Life Sciences Commons^™

Very Rapid Onset Cannabis Dependence Risk In Relation To Co-Occurring Use Of Other Psychoactive Drugs, Olga A. Vsevolozhskaya, Fernando A. Wagner, James C. Anthony

Biostatistics Presentations

Background: Epidemiological estimates for lifetime cumulative incidence indicate that for every 9-11 who start using cannabis, one becomes a case of the cannabis dependence syndrome (CDS) – i.e., roughly 9%-11%. More recent estimates clarify that CDS risk might be much lower among ’cannabis only’ users, due in part to the fact that many ’cannabis only’ users try the drug a few times and never again. We turned to Hill functional analysis in order to study CDS probability soon after 1st cannabis use, estimated across strata defined by the number of recent days of cannabis use, with an acknowledgment that a …

Understanding The Importance Of Intermittently Fragmented Stream Habitat For Isolated Westslope Cutthroat Trout (Oncorhynchus Clarki Lewisi) In The Colville National Forest, Washington, Forrest Michael Carpenter

Dissertations and Theses

Climate change and anthropogenic effects have vastly reduced Westslope Cutthroat Trout (Oncorhynchus clarki lewisi, WCT) habitat throughout their range, including the Colville National Forest in northeastern Washington where this study was conducted. Many native salmonid populations have declined in abundance since the early 1900s due to a variety of climate- and human-driven forces. Westslope Cutthroat Trout are especially sensitive to habitat loss or degradation and to climate change. Together, climate change, habitat degradation, and non-native salmonid invasions are contributing to increasingly fragmented WCT populations. Ongoing and predicted future warming trends are expected to further fragment these populations and …

Efficient Method For Transfer Of Microinjected Eggs To Mouse Ampulla For Generating Transgenic Mice, Guang Wen, Jin Di, Qian Li, Jianling Chen, Ling Jin, Cheng Wang, Sanqing Xu

Publications and Research

Background: The new method described here is highly efficient in transferring microinjected mouse eggs (MEs) through the bursa membrane of a surrogate mother mouse to the ampulla of the oviduct without damaging the blood vessels on the bursa membrane.

Results: This method causes no loss of blood, and it produces newborn pups/founders from approximately 70% of the transferred MEs, because only a small hole is made on the blood vessel–free area of the bursa membrane and ampulla of the surrogate mother mouse. The infundibulum remains intact. The small hole on the bursa membrane/ ampulla may already heal up before the …

Interactions Among Climate, Topography And Herbivory Control Greenhouse Gas (Co2, Ch4 And N2o) Fluxes In A Subarctic Coastal Wetland, Kathy C. Kelsey, A. Joshua Leffler, Karen H. Beard, Joel A. Schmutz, Ryan T. Choi, J. M. Welker

Wildland Resources Faculty Publications

High-latitude ecosystems are experiencing the most rapid climate changes globally, and in many areas these changes are concurrent with shifts in patterns of herbivory. Individually, climate and herbivory are known to influence biosphere-atmosphere greenhouse gas (GHG) exchange; however, the interactive effects of climate and herbivory in driving GHG fluxes have been poorly quantified, especially in coastal systems that support large populations of migratory waterfowl. We investigated the magnitude and the climatic and physical controls of GHG exchange within the Yukon-Kuskokwim Delta in western Alaska across four distinct vegetation communities formed by herbivory and local microtopography. Net CO2 flux was greatest …

An Examination Of Student Outcomes In Studio Chemistry, Alan L. Kiste, Gregory E. Scott, Jesse Paul Bukenberger, Miles Markmann, Jennifer Moore

Chemistry and Biochemistry

Twenty years ago, a major curriculum revision at a large, comprehensive university in the Western United States led to the implementation of an integrated lecture/laboratory (studio) experience for our engineering students taking general chemistry. Based on these twenty years of experience, construction of four purpose-built studio classrooms to house the majority of the remaining general chemistry courses was completed in 2013. A detailed study of the effects of the entire ecology of the studio experience on student success was initiated at that time. Data from content knowledge pre- and post-tests, learning attitudes surveys, and student course evaluations show positive effects …

Trophic Ecology Of Green Turtles (Chelonia Mydas) From Dry Tortugas National Park, Florida, David C. Roche

HCNSO Student Theses and Dissertations

Located 100 km west of Key West, Florida, Dry Tortugas National Park (DRTO) is a largely untouched subtropical marine ecosystem that serves as an important developmental habitat, nesting ground, and foraging area for several species of sea turtles, including green turtles. The Park supports a recovering population of green turtles comprised of resident juveniles, subadults, and adults of both sexes; nesting females include residents and migrating females that only return to nest. Stable isotope analysis has been applied widely to describe the trophic ecology of green turtles, from urbanized bays with significant anthropogenic input, to relatively pristine ecosystems with healthy …

Weight Management Delivered Through An App, May Help Resolve Body Image Dissatisfaction In Women Enrolled In Wic, Alexis Ariel O’Connell

Honors Capstones

No abstract provided.

Copper Oxide Nanoparticles Impact Several Toxicological Endpoints And Cause Neurodegeneration In Caenorhabditis Elegans, Michael Joseph Mashock, Tyler Zanon, Anthony D. Kappell, Lisa N. Petrella, Erik C. Andersen, Krassimira R. Hristova

Biological Sciences Faculty Research and Publications

Engineered nanoparticles are becoming increasingly incorporated into technology and consumer products. In 2014, over 300 tons of copper oxide nanoparticles were manufactured in the United States. The increased production of nanoparticles raises concerns regarding the potential introduction into the environment or human exposure. Copper oxide nanoparticles commonly release copper ions into solutions, which contribute to their toxicity. We quantified the inhibitory effects of both copper oxide nanoparticles and copper sulfate on C. elegans toxicological endpoints to elucidate their biological effects. Several toxicological endpoints were analyzed in C. elegans, including nematode reproduction, feeding behavior, and average body length. We examined three …

Bird Response To Land Use Change In Northern Argentina, David J. Flaspohler

TechTalks

Land use change is responsible for changes in wildlife populations including declines for many species. When one land cover type is converted into another for forestry, agriculture or pasture, some bird species are harmed while others may be favored. Understanding how biodiversity responds to land use change is essential to allowing us to anticipate and respond to management options and economic drivers of change. We studied the response of a diverse bird community in an historically grassland portion of northern Argentina, where eucalyptus plantations are expanding rapidly to support timber, pulp and incipient bioenergy industries. Plantations contained the fewest bird …

Preliminary Examination Of The Bacterial Diversity Harbored By The Pill Bug (Armadillidium Vulgare), Sondos Alhajouj

Theses & Dissertations

Pill bugs (Armadillidium vulgare) were examined at 4 time points during 2015- 2016. The time points corresponded to summer, fall, winter, and spring. At each time point, 2 to 10 pill bugs were collected from each of three sites. All sites were located in San Antonio, Texas. The first site was in central San Antonio, but due to difficulties in collecting specimens the first site was only utilized at the first time point. The remaining localities, Bonilla Science Hall on the campus of the University of the Incarnate Word and a private residence in northwest San Antonio, were …

The Effect Of Varying Environmental Conditions On The Growth Of Stenotrophomonas Maltophilia, Norah Alqahtani

Theses & Dissertations

Stenotrophomonas maltophilia has emerged as an important opportunistic pathogen in the debilitated host. It possesses the ability to target and colonize respiratory-tract epithelial cells and grow on the surface of medical devices. It is associated with a significant case-fatality ratio in cystic fibrosis patients. S. maltophilia species have the ability to grow in bacterial biofilms, which further complicates these infections by providing the opportunity for acquisition of antibiotic resistant genes. This study investigated the effects on the growth of different strains of the same species of S. maltophilia, ATCC 51331 and 13637, when the selected environmental factors are varied. …

Seeing The Invisible: Revealing Atrial Ablation Lesions Using Hyperspectral Imaging Approach, Narine Muselimyan, Luther Swift, Huda Asfour, Tigran Chahbazian, Ramesh Mazhari, Marco Mercader, Narine Sarvazyan

Pharmacology and Physiology Faculty Publications

Background

Currently, there are limited means for high-resolution monitoring of tissue injury during radiofrequency ablation procedures.

Objective

To develop the next generation of visualization catheters that can reveal irreversible atrial muscle damage caused by ablation and identify viability gaps between the lesions.

Methods

Radiofrequency lesions were placed on the endocardial surfaces of excised human and bovine atria and left ventricles of blood perfused rat hearts. Tissue was illuminated with 365nm light and a series of images were acquired from individual spectral bands within 420-720nm range. By extracting spectral profiles of individual pixels and spectral unmixing, the relative contribution of ablated …

Novel Aspects Of Drosophila Suzukii (Diptera: Drosophilidae) Biology And An Improved Method For Culturing This Invasive Species With A Modified D. Melanogaster Diet, Blair J. Sampson, Trevor Mallette, Karla M. Addesso, Oscar E. Liburd, Lindsy E. Iglesias, Stephen J. Stringer, Chris T. Werle, Donna A. Shaw, Drew Larsen, John J. Adamczyk

Agricultural and Environmental Sciences Faculty Research

Drosophila suzukii (Matsumara) (Diptera: Drosophilidae), the spotted wing drosophila, is a global pest of soft fruits now rearable on a standard D. melanogaster (Meigen) diet containing the fly's own natural food: soft-skinned berries. The techniques tested here can save 40% of cultures from microbial contamination that develops after combining artificial food sources (e.g., standard drosophila media) with unsterilized host plant material (berries). A suitable ratio for mixing dietary ingredients for a vial or test-tube rearing system includes, by weight, 1 part berry tissue for oviposition, 1.5 parts dry diet media for carbohydrate, 7 parts clean water for moisture, and ∼5 …

Cows - Clean Ocean Wave Sculpture, Kim Bernard

Artist in Residence: Kim Bernard

Description and images from Kim Bernard's University of New England Artist in Residence fall semester 2016 culminating project. Kim worked with UNE students and faculty to gather ocean debris and weave it into a permanent art installment at UNE's Arthur P. Girard Marine Science Center.

Billbug (Sphenophorus Spp.) Chemical Ecology And Seasonal Biology In Indiana Turfgrass, Alexandra G. Duffy

Open Access Theses

Billbugs (Coleoptera: Curculionoidea: Sphenophorus) are serious pests of managed turfgrass across North America. Damage symptoms are most visible during stressful periods of the growing season and are commonly confused with disease, drought, or nutrient deficiency. Billbugs are frequently a perennial problem and when misdiagnosed, damage often results in seriously degraded stands of turfgrass that are easily encroached by weeds. Presently, management of billbugs relies heavily on chemical insecticides. Even then, the nationwide assemblage of multiple sympatric billbug species and the cryptic nature of the damaging larval stage makes management of these insects challenging. A better understanding of billbug biology …

Resource Assessment Report Western Rock Lobster Resource Of Western Australia, Simon De Lestang, Nick Caputi, Jason R. How

WA Marine Stewardship Council report series

The western rock lobster Panulirus cygnus (George) is taken by commercial and recreational fishers throughout its geographic range along the lower west coast of Western Australia. The main commercial fishery for P. cygnus is the West Coast Rock Lobster Managed Fishery (WCRLMF), which is Australia’s largest single-species fishery, currently worth about $400 million annually. Western rock lobster provides the basis for the economies of a number of coastal towns and also supports a recreational fishery.

Multitaxon Distribution Models Reveal Severe Alteration In The Regional Biodiversity Of Freshwater Invertebrates, Charles P. Hawkins, Lester L. Yuan

Watershed Sciences Faculty Publications

Knowledge of current and historical conditions is needed to guide conservation and restoration policies, but such information is lacking for most taxa. This problem is especially severe for small, inconspicuous taxa, such as the thousands of invertebrate species that inhabit stream and other freshwater ecosystems. We describe a novel application of River Invertebrate Prediction and Classification System (RIVPACS)-type multitaxon distribution models that, when applied to the biological survey data routinely collected in support of water-quality monitoring programs, can quantify the regional biodiversity status of hundreds of taxa. We used models developed for 2 stream data sets (North Carolina and the …

Game Theory Analysis Of Intra-District Water Transfers; Case Study Of The Berrenda Mesa Water District, Harry Riordan Ferdon

Master's Theses

California state officials have continued to warn and encourage preparedness for the growing threats of water scarcity. This puts pressure on water suppliers to develop technological and managerial solutions to alleviate the problems associated with scarcity. A recent popular management strategy for distributing water is encouraging water transfers. While there has been analyses on water transfers between large districts and agencies, little analysis has been completed for smaller scale trades, i.e. between individuals in the same water district. This analysis models an agricultural water district, based on the Berrenda Mesa Water District (BMWD). In the model, the growers in the …

Functional Trait Values, Not Trait Plasticity, Drive The Invasiveness Of Rosa Sp. In Response To Light Availability, Rebecca E. Drenovsky, Jennifer E. Murphy

Biology

PREMISE OF THE STUDY : Functional trait plasticity in resource capture traits has been suggested as an underlying mechanism promoting invasive species establishment and spread. Earlier studies on this mechanism treat invasiveness as a discrete characteristic (i.e., invasive vs. noninvasive) and do not consider the potential impacts of evolutionary history. In the present study, we used a continuous measure of invasiveness and a phylogenetic framework to quantify the relationship between functional trait expression, plasticity, and invasiveness in Rosa .

Stopover On Galapagos During Autumn Migration Of Bobolinks (Dolichonyx Oryzivorus), Noah G. Perlut, Rosalind Renfrew

Environmental Studies Faculty Publications

The Bobolink (Dolichonyx oryzivorus) is the only landbird species that is known to stop every year in Galapagos while migrating; however, its stopover ecology while on the islands is unknown. In October 2015, we searched for and captured Bobolinks in the highlands of San Cristóbal. We found Bobolinks in two fields, separated by 9.15 km, at ∼425 m elevation. Average daily counts of Bobolinks on these two fields were 3.2 ± 1.8 and 4.8 ± 2.3 individuals. We caught nine individuals; body mass and fat reserves varied from 22.5–40.0 g and no fat reserves to 50–100% reserves, respectively. …

A Comparison Of Multipath And Conventional Neuromuscular Electrical Stimulation, Cody Brian Bremner

Dissertations

Neuromuscular electrical stimulation (NMES) is the use of an electrical current for the purpose of eliciting a muscular response, and these treatments are most often used clinically for the specific purpose of increasing quadriceps strength. It is commonly accepted that the effectiveness of NMES for this purpose is primarily determined by the NMES training intensity. However, spatially limited motor unit recruitment, fatigue and discomfort negatively impact NMES-induced torque, which subsequently reduces NMES training intensities. Due to the importance of NMES training intensity, a substantial amount of research has focused on strategies designed to increase NMES-induced torque production, as well as …

Examining The Combined Effects Of Dissolved Oxygen, Temperature, And Body Size On The Physiological Responses Of A Model Macrobenthic Polychaete Species, Capitella Teleta, Kelsey Burns Gillam

Dissertations

While the scientific community is in consensus that coastal systems are threatened by climate change, few climate change studies test the effects of more than one variable directly related to climate change. The dissolved oxygen (DO) levels of the ocean are currently subject to both global warming and eutrophication; 94% of all hypoxia zones are expected to experience >2°C increase by 2035. This dissertation aims to examine how a model organism responds to simultaneous thermal and DO stress involving four levels of DO (100%, 70%, 50%, and 20%) saturation and three temperatures (15°C, 20°C, and 25°C).

The polychaete, Capitella teleta …

Epidemiology Of White Spot Syndrome Virus In The Daggerblade Grass Shrimp (Palaemonetes Pugio) And The Gulf Sand Fiddler Crab (Uca Panacea), Muhammad

Dissertations

Ever since the first outbreaks of White spot syndrome virus (WSSV), which causes White Spot Disease (WSD), in Asia in the early 1990s, the pathogen has been a major constraint to the profitability of the shrimp aquaculture industry across the globe. WSSV has a broad host range and is routinely detected in wild decapod crustaceans. In the present study, two common species in the tidal salt marsh along the northern coast of the Gulf of Mexico, the daggerblade grass shrimp (Palaemonetes pugio) and the Gulf sand fiddler crab (Uca panacea), were investigated for their role as …

A Microbiomic Approach To The Characterization Of The Impacts And Influences Of Viral, Bacterial, And Harmful Algal Bloom Toxins On The Bottlenose Dolphin, Corey David Russo

Dissertations

As apex predators that display high site fidelity Tursiops truncatus (bottlenose dolphin) are indicators of marine ecosystem health. Bottlenose dolphins, additionally, display pathogenesis and immune response similar to that of humans. Humans and coastal bottlenose dolphins, in particular, are constantly exposed to the same industrial, agricultural and domestic toxins and pathogens, contaminants and pollutants. Thus, studies on the bottlenose dolphin are also valuable in bridging the gap between ocean and human health. Bottlenose dolphins are susceptible to viral bacterial and toxin infection. Infection in the bottlenose dolphin manifests itself in the form of mass stranding events, unusual mortality events, chronic …

Punctuated Evolution Within A Eurythermic Genus (Mesenchytraeus) Of Segmented Worms: Genetic Modification Of The Glacier Ice Worm F1f0 Atp Synthase, Shirley A. Lang

Graduate School of Biomedical Sciences Theses and Dissertations

Segmented worms (Annelida) are among the most successful animal inhabitants of extreme environments worldwide. An unusual group of Mesenchytraeus worms endemic to the Pacific Northwest of North America occupy geographically proximal ecozones ranging from low elevation temperate rainforests to high altitude glaciers. Along this altitudinal transect, Mesenchytraeus representatives from disparate habitat types were collected and subjected to deep mitochondrial and nuclear phylogenetic analyses. Evidence presented here employing modern bioinformatic analyses (i.e., maximum likelihood, Bayesian inference, multi-species coalescent) supports a Mesenchytraeus “explosion” in the upper Miocene (5-10 million years ago) that gave rise to ice, snow and terrestrial worms, derived from …

Graduate Studies Newsletter, Graduate Studies Office

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Progression Magazine, 2016 Winter, Coastal Carolina University

Progression Magazine

Magazine of the College of Science at Coastal Carolina University.

Ua12/9 Newsletter, Vol. 3, Issue 8, Wku Counseling & Testing Center

WKU Administration Documents

Newsletter created by and about WKU Counseling & Testing Center.

• Laves, Karl. Therapy Is Going to the Dogs (and Cats, Horses, the Occasional Bunny)

Synthesis And Performance Of Novel Supramolecular Tools For Single-Particle Cryogenic Electron Microscopy And Drug And Gene Delivery, Kyle J. Wright

Open Access Dissertations

High-resolution biomacromolecular structure elucidation is fundamentally important to structure-based drug design and basic research into complex biochemical processes. Cryo-EM is an emerging alternative to XRD and NMR that is complementary in many ways relative to XRD and NMR. Materials approaches to cryo-EM are anticipated to greatly facilitate the cryo-EM process, allowing progress toward a more high-throughput application of cryo-EM to address challenges in structural biology. ^ Various affinity-based approaches inspired by approaches previously introduced for 2D crystallization were developed for facilitation of cryo-EM. A library of affinity lipopolymer constructs were synthesized consisting of lipopolymers of various PEG molecular weights conjugated …

Complex Glycan Utilization Preferences Of Human Gut Bacteria, Yunus Emre Tuncil

Open Access Dissertations

Complex glycans, making up dietary fiber, have gained significance in recent years as they are the main energy source for the colonic microbiota which are physiologically important for the host health. Understanding glycan utilization strategies of the members of this community is essential to maximize beneficial health outcomes obtained from them. In order to investigate how the members of our gut microbiota utilize glycans and activate their respective polysaccharide utilization loci (PULs), we performed a series of time-course assays in which two model organisms, Bacteroides thetaiotamicron (Bt) and B. ovatus (Bo), were individually grown in a medium containing carbohydrates utilized …