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2023

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Full-Text Articles in Life Sciences

Human Dimensions Of Woody Encroachment Management In Nebraska, Emily Rowen Dec 2023

Human Dimensions Of Woody Encroachment Management In Nebraska, Emily Rowen

School of Natural Resources: Dissertations, Theses, and Student Research

Woody plant encroachment (WPE) is a social-ecological problem that will challenge conservation professionals and agricultural producers to adapt their management strategies. This research first examined WPE from the perspective of individual conservation professionals through an online survey. Conservation professionals’ attitudes about adaptation to vegetation transitions, such as WPE, were of interest because these attitudes are one measure of how prepared this group is to respond to WPE. Hypothesized predictors of adaptation attitude were tested through linear regression modeling. These predictors included ecological change, observation of WPE, or risk perception. It was found that risk perception was the strongest predictor of …


The Influence Of Invasive Species On Fishers’ Satisfactions, Caroline M. Laplante Dec 2023

The Influence Of Invasive Species On Fishers’ Satisfactions, Caroline M. Laplante

School of Natural Resources: Dissertations, Theses, and Student Research

Invasives species are prevalent and widespread in North America. Outdoor recreational activities, such as fishing, introduce a point in which humans may interact with invasive species and have to adapt their own behaviors. Bigheaded carp in the Missouri River below Gavin’s Point Dam are a group of invasive fish species that were thought to be negatively relating to recreational fishers’ satisfactions. Using a content analysis and an importance-grid, we conclude that invasive species do not strongly relate to recreational paddlefish fishers’ satisfactions. Paddlefish fishers represent a small sub-set of recreational fishers in Nebraska and South Dakota. The content analysis revealed …


Understanding Avidities Of Recreational Activities For People Possessing Fishing Licenses And Residing In Urban Environments, Kyle F. Hansen Dec 2023

Understanding Avidities Of Recreational Activities For People Possessing Fishing Licenses And Residing In Urban Environments, Kyle F. Hansen

School of Natural Resources: Dissertations, Theses, and Student Research

Recreational fishing is one of the world's most popular pastimes, wherein participation is associated with sociodemographic factors. Even so, fishing license sales are declining in the USA in conjunction with a reduction in rural populations as people move to urban areas. Thus, urban areas are constantly growing in population size, population diversity, and geographic size suggesting a need to understand fishing participation in these growing areas. Natural resource managers often use participation to understand recreationists, yet avidity could provide a new way to understand recreationists. The goal of our study is to understand what sociodemographic factors influence the fishing avidity …


Game-Theoretic Approaches To Optimal Resource Allocation And Defense Strategies In Herbaceous Plants, Molly R. Creagar Dec 2023

Game-Theoretic Approaches To Optimal Resource Allocation And Defense Strategies In Herbaceous Plants, Molly R. Creagar

Department of Mathematics: Dissertations, Theses, and Student Research

Empirical evidence suggests that the attractiveness of a plant to herbivores can be affected by the investment in defense by neighboring plants, as well as investment in defense by the focal plant. Thus, allocation to defense may not only be influenced by the frequency and intensity of herbivory but also by defense strategies employed by other plants in the environment. We incorporate a neighborhood defense effect by applying spatial evolutionary game theory to optimal resource allocation in plants where cooperators are plants investing in defense and defectors are plants that do not. We use a stochastic dynamic programming model, along …


Postural Control Behavior In A Virtual Moving Room Paradigm, Hannah R. Freeman, Harish Chander, Sachini N.K. Kodithuwakku Arachchige, Alana J. Turner, J. Adam Jones, Zhujun Pan, Christopher Hudson, Adam C. Knight Dec 2023

Postural Control Behavior In A Virtual Moving Room Paradigm, Hannah R. Freeman, Harish Chander, Sachini N.K. Kodithuwakku Arachchige, Alana J. Turner, J. Adam Jones, Zhujun Pan, Christopher Hudson, Adam C. Knight

Kinesiology

Background: Taking inspiration from the classical 1974, “moving room experiment” by Lee and Aronson, a “virtual moving room paradigm (Vroom)” was designed using virtual reality (VR) to assess postural control behavior. Methods: Thirty healthy adults (age: 21 ± 1 years; height: 166.5 ± 7.3 cm; mass: 71.7 ± 16.2 kg) were tested for postural stability in a virtual moving room paradigm (Vroom). The Vroom consisted of randomized virtual and visual perturbations of the virtual room moving toward and away from the individual, during both unexpected and expected trials. Objective postural sway variables and subjective experiences to VR using the simulator …


Analyses Of Mybl1 In Triple Negative Breast Cells And Validation Of Genes Colocalized To Chromosome 8q Loci, La Tajia D. Thirston Dec 2023

Analyses Of Mybl1 In Triple Negative Breast Cells And Validation Of Genes Colocalized To Chromosome 8q Loci, La Tajia D. Thirston

Theses (2016-Present)

Fifteen percent of breast cancer are characterized as the triple negative breast cancer subtype. The cancers are recognized as negative for estrogen, progesterone and ErRB2 receptors, genes that function as transcription and growth factor functions, respectively. Previous data show the MYBL1 transcription factor is over-expressed in some triple negative breast cancers-suggesting dysregulation of the gene in the cancers. MYBL1 gene is a strong transcriptional regulator associated with regulation of cell cycle proliferation which is a key event in cancer progression. To determine genes either directly or indirectly associating with MYBL1 in these cancers we knocked the gene down, performed microarray …


Amyloid Fibrils Of Human Fgf-1 Induced By Different Detergents, Zeina Ismael Ibrahem Alraawi Dec 2023

Amyloid Fibrils Of Human Fgf-1 Induced By Different Detergents, Zeina Ismael Ibrahem Alraawi

Graduate Theses and Dissertations

Nature achieves molecular self-assembly through the ordered growth of nanoscale building blocks with high efficiency to fabricate macromolecular architectures. One example of self- assembly is peptides folding onto protein is one of the most astounding biological self-assembly processes. When proteins aggregate to form amyloid fibers, the secondary structure of the protein converts from its native state to a cross-beta-sheet. Fibroblast growth factors (FGFs) possess an essential role in neuronal survival during development. In addition, they are involved in neural stem cell (NSC) proliferation. Fibroblast growth factors (FGFs) are well known to be synthesized in the central nervous system (CNS) and …


Global Dataset Of Soil Organic Carbon In Tidal Marshes, Tania L. Maxwell, André S. Rovai, Maria F. Adame, Janine B. Adams, José Álvarez-Rogel, William E. N. Austin, Kim Beasy, Francesco Boscutti, Michael E. Böttcher, Tjeerd J. Bouma, Richard H. Bulmer, Annette Burden, Shannon A. Burke, Saritta Camacho, Doongar R. Chaudhary, Gail L. Chmura, Margareth Copertino, Grace M. Cott, Christopher Craft, John Day, Carmen B. De Los Santos, Lionel Denis, Weixin Ding, Joanna C. Ellison, Carolyn J. E. Lewis, Luise Giani, Maria Gispert, Swanne Gontharet, José A. González-Pérez, M. Nazaret González-Alcaraz, Connor Gorham, Anna E. L. Graversen, Anthony Grey, Roberta Guerra, Qiang He, James R. Holmquist, Alice R. Jones, José A. Juanes, Brian P. Kelleher, Karen E. Kohfeld, Dorte Krause-Jensen, Anna Lafratta, Paul S. Lavery, Edward A. Laws, Carmen Leiva-Dueñas, Pei S. Loh, Catherine E. Lovelock, Carolyn J. Lundquist, Peter I. Macreadie, Inés Mazarrasa, J. Patrick Megonigal, Joao M. Neto, Juliana Nogueira, Michael J. Osland, Jordi F. Pagès, Nipuni Perera, Eva-Maria Pfeiffer, Thomas Pollmann, Jacqueline L. Raw, María Recio, Ana C. Ruiz-Fernández, Sophie K. Russell, John M. Rybczyk, Marek Sammul, Christian Sanders, Rui Santos, Oscar Serrano, Matthias Siewert, Craig Smeaton, Zhaoliang Song, Carmen Trasar-Cepeda, Robert R. Twilley, Marijn Van De Broek, Stefano Vitti, Livia V. Antisari, Baptiste Voltz, Christy N. Wails, Raymond D. Ward, Melissa Ward, Jaxine Wolfe, Renmin Yang, Sebastian Zubrzycki, Emily Landis, Lindsey Smart, Mark Spalding, Thomas A. Worthington Dec 2023

Global Dataset Of Soil Organic Carbon In Tidal Marshes, Tania L. Maxwell, André S. Rovai, Maria F. Adame, Janine B. Adams, José Álvarez-Rogel, William E. N. Austin, Kim Beasy, Francesco Boscutti, Michael E. Böttcher, Tjeerd J. Bouma, Richard H. Bulmer, Annette Burden, Shannon A. Burke, Saritta Camacho, Doongar R. Chaudhary, Gail L. Chmura, Margareth Copertino, Grace M. Cott, Christopher Craft, John Day, Carmen B. De Los Santos, Lionel Denis, Weixin Ding, Joanna C. Ellison, Carolyn J. E. Lewis, Luise Giani, Maria Gispert, Swanne Gontharet, José A. González-Pérez, M. Nazaret González-Alcaraz, Connor Gorham, Anna E. L. Graversen, Anthony Grey, Roberta Guerra, Qiang He, James R. Holmquist, Alice R. Jones, José A. Juanes, Brian P. Kelleher, Karen E. Kohfeld, Dorte Krause-Jensen, Anna Lafratta, Paul S. Lavery, Edward A. Laws, Carmen Leiva-Dueñas, Pei S. Loh, Catherine E. Lovelock, Carolyn J. Lundquist, Peter I. Macreadie, Inés Mazarrasa, J. Patrick Megonigal, Joao M. Neto, Juliana Nogueira, Michael J. Osland, Jordi F. Pagès, Nipuni Perera, Eva-Maria Pfeiffer, Thomas Pollmann, Jacqueline L. Raw, María Recio, Ana C. Ruiz-Fernández, Sophie K. Russell, John M. Rybczyk, Marek Sammul, Christian Sanders, Rui Santos, Oscar Serrano, Matthias Siewert, Craig Smeaton, Zhaoliang Song, Carmen Trasar-Cepeda, Robert R. Twilley, Marijn Van De Broek, Stefano Vitti, Livia V. Antisari, Baptiste Voltz, Christy N. Wails, Raymond D. Ward, Melissa Ward, Jaxine Wolfe, Renmin Yang, Sebastian Zubrzycki, Emily Landis, Lindsey Smart, Mark Spalding, Thomas A. Worthington

Research outputs 2022 to 2026

Tidal marshes store large amounts of organic carbon in their soils. Field data quantifying soil organic carbon (SOC) stocks provide an important resource for researchers, natural resource managers, and policy-makers working towards the protection, restoration, and valuation of these ecosystems. We collated a global dataset of tidal marsh soil organic carbon (MarSOC) from 99 studies that includes location, soil depth, site name, dry bulk density, SOC, and/or soil organic matter (SOM). The MarSOC dataset includes 17,454 data points from 2,329 unique locations, and 29 countries. We generated a general transfer function for the conversion of SOM to SOC. Using this …


Nutritional-Environmental Trade-Offs In Potato Storage And Processing For A Sustainable Healthy Diet, Aubin Payne, Ebenezer M. Kwofie, Prince Agyemang, Jamie Baum Dec 2023

Nutritional-Environmental Trade-Offs In Potato Storage And Processing For A Sustainable Healthy Diet, Aubin Payne, Ebenezer M. Kwofie, Prince Agyemang, Jamie Baum

Biological and Agricultural Engineering Faculty Publications and Presentations

Over the last decade, poor diets and limited access to nutritious foods have been critical drivers of micronutrient deficiency in human health. However, food fortification at an industrialized scale in developed countries has helped eliminate deficiency-related diseases. In developing countries, fortified foods and biofortified materials have been delivered to nutrient-deficient communities. While these strategies have produced significant and acclaimed results, reports from the Food and Agricultural Organization suggest that over a quarter of the world’s population suffers from micronutrient deficiency. This implies that there are still declines in micronutrients in food products at different nodes along the food value chain …


Serranidae Spp. Neurocranium, Justin Abonce, David Kerstetter Dec 2023

Serranidae Spp. Neurocranium, Justin Abonce, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.


Forecasting Natural Regeneration In The Great Basin With Ecological Theory And Remote Sensing, Andrii Zaiats Dec 2023

Forecasting Natural Regeneration In The Great Basin With Ecological Theory And Remote Sensing, Andrii Zaiats

Boise State University Theses and Dissertations

Natural regeneration is the foundation of resilient ecosystems. Anthropogenic pressures across the globe have caused the decline of ecosystem diversity, distribution, and functional properties essential to global and local communities. An example of the Great Basin ecosystems in the Western United States demonstrates how foundational shrub species declines due to intensified wildfires and other anthropogenic pressures, undermining the resilience across ecological and social-ecological systems. Ecological restoration aims to assist ecosystems in recovery after wildfires. However, restoration efforts in the Great Basin have had limited success in great part due to highly unpredictable rates of natural regeneration and spatially variable ecosystem …


Predicting Flower Stalk Production Of A Native Shrub Using Uav Structure-From-Motion Photogrammetry, Ryan Scott Wickersham Dec 2023

Predicting Flower Stalk Production Of A Native Shrub Using Uav Structure-From-Motion Photogrammetry, Ryan Scott Wickersham

Boise State University Theses and Dissertations

Climate change is threatening rangeland ecosystems, including increasing frequency of extreme weather, wildfire, and drought. Identifying which native plants are likely to be resilient to these ongoing changes is crucial for developing climate-smart restoration plans. Sagebrush is a foundational species across western rangeland, with variation in flower phenology and success that may indicate a pattern of resilience for the shrub and associated species. Analyzing sagebrush resilience through flowering success will help us understand how vulnerable sagebrush populations and post-restoration sites will respond to extreme weather events. We applied high-resolution remotely sensed data to map flower stalk production in sagebrush plants …


Assessing The Effects Of Non-Native Flowering Plants And Anti-Herbivory Plastic (Vexar®) Tubes On Insect Visitation To Native Plants, Emily R. Sun Dec 2023

Assessing The Effects Of Non-Native Flowering Plants And Anti-Herbivory Plastic (Vexar®) Tubes On Insect Visitation To Native Plants, Emily R. Sun

Boise State University Theses and Dissertations

Bees (Hymenoptera: Apoidea) are among the most prominent pollinators of the flowering plants and play an essential role in maintaining ecosystem function. Disruption of plant-pollinator interactions is a growing global concern because of the implications for biodiversity, ecosystem health, and agriculture. The incursion of non-native plants globally is seen to adversely affect native plants and alter plant-pollinator communities. In chapter one, I examined the effects of a non-native weed, Sisymbrium altissimum (tall tumblemustard), on bee visitation to nearby native Sphaeralcea munroana (Munro’s globemallow), including genera-specific differences among bees. The most common bee genera were Andrena, Colletes, Diadasia, Halictus, and …


Development And Validation Of An Improved Sagittal Plane Device For Infant Product Safety Testing, Sarah Marie Goldrod Dec 2023

Development And Validation Of An Improved Sagittal Plane Device For Infant Product Safety Testing, Sarah Marie Goldrod

Boise State University Theses and Dissertations

Infants need constant care and support through the first year of life. To minimize the burden of ongoing attending, caregivers frequently use commercial infant products to enable them to carry out daily tasks without holding the baby. An estimated 11,500 infant injuries involving nursery products were treated in emergency rooms in 2021, and 58 deaths were reported in seated nursery products between 2017 and 2019. Head-neck flexion and hyperextension and trunk flexion negatively influence normal breathing and can contribute to positional asphyxia in infants. The body position of infants lying within seated products is an important consideration for safety. Thus, …


Winter-Time Cover Crop Identification: A Remote Sensing-Based Methodological Framework For New And Rapid Data Generation, Zobaer Ahmed, Lawton L. Nalley, Kristofor R. Brye, V. Steven Green, Michael P. Popp, Aaron Michael Shew, Lawson Connor Dec 2023

Winter-Time Cover Crop Identification: A Remote Sensing-Based Methodological Framework For New And Rapid Data Generation, Zobaer Ahmed, Lawton L. Nalley, Kristofor R. Brye, V. Steven Green, Michael P. Popp, Aaron Michael Shew, Lawson Connor

Environmental Dynamics Faculty Publications and Presentations

Accurately identifying and systematically mapping winter-time cover crops and their phenological characteristics offer significant benefits to agricultural producers and policymakers, as cover crops are one of several potential solutions to climate change mitigation. We present a methodological framework for identifying and mapping the presence of winter-time cover crops at the field level and aggregated to county scales from 2013 to 2019 by using the Google Earth Engine (GEE), a random forest classifier with time series data from Landsat 8, and yearly cover crop training data from the United States Department of Agriculture (USDA)-Natural Resources Conservation Service (NRCS). The methodology was …


Mapping Density And Distribution Of Ptarmigan And Arctic Ground Squirrels Within Gyrfalcon Breeding Territories In Western Alaska, Michaela L. Gustafson Dec 2023

Mapping Density And Distribution Of Ptarmigan And Arctic Ground Squirrels Within Gyrfalcon Breeding Territories In Western Alaska, Michaela L. Gustafson

Boise State University Theses and Dissertations

Shifting climate patterns are modifying Arctic habitats, stressing the need to understand how rapid climate and habitat changes affect Arctic trophic webs. Despite their crucial role in illuminating the impacts of Arctic changes and their ripple effects on predators, like Gyrfalcons, small Arctic primary consumers remain insufficiently studied across their range. To address this gap, I sought to estimate the distribution and abundance of Gyrfalcons' top three prey species (Willow Ptarmigan, Rock Ptarmigan, and Arctic ground squirrels) within Gyrfalcon breeding territories on the Seward Peninsula, Alaska, USA. I also aimed to discern the relationships between different habitats and species’ abundance. …


(R2059) Modeling The Spread Of Coronavirus With Self-Protection And Quarantine Effect, Dileep Sharma, Agraj Tripathi, Ram Naresh Tripathi Dec 2023

(R2059) Modeling The Spread Of Coronavirus With Self-Protection And Quarantine Effect, Dileep Sharma, Agraj Tripathi, Ram Naresh Tripathi

Applications and Applied Mathematics: An International Journal (AAM)

A nonlinear mathematical model to study the effect of transmission dynamics of COVID-19 virus in a population with variable size structure is proposed and analyzed. The model divides the total human population into five subclasses: susceptibles, self-protected susceptibles, infectives, quarantined infectives, and recovered population including a class representing cumulative density of coronavirus in the environmental reservoir. The model exhibits two equilibria, namely, the diseasefree and the endemic equilibrium. Model analysis reveals the global dynamics of the spread of COVID-19 is completely determined by the basic reproduction number. If basic reproduction number is greater than one, the endemic equilibrium is locally …


Developing Disinfection Strategies For Controlling Human Norovirus, Sars-Cov-2, And Clostridioides Difficile Endospores In Long-Term Care Facilities, Jinge Huang Dec 2023

Developing Disinfection Strategies For Controlling Human Norovirus, Sars-Cov-2, And Clostridioides Difficile Endospores In Long-Term Care Facilities, Jinge Huang

All Dissertations

Long-term care facilities (LTCFs) provide an environment favorable for the transmission of three critical human pathogens: human norovirus (HuNoV), severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and Clostridioides difficile. Given residents in LTCFs are susceptible to infections due to their advanced ages and compromised immune systems, effective environmental surface disinfection plays a crucial role in controlling the spread of human pathogens within these settings and, therefore, mitigates the risk of infections caused by these pathogens. This dissertation aimed to assess the efficacy of various types of disinfectants against two HuNoV surrogates [feline calicivirus (FCV) and Tulane virus (TuV)], two …


Impacts Of Disturbance And Nutrient Addition On Coastal Dune Arthropod Communities, Kayla Beitzel Dec 2023

Impacts Of Disturbance And Nutrient Addition On Coastal Dune Arthropod Communities, Kayla Beitzel

Honors Theses

Coastal dune ecosystems are globally distributed ecosystems that occur from the tropics to the poles and serve as the first line of defense during storms. They are critical habitats for a variety of organisms and also play a critical role in stabilizing coastal habitats. These systems face increased pressure via urban development, recreational expansion, and sea-level rise (Newman et al. 2023; Webster et al. 2005). Insects are vital consumers in coastal ecosystems and act as regulators of plant growth. Low-diversity ecosystems, like coastal dune systems, often contain a higher abundance of low-diverse insect consumers (Ripple et al. 2021), which can …


Deficiency Of Acute-Phase Serum Amyloid A Exacerbates Sepsis-Induced Mortality And Lung Injury In Mice, Ailing Ji, Andrea C. Trumbauer, Victoria P. Noffsinger, Luke W. Meredith, Brittany Dong, Qian Wang, Ling Guo, Xiangan Li, Frederick C. De Beer, Nancy R. Webb, Lisa R. Tannock, Marlene E. Starr, Christopher M. Waters, Preetha Shridas Dec 2023

Deficiency Of Acute-Phase Serum Amyloid A Exacerbates Sepsis-Induced Mortality And Lung Injury In Mice, Ailing Ji, Andrea C. Trumbauer, Victoria P. Noffsinger, Luke W. Meredith, Brittany Dong, Qian Wang, Ling Guo, Xiangan Li, Frederick C. De Beer, Nancy R. Webb, Lisa R. Tannock, Marlene E. Starr, Christopher M. Waters, Preetha Shridas

Saha Cardiovascular Research Center Faculty Publications

Serum amyloid A (SAA) is a family of proteins, the plasma levels of which may increase >1000-fold in acute inflammatory states. We investigated the role of SAA in sepsis using mice deficient in all three acute-phase SAA isoforms (SAA-TKO). SAA deficiency significantly increased mortality rates in the three experimental sepsis mouse models: cecal ligation and puncture (CLP), cecal slurry (CS) injection, and lipopolysaccharide (LPS) treatments. SAA-TKO mice had exacerbated lung pathology compared to wild-type (WT) mice after CLP. A bulk RNA sequencing performed on lung tissues excised 24 h after CLP indicated significant enrichment in the expression of genes associated …


Ecohiv-Infected Mice Show No Signs Of Platelet Activation, Hammodah Rawhi Hammodah Alfar, Dominic Ngima Nthenge-Ngumbau, Kathryn E. Saatman, Sidney W. Whiteheart Dec 2023

Ecohiv-Infected Mice Show No Signs Of Platelet Activation, Hammodah Rawhi Hammodah Alfar, Dominic Ngima Nthenge-Ngumbau, Kathryn E. Saatman, Sidney W. Whiteheart

Saha Cardiovascular Research Center Faculty Publications

Platelets express several surface receptors that could interact with different viruses. To understand the mechanisms of HIV-1′s interaction with platelets, we chose the EcoHIV model. While EcoHIV is an established model for neuroAIDS, its effects on platelets are ill-defined. Our results indicate that EcoHIV behaves differently from HIV-1 and is cleared from circulation after 48 h post- infection. The EcoHIV course of infection resembles an HIV-1 infection under the effects of combined antiretroviral therapy (cART) since infected mice stayed immunocompetent and the virus was readily detected in the spleen. EcoHIV-infected mice failed to become thrombocytopenic and showed no signs of …


Loss Of Carnitine Palmitoyltransferase 1a Reduces Docosahexaenoic Acid-Containing Phospholipids And Drives Sexually Dimorphic Liver Disease In Mice, Mikala M. Zelows, Corissa Cady, Nikitha Dharanipragada, Anna E. Mead, Zachary A. Kipp, Evelyn A. Bates, Venkateshwari Varadharajan, Rakhee Banerjee, Se-Hyung Park, Nathan R. Shelman, Harrison A. Clarke, Tara R. Hawkinson, Terrymar Medina, Ramon C. Sun, Todd A. Lydic, Terry D. Hinds, Jr., J. Mark Brown, Samir Softic, Gregory A. Graf, Robert N. Helsley Dec 2023

Loss Of Carnitine Palmitoyltransferase 1a Reduces Docosahexaenoic Acid-Containing Phospholipids And Drives Sexually Dimorphic Liver Disease In Mice, Mikala M. Zelows, Corissa Cady, Nikitha Dharanipragada, Anna E. Mead, Zachary A. Kipp, Evelyn A. Bates, Venkateshwari Varadharajan, Rakhee Banerjee, Se-Hyung Park, Nathan R. Shelman, Harrison A. Clarke, Tara R. Hawkinson, Terrymar Medina, Ramon C. Sun, Todd A. Lydic, Terry D. Hinds, Jr., J. Mark Brown, Samir Softic, Gregory A. Graf, Robert N. Helsley

Saha Cardiovascular Research Center Faculty Publications

Background and aims: Genome and epigenome wide association studies identified variants in carnitine palmitoyltransferase 1a (CPT1a) that associate with lipid traits. The goal of this study was to determine the role of liver-specific CPT1a on hepatic lipid metabolism.

Approach and results: Male and female liver-specific knockout (LKO) and littermate controls were placed on a low-fat or high-fat diet (60% kcal fat) for 15 weeks. Mice were necropsied after a 16 h fast, and tissues were collected for lipidomics, matrix-assisted laser desorption ionization mass spectrometry imaging, kinome analysis, RNA-sequencing, and protein expression by immunoblotting. Female LKO mice had increased serum alanine …


A Novel Role Of Tgfbi In Macrophage Polarization And Macrophage-Induced Pancreatic Cancer Growth And Therapeutic Resistance, Jing Zhou, Nan Lyu, Qiongling Wanga, Ming Yang, Eric T. Kimchi, Kun Chenge, Trupti Joshi, Adama R. Tukulif, Kevin F. Staveley-O’Carroll, Guangfu Li Dec 2023

A Novel Role Of Tgfbi In Macrophage Polarization And Macrophage-Induced Pancreatic Cancer Growth And Therapeutic Resistance, Jing Zhou, Nan Lyu, Qiongling Wanga, Ming Yang, Eric T. Kimchi, Kun Chenge, Trupti Joshi, Adama R. Tukulif, Kevin F. Staveley-O’Carroll, Guangfu Li

Biomedical Sciences

Tumor-associated macrophages (TAMs), as a major and essential component of tumor microenvironment (TME), play a critical role in orchestrating pancreatic cancer (PaC) tumorigenesis from initiation to angiogenesis, growth, and systemic dissemination, as well as immunosuppression and resistance to chemotherapy and immunotherapy; however, the critical intrinsic factors responsible for TAMs reprograming and function remain to be identified. By performing single-cell RNA sequencing, transforming growth factor-beta-induced protein (TGFBI) was identified as TAM-producing factor in murine PaC tumors. TAMs express TGFBI in human PaC and TGFBI expression is positively related with human PaC growth. By inducing TGFBI loss-of-function in macrophage (MΦs) in vitro …


Sprouty4 Is Epigenetically Upregulated In Human Colorectal Cancer, Alexei J. Stuckel, Shuai Zeng, Zhen Lyu, Wei Zhang, Xu Zhang, Urszula Dougherty, Reba Mustafi, Tripti Khare, Qiong Zhang, Trupti Joshi, Marc Bissonnette, Sharad Khare Dec 2023

Sprouty4 Is Epigenetically Upregulated In Human Colorectal Cancer, Alexei J. Stuckel, Shuai Zeng, Zhen Lyu, Wei Zhang, Xu Zhang, Urszula Dougherty, Reba Mustafi, Tripti Khare, Qiong Zhang, Trupti Joshi, Marc Bissonnette, Sharad Khare

Biomedical Sciences

Sprouty4 (SPRY4) has been frequently reported as a tumor suppressor and is therefore downregulated in various cancers. For the first time, we report that SPRY4 is epigenetically upregulated in colorectal cancer (CRC). In this study, we explored DNA methylation and hydroxymethylation levels of SPRY4 in CRC cells and patient samples and correlated these findings with mRNA and protein expression levels. Three loci within the promoter region of SPRY4 were evaluated for 5mC levels in CRC using the combined bisulfite restriction analysis. In addition, hydroxymethylation levels within SPRY4 were measured in CRC patients. Lastly, DNA methylation and mRNA expression data were …


Oncolytic Tanapoxvirus Recombinants Expressing Mil-2, Flagellin-C, And Mccl2 Regress Human Cancer Xenografts In Immune Deficient Nude Mice And Immune Cell Reconstituted Balb/C Nude Mice., Michael Leonard Monaco Dec 2023

Oncolytic Tanapoxvirus Recombinants Expressing Mil-2, Flagellin-C, And Mccl2 Regress Human Cancer Xenografts In Immune Deficient Nude Mice And Immune Cell Reconstituted Balb/C Nude Mice., Michael Leonard Monaco

Dissertations

Tanapoxvirus (TPV) is a double stranded DNA virus from the genus Yatapoxvirus which has been engineered for the purpose of potentially treating a number of human cancers. Previous studies have shown TPV recombinants are capable of inducing tumor inhibition and even regression in athymic nude mice bearing human cancer xenografts of colorectal, melanoma and triple negative breast cancer (TNBC). However, TPV is host-restricted to humans and monkeys, including cancerous cells, which has provided a unique obstacle in its development as an oncolytic virus (OV). Namely, standard syngeneic mouse models cannot be used as test systems to evaluate TPV’s efficacy against …


Exploring The Potential Of Tesla Valve For Filtering And Sorting Microscale Active Swimmers: A Computational Study, Ariel Rogers, Zhiyao He, Yong Wang Dec 2023

Exploring The Potential Of Tesla Valve For Filtering And Sorting Microscale Active Swimmers: A Computational Study, Ariel Rogers, Zhiyao He, Yong Wang

Physics Faculty Publications and Presentations

It is well-known that a Tesla valve allows fluids to flow unidirectionally without moving parts; however, how Tesla valves interact with active matters and the potential applications of Tesla valves in biology remain largely unexplored. Here, we present a computational study on the potential use of Tesla valves for filtering and sorting microscale active swimmers such as bacteria. We investigated the behavior of microscale swimmers passing through the Tesla valve at different linear and angular velocities using numerical simulations and quantified the diodicity of the Tesla valve for active swimmers. Our results demonstrate that the Tesla valve can effectively filter …


A Muscle Exercise Research Revolution Powered By -Omics At Single Cell And Nucleus Resolution, Kevin A. Murach, Charlotte Peterson Dec 2023

A Muscle Exercise Research Revolution Powered By -Omics At Single Cell And Nucleus Resolution, Kevin A. Murach, Charlotte Peterson

Health, Human Performance and Recreation Faculty Publications and Presentations

In the last few years, single cell and single nucleus RNA-sequencing technologies have been leveraged in exercised adult skeletal muscle to reveal complex cell type-specific regulation of muscle plasticity. Expanding -omics technology at single cell resolution, complimented by advanced computational approaches, promise to rapidly accelerate our understanding of how exercise confers its numerous beneficial effects, paving the way for cell type-specific and targeted therapeutics to increase exercise responses during aging and in the face of chronic disease.


On The Origin And Evolution Of The Mosquito Male-Determining Factor Nix, James K. Biedler, Azedah Aryan, Yumin Qi, Aihua Wang, Ellen O. Martinson, Daniel A. Hartman, Fan Yang, Atashi Sharma, Katherine S. Morton, Mark Potters, Chujia Chen, Stephen L. Dobson, Gregory D. Ebel, Rebekah C. Kadling, Sally Paulson, Rui-De Xue, Michael R. Strand, Zhijian Tu Dec 2023

On The Origin And Evolution Of The Mosquito Male-Determining Factor Nix, James K. Biedler, Azedah Aryan, Yumin Qi, Aihua Wang, Ellen O. Martinson, Daniel A. Hartman, Fan Yang, Atashi Sharma, Katherine S. Morton, Mark Potters, Chujia Chen, Stephen L. Dobson, Gregory D. Ebel, Rebekah C. Kadling, Sally Paulson, Rui-De Xue, Michael R. Strand, Zhijian Tu

Entomology Faculty Publications

The mosquito family Culicidae is divided into 2 subfamilies named the Culicinae and Anophelinae. Nix, the dominant male-determining factor, has only been found in the culicines Aedes aegypti and Aedes albopictus, 2 important arboviral vectors that belong to the subgenus Stegomyia. Here we performed sex-specific whole-genome sequencing and RNAseq of divergent mosquito species and explored additional male-inclusive datasets to investigate the distribution of Nix. Except for the Culex genus, Nix homologs were found in all species surveyed from the Culicinae subfamily, including 12 additional species from 3 highly divergent tribes comprising 4 genera, suggesting Nix originated at least 133 to …


How Sewage Pollution Affects Distribution And Life History Traits Of The Southern House Mosquito, Culex Quinquefasciatus, Catherine Dean Bermond Dec 2023

How Sewage Pollution Affects Distribution And Life History Traits Of The Southern House Mosquito, Culex Quinquefasciatus, Catherine Dean Bermond

Master's Theses

Eutrophication from wastewater can cause fluxes of nutrients like and carbon and nitrogen in urban waters. One source of nitrogen in freshwater is from untreated sewage discharging from septic tanks. This causes a variety of environmental issues including harmful algal blooms, harming natural predators, and increased production of mosquitoes. Culex quinquefasciatus is the vector of West Nile Virus in St. Tammany Parish, Louisiana. St. Tammany Parish has over 600 miles of septic ditches, which receive effluent directly from aerated treatment units. Culex quinquefasciatus oviposit in polluted waters, and larvae perform well in water with high levels of decomposing organic matter. …


Evaluating Habitat Use By Nekton In Widgeon Grass (Ruppia Maritima), Shoal Grass (Halodule Wrightii), And Unvegetated Bottom Habitats In The Grand Bay National Estuarine Research Reserve, Jessica Woodall Dec 2023

Evaluating Habitat Use By Nekton In Widgeon Grass (Ruppia Maritima), Shoal Grass (Halodule Wrightii), And Unvegetated Bottom Habitats In The Grand Bay National Estuarine Research Reserve, Jessica Woodall

Master's Theses

Seagrass beds support high biodiversity and animal abundance, serve as feeding grounds for a variety of animals, offer shelter from predation, and act as a nursery habitat for juveniles. The species composition of seagrass beds can impact their use as habitat by animals. Two common species of seagrass in the Gulf of Mexico are Ruppia maritima (widgeon grass) and Halodule wrightii (shoal grass). The shallow coastal waters of the Grand Bay National Estuarine Research Reserve (NERR) support both species, but the use of each seagrass as habitat by nekton is poorly understood, which can limit management decision-making. Nekton communities were …