Examining The Regulation Of Kv7 K+ Channels In Airway Smooth Muscle Cells And Their Potential As Novel Therapeutic Targets For The Treatment Of Asthma, Jennifer Haick

Dissertations

Asthma is a disease characterized by nonspecific and exaggerated airway narrowing, termed airway hyperresponsiveness (AHR), which involves the excessive contraction of airway smooth muscle. Despite the fact that airway smooth muscle is widely studied and understood to play a role in AHR, little is known about the specifics of that role. Our laboratory recently found that Kv7 potassium (K+) channels are expressed in airway smooth muscle cells (ASMCs). Kv7 channels are voltage sensitive K+ channels whose outward flux of K+ ions promotes a negative resting membrane voltage in excitable cells, thereby opposing electrical excitability. Inhibition of K+ channels is known …

Role Of The Interleukin-22 And Stat3 Signaling Pathway In Gut Barrier Maintenance Following Intoxication And Burn Injury, Adam M. Hammer

Dissertations

More than half of patients admitted to burn centers for treatment have measurable alcohol levels in their blood. Intoxication that precedes a traumatic burn injury leads to worsened patient outcomes, including higher rates of infection and sepsis. The intestines harbor the largest concentration of bacteria in the human host. Any disruption of the intestinal barrier as a result of intoxication and burn injury could contribute to infection and/or sepsis from gut-derived microbes. Previous work has demonstrated that the intestinal barrier becomes leaky, and that administration of interleukin-22 (IL-22) significantly reduces gut barrier leakiness following alcohol and burn injury. However, the …

Endocytic Vesicle Rupture In The Pathogenesis And Propagation Of Neurodegenerative Proteinopathies, William P. Flavin

Dissertations

Numerous pathological amyloid proteins spread from cell-to-cell during neurodegenerative disease, facilitating the propagation of cellular pathology and disease progression. Understanding the mechanism by which disease-associated amyloid protein assemblies enter target cells and induce cellular dysfunction is therefore key to understanding the progressive nature of such neurodegenerative proteinopathies. In this study, we utilized an imaging-based assay to monitor the ability of disease-associated amyloid assemblies to induce the rupture of intracellular vesicles following endocytosis, as well as to elucidate the cellular consequences of this damaging mechanism of invasion. We observed that the ability to induce vesicle rupture is a conserved feature of …

Behavioral Symptoms, Functional And Cognitive Recovery Post-Mild Traumatic Brain Injury, Seema Nasser

Dissertations

Mild traumatic brain injury (MTBI) accounts for 80–90% of the nearly two million traumatic brain injuries (TBI) that occur each year. The psychological consequences of MTBI can be extensive and can persist well beyond the acute injury, profoundly impacting the lives of the individual, their families, and society. A substantial number of MTBI patients suffer depressive mood, fatigue, and poor sleep (i.e., behavioral symptoms) for weeks and months post-injury. These symptoms reduce quality of life and delay the return to previous cognitive and functional status. Behavioral symptoms of depressed mood, fatigue, and poor sleep commonly co-occur and thus may constitute …

Characterization Of Quorum-Quenching Lactonases And Plp-Dependent Aminotransferases: Structure, Mechanism And Alternative Turnover, Romila Nina Mascarenhas

Dissertations

Cell-to-cell communication by bacteria is essential for the regulation of gene expression important in colonization, biofilm formation, virulence and other processes. This communication is called "quorum-sensing" and is mediated by small molecules called autoinducers. One major class of autoinducers used by gram-negative bacteria is N-acyl homoserine lactones (AHL's). Enzymes capable of disrupting this communication are called "quorum-quenching" catalysts and have proven to be invaluable biochemical tools for understanding quorum-sensing pathways. Quorum-quenching enzymes hold promise for application in anti-biofouling, agriculture, aquaculture, bioremediation and other synthetic biology settings. However, the mechanisms that these enzymes use to recognize and process their substrates are …

Exploring The Impact Of Collective Bargaining Agreements On Employee Performance Management, Nana Gyesie

Walden Dissertations and Doctoral Studies

As of 2014, union density in the United States had dropped compared to union density during the 1950s. Collective bargaining agreements are the foundational agreement for all issues related to salary, benefits, and working conditions. The purpose of this multiple case study was to explore how collective bargaining agreements hindered or enabled managers from creating and sustaining high performance work practices. The conceptual framework included Walton and McKersie's work on behavioral theories for labor negotiations, human capital, and collective bargaining, and Huselid's work on high performance work practices. Fifteen respondents across 5 labor unions in Washington DC were selected through …

Identification, Diagnosis, Counseling, And Referral Of Overweight Military Dependent Children To Reverse Early Childhood Obesity, Gerald William Hall

Walden Dissertations and Doctoral Studies

Since 1980, the obesity rate in children 5 to 11 years of age has increased from 7% to 18%. The lack of structured physical activity and poor dietary habits childhood are primary risk factors for obesity related comorbidities in adulthood. Guided by primary care providers, families can reverse childhood obesity by implementing healthy dietary habits and engaging in structured physical activity. The purpose of this quality improvement project was to develop an evidenced-based policy with procedures to standardize the timely and consistent identification of overweight children at a primary care clinic serving military families. With an emphasis on obesity prevention …

Roles Of Community Pharmacists In Improving Oral Health Awareness In Plateau State, Northern Nigeria, Olaniyi O. Taiwo

Walden Dissertations and Doctoral Studies

There is poor oral health awareness in Nigeria. This is mainly attributed to limited access to correct information on oral health as well as a lack of oral health care providers. The impact of the poor oral health awareness is worse in Northern Nigeria due to the uneven distribution of oral health care workers and training facilities. The purpose of this cross-sectional study was to describe the roles of community pharmacists (CPs) in Plateau State, Nigeria as sources of oral health information. Theoretical framework for this study was the theory of planned behavior (TPB). Background knowledge and practices of oral …

Effect Of The National Accelerated Agricultural Inputs Access Subsidy Program On Fertilizer Usage And Food Production In Kakamega County, Western Kenya, Abednego Kiwia Mavuthu

Walden Dissertations and Doctoral Studies

Despite 25 years of concerted efforts by African governments to adopt consistent policies for increasing food production, hunger and poverty are still prevalent in the continent. Using Bernanke's conceptualization of the credit channel theory of monetary policy, the purpose of this correlational study was to investigate whether a subsidy program, the National Accelerated Agricultural Inputs Access Program (NAAIAP), affected the rates of fertilizer usage and food production in Kakamega County, Western Kenya. Purposive stratified sampling was used to select 114 participants consisting of 72 farmers in each of the 2 groups: NAAIAP beneficiaries and nonbeneficiaries. Participants completed a survey on …

Paths To Tier 1 Genomics Implementation: A Survey Of Chronic Disease Directors, Amy Ponte

Walden Dissertations and Doctoral Studies

Although evidence is currently available for population-based genetic screening and testing of individuals and their family members for certain hereditary chronic disease conditions (Tier 1), few states have integrated these genomic applications into chronic disease prevention programs. State and territorial chronic disease directors (CDDs) could provide the leadership needed to deliver these applications in more states. The purpose of this study was to determine whether an association exists between current chronic disease genomics funding or specific state genomic activities and the level of knowledge and interests in genomics by these directors. Rogers's diffusion of innovations (DIT) theory was used to …

Breast Cancer, Mana'olana/Hope, And The Experience Of Native Hawaiian Women, Karla Marie Calumet

Walden Dissertations and Doctoral Studies

Breast cancer is one of the leading causes of morbidity and mortality among women. A diagnosis of cancer is a stressful event that requires an individual to adapt to new stressors. The purpose of this qualitative study was to better understand the perceptions of mana'olana/hope and living with breast cancer among Native Hawaiian women. The conceptual framework of this phenomenological study was positive psychology. Data collection included in-depth interviews with 5 Native Hawaiian women who had been diagnosed with breast cancer. Data coding and analysis resulted in identification of 8 themes.

The themes included: (a) mana'olana /hope is the essence …

Strategies For Preventing And Mitigating The Effects Of Agro-Food Supply Chain Disruptions, Everjoyce Muzvondiwa

Walden Dissertations and Doctoral Studies

Supply chain disruptions are detrimental to the performance of companies due to the associated loss of profitability and reduced sustainability. In 2016, organizations lost at least $1.2 million in a single supply chain disruption. Guided by the contingency theory of fit, the purpose of this exploratory multiple case study was to explore the strategies agribusiness managers use to prevent and mitigate the effects of disruptions in the agro-food supply chains. A total of 5 purposefully-selected agribusiness managers from Harare, Zimbabwe participated in semistructured interviews. Participants were senior agribusiness managers who implemented successful strategies for preventing and mitigating the effects of …

The Association Between Dietary Flavonoid Intake And Chronic Obstructive Pulmonary Disease, Systemic Inflammation, And Comorbidity Burden, Tennador Sanderson

Walden Dissertations and Doctoral Studies

Chronic obstructive pulmonary disease (COPD) is a leading cause of morbidity and mortality in the United States. Dietary habits may have an impact on COPD through anti-inflammatory mechanisms. For this cross-sectional secondary data analysis study, the advanced model of the epidemiology triangle was used as a guide to assess the association between total daily flavonoid intake and COPD diagnosis, COPD severity, systemic inflammation, and comorbidity burden. Data from over 5,100 male and female participants aged 30 and older were obtained from the 2007-2010 National Health and Nutrition Examination Survey. The prevalence of COPD was 5.2% according to spirometry data. Multiple …

Instructional Strategies That Promote Cultural Competence In Nutrition And Dietetics Education, Cecile Adkins

Walden Dissertations and Doctoral Studies

Changing demographics in the United States to a minority-majority culture require health care professionals who are culturally competent to provide appropriate care to patients. In a university in the Mid-Atlantic region of the United States, a gap existed between student education and the culturally competent professional practice of entry-level registered dietitians. Using Freire's notion of social justice and Vygotsky's constructivist theory as the conceptual frameworks, the purpose of this study was to explore educators' instructional strategies that promoted cultural competence in nutrition and dietetics education. The research questions examined how educators described the instructional practices they employed to promote cultural …

Food Waste Reduction Strategies In Supermarkets: The Lived Experiences Of Perishable Food Managers In Michigan, Kelly K. Zimmermann

Walden Dissertations and Doctoral Studies

At the retail level, 25% of sellable food is wasted, and perishable foods account for 72% of food waste in the United States. The purpose of this qualitative phenomenological study was to explore the food waste reduction strategies used by supermarket perishable food department managers, which are covered under the Good Samaritan Food Donation Acts (state and federal). The participant population was comprised of 17 managers who are responsible for perishable foods departments (bakery, deli, meat, dairy, and produce) at local, regional, and national supermarkets in Midland, Michigan. Goal systems theory served as the conceptual framework lens for which the …

Education And The Development Of On-Farm Identity: Dairy Farm Women's Experiences, Amy Leigh Spangenberg

Walden Dissertations and Doctoral Studies

Women throughout the world have found emancipation through education. Yet, there has been no research on the role that education plays in dairy farm women's primary, on-farm identity development and how farm women's identities develop. The purpose of this phenomenological study was to examine dairy farm women's perceptions of previous learning (education) and its influence on their primary, on-farm identity development. The feminist standpoint theory provided the framework for this study. A homogeneous, purposeful sample of 10 active dairy farm women were interviewed using individual, face to face, semistructured questions to answer the question: What are dairy farm women's perceptions …

Predictors Of Compliance With The Food Safety And Inspection Service's Listeria Rule, 2012-2015, Amadou Samb

Walden Dissertations and Doctoral Studies

Since 1987, the Food Safety and Inspection Service (FSIS) has implemented a zero-tolerance policy for Listeria monocytogenes (Lm) in ready-to-eat (RTE) meat and poultry products, which culminated with the implementation of the Listeria rule in 2003. While researchers have extensively examined human listeriosis and its causative agent, Lm, there remained a significant gap in the current literature regarding how, singly or in combination, establishment size, RTE product type, Listeria alternative used, and FSIS district of production predict compliance with the Listeria rule. Therefore, the purpose of this quantitative study was to investigate the relationship between establishment size, RTE product type, …

Evolution Of The Kinetics And Dynamics Of Heme-Crevice Loop Regulating Chemistry In Human Cytochrome C, Shiloh M. Nold

Graduate Student Theses, Dissertations, & Professional Papers

Cytochrome c, cytc, is a metalloprotein that plays primary roles in electron transport and intrinsic apoptotic pathways. Much of the chemistry that cytc is involved with is regulated by a highly conserved region known as the heme crevice loop, consisting of residues 70-85. Only three of these residues (those at positions 81, 83 and 85) are not universally conserved within the evolutionary timeline. Here I look to elucidate possible evolutionary roles for several of the key residues known to be important in regulating heme chemistry of cytc.

I first address the role that lysine …

Evolution Of Molt Phenology In Dwarf Hamsters, Zachary Clare-Salzler

Graduate Student Theses, Dissertations, & Professional Papers

Environmental seasonality presents many challenges for survival. In response, organisms from across the tree of life exhibit adaptive phenology, which can facilitate survival from season to season. In particular, many species are white in the winter and brown in the summer where winter snow cover alters the visual environment. There is an extensive body of literature describing the physiological and cellular regulation of this trait in the Siberian dwarf hamster (Phodopus sungorus), but there are major shortcomings in our understanding concerning the evolution and molecular regulation of mammalian skin phenology. Notably, little is known about the genetic basis …

Contributions Of Gene Copy Number Variation To Genome Evolution And Local Adaptation Of The Cyanobacterium Acaryochloris, Amy L. Gallagher

Graduate Student Theses, Dissertations, & Professional Papers

Acaryochloris is a recently discovered genus of cyanobacteria, unique in its use of an uncommon chlorophyll as its major photosynthetic pigment, and in its peculiar genome dynamics. Members of this genus exhibit increased genic copy number variation (CNV), which is thought to be primarily derived from gene duplications and horizontal gene transfer (HGT). Acaryochloris provides an ideal system to explore mechanisms behind maintenance of gene duplicates and the influence of CNV in local adaptation. Here, I propose a mechanism for retention of gene duplicates of the bacterial recombinase, RecA, in Acaryochloris genomes and provide preliminary evidence that these paralogs are …

K-Mer Analysis Pipeline For Classification Of Dna Sequences From Metagenomic Samples, Russell Kaehler

Graduate Student Theses, Dissertations, & Professional Papers

Biological sequence datasets are increasing at a prodigious rate. The volume of data in these datasets surpasses what is observed in many other fields of science. New developments wherein metagenomic DNA from complex bacterial communities is recovered and sequenced are producing a new kind of data known as metagenomic data, which is comprised of DNA fragments from many genomes. Developing a utility to analyze such metagenomic data and predict the sample class from which it originated has many possible implications for ecological and medical applications. Within this document is a description of a series of analytical techniques used to process …

Molecular Diversity Of Foliar Fungal Endophytes In Relation To Defense Strategies And Disease In Whitebark Pine, Lorinda Bullington

Graduate Student Theses, Dissertations, & Professional Papers

An invasive fungal pathogen, Cronartium ribicola (the causative agent of white pine blister rust) infects and kills whitebark pine (Pinus albicaulis) throughout the western US. Blister rust has decreased whitebark pine populations by over 90% in some areas. Whitebark pine, a keystone species, has been proposed for listing under the Endangered Species Act in the U.S., and the loss of this conifer is predicted to have severe impacts on forest composition and function in high elevations. Hundreds of asymptomatic fungal species live inside whitebark pine tissue, and recent studies suggest that these fungi can influence the frequency and …

Harvest And Persistence Of Wolf Populations: Variable Effects Of Harvest On Wolf Packs In The Rocky Mountains, Sarah B. Bassing

Graduate Student Theses, Dissertations, & Professional Papers

Pubic harvest is a common method used to manage populations of wolves (Canis lupus) in North America. Although wolves appear resilient to the effects of harvest management the influences on demography and pack stability are uncertain. Packs generally drive population dynamics for wolves; thus, we were interested in how harvested populations were maintained and how harvest influenced the abundance and distribution of packs. We used noninvasive genetic data collected in Idaho, USA (2008–2014) and Alberta, Canada (2012–2014) to test whether immigration compensated for harvest mortality and helped maintain population densities. We further fit occupancy models to detection data …

Drivers And Feedbacks Of The Fire-Grazing Interaction In The Northern Great Plains, Jacob E. Powell

Graduate Student Theses, Dissertations, & Professional Papers

The fire-grazing interaction is well studied in mesic grasslands worldwide, but research is lacking in semiarid systems. In addition, the fire-grazing interaction reduces the invasion of exotic forage species in mesic grasslands by increasing the scale of grazing selection and may be a tool to control invasive plants in other grasslands. We examined the principal drivers and feedbacks of the fire-grazing interaction on the strength of cattle grazing selection, forage quantity and quality, and vegetation structure and composition in two pastures in northeast Montana at The Nature Conservancy’s Matador Ranch. We also determined the influence of time since fire, within …

Coupling Biophysical Complexity And Forest Metabolism In A Floodplain Landscape, Peter Davis

Graduate Student Theses, Dissertations, & Professional Papers

Floodplains are biophysically complex systems that are considered among the most productive and biodiverse ecosystems on Earth. Until recently, quantitative assessment of the relationship between complexity and terrestrial production has been constrained by technological limitation. To address how floodplain biophysical complexity and ecosystem function are related, I employed remote sensing, GIS, and spatial analyses to quantify and couple metrics of complexity and terrestrial production, as well as explore the relationship among complexity, vegetation structural diversity, and terrestrial primary productivity. The study site is a 6.75-km by 1.75-km portion of the Bitterroot River floodplain near Carlton, MT upon which 551 sample …

Understanding Patterns And Drivers Of Alaskan Fire-Regime Variability Across Spatial And Temporal Scales, Tyler J. Hoecker

Graduate Student Theses, Dissertations, & Professional Papers

Boreal forest and tundra ecosystems are globally important because the mobilization of large carbon stocks, and changes in energy balance could act as positive feedbacks to ongoing climate warming. In Alaska, wildfire is a key driver of ecosystem structure and function, and therefore fire strongly determines the feedbacks between high-latitude ecosystems and the larger Earth system. The paleoecological record from Alaska reveals the sensitivity of fire regimes to climatic and vegetation change over centennial to millennial time scales, highlighting increased burning with warming and/or increased landscape flammability associated with large-scale vegetation changes. This thesis focuses on two studies aimed at …

Does Timing Of Herbicide Use Influence Rates Of Germination Or Seedling Biomass Of Native Plants Used For Restoration?, Christine Mcmanamen

Graduate Student Theses, Dissertations, & Professional Papers

Invasive plants can negatively impact native grasslands by changing their species composition, productivity, and function. Managers commonly use herbicides as a control method; however, this practice can lead to secondary invasion by other non-native invasive plants, unless measures are taken to promote natives. Because of this, managers often seed native plants after spraying herbicides. There is evidence, however, that chemical control of invasive plants may reduce the effectiveness of subsequent seed-addition treatments, but there is currently little quantitative information on optimal timing between spraying and seeding or on variation in herbicide sensitivity among native plants commonly used in seed mixes. …

High-Resolution Mapping Of Hierarchical Greater Sage-Grouse Nesting Habitat: A Grain-Spectrum Approach In Northwestern Wyoming, Robert T. Haynam Iii

Graduate Student Theses, Dissertations, & Professional Papers

Our overall objective was to create a probabilistic nesting-habitat map for the Jackson Hole sage-grouse population that would have utility as a tool for future research, conservation, and management. The models that we developed for this purpose were specified to evaluate whether sage-grouse may be selecting nesting-habitat characteristics simultaneously at various spatial scales. Our spatially-explicit landscape-scale research was implemented primarily with readily available National Agriculture Imagery Program (NAIP) data. All nesting data was collected from 2007-2010. We tested how a broad range of grain sizes (spatial resolution) of covariate values affected the fit to logistic regression models used to estimate …

Sms Parent Action Intervention (Span): A Pilot Study To Assess The Feasibility Of Using Text Messaging To Promote Child Health On An American Indian Reservation, Julia M. Malich

Graduate Student Theses, Dissertations, & Professional Papers

Abstract

Purpose: Childhood obesity and its associated health risks are widely recognized as a major public health crisis in the United States (Johnson & Johnson, 2014; Proctor, 2008; Rogers et al, 2013; Vinci et al, 2016) and worldwide (Elías-Boneta, Toro, Garcia, Torres, & Palacios, 2015). Among children in low-income families, American Indian/Alaska Native (AIAN) children have the highest prevalence of obesity (21.1%) compared to other racial/ethnic groups (14.74% overall) (Pan et al, 2015). Parents play an important role in introducing healthy foods and encouraging physical activity in young children (Birch & Ventura, 2009; Lindsay et al., 2006; Natale et al., …

Poison In Pink, Sydney V. Cook

Graduate Student Theses, Dissertations, & Professional Papers

Humans slather, spray, mist, and cleanse their bodies with personal care products like lotion, hairspray, cologne, and shampoo every day. Our cupboards are stocked full of them, but few of us understand what is in those jars and bottles. We trust that if it’s on the shelf at the store, it’s safe. However, this is not always the case, and many personal care products contain chemicals that are harmful to human and environmental health.

My multi-disciplinary Environmental Studies thesis project combines evidenced-based research, interviews, nonfiction narrative, and science communication to create part of a book manuscript intended to educate general …