Life Sciences Commons^™

Cellubrevin/Vesicle-Associated Membrane Protein-3–Mediated Endocytosis And Trafficking Regulate Platelet Functions, Meenakshi Banerjee, Smita Joshi, Jinchao Zhang, Carole L. Moncman, Shilpi Yadav, Beth A. Bouchard, Brian Storrie, Sidney W. Whiteheart

Molecular and Cellular Biochemistry Faculty Publications

Endocytosis is key to fibrinogen (Fg) uptake, trafficking of integrins (αIIbβ₃, α_vβ₃), and purinergic receptors (P2Y₁, P2Y₁₂), and thus normal platelet function. However, the molecular machinery required and possible trafficking routes are still ill-defined. To further identify elements of the platelet endocytic machinery, we examined the role of a vesicle-residing, soluble N-ethylmaleimide factor attachment protein receptor (v-SNARE) called cellubrevin/vesicle-associated membrane protein-3 (VAMP-3) in platelet function. Although not required for normal platelet exocytosis or hemostasis, VAMP-3^−/− mice had less platelet-associated Fg, indicating a defect in Fg uptake/storage. Other granule …

Structure And Properties Of Acetylated Corn Starch Prepared Under Ultra High Pressure (Uhp), Pu Huayin, Ma Yun, Huang Meng, Xu Yao, Fan Fan, Liu Shuhan, Huang Junrong

Food and Machinery

The acetylated starches from corn were prepared under ultra high pressure (UHP) by acetic anhydride as reaction reagent, and NaCl as the reaction medium. The structure and properties of acetylated starches were analyzed by using light microscope, X-ray diffractometer and rapid viscosity analyzer. The results indicated that the granular acetylated starch showed a similar crystallite structure as that of native starch. In addition, the acetylated starches were gelatinized when treated at 600 MPa and the structure of starch granules was gradually destroyed, whereas the crystal structure was transformed from the A-type crystallite to the V-type crystallite. However the gelatinization negatively …

The Analysis And Evaluation Of Nutrients Of Sansui’S Characteristic Sheldrake Duck Eggs, Shen Changxuan, Wang Xiujun, Huang Shan

Food and Machinery

The eggs of Sansui’s characteristic sheldrake duck were researched to enrich its foundation and deepen the objective evaluation. Moisture content, crude protein content, amino acid composition and its content, crude fat content, composition and content of fatty acids, cholesterol content and the content of calcium, zinc, magnesium, iron, copper, manganese, lead and selenium of 3 kinds of egg samples in Guizhou were determined by the methods of China national standard, chromatography of gases, Inductively coupled plasma atomic emission spectrometry and spectrofluorimetry. Amino acid and fatty acids were scientifically evaluated by the method WHO/FAO Amino Acid Scoring Model, the chemical score …

Study On Impact Characteristics Of Rubber Seeds, Li Zihao, Wang Tao

Food and Machinery

It is an important method to break rubber seeds with the principle of collision. In order to study the breaking mechanics of rubber seeds in the collision process, the instantaneous impact force of rubber seeds was measured by RFP data acquisition system. In this experiment, single factor method was adopted to select the height, angle and initial velocity factors to analyze the influence of various factors on the impact force of rubber seeds. The experimental results showed that (the perspective of the range is 0° to 90°) : When height increased from 0.5 m to 2.0 m, the collision force …

Effects Of 60Co Γ Ray Irradiation On Sterilization And Quality Of Sauced Chicken Claw, Peng Ling, Xu Yuanfang, Zhang Qiling, Zhou Yiji, Mao Qingxiu, Li Wenge

Food and Machinery

The effects of the ⁶⁰Co γ ray irradiation preservation on the sauced chicken claw were investigated by using different irradiation doses. The relationship was analyzed between the irradiation dose and the total bacterial count, nutrients, the composition of amino acids and fatty acids, AC, POV, TVB-N, nitrite as well as chroma. The results showed that the ⁶⁰Co γ ray irradiation was effective on sterilization, and while the sample was irradiated at the dose of 4.09 kGy, with the total bacterial count reduced to no more than 4.09 kGy. However, no significant effect of the ⁶⁰Co γ ray …

Immune Checkpoint Inhibitors In Neuroendocrine Tumors: A Single Institution Experience With Review Of Literature, Aman Chauhan, Millicent Horn, Gray Magee, Kurt Hodges, B. Mark Evers, Susanne Arnold, Lowell B. Anthony

Markey Cancer Center Faculty Publications

This unique case series and review of literature suggests that immune checkpoint inhibitors may have clinical activity in neuroendocrine tumors.

Objective: Summarize advances of immuno-oncology in neuroendocrine tumors with the help of a case series.

Design: Case series and review of literature.

Intervention or Exposure: The patients were treated with immune checkpoint inhibitors (pembrolizumab or nivolumab).

Main Outcome(s) and Measures(s): Life expectancy, quality of life, disease progression.

Results: Maximum durable response of 16 months in one of the patients so far. All patients showed improvement in quality of life before disease progression. Two out of four are still on therapy. …

Developmental Biology, The Stem Cell Of Biological Disciplines, Scott F. Gilbert

Biology Faculty Works

Developmental biology (including embryology) is proposed as "the stem cell of biological disciplines.” Genetics, cell biology, oncology, immunology, evolutionary mechanisms, neurobiology, and systems biology each has its ancestry in developmental biology. Moreover, developmental biology continues to roll on, budding off more disciplines, while retaining its own identity. While its descendant disciplines differentiate into sciences with a restricted set of paradigms, examples, and techniques, developmental biology remains vigorous, pluripotent, and relatively undifferentiated. In many disciplines, especially in evolutionary biology and oncology, the developmental perspective is being reasserted as an important research program.

Study On Relationship Of Quaity Between Wheat Quality And Fresh Noodles, Deng Hang, Zhou Wenhua, Li Lihua

Food and Machinery

In this study, five different varieties of Chinese wheats: “Jimai 22”, “Yannong 19”, “Yannong 5158”, “Aikang 58”, “Lunxuan 988” from the main producing area in China: Shanxi, Anhui and Henan were selected. The main physical and chemical characters, such as the content of protein, ash, starch, et al, as well as the farinograph property of flour milled by those five different varieties of wheats, were described, and the sensory evaluation and textural properties (TPA) of fresh noodle made from those flour were determined. The correlation between wheat quality and farinograph property and quality of fresh noodles were all analyzed. The …

Study On Micronization Of Spi Emulsions By Ultrasonication And Microfluidization Techniques, Feng Lili, Wu Xue, Huang Zhigang, Yu Jian

Food and Machinery

The purpose of the study was to produce soy protein isolate(SPI) micro emulsion droplet by ultrasonication and microfluidization Techniques. The changes of SPI emulsion temperature, average droplet diameter and size distribution were observed. The distribution curve of SPI droplet processed by ultrasonication has two peaks and that of SPI droplet processed by microfluidization has single peak. The results indicate that the SPI emulsion processed by high pressure is more homogeneous. The capability of reducing size by ultrasonic process (1 200 W) is very limited, and the SPI emulsion tends to agglomerate after a long time of ultrasonic process. Microfluidization (microseconds, …

Study On Correlation Between Sound Of Breaking And Crispness Of Potato Chips, Zhang Zihan, Feng Tao, Liang Yi, Feng Lili, Wang Jing

Food and Machinery

The hardness and crispness of potato chips were tested by texture analyzer in the present study. Moreover, the fractured acoustic signals were collected to analyze the relationship between the crispness of potato chips and the acoustic characteristics, and the acoustic rating of physical properties were evaluated. The relationship between the hardness crispness and flexibility of three different potato chips and their acoustic characteristics were investigated. The results showed that the more crispness of potato chips had, their hardness and toughness were lower, and the sound of breaking was louder. However, no significant linear correlation were found between the characteristics.

Quality Indifferent Degrees Of Softness Of Pickles And Itsprincipal Components Analysis Of Volatiles, Huang Shenglan, Du Muyin, Zhou Xianrong, Tang Yanyan, Li Yuzhu, Long Mou, Yu Lihong

Food and Machinery

Taking softened radish pickles as research object, the present study explored the differences in physicochemical property and flavor substance of normal pickles and pickles with different softness, determined the flavor and analyzed the main ingredients of pickles with eadspace solid-phase microextraction-gas chromate graphy-mass spectrometry, HS-SPME-GC-MS. The experimental results showed that the acidity and salinity of softened pickles were higher than those of normal pickles. The texture index, sensory evaluation of normal pickles were all better than softened pickles, and the 4 kinds of pickles were all yellowish green. 41, 32, 37 and 30 volatile ingredients were detected in normal pickles …

Analysis And Comparison On Solid Ispersion Performance Of Dandelion Chlorogenic Acid, Li Yan, Wang Jie, Cao Keke, Wang Zhanggui, Wang Di

Food and Machinery

To use the solid dispersion method that could improve the dissolution rate of chlorogenic acid.With the factor experiment design, chlorogenic acid solid was mixed with PVPK30 and PEG4000 respectively, with the feed auxiliary ratio of 12, 14, 16, 18. The finished product of solid dispersion prepared by four types of mixture ratio were compared in the vitro solubility, cumulative dissolution and stability, and the optimal mixture ratio was analyzed by SEM. The experiment showed that chlorogenic acid solid dispersion could be prepared by mixture ratio of 14, with PVPK30 as carrier. Compared with crude drugs, in vitro solubility and dissolution …

Analyses Of The Volume Of The Polybasic Acids And Higher Fatty Acids And Their Effect On Tobacco Quality, Xue Yun, Long Zhangde, Yan Jun, Liu Hong, Li Xinqin, Hu Zhizhong, Pan Yongcheng, Lin Shunshun, Zhang Xiaoming

Food and Machinery

The relationship of polybasic acids and higher fatty acid content with sensory quality of tobacco raw materials from the different tobacco growing area in Hunan province was investigated, using non derivatization gas chromatographic method. Seven tobacco samples were collected from three districts/counties, and the content of polybasic acids and higher fatty acids of them were detected. The results showed that the highest content of seven kinds of tobacco among the polybasic acids was propionic acid, followed by 2-Methylbutyric acid and 3-methylvaleric acid. Among the higher fatty acids, the highest content of seven kinds of tobacco was palmitic acid, followed by …

Antibacterial Activity Of Fractions And Chemical Constituents Analysis Of Dichloromethane Fraction From Bletilla Striata, Wu Yongxiang, Cheng Manhuai, Jiang Haitao, Hou Shuzeng, Wan Zhibing

Food and Machinery

Bletilla striata extracts were prepared by methanol circumfluence extraction. Five different polarity fractions were obtained by extraction in turn with n-hexane, dichloromethane, ethyl acetate, n-butanol and water. The inhibiting effects on E. coli, S.aureus, B.subtilis and P.aeruginos were measured by using filter paper disc diffusion method and minimum inhibitory concentration (MIC) method. The chemical constituents of the active fraction were analyzed by gas chromatography-mass spectrometry (GC-MS). The results showed that the methanol extract, n-hexane and dichloromethane fractions exhibited significant inhibition against four common bacterial strains. Dichloromethane fraction presented the best antibacterial effect than other fractions (P＜0.05). Dichloromethane fraction exerted potent …

Extrusion Analysis Of Tianzi-Shaped Tray Of Food Based On Polyflow, Jiang Weixin, Huang Zhigang, Zhao Yulian, Gu Jin, Wang Xiaofeng, Zhang Kai

Food and Machinery

Using Solidworks 3D modeling software to establish the model of Tian-Shaped plastics profile extrusion die. One-quarter part of the Die was divided into ANSYS grid. The melt of poly lactic acid was used as fluid at 190 ℃ in Polyflow simulation analysis. The flow field simulation results of the normal die and the design of the inverse extrusion die were obtained. The flow field and extrusion shape of seven cross sections were compared and analyzed. And the flow chart of typical sections was compared in depth. The results showed that the flow velocity distribution in the die was more uniform. …

Biological Characteristics And Pathogenicity Of Botryosphaeria Parva Isolated From ‘Hongyang’ Kiwifruit, He Jingliu, Qin Wen, Liu Xiaoyan, Li Hongyi, Shen Dandan

Food and Machinery

Objects: In order to understand the biological characteristics of Botryosphaeria parva causing soft rot of ‘Hongyang’ kiwifruit. Methods: The mycelial structure was observed by optical microscopy and transmission electron microscopy. The growth curve was established with dry cell weight. Multiple methods could be induced to sporulate. The optimum growth condition of Botryosphaeria parva, and its relationship between lethal temperature and lethal time, as well as its pathogenicity in other fruits were studied. Results: There were branching, diaphragm and intact cell for the mycelium of Botryosphaeria parva. The growth curve of Botryosphaeria parva was divided into three periods, including lag phase …

Effect Of Hyaluronic Acid Coating On Fresh Quality Of Crucian Carp (Carassius Auratus) During The Partial-Freezing Storage, Guo Li, Wang Peng, Jiang Zhe, Guo Yanli, Zhou Fengchao, Chai Yunlei

Food and Machinery

The surface of Crucian carp (Carassius auratus) meat was coated with different concentrations of hyaluronic acid (HA) and stored at partial-freezing condition(-3 ℃) to study the effect of hyaluronic acid on its freshness preservation. The total volatile base-nitrogen (TVB-N), pH value, conductivity, thiobarbituric acid (TBA), water holding capacity, color and texture were measured as quality indexes to analyze the change of fish meat. The results showed that the meat quality of Crucian carp decreased slowly by combination of HA coating during the partial-freezing storage. The fish meat with 0.9% HA coating maintained its fresh quality on TVB-N (9.64 mg/100 g), …

Effects Of Leaf Crude Extracts From Gingo Biloba L. On The Preservation Of Litchi, Li Ling, Yan Xuyu, Chen Tiebi, He Fulin

Food and Machinery

The leaf crude extracts were obtained from Gingo biloba L. using ethanol extraction method, and its preservation on litchi was studied on the aspects of color, V_C content, weight loss rate, the total number of bacteria and sensory evaluation. The results showed that leaf crude extract of ginkgo biloba could reduce the loss of water, inhibit the browning, reduce the decrease of V_C content, the infection of litchi flesh and the decay rate of litchi, and keep the sensory quality of litchi. Its ability of antibacterial effects and preservation was followed by 0.6% leaf crude extract of ginkgo …

Effect Of Anti-Aging On Human Epidermal Keratinocyte By Uvb Induced For Green Tea Crude Water Extract, Wang Zhen, Liu Zhonghua, Cai Shuxian, Liu An, Wen Yi

Food and Machinery

In this study, anti-aging effects of green tea crude water extract were studied using human epidermal keratinocyte as model which induced by ultraviolet B (UVB). The green tea extract of 0～20 μg/mL was added to DMEM cell culture medium, then cells were induced by radiation of 60 mJ/cm² UVB. The anti-aging effects were studied by cellular morphology, apoptosis level, cell viability, intracellular antioxidant enzyme activity, intracellular active oxygen cluster (ROS) content, malonaldehyde（MDA）content, cell mitochondrial membrane potential and Na⁺,K⁺-ATP enzyme activities. The results showed that the crude extract of green tea could obviously improve cell volume, …

Interventional Effects Of Wild Oenanthe Javanica Extract In Guizhou Province In Type 2 Diabetic Mice, Chen Xiaojing, Guo Xiaoqing, Wu Nayiyu, Dai Tiantian

Food and Machinery

After 5 weeks feeding, the type 2 diabetes mellitus was established by intraperitoneal injection of low-dose STZ (75 mg/kg), to measure and statistically analyz the body weight, blood lipid level, fastig blood glucose level. The extracs of Oenanthe javanica can prevent the increase of blood glucose and the reduction of blood weight in STZ-induced type 2 diabets mice. In the study, the type 2 diabetic mice modle using high glucose and high fat diet and low dose STZ injection occurred at 3weeks, the achievment ratio was 80%, the aqueous extracts composition mold rate was 40%, the alcohol extracts was 60%. …

Functional Properties And Optimization Of Microwave Assisted Alkaline Extraction Of Dietary Fiber From Pomelo Peel, Yan Rongling, Liao Yang, Mao Longyi, He Fulin

Food and Machinery

The extraction technology of dietary fiber from Jiangyong pomelo peel by microwave assisted alkali method was established and the physicochemical properties and antioxidant activity of dietary fiber were analyzed. The results showed that, ensuring the extraction rate of soluble dietary fiber preferentially, the optimum extraction process of dietary fiber were solid-liquid ratio of 110 (g/mL), NaOH concentration of 8%, microwave time 40 s and microwave power 350 W. With the contrl of the optimal conditions, the maximum extraction yield rate of soluble dietary fiber (SDF) and insoluble dietary fiber (IDF) was 40.8% and 45.1%, respectively. Moreove, the dietary fiber showed …

Study On Microwave-Assisted Extraction And Antioxidant Activity Of Total Saponins From Chenopodium Quinoa Peel, Yang Jie, Gao Fengxiang, Yang Min, Bian Hongyu, Zhang Shigang, Zhao Erlao

Food and Machinery

The microwave-assisted extraction process of total saponin from Chenopodium quinoa peel of quinoa was optimized by one-factor combined response surface method with total saponin yield as index. The antioxidant activities were evaluated by DPPH free radical, hydroxyl radical and ABTS free radical method. The results showed that the ethanol concentration, microwave power and solid-liquid ratio had a significant effect on the total saponin yield. The optimum conditions were ethanol concentration 68%, microwave power 455 W, solid-liquid ratio 132 (g/mL), microwave time 10 min, and extraction times 2 times. The yield of total saponin from Chenopodium quinoa peel was 26.329 mg/g. …

Study On Separation And Purification Process Of Fisetin From Cotinus Coggygria Leaves With Polyamide, Xu Na, Xu Kaiyang, Fang Fang, Shu Xiaoshun, Li Chiling

Food and Machinery

To study the separation and purification of fisetin from Cotinus coggygria leaves with polyamide by using the adsorption rate and elution rate of fisetin as index. The effects of sample flow rate, sample concentration, concentration of eluent and dosage on purification process were investigated from static and dynamic adsorption. The optimum process conditions were as followed: the sample flow rate was 1 mL/min, the sample concentration was 0.535 8 mg/mL, the sample volume was 2 BV, the eluent was 70% ethanol, and the eluent dosage was 4 BV. Conclusion: The crude extract of Cotinus coggygria leaves was refined three times …

The Technology Optimization Of The Freezing Microwave Treatment Assisted Extraction Of Phenols From Red Grape Juice, Hong Meiling, Feng Zuoshan, Bai Yujia, Zheng Wancai, Ma Dexiu

Food and Machinery

In order to promote the rapid dissolution of anthocyanins and other polyphenols in colored grapes into grape juice, taking cabernet sauvignon grape as raw materia, using non thermal processing technology combined with freezing microwave treatment. Based on the single factor experiment, choosing the anthocyanin and polyphenol content of dual response value. Factors such as sample quality, freezing time, freezing temperature, microwave time and microwave power were selected as optimization factors, designed optimization of response surface methodology based on Box-Behnken experimental design principle. The optimum conditions for the simultaneous optimization of the two indicators were as follows: The sample quality was …

Study On Optimization And Effect Of Microwave Assisted Dilute Acid Degradation Of Corn Cob, Zhu Liyan, Yang Xiaorui, Wang Wei, Liang Jinhua, Zhu Jianliang

Food and Machinery

In order to improve the utilization rate and economic benefit of lignocellulose, the hydrolysis of corncob was studied by the method of using dilute acid as catalyst under microwave irradiation. Through single factor and orthogonal way ,the influences of microwave radiation time, microwave power, acid concentration, solid-liquid ratio and particle size of corncob were discussed with sugar yield and raw material conversion rate as index ; Raw materials and three methods of treatment of hydrolyzed residue were analyzed by scanning electron microscopy (SEM) and infrared spectroscopy (FT-IR).The results showed that the optimal reaction conditions were as follow: corncob was crushed …

Optimization On Mixed Fermentation Process With Brown Rice Ferment, Zhang Xupu, Bai Junyan, Liu Tengyun, Wu Rongrong, Cheng Shumei

Food and Machinery

The mixed fermentation process of enzymatic brown rice was optimized by the response surface method. In order to optimize the growth of Saccharomyces cerevisiae and Lactobacillus plantarum in short-term mixed fermented brown rice, the fermentation medium components of the traditional brown rice enzyme were optimized and the interaction between the constituent components was analyzed. Firstly, the main factors were analyzed by pre-experiemntal. The vital influencing factors of biomass, such as honey, brown rice and NaCl, were determined by Plackett-Burman test. The direction and step size of the steepest climbing test are set according to the three important factors effect intensity. …

Effect Of Microwave Drying Conditions On Volatile Oil Content In Zanthoxylum Schinifolium And Its Process Optimization, Peng Lin, Tian Bin, Wang Lin, Kan Jianquan, Chen Hourong

Food and Machinery

The Optimization of the microwave drying process was investigated using the response surface method in the “Jiuyeqing” Zanthoxylum schinifolium,with enzyme-inactivated by steam. The effects of microwave power, volume and intermittent microwave time on the content of volatile oil in microwave-dried Z. schinifolium were studied, and the suitable influence range was determined. On the basis of single factor experiment, the content of volatile oil was based on the Box-behnken Center combination test design principle, and the microwave drying regression model of it was established. The results showed that the optimal microwave drying parameters were as follows. The microwave power was 354.26 …

Investigating The Role Of Small Noncoding Rnas In Vertebrate Anoxia Tolerance, Claire Louise Riggs

Dissertations and Theses

Very few vertebrates survive extended periods of time without oxygen. Entry into metabolic depression is central to surviving anoxia, which is supported by overall suppression of protein synthesis, yet requires increased expression of specific proteins. Studying the rapid and complex regulation of gene expression associated with survival of anoxia may uncover new mechanisms of cellular biology and transform our understanding of cells, as well as inform prevention and treatment of heart attack and stroke in humans. Small non-coding RNAs (sncRNAs) have emerged as regulators of gene expression that can be rapidly employed, can target individual genes or suites of genes, …

Provitamin A Biofortification Of Cassava Enhances Shelf Life But Reduces Dry Matter Content Of Storage Roots Due To Altered Carbon Partitioning Into Starch, Getu Beyene, Felix R. Solomon, Raj D. Chauhan, Eliana Gaitán-Solis, Narayanan Narayanan, Jackson Gehan, Dimuth Siritunga, Robin L. Stevens, John Jifon, Joyce Van Eck, Edward Linsler, Malia Gehan, Muhammad Ilyas, Martin Fregene, Richard T. Sayre, Paul Anderson, Nigel Taylor, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Storage roots of cassava (Manihot esculenta Crantz), a major subsistence crop of sub-Saharan Africa, are calorie rich but deficient in essential micronutrients, including provitamin A β-carotene. In this study, β-carotene concentrations in cassava storage roots were enhanced by coexpression of transgenes for deoxy-d-xylulose-5-phosphate synthase (DXS) and bacterial phytoene synthase (crtB), mediated by the patatin-type 1 promoter. Storage roots harvested from field-grown plants accumulated carotenoids to ≤50 lg/g DW, 15- to 20-fold increases relative to roots from nontransgenic plants. Approximately 85%–90% of these carotenoids accumulated as all-trans-β-carotene, the most nutritionally efficacious carotenoid. β-Carotene-accumulating storage roots displayed delayed onset of postharvest …

Nuclear Spindles Pave The Way To Metastasis, Patrick J. Hensley, Natasha Kyprianou

Urology Faculty Publications

No abstract provided.