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Articles 121 - 150 of 296
Full-Text Articles in Animal Sciences
The Role Of Histone Methyltransferases In Determining Developmental Potential Of Bovine Oocytes, Jairo Alberto Sarmiento
The Role Of Histone Methyltransferases In Determining Developmental Potential Of Bovine Oocytes, Jairo Alberto Sarmiento
LSU Doctoral Dissertations
Histone proteins are proteins found in eukaryotic cells that associate with DNA to form the most basic structural unit of chromatin, called nucleosomes. The post-translational modifications of histones regulate developmental competence in bovine oocytes and early embryos. The difference in developmental competence between in vitro matured oocytes and in vivo matured oocytes was used to investigate the accumulation of transcripts for histone methyltransferases (HMTs) during oocyte maturation. Methyltransferases ASH1L, EHMT2, SUV39H1 and KDM6B were selected as genes of interest. Transvaginal ultrasound-guided aspiration (TUGA) was used to collect immature and in vivo matured bovine cumulus-oocyte complexes (COCs). Immature COCs collected via …
Influence Of Feeding Anise Oil To Piglets And Broilers, Jose Wando Charal
Influence Of Feeding Anise Oil To Piglets And Broilers, Jose Wando Charal
LSU Doctoral Dissertations
The objectives of this research were to determine 1) the effect of anise oil (AO) in pigs after exposure and re-exposure by dietary additions to sow and nursery diets; and 2) the maximum level of AO that could be fed to broilers and its effect on in-vitro growth of Clostridium perfringens (ClP); 3) to determine the effect of AO and diet type on growth performance of broilers after a challenge with ClP; and 4) to determine the effect of AO fed to broilers under commercial conditions. The results indicated that exposing lactating pigs to AO through the sow diet had …
Effects Of Dietary Conjugated Linoleic Acid Supplementation On Bovine Oocyte Lipid Metabolism, Lipid Composition And Embryo Cryotolerance, Cody Lee Bailey
Effects Of Dietary Conjugated Linoleic Acid Supplementation On Bovine Oocyte Lipid Metabolism, Lipid Composition And Embryo Cryotolerance, Cody Lee Bailey
LSU Doctoral Dissertations
Variation in cryotolerance exists between embryos from different animal breed, species and management conditions. Reduced tolerance to chilling and cryotolerance of oocytes and embryos has been associated with greater cytoplasmic lipids (Kim et al., 2001; Seidel, 2006). Previous studies in the cow have demonstrated nutrition-induced modification of follicular components. Trans-10, cis-12 conjugated linoleic acid (CLA) was identified as a potent inhibitor of milk fat synthesis in lactating cows (Baumgard et al., 2000) and inclusion of CLA in bovine embryo culture medium improved post-thaw embryo survival (Pereira et al., 2007). Dietary supplementation of cows with CLA could alter oocyte fatty acid …
Response Of Bovine Fetal Fibroblasts To Pluripotency Induction With In Vitro Transcribed Mrna, Tricia Adams
Response Of Bovine Fetal Fibroblasts To Pluripotency Induction With In Vitro Transcribed Mrna, Tricia Adams
LSU Master's Theses
Numerous studies have contributed to the induction of pluripotency in an abundance of cell types; however, transfection techniques and efficiency have yielded undesirable outcomes. Traditionally, the use of viral vectors as a mode of transmission has proven to be efficient in the induction of pluripotency transcription factors in mammalian cells. The increasing concern is random insertion of viral components within the host genome due to the viral mode of replication. The delivery of messenger RNA by cationic lipid delivery vehicles circumvents the viral concerns and provides an efficient and safe mode of reprogramming. Synthetic mRNA can be used to initiate …
The Use Of Duddingtonia Flagrans For Gastrointestinal Parasitic Nematode Control In Feces Of Exotic Artiodactylids At Disney's Animal Kingdom®, Jenna Terry
LSU Master's Theses
Gastrointestinal nematodes (GIN) are parasites of major concern for domestic and exotic ruminant species around the world. In the past, zoological facilities used anthelmintics as their primary control method. Challenges in accurate dosing and administration of anthelmintics to exotic hoofstock contributed to the development of resistant nematode populations in zoological settings. The historic dependency on anthelmintics to control GIN populations is no longer an option. Biological alternatives are urgently needed, in both exotic and domestic ruminants, in the war against resistance. One such alternative is the use of the nematophagous fungus, Duddingtonia flagrans. Three independent studies were conducted: A nine …
Changes In Plasma Melanocyte Stimulating Hormone, Acth, Prolactin, Gh, Lh, Fsh, And Thyroid Stimulating Hormone In Response To Injection Of Sulpiride, Thyrotropin Releasing Hormone, Or Vehicle In Insulin Sensitive And Insensitive Mares, Nicole Arana Valencia
LSU Master's Theses
Six insulin sensitive and six insensitive mares were used in a replicated 3 x 3 Latin square design to determine the pituitary hormonal responses (compared to vehicle) to sulpiride and thyrotropin-releasing hormone (TRH), two compounds commonly used to diagnose pituitary pars intermedia dysfunction (PPID) in horses. Mares were classified as insulin sensitive or insensitive by their previous glucose responses to direct injection of human recombinant insulin. Treatment days were February 25 and March 10 and 24, 2012. Treatments were sulpiride (racemic mixture, 0.01 mg/kg BW), TRH (0.002 mg/kg BW), and vehicle (saline, 0.01 mL/kg BW) administered intravenously. Blood samples were …
Factors Affecting Basal And Post-Exercise Prolactin Secretion In Horses, Lisa C. Digiovanni
Factors Affecting Basal And Post-Exercise Prolactin Secretion In Horses, Lisa C. Digiovanni
LSU Master's Theses
There has been thorough documentation to support the role of dopamine in the control of prolactin production and secretion in various mammalian species, including the horse. However, there is evidence that other factors are involved in prolactin secretion. Seven experiments were conducted to assess factors that potentially might affect prolactin secretion in the horse. The first two experiments were conducted (separately) to test whether arginine vasopressin (AVP) or vasoactive intestinal polypeptide (VIP) affected prolactin secretion. In each experiment, AVP or VIP was administered intravenously and blood samples were collected to determine the effect on prolactin secretion. Neither peptide produced any …
Influence Of "Added" Lactose On Probiotic Properties Of Yogurt Culture Bacteria And Yogurt Characteristics, Behannis Jasmin Mena-Chalas
Influence Of "Added" Lactose On Probiotic Properties Of Yogurt Culture Bacteria And Yogurt Characteristics, Behannis Jasmin Mena-Chalas
LSU Master's Theses
Lactose sugar is a source of energy for Lactic Acid Bacteria (LAB) in dairy fermented products. Enrichment of yogurt with lactose addition may increase growth and viability of the yogurt starter culture (Streptococcus thermophilus and Lactobacillus delbrueckii ssp. bulgaricus) and enhance yogurt physico-chemical and sensory attributes. The objectives of this study were: to determine the influence of added lactose on (1) acid and bile tolerance of yogurt starter culture Streptococcus thermophilus ST-M5 and Lactobacillus bulgaricus LB-12, (2) the final lactose content of yogurt during its shelf life, (3) the physico-chemical characteristics of yogurt during shelf life, (4) the growth of …
Effects Of Different Weaning Management Strategies On Preconditioning Performance, Haptoglobin Serum Levels, Feedlot Morbidity, And Carcass Characteristics, Jake Everitt Anderson
Effects Of Different Weaning Management Strategies On Preconditioning Performance, Haptoglobin Serum Levels, Feedlot Morbidity, And Carcass Characteristics, Jake Everitt Anderson
LSU Master's Theses
Weaning, one of the first major stressors encountered by the calf, has a negative effect on the immune system and increases the likelihood of infection of novel pathogens such as those that cause bovine respiratory disease. Fenceline contact at weaning has been shown to reduce the stress on the calf during the time following maternal separation. Preconditioning programs have been shown to reduce feedlot morbidity and mortality. Combining these two management practices could reduce the length of time calves need to be held in a preconditioning program. A multi-year study was conducted to evaluate if fenceline weaning will allow for …
Yogurt Cultured Milk Powder As A Substitute For Yogurt Powder, Lijie Song
Yogurt Cultured Milk Powder As A Substitute For Yogurt Powder, Lijie Song
LSU Master's Theses
Commercial yogurt powder has to go through drying process which kills the yogurt culture, so the health benefit of the yogurt culture bacteria are lost. Also, upon reconstitution commercial yogurt powder does not taste like yogurt, it is sour and off flavored. The hypothesis of this study was that yogurt cultured milk powder would have better culture bacterial counts, better physico-chemical and sensory characteristics than commercial yogurt powder currently available. Commercial yogurt powder (CYP) was the control and yogurt cultured milk powder (YCMP) was the treatment. Freeze-dried yogurt starter culture (Lactobacillus bulgaricus and Streptococcus thermophilus at ratio 1:1) was added …
Evaluation Of 54 Years Of Louisiana Bull Testing, And Snp Affecting Growth And Performance Of Yearling Bulls On A Forage Performance Bull Test, Tabitha Howard
Evaluation Of 54 Years Of Louisiana Bull Testing, And Snp Affecting Growth And Performance Of Yearling Bulls On A Forage Performance Bull Test, Tabitha Howard
LSU Master's Theses
The first objective of these two studies was to evaluate genetic trends for bulls that have comprised the LSU AgCenter Dean Lee performance bull test for the past 55 years. Data included birth weight (BW), initial weight, 112-day weight, average daily gain (ADG), adjusted yearling weight, and scrotal circumference (SC), on 7,488 yearling bulls of 34 breeds on 112d of test for the last 55 years. The top 4 represented bull breeds with greater than 500 animals (Angus, Charolais, Hereford, and Simmental) were included in this analysis. Analyses revealed that growth traits for all bulls regardless of breed demonstrated a …
Influence Of "Added" Whey Protein Isolate On Probiotic Properties Of Yogurt Culture Bacteria And Yogurt Characteristics, Luis Alfonso Vargas Lopez
Influence Of "Added" Whey Protein Isolate On Probiotic Properties Of Yogurt Culture Bacteria And Yogurt Characteristics, Luis Alfonso Vargas Lopez
LSU Master's Theses
Consumers are becoming conscious of their diet, increasing protein intake and avoiding carbohydrates and fats. Whey proteins have branch chain amino acids responsible for muscle building. Whey protein isolate (WPI) contains more than 90% protein. The effect of incremental addition of WPI on probiotic characteristics of pure cultures and cultures in yogurt and yogurt characteristics are not known. The hypothesis was that “added” WPI will influence the characteristics of yogurt culture bacteria in pure form and in yogurt. The objectives were: to determine the influence of added WPI on (1) acid and bile tolerance, growth and protease activity of pure …
A Genomic And Quantitative Evaluation Of Modern Charolais Sired Calves Versus Multigenerational Angus Sired Calves For Growth And Carcass Quality And Compostion Traits, Jennifer Lynn Bailey
A Genomic And Quantitative Evaluation Of Modern Charolais Sired Calves Versus Multigenerational Angus Sired Calves For Growth And Carcass Quality And Compostion Traits, Jennifer Lynn Bailey
LSU Master's Theses
The objective of this study is to evaluate the association of single nucleotide polymorphisms with growth, performance, and carcass quality and composition characteristics in a population of cattle consisting of multigenerational Angus sired calves and modern characteristic Charolais sired calves. A total of 132 calves were evaluated. Due to a limited sample population statistical difference was set at p ˂ 0.05 and statistical trends were set at p ˂ 0.1. Mean birth weights were significantly higher for Charolais sired calves as compared to Angus sired calves. Significant differences were observed between the two breed types for mean weaning weights with …
Vitrification Of Equine Expanded Blastocysts, Fabian Andres Diaz
Vitrification Of Equine Expanded Blastocysts, Fabian Andres Diaz
LSU Master's Theses
The cryopreservation of equine expanded blastocysts (>300 um) has been largely unsuccessful primarily due to the low permeability to cryoprotectants and the large size of the equine embryo. Mechanical alternatives may provide means to overcome the capsule barrier and the relative large embryo size. In this regard, multiple experiments were performed in this study to evaluate different approaches of capsule puncture and blastocoele fluid extraction with the objective to develop a cryopreservation protocol for Day 8 equine expanded blastocysts. In the first experiment, twenty-four Day 8 expanded blastocysts were exposed to standard equine embryo vitrification solutions following one- or …
Effects Of Meal Timing On Growth Hormones, Ghrelin, And Insulin Sensitivity In Male Holstein Calves, Erica Lynn Chartier
Effects Of Meal Timing On Growth Hormones, Ghrelin, And Insulin Sensitivity In Male Holstein Calves, Erica Lynn Chartier
LSU Master's Theses
Eighteen neonatal Holstein bull calves (38.85 ± 4.71 kg) were assigned to one of two treatments at birth to determine the effect of feeding time on growth, nutrient intake, metabolic hormone secretion, and energy metabolism. Regularly fed calves (n = 9) were fed MR daily at 0630 h, and irregularly fed calves (n = 9) at 1030, 0800, 0630, 0830, 0530, 0930, and 0730 Monday through Sunday. Body weights were measured weekly from birth to 9 weeks. Water intake, fecal scores, and starter intake were measured daily. Serial blood collections were conducted at 2, 4, 6, and 8 weeks for …
Influence Of Various Health Beneficial Spices On Some Characteristics Of Yogurt Culture Bacteria Lactobacillus Acidophilus, And Sensor Acceptability Of Spicy Probiotic Yogurt, Margie Michelle Sanchez-Vega
Influence Of Various Health Beneficial Spices On Some Characteristics Of Yogurt Culture Bacteria Lactobacillus Acidophilus, And Sensor Acceptability Of Spicy Probiotic Yogurt, Margie Michelle Sanchez-Vega
LSU Doctoral Dissertations
There is a pronounced public awareness about herbal remedies. Garlic and ginger have antibacterial properties and prevent cardiovascular diseases. Onion and turmeric decrease the risk of diabetes and like garlic, they have anticancer properties. Streptococcus thermophilus, Lactobacillus bulgaricus and Lactobacillus acidophilus are lactic acid bacteria that produce lactase and reduce the symptoms of malabsorption. Earlier work has shown the influence of spice extracts but the influence of pure spice juice on yogurt culture bacteria is not known. Characteristics of yogurt culture bacteria were measured by suspending freshly thawed cultures in 0.1% peptone water (growth), acidified MRS broth (acid tolerance), MRS-Thio …
Reduced Sodium Delivery Through Submicronization Of Sodium Chloride, Its Use In The Manufacture Of Surface Salted Cheese Crackers And The Evaluation Of Physico-Chemical, Microbiological And Sensory Characteristics Of Cheese Crackers, Marvin L. Moncada-Reyes
LSU Doctoral Dissertations
Reducing the particle size of sodium chloride crystal would increase its dissolution rate leading to a more efficient transfer of the ions to the taste buds and hence perhaps a saltier perception of foods. The objective of this study was to develop submicrosalt by using a nanospray drying method, its use in surface salted cheese crackers and evaluating their physico-chemical, microbiological and sensory characteristics. The cheese cracker treatments consisted of 3 different salt sizes (regular, microsalt and submicrosalt) and 3 different concentrations (2, 1.5 and 1%). The 9 (3 sizes x 3 concentrations) different cheese cracker treatments were tested for …
Evaluating The Performance Of F1 Brahman Sired Calves Compared To F1 Angus Sired Calves, Shari Alicia Mahoney
Evaluating The Performance Of F1 Brahman Sired Calves Compared To F1 Angus Sired Calves, Shari Alicia Mahoney
Honors Capstones
No abstract provided.
Repeatability Of Prolactin Responses To Sulpiride In Mares And Geldings And The Effect Of Pergolide And Cabergoline, Rebekah C. Hebert
Repeatability Of Prolactin Responses To Sulpiride In Mares And Geldings And The Effect Of Pergolide And Cabergoline, Rebekah C. Hebert
LSU Master's Theses
Four experiments were conducted in an effort to develop a method, based on prolactin secretion, for assessing the efficacy and duration of activity of dopaminergic agonists for the treatment of pituitary pars intermedia dysfunction (PPID) in horses. In the first experiment, prolactin response to a low dose of the dopamine antagonist, sulpiride, was generally repeatable in estrogen-primed geldings in winter over 8 every-other-day challenges. It was concluded that estrogen-primed, sulpiride-challenged geldings in winter could serve as a model for the study of potential dopaminergic drugs for the treatment of PPID in horses. The second experiment was performed in the summer …
Lentiviral Transduction Of Epigenetically Modified Bovine Adult Stem Cells, Meredith Kathleen Addison
Lentiviral Transduction Of Epigenetically Modified Bovine Adult Stem Cells, Meredith Kathleen Addison
LSU Master's Theses
Bovine adipose-derived stem cells (ADS), a form of adult stem cells, are somatic cells that have similar characteristics of embryonic stem (ES) cells. Bovine ADS cells possess multipotent capabilities and have been found to express pluripotency genes associated with ES cells. The unique properties of ADS cells make them a desirable source for reprogramming experiments. The goal of reprogramming experiments is to transform somatic cells from a differentiated state to a pluripotent state. When somatic cells reprogram, there are certain epigenetic changes or modifications that must occur in order to successfully reprogram the nucleus. Epigenetic modifications will change the chromatin …
Evaluation Of Single Nucleotide Polymorphisms Associated With Fertility And Production Traits In Holstein And Multi-Generational Angus Females, Rebecca Ann Hill
Evaluation Of Single Nucleotide Polymorphisms Associated With Fertility And Production Traits In Holstein And Multi-Generational Angus Females, Rebecca Ann Hill
LSU Master's Theses
The objective of this study was to test the association of single nucleotide polymorphisms (SNPs) with fertility in two populations consisting of Holstein cows and multi-generational Angus cows. The candidate gene approach was utilized and previously described SNPs were tested for possible associations with fertility. Single nucleotide polymorphisms on three genes were evaluated including leptin receptor LEPR, calpastatin CAST, and DGAT1. Fertility traits were evaluated in conjunction with production traits for Holstein females and growth traits for Angus females. One SNP was significantly associated with birth weight (P < 0.05) in Angus females while a trend (P < 0.10) was observed for two markers influencing birth weight performance and three markers influencing weaning weight performance. An association of two SNP for birth weight and back fat thickness in Angus females was identified.!A trend (P < 0.10) was observed for one marker within LEPR influencing average services to conception, two markers within CAST influencing average days open, two markers within CAST and one marker within DGAT1 influencing average protein production, and one marker within CAST and one marker within DGAT1 influencing average milk production. One SNP within LEPR was significantly associated with average milk production (P < 0.05) in Holstein females. An association of one SNP within CAST and one SNP within DGAT1 for average protein production and average milk production in Holstein females was identified. An association of one SNP within CAST for average days open and average protein production in Holstein females was also identified. The association of these markers indicates that the evaluated quantitative trait loci (QTL) region may harbor causative mutations responsible for the variation observed in fertility and production traits. Further evaluation of SNP in these regions is necessary in order to identify mutations accounting for the largest degree of variation for fertility and production traits.
Evaluation Of Methods For Suppressing Estrus And Ovulation In Mares: Sustained Release Injections Of Altrenogest Versus Deslorelin Acetate, Thomas J. Stevens
Evaluation Of Methods For Suppressing Estrus And Ovulation In Mares: Sustained Release Injections Of Altrenogest Versus Deslorelin Acetate, Thomas J. Stevens
LSU Master's Theses
Two experiments assessed the potential of altrenogest, an artificial progestogen, and deslorelin acetate, a gonadotropin-releasing hormone (GnRH) agonist, for suppressing estrus and ovulation in cyclic mares. In the first experiment, mares were administered a luteolytic dose of prostaglandin-F2á on d 6 of diestrus, and were then treated with 1 of 3 formulations of altrenogest in slow-release vehicle (6 mares/group): 1) Biorelease altrenogest LA150 (225 mg total as a 1.5 mL injection); 2) Biorelease altrenogest LA225 (225 mg total as a 1.0 mL injection); or 3) Biorelease altrenogest LA225 (450 mg total as a 2.0 mL injection). Six control mares received …
Forage Systems For Finishing Steers In South Louisiana, Jose M. Rodriguez
Forage Systems For Finishing Steers In South Louisiana, Jose M. Rodriguez
LSU Master's Theses
Research has found conflicting results on animal performance and carcass traits associated with the use of forage as the primary feed source for finishing cattle. Consumer interest in forage-fed products has grown and little research has been done comparing performance of forage-fed animals and beef finished on different forages. Spring weaned calves (n=54; 257 ± 2.5 kg; 3/8 Gelbvieh, 3/8 Red Angus, and 1/4 Brahman) were used in the evaluation of three forage systems (S1, S2, and S3) on a 100% forage diet in two consecutive years (June 2009 and 2010). Steers were divided into 9 groups based on initial …
The Effect Of Frozen Storage On The Survival Of Probiotic Microorganisms Found In Traditional And Commercial Kefir, Keely Virginia O'Brien
The Effect Of Frozen Storage On The Survival Of Probiotic Microorganisms Found In Traditional And Commercial Kefir, Keely Virginia O'Brien
LSU Master's Theses
Kefir is a fermented milk traditionally made from a unique starter culture, which consists of numerous bacteria and yeast species bound together in an exopolysaccharide matrix produced by certain lactic acid bacteria. Many health benefits are associated with traditionally produced kefir; however, bulging and leaking packaging, caused by secondary yeast fermentation during storage, has limited large scale manufacture traditionally produced kefir. Commercial kefir products have been designed to reduce these effects by using a pure starter culture consisting of a mixture of bacteria and yeast species that give a flavor similar to traditional kefir, but some health benefits may be …
Reduced Protein For Late-Lactation Dairy Cows, Andre De Barros Duarte Pereira
Reduced Protein For Late-Lactation Dairy Cows, Andre De Barros Duarte Pereira
LSU Master's Theses
Excess protein in dairy cattle diets unnecessarily increases the cost of production and may contribute to environmental pollution. The objective of this research was to evaluate the effect of feeding dairy cows with two levels of dietary protein on animal performance and manure characteristics. Two experiments were carried out with 24 lactating dairy cows each. Experiment 1 was corn silage based and had a control TMR (HP1) estimated to contain 16.5% CP with SBM and treatment TMR (LP1; 13.5% CP) using DDGS and rumen protected Lys and Met. Experiment 2 was ryegrass haylage based and had a control TMR (HP2) …
Yogurt Cultures Survive Upon Exposure To Two Antimicrobials And Streptococcus Thermophilus St-M5 Acquired Resistance To Both Antimicrobials, Maria Carolina Vives Habeych
Yogurt Cultures Survive Upon Exposure To Two Antimicrobials And Streptococcus Thermophilus St-M5 Acquired Resistance To Both Antimicrobials, Maria Carolina Vives Habeych
LSU Master's Theses
Different antimicrobials are added in the manufacturing of dairy products such as flavored yogurts and processed cheese. Potassium Metabisulfite (PM) and Potassium Nitrite (PN) have been reported to have antimicrobial effect on pathogenic microorganisms such as Clostridium botulinum and Listeria monocytogenes. Yogurt is known for its health benefits, due to the presence of cultured bacteria. PM and PN are not commonly used in the dairy industry hence it would be interesting to study their influence in yogurt culture bacteria. The objectives of this study were: 1. to elucidate the influence of PM and PN at various concentrations, on the growth …
Insulin Dose Response Curves And Factors Affecting Insulin Sensitivity In Horses, Jeanne D. Lestelle
Insulin Dose Response Curves And Factors Affecting Insulin Sensitivity In Horses, Jeanne D. Lestelle
LSU Master's Theses
Three experiments were conducted to further study the use of intravenous insulin injection to measure insulin sensitivity in horses. In the first experiment, glucose responses to multiple doses of recombinant human insulin were compared in insulin sensitive and insensitive mares. Regression lines describing the responses in insensitive mares had less (P = 0.003) steep slopes and greater (P = 0.006) effective doses at 50% response (ED50) compared to sensitive mares. Curvilinear regression models provided a good fit (R2 = 0.95) for the prediction of ED50 from the individual responses to 50 mU/kg BW insulin. The second experiment estimated the repeatability …
A Novel Immunomodulatory Subunit Vaccine To Combat The Involvement Of Bovine Respiratory Coronavirus Infections In Shipping Fever, Genevieve Elizabeth Lum
A Novel Immunomodulatory Subunit Vaccine To Combat The Involvement Of Bovine Respiratory Coronavirus Infections In Shipping Fever, Genevieve Elizabeth Lum
LSU Doctoral Dissertations
Bovine respiratory coronavirus (BRCoV) is a group 2a coronavirus expressing both hemagglutinin-esterase and spike (S) envelope glycoproteins. The S glycoprotein is a primary coronavirus virulence factor responsible for both receptor specificity and membrane fusion-mediated entry into host cells. In addition, the S glycoprotein serves as a major antigen targeted by both the cellular and humoral immune responses and, as such, is an important target for antibody-facilitated virus neutralization. The objective of this research was the design of a safe and effective vaccine against BRCoV using a “prime-boost” vaccination approach. This method utilized an initial DNA vaccine encoding either the soluble …
Comparison Of Epididymal And Ejaculated Sperm Collected From The Same Holstein Bulls, Michael A. Stout
Comparison Of Epididymal And Ejaculated Sperm Collected From The Same Holstein Bulls, Michael A. Stout
LSU Doctoral Dissertations
Salvaging of epididymal sperm from injured or deceased animals allows for the propagation of favorable traits from endangered or genetically superior males. Techniques developed in domestic species can also serve as the foundation for the collection, cryopreservation and utilization of epididymal sperm in exotic breeds. The need to preserve and utilize epididymal sperm, in the most efficient manner, is of the utmost importance. In a series of experiments, ejaculated and epididymal sperm from the same four mature, fertile, Holstein bulls were collected, cryopreserved, cultured and used for in vitro fertilization. In Experiment I, epididymal sperm was found to have higher …
Development Of An Estradiol-Dopamine Anagonist Protocol For Inducing Ovulation In Seasonally Anovulatory Mares, Pamela Boliew Mitcham
Development Of An Estradiol-Dopamine Anagonist Protocol For Inducing Ovulation In Seasonally Anovulatory Mares, Pamela Boliew Mitcham
LSU Doctoral Dissertations
Five experiments were conducted to assess potential improvements in a protocol for inducing ovulation in seasonally anovulatory mares based upon estradiol pretreatment followed by dopamine antagonist injection. The first experiment compared various doses of estradiol cypionate (ECP) and domperidone (in biodegradable microparticles), as well as additions and deletions to the protocol. It was concluded that as little as 75 mg ECP and as little as 1.5 g of domperidone could be used with success, but that both components were required. In the second experiment, timing of the injection (1, 6, or 11 days apart) and ECP dose were assessed. It …