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Full-Text Articles in Animal Sciences

Application Of Lc-Ms/Ms In Identification Of Toxin In The Causative Gastropod And Victim Samples, Chun-Lan Lin, Cheng-Hong Hsieh, You-Liang Hsieh, Yi-Cheng Tai, Deng-Fwu Hwang Dec 2013

Application Of Lc-Ms/Ms In Identification Of Toxin In The Causative Gastropod And Victim Samples, Chun-Lan Lin, Cheng-Hong Hsieh, You-Liang Hsieh, Yi-Cheng Tai, Deng-Fwu Hwang

Journal of Marine Science and Technology

Food paralytic poisoning incident was detected in the gastropod Nassariidae Niotha clathrata and Zeuxis scalaris occurred in December 2012 in Kaohsiung, Taiwan. The toxin was purified from each remain of cooked gastropod and victim tissues by ultrafiltration using a YM-3 membrane, followed by C18 cartridge column purification. Based on analyses by LC-MS/MS, the causative agent of gastropod food poisoning was identified as tetrodotoxin. The toxicity of remain of cooked sample was 245 MU/g (1MU = 0.178 µg TTX) for N. clathrata and 203 MU/g for Z. scalaris. The blood and excrements of victim was shown to contain TTX of 0.13 …


Comparative Morphological Study Of Otoliths In Taiwanese Anguilliformes Fishes, Abbie K. Chulin, Hong-Ming Chen Dec 2013

Comparative Morphological Study Of Otoliths In Taiwanese Anguilliformes Fishes, Abbie K. Chulin, Hong-Ming Chen

Journal of Marine Science and Technology

Otoliths are calcareous structures found in the labyrinth membrane in the otic capsules of teleost fishes and are constituted of three pairs: the sagittae, lapilli and asterisci. They have long been recognized as being species specific. A comparative morphology of the sagittae, asterisci and lapilli among six eel species of Anguilliformes belonging to the Family Congridae, Muraenesocidae and Muraenidae was studied. A total of 64 specimens belonging to Bathyconger wallacei (n = 23), Conger japonicus (n = 9), Muraenesox cinereus (n = 10), Echidna polyzona (n = 4), Gymnothorax eurostus (n = 4) and Gymnothorax reticularis (n = 14) were …


The Molecular Phylogeny Of Genus Hemigobius (Teleostei: Gobiidae), With The Comfirmation Of Validity Of Hemigobius Crassa (Herre, 1945), Shih-Pin Huang, I-Shiung Chen, Kwang-Tsao Shao Dec 2013

The Molecular Phylogeny Of Genus Hemigobius (Teleostei: Gobiidae), With The Comfirmation Of Validity Of Hemigobius Crassa (Herre, 1945), Shih-Pin Huang, I-Shiung Chen, Kwang-Tsao Shao

Journal of Marine Science and Technology

The genus Hemigobius Bleeker, 1874 is has been regarded as only two valid species in previous study including H. hoevenii (Bleeker, 1851) and H. mingi (Herre, 1936). Traditionally, the both Vaimosa crassa Herre, 1945 and Mugilogobius obliquifasciatus Wu and Ni, 1985 found from Chinese and Taiwanese waters had ever been regarded as the junior synonyms of H. hoevenii. However, our present study have been confirm that H. crassa collected from Hong Kong and Taiwan is a valid species. The H. crassa can be well distinguished from H. hoevenii based on several morphological features. The molecular phylogenetic analysis firstly based on …


A New Species Of Genus Pseudogobius Popta (Teleostei: Gobiidae) From Brackish Water Of Taiwan And Southern China, I-Shiung Chen, Shih-Pin Huang, Kuang-Ying Huang Dec 2013

A New Species Of Genus Pseudogobius Popta (Teleostei: Gobiidae) From Brackish Water Of Taiwan And Southern China, I-Shiung Chen, Shih-Pin Huang, Kuang-Ying Huang

Journal of Marine Science and Technology

A new species of fat-nose goby genus Pseudogobius taijiangensis n. sp. is firstly described herein and was collected from brackish waters of Taiwan and southern China. P. taijiangensis n. sp. can be well distinguished from other congeners by the unique combinations of the following features: (1) fin rays: 2nd dorsal fin rays modally 7, anal fin rays modally 7, pectoral fin rays modally 17 and first dorsal fin rounded and lacking filaments; (2) squamation: lateral body with large ctenoid scales, longitudinal scale rows 26-28, predorsal scales modally 8; and (3) specific coloration: body with some black thin longitudinal lines; rear …


A New Species Of Cyprinid Fish Of Genus Opsariichthys From Ky Cung-Bang Giang River Basin, Northern Vietnam With Notes On The Taxonomic Status Of The Genus From Northern Vietnam And Southern China, Thien Quang Huynh, I-Shiung Chen Dec 2013

A New Species Of Cyprinid Fish Of Genus Opsariichthys From Ky Cung-Bang Giang River Basin, Northern Vietnam With Notes On The Taxonomic Status Of The Genus From Northern Vietnam And Southern China, Thien Quang Huynh, I-Shiung Chen

Journal of Marine Science and Technology

Opsariichthys duchuunguyeni, a new species of cyprinid fish from Ky Cung – Bang Giang river basin, northern Vietnam, is described. It is characterized by the following unique combination of features: short maxillary extending; 12-15 rounded tubercles on lower jaw; 11-12 crossbars on body; pectoral fin reaching the pelvic fin origin in adult male; lateral-line scales comprising 41; scales above lateral-line 8; scales below lateral-line 3. Moreover, the genetic differentiation and a diagnostic key of the new species and other congeners are given in this paper for discussion about taxonomic status of the genus Opsariichthys from Vietnam and nearby geographical regions, …


Reproductive Biology Of Intertidal Frillfin Goby, Bathygoboius Fuscus In Keelung, Taiwan, Yu-Hai Kong, I-Shiung Chen Dec 2013

Reproductive Biology Of Intertidal Frillfin Goby, Bathygoboius Fuscus In Keelung, Taiwan, Yu-Hai Kong, I-Shiung Chen

Journal of Marine Science and Technology

The reproductive biology of intertidal, black brown frillfin goby, Bathygobius fuscus (Rüppell) have been surveyed from the coastal region of Keelung, Taiwan. The species always belongs to the dominant species found in the fish community of the tidal pools for different seasons. The overall fecundity of adult female is from 2335 to 13332 eggs with the average of 5605 eggs with average eggs-size is 0.34 ± 0.06 mm. The female minimum measure body-size would be 40.4 mm SL. The higher reproductive season can be well observed from female GSI (Gonadosomatic Index) during May to September. During that time, there may …


The Diel Dynamics Of Ciliate Community In A Tide-Pool, Chien-Fu Chao, Bo-Wei Wang, Chao-Hsiung Cheng, Kuo-Ping Chiang Dec 2013

The Diel Dynamics Of Ciliate Community In A Tide-Pool, Chien-Fu Chao, Bo-Wei Wang, Chao-Hsiung Cheng, Kuo-Ping Chiang

Journal of Marine Science and Technology

We investigated the diel cycle mechanism of a tide-pool ciliate community of the northeastern coast of Taiwan. The investigation was conducted on 17th-18th June (typical summer weather), and on 23th -24th July 2013 (10 days after a typhoon). In June, the tide-pool ecosystem was an independent and the excystment of ciliates in the tide-pool caused a dramatic increase in population abundance, as well as composition change. In July, there was no significant difference between the species composition in the tide-pool and that in the surrounding coastal water. However, the abundance of ciliates in the tide-pool was lower due to the …


A New Parapercis Species From The Dongsha Island, South China Sea With Comments On A New Record From Taiwan, I-Shiung Chen, Tsung-Hsien Tsai, Shao-Liang Hsu Dec 2013

A New Parapercis Species From The Dongsha Island, South China Sea With Comments On A New Record From Taiwan, I-Shiung Chen, Tsung-Hsien Tsai, Shao-Liang Hsu

Journal of Marine Science and Technology

The sandperch genus, Parapercis Bleeker, 1863 is the most diverse group of Family Pinguipedidae in the perciforms. One unusual specimen of sandperches recently collected from the Dongsha Island (Pratas Island), South China Sea from 2011 expedition belonging to Taiwan, the Republic of China is turned to a new species as Parapercis dongshaensis n. sp. closely related to the newly recorded species, Parapercis okamurai Kamohara, 1960 which also firstly collected from Taiwanese waters would been also documented herein. The new species, Parapercis dongshaensis n. sp., can be well distinguished from other congeners by the following unique combination of features: (1) fins: …


Genetic Diversity Of Pyramimonas From Ryukyu Archipelago, Japan (Chlorophyceae, Pyramimonadales), Shoichiro Suda, Mohammad Azmal Hossain Bhuiyan, Daphne Georgina Faria Dec 2013

Genetic Diversity Of Pyramimonas From Ryukyu Archipelago, Japan (Chlorophyceae, Pyramimonadales), Shoichiro Suda, Mohammad Azmal Hossain Bhuiyan, Daphne Georgina Faria

Journal of Marine Science and Technology

The genus Pyramimonas Schmarda was traditionally described on observations of its periplast and internal structure of different flagellar apparatus or eyespot orientations and currently comprises of ca. 60 species that are divided into six subgenera: Pyramimonas, Vestigifera, Trichocystis, Punctatae, Hexactis, and Macrura. In order to understand the genetic diversity and phylogenetic relationships of genus Pyramimonas members, we analyzed nuclear SSU rDNA molecular data from 41 strains isolated from different locations of the Ryukyu Archipelago. Phylogenetic analyses revealed that strains could be segregated into six clades, four of which represented existing subgenera: Pyramimonas, Vestigifera McFadden, Trichocystis McFadden, and Punctatae McFadden, and …


Life History Of The Copepod Paramphiascella Sp. Affected By Hydrothermal Vent Effluents, Hans-Uwe Dahms, Li-Chun Tseng, Jiang-Shiou Hwang Dec 2013

Life History Of The Copepod Paramphiascella Sp. Affected By Hydrothermal Vent Effluents, Hans-Uwe Dahms, Li-Chun Tseng, Jiang-Shiou Hwang

Journal of Marine Science and Technology

Toxicity of Hydrothermal vent (HV) effluents was tested by investigating the effect on growth and reproduction of the copepod Paramphiascella sp. which was collected and subsequently cultured from localities nearby a shallow marine HV at Kueishan Tao Island, Taiwan. Ontogenetic stages (nauplii, copepodids, and adults) were exposed to a range of concentrations of HV effluents in a static renewal culture system. In a first of two experiments we tested the survivorship of these in HV effluent dilutions from 1 to 50%. HV effluents significantly reduced the survivorship of the naupliar stages at concentrations >5% for Paramphiascella sp. (p < 0.01) and all nauplii died at concentrations of 25% and 50%. Copepodids were significantly letally affected at concentrations >5% (p < 0.01) and all died at 50% (p < 0.01). Developmental duration in Paramphiascella was showing a trend of developmental delay in both phases, in the naupliar and in the copepodid phase. Mortality showed a greater sensitivity to chemical exposure than development time. Among both traits were early developmental stages of Paramphiascella sp. more sensitive to HV effluents than advanced stages. We showed that Paramphiascella sp. was a useful test organism in the monitoring of life cycle as well as acute effects of HV effluents being present for millions of years and providing unique ecosystems. Mortality was a useful toxicological endpoint whereas developmental duration was not.


Gall Polymorphism Of Coral-Inhabiting Crabs (Decapoda, Cryptochiridae): A New Perspective, Tsui-Ping Wei, Hon-Cheng Chen, Ying-Chou Lee, Min-Li Tsai, Jiang-Shiou Hwang, Shao-Hung Peng, Yuh-Wen Chiu Dec 2013

Gall Polymorphism Of Coral-Inhabiting Crabs (Decapoda, Cryptochiridae): A New Perspective, Tsui-Ping Wei, Hon-Cheng Chen, Ying-Chou Lee, Min-Li Tsai, Jiang-Shiou Hwang, Shao-Hung Peng, Yuh-Wen Chiu

Journal of Marine Science and Technology

Coral-inhabiting gall crabs are either obligate symbionts or parasitic associates with their host corals. They form a variety of galls/pits inside the skeleton of living corals. Nine genera of gall crabs on several scleractinian corals were used to test the hypothesis that galls vary among cryptochirid genera. Phylogenetic and morphometric observations were combined to analyze the possible evolutionary significance of gall construction. A high degree of conservation of gall shapes was observed in relation to the gall crabs’ phylogeny. Gall/pit morphology and fidelity was studied in each of the different species of gall crabs. In addition, the correlation analysis results …


Ichthyoplankton Community Associated With Oceanic Fronts In Early Winter On The Continental Shelf Of The Southern East China Sea, Yi-Chen Wang, Wen-Yu Chen, Yi Chang, Ming-An Lee Dec 2013

Ichthyoplankton Community Associated With Oceanic Fronts In Early Winter On The Continental Shelf Of The Southern East China Sea, Yi-Chen Wang, Wen-Yu Chen, Yi Chang, Ming-An Lee

Journal of Marine Science and Technology

The study of ichthyoplankton community associated with oceanic features on the continental shelf of southern East China Sea (ECS) was conducted in early winter from 2006 to 2009. The species composition of the ichthyoplankton and its spatial distribution was classified and compared with oceanic front. A total number of 1,809 fish larvae individuals were sampled from 5 field surveys, and represented 76 families and 137 species. Using the Bray-Curtis dissimilarity, the abundance of species found to exceed 0.5% of the total sample from 36 stations were classified into four groups: China Coastal Group, Mixed Shelf Group, Taiwan Strait Group, and …


A New Freshwater Goby Of Rhinogobius (Teleostei: Gobiidae) From Hainan Island, Southern China, I-Shiung Chen, Peter J. Miller Dec 2013

A New Freshwater Goby Of Rhinogobius (Teleostei: Gobiidae) From Hainan Island, Southern China, I-Shiung Chen, Peter J. Miller

Journal of Marine Science and Technology

A new freshwater goby of Rhinogobius Gill (1859) was collected from the Wangchuang River basin, Hainan Island, southern China. The new species, Rhinogobius sangenloensis, can be well distinguished from other congeners by a combination of the following features: second dorsal-fin rays modally I/8; anal-fin rays I/7-8; pectoral-fin rays modally 17; longitudinal scales 25-27; predorsal scales 9-11; vertebrae 26; body with 3-4 longitudinal, discontinuous orange to brown or brownish black bars and stripes; cheek with 2 grayish black spots in male; pectoral-fin base in male with 2 oblong black bars; caudal fin with an orange mark on lower 1/3 region in …


A New Genus Of Hemigobius Generic Group Goby Based On Morphological And Molecular Evidence, With Description Of A New Species, Shih-Pin Huang, Jaafar Zeehan, I-Shiung Chen Dec 2013

A New Genus Of Hemigobius Generic Group Goby Based On Morphological And Molecular Evidence, With Description Of A New Species, Shih-Pin Huang, Jaafar Zeehan, I-Shiung Chen

Journal of Marine Science and Technology

Wuhanlinigobius, a new genus of Hemigobius generic group would been established and assigned from Mugilogobius polylepis Wu and Ni, 1985. Mugilogobius polylepis has been regarded as belong to genus Eugnathogobius based on lacking head pores and representing longitudinal sensory papillae in previous taxonomic study. However, we compared the osteological features of Mugilogobius polylepis Wu and Ni, 1985 and Eugnathogobius microps Smith, 1931 as well as the molecular phylogenetic analysis based on the mtDNA ND5, Cyt-b genes and D-loop region. The molecular phylogenetic tree including 9 related Hemigobius generic group reveal that this new genus represents an independent clade which is …


Alternative Assessment Methods Applied To The Hairtail (Trichiurus Lepturus) Stock In The Aru Sea As An Example, Chao-Hsiung Cheng, Tsuyoshi Kawasaki, Kuo-Ping Chiang, Chuan-Hung Ho Dec 2013

Alternative Assessment Methods Applied To The Hairtail (Trichiurus Lepturus) Stock In The Aru Sea As An Example, Chao-Hsiung Cheng, Tsuyoshi Kawasaki, Kuo-Ping Chiang, Chuan-Hung Ho

Journal of Marine Science and Technology

The Aru Sea off Indonesia is a major fishing ground for Taiwanese distant-water otter trawlers. The hairtail, an economically important demersal fish, is abundant in that area. The assessment methodology in this paper extends previous studies on hairtail fisheries by considering migration within the fishing grounds. This model is based on conventional Leslie-Delury method but makes different assumptions on stock movement and the relationship between stock abundance and catch per unit effort. It was applied to retrospective data from the hairtail fisheries in the Aru Sea. In addition, the depletion method was used to estimate the abundance of hairtail and …


Purification And Characterization Of Acidic Protease From Aspergillus Oryzae Bcrc 30118, Li-Jung Yin, Ya-Hui Chou, Shann-Tzong Jiang Feb 2013

Purification And Characterization Of Acidic Protease From Aspergillus Oryzae Bcrc 30118, Li-Jung Yin, Ya-Hui Chou, Shann-Tzong Jiang

Journal of Marine Science and Technology

The acidic protease was purified from 4-day cultivation of Aspergillus oryzae BCRC 30118 by DEAE Sephacel and Sephacryl S-200 HR chromatographs. The specific activity, yield and purification fold were 117.62 kU/mg, 15.1% and 6.6, respectively. The molecular weight (M) was 41.0 kDa, while the optimal pH and temperature were 3.0 and 60°C, respectively. It was stable at pH 3.0-6.0 and 4-35°C. However, it was inhibited by Fe2+, Hg2+, Fe3+ and pepstatin A, and slightly by leupeptin and TPCK. According to substrate specificity and inhibitor study, it was a cysteine protease with activation energy of 37.5 kcal/mol. Its Km, Vmax, Kcat …


Decadal Variation In Egg Abundance Of A Mesopelagic Fish, Maurolicus Japonicus, In The Japan Sea During 1981-2005, Tadanori Fujino, Tsuneo Goto, Tsuyoshi Shimura, Hiroki Yasuma, Yongjun Tian, Hideaki Kidokoro, Shinya Masuda, Kazushi Miyashita Feb 2013

Decadal Variation In Egg Abundance Of A Mesopelagic Fish, Maurolicus Japonicus, In The Japan Sea During 1981-2005, Tadanori Fujino, Tsuneo Goto, Tsuyoshi Shimura, Hiroki Yasuma, Yongjun Tian, Hideaki Kidokoro, Shinya Masuda, Kazushi Miyashita

Journal of Marine Science and Technology

Egg abundance of Maurolicus japonicus, a dominant mesopelagic fish in the Japan Sea, was analyzed during 1981-2005 as an index of the spawning-adult abundance. There was a major change in egg abundance around 1988/1989, with negative anomalies occurring during 1981-1988 and positive anomalies occurring from l989. At about the same time (1986/1987), the Tsushima Current shifted from cold to warm conditions. Environmental indices were found to be positively correlated with egg abundance two years later. Our results suggest that the abundance of M. japonicus is affected by climatic regime shifts, and that it could be used as an indicator species.