Animal Sciences Commons^™

Comparisons Of Fish Species Inside And Outside Of Marine Protected Areas Off The South Central Coast Of California, David E. Rasmussen

Master's Theses

COMPARISONS OF FISH SPECIES INSIDE AND OUTSIDE OF MARINE PROTECTED AREAS OFF THE SOUTH CENTRAL COAST OF CALIFORNIA

David E. Rasmussen

From 2004-2008 and 2008-2009 Cal Poly University researchers conducted two separate tag and recapture studies of nearshore fish populations along California’s central coast. Three locations were sampled that have experienced different degrees of fishing pressure. Big Creek MPA has been closed to all fishing from 1993 onwards and is the farthest from port of all locations. Cambria lies closest to port of the three locations and because of this has the highest fishing pressure. Piedras Blancas lies midway between …

Delivering Health And Wellness, Phillip S. Tong

Dairy Science

No abstract provided.

The Impact Of Antioxidant Addition On Flavor Of Cheddar And Mozzarella Whey And Cheddar Whey Protein Concentrate, I. W. Liaw, H. Eshpari, P. S. Tong, M. A. Drake

Dairy Science

Lipid oxidation products are primary contributors to whey ingredient off-flavors. The objectives of this study were to evaluate the impact of antioxidant addition in prevention of flavor deterioration of fluid whey and spray-dried whey protein. Cheddar and Mozzarella cheeses were manufactured in triplicate. Fresh whey was collected, pasteurized, and defatted by centrifugal separation. Subsequently, 0.05% (w/w) ascorbic acid or 0.5% (w/w) whey protein hydrolysate (WPH) were added to the pasteurized whey. A control with no antioxidant addition was also evaluated. Wheys were stored at 3 °C and evaluated after 0, 2, 4, 6, and 8 d. In a subsequent experiment, …

Effect Of Feeding Zilpaterol Hydrochloride For 20 Days To Calf-Fed Holstein Steers With A 3 Or 10 Day Withdrawal Period Antemortem On Carcass Characteristics And Tenderness, Andrew D. Hosford

Master's Theses

The effect of feeding Zilpaterol hydrochloride (ZH) with a 3 or 10 d withdrawal (WD) period to calf-fed Holstein steers (N=2993) on carcass characteristics and tenderness were evaluated in a feed lot experiment. Cattle were fed 0 or 8.3 mg/kg of ZH for the final 20 d of the feeding period, each treatment level was assigned a WD period of either 3 or 10 d. Treatment groups consisted of Control 3 d WD (C3) and 10 d WD (C10), and ZH fed 3 day WD (Z3) and 10 d WD (Z10). Cattle were slaughtered at a commercial facility, carcasses chilled …

Effects On Lactation Performance When Slick Hair Gene Is Simulated In Dairy Cattle In The Tropics, R. M. Mejía, J. A. Ortuño, G. J. Lascano, M. Vélez

Dairy Science

No abstract provided.

Digestibility Of Fresh Sugarcane-Based Diets With Slow-Release Non Protein Nitrogen Addition For Limit-Fed Dairy Heifers In The Tropics, G. J. Lascano, M. Velez, J. M. Tricarico, A. J. Heinrichs

Dairy Science

No abstract provided.

Dietary Starch Level And Dose Response Of Saccharomyces Cerevisiae For Limit Fed-Dairy Heifers, G. J. Lascano, J. M. Tricarico, A. J. Heinrichs

Dairy Science

No abstract provided.

Nutrient Utilization Of Different Levels Of Dietary Fiber In Dairy Heifers Limit-Fed High And Low Concentrate Diets, G. J. Lascano, A. J. Heinrichs

Dairy Science

No abstract provided.

Trial And Budget Analysis Of Two Synchronized Artificial Insemination Breeding Programs For Beef First Calf Heifers, John Nino

Agribusiness

This study was designed to compare the benefits and costs of two different synchronized artificial insemination programs on two groups of beef first calf heifers. A financial budget was developed that would include cost of synchronized insemination per animal and the added value of a bred heifer compared to an open one.

The two synchronized programs were called controlled internal drug release (CIDR) and Ovsynch. They used a specific timeline and drugs to complete the synchronization process. A single low birth weight high calving ease bull was used for both groups to enforce conformity between the groups. Costs of all …

Design, Planning, And Evaluation Of Upgraded Cattle Handling Facility, Lindsey Kaeli Tulloch

BioResource and Agricultural Engineering

This senior project discusses the design, planning, and evaluation of a livestock handling facility for Tulloch Ranches. The facility will be an upgrade to the facility currently used at the ranch in Pine Valley, California. The design parameters for the facility were narrowed down to the following: 1. Must reduce stress to animals during handling. 2. Must be cost effective to save labor and maintenance. 3. Must show improvement from the current set- up. The above parameters combine to form a system that enables the handlers of Tulloch Ranches to manage and work cattle in a more calm and humane …

Poisonous Rangeland Plants In San Luis Obispo County, Sara Litten, Amanda Ou

Animal Science

Poisonous Rangeland Plants in San Luis Obispo County is a comprehensive educational guide to rangeland plants that are toxic to domestic livestock. This guide begins with an exploration of how the biological systems are affected by the poisonous plant toxins. The biochemistry behind these toxins is included in the discussion. Next, reference material for fourteen plants that inhabit San Luis Obispo County is provided. This information includes specific toxins found in poisonous plants, affected animals, symptoms of poisoning, stages of growth, lethal dose, and distribution of the plant in California. This section of the guide is filled with helpful photos …

Growth Hormone Alters Components Related To Differentiation, Metabolism And Milk Synthesis And Secretion In Mac-T Cells, Tasha Lynn Johnson

Master's Theses

The mammary alveolar cell-T (MAC-T) cell line is able to uniformly differentiate and secrete casein proteins in response to dexamethasone, insulin and prolactin and is extensively used to study bovine mammary epithelial cell function. Growth hormone (GH) has been shown to increase milk protein synthesis both in vivo and in mammary cell models, and induce cytoskeletal rearrangement in 3T3 fibroblast cell line and a Chinese hamster ovary (CHO) cell line. Few studies have focused on identifying the mechanisms involved in differentiated MAC-T cells’ response to GH. We tested the hypothesis that MAC-T cells would respond directly to GH and that …

Growth Hormone Alters Lipid Composition And Increases The Abundance Of Casein And Lactalbumin Mrna In The Mac-T Cell Line, Tasha L. Johnson, Brent A. S. Fujimoto, Rafael Jiménez-Flores, Daniel G. Peterson

Dairy Science

The MAC-T cell line has been used extensively to investigate bovine mammary epithelial cell function. A lactogenic phenotype is generally induced in this cell line by a combination of dexamethasone, insulin and prolactin and has typically been assessed by milk protein production. Few studies have focused on identifying other factors that may affect milk protein synthesis in the MAC-T cell line, and none have considered the lipid class distribution of MAC-T cells as a component of the lactogenic phenotype. Growth hormone (GH) has been shown to increase milk protein synthesis both in vivo and in mammary cell models, and has …

Lipid Rafts In The Bovine Milk Fat Globule Membrane Revealed By The Lateral Segregation Of Phospholipids And Heterogeneous Distribution Of Glycoproteins, Christelle Lopez, Marie-Noëlle Madec, Rafael Jiménez-Flores

Dairy Science

This study reveals the lateral organisation of the milk fat globule membrane (MFGM). Using confocal laser scanning microscopy (CLSM) and a lipid soluble molecule, an exogenous phospholipid and two lectins as fluorescent probes we located triacylglycerols in the core of fat globules and investigated the organisation of the polar lipids and glycoproteins of the MFGM, in situ in milk. Lipid rafts corresponding to the lateral segregation of sphingolipids in liquid-ordered phases surrounded by liquid-disordered domains composed by the glycerophospholipids were observed in the MFGM. These lipid rafts which correspond to rigid sphingolipid-rich domains have a circular shape at room temperature. …

Growth Hormone Alters Lipid Composition And Increases The Abundance Of Casein And Lactalbumin Mrna In The Mac-T Cell Line, Tasha L. Johnson, Brent As. Fujimoto, Rafael Jimenez-Flores, Daniel G. Peterson

Animal Science

The MAC-T cell line has been used extensively to investigate bovine mammary epithelial cell function. A lactogenic phenotype is generally induced in this cell line by a combination of dexamethasone, insulin and prolactin and has typically been assessed by milk protein production. Few studies have focused on identifying other factors that may affect milk protein synthesis in the MAC-T cell line, and none have considered the lipid class distribution of MAC-T cells as a component of the lactogenic phenotype. Growth hormone (GH) has been shown to increase milk protein synthesis both in vivo and in mammary cell models, and has …

Culturally Speaking: Beyond Yogurt, Phillip S. Tong

Dairy Science

No abstract provided.

Perspectives On The Application Of Zilpaterol Hydrochloride In The United States Beef Industry, R. J. Delmore, J. M. Hodgen, B. J. Johnson

Animal Science

Zilpaterol hydrochloride (ZH) is a β-adrenergic agonist approved to be fed at a rate of 8.3 mg/kg (100% DM basis) during the final 20 to 40 d of the finishing period in beef cattle followed by a minimum 3-d withdrawal period antemortem. The Federal Drug Administration (FDA) approved label claims of increased rate of BW gain, improved feed efficiency, and increased carcass leanness. Before the release of ZH for commercial use in 2007, approximately 10 independent research trials at various universities and commercial feedlots were initiated. Articles in recent issues of the Journal of Animal Science are a result of …

Perspectives On The Application Of Zilpaterol Hydrochloride In The United States Beef Industry, Robert J. Delmore, Jr., J. M. Hodgen, B. J. Johnson

Animal Science

Zilpaterol hydrochloride (ZH) is a β-adrenergic agonist approved to be fed at a rate of 8.3 mg/kg (100% DM basis) during the final 20 to 40 d of the finishing period in beef cattle followed by a minimum 3-d withdrawal period antemortem. The Federal Drug Administration (FDA) approved label claims of increased rate of BW gain, improved feed efficiency, and increased carcass leanness. Before the release of ZH for commercial use in 2007, approximately 10 independent research trials at various universities and commercial feedlots were initiated. Articles in recent issues of the Journal of Animal Science are a result of …

Mesenchymal Stem Cells Isolated From A Novel Equine Tissue Exhibit Embryonic Molecular Markers And Adipogenic Differentiation Potential, Jane Marie Isquith, Kelly Shaw, Karen Shaw, Daniel Peterson, Matthew Burd

Animal Science

Mesenchymal stem cells (MSC) have been isolated from equine tissues, most notably adipose and bonemarrow. On the basis of ability for self-renewal, molecular marker expression and differentiation potential, we characterized MSCs isolated from a tissue not yet explored in the equine. After isolation, MSCs weremaintained in culture for 25 passages before senescence was observed. Reverse transcription-PCR revealedembryonic transcription factor Oct4 mRNA, as well as mRNA for fucosyltransferase 4 and ST3B-galactosid α 2,3 sialySSEA4, respylxanthine l ltransferase 2, two enzymes responsible for synthesis of embryonic surface markersSSEA1 and ectively. Treatment with insulin, dexamethasone, indomethacin andisobutylmethed to increased oil red O staining …

Lh Increases Insl3 Mrna Abundance In Ma-10 Leydig Cells, Mary Elizabeth Strong, Matthew Anderson Burd, Daniel Gunnar Peterson

Animal Science

Leydig cells produce testosterone in response to luteinizing hormone (LH) via the cyclic adenosine monophosphate (cAMP)/protein kinase A pathway. Additionally, these cells are responsible for producing insulin-like peptide 3 (INSL3), a peptide hormone that is essential for testicular descent. The insl3 promoterin Leydig cells can be activated by cAMP through the transcription factor Nur77. While the mechanism of LH action on testosterone production is well characterized, the effect of LH on insl3 abundance has yet to be shown directly. MA-10 Leydig cells were treated with either 0 and 100 ng/mL LH for 3 and 6 hours in a 2x2 factorial …

Comparison Of The Effects Of Growth Hormone On Mac-T Cells And Primary Mammary Cells, Lisa Mcdonnell, Tasha Johnson, Daniel G. Peterson

Animal Science

The ability of growth hormone (GH) to increase milk yield through somatomedins is well characterized,however recent studies utilizing mammary epithelial cell (MEC) lines have indicated a possible direct effecton MEC. In the mammary alveolar cell-T (MAC-T) line, relative abundance of mRNA for two milk protein genes, α-lactalbumin and αS1-casein, and GH receptor was increased by GH. MAC-T cells were compared tobovine mammary tissue explants and primary MEC isolated from raw milk through culture in classicallactation medium (dexamethasone, insulin, prolactin) with 0 or 10 ng/mL GH. Explant and primary cellmRNA abundance showed a similar pattern to MAC-T indicating that the effects …

Identifying Alternative Waste Conversion Technologies For Use At California Polytechnic State University San Luis Obispo, Matthew R. Bono

BioResource and Agricultural Engineering

This project was undertaken to identify and study alternative technologies for converting waste streams available on the Cal Poly San Luis Obispo campus into energy. As a large campus on the Central Coast of California Cal Poly not only produces lots of waste, but has limited options for its disposal. Furthermore, the campus is in the midst of an on-going green initiative, and more efficient use of its waste products would be a welcome development for the entire campus community. This report gathers information on waste streams originating at Cal Poly and information about waste to energy conversion technologies. The …

Culturally Speaking: The Year Of Sodium, Phillip S. Tong

Dairy Science

No abstract provided.

The Presence Of Micropterus Salmoides (Largemouth Bass) Influences The Populations Of Rana Draytonii (California Red-Legged Frog) And Pseudacris Regilla (Pacific Treefrog) In Two Ponds In Santa Barbara County, California, Kenneth Lee Gilliland

Master's Theses

Alien fish have been implicated in the decline of Rana draytonii (California red-legged frog) and Pseudacris regilla (Pacific treefrog) populations. Micropterus salmoides (largemouth bass) is a common sport fish that has been introduced into the sympatric range of these two anuran species; however, no studies have investigated the individual influence that this alien fish has on these two species. I conducted M. salmoides control or eradication experiments during a four year study in two ponds within the Transverse Mountain Range of Santa Barbara County, California. Changes in the densities of all life stages of R. draytonii and P. regilla were …

The Effects Of Zilpaterol Hydrochloride On Carcass Cutability And Tenderness Of Calf-Fed Holstein Steers, A. J. Garmyn, J. N. Shook, D. L. Vanoverbeke, J. L. Beckett, Robert J. Delmore, D. A. Yates, D. M. Allen, G. G. Hilton

Animal Science

To evaluate the impact of zilpaterol hydrochloride (ZH) on carcass cutability and tenderness of calf-fed Holstein steers, calf-fed Holstein carcasses (n = 102) were selected from a pool of 2,300 steers that were fed 0 or 8.3 mg/kg (DM basis) of ZH. Zilpaterol hydrochloride was supplemented the last 20 d of the finishing period and withdrawn for 3 d before slaughter. Carcasses were selected based on carcass weight as well as predetermined USDA Yield grade categories. For tenderness evaluation, steaks from the strip loin, bottom round, and top round (n = 54 per subprimal) were aged for 14 or 21 …

Effects Of Zilpaterol Hydrochloride Feeding Duration On Beed And Calf-Fed Holstein Strip Loin Steak Color, H. R. Rogers, J. C. Brooks, M. C. Hunt, G. G. Hilton, D. L. Vanoverbeke, J. Killefer, T. E. Lawrence, R. J. Delmore, B. J. Johnson, D. M. Allen, M. N. Streeter, W. T. Nichols, J. P. Hutcheson, D. A. Yates, J. N. Martin, M. F. Miller

Animal Science

ABSTRACT: Two studies using beef and calf-fed Holstein cattle were conducted to determine the effect of zilpaterol hydrochloride (ZH) supplementation on the color of strip loin steaks packaged in traditional and modified-atmosphere packaging. Select (USDA) strip loins were obtained from the carcasses of beef (n = 118) or calf-fed Holstein (n = 132) cattle fed ZH (6.8 g/ton on a 90% DM basis) for the last 0, 20, 30, or 40 d of feeding. One portion of the strip loin was moisture enhanced, cut into steaks, and packaged in an atmosphere containing 80% oxygen and 20% carbon dioxide. The remaining …

Investigating The Protective Properties Of Milk Phospholipids Against Ultraviolet Light Exposure In A Skin Equivalent Model, Ashley Russell, Andrea Laubscher, Rafael Jiménez-Flores, Lily H. Laiho

Dairy Science

Current research on bioactive molecules in milk has documented health advantages of bovine milk and its components. Milk Phospholipids, selected for this study, represent molecules with great potential benefit in human health and nutrition. In this study we used confocal reflectance and multiphoton microscopy to monitor changes in skin morphology upon skin exposure to ultraviolet light and evaluate the potential of milk phospholipids in preventing photodamage to skin equivalent models. The results suggest that milk phospholipids act upon skin cells in a protective manner against the effect of ultraviolet (UV) radiation. Similar results were obtained from MTT tissue viability assay …

Culturally Speaking: Ethnic Dairy Foods, Phillip S. Tong

Dairy Science

No abstract provided.

Dairy Cow Grouping, Allison Mcmillan

Dairy Science

No abstract provided.

Relationship Of Copper, Zinc And Selenium Status With Udder Health And Mastitis Incidence In The Cal Poly Holstein Herd, Michelle Van Ryn

Dairy Science

No abstract provided.