Animal Sciences Commons^™

Comparative- And Network-Based Proteomic Analysis Of Bacterial Chondronecrosis With Osteomyelitis Lesions In Broiler's Proximal Tibiae Identifies New Molecular Signatures Of Lameness, Jennifer Cook, Elizabeth Greene, Alison Ramser, Garrett J. Mullenix, Jalila S. Dridi, Rohana Liyanage, Robert F. Wideman Jr., Sami Dridi

Poultry Science Faculty Publications and Presentations

Bacterial Chondronecrosis with Osteomyelitis (BCO) is a specific cause of lameness in commercial fast-growing broiler (meat-type) chickens and represents significant economic, health, and wellbeing burdens. However, the molecular mechanisms underlying the pathogenesis remain poorly understood. This study represents the first comprehensive characterization of the proximal tibia proteome from healthy and BCO chickens. Among a total of 547 proteins identified, 222 were differentially expressed (DE) with 158 up- and 64 down-regulated proteins in tibia of BCO vs. normal chickens. Biological function analysis using Ingenuity Pathways showed that the DE proteins were associated with a variety of diseases including cell death, organismal …

Dietary Soy Galactooligosaccharides Affect The Performance, Intestinal Function, And Gut Microbiota Composition Of Growing Chicks, K. D. Teague, G. Tellez-Isaias, Jianmin Chai, V. Petrone-Garcia, C. N. Vuong, A. Blanch, S. H. Rasmussen, K. Brown, Jiangchao Zhao, S. J. Rochell

Poultry Science Faculty Publications and Presentations

The objective of this experiment was to investigate the effects of the dietary soy galactooligosaccharides (GOS), raffinose and stachyose, on performance, gastrointestinal health, and systemic stress in young broilers. Birds were fed a GOS-devoid diet based on soy protein isolate (SPI) or the SPI diet with 0.9, 1.8, 2.7, or 3.6% added stachyose and raffinose in a ratio of 4:1 at the expense of corn starch. These 5 treatments were administered to 10 replicate cages of 8 birds. Performance was measured weekly and excreta moisture, N retention, apparent metabolizeable energy, and complete blood cell counts were …

Enzyme-Treated Yeast Fed To Coccidia-Challenged Broiler Chickens, Emmanuel O. Alagbe, Hagen Schulze, Olayiwola Adeola

Graduate Industrial Research Symposium

There is a growing search for natural feed additives to alleviate the deleterious effects of coccidia infection in poultry. Research has shown that may serve as healthier alternatives to coccidiostats.

This study aimed to investigate the effect of enzyme-treated yeast (EY) on the performance, nutrient digestibility, intestinal morphology, and health of coccidia-challenged broiler chickens. 480 broiler chickens were allocated to 3 corn-soybean meal-based experimental diets with increasing concentrations of EY (0, 1, or 2 g/kg). Dietary EY enhanced nutrient utilization and augmented intestinal development in broiler chickens. However, dietary EY did not completely attenuate the adverse effects of a coccidia …

2022-2023 Christmas Bird Counts, Don Paseka

Nebraska Bird Review

Sixteen counts were run in the 2022-2023 CBC season. Because of weather and rescheduling issues, the Crawford, Harrison, and Harlan County counts were cancelled. Eight of the counts were completed during the first week of the count period and the other eight were scattered throughout the remainder of the count period. An early December cold snap seemed to drive many of the birds south, but the weather on actual count days was fairly benign: mostly dry with an average high of 34˚F and an average low of 19˚F and with three circles reporting light snow. The total number of species …

The Nebraska Bird Review: A Magazine Of Ornithology Of The Nebraska Region. Volume 91, Number 1. March 2023, Nebraska Ornithologists’ Union

Nebraska Bird Review

Winter Field Report, Dec. 2022 - Feb. 2023 by W. Ross Silcock

Annual Color Photo Section

Annual Treasurer's Report for 2022 by Jan Johnson

Subscription and Organization Information

Characterization Of Growth Patterns And Carcass Characteristics Of Male And Female Broilers From Four Commercial Strains Fed High Or Low Density Diets, C. J. Maynard, C. W. Maynard, A. R. Jackson, M. T. Kidd, S. J. Rochell, C. M. Owens

Poultry Science Faculty Publications and Presentations

Over the last few decades, the poultry industry has seen the emergence of various market segments that are beneficial for rearing various flock sizes. Two concurrent experiments consisting of 1,200 broilers each were conducted to evaluate the effects of broiler size and diet on the performance of four commercially available broiler strains, including 2 standard yielding (SY) and 2 high yielding (HY) strains. Within each experiment (Experiment 1: males, Experiment 2: females), a small bird (38 and 40 d processing) and big bird (47 and 54 d processing) debone market were targeted to give variable carcass …

Annual Treasurer's Report For 2022, Jan Johnson

Nebraska Bird Review

No abstract provided.

Winter Field Report, Dec. 2022 - Feb. 2023, W. Ross Silcock

Nebraska Bird Review

Since this is a Winter Seasonal Report, it seems appropriate to lead off with notable reports of occurrences that might be considered unexpected for midwinter, of which there were several. A range of species was involved, suggesting an encompassing cause such as climate change. Of interest were northerly wintering groups of waterfowl including Gadwall, Green-winged Teal, Canvasback, Ringnecked Duck, and Hooded Merganser. Reports of wintering Tundra Swans, Whitewinged Doves, and Lesser Goldfinches continue to increase, and numerous Redwinged Blackbirds in the northeast in midwinter were unexpected. Also quite unexpected were two Sandhill Cranes wintering in the east, the state’s first …

The Nebraska Bird Review Annual Color Photo Section

Nebraska Bird Review

Virginia Rail and chick, Washboard Road, North Platte, Lincoln Co., 28 June 2023. Photo by Boni Edwards.

American Coot, Eagle Scout Lake, Hall Co., April 2020. Photo by Colleen Childers.

Hudsonian Godwit, Marsh Wren Community Wetlands, Lancaster Co., 21 May 2022. Photo by Kirill Belashchenko.

American Avocet, N.P. Dodge Park, Omaha, Douglas Co., 2 May 2023. Photo by Thane Dinsdale

Female Cape May Warbler, photographer's backyard, Bellevue, Sarpy Co., 10 May 2022. Photo by Loren Padelford.

Male Cape May Warbler, Ft. Niobrara NWR, Cherry Co., 13 May 2022. Photo by Gordon Warrick.

Tennessee Warbler, Fontenelle Forest Wetlands, Sarpy Co., 24 …

Reducing Broiler Breast Myopathies Through Supplementation Of Guanidinoacetic Acid In Broiler Diets, C. J. Maynard, D. S. Nelson, S. J. Rochell, C. M. Owens

Poultry Science Faculty Publications and Presentations

Performance and processing advantages have been noted in meat animal production when excess arginine is available in the diet. In addition, dietary inclusion of guanidinoacetic acid (GAA) has provided insight on sparing arginine for broiler chickens. Therefore, a study was conducted to assess dietary inclusion of GAA on broiler breast myopathy prevalence. Dietary treatments were established using a control basal diet fed over four feeding phases and treatments were allocated as an additional supplementation of 0.06% (GAA 0.06) and 0.12% (GAA 0.12) in the form of CreAMINO (Alzchem Group, Trostberg, Germany). Bi-weekly BW, body weight gain (BWG …

Factors Affecting Presence And Occupancy Of Marabou Storks (Leptoptilos Crumeniferus) At Abattoirs And Slaughter Slabs Near Jinja, Uganda, Elena Arroway

DU Undergraduate Research Journal Archive

This study examined the factors affecting the presence and occupancy of marabou storks at abattoirs and slaughter slabs near Jinja, Uganda. The average number of storks per unit area was compared across different areas within one large abattoir, including an analysis of the presence of free food, human disturbance, and other scavenging birds (pied crow, piapiac, spur-winged lapwing, and cattle egrets). Occupancy across two abattoirs and one slaughter slab was compared and related to the number of animals slaughtered daily at the facility. Direct observations at abattoirs and slaughter slabs were conducted for ten minutes with five minutes between observations. …

Application Of Eugenol In Poultry To Control Salmonella Colonization And Spread, Mohammed Aljuwayd, Israa Abdullah Malli, Young Min Kwon

Poultry Science Faculty Publications and Presentations

The poultry sector is an essential component of agriculture that has experienced unprecedented growth during the last few decades. It is especially true for the United States, where the average intake of chicken meat increased from 10 pounds (4.5 kg) per person in 1940 to 65.2 pounds (29.6 kg) per person in 2018, while the country produced 113 billion eggs in 2019 alone. Besides providing nutrition and contributing significantly to the economy, chicken is also a natural reservoir of Salmonella, which is responsible for salmonellosis in humans, one of the significant foodborne illnesses around the globe. The increasing use of …

Comparison Of Corticosterone Concentrations Between Serum And Fecal Samples As A Measure Of Stress In Male Broilers., Kayla Weinacht

Murray State Theses and Dissertations

Stress has long been a topic of interest in the poultry industry due to its potential negative effects on chicken health and production of both eggs and meat. With increased global growth in egg and broiler production, more attention has been paid to the stressors that affect chickens in modern poultry operations, particularly environmental temperature and stocking density. Improving quantitative measurement of stress is always needed in poultry welfare research. In most studies, blood serum is measured for corticosterone, one of the biomarker hormones associated with stress in chickens. Methods of collecting blood serum are by their nature disruptive to …

Impacts Of Eastern Redcedar Juniperus Virginiana L. On Avian Communities In Eastern South Dakota, Jay Holm

Schultz-Werth Award Papers

Eastern redcedar (ERC) (Juniperus virginiana L.) is the most rapidly spreading woody species in the Northern Great Plains due to fire suppression, planting in windbreaks, and overgrazing. Its encroachment has converted already fragmented and/or degraded grassland into woodland, and there is concern that it may negatively impact grassland-obligate/dependent avian species. In this study, I evaluated avian community composition between ERC-encroached and non-encroached grasslands in eastern South Dakota. Six sites each of encroached grassland and non-encroached grassland were selected. Bird surveys were conducted by performing point counts placed equidistantly along a transect for two years. Presence and abundance of each species …

Bird Articulation Guide, Kelsey Blaze Miller

Undergraduate Theses, Professional Papers, and Capstone Artifacts

No abstract provided.

Evaluating The Effects Of Modifying Mixing And Pelleting Parameters On Feed Quality, Pellet Production Rate, And Broiler Performance, Lucas E. Knarr

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Chapter 2, an experiment was conducted to evaluate the effect of different mixer types (MTY) and mix times (MTI) on mix uniformity (MU). A 2 (mix time) x 3 (mixer type) factorial was utilized in this experiment to create six treatments, each mixed in three randomized complete blocks. Mix times were designated adequate or inadequate, were based on the manufacturers’ recommendation, and varied based on the mixer utilized. The mixer types utilized included a vertical mixer, horizontal mixer, and transfer mixing system. The transfer mixing system can be described as a batch-to-mix system …

Unraveling A Paradox Of Habitat Relationships: Scale-Dependent Drivers Of Temporal Occupancy-Abundance Relationships In A Cooperatively Breeding Bird, Natasha D.G. Hagemeyer, Mario B. Pesendorfer, Walter D. Koenig, Eric L. Walters

Biological Sciences Faculty Publications

Context Spatial occupancy and local abundance of species often positively covary, but the mechanisms driving this widespread relationship are poorly understood. Resource dynamics and habitat changes have been suggested as potential drivers, but long-term studies relating them to abundance and occupancy are rare. In this 34-year study of acorn woodpeckers (Melanerpes formicivorus), a cooperatively breeding species, we observed a paradoxical response to changes in habitat composition: despite a reduction in the availability of high-quality breeding habitat, the population increased considerably.

Objectives We investigated the role of annual variation in food availability and long-term changes in habitat composition as predictors …

Cluster Homolog Of Immunoglobulin-Like Receptor Genes In Chicken Immune Responses, Brandi A. Sparling

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores the identity and role of immunoglobulin-like (Ig-like) receptors in chickens, with focus on their implications in disease and disease progression. These receptors, wisely expressed across immune cells, interact with the major histocompatibility complex (MHC) class I molecules to modulate immune responses in mammals. Due to the insufficient representation of chicken Ig-like receptors in online databases, this study systematically annotates the chicken Cluster Homolog of Immunoglobulin-like Receptors (CHIR) genes using advanced bioinformatic techniques, aligning with the release of the 7th edition of the chicken genome assembly that comprises builds for a broiler and layer chicken. The analysis identifies …

The Effects Of Exogenous Enzyme Supplementation On The Performance, Bone Quality, And Nutrient Utilization Of Broiler Chickens, Megan Bauer

Theses and Dissertations--Animal and Food Sciences

Two experiments were conducted to examine the effects of Allzyme® Spectrum supplementation to a reduced nutrient diet on the growth performance, energy and nutrient digestibility and utilization, bone quality, and jejunal digesta viscosity in broiler chickens. Each experiment used 300 one-day old Cobb by-product breeder chicks with 5 treatments, 10 replicates, and 6 birds per replicate cage in a randomized complete block design. The only differences between the two experiments were experiment 1 lasted for 21 days using a corn-soybean meal-based diet and experiment 2 lasted for 22 days using a wheat-soybean meal-based diet. The 5 treatments included a positive …

Use Of Amino Acid Isotope Analysis To Investigate Capital Versus Income Breeding Strategies In Migratory Avian Species, Nico Lübcker, John P. Whiteman, Oliver N. Shipley, Keith A. Hobson, Seth D. Newsome

Biological Sciences Faculty Publications

1. Income and capital breeding represent opposing ends of a continuum of reproductive strategies. Quantifying nutrient allocation to reproduction is challenging, but recent advances in compound-specific stable isotope analysis hold promise for tracing the source of individual compounds allocated to reproduction.

2. Here, we describe a novel approach of using measured carbon (δ13C) and nitrogen (δ15N) isotope values of individual amino acids (AAs) in pectoral muscle of egg-laying females and egg yolk as a useful tool to quantify the reliance on income versus capital breeding in migrating species. We used white-fronted (Anser albifrons frontalis), lesser snow (A. …

Territory Inheritance And The Evolution Of Cooperative Breeding In The Acorn Woodpecker, Walter D. Koenig, Joseph Haydock, Hannah L. Dugdale, Eric L. Walters

Biological Sciences Faculty Publications

There are two main hypotheses for why offspring in cooperatively breeding taxa delay dispersal and remain on their natal territory rather than disperse. First, ecological constraints may force offspring to remain on their natal territory until a reproductive opportunity presents itself in an otherwise saturated habitat. Alternatively, delaying dispersal and helping kin may increase an offspring's inclusive fitness. One means by which offspring might enhance their direct fitness by delaying dispersal is by inheriting breeding status on their natal territory. Such territory inheritance regularly occurs in acorn woodpeckers, Melanerpes formicivorus, a species whose social groups consist of a cooperatively …

A Multi-Isotope Approach Reveals Seasonal Variation In The Reliance On Marine Resources, Production Of Metabolic Water, And Ingestion Of Seawater By Two Species Of Coastal Passerine To Maintain Water Balance, Lucas Navarrete, Nico Lübcker, Felipe Alvarez, Roberto Nespolo, Juan Carlos Sanchez-Hernandez, Karin Maldonado, Zachary D. Sharp, John P. Whiteman, Seth D. Newsome, Pablo Sabat

Biological Sciences Faculty Publications

Tracing how free-ranging organisms interact with their environment to maintain water balance is a difficult topic to study for logistical and methodological reasons. We use a novel combination of triple-oxygen stable isotope analyses of water extracted from plasma (δ¹⁶O, δ¹⁷O, δ¹⁸O) and bulk tissue carbon (δ¹³C) and nitrogen (δ¹⁵N) isotopes of feathers and blood to estimate the proportional contribution of marine resources, seawater, and metabolic water used by two species of unique songbirds (genus Cinclodes) to maintain their water balance in a seasonal coastal environment. We …

Lifetime Inclusive Fitness Effects Of Cooperative Polygamy In The Acorn Woodpecker, Walter D. Koenig, Sahas Barve, Joseph Haydock, Eric L. Walters

Biological Sciences Faculty Publications

Although over 50 y have passed since W. D. Hamilton articulated kin selection and inclusive fitness as evolutionary explanations for altruistic behavior, quantifying inclusive fitness continues to be challenging. Here, using 30 y of data and two alternative methods, we outline an approach to measure lifetime inclusive fitness effects of cooperative polygamy (mate-sharing or cobreeding) in the cooperatively breeding acorn woodpecker Melanerpes formicivorus. For both sexes, the number of offspring (observed direct fitness) declined while the number of young parented by related cobreeders (observed indirect fitness effect) increased with cobreeding coalition size. Combining these two factors, the observed inclusive …

The Birds And The Trees: Quantifying The Drivers Of Whitebark Pine Decline And Clark's Nutcracker Habitat Use In Glacier National Park, Vladimir Kovalenko

Graduate Student Theses, Dissertations, & Professional Papers

Whitebark pine (Pinus albicaulis), recently listed as threatened under the Endangered Species Act, is in steep decline in Glacier National Park, Montana, USA due to the non-native pathogen Cronartium ribicola, causal agent of the fatal disease white pine blister rust. A sample of the park’s population suggests that approximately 70 percent of whitebark pines have died, while 65 percent of the remaining trees are infected. Using landscape and climate variables, we show how geographic location, elevation, aspect, solar radiation, relative humidity, and snowpack interact with tree diameter to affect mortality, disease incidence, cone production, and regeneration. We also examine how …

Impacts Of Temperature And Salt Concentrations For Thermal Inactivation Of Salmonella In Moisture Enhanced Reconstructed Chicken Patties, Alik D. Browning

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this experiment is to determine the thermal kinetic parameters of Salmonella in moisture-enhanced, reconstructed ground chicken patties as affected by temperatures and salt concentrations. Salmonella is responsible for 35% of the foodborne illnesses associated with poultry products (Batz et al., 2011). Nonintact reconstructed chicken meat is mixed with brine solutions containing various salt and polyphosphate concentrations to increase water-holding capacity, decrease cooking losses, improve sensory tasting scores, and maintain the good quality of completed chicken products (Gill et al., 2004). Increasing salt concentrations within meats can increase the thermal inactivation of pathogens. In this study, Salmonella Typhimurium …

Antimicrobial Resistance Profile Of Escherichia Coli Isolated From Poultry Litter, M. J. Khong, A. M. Snyder, M. M. Young, N. L. Barbieri, S. L. Weimer

Poultry Science Faculty Publications and Presentations

Antimicrobial resistance is a threat to animal and human health. As a commensal and zoonotic bacterium, Escherichia coli has the potential to be a pathogenic source of antimicrobial resistance. The purpose of this study aimed to investigate the antimicrobial resistance profile of E. coli isolated from litter collected from pens in a broiler chicken experiment. E. coli was isolated from litter samples (n = 68 isolates) of 16 pens housing broilers to d 53 of age. Resistance to 10 antimicrobials was observed by disc diffusion. The presence of 23 antimicrobial and heavy metal resistance genes, O serogroups, and avian pathogenic …

Molecular And Phenotypic Characterization Of Escherichia Coli Isolated From Broiler Chicken Flocks In Mississippi, Priyanka Devkota

Theses and Dissertations

Avian pathogenic Escherichia coli (APEC) is the causative agent of colibacillosis. APEC causes significant economic losses to the poultry industry. In this study, 66 E. coli isolates were collected from broiler flocks across Mississippi and categorized as clinical and non-clinical isolates. Genotypic and phenotypic characterization of virulence factors and antimicrobial resistance profiles of these E. coli were examined. The data disclosed a higher prevalence of virulence genes in clinical isolates than in non-clinical isolates. High differences on the genotypic and phenotypic antimicrobial resistance among clinical and non-clinical isolates were observed. Whole genome sequences of avian E. coli elaborated a diverse …

Development Of A Novel Loop-Mediated Isothermal Amplification (Lamp) Assay For The Rapid Detection Of Clostridium Perfringens, Deepa Chaudhary

Theses and Dissertations

Clostridium perfringens (C. perfringens) causes diseases such as necrotic enteritis in poultry. Current methods for early detection of C. perfringens, including culture, and molecular techniques (PCR and qPCR), have logistical limitations in detecting pathogens on-site because they require specialized equipment and long sample preparation time. This study describes developing a highly sensitive on-site LAMP assay combined with a simple DNA extraction method to detect C. perfringens. Results showed that the LAMP assay was highly specific and sensitive with the optimal reaction conditions: 63°C for 30 mins with primer concentration of 1.2 mM FIP/BIP and 0.2 mM F3/B3. The HotSHOT method …

Insect Availability And Parental Care Behavior In A Common Bird, Cole Bourque

Symposium of Student Scholars

Populations of aerially insectivorous birds are declining throughout North America. Urbanization may indirectly contribute to this decline through its negative effects on populations of insects, an important food source in insectivores' diets, where low insect availability may be especially challenging for birds during breeding. How fluctuations in insect populations impact various species at higher trophic levels is an important area of current study for future conservation biology. Here, we examine whether nestling provisioning, brooding and guarding behaviors in a breeding bird—the European starling (Sturnus vulgaris)—may be altered depending on the relative insect availability in the environment. We found …

Remembering Clem Klaphake, Don Paseka

Nebraska Bird Review

Clement (Clem) Klaphake passed away on December 30, 2022, from complications of Parkinson’s disease, which he struggled with for several years. He was born in Melrose, Minnesota, in 1943 and grew up working on his parents’ dairy farm, which included the usual menagerie of other farm animals typical of that time. Perhaps it was the routine of milking twice a day, seven days a week, or the impressive scar on his leg from a boar tusk, but Clem was destined to leave the farm and become a life-long educator. ... After getting his degree in 1971, Clem was hired to …