Life Sciences Commons^™

Comparison Of Methods For Amphetamine Enantiomer Analysis Using Hplc And 1h-Nmr, Martin Mai

Student Theses

A molecule is chiral if it cannot be superimposed on its mirror image. Chiral drugs exist as these mirror image enantiomers, which can differ in their pharmacological effects. When a drug’s active molecule exists as a pair of enantiomers, the relative ratio of each enantiomer in the final product can provide information about the source of production for this drug, i.e., whether the drug was legally or illegally produced. However, typical detection methods may not differentiate between enantiomers of a chiral molecule. This thesis study compares three different methods for enantiomer detection of amphetamine, which is a drug often prescribed …

Variation In Sorghum Aphid (Melanaphis Sorghi) 1 Populations Translates Into Life History Traits On Sorghum-Sudangrass (Sorghum´ Drummondii), Neetu Khanal, Christopher J. Vitek, Rupesh R. Kariyat

School of Integrative Biological & Chemical Sciences Faculty Publications

Sorghum aphid (Melanaphis sorghi; SA) (Theobald) is a new invasive pest of sorghum first reported in the USA in 2013. Since then, the use of synthetic pesticides and resistant cultivars have increased, which has caused levels of differences in the populations of SAs and led to the potential emergence of new biotype(s) of SA. However, most research has concentrated on comparing SA fed on sorghum, sugarcane, and other hosts, which may have obscured any population differences that may exist within sorghum. To understand this, we collected three populations of SA that fed on sorghum from three different locations around …

Evaluating The Stress System Of The Grieving Brain: Corticotropin-Releasing Factors And Partner Loss In Female Coyotes, Rachel Tong Xin Ning

Undergraduate Honors Capstone Projects

Forming social bonds is fundamental in helping us foster connections with others. The loss of a loved one often results in grief, stress, and loneliness, and the stress response system of the body has been implicated in the physiological symptoms associated with grieving. Corticotropin releasing factor (CRF) is the hormone that initiates the stress response in the body and acts at two different receptor subtypes CRF receptor (CRFR)1 and CRFF2. Many studies on CRF and social loss have been conducted in monogamous prairie voles, but studies in longer-lived monogamous mammals could improve understanding of the effects of losing pair bonds. …

The Role Of Lifr Signaling In Innate Immunity, Laura Kahn Serrudo

Dissertations and Theses (Open Access)

The localized signals that bone marrow (BM) hematopoietic stem cells (HSCs) and immune cells receive are critical in regulating their responses in the steady state and inflammation. Monocytes are an abundant and key population in the innate immune response that originates in the BM, and they circulate to monitor the periphery. Monocytes are composed of many subsets that have essential and specific functions, including swift egress from the BM, production of pro-inflammatory cytokines, and differentiation into monocyte-derived dendritic cells (MoDCs), which are required for efficient pathogen eradication.

Here, we uncovered LIFR (leukemia inhibitory factor receptor) restrains low-grade chronic inflammation in …

A Pan-Cancer Single-Cell Analysis Of Intratumoral Copy Number Diversity And Evolution, Hanghui Ye

Dissertations and Theses (Open Access)

Aneuploidy is a hallmark of human cancers, with many copy number aberrations (CNAs) being associated with disease progression. Previous studies have revealed extensive inter-patient heterogeneity (IPH) in copy number profiles. However, the extent of intratumoral heterogeneity (ITH) and its evolutionary dynamics remain poorly understood.

To address these gaps, we developed Acoustic Cell Tagmentation (ACT), an advanced single-cell single-molecule DNA sequencing (scDNA-seq) technology, to resolve the copy number substructure of human tumors and investigate the evolution of aneuploidy. Applying ACT to eight triple-negative breast cancer (TNBC) patients to profile 9,765 aneuploid tumor cells, we discovered that following initial punctuated copy number …

Oncolytic Viruses Enhance Long-Term Nk Cell Anti-Tumor Cytotoxicity Through Ap-1 And Irf Pathway Activation, Xin Ru Jiang

Dissertations and Theses (Open Access)

Natural killer (NK) cell therapeutics have emerged as a promising strategy in adoptive cellular therapy. While genetic modifications of NK cells can enhance their antitumor activity, modulating the tumor to augment NK cell recognition and function remains underexplored. In this study, we investigated the combination of NK cells with oncolytic viruses to treat solid tumors, specifically pancreatic ductal adenocarcinoma and glioblastoma. We demonstrated that infecting tumor cells with the oncolytic adenovirus Delta-24-RGD significantly increases their susceptibility to NK cell cytotoxicity, driven by a hyperactivated NK cell phenotype characterized by elevated expression of activating receptors and cytotoxicity markers. In a patient-derived …

Investigating The Rare Aneuploid Cells In Normal Breast And Therapeutic Resistance In Triple Negative Breast Cancer Using Single Cell Genomics, Yiyun Lin

Dissertations and Theses (Open Access)

Aneuploid epithelial cells are common in breast cancer, however their presence in normal breast tissues is not well understood. To address this question, we applied single cell DNA sequencing to profile copy number alterations (CNAs) in 83,206 epithelial cells from breast tissues of 49 healthy women and single cell DNA&ATAC co-assays to 19 women. Our data shows that all women harbored rare aneuploid epithelial cells (median 3.19%) that increased with age. Many aneuploid epithelial cells (median 82.22%) in normal breast tissues underwent clonal expansions and harbored CNAs reminiscent of invasive breast cancers (gains of 1q, losses of 10q, 16q and …

Local Or Systemic Antibody-Targeted Sting Activation Drives Pro- Inflammatory Stromal Metabolic Changes And Downregulates C-Myc Oncoprotein, Akash Boda

Dissertations and Theses (Open Access)

The past decade has witnessed remarkable advances in the field of cancer immunotherapy. Immune checkpoint blockade (ICB) has emerged as a cornerstone, achieving long-lasting tumor regression in several types of cancer. However, its effectiveness is often limited, particularly in certain tumor types. Immunologically "cold" tumors, such as pancreatic ductal adenocarcinoma (PDAC), remain clinically unresponsive to ICB, presenting a significant challenge in oncology. Amidst these challenges, the stimulation of the STING pathway via small molecule synthetic agonists has surfaced as a promising strategy. Acting as an in-situ vaccine, STING activation has the potential to transform the immune landscape of cold tumors …

The Role Of Ganglioside Gd2 And Gd3 Synthase (St8sia1) In The Regulation Of Immune Suppression In Breast Cancer, Bolutyfe Oderinde

Dissertations and Theses (Open Access)

Gangliosides are acidic glycosphingolipids involved in cell adhesion, proliferation, and modulation of signal transduction pathways. It has been reported that tumor-shed gangliosides influence the activity of immune cells including macrophages, NK cells, and T cells. GD3 synthase (GD3S) is the key enzyme that regulates the biosynthesis of the b and c series gangliosides, particularly GD3 and GD2, and studies have shown that GD3S is upregulated in most tumors and plays a role in tumor progression. Similarly, we have previously found GD3S to be significantly upregulated in GD2+ breast cancer stem cells (BCSC) compared to GD2- cells, and the knockdown of …

Assessing The Temporal Role Of Mir-200 Loss In Murine Models Of Nsclc, Jared Fradette

Dissertations and Theses (Open Access)

Lung cancer is the leading cause of cancer related deaths in the United States, with non-small cell lung cancer (NSCLC) making up a majority of new diagnoses. Metastasis is the big killer in NSCLC and is driven by epithelial-mesenchymal transition (EMT) and immune evasion. The microRNA 200 family is a master regulator of EMT and is implicated in immune regulation. In this study we have developed a novel genetically engineered mouse model (GEMM) and derived primary cell lines from them to explore the role of microRNA-200 in early EMT and immune changes. Our model combines conditional activation of Kras^{G12D …}

The Impact Of Supercritical Carbon Dioxide Processing On The Aroma And Physicochemical Properties Of Soybean Flour, Sumanjot Kaur, Ali Ubeyitogullari

Food Science Faculty Publications and Presentations

Soybeans are rich in protein but have limited food applications due to their undesirable volatile compounds, negatively affecting their flavor and consumer acceptance. This study aimed to extract lipids and volatile compounds from soybean flour using supercritical carbon dioxide (SC-CO₂) and investigate the impact of extraction conditions on the properties of soybean flour, including particle size distribution, composition, color, and rheological and textural qualities. Similar lipid extraction yields (∼20 %) were obtained with SC-CO₂ and conventional hexane extractions. The volatile compound concentration of the soybean flour was reduced after SC-CO₂ extraction (0.63–1.00 µg/g) as compared to …

Size Matters: The Characterization Of Telomere Function In Germ Cell Development In Danio Rerio, Jessica Macneil

Graduate Doctoral Dissertations

Infertility is a growing problem worldwide. According to the WHO, 48 million couples across the globe suffer from infertility and many couples turn to Assisted Reproductive Technology (ART) to become pregnant. One readout of gamete quality and successful ART is telomere integrity because telomeres are necessary for chromosome movements during meiosis so that the segregation of chromosomes happens correctly. Telomeres are well known for their role in aging; however, they play an important and understudied role during meiosis. In meiosis, telomeres attach to the nuclear envelope (NE) via a protein chain spanning the NE that interacts with cytoplasmic motor proteins, …

Elevation And Population Size Effects On The Survival Of Drosera Rotundifolia, Jessica Williams

Electronic Theses and Dissertations

Carnivorous plants face significant conservation challenges due to habitat destruction, climate change, and illegal harvesting. This study explores how elevation and population size influence key-life history traits of D. rotundifolia, such as flowering phenology, prey capture efficiency, seed production, and germination rates. Higher elevations and larger populations are associated with extended flowering durations and increased prey capture. However, seed production and germination rates show complex patterns that are likely influenced by genetic diversity, resource availability, or environmental conditions. These insights inform targeted conservation strategies, emphasizing habitat preservation, enhancing prey availability, and maintaining genetic diversity. Public education and community engagement …

An Update On Novel Taxa And Revised Taxonomic Status Of Bacteria Isolated From Domestic Companion And Agricultural Animals Described In 2023, Sara D. Lawhon, Claire R. Burbick, Trinity Kreuger, Elena Ruiz-Reyes, Erik Munson

Clinical Lab Sciences Faculty Research and Publications

With the proliferation of abundant bacterial genomic data comes the recognition of new organisms as well as a better understanding of the relatedness of known bacteria. Recognizing the associated taxonomic changes enhances communication and understanding about the significance of novel organisms and deeper understanding of known pathogens. This review addresses the addition of multiple gastrointestinal bacteria that form the normal microbiota in a variety of animals including honeybees as well as novel bacteria from domestic animals including an alpha-hemolytic Streptococcus species from guinea pigs, two Moraxella spp. from cows and goats, a new Capnocytophaga species from cats, a thermophilic Campylobacter …

Integrating Responsible And Ethical Conduct Of Research Into Cures: A Longitudinal Study Observing Professional Development Of Fellows Within The Ethics Network For Course-Based Opportunities In Undergraduate Research, Aimee Angelica Hernandez-Gaytan

Open Access Theses & Dissertations

No abstract provided.

Enhancement Of Zika Virus Infection By West Nile And Dengue Virus Antibody Positive Cord-Blood Samples From Mothers In El Paso-Texas, Jeanette Orbegozo

Open Access Theses & Dissertations

The recent Zika virus (ZIKV) outbreak and its association with microcephaly cases and other neurological syndromes are important public health concerns. A phenomenon known as antibody-dependent enhancement (ADE) involves preexisting flavivirus antibodies to a specific flavivirus that enhances the severity of a second infection with a different flavivirus. Since ZIKV is related to flaviviruses like West Nile virus (WNV) and dengue virus (DENV), prior exposure to these viruses could elicit cross-reactive antibodies that may enhance a subsequent ZIKV infection. Therefore, this study aimed to determine if WNV and DENV antibodies from cord blood enhanced ZIKV infection in vitro. Initially, a …

Functional Analysis Of The Zebrafish Orthologs Of Host Cell Factor 1 Gene, David Paz

Open Access Theses & Dissertations

Host Cell Factor C1 (HCFC1) is a transcriptional co-activator factor that regulates the expression of over 5000 different downstream target genes in human cells. Missense mutations in the HCFC1 gene cause methylmalonic acidemia homocysteinemia cblX type (cblX). cblX, a multiple congenital anomaly syndrome (MCA) characterized by abnormal brain development, craniofacial abnormalities, metabolic deficits, and intractable epilepsy. Published studies suggest that HCFC1 regulates neural precursor proliferation, number, and differentiation. However, these results were derived from studies that were performed with nonsense alleles or transient knockdown approaches. Although these results demonstrate the importance of HCFC1 in brain development, they do not replicate …

Functionalized Carbon Quantum Dots: A Promising Nanomaterial For Investigating Their Potential In Neurodegenerative Disease Treatment, Sherin M. El Morsy

Open Access Theses & Dissertations

Neurodegenerative diseases such as Parkinson's Disease (PD), Alzheimer's Disease (AD), and Huntington's Disease (HD) remain incurable, despite extensive research into therapeutic development. In the United States of America, the annual cost of PD and AD in terms of patient care and lost productivity is approximately $200 billion. Consequently, there is a critical and unaddressed need for the development of innovative drugs and drug delivery systems that can decelerate, stop, or ideally reverse neurodegenerative diseases. The dissertation presented here focuses on devising strategies to target PD through multiple mechanisms, including the inhibition of protein aggregation and the reduction of oxidative stress. …

Redefining Approaches For Measuring Landscape Subsidence And Permafrost Degradation In Arctic Tundra Environments, Tabatha Lynn Fuson

Open Access Theses & Dissertations

As climate change accelerates in the Arctic, the degradation of permafrost is leading to significant landscape transformation in tundra landscapes. This dissertation investigates the multifaceted responses of permafrost systems to warming, focusing on the dynamics of surface elevation changes and active layer thickness (ALT) across the North Slope of Alaska. In this study, I explore the capacity of repeat Terrestrial Laser Scanning (TLS) technology for modeling tundra features and detecting surface subsidence, specifically how different climate and landscape conditions during scanning impact TLS model precision. We also compare TLS model precision estimates to TLS model accuracy by comparing elevation values …

Environmental Change As A Driver Of The Evolution Of Migratory Behavior In Suboscine Birds, Emily Johns

Open Access Theses & Dissertations

Avian migration, particularly long-distance seasonal migratory movement, has long captured the attention of researchers and the public alike. However, the evolutionary origins of migratory behavior are poorly understood, in part due to limited understanding of the full range of migratory strategies including short-distance and intra-tropical movements. We examine the climatic drivers of migration across suboscine birds, a group of roughly 1,300 primarily Neotropical species with a complex array of migratory strategies. Our reconstructions of the evolutionary history of suboscine migration reveal that migratory behavior is labile and is lost approximately twice as often as it is gained. We find that …

The Influence Of Pitch Type (Fastball, Curveball, And Change-Up) On The Contralateral Trunk Tilt In Adolescent Pitchers, Arlene Nichole Perez

Open Access Theses & Dissertations

Baseball participation has grown, with over 15.59 million players aged six and older in 2021. Pitching relies on effective coordination of the kinetic chain to transfer forces and generate ball velocity. While trunk rotation during pitching has been studied, contralateral trunk tilt across pitch types remains underexplored. This study investigated differences in hand velocity, stride length, and contralateral trunk tilt between fastball, curveball, and change-up pitches in 16 adolescent pitchers ages 15-18. Motion data were collected using a seven-camera system interfaced with Theia 3D. Repeated measures ANOVA revealed a significant difference in hand velocity between fastball and change-up (p = …

Development Of A Novel Autoantibody Panel For Prostate Cancer Detection Based On Immunoproteomic Profiling And Cancer Driver Genes, Cuipeng Qiu

Open Access Theses & Dissertations

Prostate cancer (PCa) is the second most frequent cancer in males with 1.47 million new cases and 396,700 deaths worldwide in 2022. It is the second leading cause of cancer-related deaths among males in the United States with over 35,000 estimated deaths in 2024. PCa is a type of indolent cancer that can progress to an advanced metastatic castration-resistant (mCRPC) stage if not detected and treated early. Screening precancerous lesions or early tumor detection is an effective strategy to reduce the mortality of cancer. Despite the development of effective screening methods, such as the prostate-specific antigen (PSA) test, the specificity …

Novel Therapuetic Approaches For Chagas Disease, Juan Carlos Silva

Open Access Theses & Dissertations

Chagas disease (CD) is caused by the hemoflagellate protozoan Trypanosoma cruzi. This disease is considered one of the 20 neglected tropical diseases (NTD), and it is estimated that 6 to 8 million people worldwide are diagnosed with CD. In the United States, Chagas infection is not reported in all states; however, most reported CD cases are chronic infections. Chagas infection is characterized by two phases: an acute and a chronic phase. Until this present moment, no prophylactic or therapeutic human vaccine is available. However, in a recent study in a murine model, our group showed that the synthetic 20-merpeptide (DAENPGGEVFNDNKKGLSRV) …

Analysis Of Microbiome In Various Human Medical Conditions Using Next-Generation Sequencing Of The 16s Rrna Gene, Pallavi Sharma

Electronic Theses and Dissertations

Studies of human microbiomics, spanning diverse anatomical sites, offer a unique perspective into the complex microbial communities within the body. Influenced by age, gender, and disease, it mirrors broader microbial ecosystems. This study specifically delves into the ocular and gastrointestinal tract microbiome, comparing its composition in 43 healthy and diseased patients using 16S rRNA gene sequencing. We identified a rich microbial landscape, identifying over 338 bacterial taxa. Notably, Acinetobacter, Cutibacterium, Acidovorax and Herbaspirillum were prevalent in the ocular samples and Akkermansia, Faecalibacterium, and Anaerostipes were mostly prevalent in gut samples. Moreover, dry eye disease conditions were characterized by …

Neural Network Architecture Search Enabled Wide-Deep Learning (Nas-Wd) Integrated Hyperspectral Imaging Understanding For Woody Breast In Poultry Processing, Chaitanya Kumar Reddy Pallerla

Graduate Theses and Dissertations

The development and implementation of a Wide & Deep (WD) learning model tailored for classification and regression tasks utilizing spectral data provides a robust solution to evaluate woody breast (WB) conditions in poultry fillets. This process begins with thorough data preprocessing, which includes loading spectral and classification datasets, imputing missing values with medians, and splitting the data into training and testing sets to ensure rigorous model evaluation. The WD model architecture integrates wide linear models and deep neural networks to harness the strengths of both approaches. The wide component excels at memorizing sparse feature interactions, while the deep component captures …

Investigating Consumer Perception And Wine Quality Impact On Alternative Packaging On Grape (Vitis), Mark Walker Bartz

Graduate Theses and Dissertations

The grape (Vitis species) and wine industry has major global economic impacts but faces issues with sustainability. The use of glass bottles for wine production is environmentally unsustainable due to energy-intensive manufacturing and poor recycling rates. However, wine consumers tend to associate glass bottles with higher quality over alternative wine packaging materials such as aluminum, polyethylene terephthalate (PET), and flexible bags/pouches. Since wine price and packaging influence consumers perception of wine quality, wine producers need to understand consumers to maximize profits. Thus, the overall objectives of this research were to investigate consumer perception and wine quality impact of alternative …

Enhancing Selection Intensity With High-Throughput Phenotyping Data In Plant Breeding, Ashmita Upadhyay

Graduate Theses and Dissertations

Genomic prediction (GP) has proven to be an essential tool to accelerate the cultivar development pipeline by predicting the performance of un-phenotyped lines in the field of plant breeding. However, the prediction accuracy is bounded by the heritability of the target trait, limiting the efficiency of the single-trait GP model. To overcome the limitation, a multi-trait genomic prediction model using high-throughput phenotyping (HTP) data can be used to leverage secondary traits to boost the predictive ability of the target trait. This study aimed to assess the efficiency of the multi-trait (MT)- GP model powered by HTP derived secondary traits in …

Assessing The Influence Of Native And Invasive Grasses On The Behavior Of Aedes Aegypti In South Texas, Emily Medelez

Theses and Dissertations

Invasive grasses in South Texas can have widespread negative effects, displacing native species, reducing biodiversity, and posing risks to public health. These grasses may flourish in moist, shaded environments, which not only support their growth but also creates habitats favorable for mosquito activity. This can increase the population and alter the behavior of mosquitoes, potentially raising the risk of disease transmission. In this thesis, I look at the behavior of mosquito species Aedes aegypti, primary vector of the yellow fever virus. I analyzed the influence of oviposition, flight and resting behaviors. I tested the relative attraction of Aedes mosquitoes …

Using High-Pressure Processing To Improve The Quality Of Dark-Cutting Beef, Ashley A. Hahn

Department of Animal Science: Dissertations, Theses, and Student Research

Previous studies have shown high-pressure processing (HPP) can be utilized to improve lean color of dark-cutting beef color by increasing the lightness and redness of steaks. However, the optimal parameters of length of time product is held under pressure and at which pressure must be applied have yet to be fully defined. Furthermore, the impact of HPP on color labile muscles in dark-cutting beef are currently unknown. Therefore, two studies were designed and specific efforts were focused on first defining optimal parameters for color stable muscles, followed by determining the effect of HPP on dark-cutting color labile muscles.

In the …

Relationship Between Host Genomics And Microbiomics In Beef Cattle, Andrew D. Lakamp

Department of Animal Science: Dissertations, Theses, and Student Research

As sequencing technology becomes more affordable and throughput increases, microbiome information is becoming more readily available. For beef cattle selection, microbial information has a variety of uses including being a target for genetic prediction or used as a means to explicitly describe additional phenotypic variability in other traits.

Infectious bovine keratoconjunctivitis (IBK), commonly known as pinkeye, is a disease that infects the ocular surface and surrounding tissue which is an animal health and producer economic concern. Vaccinations have shown to have variable effectiveness, while limited genetics studies have suggested that direct genetic selection for resistance would be slow. Therefore, an …