αT3-1 cells were transiently transfected with a vector containing 5118 bp of 5’ flanking sequence …


A Population Model For Walleye In Nebraska Irrigation Reservoirs, Robert A. Kill Dec 2013

A Population Model For Walleye In Nebraska Irrigation Reservoirs, Robert A. Kill

School of Natural Resources: Dissertations, Theses, and Student Research

Understanding how and why fish population size changes between years is a central theme in fisheries ecology. Fishery agencies have limited time and financial resources, thus there is a need for a quantitative way to direct the limited time and financial resources so agencies can manage fisheries more efficiently. I developed a tool for fishery managers that synthesizes common population indices and evaluated the relative importance of those indices given varying uncertainty in age-0 walleye Sander vitreus survival. Under most circumstances, I determined that resources are best utilized in reducing age-0 survival uncertainty when understanding walleye population growth. I applied …


Determining Scaphirhynchus Sturgeon Population Demographics And Dynamics: Implications For Range-Wide Management, Recovery, And Conservation, Martin J. Hamel Dec 2013

Determining Scaphirhynchus Sturgeon Population Demographics And Dynamics: Implications For Range-Wide Management, Recovery, And Conservation, Martin J. Hamel

School of Natural Resources: Dissertations, Theses, and Student Research

Sturgeons (Acipenseridae) have experienced world-wide declines as a result of anthropogenic effects such as over-harvest, habitat degradation, altered flow regimes, and pollution. Nearly all European and Asian sturgeon species have experienced population declines and have subsequently been classified as either threatened or endangered. North American sturgeons have experienced a similar plight in that all eight native sturgeon species are listed as endangered, threatened, or of special concern. Direct linkages between North American sturgeon declines and anthropogenic effects are difficult to assess due to scale considerations, fluctuating environmental conditions, difficulty in capture, and the interaction of all these effects. To recover, …


Culturally Speaking: You Don't Know Beans About Cottage Cheese, Phillip Tong Dec 2013

Culturally Speaking: You Don't Know Beans About Cottage Cheese, Phillip Tong

Dairy Science

No abstract provided.


Loss Of Vascular Endothelial Growth Factor A (Vegfa) Isoforms In The Testes Of Male Mice Causes Subfertility, Reduces Sperm Numbers, And Alters Expression Of Genes That Regulate Undifferentiated Spermatogonia, Ningxia Lu, Kevin M. De Avila, Debra T. Clopton, William E. Pohlmeier, Vanessa M. Brauer, Renee M. Mcfee, John S. Weber, Napoleone Ferrara, David W. Silversides, Andrea S. Cupp Dec 2013

Loss Of Vascular Endothelial Growth Factor A (Vegfa) Isoforms In The Testes Of Male Mice Causes Subfertility, Reduces Sperm Numbers, And Alters Expression Of Genes That Regulate Undifferentiated Spermatogonia, Ningxia Lu, Kevin M. De Avila, Debra T. Clopton, William E. Pohlmeier, Vanessa M. Brauer, Renee M. Mcfee, John S. Weber, Napoleone Ferrara, David W. Silversides, Andrea S. Cupp

Department of Animal Science: Faculty Publications

Vascular endothelial growth factor A (VEGFA) isoform treatment has been demonstrated to alter spermatogonial stem cell homeostasis. Therefore, we generated pDmrt1-Cre;Vegfa−/− (knockout, KO) mice by crossing pDmrt1-Cre mice to floxed Vegfa mice to test whether loss of all VEGFA isoforms in Sertoli and germ cells would impair spermatogenesis. When first mated, KO males took 14 days longer to get control females pregnant (P < .02) and tended to take longer for all subsequent parturition intervals (9 days; P < .07). Heterozygous males sired fewer pups per litter (P < .03) and after the first litter took 10 days longer (P < .05) to impregnate females, suggesting a more progressive loss of fertility. Reproductive organs were collected from 6-month-old male mice. There were fewer sperm per tubule in the corpus epididymides (P < .001) and fewer ZBTB16-stained undifferentiated spermatogonia (P < .003) in the testes of KO males. Testicular mRNA abundance for Bcl2 (P < .02), Bcl2:Bax (P < .02), Neurog3 (P < .007), and Ret was greater (P = .0005), tended to be greater for Sin3a and tended to be reduced for total Foxo1 (P < .07) in KO males. Immunofluorescence for CD31 and VE-Cadherin showed no differences in testis vasculature; however, CD31-positive staining was evident in undifferentiated spermatogonia only in KO testes. Therefore, loss of VEGFA isoforms in Sertoli and germ cells alters genes necessary for long-term maintenance of undifferentiated spermatogonia, ultimately reducing sperm numbers and resulting in subfertility.


Atf3 Expression In The Corpus Luteum: Possible Role In Luteal Regression, Dagan Mao, Xiaoying Hou, Heather Talbott, Robert A. Cushman, Andrea S. Cupp, John S. Davis Dec 2013

Atf3 Expression In The Corpus Luteum: Possible Role In Luteal Regression, Dagan Mao, Xiaoying Hou, Heather Talbott, Robert A. Cushman, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

The present study investigated the induction and possible role of activating transcription factor 3 (ATF3) in the corpus luteum. Postpubertal cattle were treated at midcycle with prostaglandin F2α(PGF) for 0–4 hours. Luteal tissue was processed for immunohistochemistry, in situ hybridization, and isolation of protein and RNA. Ovaries were also collected from midluteal phase and first-trimester pregnant cows. Luteal cells were prepared and sorted by centrifugal elutriation to obtain purified small (SLCs) and large luteal cells (LLCs). Real-time PCR and in situ hybridization showed that ATF3 mRNA increased within 1 hour of PGF treatment in vivo. Western blot and immunohistochemistry demonstrated …


West Coast Rock Lobster Harvest Strategy And Control Rules 2015 - 2019: A Discussion Paper, Western Australian Department Of Fisheries Dec 2013

West Coast Rock Lobster Harvest Strategy And Control Rules 2015 - 2019: A Discussion Paper, Western Australian Department Of Fisheries

Fisheries management papers

No abstract provided.


West Coast Rock Lobster Harvest Strategy And Control Rules : 2015 – 2019 : A Discussion Paper, Department Of Fisheries Dec 2013

West Coast Rock Lobster Harvest Strategy And Control Rules : 2015 – 2019 : A Discussion Paper, Department Of Fisheries

Fisheries management papers

No abstract provided.


Longevity And Mortality Of Owned Dogs In England, D. G. O'Neill, D. B. Church, P. D. Mcgreevy, P. C. Thomson, D. C. Brodbelt Dec 2013

Longevity And Mortality Of Owned Dogs In England, D. G. O'Neill, D. B. Church, P. D. Mcgreevy, P. C. Thomson, D. C. Brodbelt

Epidemiology Collection

Improved understanding of longevity represents a significant welfare opportunity for the domestic dog, given its unparalleled morphological diversity. Epidemiological research using electronic patient records (EPRs) collected from primary veterinary practices overcomes many inherent limitations of referral clinic, owner questionnaire and pet insurance data. Clinical health data from 102,609 owned dogs attending first opinion veterinary practices (n = 86) in central and southeast England were analysed, focusing on 5095 confirmed deaths.

Of deceased dogs with information available, 3961 (77.9%) were purebred, 2386 (47.0%) were female, 2528 (49.8%) were neutered and 1105 (21.7%) were insured. The overall median longevity was 12.0 years …


Fall Field Report, August–November 2013, W. Ross Silcock Dec 2013

Fall Field Report, August–November 2013, W. Ross Silcock

Nebraska Bird Review

In most ways, this was a routine fall season. Most interesting from an ongoing point of view were quite a large number of sightings of passerines at rather late dates, notably Empidonax flycatchers, wood warblers, native sparrows, tanagers, towhees, grosbeaks, and buntings. A similar phenomenon was noted among a few cold-sensitive non-passerines, such as Black and Common Terns.

One of the more significant ongoing trends is the proliferation of western and southern hummingbirds and the alteration of traditional migration and summer ranges; no fewer than 6 species were found. Calliope and Rufous Hummingbirds were seemingly more numerous in the east …


Nebraska Bird Review (December 2013) 81(4), Whole Issue Dec 2013

Nebraska Bird Review (December 2013) 81(4), Whole Issue

Nebraska Bird Review

Fall Field Report, August–November 2013 … 134

First Documented Record of Hooded Oriole (Icterus cucullatus) in Nebraska … 161

Hayden, Tristram, and a Pigeon from “Nebraska” … 165

Bridgeport Fall Field Days, September 20–22, 2013 ... 169

Index to Volume 81 ... 175

Subscription and Organization Information ... 187


Landings, Vol. 21, No. 12, Maine Lobstermen’S Community Alliance Dec 2013

Landings, Vol. 21, No. 12, Maine Lobstermen’S Community Alliance

Landings: News & Views from Maine's Lobstering Community

Landings content emphasizes science, history, resource sustainability, economic development, and human interest stories related to

Maine’s lobster industry. The newsletter emphasizes lobstering as a traditional, majority-European American lifeway with an economic and social heritage unique to the coast of Maine. The publication focuses how ongoing research to engage in sustainable, non-harmful, and non-wasteful commercial fishing practices benefit both the fishery and Maine's coastal legacy.

Maine Lobstermen’s Community Alliance (MLCA) started publication of Landings, a 24-page newsletter in January 2013 as the successor of the Maine Lobstermen’s Association (MLA) Newsletter. As of 2022, the MLCA published over 6,500 copies of …


Español A Inglés / English To Spanish: A Guide For Communication In Livestock Operations, Charles A. Shapiro, Leslie J. Johnson, John C. Fech, Paul Hay, Tom Holman, Larry Howard Dec 2013

Español A Inglés / English To Spanish: A Guide For Communication In Livestock Operations, Charles A. Shapiro, Leslie J. Johnson, John C. Fech, Paul Hay, Tom Holman, Larry Howard

University of Nebraska-Lincoln Extension: Historical Materials

Translated by: Rachel Albin, Dianne Danehey, Trevor Wright, and Alex Alpizar

Inglés/English * Cómo pronunciar la frase en Inglés * Español/Spanish * How to pronounce the phrase in Spanish

Numbers * General Communication * Locations * Weather * Colors * Days * Descriptions * Quantities * Feeding * Animal Health * Veterinary Terms * Manure Handling * Animals * General animal care


First Documented Record Of Hooded Oriole (Icterus Cucullatus) In Nebraska, Joseph Gubanyi Dec 2013

First Documented Record Of Hooded Oriole (Icterus Cucullatus) In Nebraska, Joseph Gubanyi

Nebraska Bird Review

During May 2013 a very cooperative Hooded Oriole (Icterus cucullatus) provided lots of excitement for Nebraska birders, becoming the first documented record for the species in Nebraska. The bird was first observed 25 May 2013 by Susie and Bill Daro at their residence in Garrison (Butler County). The Daros have several oriole feeders, and Baltimore and Orchard Orioles are common visitors. On the morning of May 25 they noted an unusual oriole visiting their feeders and, after checking the National Geographic Field Guide to the Birds of North America, concluded it was a Hooded Oriole. They found …


Correction [December 2013] Dec 2013

Correction [December 2013]

Nebraska Bird Review

The first Nebraska record of an Acorn Woodpecker was from Holt Co., 19–22 May 1996, not Cherry Co., as was reported on page 112 of the September 2013 issue of The Nebraska Bird Review.


Index To Volume 81 Dec 2013

Index To Volume 81

Nebraska Bird Review

Akers, D. J. 164
Albano, D. J. 164
Alberts, Byron 103, 113
Allen: Edward 4, 52, 103, 136; Jan 136
Arctos 168
Arnold, K. A. 31
Aubushon, Kathy 34
Austin, O. L. 92
Avocet, American 59, 94, 95, 109, 142, 171

. . .

Yantachka, Jen 52
Yellowlegs: Greater 10, 59, 94, 96, 109, 142, 171; Lesser 60, 94, 96, 110, 142, 171
Yellowthroat, Common 71, 97, 117, 156, 173
Young, Matt 53
Zimmerman: Jerald 34; Roy 53
Ziolkowski, D., Jr. 80
Zonotrichia leucophrys: leucophrys 158; oriantha 158


Bridgeport Fall Field Days, Janis Paseka Dec 2013

Bridgeport Fall Field Days, Janis Paseka

Nebraska Bird Review

The 2013 NOU Fall Field Days took place at the Prairie Winds Community Center in Bridgeport on Sept. 20–22. The meeting, organized by Kathy DeLara, drew a total of 50 attendees. On Friday evening Luke Hamilton led a group owling to the Bridgeport SRA and to Courthouse Rock. Field trip destinations on Saturday and on Sunday morning included Bridgeport SRA, Fleisbach WMA (aka Facus Springs), Crescent Lake NWR, Hackberry Road in Banner Co., the I-80 Exit I area in Kimball Co, Oliver Reservoir, Wildcat Hills SRA and Wind Springs Ranch. Trips were led by Kathy DeLara, Ann Duey, Cheryl Hamilton, …


Hayden, Tristram, And A Pigeon From “Nebraska”, Rick Wright Dec 2013

Hayden, Tristram, And A Pigeon From “Nebraska”, Rick Wright

Nebraska Bird Review

A passenger pigeon skin in the World Museum in Liverpool, England, was collected during an expedition to Nebraska and Dakota led by Gouverneur K. Warren between 1855 and 1857 and later cataloged by geologist and naturalist Ferdinand V. Hayden. For a time it was in the collection of Henry Baker Tristram, a famous naturalist and a founder of the British Ornithologists’ Union. Passenger pigeons were once “quite abundant” along the Missouri River.


Subscription And Organization Information [December 2013] Dec 2013

Subscription And Organization Information [December 2013]

Nebraska Bird Review

The Nebraska Bird Review is published quarterly by the Nebraska Ornithologists' Union, Inc., as its official journal, and is sent to members not in arrears of dues. Annual subscription rates (on a calendar-year basis only): $25 in the United States and $35 in all other countries, payable in advance. Single copies are $7 each, postpaid, in the United States and $9 elsewhere. Send orders for back issues to Anita Breckbill, NOU Librarian, c/o Music Library, WMB 30, University of Nebraska, Lincoln, NE 68588-0101.

Memberships in the NOU (on a calendar-year basis only): Active Household (one or more people) $25; Sustaining …


First State Record And Interdiction For The Wood Slave, Hemidactylus Mabouia (Moreau De Jonnès 1818) (Gekkonidae), In Maryland, Usa., Louis A. Somma, William L. Grogan Jr. Dec 2013

First State Record And Interdiction For The Wood Slave, Hemidactylus Mabouia (Moreau De Jonnès 1818) (Gekkonidae), In Maryland, Usa., Louis A. Somma, William L. Grogan Jr.

Papers in Herpetology

The Wood Slave, Hemidactylus mabouia (Moreau de Jonnès 1818; Fig. 1), is indigenous to Africa south of the Sahara, with nonindigenous populations established in Cape Verde, Mexico, much of Central and South America, numerous localities in the Caribbean, perhaps Madagascar, and at least 21 counties in Florida, USA (Carranza and Arnold 2006; Kraus 2009; Krysko et al. 2011a, 2011b; Meshaka 2011; Powell and Henderson 2012). Recently, H. mabouia has successfully invaded temperate regions of southern Africa and northern peninsular Florida (Alexander and Marais 2007, Krysko and Somma 2007).

On 20 December 2012, Brian R. Grogan and Shane Forsythe collected and …


Arkansas Animal Science Department Report 2013, David L. Kreider, Paul Beck Dec 2013

Arkansas Animal Science Department Report 2013, David L. Kreider, Paul Beck

Arkansas Agricultural Experiment Station Research Series

No abstract provided.


A Northward Range Extension Of The Hispid Cotton Rat (Sigmodon Hispidus) In Missouri, Cody W. Thompson, Elmer J. Finck Dec 2013

A Northward Range Extension Of The Hispid Cotton Rat (Sigmodon Hispidus) In Missouri, Cody W. Thompson, Elmer J. Finck

The Prairie Naturalist

The hispid cotton rat (Sigmodon hispidus) is a widely distributed rodent with a geographic range extending from north-central Mexico to southern Nebraska and central Virginia and from southeastern Arizona east to Florida (Carleton et al. 1999, Peppers and Bradley 2000, Wilson and Reeder 2005) with isolated populations in Arizona (Bradley et al. 2012) and California (Clark 1972). Range expansions for the species have been well documented (Clark 1972, Farney 1975, Benedict et al. 2000, Wright et al. 2010, Wills et al. 2011). The majority of these range expansions have occurred across the Central Plains during the 20th century …


Novel Applications Of Multivariate Methods For Exploring Personality In African Elephants, Shilo Kimberly Felton Dec 2013

Novel Applications Of Multivariate Methods For Exploring Personality In African Elephants, Shilo Kimberly Felton

Masters Theses & Specialist Projects

Investigators have shown that elephants exhibit consistent individual differences in behavior by rating elephants using personality adjectives. These adjectives, however, are not based on pre-defined measurements of the behaviors performed. Instead, they are based on the observers’ interpretations of an animal’s behavioral patterns, therefore making them subject to observer bias. Furthermore, elephants have a capacity for learning; thus, they may alter their behavioral patterns over time. This behavioral plasticity in itself might be a way of measuring consistent behavioral differences among individuals. With this in mind, I approached elephant personality as a multivariate problem. I used behavioral observations collected from …


Landscape Genetics Of The Marbled Salamander (Ambystoma Opacum) At Mammoth Cave National Park, James Kyle Martin Dec 2013

Landscape Genetics Of The Marbled Salamander (Ambystoma Opacum) At Mammoth Cave National Park, James Kyle Martin

Masters Theses & Specialist Projects

Habitat connectivity is important to maintain in order to prevent loss of genetic diversity, reduce inbreeding depression, and decrease extinction risk in threatened or endangered species. Here I present a landscape genetics study on marbled salamanders (Ambystoma opacum) in highly connected forested habitat at Mammoth Cave National Park. This investigation of gene flow among ponds within a mostly continuous landscape provides data that can be compared with patterns observed in more fragmented landscapes. These comparisons can provide a means of investigating the separate effects of structural and functional habitat connectivity on amphibian genetic population structure. Structural connectivity refers to the …