Life Sciences Commons^™

Low Energy Availability In Exercising Men Is Associated With Reduced Leptin And Insulin But Not With Changes In Other Metabolic Hormones, Karsten Koehler, Neele R. Hoerner, Jenna C. Gibbs, Christoph Zinner, Hans Braun, Mary Jane De Souza, Wilhelm Schaenzer

Department of Nutrition and Health Sciences: Faculty Publications

Low energy availability, defined as low caloric intake relative to exercise energy expenditure, has been linked to endocrine alterations frequently observed in chronically energy-deficient exercising women. Our goal was to determine the endocrine effects of low energy availability in exercising men. Six exercising men (V_O2peak : 49.3 ± 2.4 ml · kg⁻¹ · min⁻¹) underwent two conditions of low energy availability (15 kcal · kg⁻¹ fat-free mass [FFM] · day⁻¹) and two energy-balanced conditions (40 kcal · kg⁻¹ FFM · day⁻¹) in randomized order. During one low energy availability and …

Carbohydrate Intake In Form Of Gel Is Associated With Increased Gastrointestinal Distress But Not With Performance Differences Compared With Liquid Carbohydrate Ingestion During Simulated Long-Distance Triathlon, Mahdi Sareban, David Zügel, Karsten Koehler, Paul Hartveg, Martina Zügel, Uwe Schumann, Jürgen Michael Steinacker, Gunnar Treff

Department of Nutrition and Health Sciences: Faculty Publications

The ingestion of exogenous carbohydrates (CHO) during prolonged endurance exercise, such as long-distance triathlon, is considered beneficial with regard to performance. However, little is known about whether this performance benefit differs among different forms of CHO administration. To this end, the purpose of our study was to determine the impact of CHO ingestion from a semisolid source (GEL) on measures of performance and gastrointestinal (GI) comfort compared with CHO ingestion from a liquid source (LIQ). Nine well-trained triathletes participated in this randomized crossover study. Each participant completed a 60-min swim, 180-min bike exercise, and a 60-min all-out run in a …

Suppression Of Nlrp3 Inflammasome By Γ-Tocotrienol Ameliorates Type 2 Diabetes, Yongeun Kim, Wei Wang, Meshail Okla, Inhae Kang, Regis Moreau, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

The Nod-like receptor 3 (NLRP3) inflammasome is an intracellular sensor that sets off the innate immune system in response to microbial-derived and endogenous metabolic danger signals. We previously reported that γ-tocotrienol (γT3) attenuated adipose tissue inflammation and insulin resistance in diet-induced obesity, but the underlying mechanism remained elusive. Here, we investigated the effects of γT3 on NLRP3 inflammasome activation and attendant consequences on type 2 diabetes. γT3 repressed inflammasome activation, caspase-1 cleavage, and interleukin (IL) 1β secretion in murine macrophages, implicating the inhibition of NLRP3 inflammasome in the anti-inflammatory and antipyroptotic properties of γT3. Furthermore, supplementation of leptin-receptor KO mice …

Soft Drinks Consumption And Child Behaviour Problems: The Role Of Food Insecurity And Sleep Patterns, Christian King

Department of Nutrition and Health Sciences: Faculty Publications

Objective: To examine whether the association between soft drinks consumption and child behaviour problems differs by food security status and sleep patterns in young children.

Design: Cross-sectional observational data from the Fragile Families and Child Wellbeing Study (FFCWS), which collected information on food insecurity, soft drinks consumption, sleep patterns and child behaviour problems. Bivariate and multivariate ordinary least-squares regression analyses predicting child behaviour problems and accounting for socio-economic factors and household characteristics were performed.

Setting: Twenty urban cities in the USA with a population of 200 000 or more.

Subjects: Parental interviews of 2829 children who were about 5 years …

The Role Of Microrna In Cardioprotection: Ischemic Preconditioning And Mesenchymal Stem Cell Paracrine Effects, Kristin Luther

Dissertations

Changes in gene expression and protein levels are an important aspect of cardioprotection in which short non-coding RNA known as miRNA may play a key regulatory role. We investigated the functions of several miRNAs in the context of two cardioprotective stimuli, ischemic preconditioning (IPC) and mesenchymal stem cell (MSC) paracrine effects. We hypothesized that downregulation of a set of miRNAs (miR-148a/b, miR-30b, and let-7a*) augments expression of protective heat shock proteins during IPC, and that MSC exosomes transfer miR-21 to cardiomyocytes, resulting in downregulation of pro-apoptotic genes and reduction of infarct size.

IPC increased the level of Hsp70, Hsp90, and …

The Amino Terminal Region Of Cardiac Myosin Binding Protein-C Is Necessary For Cardiac Function, Thomas Lawrence Lynch

Dissertations

Cardiac myosin binding protein-C (cMyBP-C) is a thick filament-associated protein that has been suggested to regulate cardiac contraction via its amino terminal (N’) region. Following ischemic injury to the heart, cMyBP-C is cleaved into a predominant N’ fragment consisting of domains C0 through C1 and the first 17 residues of the M-domain that is referred to as C0-C1f. However, the necessity of the N’-C0-C1f region of cMyBP-C in regulating cardiac function in vivo has not been elucidated. I hypothesized that the N’-C0-C1f region of cMyBP-C is critical for normal cardiac function in vivo. To test this hypothesis, transgenic (TG) mice …

Regulation Of The Subunits Of Hypoxia Inducible Factors By Sprouty2 And Its Impact On Different Biological Processes, Kristin Caroline Hicks

Dissertations

The hypoxia inducible factors (HIF1α, HIF2α, HIF1β) promote transcription of genes that regulate glycolysis and cell survival and growth. Sprouty2 (Spry2) is a modulator of receptor tyrosine kinase signaling and inhibits cell proliferation via different mechanisms. Because of the seemingly opposite actions of the HIF and Spry2 on cellular processes, we hypothesized that Spry2 decreases the protein levels of HIF1α, HIF2α and HIF1β by enhancing the proximity of the HIF subunit to an ubiquitin ligase capable of degrading the subunit. Focusing on HIF1α as a prototypical alpha subunit, in a variety of tumor derived cell lines, Spry2 decreases the protein …

Heart And Sole: The Functional Role Of Fast-Skeletal Myosin Binding Protein-C In Cardiac And Skeletal Muscle, Brian Leei Lin

Dissertations

The goal of my dissertation was to compare and contrast the function of all three major isoforms of Myosin Binding Protein-C (MyBP-C): slow-skeletal, fast-skeletal, and cardiac (ssMyBP-C, fsMyBP-C, and cMyBP-C, respectively), with a focus on the least characterized isoform, fsMyBP-C. Using a variety of ex vivo, in vitro, and in silico methods, my research demonstrated that the N-terminal region of all MyBP-C isoforms bind to actin and shift tropomyosin, thus activating the thin filament during contraction. Furthermore, each isoform differentially activated the thin filament over isoform-specific ranges of Ca2+: slow-skeletal activates at low Ca2+, fast-skeletal activates at higher Ca2+, and …

The Impact Of Altered T Cell Receptor—Peptide-Major Histocompatibility Complex Interactions On Antigen Recognition And T Cell Function, Timothy T. Spear

Dissertations

Adoptive cell transfer (ACT) using T cell receptor (TCR) gene-modified T cells is an exciting and rapidly evolving field. Numerous basic science and clinical studies have demonstrated various levels of feasibility, safety, and efficacy using TCR-engineered T cells to treat cancer and viral infections. Genomic instability of targeted diseases, however, requires effective and safe TCRs to cross-recognize mutated antigens while minimizing on- or off-target toxicities. Thus, improvements to T cell-based therapeutics mandate a broader understanding of the principles governing antigen recognition. This dissertation addresses critical biologic questions evaluating which parameters are most important in facilitating antigen recognition, and how alterations …

Inhibition Of B Lymphopoiesis By Adipocytes And Myeloid-Derived Suppressor Cells, Domenick Kennedy

Dissertations

B lymphopoiesis declines with age in humans, mice, and rabbits. Impaired B lymphopoiesis correlates with increased fat in the bone marrow (BM), suggesting that adipocytes negatively regulate this process. In fact, adipocyte factors were found to inhibit B cell development in BM cultures.

Our goal was to understand the mechanism by which adipocytes inhibit B cell development. Through culturing mouse BM cells on OP9 stromal cells in the presence of adipocyte-conditioned medium (ACM), we found that adipocytes promote the accumulation of CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs). These cells were not simply bystanders, as we report for the first time that …

Adventist Affiliation And Type 2 Diabetes Pre- And Post-Complete Health Improvement Program (Chip), Janie Unruh

Walden Dissertations and Doctoral Studies

Adventists following a plant-based diet have half the prevalence and incidence of type 2 diabetes than nonvegetarian Adventists. This study used a quantitative, correlational study design to assess if there was a significant difference in type 2 diabetes prevalence rate between Adventists and non-Adventists preprogram, and if there were significant differences in biometrics between Adventists and non-Adventists with diabetes pre- and post-Complete Health Improvement Program (CHIP). This study incorporated the social ecological model for its conceptual framework and examined pre- and postprogram changes among Adventists (n=210; 20.1%) and non-Adventists (n=836; 79.9%) with type 2 diabetes. It used secondary data from …

Promoting Diabetes Self-Managment Eduaction And Training In Out-Patient Clinics, Inemesit Godwin Udo

Walden Dissertations and Doctoral Studies

Abstract

Type 2 diabetes mellitus (T2DM) is a long term metabolic disorder characterized by high blood sugar, insulin resistance, and relative lack of insulin. T2DM is a leading cause of cardiovascular disease, blindness, kidney failure, lower-limb amputation, and other complications that are costly to patients and the U.S. health care system. Lack of knowledge and underdeveloped skills for self-management of diabetes continues to be the biggest problem for patients with T2DM. Using a team approach and Rosswurm and Larrabee's (1999) conceptual model as a framework, the purpose of this doctorate of nursing practice quality improvement project was to develop an …

Exploring Factors Influencing Information Technology Portfolio Selection Process In Government-Funded Bioinformatics Projects, Braulio J. Cabral

Walden Dissertations and Doctoral Studies

In 2012, the National Cancer Institute's (NCI) Board of Scientific Advisors (BSA) conducted a review of the Center for Biomedical Informatics and Information Technology's (CBIIT) bioinformatics program. The BSA suggested that the lack of a formal project selection process made it difficult to determine the alignment of projects with the mission of the organization. The problem addressed by this study was that CBIIT did not have an in-depth understanding of the project selection process and the factors influencing the process. The purpose of this study was to understand the project selection process at CBIIT. The research methodology was an exploratory …

Influences Of Nutritional Food Label Understanding In African-American Women With Obesity, Cynthia Jean Hickman

Walden Dissertations and Doctoral Studies

Nutritional food label understanding (NFLU) in African American Women (AAW) is a philosophy that addresses obesity. Public health efforts have implemented nutritional and caloric information to packaged and restaurant foods to improve nutrient and calorie literacy. Research suggest NFLU might have a minimal effect on reducing obesity. However, it is not known how obese AAW born during the baby-boom era (51 to 64 years of age) perceive NFLU in relation to their dietary behaviors. The purpose of this phenomenological study was to understand the lived experience of obese AAW regarding NFLU from a cognitive and behavioral perspective. Twelve, AAW answered …

Equine Facilitated Psychotherapy For Veteran Survivors With Full Or Partial Ptsd, Mark Aaron Mayfield

Walden Dissertations and Doctoral Studies

Symptom severity among veteran survivors with partial or full posttraumatic stress disorder (PTSD) continues to increase, with approximately 40% of U.S. veterans reporting significant symptomology 10 years after initial onset of the condition. Veteran survivors often struggle to find therapeutic interventions that meet their specific needs and have a difficult time maintaining a therapy that is both equitable and evidenced based. Grounded in the Rogerian, client-centered theory, the purpose of this qualitative collective exploratory case study was to explore the effects of equine-facilitated psychotherapy with 3 veteran survivors with partial or full PTSD. A 4-stage process was used to collect …

Performance Measures Of Road Crossing Structures From Relative Movement Rates Of Large Mammals, Adam Andis

Graduate Student Theses, Dissertations, & Professional Papers

In recent decades, there has been an increase of engineering projects that seek to mitigate the barrier effect roads impose on wildlife by installing wildlife crossing structures that promote permeability of the road corridor. The 41 fish and wildlife crossing structures installed along a 90km stretch of US Highway 93 on the Flathead Indian Reservation in western Montana, represent one of the most extensive of such projects in North America. As mitigation efforts are increasingly considered and implemented in road construction projects, the need to assess these structures’ effectiveness grows. This study is the first to compare observations of animal …

Baseline Study Of Recovering Headwater Streams In The Sapphire Mountains, Western Montana, Morgan Vinyard

Graduate Student Theses, Dissertations, & Professional Papers

Many riparian areas in the western United States have been degraded by grazing and logging. In the year 2000, the Montana Department of Environmental Quality (DEQ) re-initiated an effort to identify and characterize reference streams, or least impacted streams in each region of the state. In Montana’s Bitterroot Valley, most streams have been impacted by grazing, irrigation, and timber production. The Montana DEQ has not been able to identify reference streams in the Sapphire Mountains on the east side of this valley. MPG Ranch, located on the west-facing slopes of the Sapphire Mountains and purchased in 2009 by a conservation-minded …

Spatio-Temporal Analysis Of Ungulate Browsing On Willow (Salix Spp.) Communities Within The Northern Range Of Yellowstone, Donald L. Belile Jr.

Graduate Student Theses, Dissertations, & Professional Papers

Variation in ungulate browsing intensity on willow (Salix spp.) communities across Yellowstone’s northern range has been attributed to various biophysical factors including climate, topography, and predators. These studies were conducted without a long-term browsing dataset; they lacked a spatially extensive sampling design, and did not include dynamic snow characteristics known to affect ungulate mobility, habitat selection, and foraging strategies. We therefore focused on snow, an extreme landscape level disturbance that places nutritional stress on ungulates during the winter when most browsing on shrubs occurs. We applied a non-destructive sampling method by consecutively recording whether stem leaders were browsed between …

Conservation Genetics On The Frontline, Kenneth W. Rand

Graduate Student Theses, Dissertations, & Professional Papers

Conservation genetics stands out as an effective tool for discovering and monitoring rare, endangered or invasive populations of plants and animals. Particularly when compared to traditional search and capture methods, it provides more holistic studies to preserve the disappearing biodiversity of the American West and the world.

Three stories highlight the work done to preserve biodiversity through the use of conservation genetics:

1. Trout Rescue: A new hope for westslope cutthroat in Montana How to save a disappearing westslope cutthroat trout through genetic rescue by adding genetic diversity to ensuring future survival in increasingly warming waters more harm than good. …

Impacts Of Recreational Aviation On Wildlife: The Physiological Stress Response In White-Tailed Deer (Odocoileus Virginianus) And Associated User Perceptions, Devin W. Landry

Graduate Student Theses, Dissertations, & Professional Papers

Recreational aviation on public lands may negatively impact wildlife. However, land-use decisions need to balance user need with wildlife impact. We know very little about 1) how back country airstrip use affects local wildlife, or 2) attitudes and perceptions of recreational pilots toward possible management actions. For my Master’s research, I investigated how aircraft activity influenced physiological measures of stress in white-tailed deer, while also modeling how psychometrics such as wildlife attitudes and place attachment predict the willingness of recreational pilots engage in impact-mitigating behaviors. I measured physiological stress through non-invasive sampling of stress hormones in fecal samples (fecal glucocorticoid …

Long-Term Impacts Of Fuel Treatments On Tree Growth And Aboveground Biomass Accumulation In Ponderosa Pine Forests Of The Northern Rocky Mountains, Kate A. Clyatt

Graduate Student Theses, Dissertations, & Professional Papers

In western North America, many low-elevation, dry forest types historically experienced frequent, low-severity fires. However, European settlement and fire suppression policies have contributed to over a century of fire exclusion, substantially altering forest structure and composition. There is considerable interest in restoring fire resilient characteristics to these forests through fuel reduction treatments. One limitation of current research on the impacts of fuel treatments is treatment longevity, as few studies have been able to quantify long-term responses to commonly applied treatments. This research evaluated tree growth and aboveground biomass responses 23 years after treatment in two silvicultural installations with different underburning …

Density Management In Young Western Larch Stands: Tree Growth, Stand Yield, And Carbon Storage 54 Years After Thinning, Michael S. Schaedel

Graduate Student Theses, Dissertations, & Professional Papers

Long-term silvicultural experiments can be used to test novel ecological hypotheses and answer contemporary management questions that were not envisioned at study initiation. We used a 54-year old western larch precommercial thinning (PCT) study in northwest Montana to examine two sets of questions: (1) how different PCT regimes affect long-term stand yield and tree growth, and (2) how PCT affects total aboveground carbon (C) storage and distribution among C pools. The study has three target densities (494 trees ha^-1, 890 trees ha^-1, and 1680 trees ha^-1) and three numbers of entries to achieve those …

Resin Duct Defenses In Ponderosa Pine During A Mountain Pine Beetle Outbreak: Genetic Effects, Mortality, And Relationships With Growth, Sean Pinnell

Graduate Student Theses, Dissertations, & Professional Papers

Despite formidable tree defenses against insect pests, including resin produced in resin ducts, bark beetles have recently caused extensive outbreaks in western North American forests. To date, our understanding of the extent to which resin duct defenses remain effective at the peak of an outbreak, the relative genetic vs. environmental controls on resin duct production, the degree to which drought reduces resin defenses in naturally occurring outbreaks, and whether and when defense production competes for resources with growth remains limited. Resolving these questions is relevant in the face of climate change, and increased risk of outbreaks. I took advantage of …

Positive Interactions In Temperate And Tropical Alpine Plant Communities: The Role Of Facilitation In Species-Specific Interactions, Invasion, And Landscape-Scale Distribution, Nicole Hupp

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Assessment Of Celecoxib Poly(Lactic-Co-Glycolic) Acid Nanoformulation On Drug Pharmacodynamics And Pharmacokinetics In Rats, Sam Harirforoosh, Kelsi O. West, Derek E. Murrell, James W. Denham, Peter C. Panus, Greg A. Hanley

Pharmacy Faculty Articles and Research

OBJECTIVE: Celecoxib (CEL) is a nonsteroidal anti-inflammatory drug (NSAID) showing selective cycloxygenase-2 inhibition. While effective as a pain reducer, CEL exerts some negative influence on renal and gastrointestinal parameters. This study examined CEL pharmacodynamics and pharmacokinetics following drug reformulation as a poly(lactic-co-glycolic) acid nanoparticle (NP).

MATERIALS AND METHODS: Rats were administered either vehicle (VEH) (methylcellulose solution), blank NP, 40 mg/kg CEL in methylcellulose, or an equivalent NP dose (CEL-NP). Plasma and urine (over 12 hrs) samples were collected prior to and post-treatment. The mean percent change from baseline of urine flow rate along with electrolyte concentrations in plasma …

Performance And Morphology In Sequoiadendron Genotypes Outside Of Their Range, Christopher M. Valness

Cal Poly Humboldt theses and projects

In its current fragmented distribution in 75 groves along the western slope of the Sierra Nevada, Sequoiadendron giganteum (SEGI) may be vulnerable to extreme shifts in environmental conditions such as warming temperatures and drought stress, which may reduce the already limited habitat for SEGI in native groves. Interest in outplanting of this iconic species for the objectives of genetic conservation and timber utilization due to decay resistance of heartwood would be supported by information on population variation to inform seed collection for these plantings. To that end, I assessed three SEGI common-garden trials which had been planted in spring 1981 …

The Effectiveness Of Physical Activity Interventions On Young Individuals With Autism Spectrum Disorder: A Meta-Analysis, Adam Nacario

Cal Poly Humboldt theses and projects

Objective: The purpose of the study is to examine the efficacy of physical activity interventions and practices on psychomotor, cognitive, or affective outcomes on young individuals diagnosed with Autism Spectrum Disorder (ASD).

Methods: Standard meta-analytical procedures determining inclusion criteria, literature searches in electronic databases, coding procedures, and statistical methods were used to identify and synthesize articles retained for analysis. Cohen’s d (1988) will be utilized in order to interpret effect sizes and quantify research findings. The impact was assessed using coding procedures that categorize independent variables.

Results: A total of 23 independent samples (N=896) were utilized in this analysis. Results …

Seasonal Habitat Selection Of The North American Porcupine (Erethizon Dorsatum) In A Coastal Dune Forest, Cara L. Appel

Cal Poly Humboldt theses and projects

Wildlife-habitat relationship studies are important for understanding the factors that determine where species occur in space and time. Habitat selection by generalist species should be studied on fine spatial and temporal scales to avoid masking important differences between seasons, localities, or orders of selection. I conducted the first study of habitat use and general ecology of North American porcupines (Erethizon dorsatum) in a coastal dune environment. Specifically, I assessed changes in body mass, home range size, and habitat selection in relation to the potential for seasonal nutritional and survival bottlenecks as reported elsewhere. Although they are considered generalists, …

Early Seral Mixed-Conifer Forest Structure And Composition Following A Wildfire Reburn In The Sierra Nevada, Erin Alvey

Cal Poly Humboldt theses and projects

Before the era of modern fire suppression, California’s northern Sierra Nevada mixed-conifer and yellow pine forests were self-regulating; recurring short-interval, low-mixed severity wildfires maintained forest structure and composition, which in turn exerted bottom-up controls on subsequent wildfires. As a result of fire suppression, and coupled with the effects of climate warming and other anthropogenic disturbances, the fundamental structure of mixed-conifer and yellow pine forests has shifted. Wildfires may now be increasing in size, severity, and frequency across western North America. However, little is known about the post-fire impacts of repeat wildfire on a forest after a long era of suppression. …

Evaluating Brandt’S Cormorant (Phalacrocorax Penicillatus) Reproductive Success: Effects Of Parental Care Behaviors And Estimating Individual Chick Survival, Shannon Murphy

Cal Poly Humboldt theses and projects

Variation in reproductive success is widely measured in seabird biology in an effort to indicate changes in the marine environment, or understand basic questions about ecology or conservation of seabirds. When variation in seabird reproductive success is suggested to indicate changes in the marine environment without identifying the proximate causes of such variation, inference is limited to association, and the proximate causes themselves could prove more effective as indicators. My study informs this problem by examining and quantifying proximate causes of variation in reproductive success, at the level of nests and individual chicks.

I used video of Brandt’s Cormorant ( …