Life Sciences Commons^™

Evaluation Of A Field-Deployable Reverse Transcription-Insulated Isothermal Pcr For Rapid And Sensitive On-Site Detection Of Zika Virus, Mariano Carossino, Yanqiu Li, Pei-Yu A. Lee, Chuan-Fu Tsai, Pin-Hsing Chou, Dennis Williams, Ashley Skillman, R. Frank Cook, Grayson Brown, Hsiao-Fen G. Chang, Hwa-Tang T. Wang, Udeni B. R. Balasuriya

Maxwell H. Gluck Equine Research Center Faculty Publications

Background: The recent emergence of Zika virus (ZIKV) in Brazil and its precipitous expansion throughout the Americas has highlighted the urgent need for a rapid and reliable on-site diagnostic assay suitable for viral detection. Such point-of-need (PON), low-cost diagnostics are essential for ZIKV control in vulnerable areas with limited resources.

Methods: We developed and evaluated a ZIKV-specific field-deployable RT-iiPCR reagent set targeting the E gene for rapid detection of ZIKV in ZIKV-spiked human and mosquito specimens, and compared its performance to the Center for Disease Control and Prevention (CDC) and Pan American Health Organization (PAHO) RT-qPCR assays targeting the E …

Comparison Between Listeria Sensu Stricto And Listeria Sensu Lato Strains Identifies Novel Determinants Involved In Infection, Jakob Schardt, Grant Jones, Stefanie Müller-Herbst, Kristina Schauer, Sarah E. F. D'Orazio, Thilo M. Fuchs

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The human pathogen L. monocytogenes and the animal pathogen L. ivanovii, together with four other species isolated from symptom-free animals, form the "Listeria sensu stricto" clade. The members of the second clade, "Listeria sensu lato", are believed to be solely environmental bacteria without the ability to colonize mammalian hosts. To identify novel determinants that contribute to infection by L. monocytogenes, the causative agent of the foodborne disease listeriosis, we performed a genome comparison of the two clades and found 151 candidate genes that are conserved in the Listeria sensu stricto species. Two factors were …

Inhibition Of Growth And Ammonia Production Of Ruminal Hyper Ammonia-Producing Bacteria By Chinook Or Galena Hops After Long-Term Storage, Michael D. Flythe, Brittany E. Harlow, Glen E. Aiken, Gloria L. Gellin, Isabelle A. Kagan, Jesse Pappas

Animal and Food Sciences Faculty Publications

Surplus hops (Humulus lupulus L.) that are not needed by the brewing industry could be used as a feed supplement for cattle and other ruminants. Previous research indicates that antimicrobial hops plant secondary metabolites (i.e., α- and β-acids) inhibit methane and ammonia production and promote the growth of ruminant animals. The goal was to determine that hop pellets produced for brewing still possessed the requisite antimicrobial activity after 5-year storage. HPLC (high performance liquid chromatography) analysis indicated that the α- and β-acid concentrations in two varieties of hops were relatively stable after 5 years of storage under N_{2 …}

From Ground Pools To Treeholes: Convergent Evolution Of Habitat And Phenotype In Aedes Mosquitoes, John Soghigian, Theodore G. Andreadis, Todd P. Livdahl

Biology

Background: Invasive mosquito species are responsible for millions of vector-borne disease cases annually. The global invasive success of Aedes mosquitoes such as Aedes aegypti and Aedes albopictus has relied on the human transport of immature stages in container habitats. However, despite the importance of these mosquitoes and this ecological specialization to their widespread dispersal, evolution of habitat specialization in this group has remained largely unstudied. We use comparative methods to evaluate the evolution of habitat specialization and its potential influence on larval morphology, and evaluate whether container dwelling and invasiveness are monophyletic in Aedes. Results: We show that habitat specialization …

Population-Level Plasticity In Foraging Behavior Of Western Gulls (Larus Occidentalis), Scott A. Shaffer, Sue Cockerham, Pete Warzybok, Russell Bradley, Jaime Jahncke, Corey Clatterbuck, Magali Lucia, Jennifer Jelincic, Anne Cassell, Emma Kelsey, Josh Adams

Faculty Publications, Biological Sciences

Background Plasticity in foraging behavior among individuals, or across populations may reduce competition. As a generalist carnivore, western gulls (Larus occidentalis) consume a wide range of marine and terrestrial foods. However, the foraging patterns and habitat selection (ocean or land) of western gulls is not well understood, despite their ubiquity in coastal California. Here, we used GPS loggers to compare the foraging behavior and habitat use of western gulls breeding at two island colonies in central California. Results Gulls from offshore Southeast Farallon Island (SFI; n = 41 gulls) conducted more oceanic trips (n = 90) of …

Dna Base Excision Repair And Double Strand Break Repair In Human Fibroblast, Mingyang Li

LSU Doctoral Dissertations

In eukaryotes, DNA repair mechanisms detect and repair damaged DNA. DNA damage is primarily caused by a variety of exogenous and endogenous sources. Several types of damage to DNA are repaired by different kinds of DNA repair pathways. This dissertation focused on repair of N-methylpurines (NMPs) and double-strand breaks (DSBs) in human fibroblasts.

NMPs, including N⁷-methylguanine (7MeG) and N³-methyladenine (3MeA), can be induced by environmental methylating agents (e.g. the soil fumigant methyl bromide), chemotherapeutics (e.g. nitrogen mustards), and natural cellular methyl donors like S-adenosylmethionine. In human cells, NMPs are repaired by the multi-step …

Assessment Of Vegetation Response To Wildfire At Bandelier National Monument, New Mexico: Case Study Of The Las Conchas Fire, Gladys Valentin-Gonzalez

Geography ETDs

In the past couple of decades, there has been an increase in the occurrence of wildfire events in the United States. The U.S. Forest Service estimates that over 73,000 wildfires on average occur annually in the U.S., burning about 7.3 million acres of land. Bandelier National Monument, in northern New Mexico, has been affected by several wildfires in the past 40 years, one of the most recent being the Las Conchas Fire in 2011, which burned over 60,700 hectaresin Bandelier National Monument and Valles Caldera National Preserve. This research explores a remote-sensing based assessment of vegetation response to and recovery …

The Blurred Line Between Photic And Aphotic Environments: A Large Mexican Cave With Almost No Dark Zone, Luis M. Mejía-Ortíz, Tanja Pipan, David C. Culver, Peter Sprouse

International Journal of Speleology

Sistema Muévelo Rico is a 1.2 km long cave in Quintana Roo, less than 2 km from the Caribbean Sea. We measured illuminance to a level of 0.1 lux, organic matter (weight loss on ignition), temperature, and relative humidity. The last two were measured at hourly intervals for nearly one year. Approximately one-third of the cave has illuminance values greater than 0.01 lux, and most of the rest of the cave has light as well. Temperature and relative humidity were relatively constant, but they showed a daily cycle at all stations, albeit with different strengths. Organic matter averaged 8%, intermediate …

Testing The Underlying Chemical Principles Of The Biotic Ligand Model (Blm) To Marine Copper Systems: Measuring Copper Speciation Using Fluorescence Quenching, Tara N. Tait, James C. Mcgeer, Scott Smith

Chemistry Faculty Publications

Speciation of copper in marine systems strongly influences the ability of copper to cause toxicity. Natural organic matter (NOM) contains many binding sites which provides a protective effect on copper toxicity. The purpose of this study was to characterize copper binding with NOM using fluorescence quenching techniques. Fluorescence quenching of NOM with copper was performed on nine sea water samples. The resulting stability con- stants and binding capacities were consistent with literature values of marine NOM, show- ing strong binding with log K values from 7.64 to 10.2 and binding capacities ranging from 15 to 3110 nmole mg C ^{−1 …}

Philippine Aquatic Food Production Afs 450, Michael Cerbo

Library Impact Statements

No abstract provided.

Integrated Transcriptomic Analysis Of Trichosporon Asahii Uncovers The Core Genes And Pathways Of Fluconazole Resistance., Haitao Li, Congmin Wang, Yong Chen, Shaoqiang Zhang, Rongya Yang

College of Science & Mathematics Departmental Research

Trichosporon asahii (T. asahii) has emerged as a dangerous pathogen that causes rare but life-threatening infections. Its resistance to certain antifungal agents makes it difficult to treat, especially for patients undergoing long-term antibiotic therapy. In this study, we performed a series of fluconazole (FLC) perturbation experiments for two T. asahii strains, a clinical isolate stain CBS 2479 (T2) and an environmental isolate strain CBS 8904 (T8), to uncover potential genes and pathways involved in FLC resistance. We achieved 10 transcriptomes of T2 and T8 that were based on dose and time series of FLC perturbations. Systematic comparisons of the transcriptomes …

Synthesis And Characterisation Of Hydroxyl-Terminated Liquid Natural Rubber By Photo-Fenton Reaction, Pham H. L., Do B. T., Pham T. S., Le D. G.

ASEAN Journal on Science and Technology for Development

Hydroxyl-terminated liquid natural rubber (HTNR) was prepared by the photo-Fenton reaction, which was carried out in solution in the presence of H2O2/Fe(II) combined with UV irradiation. The HTNR obtained was characterized by GPC, FT-IR, 1H-NMR, 13C-NMR, DEPT-NMR spectroscopy as well as by chemical methods. A probable mechanism leading to the formation of HTNR was discussed based on the analytical data.

A Preliminary Study: Comparative Toxicity Of Extracts From Tinospora Tuberculata Beumee And Lumnitzera Racemosa Willd On Aedes Aegypti Linnaeus Larvae (Diptera: Culicidae), Wahizatul A. A., Shasita R.

ASEAN Journal on Science and Technology for Development

Research has been widely made on the potential use of herbal plants as effective agents for mosquito vector control. However, only a few studies have been carried out to investigate the potential insecticidal activity of mangrove plants against these dengue vectors. This study was carried out to examine the comparative toxicity of two mangrove plants, Tinospora tuberculata and Lumnitzera racemosa against the Aedes aegypti larvae. The leaf and stem of T. tuberculata and L. racemosa, were extracted using 95% methanol and these extracts bioassayed against the 3rd instar larvae of aegypti under laboratory conditions. The LC50 values of the crude …

Ticks, Ixodes Scapularis, Feed Repeatedly On White-Footed Mice Despite Strong Inflammatory Response: An Expanding Paradigm For Understanding Tick-Host Interactions, Jennifer M. Anderson, Ian N. Moore, Bianca M. Nagata, José M.C. Ribeiro, Jesus G. Valenzuela, Daniel E. Sonenshine

Biological Sciences Faculty Publications

Ticks transmit infectious agents including bacteria, viruses and protozoa. However, their transmission may be compromised by host resistance to repeated tick feeding. Increasing host resistance to repeated tick bites is well known in laboratory animals, including intense inflammation at the bite sites. However, it is not known whether this also occurs in wild rodents such as white-footed mice, Peromyscus leucopus, and other wildlife, or if it occurs at all. According to the "host immune incompetence" hypothesis, if these mice do not have a strong inflammatory response, they would not reject repeated tick bites by Ixodes scapularis. To test …

Performance Appraisal Of Non-Governmental Organizations: A Discussion On Pakistan 2010 Floods Response And Recovery, Ali A., Baig N., Khan A.

ASEAN Journal on Science and Technology for Development

The paper examines in detail the role of NGOs (Non-governmental Organizations) in response and recovery phase of a disaster. Donors, INGOs (International Non-governmental Organizations) and NGOs work for the betterment of affected community but due to certain shortfalls, the community did not benefit as much as intended. In this research those gray areas are highlighted in detail through field visits and interaction with the community devastated by 2010 floods. Lack of inter-agency cooperation, partnership with inexperienced local NGOs and lack of transparency and accountability mechanisms are those areas which reduce the effectiveness of response and recovery efforts. These gray areas …

Computational Fluid Dynamics Simulations Of Gas-Liquid Two-Phase Flow Characteristics Through A Vertical To Horizontal Right Angled Elbow, Aung N. Z., Yuwono T.

ASEAN Journal on Science and Technology for Development

Having a clear understanding on the phase distribution of gas-liquid two-phase flow through elbow bends is vital in mixing and separation system designs. This paper presents the computational fluid dynamics (CFD) simulations and experimental observations of gas-liquid two-phase flow pattern characteristic through a vertical to horizontal right angled (90°) elbow. Experimental observations were conducted in a transparent test section that consisted of a vertical pipe, elbow bend and horizontal pipe with an inside diameter of 0.036 m. The CFD simulations were performed by using a computer software package, FLUENT 6.2. Bubbly flow conditions were created in the vertical test section …

Oceanic, Latitudinal, And Sex-Specific Variation In Demography Of A Tropical Deepwater Snapper Across The Indo-Pacific Region, Ashley J. Williams, C. Wakefield, Stephen J. Newman, Elodie Vourey, Francisco J. Abascal, Tuikolongahau Halafihi, Jeremie Kaltavara, Simon J. Nicol

Fisheries Research Articles

Deepwater tropical fisheries provide an important source of income and protein to Pacific and Indian Ocean coastal communities who are highly dependent on fish for food security. The development of quantitative assessments and management strategies for these deepwater fisheries has been hindered by insufficient biological and fisheries data. We examine the age-specific demography of the pygmy ruby snapper Etelis carbunculus, an important target species in tropical deepwater fisheries, across 90° of longitude and 20° of latitude in the Pacific and Indian Oceans. Our results show that growth of E. carbunculus varies significantly between oceans and sexes and across latitudes …

Daphnia: A Possible Way To Combat A Deadly Amphibian Pathogen, Taylor Thomas

Undergraduate Honors Theses

Globally there is a biodiversity crisis, with many groups of species threatened with extinction due to changes in the environment and human impacts. Amphibians are one such group and according to the IUCN, over 30% of amphibians are threatened by extinction. There are many factors have that can explain the decline of amphibians including pollution, habitat loss, climate change and disease. One factor is chytridiomycosis, an emerging infectious disease caused by the chytrid Batrachochytrium dendrobatidis. The chytrid enters the keratinized skin of the amphibian and asexually reproduces, where it disrupts host functions, often leading to host death.

Due to …

Biodegradation Of Nr Latex-Based Materials Via A Carbon Dioxide Evolution Method, Shabinah F. M. S., Hashim M. Y. A.

ASEAN Journal on Science and Technology for Development

NR as a natural polymer has biodegradable characteristics and their existence was examined using CO2 evolution methods. The CO2 molecule produced by micro-organism metabolisms in the degradation system was quantified using a conventional acidimetric method. An aerobic system was determined as most suitable condition to be examined under this method. The presence of O2 in the system would help micro-organisms to destabilize the natural polymer. The material of LATZ, HA film and NR gloves showed significant weight loss and were able to produce CO2 evolution curves after 45 days in the biodegradation system compared to synthetic polyisoprene films. Gel permeation …

Effects Of Chocolates Using Low Calorie Cocoa Butter Substitutes On Rat’S Plasma Profile And Determination Of Sn-1,3 Position, Ros-Haniza B.., Mamot S.

ASEAN Journal on Science and Technology for Development

The present study sought to determine the effectiveness of newly developed low calorie chocolate using modified fat through enzymatic interesterification process as cocoa butter substitute of male Sprague Dawley rats (n = 35) compared to cocoa butter as control. Body weight gains did not differ significantly (p>0.05) in all the test groups fed with chocolates which were using different types of fats. Triacylglycerol and cholesterol test showed no significant difference (p>0.05) for all treatment samples. in this study, a pancreatic lipase hydrolysis was performed to determine the fatty acid composition of the sn-2 position of the structure lipids.

A Gut Pathobiont Synergizes With The Microbiota To Instigate Inflammatory Disease Marked By Immunoreactivity Against Other Symbionts But Not Itself, João Carlos Gomes-Neto, Hatem Kittana, Sara Mantz, Rafael R. Segura Munoz, Robert J. Schmaltz, Laure B. Bindels, Jennifer L. Clarke, Jesse M. Hostetter, Andrew K. Benson, Jens Walter, Amanda Ramer-Tait

Department of Food Science and Technology: Faculty Publications

Inflammatory bowel diseases (IBD) are likely driven by aberrant immune responses directed against the resident microbiota. Although IBD is commonly associated with a dysbiotic microbiota enriched in putative pathobionts, the etiological agents of IBD remain unknown. Using a pathobiont-induced intestinal inflammation model and a defined bacterial community, we provide new insights into the immune-microbiota interactions during disease. In this model system, the pathobiont Helicobacter bilis instigates disease following sub-pathological dextran sulfate sodium treatment. We show that H. bilis causes mild inflammation in mono-associated mice, but severe disease in the presence of a microbiota, demonstrating synergy between the pathobiont and microbiota …

Geographic Variation In Juniperus Drupacea: Dna Sequencing And Volatile Leaf Oils: Further Evidence Of Putative Pleistocene Genetic Isolation Between Europe And Asia, Robert P. Adams, Adam Boratynski, Magda Dou Dagher-Kharrat, Hagar V. Leschner, Tuğrul Mataraci, Andrea E. Schwarzbach

Health & Biomedical Sciences Faculty Publications

Recently, Sobierajska et al. (2016), using nSSR and morphology, showed that Juniperus drupacea exhibited differentiation between Greece and Turkey/ Lebanon, suggestive of Pleistocene genetic isolation. Here, we report that leaf terpenoids and DNA sequence data support their hypothesis by confirming differentiation between Greece and Turkey/ Lebanon/ Israel. The leaf oils of the Turkey/ Lebanon plants contained one unique terpene (trans-verbenol, 0.1-1.4%) that was absent in the Greece plants. The Greece oil contained three terpenes not found in the Lebanon/ Turkey plants: (ar)-curcumene (2.2%), β-alaskene (0.3%) and α-alaskene (0.4%). Four other terpenes were in higher concentration in the Greece oils: camphene …

Toughening Of Bisphenol-A Diglycidyl Ether-Based Epoxy By Modification With Hydroxyl-Terminated Liquid Natural Rubber, Pham H. L., Do B. T., Pham T. S., Le D. G.

ASEAN Journal on Science and Technology for Development

Hydroxyl-terminated liquid natural rubbers (HTNRs), prepared by the Photo-Fenton reaction, were used to modify bisphenol-A diglycidyl ether-based epoxy (DGEBA). A chemical link between HTNRs and the epoxy resin was promoted employing toluene diisocyanate. The reactions between elastomers and epoxy resin were followed by FTIR. The mechanical properties of the composites were evaluated and the microstructure was investigated using scanning electronic microscopy. The results showed that the impact resistance of HTNR-modified DGEBA was superior to that of the pure epoxy resin. For the composites with HTNR, the impact resistance increased with elastomer concentration up to 2.5 parts per hundred parts of …

Physical Fitness And Metabolic Profile Among Malay Undergraduates Of A Public University In Selangor Malaysia, Emad M., Kandiah M., Lim W. K., Barakatun-Nisak M. Y., Rahmat A., Norasruddin S., Appukutty M.

ASEAN Journal on Science and Technology for Development

This study investigated health-related components of physical fitness consisting of morphological fitness (body fat % or BF %; Body Mass Index or BMI; and waist circumference or WC), metabolic fitness (blood glucose, lipid profiles and haemoglobin) and aerobic capacity (VO2max). This crosssectional study involved 324 undergraduates recruited voluntarily by systematic random sampling from a public university in the city Shah Alam, Selangor Malaysia. The respondents’ aerobic capacity was measured by field fitness tests and anthropometric measurements using standard protocols. The mean BMI of respondents was 22.51 ± 4.18 kg/m2, and majority of the respondents (93%) are within normal range of …

Beverly Cross, Beverly Cross, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

One of the first hires for the new National Food Service Management Institute/Institute of Child Nutrition, Beverly Cross spent more than a quarter of a century working for the Institute. Here she shares her reflections as she prepares for her retirement in December 2017.

Integrating Herbivore Population Dynamics Into A Global Land Biosphere Model: Plugging Animals Into The Earth System, Shree R. S. Dangal, Hanqin Tian, Chaoqun Lu, Wei Ren, Shufen Pan, Jia Yang, Nicola Di Cosmo, Amy Hessl

Plant and Soil Sciences Faculty Publications

Mammalian herbivores are an essential component of grassland and savanna ecosystems, and with feedbacks to the climate system. To date, the response and feedbacks of mammalian herbivores to changes in both abiotic and biotic factors are poorly quantified and not adequately represented in the current global land surface modeling framework. In this study, we coupled herbivore population dynamics in a global land model (the Dynamic Land Ecosystem Model, DLEM 3.0) to simulate populations of horses, cattle, sheep, and goats, and their responses to changes in multiple environmental factors at the site level across different continents during 1980–2010. Simulated results show …

Pengaruh Pola Tanam Tumpangsari Jagung Dan Kedelai Terhadap Pertumbuhan Dan Hasil Jagung Hibrida Dan Evaluasi Tumpangsari Di Arjasari Kabupaten Bandung, Yuyun Yuwariah, Dedi Ruswandi, Aep Wawan Irwan

Jurnal Kultivasi

Jagung dan kedelai merupakan tanaman pangan terpenting setelah padi di Indonesia. Konsumsi jagung dan kedelai akan terus mengalami peningkatan setiap tahun dikarenakan pertambahan jumlah penduduk. Salah satu upaya untuk meningkatkan produksi tanaman dapat dilakukan dengan cara tumpangsari. Sistem tumpangsari merupakan sistem pertanaman dengan menanam dua atau lebih jenis tanaman secara serentak pada lahan yang sama dalam waktu satu tahun. Penelitian bertujuan untuk mengetahui genotip jagung hibrida yang terbaik ditumpangsarikan dengan kedelai. Percobaan dilaksanakan dari bulan Maret 2016 sampai bulan Agustus 2016 di Arjasari, Kabupaten Bandung dengan ketinggian tempat mencapai 960 m di atas permukaan laut. Rancangan yang digunakan dalam penelitian …

Respons Jagung (Zea Mays L.) Akibat Jarak Tanam Pada Sistem Tanam Legowo (2:1) Dan Berbagai Dosis Pupuk Nitrogen Pada Tanah Inceptisol Jatinangor, Agus Wahyudin, Yuyun Yuwariah, Fiky Yulianto Wicaksono, Reza Aed Galib Bajri

Jurnal Kultivasi

Di Indonesia jagung menjadi sumber karbohidrat kedua setelah padi. Selain sebagai sumber kebutuhan pangan jagung juga dapat digunakan sebagai sumber pakan ternak dan bahan baku industri.Penelitian ini bertujuan untuk mengamatiinteraksi sistem tanam legowo (2:1) dengan pupuk N yang menghasilkan pertum-buhan dan hasil terbaik pada tanaman jagung (Zea mays L.) Hibrida Pertiwi 3, di Jatinangor mulai bulan November 2016 sampai Maret 2017. Percobaan menggunakan Rancangan Acak Kelompok Pola Faktorial. Perlakuan terdiri dari dua faktor, masing-masing faktor terdiri dari tiga taraf dan diulang tiga kali. Faktor pertama adalah sistem tanam legowo (2:1) dengan menggunakan jarak tanam yang terdiri dari tiga taraf yaitu …

Hasil Benih Empat Kultivar Kedelai Yang Ditanam Di Dataran Medium Dan Dataran Tinggi, Sumadi, Muhamad Kadapi, Anne Nuraini, Noladhi Wicaksana, Meddy Rachmadi, S Rodiah

Jurnal Kultivasi

Pertumbuhan dan hasil suatu tanaman merupakan hasil interaksi antara karakter genetik setiap kultivar dengan lingkungan tumbuhnya. Tujuan penelitian ini adalah menetapkan kultivar kedelai yang paling cocok ditanam pada musim hujan di dataran medium ( ± 753 m dpl) dan dataran tinggi ( ± 1200 m dpl). Penelitian merupakan percobaan lapangan dilaksanakan pada April sampai dengan Juli 2016. Percobaan dirancang dalam Rancangan Acak Kelompok sederhana yang menguji kultivar meliputi Anjasmoro, Argomulyo, Arjasari dan Grobogan yang diulang 5 (lima) kali. Hasil percobaan menunjukkan bahwa secara umum potensi hasil kedelai di dataran medium hasilnya lebih tingggi dibandingkan yang ditanam di dataran tinggi. Kultivar …

Respons Tiga Klon Tanaman Rami (Boehmeria Nivea (L.) Gaud) Terhadap Konsentrasi Asam Giberelat Yang Berbeda, Cucu Suherman, Anne Nuraini, Veronika Sri Rahayu Wulandari

Jurnal Kultivasi

Tanaman rami merupakan salah satu jenis tanaman perkebunan penghasil serat yang serat-nya dapat dimanfaatkan dalam industri tekstil. Penelitian ini bertujuan untuk mengetahui pengaruh interaksi zat pengatur tumbuh asam giberelat (GA3) dan tiga klon rami terhadap pertumbuhan dan hasil tanaman rami serta mengetahui interaksi konsentrasi asam giberelat dan klon rami yang paling baik. Percobaan dilakukan di Kebun Percobaan Ciparanje, Fakultas Pertanian, Universitas Padjadjaran, Kabupaten Sumedang, ordo tanah Inceptisol, waktu pelaksanaan bulan Februari 2017 sampai bulan April 2017. Tipe curah hujan C (agak basah) menurut klasifikasi Schmidt dan Fergusson, dengan ketinggian tempat 754 mdpl. Percobaan ini menggunakan Rancangan Acak Kelompok pola Faktorial …