Life Sciences Commons^™

Biological Condition Of Money Pont Benthic Communities, Southern Branch Of The Elizabeth River (2010, 2013, 2016), Daniel M. Dauer

Elizabeth River Reports

Executive Summary

This report summarizes the ecological condition of the subtidal macrobenthic communities off Money Point in the Southern Branch of the Elizabeth River based upon quantitative sampling in summer 2016. The designated Money Point study area was part of a sediment contaminant remediation effort. The primary objectives were to: (1) characterize the biological health of the benthos of Money Point comparing pre-remediation condition (2010) to post-remediation condition in 2013 and again in 2016, and (2) assess the effectiveness of the sediment contamination remediation efforts with respect to the ecological condition of the Money Point benthos.

Prior to sediment contaminant …

Salinity Tolerance Of Gambusia Affinis, Sarah Rubelowsky

Honors Theses

Developmental plasticity refers to changes during development as a result of environmental contributions. Salinity is a varying environmental condition in freshwater and estuarine habitats that can mediate developmental plasticity in Gambusia affinis, which can increase their tolerances as an invasive species. For my study, estuarine and freshwater populations of Gambusia affinis were sampled in March of 2017 using dip nets. Both populations were then brought back to the laboratory where pregnant females were acclimated to three different salinities (0‰, 15‰, 25‰) until they gave birth. I predicted that the estuarine population of Gambusia affinis would have a greater salinity tolerance …

Morphological, Genetic, And Environmental Characterization Of An Unusual Population Of Isoetes (Isoetaceae, Lycopodiophyta), Shannon L. Walker

Honors Theses

A large and unusual population of Isoetes (Isoetaceae, Lycopodiophyta) in the DeSoto National Forest, Wayne County, Mississippi, was studied to determine if the individuals there represent a new species or if they represent part of the variation of the one primary species of the longleaf pine belt of Mississippi, Isoetes louisianensis, which it most closely resembles. The unusual population and specimens of known Isoetes louisianensis were examined comparatively based on morphology, megaspore ornamentation, examination of habitat characteristics, and phylogenetic analysis of DNA sequence data from the nuclear internal transcribed spacer 1 and 2 (ITS) and the 5.8S ribosomal gene. No …

Genome Sequences Of Mycobacteriophages Amgine, Amohnition, Bella96, Cain, Darthp, Hammy, Krueger, Lasthope, Peanam, Phelpsodu, Phrank, Sirphilip, Slimphazie, And Unicorn, Kirk R. Anders, Nazir Barekzi, Aaron A. Best, Gregory D. Frederick, Dmitri V. Mavrodi, Edwin Vasquez, Sea-Phages, Nana Yaa A. Amoh, Frederick N. Baliraine, William J. Buchser, Thomas P. Cast, Carmen E. Chamberlain, Hui-Min Chung, William A. D'Angelo, Christian T. Farris, Mariceli Fernandez-Martinez, Haley D. Fischman, Mark H. Forsyth, Anna G. Fortier, Kara F. Gallo, Greta J. Held, Miguel A. Lomas, Natalia Y. Maldonado-Vasquez, Claudia H. Moonsammy, Peace Namboote, Sudip Paudel, Sarah-Elizabeth Polley, Gabriella Reyes, Michael R. Rubin, Margaret S. Saha, Joseph Stukey, Tristan D. Tobias, Rebecca A. Garlena, Ty H. Stoner, Steven G. Cresawn, Deborah Jacobs-Sera, Welkin H. Pope, Daniel A. Russell, Graham F. Hatfull

Faculty Publications

We report the genome sequences of 14 cluster K mycobacteriophages isolated using Mycobacterium smegmatis mc²155 as host. Four are closely related to subcluster K1 phages, and 10 are members of subcluster K6. The phage genomes span considerable sequence diversity, including multiple types of integrases and integration sites.

A Deafness Mechanism Of Digenic Cx26 (Gjb2) And Cx30 (Gjb6) Mutations: Reduction Of Endocochlear Potential By Impairment Of Heterogeneous Gap Junctional Function In The Cochlear Lateral Wall, Ling Mei, Jin Chen, Liang Zong, Yan Zhu, Chun Liang, Raleigh O. Jones, Hong-Bo Zhao

Otolaryngology--Head & Neck Surgery Faculty Publications

Digenic Connexin26 (Cx26, GJB2) and Cx30 (GJB6) heterozygous mutations are the second most frequent cause of recessive deafness in humans. However, the underlying deafness mechanism remains unclear. In this study, we created different double Cx26 and Cx30 heterozygous (Cx26^+/−/Cx30^+/−) mouse models to investigate the underlying pathological changes and deafness mechanism. We found that double Cx26^+/−/Cx30^+/− heterozygous mice had hearing loss. Endocochlear potential (EP), which is a driving force for hair cells producing auditory receptor current, was reduced. However, unlike Cx26 homozygous knockout (Cx26^−/−) mice, the cochlea in Cx26 …

Analytical Modeling Of A Communication Channel Based On Subthreshold Stimulation Of Neurobiological Networks, Alireza Khodaei

School of Computing: Dissertations, Theses, and Student Research

The emergence of wearable and implantable machines manufactured artificially or synthesized biologically opens up a new horizon for patient-centered health services such as medical treatment, health monitoring, and rehabilitation with minimized costs and maximized popularity when provided remotely via the Internet. In particular, a swarm of machines at the scale of a single cell down to the nanoscale can be deployed in the body by the non-invasive or minimally invasive operation (e.g., swallowing and injection respectively) to perform various tasks. However, an individual machine is only able to perform basic tasks so it needs to exchange data with the others …

Santa Clara County Senior Nutrition Program Evaluation Report, Tae Hwan Ihm

Master's Projects

Santa Clara County conducts a Senior Nutrition Program (SNP) in conjunction with the City of San Jose, the Outreach Paratransit Program and local community-based organizations (CBOs) like Sourcewise (Lam, 2015). These programs combined represent a significant investment of public funds, with more than 45% of the SNP program’s funding coming from the county General Fund (Lam, 2015). Such a large financial commitment needs to demonstrate some benefit to the participants, and some positive outcomes from the current program design. This study evaluated the SNP’s effectiveness in achieving its primary goals. Primary goals of the SNP are to promote senior citizens’ …

Mechanisms Of Plasmodium-Enhanced Attraction Of Mosquito Vectors, Annette O. Busula, Niels O. Verhulst, Teun Bousema, Willem Takken, Jetske G. De Boer

All Peer-Reviewed Publications

Evidence is accumulating that Plasmodium-infected vertebrates are more attractive to mosquitoes than noninfected hosts, particularly when high levels of gametocytes are present. Changes in host odour have been suggested as a likely target for parasite manipulation because olfactory cues are crucial to mosquitoes in search of a bloodmeal host. This review discusses two routes that may lead to such changes: (i) direct emission of volatile products from malaria parasites, and (ii) changes in skin microbial composition that could lead to changes in the vertebrate odour profile. Here we synthesize what is known and suggest how further research can increase our …

Welwitschianalol A And B, Two Cyclohexene Derivatives And Other Insecticidal Constituents Of Caesalpinia Welwitschiana (Oliv.) Brenan, Flaure Rosette Ehawa Essoung, Brice Mittérant Mba'ning, Wilber Lwande, Silvère Augustin Ngouela, Etienne Tsamo, Sumesh Chander Chhabra, Ahmed Hassanali, Russell John Cox

All Peer-Reviewed Publications

Crude methanol extract of the leaves of Caesalpinia welwitschiana was found to possess moderate larvicidal activity against second-instar larvae of Tuta absoluta Meyrick. Bioassay-guided fractionation of this extract led to the isolation of two oxygenated cyclohexene derivatives, welwitschianalol A (1, (1α,2β)-2-acetoxy-1-hydroxy-1- benzoyloxymethylcyclohex-5-ene) and B (2, (1α,2β)-2- benzoyloxy −1-cinnamoyloxymethyl-1-hydroxycyclohex-5-ene), together with eight known compounds, comprising bonducellin, dipteryxic acid, 3′-hydroxygenkwarin, afzelin, quercitrin, myricitrin, methyl gallate and gallic acid. The structures of the unknown compounds as well as those of the known ones were elucidated based on their MS, 1D and 2D NMR spectra, and in the case of known compounds, by comparison …

Accounting For Soil Inorganic Carbon In The Ecosystem Services Framework For United Nations Sustainable Development Goals, Garth Raymond Groshans

All Theses

Soil inorganic carbon (SIC) is currently not included in the list of key soil properties related to ecosystem services (e.g., provisioning, regulating, cultural, and supporting services). Soil inorganic carbon is a dynamic key soil property used in soil classification, taxonomy and fertility, therefore its inclusion in the framework of ecosystem services is important. With soils rapidly changing due to human use and climate change, the soil ecosystem services framework should not include only soil organic carbon (SOC), but SIC as well since it is of global importance to soil fertility and the long-term carbon cycle, especially in semiarid and arid …

National Smokejumper Assocation Trail Maintenance Annual Report For 2017, National Smokejumper Association Trails Committee

Trails Reports

The Trails Maintenance Program Annual Reports compiled by the National Smokejumper Association Trails Committee summarizes the activities of the Trails Program. The members of the program also go by the title Trails Restoration and Maintenance Project Specialists (TRAMPS). The contents of the 2011 report include: Volunteers of the year; Fred Cooper; Steve Carlson; Admin information; BWCAW; Monture GS; Wild Flower Trail; Big Pryor Mtn. Trail; Reese Cabin Firewood; Horse Camp Fishlake; Dixie; Rocky Mtn. NP; Sawtooth; Stanley Basin; Rio Hondo Cabin; Lewis & Clark; Mann Gulch; Lassen NP; Rio Puerco Trail; Antone Cabin; West Fork Cabin; Yellow Jacket Lake; Santa …

Lake Sturgeon (Acipenser Fulvescens) Trophic Position And Movement Patterns In The Lower Niagara River, Ny, Eric Bruestle

Great Lakes Center Masters Theses

Lake sturgeon Acipenser fulvescens were once widely distributed throughout the Laurentian Great Lakes. However, widespread overharvest and habitat degradation has diminished their numbers. The lower Niagara River, NY contains one of the few remaining recovering populations of lake sturgeon in New York State. The goal of this study was to characterize the trophic position of lake sturgeon in the context of an invasive species dominated food web and to describe their movement patterns and residency within the lower Niagara River. Stomach content analysis and stable nitrogen (δ¹⁵N) and carbon (δ¹³C) isotope analysis of tissue was used …

Flushing Responses Of Golden Eagles (Aquila Chrysaetos) In Response To Recreation, Robert J. Spaul, Julie A. Heath

Biology Faculty Publications and Presentations

Disturbance because of human activity, including recreation on wildlands, can affect bird behavior which in turn can reduce breeding success, an important consideration for species of management concern. We observed Golden Eagles (Aquila chysaetos) during the breeding season to determine whether the probability of flushing was affected by the type of recreationist, distance to encounter, eagle nest attendance, or date. We monitored eagles in 23 nesting territories from distant (600-1,200 m) observation points and recorded recreation activity within 1,200 m of eagles in the Owyhee Front of southwestern Idaho. In most (86%, n = 270) encounters, eagles did …

Commentary: Research Recommendations For Understanding The Decline Of American Kestrels (Falco Sparverius) Across Much Of North America, Christopher J.W. Mcclure, Sarah E. Schulwitz, Richard Van Buskirk, Benjamin P. Pauli, Julie A. Heath

Biology Faculty Publications and Presentations

Across much of North America, populations of American Kestrels (Falco sparverius) have been in decline for decades (Farmer et al. 2008, Farmer and Smith 2009, Smallwood et al. 2009a, Paprocki et al. 2014, Sauer et al. 2014). Hypothesized causes of kestrel declines include predation by Cooper's Hawks (Accipiter cooperii; Farmer et al. 2008), pathogens (e.g., Nemeth et al. 2006), habitat loss (Sullivan and Wood 2005, Farmer et al. 2008, Bolgiano et al. 2015), pesticides (Smallwood et al. 2009a, Rattner et al. 2015), and climate change (Steenhof and Peterson 2009b), yet no hypothesized factor has been supported …

Preschoolers And Pandas Making Friends: A Journey About Healing From Brain Injury, Barbara Anne Doucette

Museum Studies Projects

Preschoolers that have obtained Non-Accidental Injury (NAI) from familial child abuse are in need of having a unique place for neurorehabilitation in correlation with traditional therapies. My thesis project suggests adding an exhibit annex to an existing giant panda exhibit that will give preschoolers an opportunity to help develop new neuropathways when exposed to mediation and creative activities. Meditation and creative activities are being examined by neuroscientists as an aid in neuroplasticity after brain injury. This thesis reviews the neurotypical preschooler’s milestones and the playful means by which they are achieved. Conjoining the contemporary museums’ and zoological gardens’ outreach to …

Phosphorus Retention In West Michigan Two-Stage Agricultural Ditches, Emily Kindervater

Masters Theses

Input of excess nutrients into a water body can negatively impact ecological structure and function, as well as the economic vitality of surrounding communities, by contributing to eutrophication. For example, phosphorus (P) and sediment inputs from agricultural drainage have facilitated the development of hypereutrophic conditions in Lake Macatawa, a drowned river mouth lake located in Holland, Michigan. Two-stage ditches, an agricultural best management practice (BMP), are used in some areas of the Midwest to reduce N export downstream via denitrification. This BMP simulates a mini-floodplain by replacing a traditional, trapezoidal ditch with a channel that has excavated benches on each …

Brook Trout Behavioral Thermoregulation And Habitat Selection In A Small Michigan Coldwater Stream: Implications For Successful Management, Justin Emory Wegner

Masters Theses

Global warming and conversion of forests for urbanization, agriculture and mineral extraction are increasing water temperatures throughout Brook Trout’s range causing population declines; particularly in populations persisting in marginal habitats and in the southern limits of their distribution. The Brook Trout is an ectotherm that can cope with elevated water temperature by moving to coldwater refuge such as groundwater seeps, coldwater tributary confluences, and headwaters. Availability of coldwater refuge is vital for the survival of Brook Trout populations threatened by increasing water temperatures. I used radio telemetry to study the movement, habitat use, and behavioral thermoregulation of Brook Trout living …

General Survey Of Fructus Psoraleae From The Different Origins And Chemical Identification Of The Roasted From Raw Fructus Psoraleae, Junjun Yang, Jing Yang, Jie Du, Benjamin Feng, Xin Chai, Mingming Xiao, Yuefei Wang, Xiumei Gao

Peer Reviewed Articles

Fructus Psoraleae, a traditional Chinese medicine, is widely used for preventing and treating various diseases such as vitiligo, osteoporosis and psoriasis. Coumarin, such as psoralenoside, isopsoralenoside, psoralen and isopsoralen, are important compounds in Fructus Psoraleae. In our study, ultra performance liquid chromatography coupled with diode array detector was employed for an excellent method validation for simultaneous quantification of psoralenoside, isopsoralenoside, psoralen and isopsoralen, which was further applied in performing general survey of Fructus Psoraleae from the different origins and chemical identification of the roasted from raw Fructus Psoraleae in the light of illuminating the transformed rule of psoralenoside and isopsoralenoside. …

Surveying The Circular Rep Encoding Single Stranded (Cress) Dna Viral Consortium Found In Invasive Quagga Mussels (Dreissena Rostriformis Bugensis) And Sediments Of The Central Lake Michigan Benthos, Nicholas Gezon

Masters Theses

Globalization and resulting increases in international trade has allowed for the movement of species between almost all ecosystems on Earth. The Great Lakes in particular has seen a number of high impact invasive species that have moved in following the opening of the lakes to intercontinental shipping trade. This research specifically focuses on the benthic community of Lake Michigan which has seen significant community shifts including declines of the amphipods Diporeia spp. and the expansion of invasive quagga mussels (Dreissena rostriformis bugensis). Little is known about the viral community in the benthos of Lake Michigan and how …

The Spatial Ecology And Microhabitat Selection Of The Pygmy Rattlesnake (Sistrurus Miliarius) In Southwestern Missouri, Dylan Wallace Maag

Graduate Theses/Dissertations

Despite a wide distribution throughout the southeastern United States, pygmy rattlesnakes (Sistrurus miliarius) have received less research attention than many other rattlesnake species. I captured a total of 33 S. miliarius at the Drury-Mincy Conservation Area (DMCA) and retained 14 large individuals (mostly gravid females) for a radio telemetry study. Snakes were primarily captured during evening road driving surveys and were encountered rarely with any other sampling technique. Sistrurus miliarius are widespread at DMCA where they were encountered in forest, savanna, and glade habitats. Snakes selected microhabitats with more vegetative cover and tree canopy closure than random sites …

The Effect Of Weight Carried And Time Ridden On Back Pain In Horses Ridden During Horse Shows As Determined By Pressure Algometry, Meghan Louise Mothershead

Graduate Theses/Dissertations

The objective of this study was to determine the effects of weight carried (rider + tack) on back pain in horses used in riding classes and a series of intercollegiate horse shows. Sixteen school horses (431-649 kg) were ridden consistently over a semester. Cross sectional images were used to measure width and put in a mapping program to figure total area. Back pain scores were determined using a pressure algometer on predetermined points over the horses back. Pain scores were recorded over five periods, including 28 d into the semester (P1) and during the show period on three consecutive Fridays …

Shallow Aggregate Ebb-And-Flow System For Greenhouse Lettuce Production, Joseph Chidiac

Graduate Theses and Dissertations

A novel soilless technique for the production of lettuce was developed and evaluated for its viability for ornamental greenhouse growers adapting their ebb-and-flood irrigation benching systems to diversify into growing lettuce, without having to purchase the conventional nutrient film technique (NFT) or deep flow technique (DFT) hydroponic systems. The experimental design was a three by four factorial, with three treatments for aggregate depth (19, 38, and 57 mm) and four treatments for irrigation frequency (once every 1, 2, 4, or 8 hours), for a total of twelve treatment combinations. Each treatment was replicated three times in a randomized complete block …

Transcriptome-Based Gene Networks For Systems-Level Analysis Of Plant Gene Functions, Chirag Gupta

Graduate Theses and Dissertations

Present day genomic technologies are evolving at an unprecedented rate, allowing interrogation of

cellular activities with increasing breadth and depth. However, we know very little about how the

genome functions and what the identified genes do. The lack of functional annotations of genes

greatly limits the post-analytical interpretation of new high throughput genomic datasets. For plant

biologists, the problem is much severe. Less than 50% of all the identified genes in the model plant

Arabidopsis thaliana, and only about 20% of all genes in the crop model Oryza sativa have some

aspects of their functions assigned. Therefore, there is an …

In The Field The Women Saved The Crop: The Women’S Land Army Of World War Ii, Denna M. Clymer

Graduate Theses and Dissertations

The Women’s Land Army brought together rural and urban sectors of the United States in a climate of national and regional crisis. By the time the country was cast into war, the agricultural sector was already caught in a downward economic spiral that drove away laborers. With demand falling, and farms propped up only by experiments in subsidy and parity, when military and industrial jobs emerged in urban areas, farm laborers became scarce. At the same time the war created jobs for men outside of the agricultural sector, farm prices recovered and demand soared, forcing farmers to look to women …

Fall Risk In Sedentary, Recreationally Active, And Masters Athlete Older Adults, Felicia Elizabeth Squires

Graduate Theses and Dissertations

The purpose of this study was to examine whether different habitual activity levels may affect balance ability in older adults. Balance must be studied as poor balance increases likelihood of falls. This study examined activity levels in older adults and effect on balance ability. Fifty-nine older adults aged 66.5 ± 9.5 participated. Three groups were separated by physical activity level. First group had 13 masters athletes, older adults performing least 150 minutes of aerobic activity a week and competing in nationally sanctioned event within the last six months. Twenty-seven older adults comprised recreationally active group performing at least 150 minutes …

Identifying Genetic Factors Influencing Sperm Mobility Phenotype In Chicken Using Genome Wide Association Studies, Primordial Germ Cell Transplantation, And Rnaseq., Sohita Ojha

Graduate Theses and Dissertations

Sperm mobility is a major determinant of male fertility in chicken. In spite of low heritability of reproductive traits, sperm mobility has high heritability index which suggests presence of quantitative trait loci (QTLs) governing the trait. Our research focused on three objectives: i) to identify the QTLs affecting low mobility phenotype in chicken, ii) to understand the impact of Sertoli-cells and germ cells interactions in influencing the mobility phenotype and iii) to identify the genes and gene networks differentially expressed in male and female PGCs. To detect the QTLs, genome wide association studies (GWAS) was conducted which revealed the presence …

A Study Of The Prevalence Of Gastrointestinal Nematodes In Goats Obtained From Northwest Arkansas, Christine Rose Weingartz

Graduate Theses and Dissertations

Parasitic helminths have always been an issue in small ruminant production; pathogens that pose great negative impact on goat health and productivity. Insufficient work has been done to document the prevalence of parasitic helminths in the United States, especially in the south and southeast, where the largest goat populations are found.

The aim of this study was to survey the prevalence of infections by gastrointestinal nematodes in goats in Northwestern Arkansas. Gastrointestinal tracts were examined from 41 goats of various locations around Northwest Arkansas. Worm species were identified and population burdens were determined. Coprology was correlated with the actual worm …

Characterization Of Multiple-Herbicide-Resistant Echinochloa Colona From Arkansas, Christopher Edward Rouse

Graduate Theses and Dissertations

Echinochloa species are highly adaptive weeds that have the potential to impact crops in a variety of environments. This has positioned them as the most problematic weeds in a number of USA cropping systems with some species having the distinction of the 'worst herbicide-resistant weeds' in the world. Recent evidence has positioned Echinochloa colona (junglerice) as the most dominant in Arkansas and throughout the Mid-South, USA, especially in rice (Oryza sativa L.) and soybean (Glycine max L.) production fields. A history of extensive herbicide-use for management and a lack of integrated or diverse approaches to management have led to rampant …

Experimental Approaches To Understand And Control Salmonella Infection In Poultry, Yichao Yang

Graduate Theses and Dissertations

Salmonella is a major foodborne pathogen around the world and chickens are the major reservoir to transmit Salmonella into the human food chain. For decreasing the infection of Salmonella, we developed six attenuated live vaccines based on Salmonella Enteritidis (SE) and Typhimurium (ST) for testing the cross-serovar and cross-serogroup protection from the challenge of Salmonella Heidelberg and Campylobacter jejuni. One of the constructed vaccine strain showed ability to protect against challenge from Salmonella Heidelberg. Even though some preventive approaches are able to decrease Salmonella colonization in the gastrointestinal tract of chickens or other farm animals, Salmonella transmission mechanisms remain unclear. …

Evaluation Of Very-Long-Chain Fatty Acid-Inhibiting Herbicides In Arkansas Rice Production, John Ansley Godwin

Graduate Theses and Dissertations

Because of the evolution of herbicide-resistant weeds, such as barnyardgrass and red rice in rice, there is a need for alternative herbicide sites of action. Very-long-chain fatty acid (VLCFA)-inhibiting herbicides are not labeled for use in U.S. rice production; however, this site of action (SOA) has been used with success in Asian rice. The VLCFA-inhibiting herbicides pethoxamid, pyroxasulfone, acetochlor, and S-metolachlor were evaluated for rice tolerance and control of commonly problematic weeds in Arkansas rice at various rates and application timings. Pyroxasulfone and S-metolachlor were deemed unfit for use in rice production because of negative effects on rice visual injury, …