Animal Sciences Commons^™

Amphisbaenian Head Movement And Burrowing Forces In Damp Granular Media, Jacob Newell

Williams Honors College, Honors Research Projects

Damp granular media is a difficult environment to study because it is both practically complex and it lacks equations which fully describe its behavior. In this study, an oscillatory lateral head movement and its effects while penetrating damp granular media were tested using a robophysical model. This experimental research was inspired by the burrowing behavior of the clade Amphisbaenia, a group of usually limbless squamates that employ a variety of different burrowing behaviors, but it can apply to a wide range of burrowers. This research could help with both human burrowing technologies and the further investigation of animal behaviors.

Rumen-Protected Methionine Supplementation Improves Lactation, Oxidative Status, And Immune Response During A Subclinical Mastitis Challenge In Lactating Dairy Cows., Anita Paz Lopez

Electronic Theses and Dissertations

Mastitis is a significant problem in the dairy industry, posing numerous challenges for dairy farmers worldwide. Mastitis refers to the inflammation of the mammary gland, primarily caused by bacterial infections. This condition affects the health and well-being of dairy cows and has severe economic implications for farmers and overall dairy production. The prevalence of mastitis substantially threatens milk quality, productivity, and profitability, making it a top concern in the dairy industry. Preventing mastitis in cows is crucial for maintaining the herd's health and ensuring highquality milk production. Nutritional strategies have recently gained greater attention due to a direct impact on …

Microencapsulation Of Lactobacillus Plantarum Mb001 And Its Probiotic Effect On Growth Performance And Gut Integrity Of Broile Rs, Sasi Vimon

Chulalongkorn University Theses and Dissertations (Chula ETD)

The objectives of this work were 1) to study the preparation and characterization of LPMB001/AG-AL, 2) todetermine the characteristics, stability and viability of LPMB001/AG-AL against simulated gastrointestinal tract, high temperature and storage conditions and 3) To evaluate the supplementation of LPMB001/AG-AL on growth performance,ileal digestibility, intestinal morphology and microbiome in broiler chickens. The present work declared microcapsule networks based on polymer matrices (sodium alginate, AL and agar, AG) and charges under ionic crosslink aw emulsion to encapsulate probiotics for extensive stability in adverse conditions. The microcapsule was studied on physicochemicalcharacteristics, swelling efficiency as well as release pertormance and viability under …

Screening Of Lactate Utilizing Bacteria And Their Beneficial Properties To Improve Energy Balance, Production And Ruminal Fermentation In Crossbred Dairy Goats, Saranporn Poothong

Chulalongkorn University Theses and Dissertations (Chula ETD)

Sixty-three strains were isolated from ruminal fluid of crossbred dairy goats. Based on phenotype and 16S rRNA gene, sixteenbacterial groups were identified and two novel species of Selenomonas were purposed in this study. The strain mPRGC5 was purposed as Selenomonas runimis and the strain mPRGC8 was named Selenomonas caprae. The antimicrobial activity of all the strains was determined.Ligilactobacillus salivarius MP3 showed antibacterial activity against Cutibacterium acnes subsp. acnes DSM 1897' and Kocuria thizophila Mill. The antimicrobial substance was genomically identified as enterolysin A. Furtherniore, L. salivarius MP3 presented probiotic properties and the potential antimicrobial activity against pathogens. The fourteen strains …

Investigating Of Candidate Genes Affecting Milk Production In Dairy Cattle Using Systematic Review And Whole Genome Sequencing Database, Xiaoxian Su

Chulalongkorn University Theses and Dissertations (Chula ETD)

Milk production traits in dairy cattle, such as milk yield, fat content, and protein levels, are pivotal to the economic success of the dairy industry. These traits not only determine the quality and quantity of milk produced but also directly impact the profitability of dairy operations. Consequently, a deep understanding of the genetic architecture underlying these traits is essential for the development of effective breeding programs aimed at enhancing milk production and quality. The purpose of this study is to identify potential candidate genes related to milk production in Thai dairy cattle through systematic review of genome wide association study …

Prioritization Of Candidate Genes For Mastitis Resistance Trait In Genome-Wide Association Studies (Gwas) Utilizing Multiple Methodologies In Thai Dairy Cattle, Nhu Le Uyen Vo

Chulalongkorn University Theses and Dissertations (Chula ETD)

Mastitis affects milk yield in dairy cattle worldwide, leading to economic losses. Recognized as a polygenic trait, mastitis resistance is influenced by genetic, environmental, and physiological factors. This study aimed to enhance the genetic architecture and selection of the candidate for mastitis resistance traits in Thai dairy cattle by several methods. The study consisted of two parts. In Part 1, the study is divided into 1A) collecting genes from a systematic review of published GWAS, 1B) Analyzing the gene list with the Thai dairy cattle whole genome sequencing database, and 1C) Performing pathway enrichment analysis to identify candidate genes. Further …

Analysis For Prioritizing Risk Status And Sustainable Utilization Of Cattle, Sheep, And Goat Breeds In Türkiye, Mesut Yildirir, Seyrani̇ Koncagül, Yasemi̇n Öner, Ayşe Oya Akin

Turkish Journal of Veterinary & Animal Sciences

Animal Genetic Resources (AnGR) are a warranty for future unknown conditions and needs. Due to this fact, sustainable use and conservation of AnGR have special importance, and to achieve reliable programs for these goals, the risk status of AnGR should be estimated accurately. The aim of this study was to estimate the risk status for three widely used domesticated livestock species of cattle, sheep, and goat in animal production: 6 cattle, 33 sheep, and 15 goat breeds were included in the analysis. Extinction probability (z) was taken as a criterion for the risk status of each breed. Data collection based …

Effect Of Lipid-Enriched Diet Associated With Bovine Recombinant Somatotropin On Superovulation Response And Embryo Recovery In Heifers, Richelle Matos Oliveira, Anselmo Domingos Ferreira Santos, Francisco Palma Rennó, Camilla Mendonça Silva

Turkish Journal of Veterinary & Animal Sciences

The current study aimed to evaluate the effects of soybean oil supplementation associated with the administration of bovine recombinant somatotropin (rBST) on the population of ovarian follicles, standardization of both the superovulation response and embryo recovery in heifers. We used 40 Holstein heifers submitted to a 2 × 2 factorial, forming the experimental groups: DC-SL (control diet plus saline solution application); OL-SL (diet with soybean oil plus saline application); DC-rBST (control diet plus application of 320 mg rBST) and OL-rBST (diet with soybean oil plus the application of 320 mg rBST). The animals received two applications of 500 mg of …

Phenotypic And Genotypic Characterization Of Antimicrobial Resistance In Commonly Isolated Salmonella Serovars From Chickens, Seyyi̇de Sariçam İnce, Mehmet Akan

Turkish Journal of Veterinary & Animal Sciences

Salmonellosis caused by Salmonella agents is the second most common zoonotic infection in humans. In recent years, Salmonella's increasing antimicrobial resistance (AMR) has been a concern. The major transmission route of Salmonella is consumption of contaminated poultry products. Therefore, monitoring of antimicrobial resistance in chicken-originated Salmonella is critically important. This study investigated AMR in four commonly isolated Salmonella serovars from chickens, namely Salmonella Enteritidis (S. Enteritidis), Salmonella Infantis (S. Infantis), Salmonella Kentucky (S. Kentucky), and Salmonella Typhimurium (S. Typhimurium). A total of 133 isolates were examined by phenotypic and genotypic AMR characterization. Resistance to 14 different antimicrobials and eight …

Are The Mandible Shapes Of Common (Paradoxurus Hermaphroditus) And Golden (Paradoxurus Zeylonensis) Palm Civets Different? A Pilot Study, İftar Gürbüz, Yasi̇n Demi̇raslan, Channa Rajapaksha, Devaka K. Weerakoon, Saminda Fernando

Turkish Journal of Veterinary & Animal Sciences

In this study adult common and golden palm civet mandibles were analysed by geometric morphometric method. For this purpose, the materials were photographed in accordance with the procedure and analysed using computer programs. No allometric component was detected in the comparison of the mandibles. The first principal component defined 28.657% of the total shape variation in the mandibles of the two groups that were analysed with a total of 19 landmarks. The samples were largely separated from each other in the principal component analysis. The shape differences were mostly in the caudal of the mandible. Also, in the common palm …

Occurrence Of Staphylococcus Aureus In Milk And Antibacterial Properties Of Some Herbal Extracts On Milk And Hospital-Acquired Strains, Reza Ebrahimi Hariry, Gülçi̇n Akca, Ayhan Fi̇lazi̇

Turkish Journal of Veterinary & Animal Sciences

Staphylococcus aureus is a remarkable infectious agent, and with the development of antibiotic resistance by S. aureus, finding new, effective, and safe alternative therapeutic options in animals and humans is crucially demanded. In this study, the occurrence of S. aureus was explored by collecting nonmastitis bovine milk samples first, and then the effects of herbal extracts on methicillinresistant S. aureus (MRSA) strains isolated from cow's milk and humans in the hospitals were investigated. The occurrences of S. aureus in bovine raw milk samples were 13.4%. All raw milk S. aureus isolates were fully susceptible to cefoxitin, ceftiofur, penicillin G, …

Efficacy Of Acacia Nilotica Bark Extract As An Alternative To Antibiotics In Broilers Challenged With Escherishia Coli From 15 To 28 Days, Muhammad Umer Zahid, Anjum Khalique, Shafqat Nawaz Qaisrani, Muhammad Ashraf, Ali Ahmad Sheikh, Muhammad Umar Yaqoob

Turkish Journal of Veterinary & Animal Sciences

The present experiment was conducted to compare the effect of Acacia nilotica bark extract and antibiotic on different biological parameters in broilers challenged with E. coli. Five hundred 2-week-old broiler chicks were divided into five treatments (Cont, NC, PC, AN-BE3, and AN-BE5) with ten replicates per treatment (10 chicks per replicate), and the experiment was extended over 2 weeks. All chicks, except that of Cont were challenged with E. coli (10$^{7}$ CFU/bird). Cont: basal diet only; NC: challenged with E. coli; PC: NC + Zinc Bacitracin 50 mg/kg diet; AN-BE3: NC + A. nilotica bark extract supplementation 0.3%; …

Effects Of Use Of Conventional And Sexed Semen On Conception Rate, Calf Sex, Calf Birth Weight, And Stillbirth In Holstein Heifers, Tahi̇r Bayril

Turkish Journal of Veterinary & Animal Sciences

Pregnancy rate is low in artificial insemination with sexed semen compared to conventional semen. This situation poses a major problem for dairy farms. The aim of this study was to determine the effects of semen type on conception rate, calf sex and birth weight, stillbirth, pregnancy loss, and postpartum maternal body weight (MBW) in Holstein heifers inseminated with sexed (n = 229), and conventional semen (n = 153). The female and male calf ratios of the heifers inseminated with sexed and conventional semen were found to be 88% to 54.8% and 12% to 45.2%, respectively. There was a significant difference …

Effects Of Free Capric Acid, Lauric Acid, And Coconut Oil Supplementation On Performance, Carcass, And Some Blood Biochemical Parameters Of Broiler Chickens, Mehmet Demi̇rci̇, Şevket Evci̇, Mehmet Aki̇f Karsli, Ali̇ Şenol

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to investigate the effects of the addition of free capric (decanoic, C10:0) and lauric (dodecanoic, C12:0) acids and coconut (Cocos nucifera) oil to the broiler diets on performance, carcass yield, internal organ weights and some blood parameters. A total of 144 day-old broiler chicks (Ross 308) were used in the study, and four main groups were formed with four subgroups containing equal number of chicks. The control (C) group was fed with basal diet without any supplementation and the experimental groups were fed with 0.4% free capric acid (CA)-, lauric acid (LA)-, …

Arterial Vascularization Of The Spleen In Merino Sheep, Melek Tosun, Şükrü Hakan Atalgin

Turkish Journal of Veterinary & Animal Sciences

In this study, it is aimed to reach detailed information about the anatomy of arteries in the sheep spleen, which can be used as an example and model for the success of planned or emergency surgical interventions related to the spleen. In spleen injuries, the spleen may be divided into two or three parts, or it may be limited to cracks on the spleen. In particular, it is aimed to protect the organs that have been damaged by trauma recently. Operations such as splenectomy and splenography are surgical procedures that require good knowledge of the pathways of artery and its …

Effects Of Feeding In Different Quality Rangelands On Meat Quality And Fatty Acids Composition In Angora Goat Kids And Young Bucks, Betül Zehra Sariçi̇çek

Turkish Journal of Veterinary & Animal Sciences

The effects of feeding in different quality rangelands on meat quality and fatty acids composition in Angora goat kids and bucks were determined. Twenty male Angora goat kids were selected from each farm that produces Angora goats in the area. There are three separate rangelands with previously determined quality classes (good, medium, and low-quality). In each farm, among these animals grazed completely on the rangeland, 7 heads from 4-month-old kids and 7 heads from 7-month-old bucks were selected and slaughtered. The Longissimus thoracis muscle was removed for meat quality measurement. The increased rangeland quality increased the protein content of kid's …

Examination Of Canine Herpesvirus-1 In Dogs By Serological, Molecular, And Pathological Methods, Serkan Yildirim, Fatma Gülten Bayraktar, Hakan Aydin, Yavuz Seli̇m Sağlam, Gi̇zem Eser, Muhammet Bahaeddi̇n Dörtbudak

Turkish Journal of Veterinary & Animal Sciences

In this study, it was aimed to examine Canine Herpesvirus-1 (CHV-1) with different diagnostic methods and investigate the pathological changes in positive samples. For this purpose, 100 dog uteruses of different ages and breeds, were removed by ovariohysterectomy and their blood samples were used as the study material. Enzyme-linked immunosorbent assay (ELISA) and Indirect Fluorescent Antibody Test (IFAT) for serological diagnosis, polymerase chain reaction (PCR) method for molecular diagnosis, immunohistochemistry (IHC) and immunofluorescence (IF) staining for immunopathological diagnosis were performed on the samples. Additionally, hematoxylin and eosin (HE) staining was performed to examine the histopathological changes in the CHV- 1-positive …

Genetic Parameters Of Growth, Reproductive, And Productive Characteristics In Anatolian Buffaloes, Yusuf Kaplan, Mustafa Tekerli̇

Turkish Journal of Veterinary & Animal Sciences

It was aimed to investigate the genetic parameters in growth, fertility, and productivity of Anatolian buffaloes maintained at private farms of Yozgat. A total of 1139 buffalo cows and their calves were used in the study. The weights at birth, weaning, 6th and 12th months of age, and daily live weight gains (DLWG) of buffalo calves born from 2015 to 2019 were recorded. Calving interval (CI) and service period (SP), lactation milk yield (LMY), milk yield per day of lactation period (MYPDLP), milk yield per day of calving interval (MYPDCI), peak yield (PY), a day at peak yield (DPY) and …

Modeling Of Individual Egg Weights Of Lohmann-Brown Layer Hens, Esra Yavuz, Samet Hasan Abaci, Kadi̇r Erensoy, Mustafa Şahi̇n

Turkish Journal of Veterinary & Animal Sciences

This study was carried out to determine the most suitable model for egg weights of the Cubic, Gompertz, Logistics, Gamma, Richard, Piecewise Quadratic, Orscov, and Sigmoaidal models, which are widely used in estimation. Lohmann-Brown Classic laying hens raised in Ondokuz Mayıs University Research and Application farm were used as animal material. In the modeling of egg weights of 351 layer hens raised in 3-storey cages, individual egg weight data measured at weekly intervals, 32 on the 1st floor, 17 on the 2nd floor, and 27 on the 3rd floor, were taken into account, and a total of 76 hens' individual …

The Effect Of Saccharomyces Cerevisiae As A Probiotic On The Nutrient Degradability Of Some Commonly Feedstuffs Used In Turkey, Muslimo Abdullahi Sheikh Hassan, Mehmet Aki̇f Karsli

Turkish Journal of Veterinary & Animal Sciences

The objective of this study was to evaluate the effects of Saccharomyces cerevisiae as a probiotic on in situ ruminal DM, OM, CP and starch degradability values in some energy and protein sources commonly used in ruminant nutrition. In this study, the in situ degradation kinetics and fractions of dry matter, organic matter, crude protein, and starch of 6 different feedstuffs (barley, wheat, corn, sunflower seed meal, cottonseed meal and soybean meal) commonly used in animal nutrition were determined. Three ruminally cannulated Bafra sheep were used in the experiment. The experiment was designed as carryover experimental design with two periods. …

Investigation Of The In Vitro Antioxidant Activity Of Fermented Tomato Pruning Residues And Its Effects On Biochemical And Hematological Parameters Of Rats, Bariş Denk, Beyza Aydoğan, İsmai̇l Bayram, Abdurrahman Fati̇h Fi̇dan

Turkish Journal of Veterinary & Animal Sciences

In the tomato greenhouse production process, a by-product emerges in the form of greenhouse residues. One of these residues is leaf pruning residue, which poses an environmental pollution problem in terms of waste management. The purpose of this work was to investigate the use of fermented tomato leaves (FTL) as an animal feed additive and its potential to mitigate the environmental pollution problem caused by greenhouse pruning residues. The pruning residues were fermented and their in vitro antioxidant activity (AOA) levels were analyzed using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH), reducing power (RP), and total phenolic content (TPC) assays. Wistar albino …

Investigation Of Snps In Bmp15 And Gdf9 Genes In "Çepni" And "Of" Sheep In The Black Sea Region Of Turkey, Koray Kirikçi

Turkish Journal of Veterinary & Animal Sciences

Sheep fertility genes have been studied in Turkey for many years. But so far it cannot be said that a satisfactory result has been achieved in terms of the number of detected polymorphisms on fertility genes. Exhibiting the genetic structure of local gene resources adapted to a particular region is very important for sustainability, conservation and breeding studies, and many other issues such as profitability. Therefore, the primary aim of this study was to investigate the single nucleotide polymorphisms (SNPs) on the BMP15 and GDF9 genes in two new Turkish sheep breeds, Çepni and Of. DNA sequencing was performed to …

The First Record Of Philophthalmus Lucipetus (Trematoda: Philophthalmidae) In The Great Egret (Ardea Alba) From Turkey, Mehmet Öztürk, Şi̇nasi̇ Umur

Turkish Journal of Veterinary & Animal Sciences

The genus Philophthalmus, Looss 1899 (Echinostoma: Philophthalmidae) is a cosmopolitan group of parasites that infects the eyes of birds and mammals, including humans. A great egret (Ardea alba) was found dead in Kızılırmak Delta, Samsun, Turkey. The bird was transported to the Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun Turkey, for a necropsy. In the parasitological examination, a trematode was detected in the eye, and then it was identified as Philophthalmus lucipetus. This is the first report of the zoonotic parasite P. lucipetus in Turkey.

Investigation To Examine The Profile Of Auditory Brainstem And Hearing Thresholds Using Tone Burst Audiometry Brainstem Response In A Preclinical Migraine Model, Megan Huelsing

Graduate Theses/Dissertations

Migraine is a disabling neurological disease that is characterized by prominent auditory symptoms, including hyperacusis, which is defined as extreme sensitivity to sounds. The goal of this study was to investigate changes in hearing sensitivity and neural changes in the brainstem by measuring neural activity of the auditory brainstem in an established preclinical model of chronic migraine. To induce a chronic migraine state within the trigeminal system, male and female Sprague Dawley rats were subjected to three known human risk factors including neck muscle tension and REM sleep deprivation that promote latent sensitization, and exposure to a pungent odor, which …

Integrating Robotics Into Wildlife Conservation: Testing Improvements To Predator Deterrents Through Movement, Stewart W. Breck, Jeffrey T. Schultz, David Prause, Cameron Krebs, Anthony J. Giordano, Byron Boots

United States Department of Agriculture Wildlife Services: Staff Publications

Background Agricultural and pastoral landscapes can provide important habitat for wildlife conservation, but sharing these landscapes with wildlife can create conflict that is costly and requires managing. Livestock predation is a good example of the challenges involving coexistence with wildlife across shared landscapes. Integrating new technology into agricultural practices could help minimize human-wildlife conflict. In this study, we used concepts from the fields of robotics (i.e., automated movement and adaptiveness) and agricultural practices (i.e., managing livestock risk to predation) to explore how integration of these concepts could aid the development of more effective predator deterrents.

Methods …

Assessment Of Habitat‐Specific Competition For Oral Rabies Vaccine Baits Between Raccoons And Opossums, James L. Helton, Jacob E. Hill, David A. Bernasconi, Wesley C. Dixon, Richard B. Chipman, Amy Gilbert, James C. Beasley, Guha Dharmarajan, Olin E. Rhodes Jr.

United States Department of Agriculture Wildlife Services: Staff Publications

Throughout the eastern United States, the National Rabies Management Program (NRMP) distributes oral rabies vaccine (ORV) baits to manage rabies virus circulation in raccoon (Procyon lotor) populations. The consumption of vaccine baits by non‐target species including Virginia opossums (Didelphis virginiana) may reduce the effectiveness of ORV programs, but competition for baits remains poorly quantified in many areas of the southeastern United States. We distributed placebo ORV baits injected with a biomarker across 4 land cover types (bottomland hardwood, upland pine, riparian, isolated wetland) on the Savannah River Site in South Carolina, USA, 2017–2019. We then trapped …

Producers’ Perceptions Of Large Carnivores And Nonlethal Methods To Protect Livestock From Depredation: Findings From A Multistate Federal Initiative, Keith M. Carlisle, Hailey E. Ellis, Colorado State University, Alan D. Bright

United States Department of Agriculture Wildlife Services: Staff Publications

We examined livestock producers’ perceptions of wolves, grizzly bears, black bears, and mountain lions, as well as their experiences with using nonlethal methods to protect livestock from depredation. All producers in the study received nonlethal predator management assistance in 2020 from USDA-APHIS-Wildlife Services (“WS”) pursuant to a federally funded initiative focused on the use of nonlethal methods to protect livestock. Such methods included assistance from range riders (i.e., individuals who monitor livestock and carnivores), fladry (i.e., flags hung from rope that serve as a repellent), and other audio/visual deterrents (e.g., Foxlights). The producers did not specifically seek nonlethal assistance from …

Genomic Analyses Of Gray Fox Lineages Suggest Ancient Divergence And Secondary Contact In The Southern Great Plains, Elizabeth M. Kierepka, Sophie Preckler-Quisquater, Dawn M. Reding, Antoinette Piaggio, Seth P.D. Riley, Benjamin N. Sacks

United States Department of Agriculture Wildlife Services: Staff Publications

The gray fox (Urocyon cinereoargenteus) lineage diverged from all other extant canids at their most basal node and is restricted to the Americas. Previous mitochondrial analysis from coastal populations identified deeply divergent (up to 1 Mya) eastern and western lineages that predate most intraspecific splits in carnivores. We conducted genotyping by sequencing and mitochondrial analysis on gray foxes sampled across North America to determine geographic concordance between nuclear and mitochondrial contact zones and divergence times. We also estimated the admixture within the contact zone between eastern and western gray foxes based on nuclear DNA. Both datasets confirmed that …

A Social Network Analysis Of Actors Involved In Wild Pig (Sus Scrofa) Management In Missouri, Hailey E. Ellis, Meredith J. Grady, Lauren Jaebker, Alan D. Bright, Keith M. Carlisle

United States Department of Agriculture Wildlife Services: Staff Publications

Wild pigs (Sus scrofa) cause significant damage to agriculture and native ecosystems and can transmit diseases to animals and people. Management responses designed to reduce population numbers are needed to mitigate these threats. Identifying networks of key actors, including the ways in which they interact, is valuable for purposes of better understanding opportunities or constraints that generate or impede effective management responses. The goal of our study was to understand the network of organizations, and the personnel working within them, that were active in wild pig management, research, or policy initiatives in Missouri during 2018–2020 by 1) identifying …

Effects Of Corn Supplementation On Serum And Muscle Microrna Profiles In Horses, Clarissa Carver, Jason E. Bruemmer, Stephen Coleman, Gabriele Landolt, Tanja Hess

United States Department of Agriculture Wildlife Services: Staff Publications

Laminitis associated with equine metabolic syndrome causes significant economic losses in the equine industry. Diets high in non-structural carbohydrates (NSC) have been linked to insulin resistance and laminitis in horses. Nutrigenomic studies analyzing the interaction of diets high in NSCs and gene expression regulating endogenous microRNAs (miRNA) are rare. This study's objectives were to determine whether miRNAs from dietary corn can be detected in equine serum and muscle and its impacts on endogenous miRNA. Twelve mares were blocked by age, body condition score, and weight and assigned to a control (mixed legume grass hay diet) and a mixed legume hay …