Agriculture Commons^™

A Transcriptome‑Snp‑Derived Linkage Map Of Apios Americana (Potato Bean) Provides Insights About Genome Re‑Organization And Synteny Conservation In The Phaseoloid Legumes, Jugpreet Singh, Scott R. Kalberer, Vikas Belamkar, Teshale Assefa, Matthew N. Nelson, Andrew D. Farmer, William J. Blackmon, Steven B. Cannon

Department of Agronomy and Horticulture: Faculty Publications

Apios (Apios americana; “apios”), a tuberous perennial legume in the Phaseoleae tribe, was widely used as a food by Native Americans. Work in the last 40 years has led to several improved breeding lines. Aspects of the pollination biology (complex floral structure and tripping mechanism) have made controlled crosses difficult, and the previous reports indicated that the plant is likely primarily an outcrosser. We used a pseudo-testcross strategy to construct a genetic map specific to the maternal parent. The map was built using single-nucleotide polymorphism markers identified by comparing the expressed sequences of individuals in the mapping population …

Can Bangladesh Produce Enough Cereals To Meet Future Demand?, J. Timsina, J. Wolf, N. Guilpart, L G. J. Van Bussel, P. Grassini, J. Van Wart, A. Hossain, H. Rashid, S. Islam, M. K. Van Ittersum

Department of Agronomy and Horticulture: Faculty Publications

Bangladesh faces huge challenges in achieving food security due to its high population, diet changes, and limited room for expanding cropland and cropping intensity. The objective of this study is to assess the degree to which Bangladesh can be self-sufficient in terms of domestic maize, rice and wheat production by the years 2030 and 2050 by closing the existing gap (Yg) between yield potential (Yp) and actual farm yield (Ya), accounting for possible changes in cropland area. Yield potential and yield gaps were calculated for the three crops using well-validated crop models and site-specific weather,management and soil data, and upscaled …

Parallel Altitudinal Clines Reveal Trends In Adaptive Evolution Of Genome Size In Zea Mays, Paul Bilinski, Patrice S. Albert, Jeremy J. Berg, James A. Birchler, Mark N. Grote, Anne Lorant, Juvenal Quezada, Kelly Swarts, Jinliang Yang, Jeffrey Ross-Ibarra

Department of Agronomy and Horticulture: Faculty Publications

While the vast majority of genome size variation in plants is due to differences in repetitive sequence, we know little about how selection acts on repeat content in natural populations. Here we investigate parallel changes in intraspecific genome size and repeat content of domesticated maize (Zea mays) landraces and their wild relative teosinte across altitudinal gradients in Mesoamerica and South America. We combine genotyping, low coverage whole-genome sequence data, and flow cytometry to test for evidence of selection on genome size and individual repeat abundance. We find that population structure alone cannot explain the observed variation, implying that …

Strawberry Cultivars Vary In Productivity, Sugars And Phytonutrient Content When Grown In A Greenhouse During The Winter, Ellen T. Paparozzi, George Meyer, Vicki Schlegel, Erin Blankenship, Stacy A. Adams, M. Elizabeth Conley, Benjamin A. Loseke, Paul Read

Department of Agronomy and Horticulture: Faculty Publications

In many areas of the US, fresh locally grown berries are not available during the winter. With this in mind, a research study comprised of three experiments was conducted focused on cultivar selection for berry yield, number, sweetness and phytonutrient content. Using a capillary mat system with under bench heating within a double-layer polyethylene greenhouse, strawberries were grown in the Great Plains Region of the US during the winter. During experiment 1, 12 cultivars were grown; berries were weighed, counted and analyzed for sugars and phytonutrients. “Albion” plants produced a high number/mass of berries, had relatively high sugar content but …

A Novel Lidar-Based Instrument For High-Throughput, 3d Measurement Of Morphological Traits In Maize And Sorghum, Suresh Thapa, Feiyu Zhu, Harkamal Walia, Hongfeng Yu, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

Recently, imaged-based approaches have developed rapidly for high-throughput plant phenotyping (HTPP). Imaging reduces a 3D plant into 2D images, which makes the retrieval of plant morphological traits challenging. We developed a novel LiDAR-based phenotyping instrument to generate 3D point clouds of single plants. The instrument combined a LiDAR scanner with a precision rotation stage on which an individual plant was placed. A LabVIEW program was developed to control the scanning and rotation motion, synchronize the measurements from both devices, and capture a 360^◦ view point cloud. A data processing pipeline was developed for noise removal, voxelization, triangulation, and plant …

Genomic Prediction Accuracy For Switchgrass Traits Related To Bioenergy Within Differentiated Populations, Jason D. Fiedler, Christina Lanzatella, Serge J. Edme, Nathan A. Palmer, Gautam Sarath, Rob Mitchell, Christian M. Tobias

Department of Agronomy and Horticulture: Faculty Publications

Background: Switchgrass breeders need to improve the rates of genetic gain in many bioenergy-related traits in order to create improved cultivars that are higher yielding and have optimal biomass composition. One way to achieve this is through genomic selection. However, the heritability of traits needs to be determined as well as the accuracy of prediction in order to determine if efficient selection is possible.

Results: Using five distinct switchgrass populations comprised of three lowland, one upland and one hybrid accession, the accuracy of genomic predictions under different cross-validation strategies and prediction methods was investigated. Individual genotypes were collected using GBS …

Wheat Height Estimation Using Lidar In Comparison To Ultrasonic Sensor And Uas, Wenan Yuan, Jiating Li, Madhav Bhatta, Yeyin Shi, P. Stephen Baenziger, Yufeng Ge

Department of Agronomy and Horticulture: Faculty Publications

As one of the key crop traits, plant height is traditionally evaluated manually, which can be slow, laborious and prone to error. Rapid development of remote and proximal sensing technologies in recent years allows plant height to be estimated in more objective and efficient fashions, while research regarding direct comparisons between different height measurement methods seems to be lagging. In this study, a ground-based multi-sensor phenotyping system equipped with ultrasonic sensors and light detection and ranging (LiDAR) was developed. Canopy heights of 100 wheat plots were estimated five times during a season by the ground phenotyping system and an unmanned …

Root And Shoot Contribution To Carbon And Nitrogen Inputs In The Topsoil Layer In No-Tillage Crop Systems Under Subtropical Conditions, Marciel Redin, Sylvie Recous, Celso Aita, Bruno Chaves, Ismael Cristiano Pfeifer, Leonardo Mendes Bastos, Getulio Elias Pilecco, Sandro Jose Giacomini

Department of Agronomy and Horticulture: Faculty Publications

Recycling of carbon (C) and nitrogen (N) from plants into soils is decisive for maintaining soil organic matter and soil fertility. Therefore, we quantified plant biomass and C and N in the shoots and roots from the topsoil layer for a wide range of annual crops grown under subtropical conditions. We grew 26 species, 13 main crops, and 13 cover crops, in the field in standard sowing arrangements. Root biomass was recovered from the 0.00-0.20 m soil layer at flowering, and shoot biomass was measured at flowering for all crops and at maturity only for the main crops. Root dry …

Method For The Production Of High Saturated, Low Polyunsaturated Soybean Oil, Thomas E. Clemente, Hyunwoo Park, Edgar B. Cahoon, Hanh Nguyen

Department of Agronomy and Horticulture: Faculty Publications

Methods of genetically modifying soybean plants to alter the fatty acid properties of the oil are described.

Active-Optical Reflectance Sensing Corn Algorithms Evaluated Over The United States Midwest Corn Belt, G. M. Bean, N. R. Kitchen, J. J. Camberato, R. B. Ferguson, F. G. Fernandez, D. W. Franzen, C. A. M. Laboski, E. D. Nafziger, J. E. Sawyer, P. C. Scharf, J. Schepers, J. S. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

Uncertainty exists with corn (Zea mays L.) N management due to year-to-year variation in crop N need, soil N supply, and N loss from leaching, volatilization, and denitrification. Activeoptical reflectance sensing (AORS) has proven effective in some fields for generating N fertilizer recommendations that improve N use efficiency, but locally derived (e.g., within a US state) AORS algorithms have not been tested simultaneously across a broad region. The objective of this research was to evaluate locally developed AORS algorithms across the US Midwest Corn Belt region for making in-season corn N recommendations. Forty-nine N response trials were conducted across …

Framing Contemporary U.S. Wild Horse And Burro Management Processes In A Dynamic Ecological, Sociological, And Political Environment, J. Derek Scasta, Jacob D. Hennig, Jeffrey L. Beck

Human–Wildlife Interactions

The Wild Free-Roaming Horses and Burros Act (WFRHBA) of 1971 established all “unbranded or unclaimed” equids on U.S. public lands as “living symbols of the historic and pioneer spirit of the West.” Today, >72,000 feral horses (Equus ferus caballus) and burros (E . asinus ; WHB) live on western U.S. public rangelands. The number of WHBs exceeds the Bureau of Land Management’s maximum Appropriate Management Level (AML) of 26,715 by a factor of approximately 2.7 and has nearly doubled from 2007–2015. The AML was set to balance WHB numbers with rangeland health and support other uses such as wildlife habitat …

Spatial Ecology Observations From Feral Horses Equipped With Global Positioning System Transmitters, Jacob D. Hennig, Jeffrey L. Beck, J. Derek Scasta

Human–Wildlife Interactions

Our understanding of the spatial ecology of feral horses (Equus ferus caballus ) and burros (E . asinus ) in the United States is limited. Robust location data are needed to better understand the permeability of Bureau of Land Management Herd Management Area boundaries, relative to feral horse movement patterns and home ranges. To increase our understanding of feral horse movement, in February to March 2017, we deployed global positioning system (GPS) collars on 14 females ≥5 years old that were captured in the Adobe Town Herd Management Area (ATHMA) of southcentral Wyoming, USA. Herein, we report initial results from …

Establishing Growing Degree Day Estimates To Predict Critical Growth Stages In Soft Red Winter Wheat, Ethan J. Snyder

Theses and Dissertations--Plant and Soil Sciences

Predicting developmental growth stages in soft red winter wheat (Triticum aestivum L.) (SRWW) could improve agronomic management in Kentucky. However, predicting SRWW development is complex due to vernalization requirement and photoperiod sensitivity differences of cultivars. The objectives of this study are to (1) determine ability of Kompetitive Allele Specific PCR (KASP) genotyping to predict phenotype; (2) determine the relative vernalization requirement (RVR) of 50 SRWW cultivars in a greenhouse (GH) assay; and (3) measure growing degree-days (GDD) required by cultivars to reach eight growth stages in a field assay. Fifty SRWW cultivars were characterized with 14 KASP markers for …

Bacterial Inoculants, Endophytic Bacteria And Their Influence On Nicotiana Physiology, Development And Microbiome, Andrea Marisa Sanchez Barrios

Theses and Dissertations--Plant and Soil Sciences

Soil and root microbial communities have been studied for decades, and the incorporation of high-throughput techniques and analysis has allowed the identification of endophytic/non-culturable organisms. This has helped characterize and establish the core microbiome of many model plant species which include underground and aboveground organs. Unfortunately, the information obtained from some of these model plants is not always transferable to other agronomic species. In this project, we decided to study the microbiome of the Nicotiana genus because of its importance in plant physiological and plant-microbe interactions studies. The data obtained was used as baseline information that allowed us to better …

Chemical Topping Burley Tobacco, Mitchell D. Richmond

Theses and Dissertations--Plant and Soil Sciences

The act of topping tobacco (Nicotiana tabacum L.) involves the removal of the terminal bud or inflorescence of the tobacco plant. This practice ordinarily is accomplished by manually removing the top of each tobacco plant in an entire field which is labor intensive and costly. Chemical topping utilizes sucker control products to inhibit the terminal bud and axillary bud growth without manually removing the top of the tobacco plant. There were several research objectives in order to determine the utility of a chemical topping system: 1) determine if burley tobacco could be chemically topped with currently registered suckercide products …

Can Increasing Grass-Fungal Endophyte Symbiotic Diversity Enhance Grassland Ecosystem Functioning?, Mahtaab Bagherzadeh

Theses and Dissertations--Plant and Soil Sciences

The relationship between biodiversity and ecosystem functioning is important in maintaining agroecosystem sustainability. Plant-microbe symbioses, such as exists between the grass tall fescue (Schedonorus arundinaceum) and the asexual fungal endophyte Epichloë coenophiala, can be utilized to enhance agroecosystem functions, such as herbivore resistance. “Novel” E. coenophiala strains that vary in the production of mammal- and insect-toxic compounds have been identified, inserted into tall fescue cultivars, and are planted in pastures globally. Novel fungal endophyte-tall fescue associations may have divergent ecosystem function effects. This study assessed effects of different fescue-endophyte symbiotic combinations on pasture ecosystem function, including aboveground …

Engineering Zinc Oxide Nanoparticles To Be Used As Nanofertilizers, Zeinah Elhaj Baddar

Theses and Dissertations--Plant and Soil Sciences

Zinc deficient soils, or soils with low Zn bioavailability, are widespread, which exacerbates Zn deficiency in human as crops grown on these soils have low Zn content. Often crop yields are also compromised. Fertilizers based on soluble Zn salts often have limited efficacy in such soils. In this research, we evaluate the performance of polymer coated and bare ZnO nanoparticles (NPs) in an attempt to overcome limitations of soluble Zn salts in alkaline soils. We first synthesized 20-30 nm bare ZnO NPs with different surface chemistries to impart colloidal stability to the particles. Bare ZnO were treated in phosphate solution …

Characterizing Nitrogen Loss And Greenhouse Gas Flux Across An Intensification Gradient In Diversified Vegetable Systems, Debendra Shrestha

Theses and Dissertations--Plant and Soil Sciences

The area of vegetable production is growing rapidly world-wide, as are efforts to increase production on existing lands in these labor- and input-intensive systems. Yet information on nutrient losses, greenhouse gas emissions, and input efficiency is lacking. Sustainable intensification of these systems requires knowing how to optimize nutrient and water inputs to improve yields while minimizing negative environmental consequences. This work characterizes soil nitrogen (N) dynamics, nitrate (NO₃¯) leaching, greenhouse gas emissions, and crop yield in five diversified vegetable systems spanning a gradient of intensification that is characterized by inputs, tillage and rotational fallow periods. The study systems …

Environmental Mycobacterium Avium Subsp. Paratuberculosis Hosted By Free-Living Amoebea, Ascel Samba-Louaka, Etienne Robino, Thierry Cochard, Maxime Branger, Vincent Delafont, Willy Aucher, Wilfrid Wambeke, John Bannantine, Franck Biet, Yann Héchard

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mycobacterium avium subsp. paratuberculosis is responsible for paratuberculosis in animals. This disease, leading to an inflammation of the gastrointestinal tract, has a high impact on animal health and an important economic burden. The environmental life cycle of M. avium subsp. paratuberculosis is poorly understood and several studies suggest that free-living amoebae (FLA) might be a potential environmental host. FLA are protozoa found in water and soil that are described as reservoirs of pathogenic and non-pathogenic bacteria in the environment. Indeed, bacteria able to survive within these amoebae would survive phagocytosis from immune cells. In this study, we assessed the in …

A Mycobacterium Avium Subsp. Paratuberculosis Rela Deletion Mutant And A 35 Kda Major Membrane Protein Elicit Development Of Cytotoxic T Lymphocytes With Ability To Kill Intracellular Bacteria, Gaber S. Abdellrazeq, Mahmoud M. Elnaggar, J. P. Bannantine, Kun Park, Cleverson D. Souza, Brian Backer, Victoria Hulubei, Lindsay M. Fry, Samy A. Khaliel, Helmy A. Torky, David A. Schneider, William C. Davis

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Efforts to develop live attenuated vaccines against Mycobacterium avium subspecies paratuberculosis (Map), using indirect methods to screen Map deletion mutants for potential efficacy, have not been successful. A reduction in the capacity to survive in macrophages has not predicted the ability of mutants to survive in vivo. Previous studies for screening of three deletion mutants in cattle and goats revealed one mutant, with a deletion in relA (ΔMap/relA), could not establish a persistent infection. Further studies, using antigen presenting cells (APC), blood dendritic cells and monocyte derived DC, pulsed with ΔMap/relA or a 35 kDa Map membrane protein (MMP) revealed …

Sustainability At Sit: A Look At The Past, A Plan For The Future, Taliesin Haugh

Capstone Collection

Climate change threatens our world and way of life. Intelligent development and investment could mitigate the worst threats of climate change, while simultaneously providing continuous growth for the global economy. The New Climate Economy proposes efforts to combat this ecological collapse that would result in $30 trillion in new annual economic growth by 2030. Stockholm Resilience Center agrees, giving a framework based on global ecological systems that calls for five critical tasks that can bring growth and stability: Renewable energy

Sustainable local food production

New development models, based on what has worked globally

Reduction of wealth inequity

Education, health, and …

Building A Custom 4-Row Planter, Jacob Larkin

A with Honors Projects

This brief essay describes the processes taken to build a custom 4-row planter with the precision Agriculture Department and their company partner, Helena Chemical.

Measuring The Influence Of Bedding On Soil Nitrogen Losses And Corn Crop Nitrogen Characteristics For Fall Applied Solid Beef Cattle Manure In Eastern South Dakota, Mukesh Mehata

Electronic Theses and Dissertations

Nitrogen (N) is a major component of chlorophyll which plays a key role in the photosynthesis process in crops. The N is one of the highest demanded nutrients by all plants for their growth and reproduction. Manure or inorganic fertilizer is often applied to fulfill the crops’ N demand. However, the applied N sources have the potential of N losses in different forms from the soil volume in many ways such as ammonia (NH₃) volatilization, aerial nitrous oxide (N₂O) loss, nitrate (NO₃ --N) leaching, and runoff and/or erosion. Soil fertility, crop yield, water quality, and …

Evaluation Of Dietary Phytochemicals As Rumen Modifiers In Lactating Dairy Cows, Sonia Rodriguez Jimenez

Electronic Theses and Dissertations

Phytochemicals such as tannins included in dairy cow nutritional programs, as a natural feed additive, promise to improve rumen bypass protein and consequently reduce the feeding cost by decreasing the amount of CP needed in the ration. Additionally, if tannins are fed along with high quality protein sources, this could improve the AA profile reaching the small intestine and potentially improving health and performance of dairy cows. The black pepper, specifically its alkaloid piperine, is a nontoxic, natural dietary compound with a broad range of physiological activity. Lactating dairy cows ingesting these phytochemicals (tannin extracts and black pepper) will potentially …

Efficiency Of Utilizing Standardized Ileal Digestible Lys And Thr For Whole Body Protein Retention In Pregnant Gilts During Early, Mid And Late Gestation, Ron Aldwin Sapin Navales

Electronic Theses and Dissertations

In pregnant pigs, amino acid (AA) requirements represent the sum of those required for maintenance functions, protein retention and efficiency of utilizing AA intake for the aforementioned body processes. The NRC (2012) model assumed AA efficiency is constant across period of gestation; however this is not reflective of the changes in metabolic demand during gestation. Therefore, two experiments were conducted to evaluate the efficiency of utilizing SID Lys and Thr for whole body protein retention (kSIDLys and kSIDThr) in pregnant gilts during early, mid and late gestation. Three 12 d N-balance studies were conducted to represent different periods of gestation. …

A Quantitative Environmental Assessment Of Incorporating Torrefaction Into Farming Enterprises In Eastern South Dakota, Dinesh Fuyal

Electronic Theses and Dissertations

The use of renewable energy sources has been increasing in the recent years due to population growth and environmental concerns. Biomass is a promising energy source that can be used to produce biofuels or torrefied pellets. Torrefied biomass may be used in power plants, industrial and residential heating, feedstocks for gasification, air and water filtrating, and soil amendment. The interest of torrefied pellets as energy sources for various applications has been increased in the recent years due to the concerns about energy security and environmental issues. This study focuses on the economic and environmental assessment of agricultural feedstocks like corn …

Agri 115: Biotechnology – Food, Health And Environment, A Peer Review Of Teaching Project Benchmark Portfolio, Leah Sandall

UNL Faculty Course Portfolios

No abstract provided.

Aecn 141- Introduction To The Economics Of Agriculture, Timothy Meyer

UNL Faculty Course Portfolios

Course portfolio for AECN 141- Introduction to the Economics of Agriculture, University of Nebraska-Lincoln.

Body Image In Adolescence, Jaqueline Neid-Avila, Brittany Bingeman

All Current Publications

This fact sheet addresses risk factors and protective factors in order to help adolescents navigate this critical time frame.

Como Plantar Un Huerto Para Preparar Salsas, Dan Drost, Celina Wille

All Current Publications

No abstract provided.