Life Sciences Commons^™

Understanding The Impact Of Physicochemical Modifications On The Cold Gelling Behavior Of Micellar Casein Concentrate Dispersions, Nathan Pougher

All Graduate Theses and Dissertations, Fall 2023 to Present

When skim milk is filtered via microfiltration, the amount of casein (one of the major milk proteins) in solution can be concentrated. When casein content is high enough (>15%), the solution forms a gel at cold temperatures. With growing trends in the food industry towards simplistic ingredient labels, commonly used gums and stabilizers in the dairy industry are becoming less preferred. In the future, there is potential for the gelling properties of micellar casein to be applied to dairy products as a thickener or stabilizer, but the mechanism behind gel formation isn’t understood well. In this study, the gel …

Atmospheric Mercury At Storm Peak Laboratory: Development Of Methods To Calibrate Ambient Oxidized Mercury Measurements And Comparisons To A 3-D Photochemical Transport Model, Tyler Raymond Elgiar

All Graduate Theses and Dissertations, Fall 2023 to Present

Mercury (Hg) is a highly toxic metal found ubiquitously throughout the environment that has the potential to harm wildlife and human health. Human exposure to Hg can lead to symptoms such as blindness, loss of memory, ataxia, and death. Hg exposure to pregnant women and young children is of even greater concern, as high levels of Hg can hinder fetal and child development.

Hg is emitted into the atmosphere through natural and anthropogenic processes such as biomass burning and the incineration of coal, respectively. Hg exists in the atmosphere as elemental Hg (Hg⁰) or in an oxidized form …

Balsam Woolly Adelgid And Host Forest Characteristics: Impacts And Interactions In Recently Invaded Areas Of Northern Utah And Southeastern Idaho, Grayson B. Jordan

All Graduate Theses and Dissertations, Fall 2023 to Present

The balsam woolly adelgid (BWA), is an invasive forest insect native to central and southern Europe. In 2017, it was detected in Utah for the first time. While BWA had limited impact as a pest in its native European range, it has caused considerable damage to true fir populations in North America. In the Intermountain region, subalpine fir has been identified as the tree species most at risk of BWA infestation. Subalpine fir provides a variety of ecosystem services and is a critical component of the spruce-fir alpine forests of the area. With an expected increase in the severity and …

Dusky Grouse Population Ecology And Thermal Landscape Ecology In The Great Basin Ecosystem, Stephanie M. Landry

All Graduate Theses and Dissertations, Fall 2023 to Present

Dusky Grouse are a mountainous forest grouse found throughout the western, inland mountain ranges of the United States and Canada. While a few studies have looked at Dusky Grouse in the Rocky Mountain Ecosystem of their range, there have been no prior studies of the Dusky Grouse in the Great Basin Ecosystem aside from a brief survey by Zwickel and Bendell in 2004 in the Duck Creek Range of Nevada. With the available habitats differing in both species diversity and availability on the landscape between the two Ecosystems, I wanted to assess characteristics about the Dusky Grouse populations at the …

Understanding The Pizza Baking Properties Of Low Moisture Part Skim (Lmps) Mozzarella Cheese, Anjali Verma

All Graduate Theses and Dissertations, Fall 2023 to Present

Mozzarella cheese is expected to melt, stretch, release free oil and exhibit browning and blistering when baked on pizza. This research aimed to investigate the effects of different starter cultures (based on lactose and galactose utilizing ability) and calcium levels on the functionality of Mozzarella cheese when baked on pizza. Four combinations of starter cultures using S. thermophilus (STI 06)-control, Lac⁺ Gal⁺ L. helveticus (LH 32), and Lac^- Gal⁺ adjunct culture L. helveticus (LH 7995) were used to make the cheese samples. Various tests were conducted over a seven-week period to evaluate their baking properties. The …

Anthropogenic Effects On Global Soil Nitrogen Pools, Shih Chieh Chien, Jennifer Adams Krumins

Department of Biology Faculty Scholarship and Creative Works

The amount of nitrogen stored in terrestrial soils, its “nitrogen pool”, moderates biogeochemical cycling affecting primary productivity, nitrogen pollution and even carbon budgets. The soil nitrogen pools and the transformation of nitrogen forms within them are heavily influenced by environmental factors including anthropogenic activities. However, our understanding of the global distribution of soil nitrogen with respect to anthropogenic activity and human land use remains unclear. We constructed a meta-analysis from a global sampling, in which we compare soil total nitrogen pools and the driving mechanisms affecting each pool across three major classifications of human land use: natural, agricultural, and urban. …

P-Ring: The Conserved Nature Of Phosphorus Enriched Cells In Seedling Roots Of Distantly Related Species, Aniruddha Acharya, Thomas C. Pesacreta

Faculty Publications - Biological Sciences

Plants require sunlight, carbon dioxide, water and mineral ions for their growth and development. Roots in vascular plants sequester water and ions from soil and transport them to the aboveground parts of the plant. Due to heterogeneous nature of soil, roots have evolved several regulatory barriers from molecular to organismic level that selectively allows certain ions to enter the vascular tissues for transport according to the physiological and metabolic demands of plant cell. Current literature profusely elaborates about apoplastic barriers, but the possibility of the existence of a symplastic regulation through phosphorous-enriched cells has not been mentioned. Recent investigations on …

Cell Aggregation Activates Small Gtpase Rac1 And Induces Cd44 Cleavage By Maintaining Lipid Raft Integrity, Dong Li, Younhee Park, Hami Hemati, Xia Liu

Markey Cancer Center Faculty Publications

Lipid rafts are highly ordered membrane domains that are enriched in cholesterol and glycosphingolipids and serve as major platforms for signal transduction. Cell detachment from the extracellular matrix (ECM) triggers lipid raft disruption and anoikis, which is a barrier for cancer cells to metastasize. Compared to single circulating tumor cells (CTCs), our recent studies have demonstrated that CD44-mediatd cell aggregation enhances the stemness, survival and metastatic ability of aggregated cells. Here, we investigated whether and how lipid rafts are involved in CD44-mediated cell aggregation. We found that cell detachment, which mimics the condition when tumor cells detach from the ECM …

High Throughput Phenotyping: Field Based Triticale Breeding And Educational Resource Impact, Catherine Kay Mick

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Triticale (Triticosecale) is a multifunctional hybrid cereal crop that adopted the hardiness of rye and wheat's high-yielding and nutritional qualities. Plant breeding programs work to improve the quality and number of varieties available to producers through multiple rounds of evaluation and selection. However, traditional phenotyping methods are labor-intensive, time-consuming, and destructive, creating a phenotyping bottleneck. Remote sensing using unmanned aerial systems has the potential to alleviate this issue and change the evaluation of phenotypes in a breeding. Demand for educational resources to advance public awareness and prepare the workforce has increased with the utilization of more technology in …

Vulnerabilities Of Greater Prairie Chicken And Tier 1 At-Risk Species In Nebraska Caused By Grassland Transition To Woody Dominance, Robert Peterson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Woody plant encroachment is a global threat and has been transitioning grasslands to woody dominance at a biome scale. This threat is present in the Great Plains grassland biome which is currently experiencing grassland biome collapse as the alternative woody biome advances northwest. Nebraska, which contains the most intact temperate grassland in the world, is currently at the front lines of this large-scale transition making this state’s management decisions vital for the remaining grasslands and the species which rely on these ecosystems. In this study, we assess the vulnerability of Greater Prairie Chicken and Tier 1 at-risk species in Nebraska …

Evaluation Of Vegetative Indices To Determine Canopy Ground Cover For Winter Survival And Hybrid Necrosis In Winter Wheat, Micheal Young

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The benefit of unmanned aircraft systems and image processing methods in agronomic research across numerous crops has been well documented as has the importance of wheat, Triticum aestivum L., on the global food supply. Hence there is great interest in digital solutions applied to aspects of wheat breeding. A major trait of importance to winter wheat breeders in higher latitudes is winter survival, which can result in poor yield and performance if lines do not survive extreme cold. Scoring winter survival is most commonly based on visual score of 0% to 100% with the higher percentage conveying higher winter survival …

Unraveling The Tapestry Of Indigenous Maize In North America: A Case Study Of Pawnee Ancestral Maize, Kahheetah Barnoskie

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Studies on Indigenous ancestral landrace maize in North America has significant historical and scientific importance. Indigenous peoples, such as the Pawnee people, have been cultivating maize for thousands of years, resulting in diverse varieties adapted to their local environments. This study aims to deepen the knowledge of Indigenous maize by examining specific varieties from the Pawnee, including a comparative analysis of the genetic makeup through DNA sequencing. This study used Genotyping by Target Sequencing (GBTS) method to examine the genetic variation and characteristics among the multiple varieties the Pawnee people once grew historically, providing valuable information about the evolutionary history …

Empirical Evidence Supporting The Inclusion Of Multi-Axes Segregation In Assessing Us County Health, David W. S. Wong, Debasree Das Gupta

Kinesiology and Health Science Faculty Publications

To facilitate community action toward health equity, the County Health Rankings & Roadmaps program (CHR&R) assigns health rankings to US counties. The CHR&R conceptual model considers White-Black and White-non-White dissimilarity values to represent residential segregation as part of the family and social support subcomponent. As the US is greying and becoming more multi-racial-ethnic, the two-group White-centered segregation measures are inadequate to capture segregation among population subgroups in the US. Thus, we evaluate the relevancy of segregation measures that consider multiple racial, ethnic, and age groups in assessing US county health. Besides using the two-group dissimilarity index to measure Whitecentered racial …

Investigations Of The Impact Of Excess Gut Volume On Spinal Angles, Carly Stoops

Honors Projects

This compilation of research projects investigates the impact of increased gut volume on the lumbosacral disc angle and the central lumbar angle of the spine. The magnitudes of the lumbosacral disc angle can change due to age, pregnancy, sex, and, as hypothesized, gut volume. It is unknown how the central lumbar angle changes due to increased gut volume. This is important because these spinal modifications can result in pain, specifically in the lower back. The consequences of being overweight or obese rarely include the anatomical changes that result due to excess gut volume and center of mass changes. My goal …

Using Plastinated Cadaveric Models For Anatomical Study Of Dorsalis Pedis Artery And Its Variation, Steven Bolton, Ifunanya Monube, Hooman Tabatabai-Mir

School of Podiatric Medicine - Student Research

The dorsalis pedis artery is a main artery of the dorsum of the foot. It directly arises from the anterior tibial artery and travels distally to the base of the 1st metatarsal. Its main function includes supplying oxygenated blood to the dorsal surface of the foot. A weak dorsalis pedis pulse is an indication of underlying circulation issue, such as peripheral vascular disease. Anatomical variations of the artery are not uncommon, including the total absence of the artery. Branching variations occur in approximately 10% of the population. Knowledge of these variations is critical for surgeons to prevent surgical complications

Thawing And Storage Effects On Quality Of Plant-Based Sausage Patties, Dakota Cook

All Theses

Maintaining sausage analogue quality during a thawing process and storage is important for increasing consumer satisfaction. Following new trends and prevalence of plant-based meats and a flexitarian diet, two experiments were conducted to test how thawing methods (experiment 1) and storage temperatures (experiment 2) affect meat analogue quality. For Experiment 1, Jimmy Dean Original Sausage Patties Made with Pork & Turkey, Morningstar Veggie Original Patties and Beyond Sausage Original Patties were purchased pre-frozen and subsequently thawed in one of three ways: 1. thawed at 4°C for 24 hours, 2. thawed at room temperature (22°C±2°C) for 4 hours and 3. thawed …

Effect Of Storage Temperatures And Home Refrigeration Systems On The Shelf Life Of Frozen Desserts, Natalie Sinclair

All Theses

The effect of storage temperature over 12 weeks was studied using dairy and non-dairy frozen desserts. Research was conducted to determine if higher freezer temperatures affect frozen dessert quality to reduce energy use associated with lower freezer temperatures. Samples were analyzed for ice crystal pore size using micrographs, grittiness by a trained sensory panel and weight loss during storage. Ice crystal size was determined using scanning electron microscopy and grittiness was measured on a 5-point ranking scale, with 1 being least gritty and 5 being most gritty. Weight loss was determined by measuring sample weights before and after the 12-week …

The Chemical And Physical Analysis Of Hiveless Tm A Plant Based Honey Alternative, Javin Goodine

All Theses

Honey is perhaps the oldest and one of the world's most widely used natural sweeteners. It is a viscous, sweet, golden substance bees produce via natural enzymatic and physical modification of flower nectar. It has long been prized for its unique flavor and medicinal properties. It can be incorporated into many applications, such as bread, cured meats, alcohol, condiments, candy, pharmaceuticals, and skin care products. In 2022 alone, America consumed over 600 million pounds of honey (Board Reports, 2023), but less than a fifth of that was produced domestically, the rest imported from other countries. The international honey trade can …

A Genome Wide Association Study Of Heat Tolerance In Snap Bean (Phaseolus Vulgaris), Morgan Stone

All Theses

Bean production in the United States has decreased while the temperatures have been steadily increasing, reaching new highs each year. Heat stress is detrimental to common bean (also known as snap bean) production. Symptoms of heat stress include decrease in pollen viability, shriveling of pods, and pod abortion making them unmarketable. Pod production of 323 snap bean accessions from a large diversity panel was assessed in a randomized complete block design with field trials at two different times in the spring season. The results show a significant decrease in the number of pods produced per plant and weight of pods …

The Floral Thermal Environment: Physical, Morphological, And Environmental Determinants, And Their Impact On Plant-Pollinator Interactions, Jennifer Apland

All Theses

Anthropogenic climate change is driving major shifts in global temperatures and increases in extreme temperature events that contribute to reduced survival and species loss. To counteract extreme temperatures, many organisms can undergo geographic range shifts or engage in behavioral thermoregulation (e.g., movement to suitable microhabitats). While plants are sessile and thus subject to highly variable ambient temperatures, they have evolved mechanisms to regulate internal floral temperature. Floral thermoregulation may mitigate thermal stress on pollen and ovules and impact plant-pollinator interactions. These mechanisms for thermoregulation are often highly dependent on ambient temperature and solar radiation as most plants are not endothermic. …

Developing Rapid Indicators To Detect Micropollutants In Coastal Blackwater Rivers In South Carolina, Lydia Winn

All Theses

Micropollutants in water sources are found in higher concentrations in areas with high levels of urban and agricultural land. These pollutants can be introduced into water sources during storms and rain events, through water treatment outputs, and commercial or residential waste. These events increase the levels of both nutrients and micropollutants in these water sources. With increasing levels of micropollutants in rivers, this study’s goal was to develop an alternative detection method or an indicator test for the levels of micropollutants in water. We first proposed the use of amicrobial fuel cell (MFCs) operating as a biosensor could be utilized …

The Effects Of Sous Vide Cooking And Cysteine Proteases On Instrumental And Sensory Textural Properties Of Lower Value Cuts Of Beef, Ian Smith

All Theses

Sous vide cooking has the potential to increase the value of lower quality cuts of beef. Understanding the textural properties following a cook-chill sous vide process is crucial to maximizing this potential. The use of enzymes may further increase the value of these products if able to positively alter the texture of the final product.

The first research objective was to use instrumental texture analysis to evaluate potential relationships between enzymatic treatments and sous vide processing time of lower value cuts of beef. Three cuts were evaluated: infraspinatus (top blade), semitendinosus (eye of round), and beef tongue. Infraspinatus and semitendinosus …

Rapid Leaf Senescence Symptoms Are Related To Carbohydrate Depletion In Cut Chrysanthemums, And Strategies For The Symptoms Reduction, Shara Ortiz Carvajal

All Theses

Chrysanthemum (Chrysanthemum ×morifolium) is the second-largest exported cut flower worldwide; however, some cultivars exhibit rapid leaf senescence during their first week of vase life. This phenomenon negatively impacts consumer perception of plant quality, and its cause has been unknown. Experiments were performed in Colombia on cut chrysanthemums shipped to the U.S. for vase-life evaluation. After 10 d, the severity of leaf senescence symptoms was recorded. Experiments examined the effect of flower form [disbud (one flower per stem) versus spray (5-10 flowers per stem)], the effect of time of harvest (A.M. versus P.M.), and sugar sources (dextrose, fructose, mannitol, and sucrose) …

Germination Trends Of American Chaffseed, Schwalbea Americana L., And Factors Affecting First-Year Seedling Development, Trenton Miller

All Theses

Following centuries of exploitation and fire suppression, longleaf pine systems are now the focus of many conservation efforts. Efforts to restore populations of Schwalbea americana L. in longleaf pine savannas have been met with frustratingly low recruitment. While past studies have briefly quantified germination rates for Schwalbea, there have not been any studies yet that truly investigate this plant’s germination requirements. Additionally, there has been little research into characterizing the parasitic relationship between Schwalbea and its various host species. We conducted a germination study in a growth chamber that investigated Schwalbea’s germination rate and time to germinates as …

Cover Crops Integration With Nitrogen Management For Dryland Cotton In Sandy Ultisols, Swabir Alhassan Musah

All Theses

One of the many benefits of cover crops (CCs) is nutrient provision, which can be optimized by synchronizing the nutrient release from residue decomposition with the demand for subsequent cash crops. This study is part of a multistate research (13 states) to measure cotton lint yield in response to cover cropping as a nutrient management strategy. This research-based information will help farmers optimize nitrogen (N) fertilization when integrating CCs into their production systems. A two-year field trial was conducted in 2021 and 2022 with four cover crop treatments (cereal rye, crimson clover, mixtures of cereal rye and crimson clover, and …

Logging In The Southeastern Us: Facing Challenges, Finding Opportunities, Shubhechchha Khadka

All Theses

The logging businesses, a crucial component of the wood supply chain, face shifting dynamics with various challenges and opportunities. Focusing on North and South Carolina, this study analyzes surveys and interviews to explore the logging business challenges and pathways to sustainable growth in the Southeastern United States.

In South Carolina, our findings indicate a shift towards more medium-sized businesses with a decrease in small and large logger, the entry of some younger loggers in the industry, and a notable rise in educational qualifications among loggers. Simultaneously, there is a decrease in the intergenerational transfer of logging businesses, coupled with escalated …

Novel Protected Gelatin Capsules Containing Fish Oil Mitigated The Effect Of Milk Fat Depression And Reduced Rumen Degradation Compared To Untreated Capsules, Omar Manuel Pena Pena

All Theses

The objective of this study was to assess the effects of feeding gelatin capsules treated with alcoholic solutions of flavoring agents followed by drying containing fish oil on lactation performance, rumen fatty acids content and milk enrichment of fatty acids. In Trial 1, four multiparous ruminally-fistulated Holstein cows were randomly assigned to one of four dietary treatment sequences in a 4 x 4 Latin square design. Treatments consisted of 1) Control with no capsules, 2) Control plus 200 untreated capsules per cow/day, mixed with the TMR; 3) Control plus 200 treated capsules per cow/day placed directly into the rumen, 4) …

Effects Of Seasonal Environment On Abundance, Behavior, Visual Signaling, And Perception In Prairie Butterflies, Grace Elizabeth Hirzel

Graduate Theses and Dissertations

Phenotypic plasticity is a widespread mechanism that allows animals to respond quickly to seasonal changes in their environment. However, the depth and complexity of these plastic responses is still being discovered. One aspect of seasonal physiology that is relatively unstudied is changes to sensory physiology and how it may explain changes in behavior. To determine how changes in seasonal conditions affect behavior, coloration, and vision, I examined populations of wild butterflies in Northwest Arkansas. Focusing on a single species, the common buckeye Junonia coenia, I found that seasonal coloration covaries with thermoregulating behavior and is accompanied by changes in visual …

Demographics, Sexual Dimorphism, And Ecological Aspects Of Ambystoma Annulatum (Ringed Salamander) In Northwest Arkansas, Usa, Brian M. Becker

Graduate Theses and Dissertations

The need to study and monitor amphibian populations is increasing along with the threats to their population stability and persistence in nature. Northwest Arkansas is one of the fastest growing areas in the nation and with that growth comes rapid changes in land use, massive alterations to habitats, habitat loss, and the introduction of nonnative plants and animals. Ambystoma annulatum (Ringed Salamander) is an Ambystomatid endemic to the Ozark and Ouachita Mountains of southern Missouri, northern and western Arkansas, and eastern Oklahoma giving it a relatively small distribution compared to most Ambystoma. Therefore, Arkansas constitutes a significant portion of the …

Evaluation Of Oxyfluorfen For Use In Roxy Rice, Casey Hunter Arnold

Graduate Theses and Dissertations

Mid-southern U.S. rice (Oryza sativa L.) producers are challenged every year to control problematic barnyardgrass [Echinochloa crus-galli (L.) Beauv.] and weedy rice (Oryza sativa L.) because they have evolved resistance to most labeled herbicides. Oxyfluorfen is a Herbicide Resistance Action Committee (HRAC)/Weed Science Society of America (WSSA) Group 14 herbicide that inhibits protoporphyrinogen oxidase within the plant. Currently, there are no HRAC/WSSA Group 14 herbicides labeled for barnyardgrass or weedy rice control in midsouthern rice production. Therefore, oxyfluorfen is being evaluated for barnyardgrass and weedy rice control. Plant breeders at the University of California – Davis were able to breed …