Animal Sciences Commons^™

The Effect Of Silver Nanoparticles On Synaptic Responses In The Lateral Giant Escape Circuit Of The Crayfish, Samantha N. Adkins

Theses, Dissertations and Capstones

The lateral giant neuron in the crayfish is a neuron that controls the reflexive escape response; the context and experience-dependent tuning of this response is critical for survival. Serotonin modulates synaptic responses in this neuron, and social experiences (stress) changes the modulatory role of serotonin. I investigated the mechanisms of induced synaptic response changes and serotonergic modulatory changes after silver nanoparticle exposure to ask if 1) contaminant induced stress changes serotonergic modulation in a manner consistent with social stress and 2) shed light on potential neurotoxic effects of widely used, but poorly understood nanomaterials. Our data show that stress induced …

Shape Evolution And Sexual Dimorphism In The Mandible Of The Dire Wolf, Canis Dirus, At Rancho La Brea, Alexandria L. Brannick

Theses, Dissertations and Capstones

The Rancho La Brea tar pits are a classic fossil lagerstätten, famous for the deposition of episodic accumulations of large numbers of fossils over short intervals in the late Pleistocene. I analyzed 157 Canis dirus (dire wolf) hemi-mandibles from Rancho La Brea through a 2D landmark-based morphometric analysis to test for size and shape changes through time. I scored 16 landmarks on each mandible gathered from four pits of different ages: 61/67 (~13-14 thousand years ago [ka]), 13 (~17-18 ka), 2051 (~26 ka), and 91 (~28 ka). Analyses indicate size does change through time, and shares a broad correlation with …

Subthreshold Toxic Effects Of Atrazine And Three Degradates On Behavior In Procambarus Clarkii, Carol B. Starkey

Theses, Dissertations and Capstones

Atrazine is among the most heavily applied pesticides worldwide, and recent evidence suggests that it may be unsafe at environmental levels. It is a known endocrine disruptor and a suggested neurotoxin. The US EPA Maximum Contaminant Level (MCL) is 3 μg/L for human ingestion and 200 μg/L for limited human exposure. Several environmentally persistent degradates have been identified, including deethylatrazine (DEA), deisopropylatrazine (DIA) and hydroxyatrazine (HA). No MCLs have been established for these degradates, although some are suggested toxins. Thus, there remains concern for the risk associated with the presence of atrazine and its degradates in the environment. Currently little …

Examining The Effects Of Liming On Gyrinophilus Porphyriticus With A Comparison Of Multiple Sampling Methods, Elise Marie Edwards

Theses, Dissertations and Capstones

Environmental acidification is affecting stream-associated species around the globe. As a result, different management strategies have been implemented to deal with acidification resulting from acid rain and acid mine drainage. Adding limestone to streams attempts to reverse the effects of acidification. While liming increases the pH of many acidic environments, studies involving the effects of liming on different animal species have shown mixed results. In this study, I examined the effects of liming on Gyrinophilus porphyriticus (the Spring Salamander) abundance and I also determined the best method of detection for G. porphyriticus. From June 10th to September 1st 2013, I …

Using Ailuropoda Melanoleuca As A Model Species For Studying The Ecomorphology Of Paranthropus, Ryan Andrew King

Theses, Dissertations and Capstones

Giant pandas (Ailuropoda melanoleuca) have cranial morphology similar to the extinct hominin genus Paranthropus which makes them an excellent model species when studying Paranthropus diet. Both species have wide skulls with flared zygomatic arches adapted for chewing. To gain insight into possible food sources of Paranthropus, I investigated the giant panda’s specialized diet of bamboo. The toughness, hardness, and stiffness of various bamboo species was determined to assess mechanical challenges facing giant pandas during feeding. Bamboo is thought to be tough, but studies on such properties and how they apply to mastication of giant pandas are largely …

The Natural Life History Of Cambarus (Puncticambarus) Smilax, The Greenbrier Crayfish, Paul William Hughes

Theses, Dissertations and Capstones

The natural life history of Cambarus (Puncticambarus) smilax, the Greenbrier Crayfish, was studied in West Fork of the Greenbrier River and in Thorny Creek, a tributary of the main stem of the Greenbrier River. The Greenbrier Crayfish gets its name from the Greenbrier River watershed where it is thought to occur exclusively. Among described members of the subgenus Puncticambarus, C. smilax is a sister taxon most similar to Cambarus (Puncticambarus) robustus. Monthly collections were made within the two study sites, from August 2010 to July 2011. Collecting techniques included dip-netting, seine-netting, and hand collecting. Cambarus smilax and all other species …

Natural History Of A Population Of Wood Turtles (Glyptemys Insculpta) In West Virginia, Jessica Curtis

Theses, Dissertations and Capstones

The Wood Turtle (Glyptemys insculpta) is listed as vulnerable in West Virginia and only occurs in the Eastern Panhandle. The study population is unique in West Virginia because it occurs at a much higher mean elevation than other Wood Turtle populations in the state. The primary objective of this study was to determine if, and to what extent, altitude affected this population’s ecology. Turtles were located with opportunistic surveys and captured by hand. I located 31 turtles: 18 males, and 13 females. Turtles were captured a total of 68 times. Ratio of males to females did not differ significantly from …

Assessing Grazing Impacts On A Tropical Dry Forest System In Madagascar Through Vegetation, Satellite Image, Lemur Occupancy, And Acoustic Analysis, Lyndsay Lee Rankin

Theses, Dissertations and Capstones

The ability to assess the quality or ecological value of a landscape is a useful tool in effective conservation and natural resource management. Ecosystem biodiversity and functionality are reduced when human activities cause habitat alteration and/or fragmentation. Compromised habitats are unlikely to support naturally occurring communities or healthy ecosystem functions. Conservation scientists have historically concentrated their efforts on large, intact habitats with little human disturbance; the conservation value of small and/or disturbed areas has been comparatively overlooked. One limitation may be a lack of tools to identify disturbed areas with conservation value. Techniques that allow researchers to assess the habitat …

Etudes On Iurids, Vi. Further Revision Of Calchas Birula, 1899 (Scorpiones: Iuridae), With A Description Of A New Genus And Two New Species., Ersen Aydın Yağmur, Michael E. Soleglad, Victor Fet, František Kovařík

Euscorpius

Several new Calchas populations are studied from Turkey, including specimens from Hakkari Province, which borders the northern edge of Iraq. A new genus, Neocalchas, gen. nov., and two new species, Calchas anlasi, sp. nov. and C. kosswigi, sp. nov., are described. The two genera, Calchas and Neocalchas, are diagnosed by their hemispermatophore structure, chelal finger dentition, telson morphometrics and setation, and trichobothrial topology of the pedipalp chela. Genus Calchas Birula, 1899 includes four species: C. anlasi, sp. nov., C. birulai, C. kosswigi, sp. nov., and C. nordmanni; genus …

The First Molecular Phylogeny Of Buthidae (Scorpiones), Victor Fet, Benjamin Gantenbein, Alexander Gromov, Graeme Lowe, Wilson R. Lourenço

Euscorpius

The first partial phylogeny of family Buthidae (17 genera) is presented, based on molecular data (16S rRNA mitochondrial DNA). The strong support for a monophyletic Old World group of 13 genera (mainly Palearctic desert forms) is demonstrated, while representative genera from Madagascar (Grosphus) and Southeast Asia (Lychas) group outside, as well as New World genera Centruroides and Rhopalurus. A very strong support is observed for the first time for three groups of Old World genera: (a) Compsobuthus, Mesobuthus, Liobuthus, Kraepelinia; (b) Hottentotta, Buthacus; (c) Orthochirus, Anomalobuthus. Phylogenetic …

Morphology Analysis Supports Presence Of More Than One Species In The “Euscorpius Carpathicus” Complex (Scorpiones: Euscorpiidae), Victor Fet, Michael E. Soleglad

Euscorpius

We investigate a number of scorpion populations from southern and central Europe, commonly classified under a “catch-all” name of Euscorpius carpathicus (L., 1767). This species includes a high number of described subspecies but its composition is not resolved. The detailed morphology analysis in the present paper includes a number of new characters, in particular individually mapped external patellar accessory trichobothria. It suggests that several clearly separated lineages are present. E. carpathicus (L.) is restricted here to geographically marginal populations from Romania (terra typica), which exhibit loss of one trichobothrium in the patellar series em (= 3). Another lineage (Austria, Croatia, …

Two New Andean Species Of Brachistosternus Pocock (Scorpiones: Bothriuridae), Jose A. Ochoa, Louis E. Acosta

Euscorpius

Two new Andean species of the genus Brachistosternus Pocock from southern Peru and northern Chile are described: B. titicaca sp. n. from the Titicaca basin (Departamento Puno, Peru), at 3850-3900 m, and B. quiscapata sp. n. from Departamento Tacna (Peru) and Provincia Arica (Chile), at 3100-3400 m. Both species belong to the subgenus Brachistosternus (Leptosternus) Maury. They are related to the Argentinean species B. (L.) intermedius Lönnberg and B.(L.) montanus Roig Alsina. Comparisons between these four species are provided, including a combination of characters: length/width ratio of metasomal segment V, number of setae of metasomal segment V and tarsus III, …

Etudes On Iurids, V. Further Revision Of Iurus Thorell, 1876 (Scorpiones: Iuridae), With A Description Of A New Genus And Two New Species, Michael E. Soleglad, Victor Fet, František Kovařík, Ersen Aydın Yağmur

Euscorpius

Iurus populations from the Aegean area are studied, including the Greek islands of Crete, Karpathos, Kythira, Rhodes, and Samos. A new genus, Protoiurus gen. nov., and two new species, Protoiurus rhodiensis sp. nov. and P. stathiae sp. nov., are described. The two genera, Iurus and Protoiurus, are diagnosed by their hemispermatophore structure; a cladistic analysis based on this structure is presented. Genus Iurus Thorell, 1876 includes three species: I. dekanum, I. dufoureius, and I. kinzelbachi; genus Protoiurus includes five species: P. asiaticus comb. nov., P. kadleci comb. nov., P. kraepelini comb. nov., P. rhodiensis sp. nov., and P. …

Euscorpiops Neradi Sp. N. From Thailand (Scorpiones: Euscorpiidae: Scorpiopinae), František Kovařík, Jana Plíšková, František Šťáhlavský

Euscorpius

Euscorpiops neradi sp. n. from Thailand is described and compared with other species of the genus Euscorpiops Vachon, 1980. It is the smallest species of the genus, with total length about 25 mm in both sexes. In E. neradi sp. n. external trichobothria on the patella number 16 (5 eb, 2 esb, 2 em, 3 est, 4 et) and ventral trichobothria on the patella number 6. Pedipalp fingers are flexed in males and straight in females.

Alloscorpiops Citadelle Sp. N. From Thailand (Scorpiones: Euscorpiidae: Scorpiopinae)., František Kovařík

Euscorpius

Alloscorpiops citadelle sp. n. from Thailand is described and compared with A. anthracinus (Simon, 1887). A. citadelle sp. n. is characterized mainly by a higher number of trichobothria on the patella, which has 29–34 external (23–24 in A. anthracinus) and 19–21 ventral (15–19 in A. anthracinus) trichobothria.

Evolution Of Scorpion Orthobothriotaxy: A Cladistic Approach, Michael E. Soleglad, Victor Fet

Euscorpius

This study presents a cladistic analysis of the derivation of orthobothriotaxic patterns in scorpions. Included in this analysis are the original three orthobothriotaxic patterns defined by Vachon (1972, 1974), the pattern of the unique scorpion Pseudochactas ovchinnikovi Gromov, 1998, and two trichobothrial patterns of fossil scorpions, the Upper Carboniferous palaeopisthacanthids and the Lower Cretaceous archaeobuthids. An overview of all fossil scorpion material where trichobothria are reported is presented in detail. The approach used in this analysis is to model the existence of an individual trichobothrium, adopting the ‘absence of’, ‘petite size’ and ‘full size’ as incremental stages of a trichobothrium’s …

Contributions To Scorpion Systematics. I. On Recent Changes In High-Level Taxonomy, Victor Fet, Michael E. Soleglad

Euscorpius

Prendini & Wheeler (2005) criticized the methods of phylogenetic analyses by Soleglad, Fet, and their coauthors, and executed an unprecedented taxonomic action: without analyzing any of these taxa, they performed a wholesale synonymization of four parvorders, eight superfamilies, one family, 11 subfamilies, eight tribes, two subtribes, and three genera (in total, 37 taxa) of scorpions, and made other taxonomic changes. No alternative new classification has been proposed (instead, they revert to a previous classification), and no results of original work on this subject by Prendini & Wheeler (2005) have been presented. Here, we reverse all taxonomic changes performed by Prendini …

Etudes On Iurids, Ii. Revision Of Genus Calchas Birula, 1899, With The Description Of Two New Species (Scorpiones: Iuridae), Victor Fet, Michael E. Soleglad, František Kovařík

Euscorpius

The relict, phylogenetically important scorpion genus Calchas Birula, 1899 (Iuridae) remained monotypic since its description. Its sole species, Calchas nordmanni Birula, 1899, was known only from northeastern Turkey until Kinzelbach (1980) published first records from southern and southeastern Turkey. A few more localities have been reported from Turkey; the species was also found on two Greek islands, Samos and Megisti. We analyzed significant material (63 specimens, including a previously unpublished large series from Naturhistorisches Museum Wien), and concluded that three distinct, disjunct species exist rather than one widespread species as previously thought. Two new species are described: Calchas birulai sp. …

Ecological Niche Modeling Of Two Scorpion Species Mesobuthus Eupeus (C. L. Koch, 1839) And M. Phillipsii (Pocock, 1889) From The Iranian Plateau And Zagros Region (Arachnida: Scorpiones), Omid Mirshamsi

Euscorpius

Ecological niche modeling has allowed several advances in the process of species delimitation. In the present study, I used this method to evaluate the climatic divergence between two scorpion species, Mesobuthus eupeus and M. phillipsii. The ecological niche models (ENMs) were created based on presence-only data with the maximum entropy method. The created models, results of spatial analysis (PCA and Hotelling discriminant), and an identity test suggested that the divergence between these two species is associated with significant divergence in their ecological niches. The results of this study provide additional support for the taxonomic validity the studied species.

A New Species Of Tityus C. L. Koch, 1836 (Scorpiones: Buthidae) From Dominican Amber, Wilson R. Lourenço

Euscorpius

Tityus azari sp. n., a new species of fossil scorpion belonging to the genus Tityus C. L. Koch, 1836 is described based on a specimen in amber from the Dominican Republic. Although the new species can be associated with the extant fauna of the Neotropical region, it presents some particular morphological features such as the presence of sharp denticles on the edge of pedipalp fingers. Due to the incompleteness of the specimen it cannot be assigned to any precise extant subgenera. Once again, this discovery attests to a considerable degree of diversity in the Dominican amber-producing forests.

The Cretaceous Scorpion Genus, Archaeobuthus, Revisited (Scorpiones: Archaeobuthidae), Chris Baptista, Jorge A. Santiago-Blay, Victor Fet, Michael E. Soleglad

Euscorpius

No abstract provided.

The Scorpions Of Hainan Island, China (Arachnida: Scorpiones), Zhi-Yong Di, Zhi-Jian Cao, Ying-Liang Wu, Lin Zhu, Hui Liu, Wen-Xin Li

Euscorpius

The redescriptions and illustrations of three species, Isometrus (Isometrus) maculatus (DeGeer, 1778), Lychas mucronatus (Fabricius, 1798) (Buthidae), and Liocheles australasiae (Fabricius, 1775) (Hemiscorpiidae) from Hainan Island, China are presented. Distribution data and updated key of Hainan scorpions are provided.

Review Of The Subgenus Pandinurus Fet, 1997 With Descriptions Of Three New Species (Scorpiones, Scorpionidae, Pandinus)., František Kovařík

Euscorpius

Pandinus (Pandinurus) awashensis sp. n. from Ethiopia, P. (P.) somalilandus sp. n. from Somaliland, and P. (P.) lowei sp. n. from Democratic Republic of Congo are described and compared with other species of the subgenus. P. (P.) sudanicus Hirst, 1911 stat. n. previously considered a synonym of P. (P.) magrettii Borelli, 1901 is recognized as a valid species. New data on taxonomic characters and distribution of the subgenus Pandinurus are presented. Also presented are a map of distribution, photos of the localities and a key to species using hitherto unpublished characters of the tarsomere spination formula and sexual …

Effectiveness Of Manual Palpation In The Northern Water Snake, Nerodia Sipedon Sipedon, As A Method To Extract Gut Contents For Dietary Studies, Marcella Ann Cruz

Theses, Dissertations and Capstones

The Common Water Snake, Nerodia sipedon sipedon, is one of six subspecies, and the only species of Nerodia to inhabit West Virginia. Because it is abundant and information on the species is lacking within the state, it was used as my study organism. The effectiveness of manual palpation, the most commonly used method in snake diet related studies, was tested against museum dissection by comparing presence and absence of gut contents. Nineteen individuals of 76 manually palpated collected snakes were compared to 6 individuals of 37 dissected snakes using a Pearson chi-square analysis. Results suggested manual palpation is an effective …

Examining Habitat Selection And Home Range Behavior At Multiple Scales In A Population Of Eastern Box Turtles, (Terrapene C. Carolina), With Notes On Demographic Changes After 17 Years, Brian Anthony Williamson

Theses, Dissertations and Capstones

The Eastern Box Turtle, Terrapene c. carolina, is a terrestrial species native to the eastern United States. Once considered common, it is currently declining in much of its range due to habitat destruction and disease. To conserve the species, knowledge of Eastern Box Turtle habitat selection and the factors influencing their movement is necessary. Although the home range behavior of the Eastern Box Turtle has been well studied, few studies have quantified habitat selection at the home-range scale. Therefore, I examined home-range selection and within home range habitat selection in a population of Eastern Box Turtles at Hungry Beech Nature …

Filling In The Gaps In Phenology And Life History Of The Cumberland Plateau Salamander (Plethodon Kentucki), Robert C. Bowers

Theses, Dissertations and Capstones

The Cumberland Plateau Salamander, Plethodon kentucki, is a member of the Plethodon glutinosus complex comprising 16 sibling species, which are best differentiated by range. Few studies have been conducted to gain information on the natural history of P. kentucki. To alleviate this, two sites at Beech Fork State Park in Wayne County, West Virginia were used to study the salamander’s general life history with emphasis on reproduction, phenology, and population size. At each site, three 20m x 20m sample plots were arranged based on viability of the habitat for P. kentucki. Ground searches of all cover objects …

Gene Transfer In A Newly Established Spontaneous Feline Fibrosarcoma Cell Line, Rounak Nande

Theses, Dissertations and Capstones

Fibrosarcoma is a deadly disease in cats and is most often located at classical vaccine injections sites. More rare forms of spontaneous non-vaccination site (NSV) fibrosarcomas have been described and have been found associated to genetic alterations.

Purpose of this study was to compare the efficacy of adenoviral gene therapy in NVS fibrosarcoma. The NVS fibrosarcoma cell line (Cocca-6A) was isolated from a spontaneous fibrosarcoma that occurred in a domestic calico cat. Cocca-6A fibrosarcoma cells were morphologically and cytogenetically characterized. The feline cells were karyotyped and their chromosome number was counted using a Giemsa staining. Giemsa block staining of metaphase …

Examining The Influence Of Mating Systems On Testes Size In Salamanders, Howard James Stanton Ii

Theses, Dissertations and Capstones

Sperm competition theory predicts that relatively larger testes sizes evolve in animals with polygamous mating systems compared to those in monogamous mating systems due to sperm competition. Whereas intensity of sperm competition is the significant predictor of testes sizes in other taxa such as mammals, frogs, birds, insects, and fish, in salamanders the intensity of male-male competition in the transfer of spermatophores to females is predicted to be a critical factor. This is because males have to deposit more spermatophores to secure reproductive pay-off under higher intensity of male-male competition. I hypothesized that salamander species that breed explosively as groups …

Quantitative Reconstruction And Two-Dimensional, Steady Flow Hydrodynamics Of The Plesiosaur Flipper, Mark Cruz Deblois

Theses, Dissertations and Capstones

Plesiosaurs are a group of extinct marine reptiles that thrived during the Mesozoic Era. They are unique for swimming with two hydrofoil-shaped flippers. Penguins, sea turtles, and cetaceans all have hydrofoil shaped flippers but penguins and sea turtles only use the front pair to produce thrust and cetaceans use their tail flukes. Consequently, the mode of swimming for plesiosaurs has long been debated. However, a quantitative study of the hydrodynamic properties of the flippers, which would constrain inference about their mode of swimming, has not yet been done. The main reason is that the trailing edge of the plesiosaur flipper …

Ontogenetic State Of A Juvenile Polycotylid Plesiosaur (Sauropterygia: Plesiosauria) And Its Implications For Plesiosaur Growth, Christina Joanne Byrd

Theses, Dissertations and Capstones

Understanding ontogenetic variation in plesiosaurs from the family Polycotylidae can provide insight into the evolutionary history of this group of extinct short-necked marine reptiles from the Cretaceous period. In this study, I analyzed a juvenile polycotylid plesiosaur from the University of Nebraska State Museum (UNSM 55810). UNSM 55810 came from the Pierre Shale (99-65 Ma) of Nebraska and possesses skeletal elements that include a partial skull, nearly complete pectoral and pelvic girdles, and an assortment of paddle elements. Based on several cranial characteristics, this specimen is probably referable to the genus Dolichorhynchops. Previous qualitative studies have included UNSM 55810 but …